LC-2000Plus Series. High Performance Liquid Chromatograph
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1 LC-2000Plus Series High Performance Liquid Chromatograph
2 LC-2000Plu The JASCO Corporation was founded in 1958 to produce optical spectroscopy products for University research departments. The experience gained by JASCO in both optical design and computer technology led to the production of spectrophotometric detectors for HPLC. The move into the HPLC market continued with the production of solvent delivery systems, gradient elution devices and a complete range of detectors. JASCO has more than 30 years experience in the design and development of innovative chromatography instrumentation for a wide range of applications. A worldwide network of companies supports the full range of analytical instrumentation in educational, industrial, quality control and research laboratories. The latest development is the LC-2000 series High Performance Liquid Chromatograph which meets the key requirements of today's analytical laboratory. 2
3 s HPLC system building on success 3
4 LC-2000Plus system Micro, analytical and preparative solvent delivery systems Versatile autosamplers Solvent switching, mixing and degassing Column ovens Wide range of detectors Isocratic systems Low pressure gradient systems 4
5 flexibility High pressure gradient systems Compact design to maximise laboratory bench space Safe-stacking modular architecture PC system or direct keypad control 5
6 Your HPLC system re Speed Minimum set-up time, ease of use Productivity Assured throughput via instrument selection and optimisation Flexibility and versatility Automation System control or modular intelligence Range of autosamplers to maximise laboratory time Confidence System reliability, validation and ISO accreditation Fully self - diagnostic Qualified service and technical support Efficiency Sensitivity, resolution, reproducibility, linearity 6
7 quirements 150mm ACTUAL SIZE The solution 7
8 MX / High pressure mixers Building b UV-2070/2075 UV/Vis detectors FP-2020 Fluo detector PU-2080 Pump Up to 3 pump ternary gradient PU-2089 Pump Isocratic PC system including and JASCO - BOR LG / Low pressure mixers DG / In-line degassers Quaternary gradient One pump ternary/quaternary gradient LV Solvent switching valve PU-2085 Micro pump 8 Solvent switching system
9 etter systems resence MD-2010/2015 Diode Array detectors CD-2095 Circular Dichroism detector OR-2090 Chiral detector LC-Net II control WIN acquisition UV-2077 Multi-wavelength detector RI-2031 Refractive Index detector CL-2027 Chemiluminescence detector AS-2059 Autosampler HV Column switching valve CO-2060/2065 Column ovens (chilled) AS-2055 Autosampler 9
10 Integrated data HSS-2000 New 32 bit software allows control via LC-Net II and full integration in JASCO- BORWIN from the Windows 2000 platform and integrated Oracle database. Data analysis Spectrum processing Instrument validation Full automation of HPLC system along with audit trail for GLP compliance HSS-Studio Designed to help LC-2000Plus users meet current regulatory requirements including system validation, GLP, GMP and ISO 9000 compliance. Oracle database records a log of important sample and instrument related parameters and events for each analysis. Instrument validation and functional errors are also recorded. Spectra Manager The HSS-2000 provides instrument validation performance criteria according to electronic Standard Operating Procedures (SOP). Results are compared with user defined criteria and printed out to accompany SOP documentation. Spectral analysis software. Enables collection of spectral data from any JASCO UV/Visible, fluorescence or CD detector. 10
11 control and analysis JASCO-BORWIN New 32 bit software package operating on both Windows JASCO-BORWIN PDA Complete data acquisition and processing from one to four HPLC systems. Integration, quantitative analysis and mathematical functions. Display of up to 20 chromatograms (overlays, stacked and 3D plots). Unlimited zoom feature to display small chromatogram details. Automated quantitative analysis of multiple sample runs. Diode Array detector control and data processing software. GPC Advanced graphical manipulation Peak purity assessment Full automation of system including MD-2010/2015. Real time monitoring of 3D spectra and chromatographic data. Choice of display modes: Contour, 3D plots, six chromatograms, ratio chromatogram and UV spectra. Quantitative analysis at optimum wavelength. Data analysis functions: spectral library search, automatic recognition of chromatographic / spectral peak maxima / minima and simultaneous integration of up to 6 chromatograms. Molecular Weight distribution software. This optional program enables JASCO-BORWIN users to perform enhanced molecular weight calculations from gel permeation chromatography. 11
12 delivery Wide range Intelligent HPLC pumps offering a wide flow range for all applications. Isocratic High pressure binary or ternary gradient Low pressure binary, ternary or quaternary gradient PU-2080 Analytical Versatile time based programming of high pressure binary and low pressure ternary gradient elution, flow rate and solvent selection. Flow range 1µl/min to 10ml/min with precision of ±0.1%. PU-2085 Micro Ideal for 1-2mm columns and low flow rate gradients for LC-MS flow range 1µl to 4ml/min. PU-2086 Semi-preparative Flow range to 20ml/min with precision of ±0.2%. PU-2087 Preparative Flow range to 50ml/min with precision of ±1% PU-2080i Inert For biological separations without metallic material contact. PU-2089 Quaternary low pressure gradient pump Dedicated system with built-in 4 line degasser and mixing module for stepwise, linear and exponential gradient applications. Flow range 1µl/min to 10ml/min with precision (RSD) of <0.1%. Pressure profile Baseline 50 µl/min micro performance 12
13 of solvent systems Excellent pump performance giving accurate and precise analytical results. The Slow Suction Quick Delivery system provides the most reliable and stable solvent delivery over a wide flow rate range using only 2 plungers and 2 check valves. Flow precision of ±0.1% is achievable. The pulse free flow assures maximum detector sensitivity. The pump head design also minimises maintenance requirements. SSQD profile giving pulse-free flow Delivery head Flow Profile Discharge Suction 0 Modifier head 360 Reproducible results given by SSQD system SSQD 1% step gradient profile 13
14 Versatile au Fully automatic intelligent sample injection system for increased productivity and analytical precision. AS-2059 Sampling flexibility with automatic detection of sample rack type. 192 well positions (2 x 96) or 384 microplates with microplate rack. 120 x 2ml vial capacity or 224 microvials when using 200, 300 or 600µl microvial rack. Zero sample loss, variable sample volume injection method. Reagent addition and dilution. Pre-column derivatisation. Fully GLP/GMP compliant. Optional electronic cooling and heating (TC-2059) is available. AS position unit offering highly reproducible results in partial or full loop modes of operation. The AS-2057 offers a built-in Peltier cooling and heating unit. Sample pre-treatment Air segmented sampling enables automatic mixing of up to 2 different solvents or derivatising agents with the sample. By automating the derivatisation process, the precision and reproducibility of complex pre-column analyses can be optimised. 14
15 tosamplers In both fixed loop and variable volume injection modes, excellent reproducibility is achieved. In 20µl fixed loop mode, less than 0.2% RSD was given with less than 0.01% sample contamination. Excellent linearity and reproducibility Resolution of injection volume µl (0.1 µl step) µl (1.0 µl step) Contamination: % Minimal carryover Trays and vials For full automation, exchangeable racks are available to take a range of vials: Standard 2ml Micro-volume 0.3ml Disposable Eppendorf tubes Multiple microtitre plates 15
16 UV/Visible d for sensi UV-2070/2075 UV/Visible Range nm (UV-2070) Range nm (UV-2075) Versatile programming of wavelength, response, range, autozero and spectral scanning, with 10 program files. Storage of 10 spectra and baseline from 'on the fly' scanning capability. Spectra Manager, supplied with HSS control software for data processing of spectra. Excellent baseline stability from nonabberation Czerny-Turner monochromator system. Lamp-off timer to extend lamp life. Range of cells A tapered flow cell design eliminates refractive index effects. Various flow cells are available for a wide range of applications: Micro Preparative Ultra high pressure for SFC, LC-MS PTFE for biochemical samples Bioinert for biochemical samples or ion chromatography 16
17 etectors tivity and stability UV-2077 Multi-wavelength Simultaneous monitoring of 4 different wavelengths with optional ratio output for peak purity. Range nm set at 1nm intervals. Low noise, stable baseline. Spectral acquisition without interrupting chromatography. Four wavelength monitor Peak purity ratio chromatogram Baseline stability 17
18 Wide range MD-2010/2015 Diode Array Contour plot 3D plot Highly sensitive detectors with a wide operating range: MD-2010: nm (1nm data interval) MD-2015: nm (1.5nm data interval) As part of the PC controlled LC-2000Plus system, the JASCO-BORWIN PDA software provides a wide range of information: 3D chromatograms, contour plots, UV/Visible spectra, 6 wavelength chromatograms, a ratio chromatogram, spectral library and search facility. Stand alone units give chromatograms at 3 different wavelengths, plus the ratio chromatogram for peak purity. 50 spectra storage memory. 10 program files, each with up to 64 steps for time programming, wavelength and range for 3 channels, wavelength of ratio chromatogram and scale, autozero, marker output and spectral measurement. Multiwavelength acquisition Spectral data 18
19 of detectors FP-2020 Fluorescence The most sensitive detector on the market today. Excitation and emission ranges of nm (to 900nm with optional PMT). Time programming of wavelength, response range and spectral scan providing peak measurement at optimum excitation and emission wavelengths. Rapid scan for on-flow spectral acquisition of excitation and emission spectra without interrupting chromatographic elution. Storage of 10 excitation and emission spectra. Range of cells A variety of flow cells are available for the FP-2020: Analytical Micro High sensitivity Chemiluminescence Inert Rectangular (10 x 10mm) cuvette holder for applications including excitation and emission wavelength optimisation Unparalleled sensitivity 19
20 Optical dete CD-2095 Circular Dichroism The world's first Circular Dichroism based detector for Chiral Chromatography Simultaneous CD, UV and g factor signals Ratio of CD and UV signals, the g factor, gives direct determination of optical isomer separation and purity Spectral scanning with Spectra Manager Up to 100 x more sensitivity than ORD. g factor UV (310nm) CD (310nm) OR-2090 Chiral Low noise and drift Hg/Xe lamp to cover wavelength range from UV to visible Patented flow cell for low dispersion Artifact free Auto aligning flow cell easily removed for cleaning Detection of optically active sugars 20
21 ctors CL-2027 Chemiluminescence Equipped with exclusive, JASCO design, flowcell used for sensitive detection of lipids, nucleotides, nitrogen oxides (NOX) and catecholamines. Catecholamine detection RI-2031 Refractive Index Semi-preparative flow rate to 50ml/min Low noise, stable baseline Automatic time programming of sensitivity range and reference cell purging. Temperature stabilisation from 10 C above ambient to 45 C. GPC analysis of polystyrene standards 21
22 Support mo Mixers JASCO supplies a range of mixers for low and high pressure gradient systems Low pressure mixers LG Maximum 64 step linear gradient for 3 solvents. LG is available for 4 solvent mixing. High pressure mixers MX solvent mixer with 2 mixing lines. The higher mixing capability offers a stable baseline when the mobile phase has UV absorbance and the detection wavelength is below 230nm. MX solvent dynamic mixer with optional semi-micro mixers. DG In-line degasser Eliminates dissolved gases from 3 solvent lines. DG is a 4 line degasser. 22
23 dules CO-2060/2065 Column ovens Intelligent modules Range -15 C below ambient to 80 C (CO-2060) Range 10 C above ambient to 80 C (CO-2065) Accuracy ± 0.1 C Accepts column lengths to 30cm HV Switching Valve A 2 position valve for use in flow line switching between columns and auto-injector. The valve position can be switched manually or automatically via a contact closure. LV Solvent Selector Valve A 6 solvent selector used with the PU-2080 HPLC pump. Biocompatible systems For applications where sample contact with metallic surfaces must be avoided, JASCO offers a series of biocompatible modules. PU-2080i HPLC pump UV-2075i UV/Vis detector AS-2059i Autosampler AS-2055i Autosampler AS-2057i Autosampler MX i Dynamic high pressure mixer 23
24 Products described herein are JASCO InternationalCO., LTD., 4-21, Sennin-cho 2-chome, Hachioji, Tokyo , Japan Tel: Fax: Internet: JASCO Incorporated, 8649 Commerce Drive, Easton, Maryland , U.S.A. Tel: Tel: Fax: Internet: designed and manufactured by ISO-9001-certified JASCO Corporation. JASCO Europe s.r.l., Via Confalonieri 25, Cremella (Co), Italy Tel: Fax: Internet: JASCO Labor-und Datentechnik GmbH Deutschland, Robert-Bosch-Strasse 11, Gross-Umstadt, Germany Tel: Fax: Internet: JASCO (UK) Ltd., 18 Oak Industrial Park, Chelmsford Road, Great Dunmow, Essex, CM6 1XN, U.K. Tel: Fax: Internet: JASCO Eastern Europe Information Office, H-1113, Karolina UT 29-31, Budapest, Hungary Tel: Fax: Serving the global marketplace JASCO France s.a.r.l., 60 Bd du Maréchal Juin, Nantes, France Tel: Fax: Internet: with Analytical Instrumentation JASCO Benelux B.V., Industrieweg AS Maarssen, Netherlands Tel: Fax: Internet: JASCO Analitica Spain S.L., Emiliano Barral 13-B, Madrid, Spain Tel: Fax: Internet: JASCO Corporation , Ishikawa-cho, Hachioji,Tokyo 192, Japan JASCO Scandinavia AB, Ågatan 32, Mölndal, Sweden Tel: Fax: Internet: http: // ISO 9001 Certified CFLC-0203
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