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12 NanoDrop One Viewer software NanoDrop One Website.
13 NanoDrop One Website
14 NanoDrop One Viewer software NanoDrop One Website Software System Update Update Update Software, Update Note OK Language
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19 Measure dsdna, ssdna or RNA Reported Results Settings Detection Limits Calculations
20 Before you begin... Nucleic Acids dsdna, ssdna or RNA Tip Blank Tip Measure. Measure End Experiment
21 Related Topics
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23 Related Topics Nucleic Acid Setup.
24 Optional user-defined baseline correction Related Topics Beer-Lambert Equation (solved for concentration) Note
25 Extinction Coefficients vs Factors sample pathlength f Factors Used dsdna ssdna RNA Custom Factor
26 Measured Values Note A260 absorbance A230 and A280 absorbance Sample Pathlength
27 Reported Values Nucleic acid concentration A260/A280 purity ratio A260/A230 purity ratio Note
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29 Measure Microarray Samples Reported Results Settings Detection Limits Calculations
30 Before you begin...
31 Microarray Nucleic Acids Tip Tip Blank Tip Measure. Measure End Experiment
32 Related Topics
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34 Related Topics Microarray Setup.
35 Tip
36 > Dye/Chrom. Editor > Dye/Chrom. Editor Settings
37 Add or remove a dye Add custom dye Name Done Unit Extinction Coefficient Wavelength Add Dye Edit custom dye
38 Save Dye Delete custom dye Related Topics Available Options for Factors dsdna ssdna RNA Oligo DNA Oligo RNA Custom Factor Tip Note
39 Measured Values A260 absorbance Note A280 absorbance Dye absorbance Dye correction
40 Sample Pathlength Reported Values Nucleic acid concentration A260/A280 purity ratio Dye1/Dye2 concentration Note Related Topics
41 Measure using Custom Factor Reported Results Settings Detection Limits Calculations
42 Before you begin... Custom Factor Nucleic Acids Tip Blank Tip Measure. Measure End Experiment
43 Related Topics
44 Related Topics
45 Custom Factor Setup. f = 1/( * b)) f b Optional user-defined baseline correction Related Topics
46 Related Topics
47 Measure Oligo DNA or RNA Reported Results Settings Detection Limits Calculations
48 Before you begin...
49 Oligo DNA Nucleic Acids Oligo RNA Tip Blank Tip Measure. Measure End Experiment Related Topics
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52 Related Topics
53 Oligo RNASetup). Oligo DNASetup (or Factor f = 1/( * b) f b
54 Molecular Weight Number of Bases Molar Ext. Coefficient (260 nm) %GC Tip Related Topics
55 Related Topics Extinction Coefficients for Oligonucleotides N = N 1 2 N 1 Tip
56 Measured Values A260 absorbance Note A230, A280 absorbance Sample Pathlength
57 Reported Values Nucleic acid concentration A260/A280 purity ratio A260/A230 purity ratio Note Note Related Topics
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59 Measure A280 Proteins Reported Results Settings Detection Limits Calculations
60 Before you begin...
61 Protein A280 Proteins Tip Blank Tip Measure Measure End Experiment
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63 Related Topics
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65 Related Topics Protein A280 Setup.
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67 Tip > Protein Editor Settings > Protein Editor
68 Add custom protein Name Done Description Molar Extinction Mass Extinction Mass Extinction
69 Molar Extinction OK Edit custom protein OK
70 Delete custom protein Related Topics Related Topics
71 Beer-Lambert Equation (solved for concentration) Note Extinction Coefficients for Proteins Tip
72 Available Options for Extinction Coefficient 1 Abs = 1 mg/ml BSA IgG Lysozyme Tip Other protein Other protein Note Published Extinction Coefficients Tip Conversions Between molar and 1%
73 Conversions Between g/100 ml and mg/ml Tip Measured Values A280 absorbance Note A260 absorbance Sample Pathlength
74 Reported Values Protein concentration. A260/A280 purity ratio Note
75 Measure Labeled Proteins Reported Results Settings Detection Limits Calculations
76 Before you begin...
77 Protein & Labels Proteins Tip Tip Blank Tip Measure Measure End Experiment
78 Related Topics
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80 Reported values for Proteins & Labels application Related Topics Proteins & Labels Setup.
81 Tip Tip
82 Related Topics Related Topics
83 Available Options for Extinction Coefficient 1 Abs = 1 mg/ml BSA IgG Lysozyme Other protein Other protein Tip Note Measured Values A280 absorbance Note
84 Dye absorbance Dye correction Sample Pathlength Reported Values Protein concentration. Dye1/Dye2 concentration Related Topics
85 Measure A205 Proteins Reported Results Settings Detection Limits Calculations
86 Before you begin... Proteins Protein A205 Tip Blank Tip Measure Measure End Experiment Related Topics
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89 Related Topics Protein A205 Setup.
90 Related Topics Tip
91 Available Options for Extinction Coefficient 31 Scopes Other protein Note Tip Measured Values A205 absorbance Note A280 absorbance
92 Sample Pathlength Reported Values Protein concentration. Related Topics
93 Measure Total Protein Reported Results Settings Detection Limits
94 All reference and standards solutions First standard Concentration range of the standards requires one reference measurement Concentration values for standards up to 7 standards
95 Measure the reference and all standards blank the instrument
96 Swipe left one screen
97 Swipe left one screen Press and hold
98 Load more standards Run samples [application name] Setup Done [application name] Setup Done
99 Valid Curve indicator Before you begin... Proteins Protein BCA Tip Curve Type
100 Tip Blank Measure Measure Done Measure End Experiment Related Topics
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105 Related Topics
106 Protein BCA Setup. Linear Interpolation 2 nd order polynomial 3 rd order polynomial Note Note Related Topics
107 Measure Total Protein Reported Results Settings Detection Limits
108 Work quickly and do not allow prepared standards or samples to sit longer than necessary Measure standards and samples in triplicate
109 Before you begin... Proteins Protein Bradford Tip Curve Type Replicates
110 Tip Blank Measure Measure Done Measure End Experiment Related Topics
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115 Related Topics Protein Bradford Setup.
116 Linear Interpolation 2 nd order polynomial 3 rd order polynomial Note Note Related Topics
117 Measure Total Protein Reported Results Settings Detection Limits
118 Before you begin... Proteins Protein Lowry Tip Curve Type
119 Tip Blank Measure Measure Done Measure End Experiment Related Topics
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123 Related Topics
124 Protein Lowry Setup. Linear Interpolation 2 nd order polynomial 3 rd order polynomial Note Note Related Topics
125 Measure Total Protein Reported Results Settings Detection Limits
126 Before you begin...
127 Proteins Protein Pierce 660 Tip Curve Type
128 Tip Blank Measure Measure Done Measure End Experiment Related Topics
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132 Related Topics
133 Protein Pierce 660 Setup. Linear Interpolation 2 nd order polynomial 3 rd order polynomial Note Note Related Topics
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135 Measure OD600 Reported Results Settings Calculations
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137 Before you begin...
138 OD600 OD600 Tip Blank Tip Measure Measure End Experiment Related Topics
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140 Related Topics OD600 Setup.
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142 Related Topics Tip
143 Measured Values A600 absorbance Note A absorbance Sample Pathlength Reported Values Cell concentration.
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145 Measure Custom Method Delete Custom Method Reported Results
146 Load custom methods onto the instrument Custom Custom Load Method Load
147 Before you begin... Custom Custom Local Direct-Connect PC computer s assigned name
148 OK Local Direct-Connect PC computer name Run Method
149 Related Topics Custom Custom
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151 Related Topics
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153 Measure UV-Vis Reported Results Settings Detection Limits
154 Before you begin... Custom UV-Vis Tip Blank Tip Measure Measure. End Experiment
155 Related Topics
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157 Related Topics
158 Setup. UV-Vis User-defined wavelengths to be measured and reported at run time Note Optional automated pathlength selection Note Optional user-defined baseline correction Related Topics
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161 Measure Kinetics Create Kinetics Method Edit Kinetics Method Reported Results Settings Detection Limits
162 Kinetics Kinetics method name Save Method Create Method method name Edit Method > Settings Note Run Method Blank
163 Heat Cuvette to 37 C Note Measure Heat Cuvette to 37 C Note Pause Stop End Experiment Related Topics
164 Create Method Kinetics Kinetics Method Name Description Measurement range Wavelengths to monitor
165 Number of stages Time unit # intervals Interval times Delays rows boxes Save Method Run Method Related Topics
166 Kinetics Kinetics Edit Method Save Method Run Method Related Topics
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169 Show Delta Absorbance Vs Time
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171 Related Topics
172 Kinetics > Kinetics Create Method Edit Method. Kinetics Setup. Enter a name description Select the spectral range Note Enter up to 3 wavelengths Note Specify up to 5 stages Note
173 unit Delay Interval Time Note #Intervals Note #Intervals Duration rows boxes
174 Related Topics Note
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177 Note Absorbance = log intensitysample intensityblank A = b c Related Topics
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179 Set up Bluetooth connections on the instrument Settings System Bluetooth ( Off add a Bluetooth device Search For Devices
180 tap its name complete any instructions
181 Back Done Deselect Bluetooth input device System Bluetooth Profiles
182 Use For Input Paired Bluetooth Device Back Done Disconnect Bluetooth device System Bluetooth
183 Unpair Back Done Set up Ethernet connection
184 Select Wi-Fi network on the instrument System Wi-Fi ( Connect
185 Back Done
186 Configure Wi-Fi data storage on the remote computer NanoDrop One Viewer File > Set Up Wi-Fi Data Storage Instrument IP address Instrument Name Status Settings > System PC Name
187 Enabled Add OK Delete
188 Connectivity Status Local Direct-Connect PC* computer s assigned name* *
189 Connect ( OK )
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191 Show connectivity status OK Related Topics
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194 Cleaning pedestals for daily operation
195 Pipetting Samples
196 Data Storage tap the icon
197 Blank Tip Measure
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199 Related Topics Experiment Name Done
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202 General. Use Cuvette. Pathlength Done. Blank
203 Related Topics Measure.
204 Tip Tip
205 For best results: Problems associated with blanking
206 Solutions for blanking problems Supplies needed
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208 Blank Measure End Experiment Related Topics
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211 application name Application tab
212 * *
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214 Related Topics
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216 Edit default sample base name Sample Name Done Edit sample name swipe left Sample Name sample name
217 Done
218 Blank Measure Measure
219 Auto-Measure On Off Auto-Blank On Off End Experiment End Experiment
220 sample row
221 Related Topics
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223 Open instrument database of measurement results
224 Search Select Export selected experiments row Select
225 Export Export OK Select Delete selected experiments row Select Delete OK
226 Open an experiment row experiment name
227 Spectral data
228 Data Table Menu
229 Related Topics Label new experiment when you save it Add Identifier Done End Experiment
230 Label experiment in Data Viewer Manage Identifiers Add Identifier Done OK View assigned labels for an experiment Find labeled experiments Search OK Remove a label Manage Identifiers OK
231 Export data at end of experiment
232 Export OK End Experiment Export data from Data Viewer row Select Export
233 Export OK Delete data from any measurement screen Delete data from Data Viewer row
234 Print data from any measurement screen Print OK Print data from Data Viewer row experiment name Print OK
235 Print sample details OK Related Topics
236 View or change instrument settings Settings Wi-Fi Bluetooth Language Notice
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