Improved Methods for. Forensic Toxicology Analysis

Transcription

1 Improved Methods for Forensic Toxicology Analysis

2 Specialized Products for Forensic Toxicology The screening and confirmation for drugs of abuse and their metabolites present many challenges in toxicological analysis. Searching for illegal drugs in complex matrices, such as urine and blood, can prove to be difficult because if present, they may be found at very low concentration levels. Direct analysis of these samples is often hampered by endogenous compounds in the biomatrix. These interferences are often in higher concentrations than that of the target analytes and thus may mask their presence. We at Phenomenex understand your needs and have therefore developed products that allow you to get your work done with confidence. Phenomenex SPE, GC/MS, HPLC, and LC/MS products have the phases and formats you need in order to detect drugs of abuse from various matrices. We use the latest technology to ensure our products will give you the highest sensitivity and fastest analysis time every time.

3 Table of Contents Specialized Products for Forensic Toxicology-----pp. -6 Sample Preparation/SPE GC/MS LC/MS Applications pp. 8-5 PCP Methadone THC Cocaine Amphetamines piates Steroids Benzodiazepines Barbiturates MAM Blood Alcohols rdering Information pp. 8- Sample Preparation/SPE Sorbents GC Columns General GC Accessories HPLC Columns Key Premier Solutions: Products providing the optimal results for the analysis Standard Solutions: Products for general routine work Low Cost Solutions: Alternative products for labs interested in cost savings 009 Phenomenex, Inc. All rights reserved.

4 p. Specialized products Sample Preparation /SPE Effective Extraction and High Recovery Low Cost Solutions Strata Silica-Based SPE Sorbents Consistent manufacturing for accurate and reproducible results Inert material provides clean extracts Standing orders available Strata Screen-C Mixed-mode Cation Exchange Extraction of hydrophobic and basic drugs from biological matrices such as blood, serum, and urine Removes anionic and neutral interferences without seriously affecting the recovery of the basic analyte Alternative for: Varian Bond Elut Certify I, UCT Clean Screen DAU, JT BAKER Bakerbond arc -, IST ISLUTE HCX, Macherey-agel Chromabond Drug Strata Screen-C GF Improved Gravity Flow Large particle for clog-free, superior flow in absence of a vacuum Good for viscous samples such as horse urine and plasma, or gravity flow applications High extraction efficiency compared to traditional mixed-mode sorbents of smaller particle size Alternative for: Varian Bond Elut Certify HF, UCT XtrackT DAU Contact Phenomenex or your local Phenomenex distributor for Strata Screen-C GF ordering information.

5 p. Sample Preparation /SPE Specialized products Premier Solutions Strata -X Polymer-Based SPE Sorbents Lot-to-lot reproducibility provides consistent recoveries from extraction-to-extraction ph stability from -4 for safe and stable basic elution of many drug compounds High analyte capacity for loading up to 66 % more in comparison to silica-based sorbents per gram Strata-X is de-condition resistant for higher recovery, precision, & ruggedness % Recovery Drying Time (min) Strata-X C8 Strata-X-C Strong Cation Exchange and Reversed Phase Withstands high organic washes to more completely remove matrix contaminants Attain better LDs/LQs for increased sensitivity, accuracy, and precision of data ph limit -4 offers more controlled selectivity and higher recoveries of analytes Eliminate the need for harsh halogenated solvents Strata-XL-C Improved Gravity Flow Large particle size provides excellent flow for viscous biological fluids such as horse urine and plasma High recovery of drugs using the same selectivity as Strata-X-C Consistent tube-to-tube flow An alternative for: Waters asis MCX, UCT s StyreScreen DBX Strata-X Reversed Phase Controlled selectivity for target analytes for removal of contaminants and better elimination of matrix effects High analyte capacity for loading up to 66 % more in comparison to silica-based sorbents per gram Withstands aggressive organic washes ( % organic)* without breakthrough of analyte Reproducible recoveries from extraction-to-extraction and superior lot-to-lot reproducibility Strata-XL Improved Gravity Flow Large particle size provides excellent flow for viscous biological fluids such as horse urine and plasma High recovery of drugs using the same selectivity as Strata-X Consistent tube-to-tube flow An alternative for: Waters asis HLB, Varian EXUS, FCUS and PLEXA, UCT s StyreScreen DVB, JT Baker H0-pilic DVB, IST EV+, Macherey-agel s Easy and HR-X *Please see T-007, "Testosterone on Strata-X vs. C8-E" For Strata-X on-line extraction cartridges, see p. 8.

6 p. 4 Specialized products GC/MS EW GC Column Dimensions for Fast Drug Analysis Standard Solution EW Zebron GC Columns Developed for Forensic Testing Phenomenex now offers new optimized column dimensions (0 meter x 0.8 mm x 0.8 µm) in our award winning Zebron GC column phases. This special dimension allows for a major improvement in sample throughput, while still providing great peak shapes and resolution. In addition, the extended temperature stability range and the low column bleed levels allow for high temperature removal of matrix contamination and fast elution of target analytes. Decrease run time by at least 0 % Symmetrical peak shapes and great resolution for drugs of abuse Better limits of detection (LD) and quantitation (LQ) ew ptimized Column Dimensions Available in These Popular Phases: Zebron ZB-5MSi - Specially deactivated for analysis of ZB-5ms - Provides improved resolution for aromatic derivatized and un-derivatized drug compounds. compounds Alternative for: Alternative for: DB-5, HP-5msi, Rtx-5ms, Rtx-5Amine, HP-5ms DB-5ms, DB-5ms EVDX, VF-5ms, CP-Sil 8 CB MS, DB-5.65 ZB-ms - Good low polarity phase for general drug screening. Alternative for: DB-ms, HP-ms, Rtx-ms, VF-ms, MD-, Equity-, AT-ms ZB-XLB - unique phase that can be used to improve resolution of structurally similar compounds Alternative for: DB-XLB, Rtx-XLB, MD-, VF-Xms App ID 790 Reduce analysis time! Traditional Dimension 790, Column: Zebron ZB-5MSi Dimensions: 5 meter x 0.5 mm x 0.5 μm Part o.: 7EG-G08- Injection: Split 60 C, μl Carrier Gas: ml/min (constant flow ) ven Program: 50 C to 0 5 C/min Detector: 0 C Sample: ppm BSTFA. Carboxy-THC-d. Carboxy-THC App ID 779 ptimized Dimension 0 % Faster Run Time, Column: Zebron ZB-5MSi Dimensions: 0 meter x 0.8 mm x 0.8 μm Part o.: 7CD-G08-08 Injection: Split 80 C, μl Carrier Gas: 0.7 ml/min (constant flow ) ven Program: 00 C to 00 0 C/min Detector: 0 C Sample: ppm BSTFA. Carboxy-THC-d. Carboxy-THC min min

7 p. 5 EW GC Columns Designed for Drugs of Abuse GC/MS Specialized products Premier Solution EW Zebron ZB-Drug- is a Specialized Solution Developed for Forensic Testing Zebron ZB-Drug- is a new mid polarity phase designed to provide fast analysis for compounds in drugs of abuse. The unique stationary phase improves separation of target analytes from common matrix interferences present in biological samples. The special deactivation procedure improves peak shape for acidic and basic drugs, allowing for more accurate quantitation. MS certified bleed levels allow for low level detection. Specially deactivated to improve quantitation for drug compounds ew 0 meter x 0.8 mm x 0.8 µm dimensions significantly reduces run time Temperature Limits (-60 to 0/40 C) Zebron ZB-Drug-: Faster Results, Better Peak Shape, Great Separation App ID 67 piates on a ZB-5ms,,4 Column: Zebron ZB-5ms Dimensions: 0 meter x 0.8 mm x 0.8 μm Part o.: 7CD-G08-08-C Injection: Split 40 C, μl Carrier Gas: ml/min (constant flow ) ven Program: 40 C to 0 0 C/min for min Detector: 0 C Sample: 0 ppm BSTFA/Pyridine. Codeine-d6. Codeine. Morphine-d 4. Morphine App ID 7806 Unique Selectivity for Drugs piates on a ZB-Drug-,4 Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 μm Part o.: 7CD-G0-08 Injection: Split 80 C, μl Carrier Gas: 55 cm/sec (constant flow ) ven Program: 80 C to 40 0 C/min Detector: 0 C Sample: 0 ppm BSTFA/Pyridine. Codeine-d6. Codeine. Morphine-d 4. Morphine min min

8 p. 6 Specialized products LC/MS HPLC Columns for LC/MS Analysis Premier Solutions Phenomenex HPLC Columns Phenomenex s LC/MS columns are designed to give high sensitivity, optimal retention, and a full range of selectivities for drugs of abuse. ur columns have low MS bleed, which results in less background noise. This is important when quantitating samples in low concentration. Gemini Achieve high sensitivity, optimal retention, and great separation of basic drugs Positively impact laboratory productivity with longer column lifetime Extended ph stability - Luna Synergi MercuryMS High quality phases good for all types of forensic samples Low MS bleed improves sensitivity for LC/MS/MS methods Extensive batch traceability and reproducibility data supplied with every column Unique phases that provide enhanced selectivity across the polarity scale ften resolves compounds that co-elute on traditional column chemistries Synergi Hydro-RP (C8 with Polar Endcapping) has maximum hydrophobic selectivity with added polar retention. Also compatible with 00 % aqueous conditions Ultra-fast, low-cost analysis for high-throughput LC/MS laboratories Packed with Luna, Synergi, and Gemini material Short 0 and 0 mm cartridge formats use a new proprietary slurry packing process App ID 794 Cocaine and Metabolites 4 Column: Luna µm HILIC Dimensions: 00 x.0 mm Part o.: 00D-4449-B0 Mobile Phase: A: Acetonitrile/ 00 mm Ammonium formate, ph. (50:45:5) B: Acetonitrile/ Water/ 00 mm Ammonium formate ph. (50:45:5) Gradient: A/B (00:0) for.7 min, to A/B (45:55) in. min, hold for.7 min, then to A/B (00:0) for 6 min Flow Rate: 0.4 ml/min Detection: Mass Spectrometer (MS) Sample:. Cocaine. orcocaine. Ecgonin Methyl Ester 4. Benzoylecgonine 5 5. Ecgonine min

9 Applications

10 p. 8 Applications PCP PCP Extraction of Phencyclidine from Urine and Analysis by GC/MS Phencyclidine (PCP) induces hallucinogenic and neurotoxic effects. Since a vast majority of the parent drug and its metabolite are excreted in urine, abuse is determined by detecting the parent drug. SPE GC/MS Solutions Premier Solution: Strata -X-C Low Cost Solution: Strata Screen-C PCP is a weakly basic compound with a pk a of about 8.5, but a relatively high LogP of 5.. The pk a and high LogP allow it to be effectively retained through hydrophobic and ionic interactions on mixed-mode cation-exchange sorbent such as Screen-C or Strata-X-C. The high hydrophobic retention of PCP can reduce recoveries if strong organic elution is not used. Premier Solution: Zebron ZB-Drug- Standard Solution: Zebron ZB-5MSi Derivatization of this compound is not necessary as the amine functionality does not display adverse secondary interactions in the GC system. The single peak is easily analyzed with good sensitivity. GC/MS Cut off Derivatization 5 ng/ml n/a PCP App ID 7800 Mass Ion(s) Quant Ion Secondary Ion Tertiary Ion PCP PCP - d5 (IS) Phencyclidine on a Zebron ZB-Drug-, Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G0-08 Injection: Split 80 C, 0.5 µl Carrier Gas: 55 cm/sec (constant flow) ven Program: 60 C to 40 0 C/min Detection: 0 C Sample: Analytes at ppm in Ethyl acetate. Phencyclidine-d5. Phencyclidine Recovery of PCP by SPE min % Recovery App ID 7787 Phencyclidine on a Zebron ZB-5MSi Column: Zebron ZB-5MSi Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G08-08 Injection: Split 60 C, µl Carrier Gas: 0.6 ml/min (constant flow) ven Program: 50 C to 0 0 C/min Detection: 0 C Sample: Analytes at ppm in Ethyl acetate. Phencyclidine-d5. Phencyclidine, Strata-X-C SPE Method Sorbent: Strata-X-C, 60 mg/ ml Part o.: 8B-S09-UBJ Condition: ml methanol followed by ml water Load: 4 ml ( ml of analyte and IS spiked human urine acidified with % H P 4 ; diluted with ml 00 mm KH P 4, ph 6.0) sample Wash : ml 0. HCI Wash : ml methanol Dry: min under 0 in. Hg Elute: ml 5 % H 4 H/Methanol ( x ml) Evaporate: Under nitrogen Reconstitute: 00 µl Ethyl acetate min

11 p. 9 Methadone Extraction of Methadone from Urine and Analysis by GC/MS Methadone binds with opiate receptors and displays many of the same physiological affects as heroin. However, it is not structurally related to heroin or other opiate compounds. To determine abuse, a separate screening method must be performed. SPE GC/MS Solutions Me t h a d o n e Premier Solution: Strata -X-C Low Cost Solution: Strata Screen-C Methadone is a non-polar basic compound that is well retained on cation-exchangers, such as Strata Screen-C and Strata X-C, or on neutral sorbents such as Strata-X. Premier Solution: Zebron ZB-Drug- Standard Solution: Zebron ZB-5MSi Methadone can be analyzed by GC/MS, similar to other drugs. Derivatization improves peak shape by decreasing reactivity with system components. Applications App ID 7804 GC/MS Cut off Derivatization 00 ng/ml n/a Mass Ion(s) Quant Ion Secondary Ion Tertiary Ion Methadone-d9 (IS) 78 0 Methadone 7 94 Methadone on a Zebron ZB-Drug- Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G0-08 Injection: Split 60 C, µl Carrier Gas: 55 cm/sec (constant flow) ven Program: 00 C to 00 5 C/min Detection: 0 C Sample: 4 ppm in Ethyl acetate. Methadone-d9. Methadone Methadone Recovery of Methadone by SPE min % Recovery App ID 7790 Methadone on a Zebron ZB-5MSi 790 Column: Zebron ZB-5MSi Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G08-08 Injection: Split 0:@ 80 C, µl Carrier Gas: 0.7 ml/min (constant flow) ven Program: 00 C to 00 0 C/min Detection: 0 C Sample: 4 ppm in Ethyl acetate. Methadone-d9. Methadone Strata-X-C SPE Method Sorbent: Strata-X-C, 60 mg/ ml Part o.: 8B-S09-UBJ Condition: ml methanol followed by ml water Load: ml urine spiked with analytes and IS (0.5 µg/ml); dilute : with 00 mm KH P 4 (ph 6); acidified with % phosphoric acid Wash : ml 0. HCI Wash : ml methanol Elute: ml 5 % H 4 H/Methanol ( x ml) Evaporate: Under nitrogen Reconstitute: 00 µl Ethyl acetate min Please inquire about other applications.

12 p. 0 Applications THC THC Extraction of 9 Carboxy-THC from Urine and Analysis by LC/MS or GC/MS Tetrahydrocannabinol (THC), the main active chemical in marijuana, is mainly metabolized to 9-carboxy--or- 9 -carboxy-thc (THC-CH). This compound is then conjugated with glucuronic acid and excreted in urine. SPE GC/MS LC/MS Solutions Premier Solution: Strata -X-C Low Cost Solution: Strata Screen-A or -C Though THC is best extracted using mixed-mode anion-exchange sorbents, such as Strata Screen-A, for convenience many labs will use a mixed-mode cation-exchange sorbent since they are already using this type of sorbent for the majority of their other methods. THC-CH and THC are effectively extracted from Strata-X-C and Screen-C through disruption of hydrophobicity and hydrogen bonding mechanisms. Removal of more polar matrix interferences can still be effectively done by washing with an organic solution acidified at or below ph. Premier Solution: Zebron ZB-Drug- Standard Solution: Zebron ZB-5MSi In many countries, THC is the most commonly screened drug. Due to the high volume of samples, system stability and fast run time are critical. Starting the GC oven at >00 o C did not affect peak shape or quantitation of THC-CH, but allowed for much faster cycle times significantly improving sample throughput. Premier Solution: Gemini C8 Acidic compounds, such as THC-CH are often run under mildly basic conditions in negative ion mode. Gemini allows the user an extended ph range to increase basic conditions much further, and create more favorable mass spec ionization for acids and neutrals in a mixture together. In this application, at ph 0, both the THC neutral parent and the THC-CH metabolite are successfully de-protonated for optimum MS sensitivity in negative ion mode.. THC-CH H H H Recovery of THC by SPE % Recovery Strata-X-C 9 Strata Screen-C H SPE Method Sorbent: Strata-X-C, 60 mg/ ml Part o.: 8B-S09-UBJ Condition: ml methanol followed by ml 0. HCl Load: Hydrolyzed urine sample Base hydrolysis: ml urine spiked with analyte and IS, add 00 µl 0 KH. Mix, vortex and hydrolyze for 0 min at 60 C. Cool and adjust ph to (by adding 00 µl glacial acetic acid) Wash : ml 0. HCl Wash : ml 0. HCl/acetonitrile (70:0) Dry: 4 min under vacuum. To further remove water: add 00 µl glacial acetic acid, letting it percolate under gravity for 0-40 sec. Dry sorbent for min under vacuum. Elute: ml % glacial acetic acid/acetonitrile ( x ml) Dry: Blow down to dryness 40 C Reconstitute: Add 0 µl 0 mm imidazole/ethylacetate + 0 µl BSTFA ( % TMCS). React at 70 C for 0 min. SPE Method Sorbent: Strata Screen-C, 50 mg/ ml Part o.: 8B-S06-SBJ Condition: ml methanol followed by ml 50 mm phosphoric acid Load: Hydrolyzed urine sample Base hydrolysis: ml urine spiked with analyte and IS, add 00 µl 0 KH. Mix, vortex and hydrolyze for 0 min at 60 ºC. Cool and adjust ph to 4-5 (by adding 65 µl glacial acetic acid followed by addition of ml 0. M sodium acetate (ph 7)/methanol (95:5) Wash : ml 50 mm phosphoric acid Wash : ml 50 mm phosphoric acid/methanol (80:0, v:v) Dry: min under vacuum; add 0.5 ml hexane followed by additional drying for min Elute: ml hexane/ethyl acetate (80:0, v:v) Evaporate: 40 ºC Reconstitute: Add 5 µl ethyl acetate and 5 µl BSTFA ( % TMCS). 70 ºC for 0 min. H

13 p. App ID 790 THC and Metabolite on Gemini C8 Column: Gemini µm C8 Dimensions: 50 x.0 mm Part o.: 00B-449-Y0 Mobile Phase: A: 0 mm H 4 HC (ph 0) B: Acetonitrile Gradient: A/B (85:5) to A/B (5:95) in.9 min, hold 0 sec, then to A/B (85:5) hold for 0 sec Flow Rate: 0.8 ml/min Detection: 450 C Sample:. -nor-9-carboxy-thc (THC-CH). -9-tetrahydrocannabinol (THC)-d. -9-tetrahydrocannabinol (THC) GC/MS Cut off 5 ng/ml Derivatization BSTFA (with % TMCS) Mass Ion(s) Quant Ion Secondary Ion Tertiary Ion Carboxy- 9 -THC THC Applications D-Carboxy- 9 -THC (IS) Carboxy-THC on a Zebron ZB-Drug min App ID 780 Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G0-08 Injection: Split 80 C, 0. µl Carrier Gas: 55 cm/sec (constant flow) ven Program: 0 C to 40 0 C/min Detection: 0 C Sample: Analytes at.5 ppm in BSTFA. Carboxy-THC-d. Carboxy-THC, H min H App ID 779 Carboxy-THC on a Zebron ZB-5MSi 779 Column: Zebron ZB-5MSi Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G08-08 Injection: Split 80 C, µl Carrier Gas: 0.7 ml/min (constant flow) ven Program: 00 C to 00 0 C/min Detection: 0 C Sample: Analytes at. ppm in BSTFA. Carboxy-THC-d. Carboxy-THC, min Please inquire about other applications.

14 p. Applications Co c a i n e Cocaine Extraction of Benzoylecgonine from Urine and Analysis by LC or GC/MS Cocaine is extensively metabolized by the liver, with only % being excreted in the urine unchanged. Abuse is thus detected by monitoring its main metabolite, benzoylecgonine. SPE GC/MS LC/MS Solutions Premier Solution: Strata -X or Strata-X-C Low Cost Solution: Strata or Strata Screen-C Benzoylecgonine contains both an amine and carboxylic acid functionality allowing it to be retained through multiple mechanisms. Typically, benzoylecgonine is extracted using a mixed-mode cation-exchanger such as Strata Screen-C or Strata X-C. However, the ammonium hydroxide used for elution on cation-exchangers causes hydrolysis of the methyl ester in cocaine and cocaethylene converting them into carboxylic acid containing benzoylecgonine metabolite. Using a neutral sorbent such as Strata-X does not require high ph elution, allowing for independent analysis of all three cocaine compounds. Premier Solution: Zebron ZB-Drug- Standard Solution: Zebron ZB-5MSi The single compound of benzoylecgonine eluted in just over minutes by using a fast temperature ramp. To ensure all matrix contamination is removed, a final oven temperature of 0 o C was used. verall cycle time is kept to a minimum due to the higher starting oven conditions, which reduce the need for GC cool down time. Premier Solution: Luna HILIC Using a HILIC (Hydrophilic-Interaction-Liquid-Chromatography) LC/MS column, cocaine and its metabolites will elute in less than 7 minutes. Adequate separation is achieved for the key metabolites for further detection by MS. Recovery of Cocaine by SPE % Recovery App ID 794 Cocaine and Metabolites Column: Luna µm HILIC Dimensions: 00 x.0 mm Part o.: 00D-4449-B0 Mobile Phase: A: Acetonitrile/ 00 mm Ammonium formate, ph. (50:45:5) B: Acetonitrile/ Water/ 00 mm Ammonium formate ph. (50:45:5) Gradient: A/B (00:0) for.7 min, to A/B (45:55) in. min, hold for.7 min, then to A/B (00:0) for 6 min Flow Rate: 0.4 ml/min Detection: Mass Spectrometer (MS) Sample:. Cocaine. orcocaine. Ecgonin Methyl Ester 4. Benzoylecgonine 5. Ecgonine min Benzoylecgonine Cocaine Cocaethylene Benzoylecgonine Cocaine Cocaethylene Benzoylecgonine Cocaine Cocaethylene Strata-X Strata-X-C Strata Screen-C SPE Method Sorbent: Strata-X, 0 mg/ ml Part o.: 8B-S00-TAK Condition: ml methanol / ml water Load: ml spiked Porcine plasma acidified with % H P 4 (Diluted : with water) Wash: ml 0 % methanol in water Dry: min at 0 in. of Hg Elute: ml methanol Evaporate: Under 40 o C Reconstitute: Depends on analysis technique SPE Method Sorbent: Strata Screen-C, 50 mg/ ml Part o.: 8B-S06-SBJ Condition: ml methanol followed by ml water Load: ml porcine plasma spiked with analytes diluted : with water Wash : ml 0. HCl Wash : ml methanol Dry: min under vacuum Elution: 5 % ammonium hydroxide in methanol Evaporate: Under 40 ºC Reconstitute: Depends on analysis technique

15 p. Benzoylecgonine Co c a i n e Applications H GC/MS Cut off Derivatization 50 ng/ml BSTFA (with % TMCS) Mass Ion(s) Quant Ion Secondary Ion Tertiary Ion D-Benzoylecgonine (IS) Benzoylecgonine Cocaethylene Benzoylecgonine on a Zebron ZB-Drug- App ID 7805 Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G0-08 Injection: Split 60 C, µl Carrier Gas: 55 cm/sec (constant flow) ven Program: 5 C to 0 5 C/min Detection: 0 C Sample: Analytes at 4 ppm in BSTFA. Benzoylecgonine-d,. Benzoylecgonine Cocaine min Benzoylecgonine on a Zebron ZB-5MSi App ID Column: Zebron ZB-5MSi Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G08-08 Injection: Split 50 C, µl Carrier Gas: 0.7 ml/min (constant flow) ven Program: 60 C to 0 0 C/min Detection: 0 C, Sample: Analytes at 4 ppm in BSTFA. Benzoylecgonine-d. Benzoylecgonine min Please inquire about other applications.

16 p. 4 Applications Am p h e ta m i n e s Amphetamines Extraction of Amphetamine from Urine and Analysis by LC/MS or GC/MS Amphetamines are very addictive stimulant drugs that affect the central nervous system. Methamphetamines are metabolized to amphetamines so abuse is typically detected by monitoring both compounds initially by immunoassay. A positive result is then confirmed by LC/MS or GC/MS. SPE GC/MS LC/MS Solutions Premier Solution: Strata -X-C Low Cost Solution: Strata Screen-C Amphetamines are polar aromatic compounds that are basic in nature. This polarity makes them difficult to retain through hydrophobic interactions, but they are well-suited for cation-exchange sorbents such as Strata Screen-C or Strata-X-C. High organic washes remove a majority of hydrophobic matrix interferences that can build up on the GC columns. Premier Solution: Zebron ZB-Drug- Standard Solution: Zebron ZB-5MSi When compared to other drug classes, these compounds are more volatile and require a lower starting oven condition to achieve proper peak shape. However, by ramping quickly up to 0 o C matrix interferences can still be efficiently removed preventing system stability problems. It is also worth noting that separation is achieved between the analyte and its deuterated analog. This separation does not affect quantitation in any way. Premier Solution: Gemini C8 At high ph, basic compounds, such as amphetamines and methamphetamines, are neutralized. Gemini is ph stable from -, providing longer column lifetime when running at high ph. Absolute Recovery of Amphetamines by SPE % Recovery HMMA 66 MDMA 76 MDA 8 MDMA-DS 86 MDA-DS Strata-X-C 70 Amphetamines 65 Methamphetamines SPE Method Sorbent: Strata-X-C, 0 mg/ ml Part o.: 8B-S09-TAK Condition: ml methanol followed by ml water Load: ml porcine plasma spiked with analytes diluted : with water Wash : ml water Wash : ml 0. M acetic acid Wash : ml methanol Elution: ml % of ammonium hydroxide (8-0 %)* in methanol ( x 500 µl) Evaporate: To dryness under gentle nitrogen flow Reconstitute: Add 00 µl mobile phase ote: To prevent the loss of amphetamine and methamphetamine during solvent evaporation, add two drops of concencentrated HCl before the evaporation step Amphetamine H Sa m p l e Pr e pa r at i o n Tip Be f o r e b l o w i n g d o w n s a m p l e s, b e s u r e to m a k e t h e m i n t o t h e s a lt form by acidifying your solution. Fa i l u r e to d o s o c a n r e s u lt in s a m p l e l o s s d u e to e va p o r at i o n a n d r e s u lt i n g p r o b l e m s w i t h sample detection. Methamphetamine H *Most commercially available H 4 H is 8-0 % stock concentration

17 p. 5 App ID 7900 App ID 7809 GC/MS Cut off Derivatization 500 ng/ml HFBA Mass Ion(s) Quant Ion Secondary Ion Tertiary Ion D-Amphetamine (IS) 44 8 Amphetamine D4-Methamphetamine (IS) 6 Methamphetamine D5-MDA (IS) MDA D5-MDMA (IS) MDMA D5-MDEA (IS) MDEA Amphetamines in Urine on a Gemini C8 Column: Gemini 5 µm C8 Dimensions: 50 x.0 mm Part o.: 00F-445-Y0 Mobile Phase: 0 mm Ammonium Formate, 0. % formic acid / Acetonitrile (90:0) Flow Rate: 0.6 ml/min Detection: 450 C min Sample:. 4-Hydroxy--methoxymethamphetmine (HMMA). Amphetamine (AMPH). Methamphetamine (M-AMPH) 4.,4-Methylenedioxy-amphetamine (MDA)-d5 5.,4-Methylenedioxy-amphetamine (MDA) 6.,4-Methylendiox-methamphetamine (MDMA)-d5 7.,4-Methylendiox-methamphetamine (MDMA) Amphetamines on a Zebron ZB-Drug- 6 7 Expanded Amphetamines on a Zebron ZB-Drug- Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G0-08 Injection: Split 80 C, 0. µl Carrier Gas: 55 cm/sec (constant flow) ven Program: 70 C to 0 0 C/min to 70 0 ºC Detection: 0 C Sample: Analytes are 40 ppm derivatized with HFBA. Amphetamine-d. Amphetamine. Methamphetamine-d4 4. Methamphetamine 5. MDA-d5 6. MDA 7. MDMA-d5 8. MDMA 9. MDEA-d5 0. MDEA H min App ID 7807 Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G0-08 Injection: Split 60 C, µl Carrier Gas: 55 cm/sec (constant flow) ven Program: 0 C to 0 5 C/min Detection: 0 C Sample: Analytes are 0 ppm derivatized with HFBA. Amphetamine-d. Amphetamine. Methamphetamine-d4 4. Methamphetamine min Am p h e ta m i n e s Applications Please inquire about other applications.

18 p. 6 Applications p i at e s piates Extraction of piates from Biological Matrices with High Recovery and Analysis by LC/MS or GC/MS There are a number of opiate related compounds known to have addictive properties. The standard structure contains five ring systems and is substituted at various positions with hydroxyls, ketones, or other groups that contribute to both its pain reducing and its addictive properties. Solutions Premier Solution: Strata -X-C SPE Low Cost Solution: Strata or Strata Screen-C The nitrogen containing ring system give opiates their basic property and allows them to be extracted using mixed-mode cation exchange sorbents. The ion-exchange retention mechanism allows for much more aggressive wash conditions (up to 00 % organic) for more effective removal of other co-extracted urine components. Premier Solution: Zebron ZB-Drug- Standard Solution: Zebron ZB-5MSi GC/MS If the patient has been using other opiate related compounds such as heroin or norcodeine, these compounds will also be co-extracted and can interfere with the analysis. In such cases, alternative GC/MS conditions should be developed to allow for accurate quantitation of these compounds. Premier Solution: Luna HILIC A typical SPE method elutes opiates in a high concentration of organic solvent. This concentration is stronger than what is typically used to begin a reversed phase LC method, thus requiring an additional dry-down step followed by reconstitution in the proper mobile phase concentrations before injection. Employing HILIC (Hydrophilic-Interaction-Liquid-Chromatography) is LC/MS an excellent complement to this extraction process, since this technique uses a starting mobile phase which employs a high concentration of organic solvent. Luna HILIC columns are ideally suited for the direct injection of eluted samples following SPE. Standard Solution: Synergi Hydro-RP Synergi Hyrdro-RP (C8) has an enhanced hydrophobic selectivity that provides extra retention for polar drugs. This results in baseline separation for all opiates. The column is also compatible with 00 % aqueous conditions. GC/MS Cut off 00 ng/ml Recovery of piates by SPE Derivatization BSTFA (with % TMCS) % Recovery Strata-X-C SPE Method Sorbent: Strata-X-C, 0 mg/ml Part o.: 8B-S09-TAK Condition: ml methanol followed by ml water Load: ml urine spiked with analytes and IS (0.8 µg/ml) Dilute : with phosphate buffer solution (ph 7) Acidified with % phosphoric acid Wash : ml 0. HCI Wash : ml methanol Elute: ml 5 % H 4 H/Methanol ( x 500 µl) Evaporate: Dry down under nitrogen Reconstitute: Add 00 µl water Lo o k i n g to r u n o p i at e s o n a s ta n d a r d C8 c o l u m n? Pl e a s e inquire w i t h yo u r H l o c a l Ph e n o m e n e x r e p r e s e n tat i v e. App ID 7806 App ID Mass Ion(s) Quant Ion Secondary Ion Tertiary Ion D6 Codeine (IS) Codeine D Morphine (IS) 4 47 Morphine piates on a Zebron ZB-Drug- H, min Column: Luna µm HILIC Dimensions: 00 x.0 mm Part o.: 00D-4449-B0 Mobile Phase: A: Acetonitrile / 00 mm Ammonium formate, ph. (90:0) B: Acetonitrile / 00 mm Ammonium formate, ph. (50:50) Gradient: A/B (00:0) for min to A/B (0:00) in min, hold for.5 min Flow Rate: 0.4 ml/min Detection: MS ote: Direct injection following Acetonitrile/ Water (5:) protein precipitation (PPT) Morphine and Metabolites on a Luna HILIC Sample:. Morphine. ormorphine. Morphine--ß-glucuronide Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G0-08 Injection: Split 80 C, µl Carrier Gas: 55 cm/sec (constant flow) ven Program: 80 C to 40 0 C/min Detection: 0 C Sample: Analytes are 4 ppm derivatized with BSTFA. Codeine-d6. Codeine. Morphine-d 4. Morphine min

19 p. 7 Healthcare piates Healthcare piates Extraction of Various Prescribed piates from Urine and Analysis by GC/MS Even though opiate related compounds pose the risk of addiction, their therapeutic benefit is important for pain management. piates such as hydrocodone (Vicodin), oxycodone (xycontin), and meperidine (Demerol) are routinely prescribed for pain control, but due to their addictive qualities, they often become the subject of abuse. SPE GC/MS GC/MS Cut off Derivatization Premier Solution: Strata -X-CW Low Cost Solution: Strata Screen-C Premier Solution: Zebron ZB-Drug- Standard Solution: Zebron ZB-5MSi To improve peak shape, opiates are often derivatized using BSTFA with % TMCS. When analyzing one or two opiates, this approach works well. However, some opiate structures form multiple products when single step derivatization is done. To ensure a single reaction product for hydromorphone or oxycodone, an oxime derivative is formed prior to reaction with BSTFA. The oxime reaction eliminates the multiple points of interaction resulting in a single derivative product. nce derivatization has been completed, GC/MS analysis can be performed. Separation of all closely related isomers can be somewhat challenging, requiring special attention to those compounds that share the same mass ions such as orcodeine and Morphine. 00 ng/ml BSTFA (with % TMCS) Solutions Applications App ID 6555 Mass Ion(s) Quant Ion Secondary Ion Tertiary Ion D-Morphine (IS) 4 47 Morphine D6-Codeine (IS) Codeine xymorphone D6-Hydromorphone (IS) Hydromorphone D6-xycodone (IS) xycodone Hydrocodone Healthcare piates on a Zebron ZB-5MSi Underivatized Column: Zebron ZB-5MSi Dimensions: 0 meter x 0.5 mm x 0.5 µm Part o.: 7HG-G08- Injection: Splitless 50 C, µl Carrier Gas: ml/min (constant flow) ven Program: 70 C for 0.5 min to 60 8 C/min Detection: 80 C Sample: Analytes at ppm in Methanol. Codeine 4 5. orcodeine. Morphine 4. Hydrocodone 5. Hydromorphone 6. xycodone 7. xymorphone min 6 H App ID 7899, H H Expanded piates on a Zebron ZB-Drug- Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G0-08 Injection: Split 60 C, µl Carrier Gas: 0.7 ml/min (constant flow) ven Program: 80 C to 40 0 C/min Detection: 0 C Sample: Analytes are 4 ppm derivatized with. Hydroxylamine. BSTFA 0. Morphine-d. Morphine. Codeine-d6 4. Codeine 5. xymorphone 9 6. Hydromorphone-d6 7. Hydromorphone 8. xycodone-d6 9. xycodone 8 0. Hydrocodone min H Codeine Please inquire about other applications.

20 p. 8 Applications St e r o i d s Steroids Extraction Steroids from Urine and/or Plasma and Analysis by LC, LC/MS or GC/MS Corticosteroids are an important class of compounds that have uses as therapeutic agents as well as some illicit uses. However, testing for them has become more challenging due to the use of drug-masking agents by athletes in an attempt to cover their steroid use. Such interferences make chemical and ELISA-based tests sometimes impractical for accurate detection. The combination of mass spectroscopy, improved chromatography separations and efficient sample preparation by SPE allows for a specific, sensitive and accurate method for detection of steroid usage. SPE LC/MS Solutions Premier Solution: Strata -X While standards can be directly analyzed on HPLC without any difficulty, interferences due to proteins, lipids, and salts require cleanup by SPE prior to injection for plasma and urine samples. SPE using Strata-X removes most matrix contaminants from the sample that would interfere with UV and MS detection, as well as allows for concentration of dilute samples. When compared to the commonly used neutral phase SPE sorbents, such as C8, Strata-X retention allows for stronger organic washes for more optimal cleanliness and longer column lifetime. Premier Solution: Gemini C8 Most steroids are very similar in structure, as well as in molecular weight. Therefore, the need for an HPLC column that can achieve baseline resolution of steroids is critical for accurate quantitation by HPLC-UV. Gemini C8 columns can provide the hydrophobic selectivity needed for most major corticosteroids down to levels around 50 ng/ml with good linearity (r > 0.995) and accuracy (98 % - 05 %). While UV detection may be sensitive enough to detect current steroid usage and monitor steroid levels for therapeutic treatments, detection of past steroid usage may require the more sensitive detection offered by a tandem mass spectrometry. The sensitivity of using MS/MS detection allows quantification of background endogenous steroids (cortisone and hydrocortisone) found in blank urine. With MS detection, such low level endogenous amounts must be taken into H account with any testing method for illicit steroid use. Linear quantification data using MS detection with Gemini columns deliver excellent linearity and reproducibility for such methods, and are powerful tools for quantitation of steroid levels in plasma and urine. Gemini C8 columns excel in volatile MS-friendly buffers making them the ideal choice for these methods. *Please see T-007, "Testosterone on Strata-X vs. Strata-X C8-E" SPE Method Sorbent: Strata-X, 0 mg/ ml Part o.: 8B-S00-TAK Condition: ml methanol followed by ml water Load: ml spiked human serum or urine (diluted : with water) Wash: ml water followed by ml 40 % methanol in water (up to %)* Elute: ml methanol ( x 500 µl) Evaporate: Under nitrogen stream (ambient) Reconstitute: Add 00 μl acetonitrile/water 40:60 Prednisolone H Cortisone H H H H H H H H H H H H H

21 p. 9 Steroids in Plasma-LC-UV Steroids in Plasma - LC-UV St e r o i d s Applications Intensity 000 Triamcinolone R = Prednisolone R = Corticosterone R = Triamcinolone acetonide R = α-hydroxyprogesterone R = Conc.(ng/mL) App ID 797 Conditions for All LC Steroid Methods: Column: Gemini 5 µm C8 Dimensions: 50 x.0 mm Part o.: 00F-445-Y0 Mobile Phase: A: 0. % Formic Acid in water B: 0. % Formic Acid in 95 % Acetonitrile / water Gradient: A/B (60:40) for 4 min to A/B (5:75) in min Flow Rate: 0.6 ml/min Detection: 54 nm or MS/MS Temperature: Ambient LC/MS/MS Chromatogram of Steroid Standard in Urine H Steroids in Urine - Standard Calibration Curves LC\MS\MS Intensity α-hydroxyprogesterone R = Deoxycorticosterone R = Ketoprogesterone R = Corticosterone R = Hydrocortisone R = Prednisone R = Conc.(ng/mL) App ID 797 Intensity. cps.9e4.0e min LC/MS/MS Chromatogram of a Blank Urine Sample Extracted ion chromatogram of different MS/MS steroids from urine blank Steroids in Urine - Standard Calibration Curves LC\MS\MS Steroids in Urine - Standard Calibration Curves LC\MS\MS Intensity Prednisolone R = Cortisone R = Cortisone acetate R = Triamcinolone acetate R = Betamethasone-7-valerate R = Triamcinolone R = Intensity. cps.8e4.6e4.4e4.e4.0e Cortisone (endogenous) Hydrocortisone min Conc.(ng/mL) Please inquire about other applications.

22 p. 0 Applications Be nzo d i a ze p i n e s Benzodiazepines Extraction of Benzodiazepines from Urine or Plasma and Analysis by LC/MS or GC/MS Benzodiazepines (benzo s) are psychoactive drugs that are prescribed for anti-anxiety. Due to their addictive nature, benzo s are highly regulated in most countries. Their possession can be a criminal offense if proper prescriptive information is not demonstrated. SPE LC/MS GC/MS Premier Solution: Strata -X and Strata -X-CW Low Cost Solution: Strata Screen-C There are a number of benzodiazepine related compounds and extraction efficiency depends on the set of compounds being analyzed. General methods can rely primarily on hydrophobic interactions using polymeric sorbents such as Strata-X. This sorbent is suitable for several different classes of compounds with recoveries between 70-0 %. Mixed-mode weak ion-exchangers such as Strata-X-CW can also be used to achieve high recoveries for certain benzo s with high pk a values. Solutions Improved Solution: Luna C8() and Synergi Fusion-RP Benzo s are well-suited to analysis by reversed phase HPLC analysis without derivatization. Eliminating this time consuming step can significantly improve sample throughput. High speed LC/MS analysis can be done using the Luna C8() or Synergi Fusion cartridges. These.5 µm materials provide high column efficiency allowing for complete analysis of eight benzo s in under minutes. Premier Solution: Zebron ZB-Drug- Standard Solution: Zebron ZB-5MSi Recovery of Benzodiazepines by SPE SPE Method: Sorbent: Strata-X-C, 60 mg/ ml Part o.: 8B-S09-UBJ Condition: ml methanol followed by ml water Load: ml spiked with analytes and IS (0.8 µg/ml); dilute : with 00 mm KH P 4 (ph 6); sample acidified with % H P4 Wash : ml 0. HCl Wash : ml methanol Dry: 0 min under vacuum Elute: ml 5 % H 4 H/methanol ( x ml) Evaporate: Dry down under slow stream of nitrogen Reconstitute: Add 00 µl mobile phase SPE Method: Sorbent: Strata-X-CW, 0 mg/ ml Part o.: 8B-S05-TAK Condition: ml methanol followed by ml D.I. water Load: ml analyte spiked human urine (dilute 500 µl urine with 5 mm sodium acetate, ph 4.5 (:) Wash : ml 5 mm sodium acetate, ph 4.5 CI Wash : ml methanol/acetonitrile/ % acetic acid (::8) Dry: min at 0 inches of Hg Elute: ml Ethylacetate. Add 50 µl of Flurazepam (0.0 μg/ml) ( x 500 µl) Evaporate: Under slow stream of nitrogen to dryness (no heat) Reconstitute: Add 00 µl 5 % acetonitrile in water App ID 604 Benzodiazepines on a Synergi Fusion-RP.00e6 9.00e5 8.00e5 7.00e5 6.00e5 5.00e5 4.00e5.00e5.00e5.00e min Column: Synergi.5 µm Fusion-RP MercuryMS Cartridge Dimensions: 0 x.0 mm Part o.: B0-CE Mobile Phase: A: 0. % Formic Acid in Water B: 0. % Formic Acid in Acetonitrile Gradient: 85:5 (A/B) to 5:95 in min, re-equilibrate to 85:5 in min Flow Rate: 0.6 ml/min Detection: MS Injection: 0 µl of 00 ng/ml Benzodiazepines in urine after SPE cleanup Sample:. 7-Aminoflunitrazepam. Chlordiazepoxide. xazepam 4. ordazepam 5. Clonazepam (IS) 6. Temazepam 7. Flunitrazepam 8. Diazepam ote: Holder required. See page for ordering information. Benzodiazepines on a Luna C8() % Recovery ordazepam Diazepam xazepam Lorazepam Strata-X-CW 98 Temazepam Bromazepam Flurazepam Diazapam Flunitrazepam Strata-X-C App ID e6.00e6 9.00e5 8.00e5 7.00e5 6.00e5 5.00e5 4.00e5.00e5.00e5 6 7 Column: Luna µm C8() MercuryMS Cartridge Dimensions: 0 x.0 mm Part o.: 00M-448-B0-CE Mobile Phase: A: 0. % Formic Acid in Water B: 0. % Formic Acid in Acetonitrile Gradient: A/B (85:5) to A/B (5:95) in min to (85:5) hold for min Flow Rate: 0.6 ml/min Detection: MS Sample:. 7-Aminoflunitrazepam. Chlordiazepoxide. xazepam 4. ordazepam 5. Clonazepam (IS) 6. Temazepam 7. Flunitrazepam 8. Diazepam.00e min ote: Holder required. See page for ordering information. CI

23 p. Diazepam Bromazepam H Be nzo d i a ze p i n e s Applications CI Flunitrazepam Br Flurazepam - + F CI F GC/MS Cut off 00 ng/ml Derivatization MTBSTFA* (with % MTBDMCS) Mass Ion(s) Quant Ion Secondary Ion Tertiary Ion D5-ordiazepam-TBDMS (IS) 4 ordiazepam-tbdms D5-xazepam-TBDMS (IS) xazepam-tbdms Serax D5-Temazepam-TBDMS (IS) Temazepam-TBDMS Restoril Lorazepam-TBDMS Ativan Clonazepam Clonopin Diazepam Valium 56 8 Alprazolam Xanax D5-α-Hydroxyalprazolam-TBDMS (IS) α-hydroxyalprazolam-tbdms App ID 7808 App ID 7894 Benzodiazepines on a Zebron ZB-Drug- 7808, 4, min 7894 Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G0-08 Injection: Split 80 C, 0. µl Carrier Gas: 55 cm/sec (constant flow) ven Program: 00 C to 0 0 C/min to 0 0 ºC for min Detection: 0 C Detection: 0 C 9 6,7 8, Benzodiazepines on a Zebron ZB-5MSi Column: Zebron ZB-5MSi Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G08-08 Injection: Split 60 C, µl Carrier Gas: ml/min (constant flow) ven Program: 00 C to 40 5 C/min for min Sample: Analytes are 4 ppm derivatized with MTBSTFA. Diazepam. ordiazepam-d5. ordiazepam 4. xazepam-d5 5. xazepam 6. Temazepam-d5 7. Temazepam 8. Clonazepam 9. Lorazepam 0. Alprazolam. α-hydroxyalprazolam-d5. α-hydroxyalprazolam Sample: Analytes are 4 ppm derivatized with MBSTFA. Diazepam 7. Temazepam. ordiazepam-d5 8. Clonazepam. ordiazepam 9. Lorazepam 4. xazepam-d5 0. Alprazolam 5. xazepam. α-hydroxyalprazolam-d5 6. Temazepam-d5. α-hydroxyalprazolam 0 4, 5 6, min Please inquire about other applications.

24 p. Applications Ba r b i t u r at e s Barbiturates Extraction of Barbiturates from Urine or Plasma and Analysis by LC, LC/MS or GC/MS Barbiturates are depressants that are typically classified into ultrashort-, short-, intermediate-, and long-acting varieties. When abused, they cause feelings similar to intoxication and induce a mellow or relaxed state. Solutions Premier Solution: Strata -X-C SPE Low Cost Solution: Strata Screen-C Barbiturates are weakly acidic in nature but can be retained through hydrophobic and hydrogen bonding interactions in SPE. Washing with acidified organic allows for removal of matrix contamination. Premier Solution: Synergi Max-RP Barbiturates can be analyzed by HPLC without derivatization using reversed phase LC, such as with the Synergi Max-RP C column. The Synergi Max-RP C column is also available in the.5 μm HST (High Speed Technology) format. These highly efficient HPLC/MS columns allow for rapid LC/MS analysis. Premier Solution: Zebron ZB-Drug- GC/MS Standard Solution: Zebron ZB-5MSi Analysis by GC/MS requires derivatization to improve quantitation. Derivatization with trimethylanilinium hydroxide (TMPAH) allowed five controlled barbiturates to be separated in less than 5 minutes. Recovery of Barbiturates by SPE 0 H Butalbital H % Recovery H Amobarbital Phenobarbital Butalbital Amobarbital Strata Screen-C Phenobarbital Butalbital Amobarbital Strata-X-C SPE Method: Sorbent: Strata-X-C, 60 mg/ ml Part o.: 8B-S09-UBJ Condition: ml methanol followed by ml water Load: ml analyte and IS spiked human urine (dilute : with water) Wash : ml 5 % methanol in water Wash : ml 0 % methanol / % acetic acid Dry: 0 in. of Hg Elute: ml 5 % methanol / % ammonium hydroxide Evaporate: At 40 ºC under slow stream of Reconstitute: Add 00 µl mobile phase SPE Method Sorbent: Strata Screen-C, 50 mg/6 ml Part o.: 8B-S06-SCH Condition: ml methanol followed by ml water Load: ml human urine spiked with analytes and IS (0.5 µg/ml); dilute : with water Wash : ml 5 % methanol in water Wash : ml 0 % methanol / % acetic acid, Dry min under vacuum Elute: ml methanol Evaporate: Dry 40 ºC under slow stream of nitrogen Reconstitute: Add 00 µl mobile phase buffer H Phenobarbital H H

25 p. GC/MS Cut off Derivatization 00 ng/ml TMPAH (optional) Mass Ion(s) Quant Ion Secondary Ion Tertiary Ion D5-Butalbital (IS) H Ba r b i t u r at e s Applications Butalbital Amobarbital Pentobarbital Secobarbital D5-Phenobarbital (IS) 5 Phenobarbital 46 7 App ID 778 Barbiturates on a Zebron ZB-5MSi 4 Column: Zebron ZB-5MSi Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G08-08 Injection: Split 60 C, µl Carrier Gas: 0.6 ml/min (constant flow) ven Program: 00 C to 0 0 C/min for 5 min Detection: 0 C Sample: Analytes are ppm derivatized with TMPAH. Butalbital-d5. Butalbital. Amobarbital 7 4. Pentobarbital 5. Secobarbital 6. Phenobarbital-d Phenobarbital 5 App ID 780 Barbiturates on a Zebron ZB-Drug- 780, 4 Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G0-08 Injection: Split 60 C, µl Carrier Gas: 0.7 ml/min (constant flow) ven Program: 45 C to 00 5 C/min Detection: 0 C H Sample: Analytes are ppm derivatized with TMPAH. Butalbital-d5. Butalbital. Amobarbital 4. Pentobarbital 5. Secobarbital 5 6. Phenobarbital-d Phenobarbital min min App ID 46 Barbiturates on a Synergi Max-RP Column: Synergi 4 µm Max-RP Dimensions: 50 x 4.6 mm Part o.: 00F-47-E0 Mobile Phase: A: 0 mm KH P 4, ph 7.0 B: Acetonitrile Gradient: A/B (80:0) hold min to A/B (50:50) in min to A/B (80:0) in min Flow Rate: ml/min Detection: 0 nm Sample:. Phenobarbital. Butabarbital. Butalbital 4. Amobarbital 4 5. Secobarbital min Please inquire about other applications.

26 p. 4 Applications 6-MAM 6-MAM Extraction of 6-Monoacetylmorphine (6-MAM) from Urine and Analysis by GC/MS 6-Monoacetylmorphine is a unique metabolite of heroin and can be used to differentiate between users of heroin and other legal and illegal opiate drugs. SPE GC/MS Solutions Premier Solution: Strata -X or X-CW Low Cost Solution: Strata Screen-C Extraction of 6-MAM is similar to other opiate related species. A mixed-mode cation-exchange sorbent such as Screen-C or Strata-X-C ionically retains 6-MAM, and wash solutions with up to 00 % methanol are used to remove weakly bound matrix interferences. However, the use of ammonium hydroxide for elution can make the acetate group in 6-MAM unstable, converting it into morphine. To avoid any potential problems, use a weak cation exchanger such as Strata-X-CW or a neutral sorbent such as Strata-X. Strata-X-CW sorbent can be neutralized by eluting with % formic acid in methanol or any other oganic solvent. Premier Solution: Zebron ZB-Drug- Standard Solution: Zebron ZB-5MSi Heroin can be metabolized into 6-Monoacetylmorphine (6-MAM) and -Monoacetylmorphine. 6-MAM can be further metabolized into morphine, so is often found in samples as well. By scanning simply for the 6-MAM ions, high sensitivity confirmation of heroin abuse can be determined. If you wish to detect other opiates as well, simply adjust the SIM ion program to include ions for those analytes. Analysis of all three analytes is possible using the same GC/MS column. GC/MS Cut off Derivatization 0 ng/ml BSTFA (with % TMCS) 6-MAM H H H H Morphine App ID 7789 Mass Ion(s) Quant Ion Secondary Ion, Tertiary Ion 6-Monoacetylmorphine (6-MAM) D5-6-Monoacetylmorphine (6-MAM d5) (IS) MAM on a Zebron ZB-5MSi Column: Zebron ZB-5MSi Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G08-08 Injection: Split 80 C, µl Carrier Gas: 0.7 ml/min (constant flow) ven Program: 70 C to 00 0 C/min Detection: 0 C Sample: Analytes are ppm derivatized with BSTFA. 6-MAM-d. 6-MAM Recovery of 6-MAM by SPE min % Recovery Strata-X-CW Strata-X App ID MAM on Zebron ZB-Drug-, Column: Zebron ZB-Drug- Dimensions: 0 meter x 0.8 mm x 0.8 µm Part o.: 7CD-G0-08 Injection: Split 60 C, µl Carrier Gas: 0.7 ml/min (constant flow) ven Program: 45 C to 00 5 C/min Detection: 00 C Sample: Analytes are ppm derivatized with BSTFA. 6-MAM-d. 6-MAM SPE Method: Sorbent: Strata-X, 60 mg/ ml Part o.: 8B-S00-UBJ Condition: ml methanol followed by ml water Load: ml spiked human urine (diluted : with water) Wash: ml water followed by ml 0 % methanol/water Elute: ml methanol Dry: min at 0 in. Hg Evaporate: Blow down to dryness under nitrogen at 40 C Reconstitute: Add 0.50 µl ethylacetate and 50 µl BSTFA ( % TMCS). React at 70 C for 0 min min

27 p. 5 Blood Alcohols Fast and Accurate Blood Alcohol Analysis by GC/FID Blood alcohol analysis is typically used by law enforcement to determine if a driver was unlawfully operating a vehicle. The results will be used in a court of law, therefore it is important to minimize systematic and operator errors. Blood Alcohols Applications GC/MS Solutions Premier Solution: Zebron ZB-BAC and The new Zebron ZB-BAC and column pair is optimized for fast ethanol separation. These columns resolve both t-butanol and n-propanol from other volatile solvents, enabling the use of either compound as the internal standard. This is advantageous because n-propanol, which may be present in blood samples as a result of bacterial growth, is not a reliable standard in some cases. Zebron ZB-BAC and are ideal for law enforcement applications where breathalyzer tests are not feasible as well as with post mortem samples. App ID 7897 Blood Alcohols on a Zebron ZB-BAC Column: Zebron ZB-BAC Dimensions: 0 meter x 0. mm x.80 µm Part o.: 7HM-G0- Injection: Split 40 C, 0.5 ml Carrier Gas: 80 cm/sec (constant flow) ven Program: 40 C (Isothermal) Detection: 0 C Sample:. Methanol. Acetaldehyde. Ethanol 4. Isopropanol 5. Acetone 6. t-butanol (IS) 7. -Butanol (IS) 7 App ID 7898 Confirmation of Blood Alcohols on a Zebron ZB-BAC 5 4 Column: Zebron ZB-BAC Dimensions: 0 meter x 0. mm x.0 µm Part o.: 7HM-G0-5 Injection: Split 40 C, 0.5 ml Carrier Gas: 80 cm/sec (constant flow) ven Program: 40 C (Isothermal) Detection: 0 C Sample:. Methanol. Acetaldehyde. Ethanol 4. Isopropanol 5. Acetone 6 6. t-butanol (IS) 7. -Butanol (IS) 7 0 min min GC Tip Wh e n w o r k i n g w i t h p o s t m o r t e m s a m p l e s, it is o f t e n s a f e r to u s e t-bu ta n o l a s yo u r i n t e r n a l s ta n d a r d. -Pr o pa n o l, a c o m m o n i n t e r n a l s ta n d a r d, m i g h t b e p r e s e n t a s a r e s u lt o f b a c t e r i a l g r o w t h a n d c a n g i v e yo u misleading r e s u lt s. Si n c e t h e Ze b r o n ZB-BAC a n d GC c o l u m n s co m p l e t e ly r e s o lv e t-bu ta n o l, yo u c a n u s e it a s a n i n t e r n a l s ta n d a r d a n d at ta i n m o r e accurate results. Please inquire about other applications

28 Complete Support Technical specialists available to help with your troubleshooting and method optimization Full range of method development guides and reference tools Complete technical solutions for sample preparation and HPLC/GC analysis Global Assistance Phenomenex technical specialists are available to provide technical and product support worldwide. Method Development FREE application and troubleshooting services are available to help you create optimized SPE methods for your analysis. Reference Tools Comprehensive guides and technical notes are available to meet all your SPE, GC or LC needs. Please ask your Phenomenex technical consultant for complimentary methods on Extraction of Amphetamines from Urine using Strata Screen-C (ER-000) Simplified Method Development for the Extraction of Acidic, Basic, and eutral Drugs with a Single SPE Sorbent Strata -X (T-0004) Sample Preparation of Tricyclic Antidepressants for Drug Screens: The Advantages of Strata-X over Mixed-Mode Sorbents (T-0006) LC/MS/MS Analysis of Basic Drugs with Positive Ion Electrospray in High ph Mobile Phase (T-0) Please inquire about other applications from our Application and Technote Library.

29 rdering Information

30 p. 8 rdering information Sample Prep./SPE Strata and Strata -X SPE Sorbents Strata -X Format Sorbent Mass Part. o. Unit Price Tube 0 mg 8B-S00-TAK ml (00/box)* 60 0 mg 8B-S00-TBJ ml (50/box) mg 8B-S00-UBJ ml (50/box)** mg 8B-S00-FBJ ml (50/box) mg 8B-S00-ECH 6 ml (0/box) 4 00 mg 8B-S00-FCH 6 ml (0/box) mg 8B-S00-HCH 6 ml (0/box) Well Plate 0 mg 8E-S00-AGB Plates/Box mg 8E-S00-TGB Plates/Box mg 8E-S00-UGB Plates/Box 600 *ASPEC capped tubes available in 500/bag (8B-S00-TAM-A) **Tab-less tubes available (8L-S00-UBJ) Strata -X-C Format Tube 96-Well Plate Strata -XL Format Tube 96-Well Plate Strata -XL-C Format Tube 96-Well Plate Strata Screen-C Sorbent Mass Part. o. Unit Price 0 mg 8B-S09-TAK ml (00/box) 60 0 mg 8B-S09-TBJ ml (50/box) 4 60 mg 8B-S09-UBJ ml (50/box) mg 8B-S09-FBJ ml (50/box) mg 8B-S09-ECH 6 ml (0/box) 4 00 mg 8B-S09-FCH 6 ml (0/box) mg 8B-S09-HCH 6 ml (0/box) 60 0 mg 8E-S09-AGB Plates/Box mg 8E-S09-TGB Plates/Box mg 8E-S09-UGB Plates/Box 600 Sorbent Mass Part. o. Unit Price 60 mg 8B-S04-UBJ ml (50/box) mg 8B-S04-FBJ ml (50/box) mg 8B-S04-EBJ ml (50/box) mg 8B-S04-FCH 6 ml (0/box) mg 8B-S04-HCH 6 ml (0/box) 05 0 mg 8E-S04-TGB Plates/Box 600 Sorbent Mass Part. o. Unit Price 0 mg 8B-S044-TAK ml (00/box) mg 8B-S044-UBJ ml (50/box) mg 8B-S044-FBJ ml (50/box) mg 8B-S044-ECH 6 ml (0/box) mg 8B-S044-FCH 6 ml (0/box) mg 8B-S044-HCH 6 ml (0/box) 05 0 mg 8E-S044-TGB Plates/Box 600 Format Sorbent Mass Part. o. Unit Price Tube 00 mg 8B-S06-EAK ml (00/box) mg 8B-S06-EBJ ml (50/box) 9 50 mg 8B-S06-SBJ ml (50/box) mg 8B-S06-FBJ ml (50/box) mg 8B-S06-RBJ ml (50/box) 9 50 mg 8B-S06-SCH 6 ml (0/box) 84 *00/box available (8B-S06-5BL) Strata Screen-A Format Sorbent Mass Part. o. Unit Price Tube 00 mg 8B-S09-FBJ ml (50/box) mg 8B-S09-HCH 6 ml (0/box) 8 Strata Screen-C GF Format Sorbent Mass Part. o. Unit Price Tube 00 mg 8B-S06-FBJ ml (50/box) mg 8B-S06-FCH 6 ml (0/box) mg 8B-S06-HBJ ml (50/box) mg 8B-S06-HCH 6 ml (0/box) mg 8B-S06-JCH 6 ml (0/box) 4 Strata -X-CW Format Sorbent Mass Part umber Unit Price Tube 0 mg 8B-S05-TAK ml (00/box) mg 8B-S05-UBJ ml (50/box) mg 8B-S05-FBJ ml (50/box) mg 8B-S05-FCH 6 ml (0/box) 45 SPE Vacuum Manifolds Part o. Description Unit Price 4 Position Vacuum Manifold AH0-604 SPE 4-Position Vacuum Manifold Set, complete assembly ea.40 4 Position Vacuum Manifold Replacement Parts AH0-606 SPE Glass Chamber ea 0 AH0-608 SPE Cover, Gasket and 4 Stopcocks ea 455 AH0-600 SPE Gaskets /pk 4 AH0-608 SPE Collection Rack, including shelves, legs, ea 90 clips and posts AH SPE Luer Stopcocks 4/pk 9 Position Vacuum Manifold AH0-60 SPE -Position Vacuum Manifold Set, complete assembly ea 780 Position Vacuum Manifold Replacement Parts AH0-605 SPE -Position Glass Chamber ea 55 AH0-607 SPE Cover, Gasket and Stopcocks ea 55 AH0-609 SPE Gaskets /pk 4 AH0-607 SPE Collection Rack, including shelves, legs, ea 50 clips and posts AH0-605 SPE -Position Vacuum Waste Container, 0/pk 86 polypropylene AH SPE Luer Stopcocks 4/pk 9 Strata n-line Extraction Cartridges Part o. Description Unit/Box Price 00M-S0-B0-CB Strata-X on-line extraction cartridge, ea 55 0 x.0 mm 00M-S06-B0-CB Strata-X-CW on-line extraction cartridge, ea 55 0 x.0 mm 00M-S09-B0-CB Strata C8 on-line extraction cartridge, ea 0 0 x.0 mm 00M-S0-B0-CB Strata C8 on-line extraction cartridge, ea 0 0 x.0 mm CH Cartridge holder, 0 mm ea 96 If Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE!

31 p. 9 Zebron -High Performance GC Columns ZB-Drug- ID(mm) df(µm) Temp. Limits C Part o. Price 0-Meter to 0/40 C 7CD-G Meter to 0/40 C 7EG-G0-5 0-Meter to 0/40 C 7HG-G0-55 ZB-BAC ID(mm) df(µm) Temp. Limits C Part o. Price 0-Meter to 60/80 C 7HM-G to 60/80 C 7HK-G ZB-BAC ID(mm) df(µm) Temp. Limits C Part o. Price 0-Meter to 60/80 C 7HM-G to 60/80 C 7HK-G0-55 ZB-5MSi ID(mm) df(µm) Temp. Limits C Part o. Price 0-Meter to 60/70 7CD-G Meter to 60/70 7EG-G Meter to 60/70 7HG-G to 60/70 7HG-G to 60/70 7HM-G to 60/70 7HM-G Meter to 60/70 7KG-G ZB-5ms ID(mm) df(µm) Temp. Limits C Part o. Price 0-Meter to 5/50 7CB-G to 5/50 7CD-G Meter to 5/50 7EG-G Meter to 5/50 7FD-G to 5/50 7FD-G to 5/50 7FD-G Meter to 5/50 7HG-G to 5/50 7HG-G to 5/50 7HG-G to 5/50 7HM-G to 5/50 7HM-G to 5/50 7HM-G Meter to 5/50 7KG-G to 5/50 7KM-G GC Co l u m n s ZB-ms ID(mm) df(µm) Temp. Limits C Part o. Price 0-Meter to 60/70 7CB-G to 60/70 7CD-G Meter to 60/70 7DE-G Meter to 60/70 7EG-G to 60/70 7EM-G Meter to 60/70 7FD-G Meter to 60/70 7GE-G Meter to 60/70 7HG-G to 60/70 7HG-G to 60/70 7HG-G to 60/70 7HG-G to 60/70 7HM-G to 60/70 7HM-G Meter to 60/70 7KG-G to 60/70 7KG-G to 60/70 7KM-G0-765 ZB-XLB ID(mm) df(µm) Temp. Limits C Part o. Price 0-Meter to 40/60 7CD-G Meter to 40/60 7EG-G Meter to 40/60 7FD-G Meter to 40/60 7HG-G to 40/60 7HG-G to 40/60 7HM-G to 40/60 7HM-G to 0/40 7HK-G Meter to 40/60 7KG-G rdering information If Zebron columns do not provide you with equivalent separations as compared to any other GC column of the same phase, and comparable dimensions, send in your comparative data within 45 days and keep the Zebron column for FREE.

32 p. 0 rdering information Gen er al GC Access o r i es General GC Accessories Septa Silicone Rubber Inlet Septa Advanced silicone formulations reduce coring and increase septum lifetime Enhanced durability and re-sealing capabilities PhenoGrey -50 This general purpose silicone rubber septum is designed for use up to 50 C for extended periods of time. With a durometer reading of 40-45, septum puncture is easy (fewer bent needles) for up to 00 injections. PhenoBlue -00 This low-bleed septum is designed from a recent technology advance in the silicone field. The high durometer rating of guarantees over 00 injections at 00 C, but is actually heat-stable to 50 C to give you even greater confidence when the oven heats up! PhenoRed -400 Temperature-rated to 400 C, even continuous injection port temperatures of 50 C rarely change this septum's physical properties. Typical injection life is 50 punctures. Available with or without needle GuideRight Hole. PhenoGreen -400 Long life, high-temperature green septum, rated to 400 C. GuideRight through-hole to guide the needle during injection. Ferrules Graphite Ferrules High-purity graphite significantly reduces ferrule bleed Special construction minimizes "flaking" Graphite Ferrules, Rated to 450 C Part o. Description Unit Price 0.4 mm Ferrule ID AG Graphite Ferrule /6 in. to 0.4 mm 0/pk 4 AG Graphite Ferrule /6 in. to 0.4 mm 50/pk mm Ferrule ID AG0-470 Graphite Ferrule /6 in. to 0.5 mm 0/pk 4 AG0-470 Graphite Ferrule /6 in. to 0.5 mm 50/pk mm Ferrule ID AG Graphite Ferrule /6 in. to 0.8 mm 0/pk 4 AG Graphite Ferrule /6 in. to 0.8 mm 50/pk 45 Septa Sizes by GC Instrument Manufacturer Instrument Model Septa Diameter Agilent (HP) 5850, 5880, 5890, 6850, /6 in. ( mm) 5700 series, 5880A /8 in. (9.5 mm) Silicone Rubber Septa Part o. Description Unit Price PhenoGrey /8 in. (9.5 mm) Diameter AG PhenoGrey-50 Injector Septa 50/pk 4 AG PhenoGrey-50 Injector Septa 00/pk 7 PhenoBlue AG PhenoBlue-00 Injector Septa 50/pk 7 AG PhenoBlue-00 Injector Septa 00/pk 4 PhenoRed AG PhenoRed-400 Injector Septa 50/pk 80 AG0-469 PhenoRed-400 Injector Septa 00/pk 45 PhenoGrey 7/6 in. ( mm) Diameter AG0-469 PhenoGrey-50 Injector Septa 50/pk 4 AG0-469 PhenoGrey-50 Injector Septa 00/pk 7 PhenoBlue AG PhenoBlue-00 Injector Septa 50/pk 7 AG PhenoBlue-00 Injector Septa 00/pk 4 PhenoRed AG PhenoRed-400 Injector Septa 50/pk 80 AG PhenoRed-400 Injector Septa 00/pk 45 Short Ferrules Vespel/Graphite (85/5 %) Short Ferrules (not for GC/MS), Rated to 50 C Part o. Description Similar to Mfr. o.* Unit Price AG0-78 Vespel/Graphite (85/5 %) 58-0/pk 5 Short Ferrules, /6 in. to 0.4 mm (for 0.0 to 0.5 mm ID columns) AG0-79 Vespel/Graphite (85/5 %) /pk 5 Short Ferrules, /6 in. to 0.5 mm (for 0. ID columns) AG0-70 Vespel/Graphite (85/5 %) Short Ferrules, /6 in. to 0.8 mm (for 0.45 to 0.5 mm ID columns) /pk 5 Graphite (00 %) Short Ferrules (not for GC/MS), Rated to 450 C Part o. Description Similar to Mfr. o.* Unit Price AG0-75 Graphite (00 %) Short Ferrules, /6 in. to 0.5 mm /pk 4 Vespel/Graphite (85/5 %) Short Ferrules GC/MS Compatible, Rated to 50 C Part o. Description Similar to Mfr. o.* Unit Price AG0-7 Vespel/Graphite (85/5 %) GC/MS 58-0/pk 55 Short Ferrules, /6 in. to 0.4 mm (for 0.0 to 0.5 mm ID columns) AG0-7 Vespel/Graphite (85/5 %) GC/MS /pk 55 Short Ferrules, /6 in. to 0.5 mm (for 0. ID columns) AG0-7 Vespel/Graphite (85/5 %) GC/MS Short Ferrules, /6 in. to 0.8 mm (for 0.45 to 0.5 mm ID columns) /pk 55 Vespel / Graphite Ferrules Vespel/Graphite Ferrules (60/40 %), Rated to 400 C Part o. Description Unit Price 0.4 mm Ferrule ID AG Vespel/Graphite Ferrule /6 in. to 0.4 mm 0/pk 55 AG Vespel/Graphite Ferrule /6 in. to 0.4 mm 50/pk mm Ferrule ID AG0-470 Vespel/Graphite Ferrule /6 in. to 0.5 mm 0/pk 55 AG0-47 Vespel/Graphite Ferrule /6 in. to 0.5 mm 50/pk mm Ferrule ID AG0-47 Vespel/Graphite Ferrule /6 in. to 0.8 mm 0/pk 55 AG0-474 Vespel/Graphite Ferrule /6 in. to 0.8 mm 50/pk 70 All Vespel containing ferrules should be pre-shrunk in an oven at 50 C for at least 4 hours prior to use. *Similar to but not always an exact equivalent to the original manufacturer's product.

33 p. General GC Accessories Cool-Lock for Agilent HP GC System Part o. Description Unit Price AG0-89 Cool-Lock GC Capillary ut For Use With Short- ea 6 Style Ferrules AG0-80 Cool-Lock GC Capillary ut For Use With Long- ea 6 Style Ferrules AG0-849 Cool-Lock ut Installation Gauge ea 70 Cool-Lock for Shimadzu GC System Part o. Description Unit Price AG0-849 Cool-Lock GC Capillary ut For Use With Short- ea 89 Style Ferrules AG0-840 Cool-Lock ut Installation Gauge ea 89 Gen er al GC Access o r i es Gold Inlet Base Seals Part o. Description Similar to Mfr. o.* Unit Price Standard, single groove for splitless applications, 0.8 mm dia. inlet hole AG0-758 Gold Inlet Base Seal, splitless (single /pk 88 groove), 0.8 mm AG0-759 Gold Inlet Base Seal, splitless (single groove), 0.8 mm /pk 80 High Split Flow, cross groove for split applications, 0.8 mm dia. inlet hole AG0-750 Gold Inlet Base Seal, split (double /pk 0 groove/cross), 0.8 mm AG0-75 Gold Inlet Base Seal, split (double groove/cross), 0.8 mm /pk 90 Replacement Inlet Seal Washers AG0-75 Inlet Seal Washers, for Agilent GC injection port /pk 8 rdering information Sealing -Rings Sealing Rings for Agilent Inlet Liners Part o. Description Similar to Mfr. o.* Unit Price AG0-76 AG0-77 Sealing ring, Viton, rated to < 00 C Sealing ring, graphite, rated to > 50 C (450 C max.) /pk /pk 7 Column Installs This End Injection Port Inlet Liners Liners Description GC Model o. Dimensions ID x L x D (mm) Splitless Straight Liner 5880/5890/ x 78.5 x 6.45 Split / Splitless with Single Taper 5880/5890/ x 78.5 x 6.45 Splitless, Double Taper/Gooseneck Liner 5880/5890/ x 78.5 x 6.45 Material* (deactivated) Agilent Technologies (HP) Quartz Wool (Y / ) otes Similar to Mfr. o.** Part o. Unit Price B (n) ea 5/pk 5/pk B (y) ea 5/pk 5/pk B (y) ea 5/pk 5/pk AG0-465 AG0-465 AG0-756 AG AG0-87 5/pk 5/pk 5/pk 5/pk /pk 60 Split / Splitless with wool 5880/5890/ x 78.5 x 6. B (y) Y ea AG /pk 9 Split / Splitless, Single Taper with wool 5880/5980/ x 78.5 x 6. B (y) Y ea 5/pk 5/pk * B= Borosilicate; Deactivated = Yes (y) or o (n). ** Similar to but not always an exact equivalent to the original manufacturer s product AG /pk 4 Contact Phenomenex or your local Phenomenex distributor for additional GC accessories. ther manufacturer GC accessories are available.

34 p. rdering information HPLC Co l u m n s HPLC Luna High Speed Technology (HST) Columns.5 μm High Speed Technology (HST) Columns (mm) Phase 0 x.0 50 x.0 00 x.0 50 x.0 00 x Luna.5 µm C8()-HST 00A-4446-B0 00B-4446-B0 00D-4446-B0 00B-4446-Y0 00D-4446-Y0 μm Microbore and Minibore Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x.0 50 x.0 50 x.0 0 x.0 50 x.0 00 x.0 50 x.0 4 x /0pk C8() 00A-448-A0 00B-448-A0 00F-448-A0 00A-448-B0 00B-448-B0 00D-448-B0 00F-448-B0 AJ0-489 C8() 00A-45-A0 00B-45-A0 00F-45-A0 00A-45-B0 00B-45-B0 00D-45-B0 00F-45-B0 AJ0-486 HILIC 00B-4449-B0 00D-4449-B0 00F-4449-B0 AJ0-88 PFP() 00A-4447-B0 00B-4447-B0 00D-4447-B0 00F-4447-B0 AJ0-86 for ID:.0-.0 mm μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.0 50 x.0 0 x x x x x.0 4 x /0 pk 5 /0 pk C8() 00B-448-Y0 00F-448-Y0 00A-448-E0 00B-448-E0 00D-448-E0 00F-448-E0 AJ0-489 AJ0-490 C8() 00B-45-Y0 00F-45-Y0 00A-45-E0 00B-45-E0 00D-45-E0 00F-45-E0 AJ0-486 AJ0-487 HILIC 00B-4449-Y0 00F-4449-Y0 00D-4449-E0 00F-4449-E0 AJ0-88 AJ0-89 PFP() 00B-4447-Y0 00F-4447-Y0 00B-4447-E0 00D-4447-E0 00F-4447-E0 AJ0-86 AJ0-87 for ID:.0-.0 mm.-8.0 mm 5 μm Microbore and Minibore Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x.0 50 x.0 50 x.0 4 x /0 pk C8 () 00A-449-B0 00B-449-B0 00F-449-B0 AJ0-489 C8 () 00A-45-B0 00B-45-B0 00F-45-B0 AJ0-486 PFP() 00A-4448-B0 00B-4448-B0 00F-4448-B0 AJ0-86 for ID:.0-.0 mm 5 μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.0 50 x.0 0 x x x x.0 4 x /0 pk 5 /0 pk C8() 00B-449-Y0 00F-449-Y0 00A-449-E0 00B-449-E0 00C-449-E0 AJ0-489 AJ0-490 C8() 00B-45-Y0 00F-45-Y0 00A-45-E0 00B-45-E0 00C-45-E0 AJ0-486 AJ0-487 PFP() 00B-4448-Y0 00F-4448-Y0 00A-4448-E0 00B-4448-E0 AJ0-86 AJ0-87 for ID:.0-.0 mm.-8.0 mm 5 μm Analytical Columns (mm) continued SecurityGuard Cartridges (mm) Phases 00 x x x x /0 pk C8() 00D-449-E0 00F-449-E0 00G-449-E0 AJ0-490 C8() 00D-45-E0 00F-45-E0 00G-45-E0 AJ0-487 HILIC 00D-4450-E0 00F-4450-E0 00G-4450-E0 AJ0-89 PFP() 00D-4448-E0 00F-4448-E0 00G-4448-E0 AJ0-87 for ID:.-8.0 mm Synergi HPLC Columns 4 µm Microbore and Minibore Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.0 50 x.0 50 x.0 0 x.0 50 x.0 00 x.0 50 x.0 50 x.0 4 x /0pk Max-RP 00B-47-A0 00F-47-A0 00A-47-B0 00B-47-B0 00D-47-B0 00F-47-B0 00G-47-B0 AJ0-607 Hydro-RP 00B-475-A0 00F-475-A0 00G-475-A0 00A-475-B0 00B-475-B0 00D-475-B0 00F-475-B0 00G-475-B0 AJ0-750 Fusion-RP 00B-444-A0 00F-444-A0 00A-444-B0 00B-444-B0 00D-444-B0 00F-444-B0 00G-444-B0 AJ for ID:.0-.0 mm 4 µm arrow Bore Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.0 75 x.0 00 x.0 50 x.0 50 x.0 4 x /0pk Max-RP 00B-47-Y0 00C-47-Y0 00D-47-Y0 00F-47-Y0 00G-47-Y0 AJ0-607 Hydro-RP 00B-475-Y0 00C-475-Y0 00D-475-Y0 00F-475-Y0 00G-475-Y0 AJ0-750 Fusion-RP 00B-444-Y0 00C-444-Y0 00D-444-Y0 00F-444-Y0 00G-444-Y0 AJ for ID:.0-.0 mm 4 µm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x x x x x x /0pk Max-RP 00A-47-E0 00B-47-E0 00D-47-E0 00F-47-E0 00G-47-E0 AJ Hydro-RP 00A-475-E0 00B-475-E0 00D-475-E0 00F-475-E0 00G-475-E0 AJ0-75 Fusion-RP 00A-444-E0 00B-444-E0 00D-444-E0 00F-444-E0 00G-444-E0 AJ for ID:.-8.0 mm SecurityGuard Analytical Cartridges require universal holder Part o.: KJ-48