Brachypodium distachyon,, a new model plant. Richard Sibout
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1 Brachypodium distachyon,, a new model plant Richard Sibout
2 ALICE: Brachypodium seed stocks Leaf DNA M2 Phenotyping (visual and chemical) (Versailles) 6 seeds (plants) Mutagenesis M1 M2 seeds production Versailles Mutagenized seeds Leaf DNA M2 Tilling (URGV-Evry) 10 M2 seeds (plants) M3 seeds stocks (Versailles) M2 seeds stocks (Versailles) M2 Saccharification Leonardo Gomez (York-UK) M3 seeds stock Backup (York-UK)
3 Emergence stage Young plants not seedled Dead at early stage delay Devlopment strong delay Plantlet Visual phenotyping: From sawing to harvest albinos Vegetative stage Mature plants Whole Stem plant Leaf Shape Color Size Color Shape & arrangement Appearance Flowering stage Mature plants Spike culm Flowering time Color Organisation Height & appearance Architecture Tillering / Branching
4 Visual phenotyping: From sawing to harvest nb phenotypes no germination 234 dead dead dead at early stage 7 early devlopment 0 late devlopment 10 plant devlopment plant devlopment strong late devlopment 6 Erected plant 129 Fairly Erected 120 Poorly Erected 20 architecture compact 0 compact at the top 0 hyper compact 0 giant with long internodes 0 giant with normal internodes 0 long internodes 0 Small with less internodes 5 Plant architecture height and appearance small with Short internodes 16 miniature 13 dwarf(1/4-2/3 of N) 7 extrem dwarf 7 no tiller 1 tillers low tillers number 10 high tillers number 21 no branching 0 branching type low branching 0 strong branching 1 very narrow 8 narrow 23 size small 27 large 0 cholorotic 1 albinos 1 glossy 0 beige midrib 1 brown midrib 0 red midrib 0 beige margins 1 brown margins 0 red margins 0 color dark green 20 pale green 16 light green 25 light green stripes 1 vein/lam - veining 1 variegated 0 yellow 1 dégradé jaune à la base vert en haut de la 1 Virescent / Albescent 1 white upper leaf 8 leaf etiolated upper leaf 1 adherent 3 brittled 0 crinkled 9 drooped 0 mottled 9 necrosed 17 appearance spotted 0 stunted 0 tearing 0 thick 0 twisted 1 waxy 0 wilted 0 rhomboid-pointed 0 round 0 indented edge 1 distorted 0 shape and arrangements downcurling 0 margin raised 0 upcurling 1 coneshaped at leaf base 0 leaflets superimposed 0 thin 36 floppy 1 shape thick 1 stem fasciated 1 curved internode 10 color brown 2 Head Head Short internode between spikelets 2 early flowering 28 flowering time late flowering 4 Spikelet trapped in stem and upper leaf 1 no flower 0 low flower / spike 5 sterile flowers 4 flowers reproductive organs Abnnormal spike 20 white spike 20 partial white spike 21 cauliflower type inflorescence 0 abnormal all 0 beige 0 flower morphology pale 0 small 27 seed color large 0 crinkled 9 seed size crackled seed coat 0 wrinkled 0 shape extremely wrinkled 0 61 different phenotypes have been scored thin 36 floppy 1 stem shape thick 1 fasciated 1 curved internode 10 color brown 2 Head Head Short internode between spikelets 2 flowering time early flowering 28 late flowering 4 flowers reproductive organs flower morphology Spikelet trapped in stem 1 and upper leaf no flower 0 low flower / spike 5 sterile flowers 4 Abnnormal spike 20 white spike 20 partial white spike 21 cauliflower type inflorescence 0 abnormal all 0 beige 0 pale 0
5 Examples of mutants affected in leaf and stem development Erected Virescent Curved stems wt Leaf color Unbranched Dwarf
6 Examples of mutants affected in spike, leaf and stem formation Scorpion tail spikelets Short or long spikelets wt Aborted spikes Abnormal stems Brown-midrib leaf
7 Natural accessions are genetic resources for cell wall variability Bd21.3 Bd21 Bd3.1 Bd18
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