Table 9.1.: Potometer Readings. Calculation of leaf Surface Area

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1 Table 9.1.: Potometer Readings At the end of your experiment, cut all the leaves off the plant and mass them. Remember to blot off all excess water before Mass of leaves.~ grams. Calculation of leaf Surface Area The total surface area of all the leaves can be calculated the Leaf Mass Method. either the Leaf Trace Method or leaf Trace Method After arranging all the cut-off leaves on the grid below, trace the pattern directly onto Grid 9.1. Count all of the that are within the and estimate the number of that lie partially within the has been constructed so that a square of 4 blocks equals 1 cm 2 The total surface area can then be calculated by dividing the total number of blocks covered by 4. Record this value here: = Leaf Surface Area Grid 9.1.

2 Leaf Mass Method Cut a 1 cm 2 section out of 1 leaf. Mass the 1 cm 2 section. Multiply the section's mass by 10,000 to calculate the mass per square meter of the leaf. (g/m2)... Divide the total mass of the leaves 11) the mass per square meter This value is the leaf surface area. Total Mass of Leaves g/m2 Record this value here:.~ ~... Leaf Surface Area Leaf Surface Area 12. Calculate the water loss per square meter of leaf sulface by the water loss at each reading 9.1) by the leaf surface area you calculated. Record your results in Table 9.2. Table 9.2: ndividual Water Loss in ml/m 2 Time nterval (minutes) Water Loss (ml) :LS Water loss per m2 i 13. Record the averages of the class data for each treatment in Table 9.3. Table 9.3: Class Average Cumulative Water Loss in ml/m2 Time (minutes) Treatment Room 0 Light Fan 0 0 Mist 0

3 14. For each treatment, graph the average of the class data for each time interval. You may need to convert data to scientific notation. All numbers must be reported to the same power of 10 for purposes. For this graph you will need to determine the following: 3. The independent vatiable:. Use this to label the horizontal axis. b. The variable: Use to label the vertical (y) axis. Graph 9.1 Title:

4 Analysis of Results 1. Calculate the rate (average amount of water loss per minute per square for each of the treatments, Room: Fan: Mist: 2. Explain why each of the conditions causes an increase or decrease in transpiration compared with the control Condition 'Effect Explanation of Effect Room Fan Light Mist 3. Explain the role of water potential in the movement of water from soil through the plant and into the air. 4. What is the advantage of closed stomata to a plant when water is in short supply? What are the disadvantages? 5. Describe several both structural and reduce water loss from their leaves, nclude

5 AP LAB 9B Station 1 1. s this a monocot or a woody eudicot 2. The and green section is the and the tan and brown section the ~ both of which make up the plants system. Station 2 3. Look at the plant in the graduated cylinder. Which plant is showing the deficiency, the old or new growth 7 Station 3 4. Look at sweet pea plants. The ends of the stems have are 5. s this a monocot or a into which Station 4 6. dentify the structures: The 3 pink structures The 4 green structures Station 5 7. s this a model of a monocot or a eudicot? 8. dentify the following structures: 13 and 14 1 Station 6 9. Draw the moss antheridial head. s this the male or female structure? gametophyte or sporophyte?

6 Station Draw the moss head. s this the or female gametophyte or sporophyte? Station Draw the Fern Antheridia and Archegonia. label your with male and Station Draw the young fern sporophyte. s this the dominant structure in the fern Station Draw the eudicot root. label the vascular bundles and dermis

7 Station Draw the monocot root. label the vascular bundles, dermis, and endodermis. Station Draw the eudicot stem. Label the vascular bundles, dermis and ground tissue. Station Draw the monocot stem. Label the vascular bundles, dermis and ground tissue. Station Draw the lily anther. s this the male or female structure? What is produced here? Station Draw the dicot leaf. Label the cuticle and the vascular bundles.

8 ...T:;o'''nn Draw the germinated pine pollen. label the pollen and pollen grain. Station Draw the mature pine pollen. the Station Draw all three of the combination structures and identify what they are. Station Draw the pine older stem. Label the phloem, xylem and woody tissue.

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