Chemistry 2. Molecular Photophysics
|
|
- Norman Jones
- 5 years ago
- Views:
Transcription
1 Chemistry 2 Lecture 12 Molecular Photophysics
2 Assumed knowledge Electronic states are labelled using their spin multiplicity with singlets having all electron spins paired and triplets having two unpaired electrons. After absorption, energy is lost by radiative transitions and non radiative transitions. Fluorescence spectra are red shifted compared to absorption spectra but commonly have mirror symmetry Learning outcomes Be able to explain Kasha s law by describing internal conversion Be able to define fluorescence quantum yield Be able to describe intersystem crossing and how it leads to phosphoresence Be able to explain why the phosphorescence occurs at lower energy ( red shifted ) and is slower than fluorescence
3 last lecture S 1 T 1 CH 3 CH 3 CH 3 CH 3 CH 3 O trans-retinal (light absorber in eye) S 0
4 Absorption spectrum of a dye S S Absorba ance S 3 S S S S 0 Wavelength (nm)
5 Absorption to several electronic states A = εclε Benzene
6 Showing singlet and triplet absorption S 3 Triplet st S 2 T 2 S 1 T 1 S 0 Absorption to triplet states from single states is formally forbidden, thus very weak.
7 Fluorescence spectrometer Key features: Two monochromators are needed one to select a single wavelength to excite the molecule the other to resolve the emitted wavelengths Excitation and detection occur at 90 to each other this minimises the amount of incident light gets into the detector.
8 Fluorescence spectrometer Two types of spectra: Fluorescence spectrum select excitation λ with mono #1 scan mono #2 to measure fluorescence spectrum Excitation spectrum later
9 Real data Absorption Fluorescence Wavelength (nm) Fluorescence is always to longer wavelength Stokes shift = (absorbance max) (fluorescence max) = 50 nm here Mirror symmetry
10 Fluorescence spectrum f(λ exc ) NRD Note: the non-radiative decay (NRD) only occurs in the condensed phase, where the fluorophore can transfer vibration energy to the solvent.
11 Stokes shift Absorption The shift between λ max (abs.) and λ max (fluor) is called the STOKES SHIFT max( ) max( ) A bigger Stokes shift will produce more dissipation of heat
12 Franck Condon Principle (in reverse) gy Energ R
13 Franck Condon Principle (in reverse) gy Energ R Note: If vibrational frequencies in the ground and exited state are similar, then the spectra look the same, but reversed -> the so-called mirror symmetry
14 Absorption spectrum of another dye 200 λ = 400 nm Excite dye at different wavelengths Abso orbance λ = 440 nm λ = 550 nm Wavelength (nm) What will emission (fluorescence) spectra look like?
15 The emission is the same! λ ex = 400 nm λ ex = 440 nm λ ex = 550 nm * * * Wavelength (nm)
16 Kasha s Law: Emission always occurs from the lowest excited electronic state t S 2 Internal Conversion (IC) NRD S 1 Emission S 0 Internal conversion (IC) is the spontaneous relaxation of an electron to a lower energy Internal conversion (IC) is the spontaneous relaxation of an electron to a lower energy state, accompanied by a simultaneous increase of vibrational energy of the molecule. IC is a non radiative process.
17 Born Oppenheimer Breakdown Terms in the Hamiltonian operator which are ignored when making the BO approximation promote transitions between states of the same energy in the molecule. Small energy gap = Good Franck-Condon factor Large energy gap = Bad Franck-Condon factor A molecule can make a non-radiative transition to an isoenergetic state. If this state can then lose vibrational energy to the solvent, it is irreversible.
18 Fluorescence spectrometer Two types of spectra: Fluorescence spectrum select excitation λ with mono #1 scan mono #2 to measure fluorescence spectrum Excitation spectrum - select fluorescence λ with mono #2 - scan excitation λ using mono #1. Under what conditions will the excitation spectrum resemble the absorption spectrum?
19 Excitation Spectroscopy py Absorption is a DIRECT technique for measuring an electronic transition the direct loss of transmitted light is measured. There are other techniques to infer the absorption of light: fluorescence excitation phosphorescence h excitation ti resonant ionisation photofragment excitation i
20 Explanation of fluorescence excitation Because the fluorescence is the same no matter where the molecule is excited, then any (or all) fluorescence transitions can be monitored. N (fluorescence photons) N (absorbed photons) N (fluorescence photons) = φ f x N (absorbed photons) The proportionality constant, φ f is called the fluorescence quantum yield φ f can vary between 0 and 1 If φ f is constant with λ, then fluorescence excitation spectrum has the same shape (intensity vs λ) as the absorption spectrum
21 Comparison of Absorption and Excitation Spectra Note the extended π chromophore, which is responsible for the absorption. Multiple electronic states 1500 Excitation Absorption Rhodamine 6G Wavelength (nm)
22 When absorption excitation 2000 Especially note this difference?? Pyrenesulfonic acid Wavelength (nm)
23 More on Internal Conversion IC S 2 IC Smaller energy gap S 1 Larger energy gap IC is usually very fast between excited states and slower between S 1 and the ground state. 1 g S 0 Internal conversion is the relaxation of the electron to a lower level, but, accompanied by no radiation, the equivalent amount of energy is converted to vibrational energy. IC is a radiationless process
24 Intersystem Crossing (ISC) and Phosphorescence ISC S 2 T 2 S 1 NRD & IC ISC T 1 Phosphorescenceh S 0 Intersystem crossing isthe flipping of an electron spin so that the molecule changes Intersystem crossing is the flipping of an electron spin so that the molecule changes from singlet to triplet state (or vice versa). ISC is a non radiative process and is typically 10 6 times slower than IC, other things being equal.
25 More on phosphorescence Triplet states S 2 T 2 S 1 Fluore escence T 1 Phosphorescenceh S 0 The lowest triplet is nearly always below the first excited singlet state. Therefore phosphorescence is nearly always red shifted (i.e. at lower energy) than fluorescence. It is formally forbidden and about 10 6 times slower than fluorescence.
26 Everything together IC ISC IC S 2 ISC T 2 S 1 Fluores scence Abso orption ISC Phosphorescence T 1 S 0
27 Phosphorescence in research Absorption spectrum phosphorescence S 2 S 0 S 1 SS 0 This porphyrin molecule exhibits a huge π-system and absorbs across the visible region. The palladium metal centre promotes intersystem crossing. The molecule was synthesized in the Crossley group, and used by Schmidt s group in solar research.
28 Ultrafast fluorescence Using ultrafast lasers, we can observe the porphyrin in the act of fluorescing. Here, after excitation at 300 nm, fluorescence at 680 nm builds up within 3 ps but gone after only 20 ps after which the molecules which are left are all in the T 1 state and then phosphoresce on the 20 μs timescale.
29 How fast? One picosecond is one millionth of one millionth of a second. There are as many picoseconds in a second as there have been seconds since our species invented shoes, years ago. The bisporphyrin below has a very low S 1 state. Ultrafast transient absorption experiments show that it does not undergo ISC, but rather IC is complete in 50ps. This experiment is 2 weeks old. S 1 S 0
30 Summary Emission always occurs from the lowest excited electronic state (Kasha s law) Internal conversion (IC) )is the non radiative i change process by which an electron relaxes to a lower energy state with the energyconverted to vibrational energy Fluorescence quantum yield, φ f, is the proportionality constant linking the number of photons emitted by fluorescence to the number absorbed If φ f is constant with the wavelength of the excitation, the φ f g, fluorescence spectrum has the same shape as the absorption spectrum Intersystem crossing is the non radiative change process by which a molecule changes spin Phosphorescence normally occurs from the lowest triplet state and is nearly always red shifted and slower than fluorescence.
31 Next lecture Course wrap up Week 13 homework Electronicspectroscopyworksheet in the tutorials Complete the practice problems at the end of the lectures Note: ALL of the relevant past exam problems have been used as practice problems. Other questions on past papers include parts which are no longer part of the course.
32 Practice Questions 1. The figure opposite shows the absorption, fluorescence and phosphorescence spectra of a common organic dye. Why is the phosphorescence p spectrum significantly red shifted compared to the fluorescence spectrum? 2. The spectra below show the fluorescence excitation (blue) and fluorescence emission spectrum (red) of two large molecules. Explain, the following features of the spectra, using a Jablonski diagram to illustrate your answer. (a) The Stokes shift is quite different for molecule A and B. Explain how this dff difference arises, and give an example of what molecular property might give rise to a large Stokes shift. (b) Molecule B in particular is a nice example of mirror symmetry between excitation and emission spectra. How does this mirror symmetry arise?
33 Practice Questions 3. The two spectra below show the fluorescence excitation and absorption spectra of pyrenesulfonic acid. (a) In addition to the identified electronic origin transitions, there are other peaks in the absorption spectrum, as indicated by a) in the figure. Using a Jablonski diagram, explain how these other peaks arise. (b) In the absorption spectrum, for the S 1 and S 2 transitions, the origin band is stronger than the two satellite bands marked by a). In the S 3 transition, the origin band is weaker than the satellite, marked by b. Explain, using the Franck Condon principle, how this arises. (c) Using a Jablonski diagram, describe one such process that can give rise to the observed difference in the relative intensities of the fluorescence excitation and absorption spectra at λ < 245 nm
Assumed knowledge. Chemistry 2. Learning outcomes. Electronic spectroscopy of polyatomic molecules. Franck-Condon Principle (reprise)
Chemistry 2 Lecture 11 Electronic spectroscopy of polyatomic molecules Assumed knowledge For bound excited states, transitions to the individual vibrational levels of the excited state are observed with
More informationWhat the Einstein Relations Tell Us
What the Einstein Relations Tell Us 1. The rate of spontaneous emission A21 is proportional to υ 3. At higher frequencies A21 >> B(υ) and all emission is spontaneous. A 21 = 8π hν3 c 3 B(ν) 2. Although
More informationIntroduction to Fluorescence Spectroscopies I. Theory
March 22, 2006; Revised January 26, 2011 for CHMY 374 Adapted for CHMY 362 November 13, 2011 Edited by Lauren Woods December 2, 2011 17mar14, P.Callis; 1feb17 P. Callis, 29jan18 P. Callis Introduction
More informationSinglet. Fluorescence Spectroscopy * LUMO
Fluorescence Spectroscopy Light can be absorbed and re-emitted by matter luminescence (photo-luminescence). There are two types of luminescence, in this discussion: fluorescence and phosphorescence. A
More informationExcited State Processes
Excited State Processes Photophysics Fluorescence (singlet state emission) Phosphorescence (triplet state emission) Internal conversion (transition to singlet gr. state) Intersystem crossing (transition
More informationLABORATORY OF ELEMENTARY BIOPHYSICS
LABORATORY OF ELEMENTARY BIOPHYSICS Experimental exercises for III year of the First cycle studies Field: Applications of physics in biology and medicine Specialization: Molecular Biophysics Fluorescence
More informationUV-vis (Electronic) Spectra Ch.13 Atkins, Ch.19 Engel
XV 74 UV-vis (Electronic) Spectra-2014 -Ch.13 Atkins, Ch.19 Engel Most broadly used analytical tech / especially bio-applic. inexpensive optics / solvent & cell usually not problem intense transitions
More informationChapter 17: Fundamentals of Spectrophotometry
Chapter 17: Fundamentals of Spectrophotometry Spectroscopy: the science that deals with interactions of matter with electromagnetic radiation or other forms energy acoustic waves, beams of particles such
More informationCHAPTER 13 Molecular Spectroscopy 2: Electronic Transitions
CHAPTER 13 Molecular Spectroscopy 2: Electronic Transitions I. General Features of Electronic spectroscopy. A. Visible and ultraviolet photons excite electronic state transitions. ε photon = 120 to 1200
More informationChapter 17: Fundamentals of Spectrophotometry
Chapter 17: Fundamentals of Spectrophotometry Spectroscopy: the science that deals with interactions of matter with electromagnetic radiation or other forms energy acoustic waves, beams of particles such
More informationFluorescence (Notes 16)
Fluorescence - 2014 (Notes 16) XV 74 Jablonski diagram Where does the energy go? Can be viewed like multistep kinetic pathway 1) Excite system through A Absorbance S 0 S n Excite from ground excited singlet
More informationChapter 6 Photoluminescence Spectroscopy
Chapter 6 Photoluminescence Spectroscopy Course Code: SSCP 4473 Course Name: Spectroscopy & Materials Analysis Sib Krishna Ghoshal (PhD) Advanced Optical Materials Research Group Physics Department, Faculty
More informationMolecular Luminescence. Absorption Instrumentation. UV absorption spectrum. lg ε. A = εbc. monochromator. light source. Rotating mirror (beam chopper)
Molecular Luminescence Absorption Instrumentation light source I 0 sample I detector light source Rotating mirror (beam chopper) motor b sample I detector reference I 0 UV absorption spectrum lg ε A =
More informationPHOTOCHEMISTRY NOTES - 1 -
- 1 - PHOTOCHEMISTRY NOTES 1 st Law (Grotthus-Draper Law) Only absorbed radiation produces chemical change. Exception inelastic scattering of X- or γ-rays (electronic Raman effect). 2 nd Law (Star-Einstein
More informationCD Basis Set of Spectra that is used is that derived from comparing the spectra of globular proteins whose secondary structures are known from X-ray
CD Basis Set of Spectra that is used is that derived from comparing the spectra of globular proteins whose secondary structures are known from X-ray crystallography An example of the use of CD Modeling
More informationChapter 15 Molecular Luminescence Spectrometry
Chapter 15 Molecular Luminescence Spectrometry Two types of Luminescence methods are: 1) Photoluminescence, Light is directed onto a sample, where it is absorbed and imparts excess energy into the material
More informationLecture 3: Light absorbance
Lecture 3: Light absorbance Perturbation Response 1 Light in Chemistry Light Response 0-3 Absorbance spectrum of benzene 2 Absorption Visible Light in Chemistry S 2 S 1 Fluorescence http://www.microscopyu.com
More informationMolecular Luminescence Spectroscopy
Molecular Luminescence Spectroscopy In Molecular Luminescence Spectrometry ( MLS ), molecules of the analyte in solution are excited to give a species whose emission spectrum provides information for qualitative
More informationFluorescence Excitation and Emission Fundamentals
Fluorescence Excitation and Emission Fundamentals Fluorescence is a member of the ubiquitous luminescence family of processes in which susceptible molecules emit light from electronically excited states
More informationXV 74. Flouorescence-Polarization-Circular-Dichroism- Jablonski diagram Where does the energy go?
XV 74 Flouorescence-Polarization-Circular-Dichroism- Jablonski diagram Where does the energy go? 1) Excite system through A Absorbance S 0 S n Excite from ground excited singlet S = 0 could be any of them
More informationReflection = EM strikes a boundary between two media differing in η and bounces back
Reflection = EM strikes a boundary between two media differing in η and bounces back Incident ray θ 1 θ 2 Reflected ray Medium 1 (air) η = 1.00 Medium 2 (glass) η = 1.50 Specular reflection = situation
More informationsingle-molecule fluorescence resonance energy transfer
single-molecule fluorescence resonance energy transfer (2) determing the Förster radius: quantum yield, donor lifetime, spectral overlap, anisotropy michael börsch 26/05/2004 1 fluorescence (1) absorbance
More informationNPTEL/IITM. Molecular Spectroscopy Lectures 1 & 2. Prof.K. Mangala Sunder Page 1 of 15. Topics. Part I : Introductory concepts Topics
Molecular Spectroscopy Lectures 1 & 2 Part I : Introductory concepts Topics Why spectroscopy? Introduction to electromagnetic radiation Interaction of radiation with matter What are spectra? Beer-Lambert
More informationFluorescence Polarization Anisotropy FPA
Fluorescence Polarization Anisotropy FPA Optics study of light Spectroscopy = light interacts the study of the interaction between matter & electro-magnetic radiation matter Spectroscopy Atomic Spectroscopy
More informationCHEM Outline (Part 15) - Luminescence 2013
CHEM 524 -- Outline (Part 15) - Luminescence 2013 XI. Molecular Luminescence Spectra (Chapter 15) Kinetic process, competing pathways fluorescence, phosphorescence, non-radiative decay Jablonski diagram
More informationLuminescence. Photoluminescence (PL) is luminescence that results from optically exciting a sample.
Luminescence Topics Radiative transitions between electronic states Absorption and Light emission (spontaneous, stimulated) Excitons (singlets and triplets) Franck-Condon shift(stokes shift) and vibrational
More information1 Basic Optical Principles
13 1 Basic Optical Principles 1.1 Introduction To understand important optical methods used to investigate biomolecules, such as fluorescence polarization anisotropy, Förster resonance energy transfer,
More informationChemistry Instrumental Analysis Lecture 3. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 3 Quantum Transitions The energy of a photon can also be transferred to an elementary particle by adsorption if the energy of the photon exactly matches the
More informationAdvanced Organic Chemistry Chm 512/412 Spring Handout I Photochemistry Part 1. Photophysical Processes Quenching Alkene cis-trans Isomerization
Advanced rganic Chemistry Chm 512/412 Spring 2010 Handout I Photochemistry Part 1 Photophysical Processes Quenching Alkene cis-trans Isomerization Importance of Photochemistry/Photophysics rganic Synthesis
More informationFigure 1 Relaxation processes within an excited state or the ground state.
Excited State Processes and Application to Lasers The technology of the laser (Light Amplified by Stimulated Emission of Radiation) was developed in the early 1960s. The technology is based on an understanding
More informationDepartment of Chemistry Physical Chemistry Göteborg University
Department of Chemistry Physical Chemistry Göteborg University &RQVWUXFWLRQRIDSXOVHGG\HODVHU 3OHDVHREVHUYHWKDWWKHVDIHW\SUHFDXWLRQVRQSDJHPXVW EHIROORZHGRWKHUZLVHWKHUHLVDULVNRIH\HGDPDJH Renée Andersson -97,
More informationModel Answer (Paper code: AR-7112) M. Sc. (Physics) IV Semester Paper I: Laser Physics and Spectroscopy
Model Answer (Paper code: AR-7112) M. Sc. (Physics) IV Semester Paper I: Laser Physics and Spectroscopy Section I Q1. Answer (i) (b) (ii) (d) (iii) (c) (iv) (c) (v) (a) (vi) (b) (vii) (b) (viii) (a) (ix)
More informationExcited States in Organic Light-Emitting Diodes
Excited States in Organic Light-Emitting Diodes The metal-to-ligand charge transfer (MLCT) excited states of d 6 π coordination compounds have emerged as the most efficient for solar harvesting and sensitization
More informationElectronic Spectra of Complexes
Electronic Spectra of Complexes Interpret electronic spectra of coordination compounds Correlate with bonding Orbital filling and electronic transitions Electron-electron repulsion Application of MO theory
More informationChem 442 Review of Spectroscopy
Chem 44 Review of Spectroscopy General spectroscopy Wavelength (nm), frequency (s -1 ), wavenumber (cm -1 ) Frequency (s -1 ): n= c l Wavenumbers (cm -1 ): n =1 l Chart of photon energies and spectroscopies
More informationModern Optical Spectroscopy
Modern Optical Spectroscopy With Exercises and Examples from Biophysics and Biochemistry von William W Parson 1. Auflage Springer-Verlag Berlin Heidelberg 2006 Verlag C.H. Beck im Internet: www.beck.de
More informationLecture 8. Assumed knowledge
Chemistry 2 Lecture 8 IR Spectroscopy of Polyatomic Molecles Assumed knowledge There are 3N 6 vibrations in a non linear molecule and 3N 5 vibrations in a linear molecule. Only modes that lead to a change
More informationChemistry 2. Assumed knowledge
Chemistry 2 Lecture 8 IR Spectroscopy of Polyatomic Molecles Assumed knowledge There are 3N 6 vibrations in a non linear molecule and 3N 5 vibrations in a linear molecule. Only modes that lead to a change
More informationLuminescence spectroscopy
Febr. 203 Luminescence spectroscopy Biophysics 2 nd semester Józse Orbán University o Pécs, Department o Biophysics Deinitions, laws FUNDAMENTALS o SPECTROSCY review - Spectral types (absorbtion/emission
More informationGeneration of light Light sources
Generation of light Light sources Black-body radiation Luminescence Luminescence Laser Repetition Types of energy states in atoms and molecules are independent (not coupled) Energy states are non-continuous,
More informationWhat dictates the rate of radiative or nonradiative excited state decay?
What dictates the rate of radiative or nonradiative excited state decay? Transitions are faster when there is minimum quantum mechanical reorganization of wavefunctions. This reorganization energy includes
More informationtwo slits and 5 slits
Electronic Spectroscopy 2015January19 1 1. UV-vis spectrometer 1.1. Grating spectrometer 1.2. Single slit: 1.2.1. I diffracted intensity at relative to un-diffracted beam 1.2.2. I - intensity of light
More informationBecause light behaves like a wave, we can describe it in one of two ways by its wavelength or by its frequency.
Light We can use different terms to describe light: Color Wavelength Frequency Light is composed of electromagnetic waves that travel through some medium. The properties of the medium determine how light
More information1. Transition dipole moment
1. Transition dipole moment You have measured absorption spectra of aqueous (n=1.33) solutions of two different chromophores (A and B). The concentrations of the solutions were the same. The absorption
More informationChap. 12 Photochemistry
Chap. 12 Photochemistry Photochemical processes Jablonski diagram 2nd singlet excited state 3rd triplet excited state 1st singlet excited state 2nd triplet excited state 1st triplet excited state Ground
More informationLinear and nonlinear spectroscopy
Linear and nonlinear spectroscopy We ve seen that we can determine molecular frequencies and dephasing rates (for electronic, vibrational, or spin degrees of freedom) from frequency-domain or timedomain
More information10. 6 Photochemistry. Out-class reading: Levine, pp photochemistry
Out-class reading: Levine, pp. 800-804 photochemistry 6.1 Brief introduction of light 1) Photochemistry The branch of chemistry which deals with the study of chemical reaction initiated by light. 2) Energy
More informationFluorescence 2009 update
XV 74 Fluorescence 2009 update Jablonski diagram Where does the energy go? Can be viewed like multistep kinetic pathway 1) Excite system through A Absorbance S 0 S n Excite from ground excited singlet
More informationSpectroscopy Problem Set February 22, 2018
Spectroscopy Problem Set February, 018 4 3 5 1 6 7 8 1. In the diagram above which of the following represent vibrational relaxations? 1. Which of the following represent an absorbance? 3. Which of following
More informationIR Spectrography - Absorption. Raman Spectrography - Scattering. n 0 n M - Raman n 0 - Rayleigh
RAMAN SPECTROSCOPY Scattering Mid-IR and NIR require absorption of radiation from a ground level to an excited state, requires matching of radiation from source with difference in energy states. Raman
More informationChemistry 304B, Spring 1999 Lecture 5 1. UV Spectroscopy:
Chemistry 304B, Spring 1999 Lecture 5 1 Ultraviolet spectroscopy; UV Spectroscopy: Infrared spectroscopy; Nuclear magnetic resonance spectroscopy General basis of spectroscopy: Shine light at a collection
More informationGeneral Considerations 1
General Considerations 1 Absorption or emission of electromagnetic radiation results in a permanent energy transfer from the emitting object or to the absorbing medium. This permanent energy transfer can
More informationFluorescence Spectroscopy
Fluorescence Spectroscopy Frequency and time dependent emission Emission and Excitation fluorescence spectra Stokes Shift: influence of molecular vibrations and solvent Time resolved fluorescence measurements
More informationLast Updated: April 22, 2012 at 7:49pm
Page 1 Electronic Properties of d 6 π Coordination Compounds The metal-to-ligand charge transfer (MLCT) excited states of d 6 π coordination compounds have emerged as the most efficient for both solar
More informationChemistry 524--Final Exam--Keiderling May 4, :30 -?? pm SES
Chemistry 524--Final Exam--Keiderling May 4, 2011 3:30 -?? pm -- 4286 SES Please answer all questions in the answer book provided. Calculators, rulers, pens and pencils are permitted. No open books or
More informationLab 11: Must what goes in be the same as what comes out? Spectroscopy & Fluorescence in Chlorophyll.
Lab 11: Must what goes in be the same as what comes out? Spectroscopy & Fluorescence in Chlorophyll. Introduction to Fluorescence: Fluorescence is one of the possible mechanisms for emission of light by
More informationOrganic Photochemistry and Pericyclic Reactions Prof. N.D. Pradeep Singh Department of Chemistry Indian Institute of Technology Kharagpur
Organic Photochemistry and Pericyclic Reactions Prof. N.D. Pradeep Singh Department of Chemistry Indian Institute of Technology Kharagpur Lecture No. #01 Introduction to Organic Photochemistry (Refer Slide
More informationComments to Atkins: Physical chemistry, 7th edition.
Comments to Atkins: Physical chemistry, 7th edition. Chapter 16: p. 483, Eq. (16.1). The definition that the wave number is the inverse of the wave length should be used. That is much smarter. p. 483-484.
More informationChapter 3. Electromagnetic Theory, Photons. and Light. Lecture 7
Lecture 7 Chapter 3 Electromagnetic Theory, Photons. and Light Sources of light Emission of light by atoms The electromagnetic spectrum see supplementary material posted on the course website Electric
More informationChemistry Instrumental Analysis Lecture 5. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 5 Light Amplification by Stimulated Emission of Radiation High Intensities Narrow Bandwidths Coherent Outputs Applications CD/DVD Readers Fiber Optics Spectroscopy
More information1. Photoreduction of Benzophenone in 2-Propanol
1. Photoreduction of Benzophenone in 2-Propanol Topic: photochemistry, photophysics, kinetics, physical-organic chemistry Level: undergraduate physical chemistry Time: 2 x 2 hours (separated by ~24 hours)
More informationProblem 1. Anthracene and a chiral derivative of anthracene
Molecular Photophysics 330 Physical rganic Chemistry 6C50 Thursday November 5 004, 4.00-7.00 h This exam consists of four problems that have an equal weight in the final score Most problems are composed
More informationFluorescence Workshop UMN Physics June 8-10, 2006 Basic Spectroscopic Principles Joachim Mueller
Fluorescence Workshop UMN Physics June 8-10, 2006 Basic Spectroscopic Principles Joachim Mueller Fluorescence, Light, Absorption, Jablonski Diagram, and Beer-Law First stab at a definition: What is fluorescence?
More informationSchool of Chemistry, University of Sydney, Sydney, New South Wales 2006, Australia
CHAPTER 1 Basics of Fluorescence ROBERT P. LEARMONTH Department of Biological and Physical Sciences and Centre for Systems Biology, University of Southern Queensland, Toowoomba, Queensland 4350, Australia
More informationChem Homework Set Answers
Chem 310 th 4 Homework Set Answers 1. Cyclohexanone has a strong infrared absorption peak at a wavelength of 5.86 µm. (a) Convert the wavelength to wavenumber.!6!1 8* = 1/8 = (1/5.86 µm)(1 µm/10 m)(1 m/100
More informationPhotochemical principles
Chapter 1 Photochemical principles Dr. Suzan A. Khayyat 1 Photochemistry Photochemistry is concerned with the absorption, excitation and emission of photons by atoms, atomic ions, molecules, molecular
More informationIntroduction ENERGY. Heat Electricity Electromagnetic irradiation (light)
Photochemistry Introduction ENERGY Heat Electricity Electromagnetic irradiation (light) Vision: Triggered by a photochemical reaction Is red in the dark? The answer must be NO - Since what we see as colour
More informationwbt Λ = 0, 1, 2, 3, Eq. (7.63)
7.2.2 Classification of Electronic States For all diatomic molecules the coupling approximation which best describes electronic states is analogous to the Russell- Saunders approximation in atoms The orbital
More informationn ( λ ) is observed. Further, the bandgap of the ZnTe semiconductor is
Optical Spectroscopy Lennon O Naraigh, 0000 Date of Submission: 0 th May 004 Abstract: This experiment is an exercise in the principles and practice of optical spectroscopy. The continuous emission spectrum
More informationSpectroscopy. Page 1 of 8 L.Pillay (2012)
Spectroscopy Electromagnetic radiation is widely used in analytical chemistry. The identification and quantification of samples using electromagnetic radiation (light) is called spectroscopy. Light has
More informationMolecular spectroscopy
Molecular spectroscopy Origin of spectral lines = absorption, emission and scattering of a photon when the energy of a molecule changes: rad( ) M M * rad( ' ) ' v' 0 0 absorption( ) emission ( ) scattering
More informationLecture 0. NC State University
Chemistry 736 Lecture 0 Overview NC State University Overview of Spectroscopy Electronic states and energies Transitions between states Absorption and emission Electronic spectroscopy Instrumentation Concepts
More informationChapter 11. Basics in spin-orbit couplings
1- The Jablonski diagram (or the state diagram of diamagnetic molecules) 2- Various natures of excited states and basics in molecular orbitals 3- Vibronic coupling and the Franck-Condon term 4- Excited
More informationRaman and stimulated Raman spectroscopy of chlorinated hydrocarbons
Department of Chemistry Physical Chemistry Göteborg University KEN140 Spektroskopi Raman and stimulated Raman spectroscopy of chlorinated hydrocarbons WARNING! The laser gives a pulsed very energetic and
More informationWhat is spectroscopy?
Absorption Spectrum What is spectroscopy? Studying the properties of matter through its interaction with different frequency components of the electromagnetic spectrum. With light, you aren t looking directly
More information24/ Rayleigh and Raman scattering. Stokes and anti-stokes lines. Rotational Raman spectroscopy. Polarizability ellipsoid. Selection rules.
Subject Chemistry Paper No and Title Module No and Title Module Tag 8/ Physical Spectroscopy 24/ Rayleigh and Raman scattering. Stokes and anti-stokes lines. Rotational Raman spectroscopy. Polarizability
More informationLaser induced fluorescence
Report experiment 1 by 4-7 February 22, id#471917 Summary Several measurements are made around laser induced fluorescence (LIF). First the system was calibrated. The resolution of the spectrograph was
More informationC101-E145 TALK LETTER. Vol. 17
C101-E145 TALK LETTER Vol. 17 UV-VIS Spectroscopy and Fluorescence Spectroscopy (Part 1 of 2) ------- 02 Applications: Spectrofluorophotometers Used in a Variety of Fields ------- 06 Q&A: Is there a way
More informationQuantum Chemistry. NC State University. Lecture 5. The electronic structure of molecules Absorption spectroscopy Fluorescence spectroscopy
Quantum Chemistry Lecture 5 The electronic structure of molecules Absorption spectroscopy Fluorescence spectroscopy NC State University 3.5 Selective absorption and emission by atmospheric gases (source:
More informationWavelength λ Velocity v. Electric Field Strength Amplitude A. Time t or Distance x time for 1 λ to pass fixed point. # of λ passing per s ν= 1 p
Introduction to Spectroscopy (Chapter 6) Electromagnetic radiation (wave) description: Wavelength λ Velocity v Electric Field Strength 0 Amplitude A Time t or Distance x Period p Frequency ν time for 1
More informationIdentification of ultrafast processes in ZnPc by pump-probe spectroscopy
Identification of ultrafast processes in ZnPc by pump-probe spectroscopy S Ombinda-Lemboumba 1,2,4, A du Plessis 1,2,3, C M Steenkamp 2, L R Botha 1,2 and E G Rohwer 2 1 CSIR National Laser Centre, Pretoria,
More informationFluorescence spectra of 1-Pyrenebutyric acid, concentration dependence and dynamic quenching phenomena
Practicum Spectroscopy Fall 2010 Fluorescence spectra of 1-Pyrenebutyric acid, concentration dependence and dynamic quenching phenomena LUM Jorge Ferreiro, study degree Chemistry, 5 th semester, fjorge@student.ethz.ch
More informationELECTRONIC SPECTROSCOPY AND PHOTOCHEMISTRY
5.61 Physical Chemistry Lecture #33 1 ELECTRONIC SPECTROSCOPY AND PHOTOCHEMISTRY The ability of light to induce electronic transitions is one of the most fascinating aspects of chemistry. It is responsible
More informationToday: general condition for threshold operation physics of atomic, vibrational, rotational gain media intro to the Lorentz model
Today: general condition for threshold operation physics of atomic, vibrational, rotational gain media intro to the Lorentz model Laser operation Simplified energy conversion processes in a laser medium:
More informationDetermining Riboflavin Content in a Multivitamin: Things to do before the lab
Determining Riboflavin Content in a Multivitamin: Things to do before the lab Calculate everything you can that you can in advance Step 1: Calculate the dilution of a 4.0 M solution to 1 L of a 0.02 M
More informationAtomic Spectra for Atoms and Ions. Light is made up of different wavelengths
Atomic Spectra for Atoms and Ions What will you be doing in lab next week? Recording the line spectra of several different substances in discharge tubes. Recording the line spectra of several ions from
More informationOptics and Spectroscopy
Introduction to Optics and Spectroscopy beyond the diffraction limit Chi Chen 陳祺 Research Center for Applied Science, Academia Sinica 2015Apr09 1 Light and Optics 2 Light as Wave Application 3 Electromagnetic
More information9. Transitions between Magnetic Levels Spin Transitions Between Spin States. Conservation of Spin Angular Momentum
9. Transitions between Magnetic Levels pin Transitions Between pin tates. Conservation of pin Angular Momentum From the magnetic energy diagram derived in the previous sections (Figures 14, 15 and 16),
More information16. Reactions of the Radical Pairs. The I P Step of the Paradigm
16. Reactions of the Radical Pairs. The I P Step of the Paradigm Let us consider the product forming step in the general photochemical paradigm, i.e., the I P process shown in Figure 1. From the discussion
More informationWeek 6: Ch. 8 Scintillation Counters
Week 6: Ch. 8 cintillation Counters Proportional Counters Principles of cintillation Counters -- organic materials --- light production -- inorganic materials --- light production -- light output, collection
More informationFluorescence Quenching
Summary Fluorescence Quenching The emission of light from the excited state of a molecule (fluorescence or phosphorescence) can be quenched by interaction with another molecule. The stationary and time-dependent
More informationUltraviolet-Visible and Infrared Spectrophotometry
Ultraviolet-Visible and Infrared Spectrophotometry Ahmad Aqel Ifseisi Assistant Professor of Analytical Chemistry College of Science, Department of Chemistry King Saud University P.O. Box 2455 Riyadh 11451
More informationChapter 13. Phys 322 Lecture 34. Modern optics
Chapter 13 Phys 3 Lecture 34 Modern optics Blackbodies and Lasers* Blackbodies Stimulated Emission Gain and Inversion The Laser Four-level System Threshold Some lasers Pump Fast decay Laser Fast decay
More informationLaser Induced Fluorescence of Iodine
Laser Induced Fluorescence of Iodine (Last revised: FMH 29 Sep 2009) 1. Introduction In this experiment we are going to study the laser induced fluorescence of iodine in the gas phase. The aim of the study
More informationChapter 5 Light and Matter: Reading Messages from the Cosmos. How do we experience light? Colors of Light. How do light and matter interact?
Chapter 5 Light and Matter: Reading Messages from the Cosmos How do we experience light? The warmth of sunlight tells us that light is a form of energy We can measure the amount of energy emitted by a
More information6.05 Computational Raman Spectroscopy
2nd/3rd year Physical Chemistry Practical Course, Oxford University 6.05 Computational Raman Spectroscopy (5 points) Raman spectra are obtained by irradiating a sample with very intense monochromatic radiation,
More information24 Introduction to Spectrochemical Methods
24 Introduction to Spectrochemical Methods Spectroscopic method: based on measurement of the electromagnetic radiation produced or absorbed by analytes. electromagnetic radiation: include γ-ray, X-ray,
More informationPART VI : MOLECULAR LUMINESCENCE SPECTROSCOPY (Recommendations 1985)
PART VI : MOLECULAR LUMINESCENCE SPECTROSCOPY (Recommendations 1985) 1. INTRODUCTION This document does not aim to be completely self-contained since many of the terms and units needed for describing Molecular
More informationChem 344 Final Exam Tuesday, Dec. 11, 2007, 3-?? PM
Chem 344 Final Exam Tuesday, Dec. 11, 2007, 3-?? PM Closed book exam, only pencils and calculators permitted. You may bring and use one 8 1/2 x 11" paper with anything on it. No Computers. Put all of your
More informationLASER. Light Amplification by Stimulated Emission of Radiation
LASER Light Amplification by Stimulated Emission of Radiation Laser Fundamentals The light emitted from a laser is monochromatic, that is, it is of one color/wavelength. In contrast, ordinary white light
More information