Other hypotheses of interest (cont d)
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1 Other hypotheses of interest (cont d) In addition to the simple null hypothesis of no treatment effects, we might wish to test other hypothesis of the general form (examples follow): H 0 : C k g β g p = 0, Comparisons among treatments H 0 : β g p M p q = 0, Comparisons across traits H 0 : C k g β g p M p q = 0, A combination of both. For example, suppose that we measure p = 2 traits on each unit in g = 3 treatment groups, and we set τ 3 = [τ 31 τ 32 ] = 0. In this case, β = µ 1 µ 2 τ 11 τ 12 τ 21 τ
2 Other hypotheses of interest (cont d) Comparisons of treatment means: Cβ = [ ] µ 1 µ 2 τ 11 τ 12 τ 21 τ 22 = [ τ 11 τ 12 τ 11 τ 21 τ 12 τ 22 ]. Under the restriction τ 13 = τ 23 = 0, the first row of β is the expected value of units in treatment 3. First row of C provides differences between mean resposnes for treatments 3 and 1 for both traits. Second row of C provides differences between mean responses for treatments 1 and 2 for both traits. 390
3 Other hypotheses of interest (cont d) Comparison of trait means with each treatment group: βm = µ 1 µ 2 τ 11 τ 12 τ 21 τ 22 [ 1 1 ] = µ 1 µ 2 τ 11 τ 12 τ 21 τ 22 The first row compares mean responses for trait 1 and trait 2 for units receiving treatment 3. The second row compares mean responses for trait 1 and trait 2 for units receiving treatment 1. The third row compares mean responses for trait 1 and trait 2 for units receiving treatment
4 Other hypotheses of interest (cont d) One might be interested in comparing the effects of the treatments on just the first trait: CβM = [ ] µ 1 µ 2 τ 11 τ 12 τ 21 τ 22 [ 1 0 ] = [ τ 11 τ 11 τ 21 ]. Row 1 is the difference of the effects of treatments 3 and 1 on mean responses for trait 1. Row 2 is the difference of the effect of treatments 1 and 2 on mean responses for trait
5 Other hypotheses of interest (cont d) CβM = One might be interested in interaction contrasts : [ ] µ 1 µ 2 τ 11 τ 12 τ 21 τ 22 [ 1 1 ] = [ τ 11 τ 21 (τ 11 τ 21 ) (τ 12 τ 22 ) ]. The first is the difference in mean responses to treatments 3 and 1 for trait 1 minus the difference in mean responses to treatments 3 and 1 for trait 2. The second is the difference in mean responses to treatments 1 and 2 for trait 1 minus the difference in mean responses to treatments 1 and 2 for trait
6 F approximation to the sampling distribution of Wilk s Criterion C. R. Rao (1951) Bulletin Int. Stat. Inst. 33(2), Coincides with exact F-distribution for cases described on a previous slide More accurate than large sample chi-square approximation Similar F-approximations are used by SAS for Pillai s trace and the Lawley-Hotelling trace. 394
7 F approximation to the sampling distribution of Wilk s Criterion When H 0 : C k r β r p M p u = 0 k u is true where 1 Λ 1/b ab c Λ 1/b uk F (ku,ab c) a = (n r) (u k + 1)/2 b = u2 k 2 4 u 2 + k 2 5 c = (uk 2)/2 395
8 Example: One-way MANOVA Populations: g = 3 types of students (k = 1) Technical school students (n 1 = 23) (k = 2) Architecture students (n 2 = 38) (k = 3) Medical technology students (n 3 = 21) Response variables: p = 4 test scores aptitude test mathematics test language test general knowledge 396
9 One-way MANOVA model where X ij = Example (cont d) X ij1 X ij2 X ij3 X ij4 ɛ ij = = ɛ ij1 ɛ ij2 ɛ ij3 ɛ ij4 µ 1 µ 2 µ 3 µ 4 + τ i1 τ i2 τ i3 τ i4 + NID(0, Σ) ɛ ij1 ɛ ij2 ɛ ij3 ɛ ij4 and use the SAS constraints τ 31 = τ 32 = τ 33 = τ 34 = 0 397
10 Example (cont d) Test the null hypothesis that the mean vectors for the four traits are the same for all three types of students Write the one-way MANOVA model in matrix form X n p = A n r β r p + ɛ n p where β 3 4 = µ 1 µ 2 µ 3 µ 4 τ 11 τ 12 τ 13 τ 14 τ 21 τ 22 τ 23 τ
11 Example (cont d) The null hypothesis that the mean vectors for the four traits are the same for all three types of students can be written as H 0 : Cβ = [ ] µ 1 µ 2 µ 3 µ 4 τ 11 τ 12 τ 13 τ 14 τ 21 τ 22 τ 23 τ 24 Here k = 2 rows in C r = 3 rows in β p = 4 response variables M = I 4 4 so u = p = 4 n = n 1 + n 2 + n 3 = = 82 = [ ] 399
12 The value of Wilks Lambda is and Example (cont d) W B+W = a = (82 3) ( )/2 = 77.5 b = = 2 c = ((4)(2) 2)/2 = 3 F = ( 1 Λ Λ ) (ab c uk ) = 6.76 on (uk, ab c) = (8, 152) degrees of freedom and p-value <.0001 Conclude that the means scores are different for at least two types of students for at least one the the four response variables 400
13 Example (Cont d) Can test the null hypothesis of equal response means across populations by running a one way ANOVA for each of the four response variables. SAS code (in morel.sas) PROC GLM DATA=SET1; CLASS GROUP; MODEL X1-X4 = GROUP / Solution; MANOVA H=group /PRINTH PRINTE; RUN; 401
14 Example (Cont d) MANOVA Test Criteria and F Approximations for the Hypothesis of No Overall group Effect H = Type III SSCP Matrix for group E = Error SSCP Matrix S=2 M=0.5 N=37 Statistic Value F Value Num DF Den DF p-value Wilks Lambda <.0001 Pillai s Trace <.0001 Hotelling-Lawley Trace <.0001 Roy s Greatest Root NOTE: F Statistic for Roy s Greatest Root is an upper bound. NOTE: F Statistic for Wilks Lambda is exact. 402
15 Example (Cont d): morel.r > morel[,1] <- as.factor(morel[,1]) > fit <- manova(as.matrix(morel[,-1])~morel[,1]) > summary(fit, test="wilks") Df Wilks approx F num Df den Df Pr(>F) morel[, 1] e-07 *** Residuals Signif. codes: 0 *** ** 0.01 * > summary(fit, test="pillai") Df Pillai approx F num Df den Df Pr(>F) morel[, 1] e-07 *** Residuals
16 Bonferroni t-tests and intervals Can also examine Bonferroni simultaneous t-tests and CIs For the difference in treatment means for the i-th response (µ i + τ ki ) (µ i + τ li ) = τ ki τ li, we need the variance of X ki = X li : ( 1 Var(ˆτ ki ˆτ li ) = Var( X ki X li ) = + 1 σ ii, n k n l where σ ii is estimated as s pooled,ii = w ii n g, with w ii the ith diagonal element of the within-groups SSP matrix W. ) 404
17 Bonferroni t-tests and intervals If we wish to carry out all pairwise comparisons, there will be pg(g 1)/2 of them. To maintain a simultaneous type I error level of no more than α we can use Reject H 0 : τ ki = τ li if t n g ( α pg(g 1) ) where m =. 2m 2 t = X ki = X li ) > t n g ( α spooled,ii 2m ) ( 1nk + 1 nl 405
18 Bonferroni simultaneous intervals We have three groups and four variables, for a total of 4 3 2/2 = 12 comparisons, three for each response variable. From the output: w 11 = w 22 = w 33 = w 44 = with n 1 = 23, n 2 = 37, n 3 = 20 and n g = 82 3 =
19 Bonferroni simultaneous intervals A 95% confidence interval for the true difference between technical school and architecture students on mathematics is w 22 ( 1 x 12 x 22 ± t n g ( α 2m ) + 1 n g n 1 n ± t 79 (0.05/24) ± ( , 6.855). ) ( ) 37 Since the interval includes 0, we conclude that there is insufficient evidence to reject the null hypothesis of equal mathematics means scores for technical and architecture students. 407
20 Bonferroni simultaneous intervals We carry out similar calculations for other types of students. Compare technical school to medical technology students: w 22 ( 1 x 12 x 32 ± t n g ( α 2m ) + 1 n g n 1 n ± t 79 (0.05/24) ± ( 2.965, ). ) ( )
21 Bonferroni simultaneous intervals Architecture versus medical technology students: x 22 x 32 ± t n g ( α 2m ) w 22 n g ( ) 1 n n ± (2.381, ). Repeat these calculations for each of the other three response variables to construct 12 confidence intervals with simultaneous 95% confidence. 409
22 Test for Parallel Profiles Are the differences in mean scores for each pair of tests consistent across all 3 student groups? Are the differences in mean scores between two student groups consistent across all response variables? Parallel profiles are equivalent to no group test interaction 410
23 Test for Parallel Profiles The no group test interaction null hypothesis is expressed as [(µ 1 + τ 11 ) (µ 2 + τ 12 )] [µ 1 µ 2 ] = 0 [(µ 2 + τ 12 ) (µ 3 + τ 13 )] [µ 2 µ 3 ] = 0 [(µ 3 + τ 13 ) (µ 4 + τ 14 )] [µ 3 µ 4 ] = 0 [(µ 1 + τ 21 ) (µ 2 + τ 22 )] [µ 1 µ 2 ] = 0 [(µ 2 + τ 22 ) (µ 3 + τ 23 )] [µ 2 µ 3 ] = 0 [(µ 3 + τ 23 ) (µ 4 + τ 24 )] [µ 3 µ 4 ] = 0 411
24 This is equivalent to Test for Parallel Profiles H 0 : C k r β r p M p u = = [ [ ] µ 1 µ 2 µ 3 µ 4 τ 11 τ 12 τ 13 τ 14 τ 21 τ 22 τ 23 τ 24 ] where k = 2 rows in C r = 3 rows in β p = 4 response variables u = 3 n = n 1 + n 2 + n 3 = =
25 The value of Wilks Lambda is Test for Parallel Profiles W B+W = a = (82 3) ( )/2 = 78 b = = 2 c = ((3)(2) 2)/2 = 2 F = ( 1 Λ Λ ) (ab c uk ) = 8.36 on (uk, ab c) = (6, 154) degrees of freedom and p-value <.0001 Conclude that the mean score profiles are not parallel. For at least one pair of response variables, the difference in the mean responses is not the same for all types of students. 413
26 Testing for parallel profiles using R > M <- matrix(c(1, 0, 0, -1, 1, 0, 0, -1, 1, 0, 0, -1), ncol = 4) > fitp <- manova((as.matrix(morel[, -1]) %*% t(m)) ~ morel[, 1]) > summary(fitp, test="wilks") Df Wilks approx F num Df den Df Pr(>F) morel[, 1] e-08 *** Residuals Signif. codes: 0 *** ** 0.01 *
27 MANOVA Using the (preferred) car package > morel$studentgroup <- as.factor(morel$studentgroup) > library(car) > fit.lm <- lm(cbind(aptitude, mathematics, language, generalknowledge)~studentgroup, data = morel) > fit.manova <- Manova(fit.lm) > summary(fit.manova) Type II MANOVA Tests: Sum of squares and products for error: aptitude mathematics language generalknowledge aptitude mathematics language generalknowledge
28 Term: studentgroup Sum of squares and products for the hypothesis: aptitude mathematics language generalknowledge aptitude mathematics language generalknowledge Multivariate Tests: studentgroup Df test stat approx F num Df den Df Pr(>F) Pillai e-07 *** Wilks e-07 *** Hotelling-Lawley e-08 *** Roy e-08 *** 416
29 > fit$sspe aptitude mathematics language generalknowledge aptitude mathematics language generalknowledge > C <- matrix(c(0, 1, 0, 0, 1, -1), ncol = 3, by = T) > M <- matrix(c(1, 0, 0, -1, 1, 0, 0, -1, 1, 0, 0, -1), nrow = 4, by = T) > newfit <- linearhypothesis(model = fit.lm, hypothesis.matrix = C) > print(newfit) Multivariate Tests: Df test stat approx F num Df den Df Pr(>F) Pillai e-07 *** Wilks e-07 *** Hotelling-Lawley e-08 *** Roy e-08 *** 417
30 > newfit <- linearhypothesis(model = fit.lm, hypothesis.matrix = C, P = M) > print(newfit) Response transformation matrix: [,1] [,2] [,3] aptitude mathematics language generalknowledge
31 Sum of squares and products for the hypothesis: [,1] [,2] [,3] [1,] [2,] [3,] Sum of squares and products for error: [,1] [,2] [,3] [1,] [2,] [3,] Multivariate Tests: Df test stat approx F num Df den Df Pr(>F) Pillai e-07 *** Wilks e-08 *** Hotelling-Lawley e-08 *** Roy e-08 *** 419
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