Heterosis and Combining Ability Analysis Oil Content Seed Yield and its Component in Linseed

Transcription

1 Internatonal Journal of Current Mcrobology and Appled Scences ISSN: Volume 6 Number 11 (017) pp Journal homepage: Orgnal Research Artcle Heteross and Combnng Ablty Analyss Ol Content Seed Yeld and ts Component n Lnseed Shalendra Kumar*, P.K. Sngh, S.D. Dubey, S.K. Sngh and Alankar Lamba Department of Genetcs and Plant Breedng, Chandra Shekhar Azad Unversty of Agrculture and Technology, Kanpur-08 00, Inda *Correspondng author A B S T R A C T K e y w o r d s Dalle, Combnng ablty, Heteross and Lnum ustatssmum L. Artcle Info Accepted: 1 September 017 Avalable Onlne: 10 November 017 Ths study was undertaken to estmate the combnng ablty n lnseed through dallel analyss nvolvng eght dverse genotypes. A 8 x 8 full dallel crosses study, ncludng the recprocals, wth lnseed (Lnum ustatssmum L.) was performed to determne both the magntude of gene acton and heterotc performance of the crosses for seed yeld, ol content and mportant yeld components. Feld experments were conducted at the nvestgaton research farm, Nawabganj, C. S. Azad Unversty of agrculture and technology Kanpur. All 56 F1 and F hybrds and ther parents were sown n a randomzed complete block desgn wth 3 replcates. Addtve genetc varance s the result of addtve gene acton whereas non addtve varance s made up of domnance and epstass gene acton. The mean squares of the general combnng ablty (GCA), specfc combnng ablty (SCA) and recprocal combnng ablty (RCA) were statstcally sgnfcant for all trats evaluated. The parents RKY-19, OLC-60, PADMINI, TL-7, SJKO-60, T L-11, S- 36 and KL-31were good general combner for almost the characterstcs evaluated. The sgnfcant postve batter-parent heteross values were obtaned wth several crosses n mportant yeld components. In concluson, the parents used n ths study exhbted postve GCA effects for seed yeld. Therefore they could be consdered as promsng parents n the producton of F1 hybrds and n further breedng studes. Introducton Lnseed (Lnum ustatssmum L.) s a dplod (n =30, genome sze ~370 Mb) selfpollnated annual olseed plant. It has been under the cultvaton for ts seed or stem fbre (Flax) of both (dual purpose) for 1000 years (Dllman, 1953). Every part of the lnseed plant s utlzed commercally ether drectly or after processng. On a very small scale, the seed s drectly used for edble purposes and about 0 % of the total ol produced s used n farmer home. About 80% of the ol goes to the ndustres for the manufacturng of rapdly 1504 dryng pants, varnsh, ol cloths, polymer lnoleum, pad-nk, prntng nk, etc. The ol cake s a good feed for mlch cattle. The ol contans dfferent fatty s lke alpha lnolenc (omega-3) 53.1%, lnolec (omega-6) 17%, olec 18.51%, stearc 4.4% and palmtc/palmtolec 4-6%. Lnseed s the rchest source of omega-3 fatty and t contans almost twce as much as of omega-3 n fsh ol. The rato of omega-3: omega-6 present n lnseed s about 4:1, so ths s a best herbal source of omega-3

2 for mprovement n human metabolsm (Vorca-MrelaPopa, 01). Through dallel analyss a number of parental lnes can be tested n all possble combnatons. Thus, the man objectve of the present study was to dentfy the best combners and ther crosses on the bass of ther general and specfc combnng ablty for ol content and ts qualty parameters. Hybrd s an alternatve approach to ncrease the productvty and most mportant step n the hybrd breedng program s the detecton of sutable parents wth hgh general (gca) and specfc combnng ablty (sca) for gran yeld and then the explotaton of heteross. The study of heteross has a drect bearng on the breedng methodology to be employed for varetal mprovement and also provdes useful nformaton about usefulness of the parents n breedng programs. Materals and Methods Expermental materal and desgn The materal for the nvestgaton comprsed of eght mproved strans/varetes of lnseed namely RKY-19, OLC-60, PADMINI, TL-7, SJKO-60, T L-11, S- 36, KL-31 havng desre genetc varablty for ol content, yeld and assocated attrbute. Parental seed were collected from Project Coordnatng Unt (Lnseed) C. S. Azad Unversty. All possble crosses were made durng rab n a complete dallel fashon (8 8). The F1 and F along wth ther parents were grown n randomzaton block desgn usng three replcaton durng rab season at the nvestgaton research farm, Nawabganj, C. S. azad Unversty of agrculture and technology Kanpur. Analyss of varance The analyss of varance for the expermental desgn was based on the model P jk = µ + v j + b k = e jk (, j = 1...,t; k = 1...b) Where P jk = the phenotype of jk th observaton µ = the populaton mean v j = the progeny effect b k = the block effect e jk = the error term for jk th observaton On the bass of above model, the data obtaned were frst subjected to randomzed block analyss. The skeleton of analyss of varance s gven as under Combnng ablty analyss Combnng ablty analyss was performed accordng to the procedure suggested by Grffng (1956b) Method 1, Model I. In ths model parents, drect crosses and recprocals crosses are ncluded for the analyss. Ths method permts estmaton of recprocal dfferences. It s also assumed that error s ndependently and normally dstrbuted wth the mean zero and error varance e. The analyss of varance for combnng ablty was based on the followng mathematcal model: X jk gˆ gˆ j sˆ (,j = 1,..., n; 1 = 1,,... b) Where = the populaton mean ĝ = the general combnng ablty (gca) for th parent ĝ j = the gca of the j th parent j b k e jk 1505

3 ŝ j = the specfc combnng ablty (sca) for the cross between the th and j th parents such that s j = s j b k = block effect e jk = the envronmental effect assocated wth the jkl th ndvdual observaton on th ndvdual n k th block wth th as female parent and j th as male parent. b = number of blocks/replcatons The restrctons mposed on ths model are: g = 0 and g j j s 0 (For each ), where = varety Where, b = number of replcatons c = number of progenes (parents + F 1 s) r = number of recprocals ( x. x n S g = M'e = Me/bc Where, 1 b = number of replcatons ) X ( n 1( n ) c = number of observatons per plot.. M e = the error m.s.s. obtaned from prevous ANOVA S g = the sum of squares (s.s.) due to gca S s = the sum of squares (s.s) due to sca n = numbero f parents x. = total of array nvolvng th as female x = the value of the th parent of the array x..= the grand total x j = the value of the cross wth th as female and j th as male parents. Estmates of varous effects General Combnng Ablty Effects (GCA) g = (1/)(X. + X.) X../n Where: g = General combnng ablty effects for lne F1 s. n = Number of parents/varetes X. = Total of mean values of F 1 s resultng from crossng jth lnes wth th lnes. X. = Total of mean values of F 1 s resultng from crossng the th lne wth the jth lne. X = Grand mean of all the mean values n the table Specfc Combnng Ablty Effects (SCA) s j = (1/)(X j + X j ) (1/)(X.+X. +X j.+x. j ) + X../ n Where: s j = Specfc combnng ablty between th and jth lnes. X j = Mean value of the F 1 resultng from crossng the th and jth lnes. X j = Mean values for F 1 resultng from crossng the jth and th lnes. 1506

4 X. = Total of means of F 1 s resultng from crossng jth lne wth th lne. X. = Recprocal values of Y. X j. = Total valves for F 1 s resultng from crossng the th lne wth jth lne. X.j = Values of recprocal F 1 s of Y. j. X. = Grand values of the observatons. Recprocal Effects (REC) r j = (X j X j )/ Where: r j = Recprocal effects of the th and jth lnes. X j = Mean values for the F 1 resultng from crossng the th and jth lnes. X j = Recprocal effects of F 1 resultng from Xj. Estmated varances of the estmates of the effect and ther dfferences: Est. Var. gˆ n 1 ˆ e n ( n n ) Est. Var. ŝ, where j j ( n 1)( e n ) Est. Var. ĝ ĝ, where j j e n Est. Var. n ˆ ŝ ŝ ˆ, where j j k n Estmaton of heteross The magntude of heteross was calculated wth the help of the formulae gven below: e ˆ Heteross over better parent (%) = F 1 B P B P Where, x 100 BP = the value of the better parent. Analyss of varance The analyss of varance for combnng ablty (Table 1) revealed hghly sgnfcant varance for both general and specfc combnng ablty n both generatons for all the characters, ndcatng the mportance of both addtve and non-addtve gene acton n the expresson of these trats. Recprocal effects of maternal and paternal combnng ablty showed that use n both form of parent for almost characters. However, addtve and non-addtve effects were predomnant for all the characters, as reported by varous workers Sngh et al., (008), Brahm Sngh et al., (008), Sngh et al., (009), Pal and Mehta (014), Addtve genetc varance s the result of addtve gene acton whereas non addtve varance s made up of domnance and epstass gene acton. The domnance varance declne by half wth each other generaton of selfng or n proportonal reducton of heterozygosty, so t s un-explotable n pure lne. The epstatc varance s also reduce on selfng but ts addtve x addtve reman constant, whch s fxable. The estmate of σ g and σ s and ther rato σ g/σ s ndcated a predomnant role of addtve gene acton and non-addtve gene acton n F 1 and F generaton respectvely. The dfferent estmate obtaned I F 1 and F generaton grow n the same envronment may be attrbute to the restrcted samplng n the total varablty avalable n F or may be due to lnkage. Robnson et al., (1960) reported that f there 1507

5 was preponderance of repulson phase of lnkage, addtve genetc varance could ncrease (.e. non-addtve to addtve) as the generaton were advance and f the lnkage phase was predomnantly couplng, addtve genetc varance could decrease (.e. addtve to non-addtve). The estmated value of σg were hgher than those of σg, σr ndcatng the predomnance of addtve gene acton for days to 50% flowerng, n F1 generaton; plant heght n F generaton. Whch ndcated the predomnance of addtve gene acton for these characters. Sngh et.al. (004). The value of σ sca and σ rca were hgher than those of σg, ndcatng the predomnance of non-addtve gene acton for number of prmary branch, capsule sze, day to maturty, number of seed per capsule, 1000 seed weght, ol content, all fatty s n both generaton; seed yeld per plant n F generaton. The rato σg/ σs was observed more than unty or closer to unty for days to 50 % flowerng n F 1 and plant heght and number of prmary branch n F generaton whch showed preponderance of addtve gene acton whle rest trats showed preponderance of non-addtve gene acton. Combnng ablty General combnng ablty The nformaton regardng gca effect of parents s of prme mportance as s help n successful predcton of genetc potentalty of crosses whch produce desrable ndvduals n segregatng generaton as the choce of parents for hybrdzaton s normally based on per se performance. The gca effect of parents was dentfed as good general combner for all the characters n both generaton. Parent KL-13 was found good general combner for characters stearc, olec and lnolec ; OLC-60 was found good general combner for characters plant heght, days to 50% flowerng, ol content, palmtc and stearc ; Padmn was found good general combner for characters plant heght, days to 50% flowerng, number of capsule per plant, capsule sze, days to maturty, 1000 seed weght, seed yeld per plant, ol content and olec ; RKY-19 was found good general combner for characters plant heght, days to 50% flowerng, leaf area, days to maturty and lnolec ; S-36 was found good general combner for characters stearc and lnolec ; SJKO-50 was found good general combner for characters days to maturty and 1000 seed weght; TL-11 was found good general combner for number of capsules per plant and lnolenc ; TL-7 was found good general combner for leaf area, ol content and lnolenc. It ndcated that per se performance of parents would provde an ndcaton of ther general combnng ablty for the utlzaton of them n hybrdzaton programme. The analyss of varance table for Method 1, Model I (parents and one set of F 1 s and ts recprocal) wth expectatons of mean sum of square s as follows Source d f S.S. M.S.S. Expectatons of M.S.S. 'F' test Gca (n-1) S g M g e+n/(n-1) g M g /M e for n-1, (b- 1)(c-1)(r-1)d f Sca n(n-1)/ S s M s e+/n(n-1)) s j M s /M e for n(n-1)/, (b-1)(c-1) (r-1)d f recprocals n(n-1)/ S r M r e+/n(n-1)) r j M r /M e for n(n-1)/, (b-1)(c-1)(r-1)d f Error (b-1)(c-1) (r-1) S e M' e e 1508

6 Source of varaton Table.1 (a) Analyss of varance for combnng ablty n 8 parent dallel cross (parents and ther F 1 s) among 16 th characters n Lnseed d..f. Plant heght (cm) Day to50%flo werng leaf area No. of prmary branch No.of capsules per plant Capsule sze(mm) Days to maturt y No. of seed per capsule GCA ** ** 0.08** 0.63** ** 0.34** 39.97** 1.3** SCA ** 5.41** 0.0** 0.34** ** 0.06** 14.34** 0.55** recprocal ** 11.83** 0.55* 0.3** 7.74** 0.09** 6.71** 0.60** Error σ g σ s σ recprocal (σ g/ σ s) Source of varaton d..f seed weght Seed yeld per plant Ol content % Palmtc Stearc Olec Lnolec Lenolenc GCA 7.45** 4.5** 19.67** 6.0** 15.91** 9.94** 31.7** 10.83** SCA ** 1.18** 4.74** 15.49** 19.58** 9.19**.0** 40.3** recprocal ** 0.59** 3.06** 13.36** 9.34**.8** 1.03**.41** Error σ g σ s σ recprocal (σ g/ σ s) Note: * sgnfcant at p=0.05 and ** sgnfcant at p=

7 Source of varaton Table.1 (b) Analyss of varance for combnng ablty n 8 parent dallel cross (parents and ther F s) among 16 th characters n Lnseed d..f. Plant heght (cm) Day to50%flo werng leaf area No. of prmary branch No.of capsules per plant Capsule sze(mm) Days to maturt y GCA ** 44.6** 0.04** 0.37* 38.89** 0.40** 77.48** 0.57* SCA 8 9.4** 6.0** 0.03** ** 0.18** 8.3** 1.08** recprocal ** 6.77** 0.06** 0.34** 3.55** 0.1** 10.89** 1.16** Error σ g σ s σ recprocal (σ g/ σ s) Source of varaton d..f seed weght Seed yeld per plant Ol content % Palmtc Stearc Olec Lnolec No. of seed / capsule Lenolenc GCA 7.04**.84** 9.38** 1.41** 13.39** 17.9** 31.6** 10.6** SCA ** 0.57** 8.77** 14.10** 6.57** 1.7** 16.5** 38.04** recprocal ** 0.87** 8.63** 1.17** 11.76** 10.59** 7.99** 50.14** Error σ g σ s σ recprocal (σ g/ σ s) Note: * sgnfcant at p=0.05 and ** sgnfcant at p=

8 Table. Estmates of mean performance and gca effect of 8 dallel parents for 16 th characters n Lnseed Parents Plant heght (cm) Day to50% flowerng leaf area Number of prmary branch GCA effect Mean GCA effect Mean GCA effect Mea GCA effect Mea F 1 F F 1 F F 1 F n F 1 F n KL ** 3.93** **.38** ** ** OLC ** -1.33** ** -1.1** ** PADMINI -9.10** -7.5** ** -.14** * -0.06** * -0.0* 5.33 RKY ** -3.49** ** -.0** * 0.09** S ** ** 0.60** * ** SJKO ** 0.88** ** 1.9** ** TL-11.80** 3.17** ** 1.04** ** * 6.33 TL-7.30**.16** ** ** 0.05** ** -0.4** 5.00 SE±(G) SE±(G-Gj) Table. Contnued Parents Number of capsules per plant Capsule sze(mm) Days to maturty No. of seed per capsule GCA effect Mean GCA effect Mean GCA effect Mean GCA effect Mea F 1 F F 1 F F 1 F F 1 F n KL ** -4.87** ** -0.3** **.67** OLC * 1.00* ** -0.09* ** PADMIN 11.48** 10.54** ** 0.9** ** -1.41** ** I RKY ** -1.35** * ** -.6** ** -0.8* 9.00 S ** -1.91** * ** ** SJKO ** -4.0** ** ** -1.89** TL **.5** **.85** TL-7-3.8** -1.89** **.7** ** SE±(G) SE±(G- Gj)

9 Table. Contnued Parents 1000 seed weght Seed yeld per plant Ol content % Palmtc GCA effect Mean GCA effect Mean GCA effect Mean GCA effect Mea F 1 F F 1 F F 1 F F 1 F n KL ** -0.34** ** -0.38** ** -1.4** ** -0.38** 6.7 OLC * ** ** 0.39** ** 0.43** PADMIN 0.5** 0.45** ** 0.88** **.69** ** 0.3 8/.55 I RKY ** -0.50** ** -0.9** ** -0.69** * 0.43** 8.9 S * ** -0.08** * -1.44** * SJKO * 0.51** ** ** TL ** ** ** -0.67** ** TL * ** -0.14** ** 0.94** * -1.81** 6.38 SE±(G) SE±(G- Gj) Table. Contnued Parents Stearc Olec Lnolec Lnolenc GCA effect Mean GCA effect Mean GCA effect Mea GCA effect Mea F 1 F F 1 F F 1 F n F 1 F n KL ** 0.9* ** 0.69** ** 1.11** ** -1.74** 5.49 OLC ** 0.53** ** ** -0.46** ** -0.41* PADMINI -0.44** -0.45** ** 1.** ** -0.93** RKY ** ** ** 1.08** ** -.15** S ** 0.87** ** -0.53** ** 0.40** ** -0.77** SJKO ** 0.95** ** **.11** ** -.98** TL ** ** -.1** ** -1.39** ** 3.00** TL ** -1.78** ** 0.43** ** -1.9** ** 5.1** SE±(G) SE±(G-Gj) Note: * sgnfcant at p=0.05 and ** sgnfcant at p=

10 Table.3 Heteross range, number of desrable hybrds and best hybrds (better parental) for 16 trats n lnseed Trats Heteross type Heteross range Number of desred hybrds Best hybrds Plant heght (cm) BP to KL-13 x Padmn Days to 50 % flowerng BP to S-36 x TL-11 Leaf area BP to KL-13 x OLC-60 Number of prmary BP to TL-7 x RKY-19 branches per plant Number of capsules per BP to TL-7 x RKY-19 plant Capsule sze BP to OLC-60 x KL-13 Days to maturty BP -1.4 to TL-11 x KL-13 Number of seeds per BP to Padmn x S-36 capsule 1000 gran weght BP to KL-13 x OLC-60 Seed yeld per plant (g) BP to RKY-19 x SJKO-50 Ol content (%) BP to SJKO-50 x OLC-60 Palmtc BP to TL-7 x SJKO-50 Stearc BP to KL-13 x TL-7 Olec BP to RKY-19 x S-36 Lnolec BP to Padmn x OLC-60 Lenolenc BP -8.4 to RKY-19 x TL

11 Combnng ablty descrbes the breedng value of parental lnes to produce hybrds (Romanus et al., 008). The general combnng ablty has been equated wth addtve gene acton and specfc combnng ablty wth non-addtve gene acton (Grffng 1956 a). The analyss of varance for combnng ablty was done for all the 16 characters (Table ). Hghly sgnfcant varances, of general, specfc and recprocal combnng ablty, were observed whch ndcated the mportance of both addtve and non-addtve gene effects for all the n trats both generatons. Specfc combnng ablty effects In general, sca effects do not make any worthwhle contrbutons n the mprovement of self-fertlzng crops expect where there s possblty of commercal explotaton of heteross. Breeders nterest normally, however, rests n obtanng transgressve segregants through crosses n order to produce homozygous lnes n autogamous crops lke lnseed. Jnks and Jones (1958) further emphaszed that superor per se performance of the hybrds mght ndcate ther ablty to produce transgressve hybrds may not ndcate ther ablty to between heteross and non-segregants due to close correspondence between heteross and nonaddtve gene effects. Therefore, study of sca and rca n segregatng generatng generaton would be a better preposton for heteross breedng. Padmn SJKO-50 for plant heght; KL-13 OLC-60, KL-13 RKY- 19, S-36 SJKO-50 for leaf area; KL-13 SJKO-50, KL-13 S-36, S-36 TL-11 number of prmary branch per plant; RKY-19 TL-11, Padmn TL-7, KL-13 Padmn for number of capsules per plant; S- 36 TL-7 for capsule sze; Padmn RKY- 19 for day to maturty; OLC-60 SJKO-50, RKY-19 TL-11 number of seed per sapsule; OLC-60 SJKO-50, Padmn RKY-19, RKY-19 SJKO-50, TL-11 TL-7 and KL- 13 S-36 for 1000 seed weght; Padmn RKY-19, OLC-60 SJKO-50, Padmn TL- 7, KL-13 Padmn for seed yeld per plant; Padmn S-36, KL-13 Padmn for ol content; Padmn TL-7, SJKO-50 TL- 11, Padmn S-36 for palmtc ; OLC-60 Padmn, OLC-60 S-36, SJKO-50 TL- 11 for stearc ; Padmn TL-11 for olec ; RKY-19 S-36, KL-13 S-36 for lnolec and RKY-19 TL-7, RKY-19 TL-11, Padmn TL-11 for lnolenc were found good specfc combner as well as per se performance n F 1 populaton. Padmn SJKO-50 for plant heght; Padmn TL- 11, Padmn TL-7 for days to 50% flowerng; SJKO-50 TL-7 for leaf area; KL-13 S-36 for number of prmary branch per plant; OLC-60 TL-11, KL-13 Padmn, Padmn TL-7 number of capsules per plant; S-36 TL-7, Padmn SJKO-50 for capsule sze; S-36 SJKO-50, RKY-19 SJKO-50 for days to maturty; OLC-60 TL-7, Padmn S-36 number of seed per capsule; Padmn S-36, OLC-60 TL-7, RKY-19 SJKO-50 for seed yeld per plant; RKY-19 S-36, SJKO-50 TL-11, Padmn TL-7 for ol content; Padmn RKY-19, OLC-60 TL-11, KL-13 SJKO- 50 for palmtc ; S-36 SJKO-50, KL- 13 SJKO-50 stearc ; OLC-60 S-36, Padmn TL-7, OLC-60 TL-11, RKY-19 TL-7 for olec ; KL-13 Padmn, OLC-60 RKY-19, S-36 SJKO-50 for lnolec and KL-13 TL-7, S-36 TL-7 lnolenc were found good specfc combner as well as per se performance n F populaton. None of the crosses showed sgnfcant rca effects for the characters wth the expresson of plant heght. The sgnfcant rca effects for days to 50% flowerng n S-36 Padmn; leaf area n OLC-60 KL-13; number of seeds per capsule n TL-7 RKY-19, TL-11 RKY-19; ol content Padmn KL-13; 1514

12 palmtc n TL-7 Padmn; stearc n TL-11 SJKO-50. Olec n TL-11 Padmn; lnolec n S-36 KL-13; lnolenc n TL-11 Padmn, TL-7 RKY-19 n F1 generaton. Day to 50% flowerng n TL-11 KL-13; number of prmary branches per plant n S-36 KL-13; number of capsules per plant n Padmn KL-13, TL-11 RKY-19; number of seeds per capsule n S-36 Padmn; 1000 seed weght n RKY-19 x OLC-60; seed yeld per plant n TL-7 OLC-60, SJKO-50 RKY- 19; ol content n S-36 RKY-19, TL-11 SJKO-50; palmtc n TL-11 OLC-60; olec n TL-7 Padmn, TL-11 OLC- 60; lnolec n RKY-19 OLC-60 n F generaton. It s noteworthy that the crosses, showng consstently postve sca effects also exhbtted postve sgnfcant heteross. Thus, the results of the present study ndcated some relatonshp between sca effects and heteross. It s therefore suggested that sca performance may be consdered as a crteron for selectng the best crosses n lnseed. It may also be worthwhle to attempt b-parental matng n the segregatng generaton among selected crosses to permt superor recombnatons. All the mportant crosses nvolvng parents wth hgh average, average /average and average /low general combners, ndcated that non-addtve type of gene actons, whch are unfxable n nature were nvolved n selected cross combnatons. The study demonstrates that both addtve (fxable) and non-addtve (non-fxable) components of genetc varances were nvolved n governng the nhertance of almost all the quanttatve and qualty trats, although addtve genetc varance was predomnant. Therefore, bparental matng and dallel selectve matng whch may allow ntermatng of the selects n dfferent cycles and explot both addtve and non-addtve gene effect could be useful n the genetc mprovement of the characters of lnseed. Incluson of F 1 hybrds showng hgh sca and havng parents wth good gca, nto multple crosses, could also be a sgnfcant approach for tangble mprovement of almost trats. Heteross Heteross breedng plays an mportant role n crop mprovement for obtanng hgher producton. The degree of heteross should preferably be measured by superor n F 1 hybrd over batter parent or best commercal varety. In the present nvestgaton, heteross was over batter parents for all the sxteen characters studed were found wth all crosses. A wde varaton of heteross range, number of desred hybrds and best hybrd was found for most of the trats (Table 3). Sngh et al., (004) stated that the superorty of hybrds partcularly over hgh parent s more useful for commercal explotaton of heteross and also ndcated the parental combnatons capable of producng the hghest level of transgressve segregants. References Dllman, AD., Classfcaton of flax varetes, 1946 US Dept. of Agrculture, 1953.Seres Informaton. Techncal Bulletn/UNnted States Department of Agrculture.no US Dept of Agrculture, Washngton. Grffng B a. Concepts of specfc and general combnng ablty n relaton to dallel crossng systems. Australan Journal of Bologcal Scences 9: Jnks, J.L. and Jones, J.M. (1958). Estmaton of components of heteross. Genetcs. 43: Romanus K G, Hussen S and Mashela W P Combnng ablty analyss and assocaton of yeld and yeld components among selected cowpea lnes. Euphytca 16:

13 Sngh H, Sharma S N and San R S Heteross studes for yeld and ts components n bread wheat over envronments. Heredtas 141: Sngh,-H-C; Dxt,-R-K; Pathak,-R-K; Rajendra-Sngh; Naln-Twar(004) Genetc analyss of qualty trats n lnseed (Lnum ustatssmum L.). Vorca Mrela Popa, Alexandra Grua Dana Raba, Dela Dumbrava, Camela Moldovan, Despna Bordean, Constantn Mateescu (01) Fatty s composton and ol characterstcs of lnseed (Lnum ustatssmum L.) Banat s Unversty of Agrcultural Scences and Veternary Medcne, Tmsoara, Calea Aradulu, 119, Romana. How to cte ths artcle: Shalendra Kumar, P.K. Sngh, S.D. Dubey, S.K. Sngh and Alankar Lamba Heteross and Combnng Ablty Analyss Ol Content Seed Yeld and Its Component n Lnseed. Int.J.Curr.Mcrobol.App.Sc. 6(11): do: