Background Saw new trap on Biogents website Could not find any published research on new trap Ordered 4 Mosquitaire traps from manufacturer in Germany
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1 Testing Novel Mosquito Traps, Fairfax County, VA, 2010 Joshua Smith FairfaxCountyHealth Department
2 Background Saw new trap on Biogents website Could not find any published research on new trap Ordered 4 Mosquitaire traps from manufacturer in Germany Original quote in Euros Did not accept credit cards Tax forms
3 Background II Told of second new trap while waiting for paperwork to clear Cheaper and just as effective (according to manufacturer) Modified order Three Mosquitaire traps Three Mosquitito traps Original inquiry Receipt oftraps: 54 days
4 Methods Study Design Latin Square Trap Rotation Four days/week Four weeks Each trap at each location one day/week Rotate starting point each week
5 Week One Day 1 Day 2 Day 3 Day 4 Site 1 Site 2 Site 3 Site 4 Mosquitito Mosquitaire no CO 2 BGS Mosquitaire with CO 2 Mosquitaire with CO 2 Mosquitito Mosquitaire no CO 2 BGS BGS Mosquitaire with CO 2 Mosquitito Mosquitaire no CO 2 BGS Mosquitaire with CO Mosquitaire no CO 2 Mosquitito
6 Trap Locations ft 50 m Each morning, traps were rotated through the four trapping tapp gstato stations. s
7 Methods II Tarps placed above each trap site 12 V battery 24 hour trap period August September m apart and 2 10m in the woods
8 BG Sentinel CO 2 BG Lure Color contrast Convection currents Collects day andnight night feeding host seeking seeking mosquitoes
9 Mosquitaire [t]he most efficient trap against the Asian Tiger mosquito Sweetscent Visual cues Air currents Consumer version of BG Sentinel Does not require carbon dioxide Tested with and without CO 2
10 Mosquitito Developed for Asian Tiger mosquito Sweetscent Visual cues Air currents Sweetscent /Sweetskin Lure
11 Methods III Statistical Methods Log (x+1) transformation One way ANOVA Post Hoc Tukey s b, Games Howell
12 Results
13 Female Mosquitoes Collected BGS MA CO 2 MA NO MTT Total CO 2 Total Ave/trap
14 Species Diversity Tx. rutilus Ps. howardii Ps. ferox Ps. columbiae Oc. trivitattus Oc. triseriatuss Oc. japonicus Cx. salinariuss Cx. erraticus Cx. spp. Cq. perturbans An. quadrimaculatus An. punctipennis Ae. vexans Ae. albopictus BG X X X X 0 X 0 0 X X X 0 X 0 X MA CO 2 X X X X 0 X X 0 X X 0 0 X 0 0 MA NO X X 0 0 X X 0 X MTT X X X 0 X X X X X X X 0 X X X BG: 11 MA CO 2 : 10 MA NO: 5 MTT: 15
15 Species Diversity Tx. rutilus Ps. howardii Ps. ferox Ps. columbiae Oc. trivitattus Oc. triseriatuss Oc. japonicus Cx. salinariuss Cx. erraticus Cx. spp. Cq. perturbans An. quadrimaculatus An. punctipennis Ae. vexans Ae. albopictus BG X X X X 0 X 0 0 X X X 0 X 0 X MA CO 2 X X X X 0 X X 0 X X 0 0 X 0 0 MA NO X X 0 0 X X 0 X MTT X X X 0 X X X X X X X 0 X X X BG: 11 MA CO 2 : 10 MA NO: 5 MTT: 15
16 Results Ae. albopictus Cx. spp. Oc. japonicus Oc. triseriatus Total BG 337 (21.1) 1) 18 (1.1) 1) 3 (0.2) 19 (1.2) 377 (23.6) MA CO (23.7) 379 (23.7) 41 (2.6) 4 (0.3) 30 (1.9) 454 (28.4) MA NO 114 (7.1) 5 (0.3) 2 (0.1) 1 (0.1) 122 (7.6) MTT 367 (22.9) 61 (3.8) 9 (0.6) 49 (3.1) 486 (30.4) Total 1197 (18.7) 125 (2.0) 18 (0.28) 99 (1.6) 1439 (22.5)
17 Results Ae. albopictus Cx. spp. Oc. japonicus Oc. triseriatus Total BG 337 (21.1) 1) 18 (1.1) 1) 3 (0.2) 19 (1.2) 377 (23.6) MA CO (23.7) 379 (23.7) 41 (2.6) 4 (0.3) 30 (1.9) 454 (28.4) MA NO 114 (7.1) 5 (0.3) 2 (0.1) 1 (0.1) 122 (7.6) MTT 367 (22.9) 61 (3.8) 9 (0.6) 49 (3.1) 486 (30.4) Total 1197 (18.7) 125 (2.0) 18 (0.28) 99 (1.6) 1439 (22.5)
18 Average Oc. triseriatus by Trap Type 7 OtMTT 6 OtMANO OtMACO OtBG week32 week33 week34 week35
19 Oc. triseriatus ti i t Results Significant variation seen between trap types during weeks 33* and 35 Week 33: Mosquitito significantly more than Mosquitaire (no CO 2 ) (Games Howell: p <0.05) Week 35: Mosquitito significantly more than Mosquitaire (no CO 2 ) (Tukey s b: p <0.05) *Variation not homogenous during weeks 32 and 33
20 Average Cx.. spp. by Trap Type 7 CxMTT CxMANO CxMACO 6 CxBG Week32 Week33 Week34 Week35
21 Cx. spp Results Significant variation seen between trap types during weeks 32, 33, and 35 Week 32: Mosquitito significantly more than all other traps (Tukey s b: p <0.05) Week 33: Mosquitito and Mosquitaire (with CO 2 ) significantly more than Mosquitaire (no CO 2 ) (Tukey s b: p <0.05) Week 35: Mosquitito significantly more than Mosquitaire (no CO 2 ) (Tukey s b: p <0.05)
22 Average Ae. albopictus by Trap Type AaMTT AaMANO AaMACO AaBG Week32 Week33 Week34 Week35
23 Ae. albopictus Results Significant variation seen between trap types during weeks 32, 34, and 35 Week 32: Mosquitito, Mosquitaire (with CO 2 ), and BG Sentinel significantly more than Mosquitaire (noco 2 ) (Tukey s b: p <0.05) Week 34: Mosquitito, Mosquitaire (with CO 2 ), and BG Sentinel significantly more than Mosquitaire (no CO 2 ) (Tukey s b: p <0.05) Week 35: Mosquitito, Mosquitaire (with CO 2 ), and BG Sentinel significantly more than Mosquitaire (no CO 2 ) (Tukey s b: p <0.05)
24 Conclusions Both new traps are comparable to BG Sentinel: Ae. albopictus Importance of CO 2 Mosquitito? More repetitions for more robust dataset dt t Other species
25 Interesting Find August 25, 2010 Mosquitaire with CO 2 Culex pipiens Bipolar gynandromorph Expressing both male features and female features on opposite ends of body
26 Acknowledgements DCIP staff Dr. Bruce Harrison
27 Thank you Questions?
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