Improved Peak Shape Performance and Consistency for Analysis of Challenging Polar Compounds
|
|
|
- Shanon Heath
- 5 years ago
- Views:
Transcription
1 Improved Peak Shape Performance and Consistency for Analysis of Challenging Polar Compounds Laura Provoost GC Columns Product Manager Agilent CrossLab Group
2 Challenges when analyzing active polar compounds Variable column to column peakshape performance Limited column lifetime Rerunning samples due to questionable results Instrument downtime for GC system troubleshooting Increased cost of lab operations For active and complex polar compounds adsorption caused by GC flow path activity can be easily avoided ISCC 0, Riva del Garda
3 Agilent Inert Flow Path Solution Ultra Inert Gold Seal Ultra Inert Liner Deactivated Inlet Weldments, Shell and Transfer Lines Deactivated FPD, TCD, NPD/FID Jets Deactivated Capillary Flow Technology Devices Inert MS Source Flexible Metal Ferrules Ultra Inert GC Column and Ultimate Plus fused silica tubing Gas Clean Filter ISCC 0, Riva del Garda
4 A new WAX GC column for analysis of challenging polar compounds: DB-WAX Ultra Inert Part of the Agilent J&W Ultra Inert GC column family Pushes industry standards for consistent column inertness Every Ultra Inert GC column is rigorously tested to ensure optimal, active analyte delivery to the GC or MS detector Test probes have low molecular weights, low boiling points and no steric shielding of their active groups DB-ms UI HP-ms UI Agilent J&W Ultra Inert GC Columns DB-ms UI HP-ms UI Non/low Polar DB-ms UI DB- UI Increasing Polarity Mid Polar Application specific Ultra Inert columns: DB-UI 0D: Environmental semi volatiles DB-Select UI for <>: Pharmaceutical residual solvents New! DB-WAX UI High Polar The result: lower detection limits and more accurate identification of challenging polar analytes ISCC 0, Riva del Garda
5 DB-WAX Ultra Inert Same selectivity as standard DB- WAX Easy upgrade to Ultra Inert performance Improved peak shape performance for polar compounds Inertness performance withstands repeated temperature cycles Improved column-to-column inertness reproducibility Production quality control Analysis of feed stocks and raw materials Support for production operations troubleshooting Method development R&D Applications Food, Flavor and Fragrance labs - Essential and flavor oils - Personal care products - Fragrance standards/allergens - Free fatty Acids, Alcohols, Glycols - Beverages and aroma analysis - Solvents Industrial chemical labs - Solvent purity alcohols, ketones, aldehydes etc - Mixed solvent/chemical formulations (cleaners, paint additives etc.) Pharmaceutical labs Polyethylene glycol structure - Application: USP regulated Residual Solvents confirmation column ISCC 0, Riva del Garda
6 Proof of Inertness: the DB-WAX Ultra Inert Testing Strong test probe mixture Can highlight deficiencies in column activity, while a weak mixture can mask such deficiencies Like all of Agilent s Ultra Inert GC columns, DB-WAX UI GC columns are tested with the industry s most demanding test probe mixture Based on real-world requirements Includes decanal, propionic acid, ethyl hexanoic acid, and ethyl maltol to ensure inertness performance for these active polar compounds Proven with performance summary sheet shipped with each column ISCC 0, Riva del Garda
7 0 0 DB-WAX UI vs. DB-WAX: Improved inertness pa XOffset : 0 YOffset : GCF_DB-WAX mod DBWaxmix.DATA GCF_DB_WAX UI_USF0_h0C_DB-WAX.DATA 0. Peak DB-WAX UI mixture -Nonanone Decanal Propionic acid Ethylene glycol Heptadecane Aniline DB-WAX UI. Peak DB-WAX UI mixture Methyl dodecanoate -Chlorophenol -Undecanol 0 Nonadecane -Ethylhexanoic acid Ethyl maltol Ethyl maltol DB-WAX.0 RT [min] 0 0 ISCC 0, Riva del Garda
8 Inertness Lifetime at maximum temperature 0 C and 0 C pa XOffset : 0 YOffset : DB-WAX UI after 0h at 0 o C DB-WAX UI after h at 0 o C GCF_DB_WAX UI_USF0_h0C_DB-WAX.DATA GCF_DB_WAX UI_USF0_0h0C_DB-WAX.DATA RT [min] Peak DB-WAX UI mixture -Nonanone Decanal Propionic acid Ethylene glycol Heptadecane Aniline Methyl dodecanoate -Chlorophenol -Undecanol 0 Nonadecane -Ethylhexanoic acid Ethyl maltol ISCC 0, Riva del Garda
9 Durability of Inertness of DB-WAX UI (modified Grob mix) pa XOffset : 0 YOffset : DB-WAX UI after 0h at 0 o C a. a b. DB-WAX UI after h at 0 o C.0 Excellent peak shapes are maintained across all compound classes! b Peak Modified Grob Dicyclohexylamine nc-fame 0,-Dimethylaniline,-Dimethylphenol -Ethylhexanoic acid Ethyl maltol Peak Modified Grob Decane Dodecane Decanal,-Butanediol -Octanol C0 FAME nc-fame GCF_DB_WAX UI_USF0_h0C_Grobmix.DATA GCF_DB_WAX UI_USF0_0h0C_Grobmix.DATA. RT [min] ISCC 0, Riva del Garda
10 DB-WAX UI test mix After conditioning h at 0 C pa XOffset : 0 YOffset : GCB_StabilWAX_serial _DB-WAXre_h 0oC_.DATA GCF_StabilWAX ms_ser 0_DB-WAXre_h 0oC_.DATA GCB_Zbw axplus_00_h 0oC_DB-WAX.DATA GCF_DB_WAX UI_USF0_h0C_DB-WAX.DATA. 0 DB-WAX UI 0. Product X.. Product Yms.0 Product Y Peak DB-WAX UI mixture -Nonanone Decanal Propionic acid Ethylene glycol Heptadecane Aniline Methyl dodecanoate -Chlorophenol -Undecanol 0 Nonadecane -Ethylhexanoic acid Ethyl maltol. RT [min] 0 ISCC 0, Riva del Garda
11 DB-WAX UI Test mix after 0 h at 0 C, 0 C = 00 analyses with min 0 C pa XOffset : 0 YOffset : GCB_StabilWAX_serial _DB-WAX_0h 0oC.DATA GCF_StabilWAX ms_ser 0_lf_DB WAX_0h 0oC.DATA GCB_Zbw axplus_00_0h 0oC_DB-WAXre.DATA GCF_DB_WAX UI_USF0_0h0C_DB-WAX.DATA 0. DB-WAX UI. Peak DB-WAX UI mixture -Nonanone Decanal Propionic acid Ethylene glycol Heptadecane Aniline Methyl dodecanoate -Chlorophenol -Undecanol 0 Nonadecane -Ethylhexanoic acid Ethyl maltol Product X.0.. Product Yms Product Y. RT [min] 0 0 ISCC 0, Riva del Garda
12 Analysis of Organic Acids Excellent Peakshape with DB-WAX Ultra Inert No need to run a separate FFAP column for organic acids Standardize on DB- WAX UI to broaden application range Compound I.D. Compound. Methane I.D... Methane Acetone (solvent).. Acetone Acetic (solvent) Acid.. Acetic Propionoic Acid Acid.. Propionoic Isobutyric Acid Acid.. Isobutyric Butyric Acid Acid.. Butyric Isovaleric Acid Acid. Isovaleric Acid. Valeric Acid. Valeric Acid. -Methylvaleric Acid. -Methylvaleric Acid 0. Hexanoic Acid 0. Hexanoic Acid. -Methylhexanoic Acid. -Methylhexanoic Acid. -Ethylhexanoic Acid. -Ethylhexanoic Acid. Heptanoic Acid. Heptanoic Acid. Pyruvic Acid. Pyruvic Acid.. Octanoic Octanoic Acid Acid.. Nonanoic Nonanoic Acid Acid.. Decanoic Decanoic Acid Acid 0 0 Standard Wax Column DB-WAX UI ISCC 0, Riva del Garda
13 FAMEs Analysis: DB-WAX / DB-WAX UI Identical Selectivity,,,0,,,,0, DB-WaxUI 0 a b a b 0,, b,, a,, 0,0 a,b 0 a, b DB-Wax * Retention time locked on peak, Methyl Stearate ISCC 0, Riva del Garda
14 Lavender Oil: DB-WAX / DB-WAX UI 0 m x 0. mm x 0. μm Identical Selectivity pa 0 DB-WAX DB-WAX UI min ISCC 0, Riva del Garda
15 Lavender Oil: Enlarged section GC/MS chromatogram 0 Blue: DB-WAX Red: DB-WAX UI Peak Compound Name Caryophyllene Terpinen--ol Lavandulyl acetate β-farnesene Lavandulol 0 α-terpineol 0 Identical column selectivity is crucial for customers who have built extensive compound libraries using DB-WAX ISCC 0, Riva del Garda
16 GC/FID Distilled Spirit Analysis DB-WAX Ultra Inert 0 m x 0. mm x 0. μm pa 0 % aqueous sample 00. Ethyl acetate. Acetal. -Pentanol. Ethyl valerate. Acetic acid. Isoamyl caproate. Furfural min ISCC 0, Riva del Garda
17 DB-WAX UI: Robust for Aqueous Samples Excellent stability after 00 injections 00th inj th inj th inj 00th inj 0th inj th inj st inj 0 0 min ISCC 0, Riva del Garda
18 With DB-WAX UI, we are seeing better reproducibility of total aldehyde content and less tailing because of the improved inertness. No selectivity change compared to the standard DB-WAX is essential for complex citrus oil analysis. In addition, time spent for pre-testing new columns is eliminated. Dr. Andreas Böker MCI Miritz Citrus Research & Development Director QC ISCC 0, Riva del Garda
19 Summary DB-WAX Ultra Inert is excellent choice when analyzing polar compounds DB-WAX Ultra Inert has: Same selectivity as DB-WAX, so it s easy to upgrade to Ultra Inert performance Improved peak shape performance for polar compounds Improved column-to-column inertness reproducibility Inertness performance withstands repeated temperature cycles Excellent robustness for aqueous samples ISCC 0, Riva del Garda
20 Acknowledgements Ngoc A Dang GC Columns R&D Poster E.0 Agilent J&W DB-WAX Ultra Inert GC column, a new PEG GC column with improved inertness reliability and column lifetime Roy Lensen GC Columns R&D Allen Vickers GC Columns Manufacturing Liying Yu GC Columns Manufacturing Yun (Ella) Zou GC Columns Applications Peter Heijnsdijk R&D Project Lead Janice Perez R&D Program Manager Gary Lee GC Columns Product Marketing Manager 0 ISCC 0, Riva del Garda
21 ISCC 0, Riva del Garda
22 For More information: Publication type Title Publication number Technical Note Application Note Application Note Application Note A New PEG GC Column with Improved Inertness reliability and Column Lifetime Analysis of Distilled spirits Using Agilent J&W DB-WAX Ultra Inert Capillary GC Column Analysis of Lavender Essential Oil by Agilent J&W DB-WAX Ultra Inert Capillary GC Column Analysis of Glycols in Toothpaste Using Agilent J&W DB-WAX Ultra Inert Capillary GC Column -EN -EN -EN -EN Brochure Increase Your Analytical Confidence for Polar Compounds -0EN Brochure Flow Path Solutions 0-EN Product Page DB-WAX Ultra Inert ISCC 0, Riva del Garda
23 DB-WAX UI Configurations Part Number Configuration -0UI DB-WAX Ultra Inert 0m x 0.mm x 0.um -0UI DB-WAX Ultra Inert 0m x 0.mm x 0.0um -0UI DB-WAX Ultra Inert m x 0.mm x 0.um -0UI DB-WAX Ultra Inert 0m x 0.mm x 0.um -0UI DB-WAX Ultra Inert 0m x 0.mm x 0.0um -0UI DB-WAX Ultra Inert 0m x 0.mm x 0.um -0UI DB-WAX Ultra Inert 0m x 0.mm x 0.0um -0UI DB-WAX Ultra Inert m x 0.mm x 0.um -0UI DB-WAX Ultra Inert 0m x 0.mm x 0.um -0UI DB-WAX Ultra Inert 0m x 0.mm x 0.0um -0UI DB-WAX Ultra Inert 0m x 0.mm x 0.um -0UI DB-WAX Ultra Inert 0m x 0.mm x 0.0um -0UI DB-WAX Ultra Inert m x 0.mm x.00um -0UI DB-WAX Ultra Inert 0m x 0.mm x 0.um -0UI DB-WAX Ultra Inert 0m x 0.mm x.00um -0UI DB-WAX Ultra Inert 0m x 0.mm x 0.0um -0UI DB-WAX Ultra Inert 0m x 0.mm x.00um -0UI DB-WAX Ultra Inert m x 0.0mm x 0.0um *Custom orderable for diameter cage format for 0 GC ISCC 0, Riva del Garda
24 Improve Lab Productivity and Lower Operational Cost Spend less time on troubleshooting and reruns: With DB-WAX UI GC columns, excellent peak shape and column-to-column inertness reproducibility, as well as retention time stability. Save money on columns and maintenance: DB-WAX UI has extended inertness lifetime that withstands repeated temperature cycling to the upper temperature limits of the column. No need for prequalifying columns: Specific inertness testing during quality control guarantees out-of-the-box inertness performance for every DB-WAX UI column. Implement easily, with minimal workflow interruption: DB-WAX UI GC columns have the same selectivity as standard DB-WAX GC columns. Upgrade to Ultra Inert performance is easy with minimal validation. No need to recreate or modify existing compound libraries that are based on DB-WAX selectivity. Combine with Agilent Inert Flow Path GC Supplies for optimized performance ISCC 0, Riva del Garda
A New PEG GC Column with Improved Inertness Reliability and Column Lifetime Agilent J&W DB-WAX Ultra Inert Polyethylene Glycol Column
A New PEG GC Column with Improved Inertness Reliability and Column Lifetime Agilent J&W DB-WAX Ultra Inert Polyethylene Glycol Column Competitive Comparison Authors Ngoc-A Dang and Allen K. Vickers Agilent
Ultra-Inert chemistry for Trace Level Analysis
Ultra-Inert chemistry for Trace Level Analysis Cikui Liang, Ph.D. Challenges and Needs of Today s Laboratories Challenges Qualification/quantification of trace samples Keep instrument up and running Needs
Industrial Applications with a New Polyethylene Glycol-Based GC Column
Industrial Applications with a New Polyethylene Glycol-Based GC Column Application Note Authors Taylor Hayward, Yujuan Hua, Ronda Gras,, and Jim Luong, Dow Chemical Canada ULC PO BAG 6, Highway, Fort Saskatchewan,
UltiMetal Plus Advanced Chemistry for Stainless Steel Surface Deactivation
UltiMetal Plus Advanced Chemistry for Stainless Steel Surface Deactivation Technical Overview Introduction Inert flow path technology Modern GC and GC/MS instrumentation is an important analytical tool
Water Injections in GC - Does Water Cause Bleed?
Water Injections in GC - Does Water Cause Bleed? Eberhardt Kuhn Applications Chemist April 4, 2001 Practical Advice and Useful Tips for the Analysis of Semivolatile Organics by GC and GC/MS 11:00 a.m.
DiatoSorb-W Diatomaceous Earth: Great Chromatography Millions of Years in the Making
Restek GC DiatoSorb-W Diatomaceous Earth: Great Chromatography Millions of Years in the Making Officially listed USP SA and SNS support material. Ideal for USP and ASTM methods that use white diatomaceous
Evaluation of Capillary Columns for General Performance Parameters
Evaluation of Capillary s for General Performance Parameters Application Authors Mitch Hastings, Eberhardt R. Kuhn and Allen K. Vickers Agilent Technologies, Inc 91 Blue Ravine Road Folsom, CA 95630 USA
DiatoSorb-W Diatomaceous Earth: Great Chromatography Millions of Years in the Making
DiatoSorb-W Diatomaceous Earth: Great Chromatography Millions of Years in the Making Officially listed USP SA and SNS support material Ideal for many USP and ASTM packed column methods Consistent quality
Agilent UltiMetal Plus Stainless Steel Deactivation for Tubing, Connectors, and Fittings
Agilent UltiMetal Plus Stainless Steel Deactivation for Tubing, Connectors, and Fittings Technical Overview Introduction Modern GC and GC/MS instruments are important analytical tools for accurate and
Trajan SGE GC Columns
Trajan Scientific and Medical Trajan SGE GC Columns Trajan Scientific and Medical Our focus is on developing and commercializing technologies that enable analytical systems to be more selective, sensitive
Analyze Hydrocarbon Impurities in 1,3-Butadiene with an Agilent J&W GS-Alumina PT Column
Analyze Hydrocarbon Impurities in,3-butadiene with an Agilent J&W GS-Alua PT Column Application Note Energy and Chemicals Authors Yun Zou and Chunxiao Wang Agilent Technologies Shanghai Ltd Abstract The
Analysis of USP Method <467> Residual Solvents on the Agilent 8890 GC System
Application Note Residual Solvent Analysis of USP Method Residual Solvents on the Agilent 889 GC System Author Lukas Wieder, Jie Pan, and Rebecca Veeneman Agilent Technologies, Inc. 8 Centerville Road
A Direct 5 ms Column Performance Comparison for Active Semi-Volatile Analytes
A Direct 5 ms Column Performance Comparison for Active Semi-Volatile Analytes Application Note Environmental Authors Ken Lynam and Doris Smith Agilent Technologies, Inc. 2850 Centerville Road Wilmington,
Understanding the Capillary GC Column: How to Choose the Correct Type and Dimension
Understanding the Capillary GC Column: How to Choose the Correct Type and Dimension Simon Jones Application Engineer Things to Consider Is it Volatile enough to chromatograph by GC? Is it a Gas or a Liquid?
NEW Agilent J&W Ultra Inert Capillary GC Columns. Proven performance that raises the bar on. consistent. column inertness.
NEW Agilent J&W Ultra Inert Capillary GC Columns Proven performance that raises the bar on consistent column inertness. Finally a column technology that gives you better sensitivity and peak shape for
How To Select the Correct GC Column. Simon Jones Application Engineer
How To Select the Correct GC Column Simon Jones Application Engineer Things to Consider Is it Volatile enough to chromatograph by GC? Is it a Gas or a Liquid? How are we getting the Sample Injected? What
Highly Sensitive and Rugged GC/MS/MS Tool
Highly Sensitive and Rugged GC/MS/MS Tool For Pesticide Multiresidue Analysis in Food Samples Agilent 7 Series Triple Quadrupole GC/MS. The world s first MS/MS designed specifically for GC Analysis Introduction
An Advanced Base Deactivated Capillary Column for analysis of Volatile amines Ammonia and Alcohols.
An Advanced Base Deactivated Capillary Column for analysis of Volatile amines Ammonia and Alcohols. Jaap de Zeeuw, Ron Stricek and Gary Stidsen Restek Corp Bellefonte, USA To analyze basic compounds at
Activity in the FID Detection Port: A Big Problem if Underestimated
Activity in the FID Detection Port: A Big Problem if Underestimated Jaap de Zeeuw, Restek Corporation, Middelburg, The Netherlands It is commonly known in gas chromatography, that many problems can be
Understanding Gas Chromatography
Understanding Gas Chromatography What is Really Going on Inside the Box? Simon Jones GC Applications Engineer Page 1 Group/Presentation Title Month ##, 200X ?? K? Page 2 Typical GC System Gas supply Injector
Headspace Technology for GC and GC/MS: Features, benefits & applications
Headspace Technology for GC and GC/MS: Features, benefits & applications Karima Baudin Oct 2015 Why use Headspace? Very Simple no to minimum sample prep Robust enhance uptime Non-detectable carry-over
Analysis of Sulfur-Containing Flavor Compounds By GC/MS With A PFPD
Analysis of Sulfur-Containing Flavor Compounds By GC/MS With A Application Note 19060203 Keywords Coffee Essential Oil Flavor Fragrance Gas Chromatograph (GC) Sulfur Compounds Abstract Sulfur compounds
BP1 PONA BPX1. GC Capillary Columns BP1 PONA and BPX1
GC Capillary Columns 100% Dimethyl Polysiloxane GC Columns and Applications ID (mm) Film Thickness (µm) Length (m) Temperature Limits ( C) Part No. 0.32 0.5 60-60 to 320/340 054069 0.32 1 60-60 to 320/340
Introduction to Capillary GC
Introduction to Capillary GC LC Columns and Consumables Simon Jones Chromatography Applications Engineer February 20, 2008 Page 1 Introduction to Capillary GC t r K c?? Kβ k = - tr t m? t m R s Page 2
The Suite for Environmental GC Analysis
The Suite for Environmental GC Analysis SGE Environmental GC Columns Performance Selectivity Delivery Promise www.sge.com SGE Environmental GC Columns The Suite for Environmental GC Analysis SGE GC Columns
Virtually Particle-Free Rt -Silica BOND Columns
Petrochemical Applications Virtually Particle-Free Rt -Silica BOND s Provide Reliable PLOT Performance With Less Time Lost for Maintenance By Amanda Rigdon, Bill Bromps, Tom Vezza, and Jaap de Zeeuw Optimized
Secrets of GC Column Dimensions
Secrets of GC Column Dimensions GC Columns and Consumables Simon Jones Application Engineer May 20, 2008 Slide 1 Secrets of GC Column Dimensions Do I have the right column phase? Resolution Equation Changes
Analysis of Food Samples using Thin Film Solid Phase Microextraction (TF-SPME) and Thermal Desorption GC/MS
Analysis of Food Samples using Thin Film Solid Phase Microextraction (TF-SPME) and Thermal Desorption GC/MS Laurel Vernarelli, Jackie Whitecavage, John Stuff GERSTEL, Inc., 701 Digital Drive, Suite J,
Automated Sample Preparation of Headspace Standards Using the Agilent 7696 WorkBench
Automated Sample Preparation of Headspace Standards Using the Agilent 7696 WorkBench Application Note Forensic Toxicology and Drug Testing Author Jared Bushey Agilent Technologies, Inc. 285 Centerville
Product Brief. - Hydrocarbons alkanes, alkenes, alkynes, dienes including natural gas, refinery gas, liquified petroleum gas
Agilent Porous Polymer PLOT Columns: New Products, Expanded Uses, Prices Cut in Half! Product Brief Need improved resolution of small volatile compounds? Didn't try a PLOT column due to high price, short
Column Dimensions. GC Columns and Consumables. Mark Sinnott Application Engineer. March 12, 2010
Secrets of GC Column Dimensions GC Columns and Consumables Mark Sinnott Application Engineer Folsom California March 12, 2010 Page 1 Secrets of GC Column Dimensions Do I have the right column phase? Resolution
Rapid Analysis of Food and Fragrances Using High-Efficiency Capillary GC Columns. Application. Authors. Abstract. Introduction
Rapid Analysis of Food and Fragrances Using High-Efficiency Capillary GC Columns Application Food, Flavors and Fragrances Authors Mark Sinnott and Simon Jones Agilent Technologies, Inc. 9 Blue Raving Road
CHEM340 Tutorial 4: Chromatography
CHEM340 Tutorial 4: Chromatography 1. The data in the table below was obtained from a chromatogram obtained with a 10 cm liquid chromatography column. Under the conditions used, the compound uracil is
Introduction to Capillary GC
?? Kβ? Page 1 Typical GC System Gas supply Injector Detector Data handling GAS Column Oven Page 2 CARRIER GAS Carries the solutes down the column Selection and velocity influences efficiency and retention
Analysis of Trace (mg/kg) Thiophene in Benzene Using Two-Dimensional Gas Chromatography and Flame Ionization Detection Application
Analysis of Trace (mg/kg) Thiophene in Using Two-Dimensional Gas Chromatography and Flame Ionization Detection Application Petrochemical Authors James D. McCurry and Bruce D. Quimby Agilent Technologies
Selection of a Capillary
Selection of a Capillary GC Column - Series 3 Mark Sinnott Application Engineer March 19, 2009 Page 1 Typical Gas Chromatographic System Mol-Sieve Traps Fixed Restrictors Regulators Injection Port Detector
Fast Analysis of Aromatic Solvent with 0.18 mm ID GC column. Application. Authors. Introduction. Abstract. Gas Chromatography
Fast Analysis of Aromatic Solvent with.8 mm ID GC column Application Gas Chromatography Authors Yun Zou Agilent Technologies (Shanghai) Co. Ltd. Ying Lun Road Waigaoqiao Free Trade Zone Shanghai 3 P.R.
Application Note. Gas Chromatography/Mass Spectrometry/Food Safety. Abstract. Authors
Trace-Level Analysis of Melamine in Milk Products on Agilent 789A/5975C GC/MSD Using a ew Agilent J&W DB-5ms Ultra Inert Column and SampliQ SCX Cartridges Application ote Gas Chromatography/Mass Spectrometry/Food
Speakers. Moderator. John V Hinshaw GC Dept. Dean CHROMacademy. Tony Taylor Technical Director CHROMacademy. Dave Walsh Editor In Chief LCGC Magazine
Webcast Notes Type your questions in the Submit Question box, located below the slide window You can enlarge the slide window at any time by clicking on the Enlarge Slides button, located below the presentation
Introduction to Capillary GC. Page 1. Agilent Restricted February 2, 2011
?? Kβ? Page 1 Typical GC System Gas supply Injector Detector Data handling GAS Column Oven Page 2 CARRIER GAS Carries the solutes down the column Selection and velocity influences efficiency and retention
New ZB-5HT Inferno The World s Highest Temperature Non-Metal GC Column
New ZB-5HT Inferno The World s Highest Temperature Non-Metal GC Column The World's First Non-metal 5% Phenyl Phase GC Column Rated to 430 C * Specially processed for thermal stability up to 430 C A true
Selection of a Capillary GC Column
Selection of a Capillary GC Column Mark Sinnott Application Engineer March 13, 2008 Page 1 Typical Gas Chromatographic System Mol-Sieve Traps Fixed Restrictors Regulators Injection Port Detector Electrometer
Practical Faster GC Applications with High-Efficiency GC Columns and Method Translation Software
Practical Faster GC Applications with High-Efficiency GC Columns and Method Translation Software High Efficiency GC Columns Page 1 Variables for Shortening Run Times Stationary Phase Shorten Column Length
Introducing New Functionalities in Liquid Stationary Phases in GC Columns for Confirming Organic Volatile Impurity Testing in Pharmaceutical Products.
Introducing New Functionalities in Liquid Stationary Phases in GC Columns for Confirming Organic Volatile Impurity Testing in Pharmaceutical Products. CHRISTOPHER M. ENGLISH, CHRISTOPHER S. COX, FRANK
Turkish raki is the most popular traditional distilled
YILMAZTEKIN & CABAROGLU: JOURNAL OF AOAC INTERNATIONAL VOL. 94, NO. 2, 2011 611 FOOD COMPOSITION AND ADDITIVES Confirmatory Method for the Determination of Volatile Congeners and Methanol in Turkish Raki
The Importance of Area and Retention Time Precision in Gas Chromatography Technical Note
The Importance of Area and Retention Time Precision in Gas Chromatography Technical Note Abstract Area and retention time are the two primary measurements in gas chromatography. The precision with which
The Analysis of Organic Acids in Foods and Beverages Using Reversed Phase HPLC
The Analysis of rganic Acids in Foods and Beverages Using Reversed Phase HPLC Rebecca E. Wittrig & Terrence S. Reid Restek Corporation Benner Circle, Bellefonte, PA 683 USA Restek Corporation www.restekcorp
Discovering the Scent of Celery: HS-SPME, GC-TOFMS, and Retention Indices for the Characterization of Volatiles
Discovering the Scent of Celery: HS-SPME, GC-TOFMS, and Retention Indices for the Characterization of Volatiles LECO Corporation; Saint Joseph, Michigan USA Key Words: GC-TOFMS, GC-MS, Pegasus BT, HS-SPME,
Introduction to Gas Chromatography
Introduction to Gas Chromatography 31-1 Objectives To know what is chromatography To understand the mechanism of compound separation To know the basic of gas chromatography system 31-2 Chromatography Definition
Pittcon P
PO-CON1701E 76% Increase in Throughput for Determination of Semi-Volatiles Using Narrow-Bore GC Columns and Rapid Data Acquisition with a Highly Sensitive Quadrupole GCMS System Pittcon 2017 1440-14P Brahm
Barry E. Boyes, Ph.D. Consumables and Accessories Business Unit May 10, 2000
Barry E. Boyes, Ph.D. Consumables and Accessories Business Unit May 0, 000 HPLC Column Troubleshooting What Every HPLC User Should Know :00 a.m. EST Telephone Number: 86-650-06 Chair Person: Tim Spaeder
Application Note. Abstract. Authors. Introduction. Food Safety
Ultra Inert (UI) Wool Liner Performance Using an Agilent J&W DB-35ms UI Column with and without an Analyte Protectant for Organophosphorus (OP) Pesticides Application Note Food Safety Authors Ken Lynam
So Many Columns! How Do I Choose? Daron Decker Chromatography Technical Specialist
So Many Columns! How Do I Choose? Daron Decker Chromatography Technical Specialist GC Columns Wall Coated Open Tubulars Liquid phase coated capillaries Internal Diameter 0.05 0.53mm Length 5m 100m Porous
Uses of GC/MS. Synthesis of 1-Bromobutane Butenes from 2-Butanol Fisher Esterification of Benzoic Acid Nitration of Methyl Benzoate
Instrument Uses of GC/MS 0 TechPrep Senior Event 0 Honors Project 0 Gasoline Analysis-General Chemistry 0 Ester Synthesis and Analysis-Chemistry for Liberal Arts Majors 0 Organic Reaction Product Analysis
Jaap de Zeeuw Varian, Inc.. Middelburg, The Netherlands
"Innovations in capillary gas chromatography for improving reproducibility, reliability and ease-of-use in the gas chromatographic separation of complex samples" Jaap de Zeeuw Varian, Inc.. Middelburg,
Hydrogen as a Carrier Gas in the Analysis of Polar/Non-Polar Compounds Using the Polyarc System. Application Note. Author. Introduction.
Hydrogen as a Carrier Gas in the Analysis of Polar/Non-Polar Compounds Using the Polyarc System Application Note Chemicals Author Bob Moyer Sr. Research Scientist, R&D Sasol USA, Performance Chemicals
Chapter 27: Gas Chromatography. Principles Instrumentation Detectors Columns and Stationary Phases Applications
Chapter 27: Gas Chromatography Principles Instrumentation Detectors Columns and Stationary Phases Applications GC-MS Schematic Interface less critical for capillary columns Several types of Mass Specs
GC Resolution Do You See What I See?
GC Resolution Do You See What I See? Column Dimensions and Carrier Gas Optimization Deans Switch Page 1 Variables for Maximizing Resolution Optimized Stationary Phase Longer Column Length Decrease Internal
Agilent J&W GC Column Selection Guide
Agilent J&W GC Column Selection Guide Rely on unsurpassed reproducibility, efficiency, and inertness. Speed your selection with this one-stop resource. Agilent J&W GC Column Selection Guide Table of Contents
LC and LC/MS Column Selection Flow Chart
LC and LC/MS Column Selection Flow Chart To use the column selection diagram below, simply follow the path for your analyte and mobile phase. At the far right, follow your final column selection to the
Chromatographic Methods of Analysis Section: 5 Gas Chromatography (GC) Prof. Tarek A. Fayed
Chromatographic Methods of Analysis Section: 5 Gas Chromatography (GC) Prof. Tarek A. Fayed Gas Chromatography (GC) In gas chromatography, the sample is vaporized and injected onto the head of a chromatographic
Determination of Total Volatile Organic Compounds in Indoor Air Using Agilent 7667A mini TD and 7820A GC
Determination of Total Volatile Organic Compounds in Indoor Air Using Agilent 77A mini TD and 70A GC Application Note Environmental Authors Tingting Bu, Xiaohua Li Agilent Technologies (Shanghai) Co.,
We don t have anything against mass spectrometry. We just think it s time for a worthy alternative.
We don t have anything against mass spectrometry. We just think it s time for a worthy alternative. gas chromatography detector Everything you need in one GC detector Universal and selective detector with
Ultrafast and sensitive analysis of sweeteners, preservatives and flavorants in nonalcoholic beverages using the Agilent 1290 Infinity LC system
Ultrafast and sensitive analysis of sweeteners, preservatives and flavorants in nonalcoholic beverages using the Agilent 129 Infinity LC system Application Note Food and Beverages Author Srividya Kailasam
Introduction and Principles of Gas Chromatography
Introduction and Principles of Gas Chromatography Jaap de Zeeuw Restek, Middelburg, The Netherlands Jaap.dezeeuw@restek.com Definition and Uses of Gas Chromatography GC Components and Types of Columns
Semivolatile Organics Analysis Using an Agilent J&W HP-5ms Ultra Inert Capillary GC Column
Semivolatile Organics Analysis Using an Agilent J&W HP-5ms Ultra Inert Capillary GC Column Application Note Environmental Author Doris Smith and Kenneth Lynam Agilent Technologies, Inc. 285 Centerville
Accurate Analysis of Fuel Ethers and Oxygenates in a Single Injection without Calibration Standards using GC- Polyarc/FID. Application Note.
Accurate Analysis of Fuel Ethers and Oxygenates in a Single Injection without Calibration Standards using GC- Polyarc/FID Application Note Volatile Organic Compounds (VOCs) Author Andrew Jones Activated
Take Your Food Testing to the Next Level
GC Columns Take Your Food Testing to the Next Level Using Zebron GC Columns Featuring Pesticides FAMEs Essential Oils Flavors And more.. www.phenomenex.com/foodgc Pesticide Screening by GC-MS - 7 7 9,9
GC-FTIR Analysis of Lemon Extract: Gram-Schmidt Chromatogram. Solvent Peak(2.20) H 3 C CH Air Peak(2.08)
4.20 Application Note #8 Complex Mixtures for Complex Tastes 0.13 GC-FTIR Analysis of Lemon Extract: Gram-Schmidt The Summary 0.12 0.11 0.10 3 C Solvent Peak(2.20) 3 C The demand for pure and natural flavorings
Analytical Instrumentation
Gas Chromatography and Mass Spectrophotometry Environmental, food safety, toxicology, and forensic gas chromatography applications are more demanding than ever. Our portfolio of GC, GC- MS, GC-MS/MS and
CHEM 429 / 529 Chemical Separation Techniques
CHEM 429 / 529 Chemical Separation Techniques Robert E. Synovec, Professor Department of Chemistry University of Washington Lecture 1 Course Introduction Goal Chromatography and Related Techniques Obtain
Continuous Improvement in Petroleum/Petrochemical Analysis HP s Family of Innovative PLOT Columns
Continuous Improvement in Petroleum/Petrochemical Analysis HP s Family of Innovative PLOT Columns Brochure Brief Porous Layer Open Tubular (PLOT) columns have been replacing traditional packed columns
Quantification of Pesticides in Food without Calibration using GC/FID with the Polyarc Reactor
Quantification of Pesticides in Food without Calibration using GC/FID with the Polyarc Reactor Application Note Pesticides Authors Charlie Spanjers and Paul Dauenhauer University of Minnesota, Twin Cities
Simultaneous dual capillary column headspace GC with flame ionization confirmation and quantification according to USP <467> Application Note
Simultaneous dual capillary column headspace GC with flame ionization confirmation and quantification according to USP Application Note Joseph M. Levy Michael Kraft Abstract Agilent Equipment 7890A
Choosing the Correct GC Column Dimensions and Stationary Phase
Choosing the Correct GC Column Dimensions and Stationary Phase Daron Decker Chromatography Technical Specialist Page 1 Nothing is useless it can always serve as a bad example Custom Column: 150 m x 250
METHOD 8033 ACETONITRILE BY GAS CHROMATOGRAPHY WITH NITROGEN-PHOSPHORUS DETECTION
METHOD 80 ACETONITRILE BY GAS CHROMATOGRAPHY WITH NITROGEN-PHOSPHORUS DETECTION 1.0 SCOPE AND APPLICATION 1.1 Method 80 may be used to determine the concentration of acetonitrile (CAS No. 75-05-8) in aqueous
A Newly Approved ASTM Standard For Analysis of Thiophene in Benzene Using a Pulsed Flame Photometric Detector (PFPD)
Application Note 19010803 A Newly Approved ASTM Standard For Analysis of in Using a Pulsed Flame Photometric Detector (PFPD) Keywords ASTM Standard D4735-96 FPD Flame Photometric Detector Model 5380 PFPD
Identifying Pesticides with Full Scan, SIM, µecd, and FPD from a Single Injection Application
Identifying Pesticides with Full Scan, SIM, µecd, and FPD from a Single Injection Application Food Safety, Environmental Authors Chin-Kai Meng and Bruce Quimby Agilent Technologies, Inc. 2850 Centerville
Column Selection. there is more to life than a boiling point column. Jaap de Zeeuw Restek Corporation, The Netherlands. Copyrights: Restek Corporation
Column Selection there is more to life than a boiling point column Jaap de Zeeuw Restek Corporation, The Netherlands Stationary Phase Selectivity Boiling Point versus Solubility Retention of a compound
GC Instruments. GC Instruments - Sample Introduction
GC Instruments 1 Fairly simple instrumentation Maintaining constant average pressure is important! Pressure controls flow rate T influences retention (k ) Flow rate monitoring Changing flow rate changes
Forensic Toxicology. Analysis of Ethanol in Blood using Master SHS Static Headspace Sampler and Master GC Gas Chromatograph APPLICATION NOTE
Analysis of Ethanol in Blood using Master SHS Static Headspace Sampler and Master GC Gas Chromatograph APPLICATION NOTE Authors: DANI Instruments SpA viale Brianza, 87 Cologno Monzese Milano Italy Key
Principles of Gas- Chromatography (GC)
Principles of Gas- Chromatography (GC) Mohammed N. Sabir January 2017 10-Jan-17 1 GC is a chromatographic technique utilizes gas as the mobile phase which is usually an inert gas (Hydrogen, Helium, Nitrogen
Capillary GC Column Selection and Method Development A Primer on Column Parameters and Instrument Conditions
Capillary GC Column Selection and Method Development A Primer on Column Parameters and Instrument Conditions Michael D. Buchanan September 11, 2014 sigma-aldrich.com/analytical 2012 Sigma-Aldrich Co. All
14.1 Aldehydes and Ketones Copyright 2007 by Pearson Education, Inc. Publishing as Benjamin Cummings
Chapter 14 Aldehydes, Ketones, and Chiral Molecules 14.1 Aldehydes and Ketones Copyright 2007 by Pearson Education, Inc. Publishing as Benjamin Cummings Carbonyl Group in Aldehydes A carbonyl group and
The Low-Temperature Evaporative Light-Scattering Detector (LT-ELSD)
The Low-Temperature Evaporative Light-Scattering Detector (LT-ELSD) Basically all compounds which are less volatile than the mobile phase can be detected. Detection is based on a Universal property of
The end of. mass-speculation. MS Certified Vials Pre-cleaned and certified vials for mass spectrometry
The end of mass-speculation Pre-cleaned and certified vials for mass spectrometry Bonded, ultra-pure closures LC/MS and GC/MS Manufacturing the Ultimate Vial for Mass Spectrometry More than a quarter century
Residual solvents analysis using an Agilent Intuvo 9000 GC system
Residual solvents analysis using an Intuvo GC system Technology advantage: simplified dual column analysis with Flow Chip modularity Introduction USP defines a method for analysis of residual solvents
APPLICATION NOTE. A Capillary Approach to ASTM D3606: Test Method for Determination of Benzene and Toluene in Finished Motor and Aviation Gasoline
A Capillary Approach to ASTM D3606: Test Method for Determination of Benzene and Toluene in Finished Motor and Aviation Gasoline Fast : Cycles in
Application Note 059 Direct Desorption of Car Trim Materials for VOC and SVOC Analysis in Accordance with VDA Method 278
Application Note Direct Desorption of Car Trim Materials for VOC and SVOC Analysis in Accordance with VDA Method Application Note Abstract In this application note, we demonstrate the suitability of Markes
Fast Analysis of USP 467 Residual Solvents using the Agilent 7890A GC and Low Thermal Mass (LTM) System
Fast Analysis of USP 7 Residual Solvents using the Agilent 789A GC and Low Thermal Mass (LTM) System Application Note Pharmaceutical Author Roger L Firor Agilent Technologies, Inc. 8 Centerville Road Wilmington,
GC/MS BATCH NUMBER: T50100
GC/MS BATCH NUMBER: T50100 ESSENTIAL OIL: TURMERIC BOTANICAL NAME: CURCUMA LONGA ORIGIN: INDIA KEY CONSTITUENTS PRESENT IN THIS BATCH OF TURMERIC OIL % Ar-TURMERONE 51.2 -TURMERONE 12.1 -TURMERONE 11.8
Fast and Accurate Analysis of PBDEs in a Single Run, Including
Fast and Accurate Analysis of PBDEs in a Single Run, Including Kory Kelly, Adam Endeman, and Matthew Trass Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 90501 USA PO14700713_2 PO14400613_W_2 Abstract
VGA-100 Instrument Detection Limits and Linearity
VGA-100 Instrument Detection Limits and Linearity The VGA-100 is the world s first benchtop vacuum ultraviolet (VUV) spectrometer for gas chromatography (GC). It is a universal GC detector that provides
Rtx -CLPesticides Columns Reduce Analysis Time; Reduce System Maintenance
Columns Reduce Analysis Time; Reduce System Maintenance 0 YEARS Turning Visions into Reality www.restek.com 00-- --00 and Fused Silica Columns Detect and confirm 0 common organochlorine pesticides simultaneously.
VOC Analysis of Water-Based Coatings by Headspace-Gas Chromatography
VOC Analysis of Water-Based Coatings by Headspace-Gas Chromatography Introduction ASTM International publishes a number of different methods for the determination of volatile compounds in water-based coatings.
The GC-APCI Interface for the Agilent Q-TOF LC/MS System Improves Sensitivity, Mass Accuracy, and Speed for a Wide Range of GC Applications
The GC-APCI Interface for the Agilent Q-TO LC/MS System Improves Sensitivity, Mass Accuracy, and Speed for a Wide Range of GC Applications Technical Overview Authors Paul Goodley Agilent Technologies,
Chemistry Instrumental Analysis Lecture 28. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 28 Two types in general use: -packed (stationary phase) -open tubular or capillary determine selectivity and efficiency of the sample. Column Materials Column
Validation of USEPA Method Using a Stratum PTC and the New AQUATek 100 Autosampler
Validation of USEPA Method 524.2 Using a Stratum PTC and the New AQUATek 100 Autosampler Application Note Abstract Automation is the key to increasing laboratory productivity and minimizing costs. It is
Agilent J&W GC Column Selection Guide SPEED YOUR SELECTION WITH THIS ONE-STOP RESOURCE
Agilent J&W GC Column Selection Guide SPEED YOUR SELECTION WITH THIS ONE-STOP RESOURCE Table of Contents The story behind Agilent J&W Advanced GC Columns... 1 Column Selection Principles... 3 Selecting