BMR Microbiology. Research Article ISOLATION AND IDENTIFICATION OF ENDOPHYTIC FUNGI FROM VARIOUS MEDICINAL PLANTS
|
|
- Clarissa Walton
- 5 years ago
- Views:
Transcription
1 Open Access Scientific Publisher Research Article ISOLATION AND IDENTIFICATION OF ENDOPHYTIC FUNGI FROM VARIOUS MEDICINAL PLANTS ABSTRACT Bhumika Jariwala 1, Binita Desai 1 1 Department of microbiology, Shree Ramkrishna Institute of computer education and Applied Sciences, Athwalines, Surat , India Correspondence should be addressed to Bhumika Jariwala Received February 28, 2018; Accepted March 30, 2018; Published April 07, 2018; Copyright: 2018 Bhumika Jariwala et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Cite This Article: Jariwala, B., Desai. B.(2018). Isolation and identification of endophytic fungi from various medicinal plants. Bmr Microbiology, 4(1). 1-7 The term endophytes includes a family of microorganisms that grow intra-and/or intercellularly in the tissues of higher plants without any symptoms on the plants in which they reside. In the present investigation ten medicinal plants were analyzed for the presence of endophytic fungi. A total of 25 fungal species were isolated from healthy leaves and identified based on the morphology of fungal culture and spores. Among them one unidentified isolate was group to mycelia sterilia fungi. Other twenty-four isolates were identified as 11 species belong to Alternaria spp., Trichophyton spp., Geotrichum spp., Candida spp., Aspergillus spp., Aureobasidium spp., Fusarium spp., Exserohilum spp., Curvularia spp., Coccidioides spp., Bipolaris spp. by morphological characters. KEYWORDS: Endophytic fungi, Identification, Medicinal plants. INTRODUCTION The word endophyte means in the plant (endo-within, phyton-plant) [11]. Plants may serve as a reservoir of large numbers of microorganisms known as endophytes. Endophytes are microorganisms (mostly fungi and bacteria) that inhabit plant hosts for all or part of their life cycle. They colonize the internal plant tissues beneath the epidermal cell layers without causing any apparent harm or symptomatic infection to their host, living within the intercellular spaces of the tissues and its seems that they may penetrate the living cells. Endophytes form inconspicuous infections within tissues of healthy plants for all or nearly all their life cycle and their host tissues appear symptomless, and they remain asymptomatic for many years and only become parasitic when their hosts are stressed [10]. The term Endophyte originally coined by German scientist Heinrich Anton De Bray in 1884 [11]. Endophytic fungus was first identified by Freeman in 1904, and was isolated from Lolium persicum (Persian darnel) [9]. Approximately, there are near to 300,000 plant species on earth and each individual plant is the host to one or more endophytes, and many of them may colonize certain hosts. It has been estimated that there may be as many as one million different endophytic fungal taxa, thus endophytes may be hyperdiverse [10]. Endophytic fungal communities are influenced by many factors such as geographical location, climatic patterns, physiology and specificity of colonized tissue [11]. They are present in most plant parts, especially the leaves, where the tissue is apparently healthy [7]. Endophytes relationship with plant varies from symbiotic to pathogenic. Endophytes benefit host plant by preventing pathogenic organism from colonizing. It has been categorized into two main groups (Clavicipitaceous and non-clavicipitaceous) based on differences in evolution, taxonomy, plants host and ecological functions. Clavicipitaceous are able to infect only some species of grasses and nonclavicipitaceous are found in the asymptomatic tissues of other higher plants. It stimulates plants growth, increase disease resistance, improve the plants ability to withstand environmental stresses and recycle nutrients [14]. 1
2 Isolation of endophytes is a critical step, because it requires sensitivity to recover a maximum number of colonized endophytes and should be accurate enough to eliminate the epiphytic microbes which are present on the plant surface. Endophytes can be isolated from various plant parts such as seeds, leaves and stems. The collected plants for studying endophytic communities should look apparently healthy and disease free plant, i.e. they do not display any visual symptoms of diseases, in order to minimize the presence of plant pathogenic and saprobic species, and to prevent the isolation of localized pathogenic endophytic microorganisms [10]. MATERIAL AND METHODS Plant Collection and Identification Healthy (showing no visual disease) and mature leaves of ten medicinal plants (Table:1) were collected from adajan BMR Microbiology and athwalines area, in plastic bags and transported carefully to the laboratory. The collected plant material was authenticated at the Department of Botany, P.T.Science College, Athwalines, Surat. Fresh plant materials were used for isolation work to reduce the chance of contamination. The collected plant leaves were subjected to surface sterilization within a few hours after sampling. Surface Sterilization All the leaf samples were subjected to a three-step surface sterilization procedure according to the method described by Petrini. Initially all the leaves were washed in running tap water for 10 minutes to remove, soil particles and adhered debris, and finally washed with distilled water. This was followed by washing in 95% ethanol for 1 minute, in 2% sodium hypochlorite for 10 seconds and in 95% ethanol for 1 minute. Finally, the leaves were washed in sterile distilled water for 2 minutes [2] Table 1: selected medicinal plants for isolation of endophytic fungi). Sample no. Scientific name Local name 1 Ocimum tenuiflorum Tulsi 2 Epipremnum aureum Money plant 3 Azadirachta indica Neem 4 Piper betle Naagarvel paan 5 Catharanthus roseus Sadabahar 6 Ficus religiosa Pipdo 7 Musa acuminata Banana 8 Ficus Benghalensis Vad 9 Ficus racemosa Umaraa 10 Calotropis procera Aankado Isolation of Endophytic Fungi from Plant Leaves After surface sterilization, following two methods [2] were used for isolation of endophytes. Method 1 The leaves were crushed with sterile distilled water using sterile mortar and pestle. About 1 ml of crushed sample was serially diluted up to 10-5 dilutions using 12.5 mm potassium phosphate buffer (ph 7.1). About ml of aliquot from 10-2 to 10-5 dilutions were taken and spread on Potato dextrose agar media supplemented with chloramphenicol as an antibiotic (50µg/ml) and all the plates were incubated at 30 C [5]. Observation was carried out daily until the growth of endophytic fungi was observed. Method 2 The outer tissues were removed from the leaves by crushing it with the application of minimal pressure using sterile mortar and pestle and the inner tissues were excised and about 2 3 segments of crushed leaves were placed onto petri plates containing Potato dextrose agar media supplemented with chloramphenicol as an antibiotic (50µg/ml) and incubated at 30 C. Observation was carried out daily until the growth of endophytic fungi was observed. Purification, Selection and Preservation of Endophytic Fungi 2 After incubation, fungal colonies were selected and streaked on Potato dextrose agar plates and incubated at 30 C for about 72 hours. Morphological and Microscopical Characterization of Endophytic Fungi The identification procedure of endophytic fungi was based on morphology. The sample of fungal isolates were mounted on the sterile slides then it was stained with lactophenol cotton blue and examined in 40X light microscopy [15]. The twenty-five
3 isolated species were described according to their macroscopic features (i.e. the topography, texture and colour) as well as microscopic characteristics (i.e. the structure of hyphae, conidia and conidiophores) [1]. Obtained data were then compared with the descriptions of fungi species in the identification of pathogenic fungi by Colin K. Campbell and matches were recorded [3][6][8][12]. Those cultures which failed to sporulate were grouped under mycelia sterilia. This is the common problem concerning with the identification of endophytes [13]. RESULTS A total of 25 morphologically distinct endophytic fungi were isolated. From the two methods used for the isolation of endophytic fungi, Method 2 showed better growth of endophytes compared to Method 1. The percentage occurrence of endophytic fungi varies with the host plants. (Fig. 1). The identification of fungal strains was done under the basis of macroscopic and microscopic observations of cultural characters (Fig. 2). (Figure:1) Overall Relative percentage Occurrence of endophytic fungi recorded in selected medicinal plants Ocimum tenuiflorum 12% 2 Epipremnum aureum Azadirachta indica 16% 8% 16% 8% Piper betle Catharanthus roseus Ficus religiosa Musa acuminata Ficus Benghalensis Ficus racemosa Calotropis procera (Figure:2) Morphotypic characterization of endophytic fungi by studying their Colony performance on the media PDA and by staining technique A: Aspergillus niger, B: Alternaria spp., C: Trichophyton spp., D: Geotrichum spp., E: Candida albicans, F: Aspergillus spp., G: Alternaria spp., H: Aureobasidium spp., I: Fusarium spp., J: Trichophyton spp., K: Trichophyton Violaceum, L: Exserohilum spp., M: Aspergillus spp., N: Aspergillus spp., O: Aspergillus spp., P: Candida kefyr, Q: Aspergillus spp., R: Curvularia spp., S: Aspergillus spp., T: Coccidioides spp., U: Aspergillus spp., V: Trichophyton spp., W: Bipolaris spp., X: Aspergillus spp. A B 3 C D \
4 E F G H I J K L M N O P 4
5 Q R S T U V W X The Majority of the recovered endophtytes belong to the Ascomycota. These fungi belong to four Ascomycota classes. The Aspergillus, Trichophyton, Coccidioides species belong to Eurotiomycetes, Curvularia, Alternaria, Aureobasidium, Exserohilum, Bipolaris species belong to Dothideomycetes, Geotrichum, Candida species belong to Saccharomycetes and Fusarium species belong to Sordariomycetes class.(fig.3) (Figure:3) Endophytic isolates from different fungal classes 5 Eurotiomycetes Dothideomycetes Saccharomycetes Sordariomycetes Mycelia sterilia
6 6 DISCUSSION The plant tissues, specially leaves and stems are excellent reservoirs for endophytic fungi (Petrini, 1991; Bokhary et al., 2000). Environmental factors such as temperature, rainfall and atmospheric humidity and their effect on host plant made the variations in occurrence of endophytic fungi and their colonization frequency (Selvanathan et al., 2011). Therefore, in present survey of endophytic fungi isolated from ten medicinal plants from Gujarat region have variations in endophytes and their relative percentage occurrence. K.P.Suradkar et al (2014) investigating Seasonal diversity of endophytic fungi. The leaf isolates mostly fell into Cladosporium spp., Verticillium spp., Arthrinium spp., Colletotrichum spp. and Aspergillus spp. from Ocimum tenuiflorum and Calotropis procera respectively. In our study we got Trichophyton spp., Bipolaris spp., Aspergillus spp., Geotrichum spp., Alternaria spp. and Candida spp. Fungal endophytes were isolated from leaves of Azadirachta indica and Piper betle in nashik (Sidhu et al 2015). Cladosporium spp., Curvularia spp. and Colletotrichum spp. were obtained as endophytic fungi while we found Fusarium spp., Trichophyton spp., Exserohilum spp and one mycelia sterilia from the same plants. V.H. Sunitha et al (2013) study the diversity of microbial community associated with healthy different plant parts of the Catharanthus roseus such as leaves, midrib, petiole and stems in Bangalore. They recovered and identified fungal endophytes, which were closely related to species of the following genera Colletotrichum spp., Drechsclera spp., Cladosporium spp., Myrothecium spp while we recovered only Aspergillus spp. Beatriz et al (2017) conducted endophytic fungi in economically important plants in Brasil. Xylaria spp., Colletotrichum spp. and Cordana spp. were isolated from Musa acuminata and we found one candida spp. from the same. Rasika et al (2016) isolate and identified endophytic fungi from selected medicinal plants like Ficus religiosa, Ficus benghalensis and Ficus racemosa in Akola. The isolates belonged to Alternaria spp., Rhizopus spp., Curvularia spp. and Trichoderma spp. In our study we got curvularia spp. as same but another two endophytes Aspergillus spp. and Coccidioides spp. were different. S. Shafique et al (2014) examined endophytic fungi from infected leaf of Epipremnum aureum showing necrotic spots in Punjab and isolted Alternaria ochroleuca and we found same Alternaria spp. and Aureobasidium spp as an endophytes from the healthy leaves of that plant. Most of these genera including Curvularia, Aspergillus and Fusarium are common as plant pathogenic fungi though nonpathogenic endophytic forms of these also exist. Some reports have stated that endophytes remain latent, with symptomless nature inside the host plant only until the environmental conditions are favourable for the fungus to turn into aggressive saprophytes or opportunistic pathogens. The fungi in the current study were isolated from a healthy, symptomless plant after thorough surface sterilization, which confirm the isolated fungi were in the endophytic form during the isolation period [4]. CONCLUSION This investigation evaluated the diversity of endophytic fungi inhabiting various medicinal plant leaves. Twenty-five species of endophytic fungi were isolated and identified BMR Microbiology from leaves which belong to Alternaria spp., Trichophyton spp., Geotrichum spp., Candida albicans, Aspergillus spp., Aureobasidium spp., Fusarium spp., Exserohilum spp., Candida kefyr, Curvularia spp., Coccidioides spp., Bipolaris spp. One unidentified isolates were group to mycelia sterilia fungi which was morphological fungal types, but not forming true spores. Aspergillus had the highest frequency in this study and Fusarium was the second most frequent endophytic group. Almost fourteen isolates belong to the class Eurotiomycetes, six belong to Dothideomycetes, three belong to Saccharomycetes and one belong to Sordariomycetes. This study is based mainly on macroscopic and microscopic characterization of the isolated fungi which does not allow the identification of abundantly isolated sterile mycelia. In the future studies, we should adopt many molecular methods which help in identification of non-sporulating fungal isolates. REFERENCES [1]. Al-mahi Idris, Ietidal Al-tahir and Eihab Idris, Antibacterial activity of endophytic fungi extracts from the medicinal plant Kigelia Africana. Egypt. Acad. J. Biolog. Sci. 2013, 5(1): 1-9. [2]. Basha N.S., Ogbaghebriel A., Yemane K., Zenebe M., Isolation and screening of endophytic fungi from Eritrean traditional medicinal plant Terminalia brownii leaves for antimicrobial activity. International Journal of Green Pharmacy 2012, 6: [3]. Colin K. Campbell, Elizabeth M. Johnson, and David W. Warnock. Identification of Pathogenic Fungi, Second Edition. Health Protection Agency, 2013 by Blackwell Publishing Ltd. [4]. Dissanayake R.K., Ratnaweera P.B., Williams D.E., Wijayarathne C.D., Wijesundera R., Andersen R.J., Silva E.D., Antimicrobial activities of mycoleptodiscin B isolated from endophytic fungus Mycoleptodiscus sp. of Calamus thwaitesii Becc. Journal of Applied Pharmaceutical Science 2016, 6 (01): [5]. Gupta D., Mandal R., Singh J.P. and Sandhu S.S., Screening of endophytic fungi isolated from some medicinal plants in jabalpur region for antibacterial activity. World Journal of Pharmacy and Pharmaceutical Sciences 2014, 3: [6]. Kalyanasundaram I., Nagamuthu J., Muthukumaraswamy S., Antimicrobial activity of endophytic fungi isolated and identified from salt marsh plant in Vellar Estuary. Journal of Microbiology and Antimicrobials 2015, 7: [7]. Karunai S.B. and Balagengatharathilagam P., Isolation and screening of endophytic fungi from medicinal plants of virudhunagar district for antimicrobial activity. International Journal of Science and Nature 2014, 5: [8]. Nur Amin, Muslim S., Muhammad J., Asman and Muh.Said Baco, Isolation and identification of endophytic fungi from cocoa plant resistante VSD M.05 and cocoa plant Susceptible VSD M.01 in South Sulawesi, Indonesia. International Journal of Current Microbiology and Applied Sciences 2014, 3(2): [9]. Patil M.G.,Pagare J.,Patil S.N.,Sidhu A.K., Extracellular Enzymatic Activities of Endophytic Fungi Isolated from Various Medicinal Plants. International Journal of Current Microbiology and Applied Sciences 2015, 4: [10]. Selim KA, El-Beih AA, AbdEl R.TM and Diwany AI, Biology of Endophytic Fungi. Current Research in Environmental & Applied Mycology 2012, 2: [11]. Singh D.,Rathod V.,Singh A.K.,Joshi R., Nagaratna H, Azmathunnisa, Avinash B., Antibacterial Activity and
7 Phytochemical Analysis of the Crude Extracts of Endophytic Fungus, Alternaria sp. from the Medicinal Plant Euphorbia hirta (L). International Journal of Green Chemistry and Bioprocess 2015, 5: [12]. Song J.J., Pongnak W. and Soytong K., Isolation and identification of endophytic fungi from 10 species palm trees. International Journal of Agricultural Technology 2016, 12(2): [13]. V.H. Sunitha, D. Nirmala Devi and C. Srinivas, Extracellular Enzymatic Activity of Endophytic Fungal Strains Isolated from Medicinal Plants. World Journal of Agricultural Sciences 2013, 9 (1): [14]. Venkatesan S., Ramar G., Kathirvelu B., Naif Dhabi A., Veeramuthu D., Biological properties of Endophytic Fungi. Brazilian archives of biology and technology 2016, 59:1-7. [15]. Yadav R., Singh A.V., Joshi S. and Manish K., Antifungal and enzyme activity of endophytic fungi isolated from Ocimum sanctum and Aloe vera. African Journal of Microbiology Research 2015, 9(29): BMR Microbiology 7
ISOLATION, FREQUENCY DISTRIBUTION AND DIVERSITY OF NOVEL FUNGAL ENDOPHYTES INHABITING LEAVES OF CATHARANTHUS ROSEUS
Int. J. LifeSc. Bt & Pharm. Res. 2013 Prerna Momsia and Teena Momsia, 2013 Research Paper ISSN 2250-3137 www.ijlbpr.com Vol. 2, No. 4, October 2013 2013 IJLBPR. All Rights Reserved ISOLATION, FREQUENCY
More informationThis thesis is an outcome of efforts made on studies on the diversity, ecology and
Chapter V SUMMARY This thesis is an outcome of efforts made on studies on the diversity, ecology and activities of microfungi associated with some medicinal plants of the forests of Western Ghats in Goa,
More informationINTRODUCTION budding, binary fission hyphae mycelium Figure 1.
INTRODUCTION Although most of our work in this lab is done on bacteria, fungi are nonetheless an important aspect in microbiology. Besides being important food providers, fungi play central roles in recycling
More informationDetection of Biocontrol Agents from Contaminated Fungal Culture Plates. Abstract
Plant Environment Development 4(2):21-25, 2015 (July) Department of Botany, University of Rajshahi e-mail: plant.environ.dev@ru.ac.bd Print ISSN 1994-1501 Online ISSN 2311-3529 Short Communication Detection
More informationJournal of Chemical and Pharmaceutical Research, 2018, 10(5): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2018, 10(5): 84-88 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Fungal Biodiversity Study of Varied Soil Samples
More informationMicroflora of Leaf Surface in Relation to Virus Infection
Microflora of Leaf Surface in Relation to Virus Infection By R. R. Mishra and V. B. Srivastava Department of Botany, University of Gorakhpur, Gorakhpur, U. P. (India) The study of leaf surface microflora
More informationEfficacy of Nano Particles from Chaetomium cupreum to Control Phytophthora spp. Causing Root Rot of Durian
International Journal of Agricultural Technology 2017 Vol. 13(7.1):1295-1300 Available online http://www.ijat-aatsea.com ISSN 1686-9141 Efficacy of Nano Particles from Chaetomium cupreum to Control Phytophthora
More informationUnit G: Pest Management. Lesson 2: Managing Crop Diseases
Unit G: Pest Management Lesson 2: Managing Crop Diseases 1 Terms Abiotic disease Bacteria Biotic disease Cultural disease control Disease avoidance Disease resistance Disease tolerance Fungi Infectious
More informationUnit D: Controlling Pests and Diseases in the Orchard. Lesson 5: Identify and Control Diseases in the Orchard
Unit D: Controlling Pests and Diseases in the Orchard Lesson 5: Identify and Control Diseases in the Orchard 1 Terms Abiotic disease Bacteria Biotic diseases Cultural disease control Disease avoidance
More informationIsolation optimization of bacterial endophytes from cucumber plants and evaluation of their effects on growth promotion and biocontrol
Isolation optimization of bacterial endophytes from cucumber plants and evaluation of their effects on growth promotion and biocontrol Ozaktan H., Gül A., Çakır B., Yolageldi L., Akköprü A., Fakhraei D.,
More informationReport of Blue Mould Rot of Rhizome of Tiger Lily (Gloriosa superb Linn)
EUROPEAN ACADEMIC RESEARCH Vol. II, Issue 3/ June 2014 ISSN 2286-4822 www.euacademic.org Impact Factor: 3.1 (UIF) DRJI Value: 5.9 (B+) Report of Blue Mould Rot of Rhizome of Tiger Lily (Gloriosa superb
More informationFinal Report. Minor Research Project. Studies of Rhizosphere, Rhizoplane and Phyllosphere Microorganism with Mangrove. plant in Mumbai Coast
Final Report Of Minor Research Project On Studies of Rhizosphere, Rhizoplane and Phyllosphere Microorganism with Mangrove plant in Mumbai Coast Sponsored By University Grant Commission Principal Investigator:
More informationIN-VITRO EVALUATION OF TRICHODERMA ISOLATES AGAINST MAJOR SOIL BORNE PATHOGENS IN GROUNDNUT (ARACHIS HYPOGAEA L)
International Journal of Agricultural Science and Research (IJASR) ISSN (P): 2250-0057; ISSN (E): 2321-0087 Vol. 7, Issue 3, Jun 2017, 319-326 TJPRC Pvt. Ltd. IN-VITRO EVALUATION OF TRICHODERMA ISOLATES
More informationIsolation and identification of phylloplane and endophytic fungi from one ornamental plant, Mangifera indica
International Journal of TechnoChem Research ISSN:2395-4248 www.technochemsai.com Vol.01, No.03, pp 188-192, 2015 Isolation and identification of phylloplane and endophytic fungi from one ornamental plant,
More information(DMB 01) M.Sc. (Previous) DEGREE EXAMINATION, DECEMBER First Year. Microbiology. Paper I INTRODUCTION TO MICROORGANISMS
wk 7 (DMB 01) Paper I INTRODUCTION TO MICROORGANISMS PART A (5 8 = 40 marks) 1. Explain the growth of microbiology in the twentieth century. 2. Describe the structure of eukaryotic cell with a neat-labeled
More informationIdentification of culturable endophytes isolated from apple tissues with antagonism towards Neonectria ditissima
Identification of culturable endophytes isolated from apple tissues with antagonism towards Neonectria ditissima Jing Liu, Hayley Ridgway & Eirian Jones Background Apple production in NZ widely cultivated
More informationFully approved by The South African Department of Agriculture, Forestry & Fisheries Registration Number: B4807
ExploGrow is a revolutionary 100% organic biofertiliser in a league of its own, comprising 17 highly complex micro-organisms, environmentally friendly soil ameliorant and plant growth stimulant, with increased
More informationPelagia Research Library. Antimicrobial activity of pesticide adapted cyanobacteria on fungal pathogens of rice
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2011, 1 (4):50-54 ISSN: 2248 9215 Antimicrobial activity of pesticide adapted cyanobacteria on fungal pathogens
More informationSymbiotic Fungal Endophytes that Confer Tolerance for Plant Growth in Saline and Dry Soils Zakia Boubakir, Elizabeth Cronin, Susan Kaminskyj
Symbiotic Fungal Endophytes that Confer Tolerance for Plant Growth in Saline and Dry Soils Zakia Boubakir, Elizabeth Cronin, Susan Kaminskyj Department of Biology University of Saskatchewan 1 Outline Background
More informationHorizontal gene transfer from trees to ectomycorrhizal fungi: Lessons from laboratory and host plant liberation experiments
Horizontal gene transfer from trees to ectomycorrhizal fungi: Lessons from laboratory and host plant liberation experiments Dr. Uwe Nehls 1,2, Dr. Chi Zhang 1, Dr. Mika Tarkka 1, Andrea Bock 1 1: University
More informationKingdom Fungi. 1. Student will be able to describe the characteristic features in the kingdom Fungi.
Kingdom Fungi Molds, Sac Fungi, Mushrooms, and Lichens Essential Question(s): What makes fungi have their own kingdom? Objectives: 1. Student will be able to describe the characteristic features in the
More informationWorksheet for Morgan/Carter Laboratory #13 Bacteriology
Worksheet for Morgan/Carter Laboratory #13 Bacteriology Ex. 13-1: INVESTIGATING CHARACTERISTICS OF BACTERIA Lab Study A: Colony Morphology Table 13.1 Characteristics of Bacterial Colonies Name of Bacteria
More informationLong Term Preservation of Commercial Important Fungi in Glycerol at 4 C
International Journal of Biological Chemistry 9 (2): 79-85, 2015 ISSN 1819-155X / DOI: 10.3923/ijbc.2015.79.85 2015 Academic Journals Inc. Long Term Preservation of Commercial Important Fungi in Glycerol
More informationFUNGAL ENDOPHYTES IN THREE MEDICINAL PLANTS OF LAMIACEAE
Acta Microbiologica et Immunologica Hungarica, 56 (3), pp. 243 250 (2009) DOI: 10.1556/AMicr.56.2009.3.4 FUNGAL ENDOPHYTES IN THREE MEDICINAL PLANTS OF LAMIACEAE D. BANERJEE*, S. MANNA, S.MAHAPATRA and
More informationPreeti Juyal 1*, Vandana Shrivastava 2, Abhishek Mathur 3
International Journal of Scientific and Research Publications, Volume 7, Issue 7, July 2017 590 Antimicrobial activity of Endophytes from aerial and non aerial parts of Calotropis procera against Pathogenic
More informationFUNGAL AIRSPORA OVER THE SUGAR CANE FIELDS AT NASHIK SAMPLER
Research Article Ahire PP,, 2013; Volume 2(2): 11-15 ISSN: 2277-8713 FUNGAL AIRSPORA OVER THE SUGAR CANE FIELDS AT NASHIK USING TILAK AIR SAMPLER AHIRE P. P. 1, SHINDE H. P 2, GOSWAMI D. B. 3 KADAM V.
More informationMorphological and Cultural Studies of Sclerotium rolfsii Sacc. causing Foot Rot Disease of Tomato
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 3 (2017) pp. 1146-1153 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.603.133
More informationGreen Synthesis of Silver Nanoparticles and Their Antimicrobial Activity against Gram Positive and Gram Negative Bacteria
International Journal of Biotechnology and Bioengineering Research. ISSN 2231-1238, Volume 4, Number 4 (2013), pp. 341-346 Research India Publications http://www.ripublication.com/ ijbbr.htm Green Synthesis
More informationWorking with Mycorrhizas in Forestry and Agriculture
Working with Mycorrhizas in Forestry and Agriculture SUB Gdttingen 206 384661 Mark Brundrett, Neale Bougher, Bernie Dell, Tim Grove and Nick Malajczuk CONTENTS Chapter I. INTRODUCTION 1.1. MYCORRHIZAL
More informationEffect of host plant, cultivation media and inoculants sources on propagation of mycorrhizal fungus Glomus Mossae
EUROPEAN ACADEMIC RESEARCH Vol. V, Issue 12/ March 2018 ISSN 2286-4822 www.euacademic.org Impact Factor: 3.4546 (UIF) DRJI Value: 5.9 (B+) Effect of host plant, cultivation and inoculants sources on propagation
More informationQUANTIFYING VESICULAR-ARBUSCULAR MYCORRHIZAE: A PROPOSED METHOD TOWARDS STANDARDIZATION*
W. (1981)87, 6-67 6 QUANTIFYING VESICULAR-ARBUSCULAR MYCORRHIZAE: A PROPOSED METHOD TOWARDS STANDARDIZATION* BY BRENDA BIERMANN Department of Botany and Plant Pathology, Oregon State University, Corvallis,
More informationMicrobiology & Plant diversity I (Bacteria, Virus, Applied Microbiology, Algae, Fungi, Lichens and Plant Pathology) Category L T P Credit PL18C01
PL18C01 Microbiology & Plant diversity I (Bacteria, Virus, Applied Microbiology, Algae, Fungi, Lichens and Plant Pathology) Category L T P Credit CORE 86 4-6 Preamble To study the characteristics and life
More informationEffects of Smoke on Pathogens and Other Fungi
Effects of Smoke on Pathogens and Other Fungi JOHN R. PARMETER, JR. AND BJARNE UHRENHOLDT Department of Plant Pathology, University of California, Berkeley 94720 ACCUMULATING evidence indicates that western
More informationDiversity of Endophytic Fungi Isolated From Plant Leaves of Deciduous Dipterocarp Forest in Tak Province
Kasetsart J. (Nat. Sci.) 43 : 182-188 (2009) Diversity of Endophytic Fungi Isolated From Plant Leaves of Deciduous Dipterocarp Forest in Tak Province Krittapong Orachaipunlap 1 *, Sophon Roengsumran 2
More informationOntario Science Curriculum Grade 9 Academic
Grade 9 Academic Use this title as a reference tool. SCIENCE Reproduction describe cell division, including mitosis, as part of the cell cycle, including the roles of the nucleus, cell membrane, and organelles
More informationJournal of Chemical and Pharmaceutical Research, 2017, 9(3): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 217, 9(3):13-19 Research Article ISSN : 97-7384 CODEN(USA) : JCPRC Studies on Biodiversity of Phylloplane and Endophytic
More informationCharacterization and biosynthesis of Silver nanoparticles using a fungus Aspergillus niger
International Letters of Natural Sciences Online: 2014-05-13 ISSN: 2300-9675, Vol. 15, pp 49-57 doi:10.18052/www.scipress.com/ilns.15.49 2014 SciPress Ltd., Switzerland Characterization and biosynthesis
More informationMORPHOLOGICAL, CULTURAL AND PATHOGENIC CHARACTERISTICS OF MACROPHOMINA PHASEOLINA ISOLATES FROM SUGAR BEET
MORPHOLOGICAL, CULTURAL AND PATHOGENIC CHARACTERISTICS OF MACROPHOMINA PHASEOLINA ISOLATES FROM SUGAR BEET Stojšin, V., Budakov, D., Bagi, F., Đuragin, N., Marinkov, R. Department for Environmental and
More informationRICINUS COMMUNIS LINN. AND THEIR ANTIBACTERIAL ACTIVITY
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article ISOLATION AND IDENTIFICATION OF ENDOPHYTIC FUNGI FROM RICINUS COMMUNIS LINN. AND THEIR ANTIBACTERIAL
More informationPRODUCTION OF SPORANGIA BY PHYTOPHTHORA CINNAMOMI IN PURE CULTURE
California Avocado Society 1969 Yearbook 53: 103-107 PRODUCTION OF SPORANGIA BY PHYTOPHTHORA CINNAMOMI IN PURE CULTURE G. A. Zentmyer and Dah-wu Chen Department of Plant Pathology, University of California,
More informationInternational Journal of Pharma and Bio Sciences
Research Article Pathology International Journal of Pharma and Bio Sciences ISSN 0975-6299 EFFECT OF VOLATILE METABOLITES OF PHYLLOPLANE FUNGI OF CHLOROPHYTUM TUBEROSUM AGAINST ITS FUNGAL PLANT PATHOGEN
More informationRhizosphere mycoflora of some leguminous crop plants
Available online at www.ijpab.com INTERNATIONAL JOURNAL OF PURE & APPLIED BIOSCIENCE ISSN: 2320 7051 Int. J. Pure App. Biosci. 3 (3): 262-266 (2015) ResearchArticle Rhizosphere mycoflora of some leguminous
More informationASSIGNMENT-1. M.Sc. ( Previous ) DEGREE EXAMINATION, MAY 2018 First Year MICROBIOLOGY Introduction Microorganisms
ASSIGNMENT-1 Introduction Microorganisms (DMB 01) Q1) Germ theory of diseases. Q2) Leeuwenhoek. Q3) Mycoplasmas. Q4) Rhizobium. Q5) T4 Q6) Viroids. Q7) Protozoa classification. ASSIGNMENT-2 Introduction
More informationASSOCIATION OF MICROFLORA WITH RUBBER (Hevea brasiliensis) AND THEIR BENEFICIAL ROLES
ASSOCIATION OF MICROFLORA WITH RUBBER (Hevea brasiliensis) AND THEIR BENEFICIAL ROLES Introduction: The rubber tree (Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg.), the most important source of natural
More informationProtists can be animal-like, plantlike, or funguslike.
SECTION 19.1 DIVERSITY OF PROTISTS Study Guide KEY CONCEPT Kingdom Protista is the most diverse of all the kingdoms. VOCABULARY protist Protists can be animal-like, plantlike, or funguslike. 1. Are protists
More informationMicrobiology BIOL 202 Lecture Course Outcome Guide (COG) Approved 22 MARCH 2012 Pg.1
Microbiology BIOL 202 Lecture Course Outcome Guide (COG) Approved 22 MARCH 2012 Pg.1 Course: Credits: 3 Instructor: Course Description: Concepts and Issues 1. Microbial Ecology including mineral cycles.
More informationDiversity of Endophytes in Various Plants from Woods Hole, MA
Diversity of Endophytes in Various Plants from Woods Hole, MA By Ivelisse Irizarry Universidad del Turabo/Rutgers University iveliz@gmail.com, iveliz@eden.rutgers.edu Introduction Various plants from different
More informationTHE USE OF ENDOPHYTIC FUNGI AS BIOPESTICIDE AGAINST DOWNY MILDEW PERONOSCLEROSPORA SPP. ON MAIZE
THE USE OF ENDOPHYTIC FUNGI AS BIOPESTICIDE AGAINST DOWNY MILDEW PERONOSCLEROSPORA SPP. ON MAIZE Nur Amin 1, La Daha 2, A. Nasruddin 3, M. Junaed 4, Andi Iqbal 5 Department of Plant Protection, Hasanuddin
More informationAssessment Schedule 2016 Biology: Demonstrate understanding of biological ideas relating to micro-organisms (90927)
NCEA Level 1 Biology (90927) 2016 page 1 of 5 Assessment Schedule 2016 Biology: Demonstrate understanding of biological ideas relating to micro-organisms (90927) Evidence Statement Question One No response
More informationBacterial spot of pepper and tomato
Website to brush up on bacterial diseases Bacterial spot of pepper and tomato http://www.apsnet.org/edcenter/intropp/lessons/prokaryotes/pages/bacterialspot.aspx Potato blackleg and soft rot http://www.apsnet.org/edcenter/intropp/lessons/prokaryotes/pages/blacklegpotato.aspx
More informationKILGORE COLLEGE BIOLOGY DEPARTMENT Biology 2421 Syllabus
COURSE: BIOL 2421 (4-3-4) TITLE: CATALOG DESCRIPTION: Microbiology and Pathology A study of the morphology, physiology, genetics, taxonomy and control of microorganisms. This course includes a study of
More informationAssessment of Endophytic Fungal Flora Responsible for Plant Growth Promotion and Survival in Sanganer Region of Rajasthan
Annals of Ecology and Environmental Science Volume 1, Issue 1, 2017, PP 64-68 Assessment of Endophytic Fungal Flora Responsible for Plant Growth Promotion and Survival in Sanganer Region of Rajasthan Kartikeya
More informationBacteria. The Three Types of Important Heterotrophic Bacteria
Bacteria Kingdom Monera Prokaryote (their genetic material is not bound with a membrane) Classified according to shape - Spherical (cocci) - Spiral - Rod Shaped -TWO TYPES: Heterotrophic (organism that
More informationNORTHERN ILLINOIS UNIVERSITY. Screening of Chemical Libraries in Search of Inhibitors of Aflatoxin Biosynthesis. A Thesis Submitted to the
NORTHERN ILLINOIS UNIVERSITY Screening of Chemical Libraries in Search of Inhibitors of Aflatoxin Biosynthesis A Thesis Submitted to the University Honors Program In Partial Fulfillment of the Requirements
More informationEndophytic hyphal compartmentalization is required for successful mycobiont-wheat interaction as revealed by confocal laser microscopy
Endophytic hyphal compartmentalization is required for successful mycobiont-wheat interaction as revealed by confocal laser microscopy Vladimir Vujanovic 1, Lobna Abdellatif 1, and Susan Kaminskyj 2 1
More informationChapter 8. Summarizing Discussion
Chapter 8 Summarizing Discussion Chapter 8 This thesis focuses on the ecology and pathogenicity of biovar 3 Dickeya sp. provisionally called D. solani, a blackleg and soft rot pathogen, recently introduced
More informationQ1) Germ theory of diseases. Q2) Louis Pasteur. Q3) Bacillus. Q4) Cyanobacteria. Q5) Viroids. Q6) Prions Q7) TMV Q8) T4
(DMB01) ASSIGNMENT - 1 Introduction Microorganisms Q1) Germ theory of diseases Q2) Louis Pasteur Q3) Bacillus Q4) Cyanobacteria Q5) Viroids Q6) Prions Q7) TMV Q8) T4 (DMB01) ASSIGNMENT - 2 Introduction
More informationPlant disease. Plant Diseases: Learning objectives: Plant Disease: Any physiological or structural abnormality that is harmful to the plant
Plant disease Plant Diseases: Identification and Control Melodie Putnam Extension Plant Pathologist Learning objectives: Difference between biotic and abiotic diseases and their manifestation Difference
More informationStudies on fungal endophytes and antibacterial activity of medicinal plant Bergenia ciliata (haw.) Sternb
2017; 3(1): 328-333 ISSN Print: 2394-7500 ISSN Online: 2394-5869 Impact Factor: 5.2 IJAR 2017; 3(1): 328-333 www.allresearchjournal.com Received: 15-11-2016 Accepted: 16-12-2016 Deepika Verma Anand Sagar
More informationFungal Identification Practical Manual Microbiology READ ONLINE
Fungal Identification Practical Manual Microbiology READ ONLINE If you are searched for the book Fungal identification practical manual microbiology in pdf form, then you have come on to correct site.
More informationCommercial microbial inoculants with endophytes (an overview)
Commercial microbial inoculants with endophytes (an overview) Matthias Döring INOQ GmbH, Germany Vorname Name www.inoq.de 1 Inquiries about products with ENDOPHYTES by: EU commission of agriculture German
More informationShort overview on microbial ecology of the vineyards
Short overview on microbial ecology of the vineyards Stéphane Compant Center for Health & Bioresources, AIT Austrian Institute of Technology GmbH, 3430 Tulln, Austria Stephane.Compant@ait.ac.at Summary
More informationManagement of Root Knot Disease in Rice Caused by Meloidogyne graminicola through Nematophagous Fungi
Management of Root Knot Disease in Rice Caused by Meloidogyne graminicola through Nematophagous Fungi Sobita Simon H.O.D., Plant Protection Department Allahabad Agricultural Institute, D.U. Post Box No.
More informationYear 09 Science Learning Cycle 5 Overview
e Year 09 Science Learning Cycle 5 Overview Learning Cycle Overview: Biology How do we keep your body healthy L01 4.3.1.1 Communicable (infectious) disease L02 4.3.1.2 Viral diseases L03 4.3.1.3 Bacterial
More informationAssessment of Microbial diversity in non-rhizosphere soil of forest nurseries in Southern Tamil Nadu, India
ISSN: 2319-7706 Volume 3 Number 6 (2014) pp. 454-458 http://www.ijcmas.com Original Research Article Assessment of Microbial diversity in non-rhizosphere soil of forest nurseries in Southern Tamil Nadu,
More informationOrchidaceae Vanillin.
INTRODUCTION Biological control of plant pathogens is considered as a potential control strategy in recent years, because chemical control results in accumulation of harmful chemical residues, which may
More informationDEPARTMENT OF MICROBIOLOGY. Microbiology Programme: Bachelor of Science (Honours) Microbiology
DEPARTMENT OF MICROBIOLOGY Microbiology Programme: Bachelor of Science (Honours) Microbiology Philosophy Microbiology is simply a natural science that deals with the study of microbes: their structure,
More informationVan Bael et al., Endophytic fungi increase the processing rate of leavesby leaf-cutting ants (Atta). Ecological Entomology
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Supporting Information available online Van Bael et al., Endophytic fungi increase the processing rate of leavesby leaf-cutting ants (Atta). Ecological Entomology
More informationTreat the Cause not the symptom
Treat the Cause not the symptom A few facts about Novozymes Biologicals Bu sin ess d ivisio n o f No vo zym es w it h it s o w n R& D, Manufacturing, Sales & Marketing, Administration Headquartered in
More informationfungi from Cuscuta reflexa and its host plants
Endophytic Fungal Diversity fungi from Cuscuta reflexa and its host plants T.S. Suryanarayanan*, G. Senthilarasu and V. Muruganandam PG and Research Department of Botany, Ramakrishna Mission Vivekananda
More informationFirst study on Antimicrobial activities of Endophytes Isolated from Aerial parts of Mentha Piperita
International Journal of Scientific and Research Publications, Volume 7, Issue 7, July 2017 90 First study on Antimicrobial activities of Endophytes Isolated from Aerial parts of Mentha Piperita Mukesh
More informationPlant and animal cells (eukaryotic cells) have a cell membrane, cytoplasm and genetic material enclosed in a nucleus.
4.1 Cell biology Cells are the basic unit of all forms of life. In this section we explore how structural differences between types of cells enables them to perform specific functions within the organism.
More informationANALYSIS OF MICROBIAL COMPETITION
ANALYSIS OF MICROBIAL COMPETITION Eric Pomper Microbiology 9 Pittsburgh Central Catholic High School Grade 9 Introduction Escherichia coli (E. coli) and Saccharomyces cerevisiae (Yeast) were grown together
More informationEFFECT OF MICROWAVES ON MOULDS ISOLATED FROM SURFACES
EFFECT OF MICROWAVES ON MOULDS ISOLATED FROM SURFACES short communication ELENA DAVID, ALINA LEAHU, ȘTEFAN OANCEA, MONICA MIRONESCU 1 Faculty of Agricultural Sciences, Food Industry and Environmental Protection,
More informationbelonging to the Genus Pantoea
Emerging diseases of maize and onion caused by bacteria belonging to the Genus Pantoea by Teresa Goszczynska Submitted in partial fulfilment of the requirements for the degree Philosophiae Doctoriae in
More informationfor GREENHOUSES GREENHOUSE Why are Mycorrhizae Important? Benefit to Plants
GREENHOUSE for GREENHOUSES Why are Mycorrhizae Important? Mycorrhizal fungi are essential to living soils, and allowed plants to colonize the surface of our planet around 450 million years ago. More than
More informationTHE IDENTIFICATION OF TWO UNKNOWN BACTERIA AFUA WILLIAMS BIO 3302 TEST TUBE 3 PROF. N. HAQUE 5/14/18
THE IDENTIFICATION OF TWO UNKNOWN BACTERIA AFUA WILLIAMS BIO 3302 TEST TUBE 3 PROF. N. HAQUE Introduction: The identification of bacteria is important in order for us to differentiate one microorganism
More informationFactors Affecting the Infection of Vesicular Arbuscular Mycorrhizal Fungi in Transformed Root Culture
Factors Affecting the Infection of Vesicular Arbuscular Mycorrhizal Fungi in Transformed Root Culture Poonpilai Suwanaritl, Savitri Ascharakul2, Omsub Nopamornbodi3 and Malee Suwana-adth4 I Department
More informationPrereq: Concurrent 3 CH
0201107 0201101 General Biology (1) General Biology (1) is an introductory course which covers the basics of cell biology in a traditional order, from the structure and function of molecules to the structure
More informationHigh Performance Biology
High Performance Biology Frequently Asked Questions (FAQ s) +61 3 5133 9118 What is Bacstim 100? Bacstim 100 is a microbial based product containing 5 different spore forming Bacillus spp. strains. The
More informationFungi with a Fun Guy: Feasts, Famines and the Fungus among Us Lauren Jackson v1.0
Fungi with a Fun Guy: Feasts, Famines and the Fungus among Us Lauren Jackson 2016-05-21 v1.0 Session Goals: 1. Investigate how fungi impact our food system a. Visualize fungal structures used to decay
More informationKeywords. Podosphaera leucotricha, scanning electron microscopy. Summary
Scanning electron microscopy of apple powdery mildew (Podosphaera leucotricha, Ell and Ev.) fungi infecting susceptible Jonathan apple cultivar leaf mesophyll ZS. JAKAB-ILYEFALVI 1 1 Fruit Research and
More informationChapter 31: Fungi. Student:
Chapter 31: Fungi Student: 1. Specialized symbiotic associations between the roots of plants and fungi are called A) lichens. B) hyphal associations. C) heterokaryotic junctions. D) mycorrhizae. E) a mycelium
More informationBIO-SYNERGY. Realise an unprecedented level of genetic. symbiot.com.au
BI-YERGY Realise an unprecedented level of genetic potential, plant quality and yield! UE A L A Y M B I R CDIIER UE EHACER 1 2 3 Mycorrhizal Root Inoculant Microbial utrient Additive FLIAR RAY Microbial
More informationStudies on biodiversity of phylloplane and endophytic fungi from different aged leaves of mangrove plant, Avicennia officinalis.
International Journal of ChemTech Research CODEN (USA): IJCRGG, ISSN: 0974-4290, ISSN(Online):2455-9555 Vol.10 No.6, pp 466-473, 2017 Studies on biodiversity of phylloplane and endophytic fungi from different
More informationSupporting information
Electronic Supplementary Material (ESI) for Organic & Biomolecular Chemistry. This journal is The Royal Society of Chemistry 209 Supporting information Na 2 S promoted reduction of azides in water: Synthesis
More informationChapter 37: Plant Nutrition - A Nutritional Network
Chapter 37: Plant Nutrition - A Nutritional Network Every organism continually exchanges energy and materials with its environment For a typical plant, water and minerals come from the soil, while carbon
More informationPlant Health and Protection
Plant Health and Protection National Collections of Arachnids, Fungi, Insects and Nematodes Rongi@arc.agric.za http://www.arc.agric.za/home.asp?pid=898 The ARC The Agricultural Research Council (ARC) is
More informationSurvey on Natural Occurrence of Endophytes in Maize (Zea mays L.) Ecosystem
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 01 (2018) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2018.701.303
More informationA.L.J. COLE Department of Botany, University of Canterbury, Christchurch, New Zealand INTRODUCTION
MAURI ORA, 1976, 4: 3-7 3 FUNGAL SPO R E GERMINATION INHIBITORS FROM WHEA T LEAVES A.L.J. COLE Department of Botany, University of Canterbury, Christchurch, New Zealand ABSTRACT When water droplets containing
More informationThe case for culturable air sampling for opportunistic fungi during healthcare construction
The case for culturable air sampling for opportunistic fungi during healthcare construction AIHCE 2007, Philadelphia, PA June 2007 Laurence Lee, CIH Pacific Industrial Hygiene LLC pacific.ih@comcast.net
More informationLecture 3. The fungal cell - II
Lecture 3 The fungal cell - II Asexual reproduction - formation of conidiospores (mitotic spores) Typical of Ascomycota Induction / suppression of conidiogenesis is controlled by both genetic and environmental
More informationGeorgia Performance Standards for Urban Watch Restoration Field Trips
Georgia Performance Standards for Field Trips 6 th grade S6E3. Students will recognize the significant role of water in earth processes. a. Explain that a large portion of the Earth s surface is water,
More informationScience at the University of Auckland. Presented by igem
Science at the University of Auckland Presented by igem Who are we? First of all Collecting Samples Why collect samples? Any ideas? To learn more about what you are studying To diagnose illnesses To collect
More informationIn vitro the effect of intestinal normal flora on some pathogenic bacteria.
In vitro the effect of intestinal normal flora on some pathogenic bacteria. Abstract: Dr.abbass shaker Ali adel Leena abd Al-Redha The effect of two types of intestinal bacterial normal floral ( and klebsiella)
More informationBiology I: Macaw Book Unit IV: Microbiology
Biology I: Macaw Book Unit IV: Microbiology Chapter 21 Fungus Fungus Lab Name: Date: Hour: Fungus Lab Pre-Lab Discussion Fungi are heterotrophic eukaryotes with cell walls that contain chitin. Most people
More informationAECC: Ability Enhancement Compulsory Course: DSC: Discipline Specific Course: DSE : Discipline Specific Elective
Telangana State Council of Higher Education, Govt. of Telangana B.Sc CBCS Common Core Syllabus for All Universities in Telanganas (wef 2016-2017). PROPOSED SCHEME FOR B.Sc PROGRAMME UNDER CHOICE BASED
More informationA Study of the Moss Parasite Eocronartium muscicola By: Alicia Knudson Advisor: Dr. Elizabeth Frieders
A Study of the Moss Parasite Eocronartium muscicola By: Alicia Knudson Advisor: Dr. Elizabeth Frieders Abstract The genus Eocronartium contains a single described species of parasitic fungus on moss plants
More informationIn vitro antifungal activity of Citrus aurantifolia Linn plant extracts against phytopathogenic fungi Macrophomina phaseolina
International Letters of Natural Sciences Online: 2014-04-12 ISSN: 2300-9675, Vol. 13, pp 70-74 doi:10.18052/www.scipress.com/ilns.13.70 2014 SciPress Ltd., Switzerland In vitro antifungal activity of
More informationTABLE OF CONTENTS CHAPTER NO. TITLE PAGE NO. LIST OF TABLES LIST OF FIGURES 1 INTRODUCTION AIM AND SCOPE OF THE PRESENT INVESTIGATION 7
viii TABLE OF CONTENTS ABSTRACT LIST OF TABLES LIST OF FIGURES iii xxiii xxviii 1 INTRODUCTION 1 1.1 AIM AND SCOPE OF THE PRESENT INVESTIGATION 7 2 LITERATURE REVIEW 8 2.1 AN OVERVIEW OF TEA 8 2.2 TEA
More information