2010 Art in Phytopathology

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1 2010 Art in Phytopathology Competition sponsored by the APS Graduate Student Committee

2 1 st Place in Art Blue Vesicle This is a depiction of a primary infection vesicle of Colletotrichum gloeosporioides f. sp. Salsolae (Cgs) as it ramifies within a cleared section of a host leaf.

3 2 nd Place in Art Tangled up in Blues Tangled Up in Blues (44" X 36") is mixed media on canvas and is a depiction of mycelial fragments of Cgs stained with lactophenol cotton blue, acridine orange and acid fuschin as seen under a compound microscope. Mixed media refers to the useage of acrylic and oil paints with the addition of charcoal.

4 3 rd Place in Art Orange Mycelial Space (48" X 36") This is mixed media on canvas and is a depiction of mycelial fragments of Cgs stained with lactophenol cotton blue, acridine orange and acid fuschin as seen under a compound microscope. Mixed media refers to the useage of acrylic and oil paints with the addition of charcoal.

5 Art Fungal Media for the Media A 5' pyrex plate with Malt Yeast Agar media was inoculated with several fungal species and left to grow for 30 days. The APS logo was cut out of the media to reveal a fungal art design. The plate sits upon a decaying polypore attached to a still living American Elm located on the University of Maine campus. The elm is named in memory of Richard J. Campana who inoculated the tree against the Dutch Elm disease.

6 Art Fungus Solaris The image is of fungi growing on a 5 inch diameter glass Pyrex plate. There are two different fungi: one that is yellow, and one that produces red secondary metabolites. The media, Malt Yeast Agar (MYA), is originally tan in color but was dyed by the fungi. The plate was held up to the sky as a backdrop.

7 Art Fungal Doodle Pen and paper - depicts fungal spores

8 Art The Miracle of Photosynthesis at Montery Bay It is meant to show carbon dioxide, water from the California sea breezes and sunshine combining miraculously to make a Monterey pine. These trees (Pinus radiata) are threatened by pitch canker (caused by Fusarium circinatum) in their tiny native range, the Monterey Peninsula of California, yet they are the most widely planted forest tree in the southern hemisphere. The quilt is made from cotton fabrics, cotton batting and glass beads.

9 1 st Place in Digitally Altered Epidemiologists Model Disease My artwork depicts mannequins modeling plant-disease dresses. The photos of the diseases were taken by myself and, in order from left to right, are of bacterial blight of strawberry (angular leaf spot), powdery mildew of grape, and Botrytis gray mold of strawberry.

10 2 nd Place in Digitally Altered Ring Nematodes Tubing and Slinky This image was created using copies from a single ring nematode, which is almost a C shaped plantparasitic nematode. A O ring was created using a copy of the first nematode arranged in a opposite way and slightly bent to create precise O shape. This ring was then stacked to create the tubing and slinky in Adobe Photoshop CS4. Ring nematodes are major pathogens of trees.

11 3 rd Place in Digitally Altered Stem Rust on Wheat Ear A digitally altered photograph of stem rust on a wheat ear

12 Digitally Altered An Ancient Foe to Mankind A digitally altered photograph of stem rust on a wheat stem

13 Digitally Altered Stem Rust on Wheat Stem A slightly digitally altered photograph of stem rust on a wheat stem

14 1 st Place in Microscopy Rainbow (10X) I marked the area I wanted to observe through the microscope with a purple Sharpie marker and noticed that the heat from the light bulb was boiling the dye I placed on the leaves (lactophenol cotton blue) and making the colors from the marker separate, creating a beautiful rainbow effect.

15 2 nd Place in Microscopy Blue Soybeans (20X) I marked the area I wanted to observe through the microscope with a blue Sharpie marker and noticed that it clearly stained the borders of the plant cells and stomates.

16 3 rd Place in Microscopy ET-The Extra Terrestrial This is a fluorescent image of a germinating blueberry pollen grain stained with a nitric oxide indicator dye. The two tentacle like structures are the pollen tubes while the eyes and mouth of this microscopic green alien are natural apertures on the pollen grain. This image was taken as a part of a comparative project of the conidia of a blueberry pathogen and pollen grains.

17 Microscopy Fishing for Spores (10X) Some areas of the soybean leaves appeared to be lighter than others. In this case, the lighter areas created the shape of a fish, and the blue eye is a stomate dyed with lactophenol cotton blue.

18 Microscopy Egg of Soybean Cyst Nematode Egg of soybean cyst nematode : The image shows SCN juvenile developing within the egg. Stylet is clearly visible.

19 Microscopy Second Stage Juvenile of Soybean Cyst Nematode The image shows second stage juvenile of soybean cyst nematode just hatched from an egg.

20 Microscopy Second Stage Juvenile of Soybean Cyst Nematode Second stage juvenile of soybean cyst nematode hatching from the egg

21 1 st Place in Nature The Fungal Con Artist This mysterious flower imposter is actually a fungus! Carefully excavated from a colonized blueberry fruit is the intact angel-wing structure of the mummy berry pathogen- Monilinia vaccinii-corymbosi. The shape and size of each petal of this flower like structure is delineated by the locules in the fruit. The structure is itself held together by the seeds and placenta (brown) in the center of the fruit.

22 2 nd Place in Nature Potatoes can Frown The photo is called Potatoes can frown and shows a potato tuber with symptoms of Potato Mop Top Virus infection.

23 3 rd Place in Nature Tobacco Mosaic Virus (TMV) Infected Tobacco Leaf This image shows artistic mosaic symptom, captured in flue-cured tobacco field using a Digital SLR camera. Image editing software Adobe Photoshop CS4 was used to create the TMV inscriptions from the mottled leaf. TMV was the first virus discovered on earth in 1883.

24 Nature Bubbling, oozing, slimy mound popping up; what has your golf course putting green got? An unknown slime mold spreading across a creeping bentgrass / annual bluegrass putting green.

25 Nature It s Alive! Gymnosporangium juniperi-virginianae in it's telial state on Eastern Red Cedar.

26 Nature Dwarf Mistletoe Over Lake Tahoe Female plants of western dwarf mistletoe (Arceuthobium sp.) on Jeffrey pine (Pinus jeffreyi Grev. & Balf.) along the shore of Lake Tahoe.

27 1 st Place in Wacky Vintage Necklace Crafted by nature s best, this necklace will be a perfect gift for the mycologist woman in your life, for any occasion! Guaranteed to be of fungal origin, this necklace is made solely with angel-wing structures painstakingly chiseled out from multiple blueberry fruits infected with Monilinia vacciniicorymbosi. Disclaimer: All sales are final and non-refundable. Keep necklace dry. The artist is not responsible for any growth of this necklace in high humidity and/or warm temperature.

28 2 nd Place in Wacky Strawberry Infected with Botrytis This image shows the humorous side of plant pathogen Botrytis, which grows very profusely on the fruit. This face of mine was merged into the infected strawberry in Adobe Photoshop CS4 to create a funny look with the fungal beard.

29 3 rd Place in Wacky Mike s Nose Phellinus robiniae on a tree in a private home in Blacksburg, VA. The owner has a good sense of humor!

30 Wacky Too Much Time at the Microscope

31 Wacky Rhizopus of Team Ruderal at the Soccer World Cup Micrograph of germinating spores of Rhizopus stolonifer.

32 BEST IN SHOW! Epidemiologists Model Disease

33 Congratulations to all the Winners! For everyone else who would like to compete next year, save your plant pathology art! Anyone who is a member of APS is eligible to compete.