Integrated North Sea zooplankton identification: A combination of morphology, DNA and proteome analyses towards a cost and time-effictive application
|
|
|
- Tamsyn Pearson
- 5 years ago
- Views:
Transcription
1 Integrated North Sea zooplankton identification: A combination of morphology, DNA and proteome analyses towards a cost and time-effictive application Silke Laakmann, Thomas Knebelsberger, Sabine Holst German Center for Marine Biodiversity Research (DZMB), Senckenberg Institute, Germany
2 MOTIVATION Accurate species identification as essential step in various studies Challenges in morphological species identification: - Young developmental stages (Holo- and Meroplankton, i.e. eggs, larvae) - Distinct life-history forms (i.e. metagenic species) - Phenotypic variations - Sibling / cryptic species (co-occurrence) Ensure species identification by using integrated morphological & molecular taxonomic approaches 2
3 MOTIVATION Accurate species identification as essential step in various studies Challenges in morphological species identification: - Young developmental stages (Holo- and Meroplankton, i.e. eggs, larvae) - Distinct life-history forms (i.e. metagenic species) - Phenotypic variations - Sibling / cryptic species (co-occurrence) Ensure species identification by using integrated morphological & molecular taxonomic approaches Need to establish a valid Reference Library due to increasing interests in molecular-based diversity approaches (i.e. high-throughput DNA sequencing techniques) 2
4 METHODOLOGY 1. Sampling and sorting (Calcofi 300, 500 µm, Apstein 150 µm mesh size) 2. Morphological identification (Specimens with diagnostic morphological characters) 3. Molecular analysis a) DNA sequence analyses - mitochondrial cytochrome c oxidase subunit I (COI) - nuclear ribosomal 18S rdna b) Proteomic Fingerprinting - MALDI-TOF Mass Spectrometry 4. Establishment of Reference Library by the comparison / integration of identifications 3
5 Calanoid Copepods Morphological identifications (333 specimens) 12 species based on morphology Acartia clausi Acartia bifilosa Acartia tonsa Anomalocera patersoni Calanus helgolandicus Centropages hamatus Centropages typicus Eurytemora affinis Isias clavipes Paracalanus parvus Pseudocalanus elongatus. Temora longicornis 4
6 Calanoid Copepods Molecular identifications Mitochondrial COI (136 specimens CVI F,M; 657bp) 13 species based on COI ( 11.7%) Siblings Acartia clausi Acartia bifilosa Acartia tonsa Anomalocera patersoni Calanus helgolandicus Centropages hamatus Centropages typicus Eurytemora affinis Isias clavipes Paracalanus parvus Pseudocalanus elongatus. Pseudocalanus moultoni Temora longicornis P. moultoni: New in the NE Atlantic or always there but not identified? 5
7 Calanoid Copepods Molecular identifications Nuclear 18S rdna (43 specimens CVI F,M; 1844 bp) 13 species based on 18S rdna ( 0.5%) Siblings Acartia clausi Acartia bifilosa Acartia tonsa Anomalocera patersoni Calanus helgolandicus Centropages hamatus Centropages typicus Eurytemora affinis Isias clavipes Paracalanus parvus Pseudocalanus elongatus. Pseudocalanus moultoni Temora longicornis 6
8 Proteomic Fingerprinting: MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometry) - Established in microbiology for routine identification of prokaryotic species & strains (e.g. industry, hospitals) - Comparison of species-specific mass spectra (2 20 kda) 7
9 Proteomic Fingerprinting: MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometry) - Established in microbiology for routine identification of prokaryotic species & strains (e.g. industry, hospitals) - Comparison of species-specific mass spectra (2 20 kda) Protein extraction: 10 min in matrix (cinnamic acid) Extract spotted on target plate & evaporates for several minutes Sample analysis: 96 samples ~ 1.5 h Comparative analyses: A) MALDI Biotyper (directly) B) Excel-based analyses, Primer6 C) R packages Benefit: Time- and cost-efficient!!! 7
10 Calanoid Copepods Molecular identifications Proteomic Fingerprinting (210 specimens: N,CI-VIF,M) 12 species (Acartia bifilosa was not analysed) Nauplii, CI-CV 8
11 Calanoid Copepods Molecular identifications Proteomic Fingerprinting (210 specimens: N,CI-VIF,M) 12 species (Acartia bifilosa was not analysed) Nauplii, CI-CV Comparable species identifications Proteome Fingerprints COI sequence analysis 8
12 Hydromedusae Morphological identifications (167 specimens) 08/ /2012 Helgoland Roads Jade & Elbe Estuary Obelia spp. Sarsia sp. Bougainvillia sp. Clytia sp. 18 species & 4 to genus only based on morphology Aglantha digitale Codonium proliferum Corymorpha nutans Ectopleura dumortierii Eirene viridula Eucheilota maculata Eutima gegenbauri Eutima gracilis Eutonina indicans Helgicirrha schulzei Lizzia blondina Leuckartiara octona Melicertum octocostatum Nemopsis bachei Rathkea octopunctata Sarsia tubulosa Stauridiosarsia gemmifera Tiaropsis multicirrata Bougainvillia sp. Sarsia sp. Clytia sp. Obelia sp. 9
13 Hydromedusae Molecular identifications Mitochondrial COI (167 specimens, 658 bp) 25 species based on COI ( 5.4%) 20 species 2 3 Aglantha digitale Codonium proliferum Corymorpha nutans Ectopleura dumortierii Eirene viridula Eucheilota maculata Eutima gegenbauri Eutima gracilis Eutonina indicans Helgicirrha schulzei Lizzia blondina Leuckartiara octona Melicertum octocostatum Nemopsis bachei Rathkea octopunctata Sarsia tubulosa Stauridiosarsia gemmifera Tiaropsis multicirrata Bougainvillia sp. Sarsia sp. Clytia sp.1 Clytia sp.2 Obelia sp.1 Obelia sp.2 Obelia sp.3 10
14 Hydromedusae Molecular identifications Mitochondrial COI (167 specimens, 658 bp) 25 species based on COI ( 5.4%) C. languida: New in the NE Atlantic or always there but not identified? & Clytia hemisphaerica Clytia languida 2 3 Kramp 1959 & Obelia dichotoma Obelia geniculata Obelia longissima 11
15 Hydromedusae & Ctenophora Molecular identifications Proteomic Fingerprinting (28 specimens) 9 species - Lower intra- than interspecific variability - High bootstrap values Aglantha digitale Corymorpha nutans Eirene viridula Eucheilota maculata Eutonina indicans Leuckartiara octona Nemopsis bachei Rathkea octopunctata Pleurobrachia pileus 12
16 Fish eggs Proteomic Fingerprinting Species-specific proteome spectra for the 3 different fish species eggs 13
17 Mixed Zooplankton Proteome Fingerprints for diverse Holo- & Meroplankton (i.e. Crustacea, Echinoderms, Polychaets, Appendicularians, Chaetognaths) - Lower intra- than interspecific variablity - High Bootstrap values 14
18 Mixed Zooplankton Proteome Fingerprints for diverse Holo- & Meroplankton (i.e. Crustacea, Echinoderms, Polychaets, Appendicularians, Chaetognaths) - Lower intra- than interspecific variablity - High Bootstrap values Possible Applications: Monitoring Detection of introduced species (e.g. rapid alert system) Biodiversity studies Ecological studies (i.e. species- & stage-specific vertical distribution) 14
19 CONCLUSIONS on Integrated North Sea zooplankton identification Integrated morphological & molecuar taxonomic approaches Consensus of morphological & molecular species status for most species New insights in diversity by uncovering sibling species 15
20 CONCLUSIONS on Integrated North Sea zooplankton identification Integrated morphological & molecuar taxonomic approaches Consensus of morphological & molecular species status for most species New insights in diversity by uncovering sibling species Reference data base ensures future North Sea species identifications Identify specimens with challenging morphological identification (i.e. young stages, different life-history forms, phenotypic variations, siblings) Analyse data generated by high-throughput DNA sequencing techniques 15
21 CONCLUSIONS on Integrated North Sea zooplankton identification Integrated morphological & molecuar taxonomic approaches Consensus of morphological & molecular species status for most species New insights in diversity by uncovering sibling species Reference data base ensures future North Sea species identifications Identify specimens with challenging morphological identification (i.e. young stages, different life-history forms, phenotypic variations, siblings) Analyse data generated by high-throughput DNA sequencing techniques Proteomic Fingerprinting as time- and cost-efficient species identification tool Monitoring (Biodiversity), Rapid alert system 15
22 Thank you for your attention Thanks to: Rebekka Schüller, Sven Hoffmann, Jasmin Renz-Gehnke, Viola Siegler, Sabine Gollner, Inga Mohrbeck, Peggy Weist, Pedro Martínez Arbizu (DZMB, Senckenberg Institute) Elena Markhaseva (Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russia) Gunnar Gerdts, René Erler, Astrid Cornils, Antje Wichels, Maarten Boersma (AWI) Maya Bode (Bremen University) Dominik Gloe, Klas Ove Möller, Bettina Walter, Janna Peters (IHF, Hamburg University) Funding This work is supported by the Federal Ministry of Education and Research (BMBF, Grant No. 03F0499A) and the Land Niedersachsen. Rebekka Schüller
1 Published June 30 I
Vol. 9: 1-11. 1982 1 MARINE ECOLOGY - PROGRESS SERIES Mar. Ecol. Prog. Ser. 1 Published June 30 I Zooplankton Communities in the Bristol Channel and Severn Estuary N. R. Collins and R. Williams Natural
Zooplankton Investigations in the Setonaikai (Inland Sea of Japan),IV.*
Journal of the Oceanographical Society of Japan Vol.25, No.3, pp.145 to 150, June 1969 Zooplankton Investigations in the Setonaikai (Inland Sea of Japan),IV.* Occurrence of Zooplankton in the Sea Regions
Dave Mackas 1 and Jackie King 2
Classification of Zooplankton Life History Strategies 1.0 0.8 Ctenophores 0.6 0.4 Medusae Pteropod s 0.2 Free-spawning Copepods Reproduction PC2 0.0-0.2-0.4 Euphausiid Chaetognaths Salp/Doliolid -0.6 Sacspawning
Predation by Aequorea victoria on other species of potentially competing pelagic hydrozoans
Vol. 72: 255-260, 1991 MARINE ECOLOGY PROGRESS SERIES Mar. Ecol. Prog. Ser. Published June 4 Predation by Aequorea victoria on other species of potentially competing pelagic hydrozoans Jennifer E. Purcell
DNA Barcoding and taxonomy of Glossina
DNA Barcoding and taxonomy of Glossina Dan Masiga Molecular Biology and Biotechnology Department, icipe & Johnson Ouma Trypanosomiasis Research Centre, KARI The taxonomic problem Following ~250 years of
VARIOUS DISTRIBUTION PATTERNS OF GREEN FLUO RESCENCE IN SMALLHYDROMEDUSAE
Kuroshio Biosphere Vol. 6, M ar. 2010, pp. 11-14 + 3 pls. VARIOUS DISTRIBUTION PATTERNS OF GREEN FLUO RESCENCE IN SMALLHYDROMEDUSAE By Shin KUBOTA 1 Abstract Twelve distributional patterns of fluorescent
DNA Barcoding Fishery Resources:
DNA Barcoding Fishery Resources: A case study in Shandong Costal Water Shufang Liu Laboratory of Molecular ecology of fishery resources Yellow Sea Fisheries Research Institute (YSFRI) Chinese Academy of
Amy Driskell. Laboratories of Analytical Biology National Museum of Natural History Smithsonian Institution, Wash. DC
DNA Barcoding Amy Driskell Laboratories of Analytical Biology National Museum of Natural History Smithsonian Institution, Wash. DC 1 Outline 1. Barcoding in general 2. Uses & Examples 3. Barcoding Bocas
2001 State of the Ocean: Chemical and Biological Oceanographic Conditions in the Newfoundland Region
Stock Status Report G2-2 (2) 1 State of the Ocean: Chemical and Biological Oceanographic Conditions in the Background The Altantic Zone Monitoring Program (AZMP) was implemented in 1998 with the aim of
DNA metabarcoding reveals diverse but selective diet of three-spined stickleback in a coastal ecosystem
DNA metabarcoding reveals diverse but selective diet of three-spined stickleback in a coastal ecosystem Jakubavičiūtė E* 1, Bergström U 2, Haenel Q 3, Bourlat SJ 4, Eklöf J 5 1 Nature Research Centre,
MARINE ECOLOGY PROGRESS SERIES Mar Ecol Prog Ser
Vol. 3: 3, 7 doi:.33/meps9 MARINE ECOLOGY PROGRESS SERIES Mar Ecol Prog Ser Published September 7 Population dynamics, growth and predation impact of the common jellyfish Aurelia aurita and two hydromedusae,
DNA barcoding is a technique for characterizing species of organisms using a short DNA sequence from a standard and agreed-upon position in the
DNA barcoding is a technique for characterizing species of organisms using a short DNA sequence from a standard and agreed-upon position in the genome. DNA barcode sequences are very short relative to
Zooplankton of the Okhotsk Sea: A Review of Russian Studies 1 ABSTRACT
Zooplankton of the Okhotsk Sea: A Review of Russian Studies 1 ABSTRACT The major Russian reports addressing Okhotsk Sea zooplankton published from 194 to 1999 are reviewed. Topics include a summary of
Predator avoidance by nauplii
MARINE ECOLOGY PROGRESS SERIES Vol. 7: 137 19, 3 Published February Mar Ecol Prog Ser Predator avoidance by nauplii Josefin Titelman 1,, 3, *, Thomas Kiørboe 1 1 Danish Institute for Fisheries Research,
Feeding: Metazoan Predators
Feeding: Metazoan Predators What do Metazoans Eat? Other metazoans (carnivores) e.g., chaetognaths eat copepods & copepods eat smaller crustaceans phytoplankton (herbivores) esp. larger ones like diatoms
IR Biotyper. Innovation with Integrity. Microbial typing for real-time epidemiology FT-IR
IR Biotyper Microbial typing for real-time epidemiology Innovation with Integrity FT-IR IR Biotyper - Proactive hospital hygiene and infection control Fast, easy-to-apply and economical typing methods
Seasonal Stability in Time Series of Zooplankton Abundance
Seasonal Stability in Time Series of Zooplankton Abundance TRAIANI STARI DOCTOR OF PHILOSOPHY THESIS Department of Mathematics and Statistics University of Strathclyde May 2010 Declaration This thesis
Interannual changes in the zooplankton community structure on the southeastern Bering Sea shelf and Chukchi Sea during summers of
Interannual changes in the zooplankton community structure on the southeastern Bering Sea shelf and Chukchi Sea during summers of 1991 29 22 27 Shiberia Chukchi Sea Alaska Pacific Summer Water Bering Sea
Effect of body size and temperature on generation time in zooplankton
Journal of Plankton Research Vol.22 no.2 pp.241 251, 2000 Effect of body size and temperature on generation time in zooplankton James F.Gillooly Department of Zoology, 430 Lincoln Drive, University of
Plant Names and Classification
Plant Names and Classification Science of Taxonomy Identification (necessary!!) Classification (order out of chaos!) Nomenclature (why not use common names?) Reasons NOT to use common names Theophrastus
Microbes usually have few distinguishing properties that relate them, so a hierarchical taxonomy mainly has not been possible.
Microbial Taxonomy Traditional taxonomy or the classification through identification and nomenclature of microbes, both "prokaryote" and eukaryote, has been in a mess we were stuck with it for traditional
Microbial Taxonomy. Slowly evolving molecules (e.g., rrna) used for large-scale structure; "fast- clock" molecules for fine-structure.
Microbial Taxonomy Traditional taxonomy or the classification through identification and nomenclature of microbes, both "prokaryote" and eukaryote, has been in a mess we were stuck with it for traditional
Ice matters. Arctic and Antarctic under-ice communities linking sea ice with the pelagic food web
Ice matters Arctic and Antarctic under-ice communities linking sea ice with the pelagic food web Hauke Flores, J. A. van Franeker, C. David, B. Lange, V. Siegel, E. A. Pakhomov, U. Bathmann... Hauke.flores@awi.de
Species Identification and Barcoding. Brendan Reid Wildlife Conservation Genetics February 9th, 2010
Species Identification and Barcoding Brendan Reid Wildlife Conservation Genetics February 9th, 2010 Why do we need a genetic method of species identification? Black-knobbed Map Turtle (Graptemys nigrinoda)
12. Tiselius, P. (1990) Foraging behaviour and grazing patterns of calanoid copepods (PhD Dissertation).
Peter Tiselius, publications 1. Tiselius, P.T. and Peterson, W.T. (1986), Life history and population dynamics of the chaetognath Sagitta elegans in Central Long Island Sound, J. Plankton Res., 8(1), 183-195.
Zooplankton of the Okhotsk Sea: A Review of Russian Studies 19
Zooplankton of the Okhotsk Sea: A Review of Russian Studies 19 Figure 18. Distribution of zooplankton biomass (mg m 3 ) near the western coast of Kamchatka in the 0-50 m layer: 1 = 100-500, 2 = 500-1,000,
Effects of elevated ph on marine copepods in mass cultivation systems: practical implications
Journal of Plankton Research academic.oup.com/plankt J. Plankton Res. (2017) 39(6): 984 993. First published online June 17, 2017 doi:10.1093/plankt/fbx032 Contribution to the Themed Section: Advances
Trophic ecology of pelagic fish species off the Iberian coast: diet overlap, cannibalism and intraguild predation
The following supplement accompanies the article Trophic ecology of pelagic fish species off the Iberian coast: diet overlap, cannibalism and intraguild predation S. Garrido*, A. Silva, J. Pastor, R. Dominguez,
POPULATION DYNAMICS AND PREDATION IMPACT OF THE INTRODUCED CTENOPHORE MNEMIOPSIS LEIDYI IN THE GULLMARS FJORD, WEST COAST OF SWEDEN
POPULATION DYNAMICS AND PREDATION IMPACT OF THE INTRODUCED CTENOPHORE MNEMIOPSIS LEIDYI IN THE GULLMARS FJORD, WEST COAST OF SWEDEN Lene Friis Møller & Peter Tiselius Dep. Of Marine Ecology Kristineberg
아산만해역중형동물플랑크톤의시공간적변동
The Sea Journal of the Korean Society of Oceanography Vol. 11, No. 1, pp. 1 10, February 2006 아산만해역중형동물플랑크톤의시공간적변동 Á Á 1 Á 2 û w y w 1Ÿ w y œw 2 sw w w lœw Spatio-temporal Variation of Mesozooplankton
Climate, zooplankton, and pelagic fish growth in the central Baltic Sea
ICES Journal of Marine Science, 6: 17e18 (5) doi:116/j.icesjms.5.4.1 Climate, zooplankton, and pelagic fish growth in the central Baltic Sea Christian Möllmann, Georgs Kornilovs, Marina Fetter, and Friedrich
Temperature Effects on the Egg Development Time and Hatching Success of Three Acartia Species (Copepoda: Calanoida) from the Strait of Malacca
Zoological Studies 5(5): 644-654 (202) Temperature Effects on the Egg Development Time and Hatching Success of Three Acartia Species (Copepoda: Calanoida) from the Strait of Malacca Teruaki Yoshida, *,
Microbial Taxonomy. Microbes usually have few distinguishing properties that relate them, so a hierarchical taxonomy mainly has not been possible.
Microbial Taxonomy Traditional taxonomy or the classification through identification and nomenclature of microbes, both "prokaryote" and eukaryote, has been in a mess we were stuck with it for traditional
Diversity and Functions of Protozoa in Soils
Diversity and Functions of Protozoa in Soils EcoFINDERS University of Cologne - AG Bonkowski 05 October 2011 Outlook Interaction studies Identification Diversity of Protozoa in Soils Single-celled, heterotrophic
Aquaculture Biology Laboratory
Aquaculture Biology Laboratory Faculty of Fisheries Nagasaki University Professor: Dr. Atsushi Hagiwara (hagiwara@net.nagasaki-u.ac.jp) Associate Professor: Dr. Yoshitaka Sakakura (sakakura@net.nagasaki-u.ac.jp)
Stepping stones towards a new electronic prokaryotic taxonomy. The ultimate goal in taxonomy. Pragmatic towards diagnostics
Stepping stones towards a new electronic prokaryotic taxonomy - MLSA - Dirk Gevers Different needs for taxonomy Describe bio-diversity Understand evolution of life Epidemiology Diagnostics Biosafety...
DNA sequence collection at CNR-IPSP: a resource for nematode identification
DNA sequence collection at CNR-IPSP: a resource for nematode identification Francesca De Luca CNR Istituto per la Protezione Sostenibile delle Piante, S.S. Bari email: francesca.deluca@ipsp.cnr.it EPPO
Production of Paralytic Shellfish Toxin (PST) by Alexandrium tamarense in response to Copepod grazing
Production of Paralytic Shellfish Toxin (PST) by Alexandrium tamarense in response to Copepod grazing mage must be placed here, if Viviana used. Bravo Carvajal Erik Selander Size 55x55 mm. Align right
Species composition and long-term dynamics of potentially toxic dinoflagellate species in benthic assemblages of Peter the Great Bay, Sea of Japan
Species composition and long-term dynamics of potentially toxic dinoflagellate species in benthic assemblages of Peter the Great Bay, Sea of Japan Marina Selina & Tatiana Morozova tatiana_morozova@mail.ru
Prey resource use by coexistent hydromedusae from Friday Harbor, Washington
Limnol. Oceanogr., 4(4), 00, 94 94 00, by the American Society of Limnology and Oceanography, Inc. Prey resource use by coexistent hydromedusae from Friday Harbor, Washington J. H. Costello Biology Department,
12. Tiselius, P. (1990) Foraging behaviour and grazing patterns of calanoid copepods (PhD Dissertation).
Peter Tiselius, publications 1. Tiselius, P.T. and Peterson, W.T. (1986), Life history and population dynamics of the chaetognath Sagitta elegans in Central Long Island Sound, J. Plankton Res., 8(1), 183-195.
Interannual variation in the composition of the assemblages of medusae and ctenophores in St Helena Bay, Southern Benguela Ecosystem*
SCI. MAR., 64 (Supl. 1): 123-134 SCIENTIA MARINA 2000 TRENDS IN HYDROZOAN BIOLOGY - IV. C.E. MILLS, F. BOERO, A. MIGOTTO and J.M. GILI (eds.) Interannual variation in the composition of the assemblages
Post-doc fellowships to non-eu researchers FINAL REPORT. Home Institute: Centro de Investigaciones Marinas, Universidad de La Habana, CUBA
Recipient: Maickel Armenteros Almanza. Post-doc fellowships to non-eu researchers FINAL REPORT Home Institute: Centro de Investigaciones Marinas, Universidad de La Habana, CUBA Promoter: Prof. Dr. Wilfrida
Curation of Zoological Collections
Entomologie II Managing and Curating Museum Collections II: Curation of Zoological Collections Part 1: Entomological Collections [im Rahmen des Workshopprogramms der Goethe Graduate Academy (GRADE), 19.
Diel Vertical Migration OCN 621
Diel Vertical Migration OCN 621 Outline Definition Who does it? How fast? Migration cues Why? Variations: seasonal, ontogenic, reverse Biogeochemical implications Diel Vertical Migration: Definitions Usually
Eric D. Stein Biology Department
Eric D. Stein Biology Department The Promise of Molecular Methods Faster answers Weeks vs. months Less expensive Better data Recognizing misidentifications Improving taxonomic keys Helping with difficult
Supplementary Information
1 Supplementary Information 1. Deriving values of scaling exponents of surface area versus body mass (b A ) by applying Euclidean geometry to additional dimensional measurements in the study species Given
Program Specific Outcomes: PSO of B. Sc., Zoology
Program Specific Outcomes: PSO of B. Sc., Zoology Demonstrated a broad understood of animal diversity, including knowledge of the scientific classification and evolutionary relationships of major groups
A Review of Russian Studies
A Review of Russian Studies Alexei I. Pinchuk Institute of Marine Science University of Alaska Fairbanks Fairbanks, Alaska A.J. Paul Seward Marine Center Laboratory Institute of Marine Science University
Tidal and subtidal currents influence deep copepod aggregations along a shelf-basin margin
The following supplement accompanies the article Tidal and subtidal currents influence deep copepod aggregations along a shelf-basin margin Kimberley T. A. Davies*, Tetjana Ross, Christopher T. Taggart
Taxonomy. Content. How to determine & classify a species. Phylogeny and evolution
Taxonomy Content Why Taxonomy? How to determine & classify a species Domains versus Kingdoms Phylogeny and evolution Why Taxonomy? Classification Arrangement in groups or taxa (taxon = group) Nomenclature
Distribution, Reproduction, and Transport of Zooplankton in the Western Arctic
University of Miami Scholarly Repository Open Access Dissertations Electronic Theses and Dissertations 2008-01-03 Distribution, Reproduction, and Transport of Zooplankton in the Western Arctic Leopoldo
Kam W.Tang, Hans G.Dam and Leah R.Feinberg Department of Marine Sciences, University of Connecticut, Groton, CT06340, USA
Journal of Plankton Research Vol.20 no.0 pp.97-987, 998 The relative importance of egg production rate, hatching success, hatching duration and egg sinking in population recruitment of two species of marine
High performance mass spectrometry - new methods & applications in life science
International Workshop High performance mass spectrometry - new methods & applications in life science August 26 th -28 th, 2009 Laboratory of Analytical Chemistry and Biopolymer Structure Analysis Guest
Progress in Oceanography
Progress in Oceanography 74 (07) 98 114 Progress in Oceanography www.elsevier.com/locate/pocean A decade of sampling in the Bay of Biscay: What are the zooplankton time series telling us? Luis Valdés a,
The L4 time-series: the first 20 years
INTRODUCTION The L4 time-series: the first 20 years THE WIDER CONTEXT Time-series observations are recognized as being increasingly important in an era of accelerated global change. In the North Atlantic,
Title NOTES ON SOME ANMAL PLANKTONS COLL FROM THE SEA OF JAPAN OFF SAN'N-D Author(s) Furuhashi, Kenzo Citation PUBLCATONS OF THE SETO MARNE BO LABORATORY (1952), 2(2): 257-260 ssue Date 1952-10-05 URL
Respiration and the activity of the respiratory electron transport system in marine zooplankton
Notes 549 Respiration and the activity of the respiratory electron transport system in marine zooplankton AbstracLRespiratory oxygen consumption and respiratory electron transport activity were measured
A Comparison of the Anomalous Ocean Conditions observed off the West Coast of Canada in 2014 and 2015.
A Comparison of the Anomalous Ocean Conditions observed off the West Coast of Canada in 2014 and 2015. Peter Chandler, Marie Robert, Moira Galbraith Institute of Ocean Sciences, Fisheries and Oceans Canada,
Intermittent plankton in marine turbulence
François G. Schmitt CNRS Research professor (DR) Director of the LOG, Laboratory of Oceanology and Geosciences, Wimereux, France Intermittent plankton in marine turbulence Leiden, Feb. 2016, Lorentz Center
Oceanographic assessment of the planktonic communities in the northeastern Chukchi Sea. Report for Survey year 2011
Oceanographic assessment of the planktonic communities in the northeastern Chukchi Sea Report for Survey year 211 Russell R Hopcroft, Jennifer Questel, Pallavi Hariharan, Chris Stark, Cheryl Clarke-Hopcroft
Resting eggs in free living marine and estuarine copepods
Downloaded from orbit.dtu.dk on: Apr 06, 2019 Resting eggs in free living marine and estuarine copepods Holm, Mark Wejlemann; Kiørboe, Thomas; Brun, Philipp Georg; Licandro, P.; Almeda, Rodrigo; Winding
Foraging behaviour of six calanoid copepods: observations and hydrodynamic analysis
Vol. 66: 23-33, 1990 MARINE ECOLOGY PROGRESS SERIES Mar. Ecol. Prog. Ser. Published September 6 Foraging behaviour of six calanoid copepods: observations and hydrodynamic analysis Peter ~iselius', Per
Determining the mass density of marine copepods and their eggs with a critical focus on some of the previously used methods
JOURNAL OF PLANKTON RESEARCH VOLUME 23 NUMBER 8 PAGES 859 873 2001 Determining the mass density of marine copepods and their eggs with a critical focus on some of the previously used methods TOR KNUTSEN
On the feeding of copepods on Thalassiosira partheneia from the Northwest African upwelling area
Vol. 11: 49-53, 1983 I MARINE ECOLOGY - PROGRESS SERIES Mar. Ecol. Prog. Ser. Published February 10 On the feeding of copepods on Thalassiosira partheneia from the Northwest African upwelling area Sigrid
The study of insect species diversity and modern approaches
The study of insect species diversity and modern approaches Humala A.E. Forest Research Institute Karelian Research Centre RAS Petrozavodsk, RUSSIA The main idea and aim of any research TO FILL THE GAPS
Individual-Based modeling of Copepods
Individual-Based modeling of Copepods Geoffrey Cowles Department of Fisheries Oceanography School for Marine Science and Technology University of Massachusetts-Dartmouth May 4, 2008 Outline Copepod Life
Phylogeography and genetic differentiation between Loxigilla noctis and L. barbadensis in the Lesser Antilles
Phylogeography and genetic differentiation between Loxigilla noctis and L. barbadensis in the Lesser Antilles Sophie Arnaud-Haond 1, Carla Daniel 2, Sébastien Motreuil 3, Julia Horrocks 2 & Frank Cézilly
Copepods as indicator species for comparing pelagic ecosystem models
Copepods as indicator species for comparing pelagic ecosystem models Outline Harold P. Batchelder Oregon State University Corvallis, OR hbatchelder@coas.oregonstate.edu Ecosystem Model Types Decisions
Wolbachia PCR: Discover the Microbes Within!
Wolbachia PCR: Discover the Microbes Within! Overview of the Wolbachia Lab Insect identification DNA extraction PCR? Wolbachia lecture Bioinformatics ACAGATGTC TTGTAATCCG GCCGTTGGT GGCATAGGG AAAGGACAT
MOLECULAR ANALYSIS OF JAPANESE ANISAKIS SIMPLEX WORMS
MOLECULAR ANALYSIS OF JAPANESE ANISAKIS SIMPLEX WORMS Azusa Umehara 1, 2, Yasushi Kawakami 2, Jun Araki 3, Akihiko Uchida 2 and Hiromu Sugiyama 1 1 Department of Parasitology, National Institute of Infectious
Biodiversity Division, National Institute for Agro-Environmental Sciences (Tsukuba, Ibaraki , Japan)
JARQ 48 (1), 35 40 (2014) http://www.jircas.affrc.go.jp Interaction between Introduced and Indigenous Parasitoids of Dryocosmus kuriphilus REVIEW Interaction between Torymus sinensis (Hymenoptera: Torymidae)
Individual-Based modeling of Copepods MAR524 Unit 11
Individual-Based modeling of Copepods MAR524 Unit 11 Geoffrey Cowles Department of Fisheries Oceanography School for Marine Science and Technology University of Massachusetts-Dartmouth Outline Copepod
CENTRAL ANALYTICAL AND APPLIED RESEARCH UNIT
List for Sample Preparation and Analysis Terms and Conditions: The following are the charges for the services currently offered by Central Analytical and Applied Research Unit (CAARU) in College of science.
Bruker Daltonics. EASY-nLC. Tailored HPLC for nano-lc-ms Proteomics. Nano-HPLC. think forward
Bruker Daltonics EASY-nLC Tailored HPLC for nano-lc-ms Proteomics think forward Nano-HPLC World-Class Performance with a Small Footprint Bruker Daltonics presents a nano-lc system, perfectly integrated
Technologies for sea bottom inhabitants' monitoring and Integral Costal Zones Management. Example of the Kandalaksha Bay, White Sea
Technologies for sea bottom inhabitants' monitoring and Integral Costal Zones Management. Example of the Kandalaksha Bay, White Sea Mikhail Tokarev, Roman Pevzner Transfer Technology Office of Lomonosov
Journal of Plankton Research. plankt.oxfordjournals.org
Journal of Plankton Research plankt.oxfordjournals.org J. Plankton Res. (2014) 36(1): 48 63. First published online September 30, 2013 doi:10.1093/plankt/fbt093 Morphological and molecular discrimination
Press Release: The Nobel Prize in Chemistry October 2002 The Royal Swedish Academy of Sciences has decided to award the Nobel Prize in
Press Release: The Nobel Prize in Chemistry 2002 9 October 2002 The Royal Swedish Academy of Sciences has decided to award the Nobel Prize in Chemistry for 2002 for the development of methods for identification
Macroevolution of Organismal Modularity and Integration. Gunther J. Eble Bioinformatik, Universität Leipzig
Macroevolution of Organismal Modularity and Integration Gunther J. Eble Bioinformatik, Universität Leipzig What is macroevolution? - evolution at and above the species level - large-scale phenotypic evolution
DNA BARCODING FRESHWATER ROTIFERA OF MEXICO
DNA BARCODING FRESHWATER ROTIFERA OF MEXICO Alma Estrella García Morales Manuel Elías Gutiérrez Zooplankton Laboratory El Colegio de la Frontera Sur, Chetumal, Mexico INTRODUCTION Systematics in Rotifera
LECTURE-13. Peptide Mass Fingerprinting HANDOUT. Mass spectrometry is an indispensable tool for qualitative and quantitative analysis of
LECTURE-13 Peptide Mass Fingerprinting HANDOUT PREAMBLE Mass spectrometry is an indispensable tool for qualitative and quantitative analysis of proteins, drugs and many biological moieties to elucidate
Mollusca: General Characteristics
Mollusca: General Characteristics Molluscan Taxonomic Classes Polyplacophora Cephalopoda Bivalvia 7,650 sp Other 5 Classes ~1100 Gastropoda Scaphopoda Gastropoda 40,000 sp and Aplacophora Monoplacophora
On the origin and propagation of Denmark Strait Overflow Water Anomalies in the Irminger Basin
On the origin and propagation of Denmark Strait Overflow Water Anomalies in the Irminger Basin Kerstin Jochumsen 1, Detlef Quadfasel 1, Manuela Köllner 1,2, Stephen Dye 3,4, Bert Rudels 5 and Heðinn Valdimarsson
Delineation of IRZs and PRZs in the German license area (CCZ): Criteria and characteristics
Delineation of IRZs and s in the German license area (CCZ): Criteria and characteristics Annemiek Vink, Carsten Rühlemann, Thomas Kuhn BGR Hannover, Germany Annika Janssen, Katja Uhlenkott DZMB Wilhelmshaven,
COPEPODS. A Thesis Presented to The Academic Faculty. Katherine Denise Rasberry
THE BEHAVIORAL EFFECT OF LABORATORY TURBULENCE ON COPEPODS A Thesis Presented to The Academic Faculty by Katherine Denise Rasberry In Partial Fulfillment of the Requirements for the Degree Master of Science
Predation efficiency in visual and tactile zooplanktivores
Limnol. Oceanogr., 49(1), 2004, 69 75 2004, by the American Society of Limnology and Oceanography, Inc. Predation efficiency in visual and tactile zooplanktivores Tom A. Sørnes 1 and Dag L. Aksnes Department
Dominant Types of Plankton. Phytoplankton Size. Diatoms. Diatoms. Each size has advantages & disadvantages Small cells
Dominant Types of Plankton Phytoplankton Diatoms dominant in neritic & upwelling zones Microflagellates dominant in oceanic zone Dinoflagellates dominant in transitional situations Zooplankton Protozoa
Studies on the small invertebrate plankton of the Sargasso Sea Ruth B6ttger*
HELGOL~NDER MEERESUNTERSUCHUNGEN Helgol~inder Meeresunters. 35, 369-383 (1982) Studies on the small invertebrate plankton of the Sargasso Sea Ruth B6ttger* Biologische Anstalt Helgoland (Zentrale); NotkestraBe
HOW TRAITS ARE INTERRELATED THROUGH. Survival. Fecundity. Survival and fecundity both depend on Feeding. To eat. To survive.
--5 What is a key trait? A property that is a main determinant of an individual s fitness HOW TRAITS ARE INTERRELATED THROUGH TRADE-OFFS IN ZOOPLANKTON* m m. mm.5 mm. mm Commonly used fitness measures:
Motivation for project:
Motivation for project: Shale Gas exploration and development requested of government by Oil Companies. Shale Gas Development Strategic Environment Assessment (SGD SEA) commissioned by DEA Biodiversity
Diversity of zooplankton in Parangipettai coastal waters, southeast coast of India
J. mar. biol. Ass. India, 45 (2) : 144-152, July - Dec., 2003 Diversity of zooplankton in Parangipettai coastal waters, southeast coast of India P. Santhanam and P. Perumal. Centre of Advanced Study in
Meeting Report: ALTER-Net Workshop about the Application of Molecular. Techniques to Study Biodiversity, Structure and Function of Planktonic
1 Meeting Report: ALTER-Net Workshop about the Application of Molecular Techniques to Study Biodiversity, Structure and Function of Planktonic Communities in Lakes at Blanes, Spain, February 15-16, 2007
Swimming and escape behavior of copepod nauplii: implications for predator-prey interactions among copepods
MARINE ECOLOGY PROGRESS SERIES Vol. 213: 203 213, 2001 Published April 4 Mar Ecol Prog Ser Swimming and escape behavior of copepod nauplii: implications for predator-prey interactions among copepods Josefin
Short-term variability in the assemblage of medusae and ctenophores following upwelling events in the southern Benguela ecosystem
MARINE ECOLOGY PROGRESS SERIES Vol. 220: 169 177, 2001 Published September 27 Mar Ecol Prog Ser Short-term variability in the assemblage of medusae and ctenophores following upwelling events in the southern
Identification of Stomatopod Larvae In Banten Bay, Indonesia Using DNA Barcoding
Identification of Stomatopod Larvae In Banten Bay, Indonesia Using DNA Barcoding Mariana D. Bayu Intan, M.Si. Dr. Abinawanto Drs. Wisnu Wardhana, M.Si. International Seminar on Marine and Fisheries Product
A. Berenike S. Diekmann Catriona Clemmesen Michael A. St. John Matthias Paulsen Myron A. Peck
Mar Biol (2012) 159:2399 2414 DOI 10.1007/s00227-012-1955-0 ORIGINAL PAPER Environmental cues and constraints affecting the seasonality of dominant calanoid copepods in brackish, coastal waters: a case
Effective desalting and concentration of in-gel digest samples with Vivapure C18 Micro spin columns prior to MALDI-TOF analysis.
Introduction The identification of proteins plays an important role in today s pharmaceutical and proteomics research. Commonly used methods for separating proteins from complex samples are 1D or 2D gels.
Copepod community growth rates in relation to body size, temperature, and food availability in the East China Sea: A test of Metabolic Theory
Copepod community growth rates in relation to body size, temperature, and food availability in the East China Sea: A test of Metabolic Theory Kuan-Yu Lin, Akash Sastri, Gwo-Ching Gong, and Chih-hao Hsieh
Announcements. Third problem site due November 30 Review calculations
Announcements Geology Primary Fields of Oceanography Chemistry Third problem site due November 30 Review calculations Next Thursday quiz on Kirchman reading (microbes) Moving from physical to biological