Date of birth: 19 August 1967 Marital status: married Children: 2 Sons Nationality: Dutch Place of birth: Assen, The Netherlands

Transcription

1 1. Curriculum Vitae Dr. Max Robert Gustaaf Roelfsema Personal Information Private address Neidertstraße Würzburg Germany Date of birth: 19 August 1967 Marital status: married Children: 2 Sons Nationality: Dutch Place of birth: Assen, The Netherlands Phone: Professional Information Work address Julius von Sachs Institute of Biosciences, Molecular Plant Physiology and Biophysics University of Würzburg Julius von Sachs Platz 2, Würzburg Phone: Fax: e mail: roelfsema@botanik.uni wuerzburg.de 1

2 Education High school Dr. Nassau College in Assen (The Netherlands) M.S. study biology at the Rijks universiteit Groningen (The Netherlands) 1992 M.S. degree in biology with specialization in plant physiology and molecular biology of plants at the Rijks universiteit Groningen (The Netherlands) PhD research with the topic Analysis of the signal transduction pathways of the stresshormone abscisic acid in guard cells with the use of abscisic acid insensitive abi mutants of Arabidopsis thaliana, supervised by Prof. Dr. Ir. P.J.C. Kuiper and Dr. H.B.A. Prins at the department of plant biology Rijks universiteit Groningen (The Netherlands) 1997 PhD degree in mathematics and natural sciences at the department of plant biology Rijks universiteit Groningen (The Netherlands) Professional experience Postdoctoral fellow at the department of plant physiology and biophysics at Wye College, University of London (Wye, Kent, UK) in the research group of Prof. Dr. M.R. Blatt, Project: Response of abi1 transformed Nicotiana bethamiana guard cells to carbon dioxide Postdoctoral fellow at the Department of Molecular Plant Physiology and Biophysics, University of Würzburg, Germany, in the research group of Prof. Dr. R. Hedrich. Project: Development of biophysical single cell techniques to study guard cells in intact plants. 2

3 Since 2003 Research assistant, at the Department of Molecular Plant Physiology and Biophysics, University of Würzburg, Germany, in the research group of Prof. Dr. R. Hedrich. Supervision of students, applying single cell techniques in combination with molecular biology, to study signal transduction pathways in leaf cells Qualification as university lecturer in Department of Molecular Plant Physiology and Biophysics, University of Würzburg, Germany. 3

4 2. Publications MRG Roelfsema Original papers 25. Stange A, Hedrich R, and Roelfsema MRG Ca 2+ dependent activation of guard cell anion channels, triggered by hyperpolarization, is promoted by prolonged depolarization. The Plant Journal 62: de Carbonnel M, Davis P, Roelfsema MRG, Inoue S, Schepens I, Lariguet P, Geisler M, Shimazaki KI, Hangarter R, and Fankhauser C The Arabidopsis PHYTOCHROME KINASE SUBSTRATE 2 protein is a phototropin signaling element that regulates leaf flattening and leaf positioning. Plant Physiology 152: Jeworutzki E, Roelfsema MRG, Anschütz U, Krol E, Elzenga JTM, Felix G, Boller T, Hedrich R, and Becker D Early signaling through the Arabidopsis pattern recognition receptors FLS2 and EFR involves Ca 2+ associated opening of plasma membrane anion channels. The Plant Journal 62: Marten H, Hyun T, Gomi K, Seo S, Hedrich R, and Roelfsema MRG Silencing of NtMPK4 impairs CO 2 induced stomatal closure, activation of anion channels and cytosolic Ca 2+ signals in Nicotiana tabacum guard cells. The Plant Journal 55: Engelmann JC, Deeken R, Müller T, Nimtz G, Roelfsema MRG and Hedrich R Is gene activity in plant cells affected by UMTS irradiation? A whole genome approach. Computational Biology and Chemistry: Advances and Applications, 2008: Levchenko, V, Guinot DR, Klein, M, Roelfsema MRG, Hedrich R and Dietrich, P Stringent control of cytoplasmic Ca 2+ in guard cells of intact plants compared to their counterparts in epidermal strips or guard cell protoplasts. Protoplasma 233: Krupenina NA, Bulychev AA, Roelfsema MRG and Schreiber U Action potential in Chara cells intensifies spatial patterns of photosynthetic electron flow and non photochemical quenching in parallel with inhibition of ph banding. Photochemical & Photobiological Sciences 7: Marten H, Hedrich, R and Roelfsema MRG 2007b. Blue light inhibits guard cell anion channels through a phototropin dependent pathway. The Plant Journal 50: Sano t, Becker D, Ivashikina N, Wegner LH, Zimmermann U, Roelfsema MRG, Nagata T and Hedrich, R Plant cells have to gain a threshold K + level to re enter cell cycle. The Plant Journal 50: Marten H, Konrad K, Dietrich, P, Roelfsema MRG and Hedrich R. 2007a Ca 2+ dependent and independent ABA activation of plasma membrane anion channels in guard cells of Nicotiana tabacum. Plant Physiology 143: Roelfsema MRG, Konrad KR, Psaras G, Marten H and Hedrich R Guard cells in albino leaf patches do not respond to photosynthetic active radiation, but are sensitive blue light, CO 2 and ABA. Plant Cell and Environment 29: Levchenko V., Konrad KR, Dietrich P, Roelfsema MRG and Hedrich R Cytosolic ABA activates guard cell anion channels without preceding Ca 2+ signals. Proceedings of the National Academy of Sciences of the United States of America 102: Meyerhoff O, Müller K, Roelfsema MRG, Latz A, Lacombe B, Hedrich R, Dietrich P and Becker D AtGLR3.4, a Glutamate Receptor Channel like Gene is sensitive to Touch and Cold. Planta, 222:

5 12. Roelfsema MRG, Levchenko V and Hedrich R ABA depolarizes guard cells in intact plants, through a transient activation of R and S type anion channels. The Plant Journal, 37: Becker D, Geiger D, Dunkel M, Roller A, Bertl A, Latz A, Carpaneto A, Dietrich P, Roelfsema MRG, Voelker C, Schmidt D, Mueller Roeber B, Czempinski K, Hedrich R AtTPK4, an Arabidopsis tandem pore K + channel, poised to control the pollen membrane voltage in a phand Ca 2+ dependent manner. Proceedings of the National Academy of Sciences of the United States of America 101: Becker D, Hoth S, Ache P, Wenkel S, Roelfsema MRG, Meyerhoff O, Hartung W and Hedrich R Regulation of the ABA sensitive Arabidopsis potassium channel gene GORK in response to water stress. FEBS Letters 554: Roelfsema MRG, Hanstein S, Felle HH and Hedrich R CO 2 provides an intermediate link in the red light response of guard cells. The Plant Journal 32: Szyroki A, Ivashikina N, Dietrich P, Roelfsema MRG, Ache P, Reintanz B, Deeken R, Godde M, Felle HH, Steinmeyer R, Palme K and Hedrich R KAT1 is not essential for stomatal opening. Proceedings of the National Academy of Sciences of the United States of America 98: Roelfsema MRG, Steinmeyer R, Staal M. and Hedrich R. 2001b. Single guard cell recordings in intact plants: light induced hyperpolarization of the plasma membrane. The Plant Journal 26: Roelfsema MRG, Steinmeyer R and Hedrich R. 2001a. Discontinuous single electrode voltageclamp measurements: assessment of clamp accuracy in Vicia faba guard cells. Journal of Experimental Botany 52: Ache P, Becker D, Ivashikina N, Dietrich P, Roelfsema MRG and Hedrich R GORK, a delayed outward rectifier expressed in guard cells of Arabidopsis thaliana, is a K + selective, K + sensing ion channel. FEBS Letters 486: Roelfsema MRG, Staal M. and Prins HBA Blue light induced apoplastic acidification of Arabidopsis thaliana guard cells: Inhibition by ABA is mediated through protein phosphatases. Physiologia Plantarum 103: Roelfsema MRG and Prins HBA The membrane potential of Arabidopsis thaliana guard cells; depolarizations induced by apoplastic acidification. Planta 205: Roelfsema MRG and Prins HBA Ion channels in guard cells of Arabidopsis thaliana (L.) Heynh. Planta 202: Roelfsema MRG and Prins HBA Effect of abscisic acid on stomatal opening in isolated epidermal strips of abi mutants of Arabidopsis thaliana. Physiologia Plantarum 95:

6 Reviews 6. Roelfsema MRG and Hedrich R Making sense out of Ca 2+ signals: their role in regulating stomatal movements. Plant Cell and Environment 33: Marten I, Deeken R, Hedrich R and Roelfsema MRG Light induced modification of plant plasma membrane ion transport. Plant Biology 12: Roelfsema MRG and Hedrich R Stomata. In: Encyclopedia of Life Sciences. Wiley and Sons Ltd. 3. Roelfsema, MRG and Hedrich, R In the light of stomatal opening new insights into "the Watergate". New Phytologist 167: Roelfsema MRG and Hedrich R Studying guard cells in the intact plant: Modulation of stomatal movement by apoplastic factors. New Phytologist 153: Hedrich R and Roelfsema MRG Plant ion transport. In: Encyclopedia of Life Sciences. Wiley and Sons Ltd. 6