NRL-Salmonella The Netherlands. Kirsten Mooijman
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1 NRL-Salmonella The Netherlands Kirsten Mooijman
2 Dutch NRL-Salmonella at RIVM/Z&O Situated at the Centre for Zoonoses and Environmental Microbiology (Z&O: approx. 60 staff members) Z&O is part of the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands (approx employees) 2
3 Organogram RIVM 3
4 Organogram Z&O Departments 4
5 Biological NRLs at RIVM-Z&O Salmonella (since ca 1993) Parasites (since ca 2005) Bacteriological and viral contaminants of bivalve molluscs (since ca 2005) Escherichia coli, including VTEC (since 2011) Listeria monocytogenes (since 2011) Coagulase pos Staphylococci, including Staphyloccocus aureus (since 2011) 5
6 Tasks and duties NRLs Defined in European legislation: For food and feed in: Regulation (EC) 882/2004 on official control performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules (April 2004) changed to Regulation (EU) 2017/625 (March 2017) 6
7 Tasks and duties of NRLs Collaborate with EURL in relevant area Coordinate activities of official national laboratories for analysis of samples Organise comparative tests between official national laboratories, including follow-up Disseminate information supplied by the EURLs to authorities and national laboratories Provide scientific and technical assistance to the national competent authority Quality system accredited according to ISO in relevant workfield 7
8 Collaborate with EURL in relevant area EURL and NRL for Salmonella situated at the same institute Important to separate activities for EURL and for NRL, especially for interlaboratory studies of the EURL. The technician who will participate in the interlaboratory study as NRL will receive blind samples, similar as for the other NRLs. 8
9 Coordinate activities of official national laboratories for analysis of samples NRL does not perform sample analysis for monitoring programmes; this is done by the official laboratories NRL gives advices/ visits/ trainings for official labs when needed NRL performs confirmation/ additional typing, e.g.: Occasionally analyse samples for second opinion (sero)typing of Salmonella isolates which official laboratories are not able to type to serovar level Confirmation of isolates typed as monophasic Salmonella Typhimurium by official laboratories 9
10 Organise comparative tests For analysis of Salmonella in food samples only one official national laboratory in NL: Netherlands Food and Consumer Product Safety Authority (NVWA). Both NRL and NVWA participate in PT (food) schemes of EURL. For analysis of Salmonella in samples from primary production stage (pps) and serotyping of isolates from these samples NL has 22 officially recognised (private) laboratories, of which 13 also perform serotyping of Salmonella. 10
11 Organisation comparative tests for pps Up to approx. 2009, NRL organised PT schemes itself (one study per year for detection of Salmonella pps). Since 2009 the ministry did not longer offered budget for organisation PT schemes for commercial (private) laboratories. Discussion of options with ministry and official laboratories (OLs): a) continue organising PTs by NRL and ask fee from OLs, or b) select a commercial PT scheme in which all OLs have to participate (for own costs) Agreed to go for option b) (less expensive than option a.). Labcodes of each OL have been forwarded to the NRL so that performance in PT schemes of each lab can be checked by NRL; NRL-Salmonella participates in the same PTs. 11
12 Comparative tests Prescribed PT schemes: VetQas of APHA, UK; accredited by UKAS: PT 0088: Salmonella in poultry PT 0084: Salmonella serotyping Each study: 4x 5 freeze dried samples per year Together with competent authority agreement on judgement of results of official laboratories: Per study, per lab: 5 samples okay: good; 4 okay: moderate; 3 okay: unsatisfactory Trend analysis of successive studies, per lab: Trend should (at least) be (over 4 studies): unsatisfactory moderate moderate good 12
13 Examples summarised results per PT scheme Samenvatting Salmonella detectie ringtest augustus 2016 Monsteroverzicht nummer pos / neg serotype (facultatief) en overige info 16/1628 +ve Salmonella Typhimurium (group B; > 1E5 cfu/vial) + Citrobacter youngae 16/1629 +ve Salmonella Enteritidis (group D; approx cfu/vial) + Pseudomonas aeruginosa 16/1630 -ve Citrobacter youngae 16/1631 +ve Salmonella Typhimurium monophasic (group B; approx cfu/vial) + E. coli 16/1632 +ve Salmonella Kedougou (group G; approx. 50 cfu/vial) Aug 2016 Code # correct opmerkingen Geen deelname /1628 en 16/1631 afgegeven als monofasisch S. Typhimurium ipv S. Typhimurium Monsters detectiestudie en typeringsstudie verwisseld. Resultaten in typeringsstudie voldoen aan resultaten detectiestudie, inclusief serotypering Geen deelname /1631 afgegeven als 'other' ipv S. Typhimurium Nil return Samenvatting Salmonella serotypering ringtest mei 2016 Monsteroverzicht nummer antigenen groep serovar 16/1370 1,4,[5],12:i:1,2 B S. Typhimurium 16/1371 1,6,14,25:y:e,n,x H S. Fischerkietz 16/1372 1,13,23:f,g,[s]:- G S. Havana 16/1373 1,4,[5],12:i:- B monofasisch S. Typhimurium 16/1374 1,13,23:i:l,w G S. Kedougou Mei 2016 Code # OK VetQas # OK NVWA Opmerkingen: Geen deelname Nil return, maar NRL geïnformeerd en ruwe data toegestuurd /1373 afgegeven als 'other', terwijl monofasisch S. Typhimurium 3 onderdeel is van erkende serotypes; 16/1374 onterecht afgegeven als monofasisch S. Typhimurium /1373 onterecht afgegeven als S. Gloucester /1373 niet volledig uit getypeerd (afgegeven als groep B), terwijl monofasisch S. Typhimurium onderdeel is van erkende serotypes Rood: Blauw: Geel: onvoldoende matig attendering Rood: Blauw: onvoldoende matig opmerking 13
14 Example summarised results, trends in PT schemes 14
15 Results comparative tests In case of unsatisfactory results in one study NRL contacts OL: explanation for poor results? Advices needed? In case of unsatisfactory trend results of several successive PTs NRL contacts OL and Competent Authority (CA). CA can decide to (temporary) suspend the recogniction of an official laboratory. Actions for improvement: e.g. training at NRL, audit by NRL 15
16 Disseminate information supplied by EURL to authorities and national laboratories EURL-Salmonella reports are forwarded to Competent Authority Newsletters EURL-Salmonella are also sent to Competent Authority Technical information from EURL- Salmonella forwarded to official laboratories 16
17 Assist the national competent authority Cooperate with Netherlands Food and Consumer Product Safety Authority (NVWA), e.g. for recognition of official laboratories Discuss/ give advice for diagnostics and monitoring in relation to national programmes Discuss/ give advice on new/amended EC legislations Participate in committees at national level Assist in case of outbreaks 17
18 Quality system accredited according to ISO in relevant workfield RIVM-Z&O accredited since 2005; annual visit by the Dutch Accreditation Council Since 2005 scope has been enlarged until currently 12 activities For NRL-Salmonella (and EURL): detection of Salmonella in food, animal feed and pps conform EN ISO 6579:2002 and Annex D: Next year conform EN ISO Registration under: L421 18
19 Thank you for your attention! Questions? RIVM 19
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