colorphlex Chatsworth, CA Anti-breakage Benefits of Hair Care Formulations

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1 clrphlex Chatswrth, CA Anti-breakage Benefits f Hair Care Frmulatins Objective T evaluate the anti-breakage prperties in the dry state fr tresses treated with varius test prducts. Prducts The fllwing prducts were prvided by the requester fr use in the anti-breakage study: Clr additive - Test prduct #1 (Lt# BL A) Finishing Cnditiner -Test prduct #2 (BL B) Experimental Hair The hair tresses weighed apprximately 3 g and measured 8 in length and 1 in width. Prir t testing, the tresses were bleached fr Cells 2, 3, & 4. Treatment All prduct treatments were in accrdance with infrmatin prvided by the requestr. Grups f tresses were treated with ne f the regimens utlined belw. All the swatches were treated as per standard prduct applicatin Cell 1 (Virgin Hair) Washed with 0.3 ml SLES/tress (massaged and rinsed fr 30 secnds) Cell 2 (9% Bleached Hair) After bleaching, tresses were standardized by washing with 0.3 ml SLES/tress (massaged and rinsed fr 30 secnds) Cell 3 (9% Bleached Hair + Clr additive) Clr additive (3.75 ml/unce f bleach) was added t the bleach mixture then standardized with 0.3 ml SLES/tress (massaged and rinsed fr 30 secnds) Cell 4 (9% Bleached Hair + Clr additive + Finishing Cnditiner) Clr additive (3.75 ml/unce f bleach) added t the bleach mixture then standardized with 0.3 ml SLES/tress (massaged and rinsed fr 30 secnds) treated with finishing cnditiner (15% -0.45ml/tress) (leave n fr 10 minutes and rinsed fr 30 secnds)

2 Anti-Breakage by Repeated Grming A useful methd t determine whether a treatment will prtect hair against breakage is prvided by repeated grming experiments; in this test, the number f brken fibers is recrded as a functin f repeated cmbing/brushing strkes. Any treatment that reduces snags, entanglements and abrasin can help in substantially lwering the number f brken fibers. A custm-built autmated grming device was used cnsisting f a hllw rtating drum-like assembly, where fur uter crssbars cntain hlders fr munting cmbs r brushes. These uter arms are detachable t allw fr different hlders t be munted and experiments t be perfrmed using a variety f cmbs r brushes. One cmplete drum revlutin cmbs (r brush) a tress fur times. This entire set-up is duplicated three times in the hrizntal directin allwing fur tresses t be cmbed simultaneusly. Cllectin plates are lcated under each tress t save brken fiber fragments, while spacer plates n the rtating drum prevent crss cntaminatin. All experiments were again perfrmed after vernight equilibratin f the hair at 60% RH. were repeatedly brushed 10,000 times with brken fibers being evaluated every 1000 strkes. Eight replicate hair tresses were used fr each sample t ensure statistical relevance. This methdlgy has als been dcumented in the scientific literature (Evans & Park, A Statistical Analysis f Hair Breakage. II. Repeated Grming Experiments, J.Csmet.Sci., 61, , 2010).

3 Repeated Grming Results Bx and whisker plt was generated using Statistica, while JMP analytical sftware was used t calculate the statistics (student s t-test at 95% cnfidence level). Raw data is given in the Appendix at the end f this reprt. Result frm repeated grming testing after 10,000 strkes is shwn belw. 140 Bx Plt f Number f brken f ibers af ter 10,000 strkes gruped by Treatment Earthly Bdy Anti-Breakage Number f brken fibers after 10,000 strkes Cell 2 Cell 3 Cell 1 Cell 4 Treatment Mean Mean±SE Mean±SD Outliers Extremes Results fr Anti-Breakage Number Treatment Mean Std Dev Std Err Mean Cell 2 (9% Bleached hair) A Cell 3 (9% bleached hair + Clr Additive) B Cell 1 (Virgin Hair) C Cell 4 (9% bleached hair + Clr Additive + Finishing cnditiner) C Levels nt cnnected by same letter are significantly different.

4 % Reductin in breakage is calculated as belw, Mean# Brken Fibers f Treatment % Reductin in Breakage = (1 ) 100 Mean# Brken Fibers f Cntrl Results shw that Cell 2 (9% bleached hair) presents the highest level f breakage as cmpared t the ther cells. Cell 3 (9% bleached hair + Clr Additive) shws a reductin in breakage statistically different frm Cell 2 (9% bleached hair). Ntably, Cell 4 (9% bleached hair + Clr Additive + Finishing cnditiner) shws the highest reductin in breakage ranking statistically equivalent t Cell 1 (Virgin hair).

5 Raw Data Brush Strkes Cell 1 (Virgin Hair) Ttal Brush Strkes Cell 2 (9% Bleached Hair) Ttal

6 Brush Strkes Cell 3 (9% Bleached Hair + Clr Additive) Ttal Brush Strkes Cell 4 (9% Bleached Hair +Clr Additive + Finishing Cnditiner) Ttal