Rare donor program in Japan
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1 ISBT Working Party on Rare Donors Rare donor program in Japan Yoshihiko Tani, MD, PhD
2 Have a national Rare Donor Program
3 Rare Blood in Japan Category I (35) Bombay para-bombay M k /M k En(a ) MiV/MiV N sat /N sat S /s /U p P k Rhnull Rhmod D cd LW(a b ) Lu(a b ) In(Lu) Ko Kp(a+b ) Kp(a b ) K1 k Kmod McLeod Fy(a b ) Jk(a b ) I Ge: 2, 3 Lan IFC UMC Dr(a ) Gy(a ) Ok(a ) JMH Er(a ) Category II (5) s Fy(a b+) Di(a+b ) Jr(a ) Do(a+b )
4 Number of Donors in Database
5 Registration of Rare Donors in Japan (Category I) as of Rare Blood RhD + RhD Bombay 5 0 M k M k 1 0 En(a ) 2 0 MiV / MiV 0 Nsat/Nsat 1 0 S s U 2 1 P k 2 0 Rhnull - 5 Rhmod - 7 Kp(a b ) 2 0 k 8 0 K: Fy(a b ) 6 0 Rare Blood RhD + RhD McLeod 19 0 I 19 0 Ge 11 0 Lan 16 0 Gy(a ) 6 0 Ok(a ) 19 0 JMH 12 0 para- Bombay 77 0 p In(Lu) 93 1 Ko Jk(a b ) 69 1
6 Registration of Rare Donors in Japan (Category Ⅱ) Category I Rare Blood RhD + RhD Fy(a ) 3, Di(b ) 2, Jr(a ) 2,130 2 s as of
7 Number of Rare Blood Donors registered (Category I) ( )
8 Number of Rare Blood Donors registered (Category I) ( )
9 Number of Rare Blood Donors registered (Category II) ( )
10 Number of Frozen Rare Cells
11 Number of Frozen Rare Cells Rare Blood A O B AB Bombay 18 para-bombay Rhnull 13 Rhmod p P k 3 En(a ) 8 15 MiV/MiV 7 Ko McLeod Jk(a b ) I Ge Lan Gy(a ), Hy 6 1 Ok(a ) Fy(a b ) In(Lu) as of numbers
12 How are Rare Donors found?
13 Monoclonal antibodies for blood grouping (1) Cell line MoAb Cell line MoAb Cell line MoAb OSK1 OSK7 anti-p k OSK15-1 anti-cd OSK1-1 OSK8 anti-u+n OSK15-2 anti-p OSK2 OSK9 OSK16 anti-rh29 anti-a anti-h OSK2-1 OSK9-1 OSK16-1 (anti-rh17) OSK2-2 OSK10 OSK17 OSK3 OSK10-1 anti-b OSK17-1 OSK3-1 OSK10-2 OSK17-2 anti-p1 OSK3-2 anti-d OSK11 anti-m OSK17-3 OSK3-3 OSK12 OSK18 anti-le related anti-n OSK3- OSK12-1 OSK18-1 anti-le bh OSK anti-en a FR OSK13 OSK19 anti-fy6 OSK-1 anti-en a FS OSK13-1 anti-le a OSK20 OSK-2 anti-en a TS OSK13-2 OSK20-1 anti-e OSK5 anti-k2 OSK1 anti-i OSK20-2 OSK6 anti-ge OSK15 anti-p OSK21 anti-acquired B red:human MAb :rare blood screening
14 Monoclonal antibodies for blood grouping (2) Cell line MoAb Cell line MoAb Cell line MoAb OSK22 OSK29-2 OSK36 anti-kp b OSK22-1 anti-fy3 OSK29-3 OSK37 anti-di a OSK22-2 OSK29- anti- OSK38 anti-xg a OSK23 anti-t OSK29-5 GPA&B OSK39 anti-ifc OSK2 OSK29-6 OSK0 anti-miltenberger anti-tn OSK2-1 OSK29-7 OSK1 anti-fy b OSK25 OSK29-8 anti-gpa OSK2 anti-e anti-k1 OSK25-1 OSK30 anti-jr a OSK3 anti-lan OSK26 anti-c OSK31 anti-di b OSK anti-hro OSK27 anti-tk OSK32 anti-k5 (Ku) OSK6 anti-kg OSK28 anti-i OSK33 anti-kp c OSK7 anti-fy a OSK29 OSK3 anti-k1 OSK8 anti-c anti-gpa&b OSK29-1 OSK35 anti-jk b OSK9 anti-s red:human MAb :rare blood screening OSK-MoAb: 9 kinds, 93 cell lines
15 Blood Grouping Reagent : Cross-linked Monoclonal antibody mix Human MoAb Mouse anti-human IgG MoAb Red Blood cells Cross-linked MoAb agglutination
16 Total volume of monoclonal antibodies supplied to other blood centers( ) Year No. of MoAbs No. of Blood centers supplied Total volume (ml) ,890 18,290 15,50 16,80 19,85 20,60 18,05 30,38 50,301 59,018 9,187 32,217 28,655 39,731 56,962 75,770 77,005 87, ,910 97, , , , ,20 55, ~: anti-en a, anti-hro(rh17) anti-k2, anti-ge anti-u+n, anti-cd 1991~: anti- I 199~: anti-jr a, anti-di b 2005~: anti-lan 2007~: Consolidating of laboratory Screening of rare blood Screening of antigen negative blood Blood typing
17 Automated machine PK7300 Japanese Red Cross OSAKA Blood Center
18 Images of hemagglutination on PK7300 D ABO antibody rare blood detamination A D Ab A D+ Ab A D+ Ab O D+ Ab O D+ Ab O D+ Ab B D+ Ab B D+ Ab 9 10 AB D+ AB D+ Ab Ab
19 Number of Rare Donor units used per year
20 Units of Rare Blood Provided (Category I) ( )
21 Units of Rare Blood Provided (Category I) ( ) units Jk(a b ) p Ko Bombay (para)
22 Units of Rare Blood Provided (Category II) ( ) Units Jr(a ) Fy(a ) Di(b )
23 Units of Rare Blood Internationally shipped ( ) year country rare blood units 1977 Germany(W) Di(b ) Germany(W) Di(b ) USA Germany(W) Germany(W) Germany(W) France Thailand Germany(W) Thailand Germany(W) 1986 Germany(W) Di(b ) Di(b ), Ccee, Fy(b-) Di(b ), Jk(b-) Bombay(Para-Bombay) Di(b ), Jk(b-) p Di(b ), Jk(b-) Di(b ), Jk(b-) Di(b ) Ko 1987 Germany(W) Ko year country rare blood units USA Germany(W) Thailand USA Germany(W) Thailand MkMk Ko Bombay Jr(a ) Ko Di(b ) 1991 Thailand Bombay Australia Thailand Di(b ) Ko 1996 Sweden Di(b ) Taiwan USA Switzerland Norway Argentina UK Jk(a b ) Ko Jr(a ) Jr(a )
24 Units of Rare Blood Internationally shipped ( ) year country rare blood units Sweden USA Netherlands Indonesia UK China Canada En(a ) Jr(a ) Ko Para-Bombay Di(b ) 2002 Philippine Jk(a b ) Spain Philippine USA Korea Brazil Brazil Thailand Czech Republic Philippine Jk(a b ) Jr(a ) Ko, D p Jk(a b ) year country rare blood units 2006 Thailand China Ko Fy(a ), C 2007 New Zealand Netherlands Thailand Netherlands Thailand Malaysia UK Australia Canada UK New Zealand Di(b ), D Fy(a-),C-,e-,Jk(b-) Di(b ), D Fy(a-),C-,e-,Jk(b-) p, Fy(b ) p Ko 2012 Australia Lan
25 Units of Rare Blood Internationally shipped 1 ( ) country year rare blood units Germany (West) Di(b ) Di(b ) Di(b ) Di(b ), Fy(b-),Ccee Di(b ), Jk(b-) Di(b ), Jk(b-) Di(b ), Jk(b-) Di(b ), Jk(b-) Di(b ) Ko Ko Ko Ko France Norway 1998 Jr(a ) 8 Sweden Di(b ), C, S, P 2 En(a ) 8 12 country year rare blood units Netherlands Ko Di(b ), D Switzerland 1998 Ko, c E,Fy(b-) 6 UK Jr(a ) Spain Czech Republic USA Canada M k M k Jr(a ) Jr(a ) Jr(a ), Fy(b ), Jk(a ) Ko
26 Units of Rare Blood Internationally shipped 2 ( ) country year rare blood units country year rare blood units Thailand Philippine Bombay(Para-Bombay) p Bombay Di(b ) Bombay Ko Jk(a b ) p p Ko, Le(a ) Fy(a ), C e, Jk(b ) Jk(a b ), c E Jk(a b ) Taiwan 1997 Jk(a b ) 11 Hong kong 1997 Di(b ) 8 China Di(b ), Jk(a ) Fy(a ), C Korea Australia New Zealand Di(b ) p Lan Argentina Indonesia 2000 Para-Bombay 10 Malaysia 2009 p, Fy(b ) Brazil 200 Ko, D 6
27 Countries & Rare Blood Internationally shipped ( ) 23 countries Germany, Sweden, Norway, France, Netherlands, UK, Switzerland, Spain, Czech Republic, USA, Canada, Argentina, Brazil, Thailand, Philippine, China, Taiwan, Hong Kong, Korea, Indonesia, Malaysia, Australia, New Zealand rare blood units numbers rare blood units numbers Di(b ) Bombay (para-) p 22 5 Jr(a-) 72 6 En(a ) 12 1 Ko M k M k 6 3 Jk(a b ) 7 10 Lan 2 Fy(a ) 28 Total
28 Japanese Red Cross OSAKA Blood Center
29
30 Japanese Red Cross OSAKA Blood Center
31 Screening for rare blood by monoclonal antibodies (1987 ~ 2011) Monoclonal Antibody No. of tested Rare Blood No. of detected Frequemcy anti-u+n 2,062,873 U % anti-en a 12, anti-hro 25,703,06 En(a ) % M k M k % % Rhnull % anti-cd 6,850,028 Lu(a b ) % anti-k2, anti-k5, anti-k1 17,265,220 Including repeat donors Ko % McLeod or Kmod % anti-ge 7,828,135 Ge % anti-lan 7,197,279 Lan % anti-fy3 795,239 Fy(a b ) 0 0 % anti-ifc 610,271 IFC % anti-i 1,138,211 I 0 0 % anti-jr a 21,161,01 Jr(a ) 11, % anti-di b 2,532,761 Di(a+b ), %
32 Frequency of Rare Blood (Japan) 1/100 1/1,000 Fy(a ) Di(b ), s Jr(a ) Summarized by Dr. Uchikawa in Tokyo BC 1/10,000 1/100,000 1/1,000,000 Lu(a b ), InLu Ko, Lan Jk(a b ), Ok(a ) Ge D Rhnull Gy(a ), Kx (McLeod) I Oh P Fy(a b ) En(a ) En a FR (MiV/MiV, N SAT /N SAT ) IFC Dr(a )
33 Other items of interest
34 Rare Blood Internationally Provided to Japan rare blood country units 197 D Canada 1977 p USA Canada I USA En(a ) UK Ge Sweden USA
35 Thank you for your attention. 38
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