A Study on the Purification of the Flavonoids from Aloe Leaf Powder with Macroporous Resin
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1 Acta Agriculturae Universitatis Jiangxiensis http / /xuebao. jxau. edu. cn E - mail ndxb7775@ sina. com H1020 ph 3. 0 φ = 80% 12 mg /ml φ = 80% 2. 0 ml /min S Q A A Study on the Purification of the Flavonoids from Aloe Leaf Powder with Macroporous Resin YANG Wu-ying SHANGGUAN Xin-chen WU Lei-yan YANG Yong LI Hong-min JIANG Xing College of Food Science and Engineering Plant Resource Exploitation Utilization Reseach Room JAU Nanchang China Abstract The purification of the flavonoids from aloe leaf powder with macroporous resin was studied. The static adsorption rate and desorption rate of five different macroporous resins were surveyed under the same conditions to select the best one. The optimum purificated technic was confirmed by studying the static and dynamic absorption and desorption of flavonoids with the best resin under different conditions. The result indicated that the optimum macroporous resin was H1020 and its static adsorptive ability was the strongest at room temperature and ph = 3 the most effective desorption could be obtained with 80% volume fraction alcohol. The best dynamic absorption was obtained at room temperature and crude flavonoids solution at 12 mg /ml the most effective desorption was also obtained with 80% volume fraction alcohol at 2. 0 ml / min desorption rate. Key words Aloe flavonoids macroporous resin purificaon P GJJ E - mail yangwuyi2003@ yahoo. com. cn
2 H1020 D301 AB - 8 X - 5 NKA HF - 20B 754pc mg φ = 80% 25 ml ml 10 ml ρ = 5% 0. 3 ml 6 min ρ = 10% 0. 3 ml 6 min ρ = 4% 4 ml 15 min 754PC ml A V = 100% M 1 V /ml M /g kg HF - 20B φ = 80% W 1 20 min NaNO 2 - Al NO % h g ml 50 ml r /min 24 h φ = 60% 25 ml r /min 24 h Q a = C o - C r V a /W α /% = C o - C r / C o Q d = C d V d /W β /% = C d V d / C o - C r V a α % Q a mg /g C o mg /ml C r mg /ml V α ml W g Q d mg /g C d mg /ml V d ml β %
3 1 171 Tab Physical characters of 5 kinds of macroporous resin /mm / m 2 g - 1 /A / ml g - 1 Resin Polar Size range Specific surface area Average pore size Pore volume NKA ~ ~ ~ ~ AB ~ ~ ~ ~ D ~ H ~ ~ ~ ~ X ~ ~ ~ ~ g r /min ml ρ =5% 0. 3 ml ph g ph r /min 0. 6 ml ρ = 5% 0. 3 ml g 20 φ = 0% 20% 40% 60% 80% 100% 30 ml ml ρ = 5% 0. 3 ml cm 10 cm 2. 0 ml /min mg /ml 2 10 cm φ = 40% 60% 80% 2. 0 ml /min 2 min Fig. 1 The standard curve of rutin 754PC 510 nm C = A R 2 =
4 Tab Comparison of static adsorption of 5 kinds of macroporous resin to total flavonoids in aloe leaf powder Macroporous resin /% Adsorption rate / mg g - 1 Adsorptionamount /% Desorptionrate / mg g - 1 Desorptionamount H D AB X NKA H1020 AB - 8 H1020 X - 5H H1020 Fig. 2 Effects of temperature on H1020 H1020 macroporous resin adsorption rate H H H H H ph Tab. 3 Effects of ph value on adsorption rate ph /% Absorption rate ph H ph H ph = ph = ph = 3 ph H
5 1 173 φ = 80% 4 H % 40% Tab. 4 Effect of different concentration ethanol on static desorption of H1020 macroporous resin φ = 40% /% 60% 80% Ethanol volume fraction / mg g - 1 Desorption amount H H Tab. 5 Effect of concentration of mass crude flavone extracted from Aloe leaf powder solution on the dynamicabsorption of macroporous resin / mg ml - 1 Feed liquid mass concentration 5 /ml Weep point 12 mg /ml 12 mg /ml H φ = 80% 35 φ = 40% 30 φ = 60% 35 φ = 80% H % 60% 80% Fig. 3 Dynamic desorption curves of macroporous resin with mass fraction of 40% 60% and 80% ethanol H1020 ph 3 φ = 80% 12 mg /ml 2. 0 ml /min φ = 80% H1020 NaNO 2 - Al NO 3 3 H % 35% 30% 20%
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