808 nm. Optical Transport Characteristics of Human Tissues in Vitro at 808 nm Linearly Polarized Laser Irradiation

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1 CHIN ESE J OURNAL OF LASERS Vol. 3 No. 3 March 2004 : (2004) nm 3 2 ( 5063 ; ) 808 nm Kubelka2Munk 808 nm ( P < 00) 808 nm 808nm ( P > 005) 808 nm 808 nm ( P < 00) 808 nm Kubelka2Munk 808 nm ; ; ; ;808 nm ; R A Optical Transport Characteristics of Human Tissues in Vitro at 808 nm Linearly Polarized Laser Irradiation WEI Hua2jiang XIN G Da WU Guo2yong 2 GU Huai2min J IN Ying Institute of L aser L if e Science South China Norm al U niversity Guangz hou Guangdong 5063 China 2 First A f f iliated Hospital of S un Yat2sen U niversity of Medical Sciences Guangz hou Guangdong China Abstract In this paper a double2integrating2spheres system basic principle of measuring technology of ray radiation optical model of biological tissues were used to study the optical properties of human tissues. The results of measurement showed that the parameters of optical properties of human normal bladder and human bladder cancer tissue at 808 nm laser and the linearly polarized laser in Kubelka2Munk two2flux model had prominent distinction ( P < 0. 0). The parameters of optical properties of human normal bladder tissue for 808 nm laser and the linearly polarized laser had all distinction. Absorption coefficient and effective attenuation coefficient among all parameters of optical properties of human normal bladder tissue had obviously distinction. All parameters of optical properties of human bladder cancer tissue for 808 nm laser and the linearly polarized laser had not prominent distinction ( P > 0. 05) which showed that the depolarization of human bladder cancer tissue to 808 nm linearly polarized irradiation was very big. The parameters of optical properties of human normal small intestine tissue at 808 nm laser and the linearly polarized laser irradiation had all prominent distinction ( P < 0. 0) which showed that the depolarization of human normal small intestine tissue to 808 nm linearly polarized irradiation was smaller. Distribution of light intensity of three kinds of human tissues at 808 nm laser and the linearly polarized laser irradiation in Kubelka2Munk two2flux model had obviously dissimilitude. Key words laser technique ; optical properties ; small intestine ; bladder ; cancer tissues ; 808 nm laser ; double2 integrating2sphere system : ; : : (2002CCC00400) (0502) : (96 ) E2mail com 3 E2mail edu. cn

2 306 3 R a = 6 mm R b = 2 mm [ ] : ;2 : ;3 : R a = 6 mm ;4 : R b = 2 mm Fig. Map of the exhibition of the tissue2sample holder : tissue2sample ; 2 : tissue2sample holder ; [2 ] ( nm) 808 nm ( PD T) 2 [3 ] Fig. 2 Experimental setup of double2integrating2sphere system for measuring the optical properties of biological tissues 808 nm mm mm 3 : R a = 6 mm ; 4 : R b = 2 mm : ;2 3 : ;4 : ;5 8 : ;6 : ;7 : ;9 2 : ;0 : ; 3 : ;4 5 : ;6 7 : : laser ; 2 3 : attenuator ; 4 : mirror ; 5 8 : aperture ; 6 : beam expander ; 7 : polarizer ; 9 2 : intergrating sphere ; 0 : sample ; 3 : optical trap ; 4 5 : detector system ; 6 7 : baffle : ( ) 808 nm 4 h 25 6 mm 64 mm mm mm 85 mm 32 2 mm

3 3 : 808 nm 307 T c 2 mm 25 = ( I s / I o ) - T d (4) 6 mm I s I o 2. 3 ( X SD) SPSS0 for Windows t 808 nm [4 ] : R [5 ] d R d = K s / K p Kubelka2Munk () Kubelka2Munk K p K s Kubelka2Munk A KM S KM T d R T d T d = I s / I o (2) [6 ] I s I o X (cm) 3 3 i ( x) j ( x) I ( x) [7 ] A KM S KM [8 ] 3 6 : ;2 5 : ;3 4 : ;7 : = 3 E t = A KM + S KM (7) Fig. 3 Measurement method of specular reflectance and collimated transmittance of biological tissue 6 : intergrating sphere ; 2 5 : sample ; 3 4 : baffle ; 7 : standard reference plate= A KM S KM E t E eff A KM 3 S KM 3 E 3 t nm 0 R m EXCEL for Windows R m = K s / K p - R d (3) 808 nm K p K s T c 3 [4 ] A KM S KM A KM = [ ( + R 2 + T 2 ) / 2 R - ] S KM (5) S KM = - R/ ( a + b) ln Xb T E t E eff (6) E eff = A KM ( A KM + 2 S KM ) (8) X Eeff 3 4

4 308 3 ( P > 005) R d T d R m T c R 3 d T 3 d R 3 m T 3 c 4. Kubelka2Munk Kubelka2Munk 808 nm Kubelka2Munk (5) 808 nm (8) 2 Kubelka2Munk 808 nm Table Diffuse reflectance diffuse transmittance specular reflectance collimated transmittance of human tissues in Kubelka2Munk two2flux model at laser and linearly polarized laser irradiation Tissues R d T d R m T c R 3 d T 3 d R 3 m T 3 c Normal small intestine Normal bladder Bladder cancer Kubelka2Munk 808 nm Table 2 Absorption coeff icients scattering coeff icients total attenuation coeff icients effective attenuation coeff icients of human tissues in Kubelka2Munk two2flux model at laser and linearly polarized laser irradiation Tissues A KM / cm - S KM / cm - E t / cm - E eff / cm - A 3 KM / cm - S 3 KM / cm - E 3 t / cm - E 3 eff / cm - Normal small intestine Normal bladder Bladder cancer Fig nm Forward scattered photon fluxes backward scattered photon fluxes total scattered photon fluxes of human normal small intestine human normal bladder human bladder cancer tissues in Kubelka2Munk two2flux model at 808 nm laser and linearly polarized laser irradiation 4. 2 Kubelka2Munk Mathcad200 for Windows 4 A ( x) Kubelka2Munk B ( x) 808 nm 808 nm i ( x) j ( x) C ( x) D ( x) 808 nm I ( x) E ( x) F( x) nm

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