Two-photon single-beam particle trapping of active micro-spheres
|
|
- Victoria Hodge
- 5 years ago
- Views:
Transcription
1 Two-photon single-beam particle trapping of active micro-spheres Dru Morrish, Xiaosong Gan and Min Gu * Centre for Mirco-Photonics, School of Biophysical Sciences and Electrical Engineering, Swinburne University of Technology, PO Box 218, Hawthorn, VIC 3122, Australia. ABSTRACT In this paper, we have demonstrated the measurement of the morphology-dependent resonance 1 (MDR) within a laser trapped microsphere excited under two-photon excitation. Both trapping and two-photon excitation are achieved by a single femtosecond pulsed laser beam simultaneously. MDR induced by two-photon excitation 2 has also shown to overcome difficulties in separation of excitation and resonant wavelengths and the inability to confine excitation illumination precisely 3. A spectrally resolved image demonstrates that this technique provides an alternative imaging mechanism for near-field imaging and mapping surface tomography. Keywords: Two-photon excitation, micro-cavity, morphology dependant resonance, laser trapping. 1. INTRODUCTION Laser trapping has led to a strong impact on biological studies and chemical scientific research at a single molecule level 4-6 as well as scanning near-field optical microscopy (SNOM) 7,8. Laser trapping SNOM utilizes a trapped micro-particle as a scanning probe for near-field imaging and has some advantages over conventional SNOM which uses a tapered fiber tip as a scanning probe. One of the most significant advantages is that controlling the distance between the probe and substrate is not required in laser trapping SNOM 7,8. In addition, the concern associated with a fragile probe is not an issue in laser trapping SNOM. However, due to the low signal strength, image contrast in laser trapping SNOM requires a significant improvement. One solution to this problem is the utilization of morphology-dependent resonance (MDR) 1,9-12 in a trapped micro-sphere. A dielectric sphere possesses natural internal modes of oscillation at characteristic frequencies corresponding to specific ratios of size to wavelength, which are called MDR This feature provides a useful tool for enhancing the signal strength in sensing and imaging with a trapped micro-sphere 12. MDR can be induced in fluorescent micro-spheres under single-photon 1,12 and two-photon 3 excitation. To induce MDR in a trapped micro-sphere, one normally needs two beams; one is for trapping a micro-sphere and the other for fluorescence excitation. It is, however, difficult to dynamically control the two focal spots with high accuracy. To overcome such a difficulty, we introduce a novel technique in which case both trapping and two-photon excitation are achieved by a single femtosecond pulsed laser beam simultaneously. 2. EXPERIMENTAL RESULTS The effect of a femtosecond pulsed laser beam on laser trapping performance was demonstrated in the experimental system shown in Fig. 1. A train of linearly polarized 86 fs pulses of wavelength 870 nm (Spectra-Physics Tsunami) is coupled directly into an inverted trapping microscope objective so that the back aperture of the trapping objective is filled. A high numerical aperture (NA=1.2) water immersion objective (Olympus UplanXW60) is used to focus the pulsed laser beam into a sample cell. The sample cell consists of Yellow-Green fluorescent micro-spheres of 10 µm in diameter (Polysciences), which has an absorption peak close to the laser wavelength for two-photon excitation 12. The micro-spheres are suspended in water within a sealed sample cell. The displacement of a trapped * Phone: , mgu@swin.edu.au Optical Trapping and Optical Micromanipulation, edited by Kishan Dholakia, Gabriel C. Spalding, Proceedings of SPIE Vol (SPIE, Bellingham, WA, 2004) X/04/$15 doi: /
2 particle is achieved by a computer-controlled scanning stage on which the sample cell is attached. Throughout this paper we employ an s-polarized trapping beam, meaning that the polarization direction of a trapping beam perpendicular to the direction of the transverse displacement of a trapped particle. The fluorescence emission from an excited micro-sphere, which exhibits the MDR feature 3, is analyzed by a high-resolution spectrograph (ARC, λ= nm). Photo-polymerized grating structure attached to coverslip* Sample cell Olympus H 2 O immersion (NA=1.2) Tsunami 86 fs 82 MHz = 870 nm Mechanical shutter* Scanning Stage Flip Mirror CCD YG micro-spheres 10 m diameter in water Spectrograph Figure 1. Schematic of laser trapping experimental setup. Items marked with a * inserted for two-photon photo-polymerisation grating fabrication. The strength of the two-photon-induced MDR is highly dependent on the location of the excitation spot within a micro-sphere because of the highly localized nature of two-photon absorption (Fig.2). Therefore, the strength and the visibility of the MDR signal varies with translation velocity of a trapped particle as a result of the balance between the viscous drag force and the transverse trapping force. In order to quantify the MDR feature in relation to the translation velocity of a trapped particle, we introduce the measurable quantity, the visibility V defined as V = ( I peak I background ) /( I peak + I background ), where I peak and I background are the intensity of MDR peaks and the background fluorescence, respectively 3. The MDR signal induced in a stable laser trapped particle for various translation velocities is shown in Fig. 3(a-f). It is shown that the MDR effect is greatly enhanced when the translation velocity increases. This phenomenon can be explained as follows. At a given laser power, the greater the translation velocity, the greater the transverse trapping force required. This means that the trapping spot moves towards the edge of a trapped particle because the transverse trapping force increases with the displacement of the trapping beam 13. It has been previously demonstrated that the two-photon-induced MDR effect becomes more significant when the excitation spot moves closer towards the edge of a trapped particle (Fig. 2.). Therefore, the visibility of the MDR signal becomes pronounced when a particle is scanned fast. This feature implies that a better sensitivity in laser tapping SNOM could be achieved at a high scanning velocity. 604 Proc. of SPIE Vol. 5514
3 Figure 2. The visibility of a MDR peak as a function of localized excitation spots in the radial direction of the equatorial plane. It should be pointed out that the induced MDR signal is highly polarized 3. The two adjacent peaks in the MDR fluorescence spectrum represent two cavity modes, the transverse electric (TE) and transverse magnetic (TM) modes. It is shown in Fig. 3(g). that both modes share a similar increase in visibility with increasing translation velocity. Visibility (e) nm (g) nm (a) (c) Velocity (µm/s) (b) (d) (f) Figure 3. MDR spectra of a laser trapped micro-sphere at velocities 4, 9, 14, 19, 26 and 29 µm/s ((a) to (f)), respectively. Visibility of peaks nm (squares) and nm (circles) as a function of the translation velocity of a trapped particle (g). Proc. of SPIE Vol
4 In order to characterize the imaging properties of a laser trapped probe, a polymer test sample was fabricated. The test sample consists of a grating structure fabricated using a two-photon photo-polymerization technique14. The grating has a lateral spacing of 10 µm, a full width half maximum (FWHM) of 0.7 µm and is 600 nm in height. The spectral resolved one-dimensional (1-D) image of the grating structure is shown in Fig. 4. The spatial resolution of the image is 1 µm/point according to the scanning speed (10 µm/s ) and the spectrograph CCD cameras exposure time (100 ms). It is noted in Fig 4. that the MDR effect is enhanced at the position of each element in the grating. This is because when the scanned particle collides with an element of grating structure the trapping spot is pushed further toward the edge of the particle, resulting in stronger MDR signal sity Inten ) (a.u W av ele ) ng th 10 x µm ( 540 (n 550 m ) Figure 4. One-dimensional spectral image of photo-polymerized grating structure. 3. CONCLUSION In conclusion, we have demonstrated the achievement of simultaneous trapping and two-photon induced MDR in a micro-sphere by a single ultrashort-pulsed beam. The use of a femtosecond-pulsed beam allows for localized twophoton excitation while a trapped particle can be scanned at different velocities. The measured dependence of the visibility of the MDR signal on the translation velocity indicates that a high sensitivity and a high scanning velocity of a trapped particle can be achieved simultaneously. A spectrally resolved image demonstrates that this technique provides an alternative imaging mechanism for near-field imaging and the surface tomography. REFERENCES H-M. Tzeng, K.F. Wall, M.B. Long and R.K. Chang, Laser emission from individual droplets at wavelengths corresponding to morphology-dependent resonances Opt. Lett. 9, , (1984). Proc. of SPIE Vol. 5514
5 2. M. Goppert-Mayer. Uber elementarakte mit zwei quantensprungen Ann. Phys. 9, , (1931). 3. D. Morrish, X. Gan and M. Gu, Observation of orthogonally polarized transverse electric and transverse magnetic oscillation modes in a microcavity excited by localized two-photon absorption Appl. Phys. Lett. 81, , (2002). 4. A. Ashkin and J.M. Dziedzic, Optical trapping and manipulation of viruses and bacteria Science 235, , (1987). 5. K. Svoboda, C.F. Schmidt, B.J. Schnapp and S.M. Block, Direct observation of kinesin stepping by optical trapping interferometry Nature 365, , (1993). 6. S.B. Smith, Y. Cui and C. Bustamante, Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules Science 271, , (1996). 7. M. Gu and P. Ke, Image enhancement in near-field scanning optical microscopy with laser-trapped metallicparticles Opt. Lett., 24, 74-76, (1999). 8. M. Gu and P. Ke, Effect of depolarization of scattered evanescent waves on particle-trapped near-field scanning optical microscopy Appl. Phys. Lett. 75, , (1999). 9. R.E. Benner, P.W. Barber, J.F. Owen and R.K. Chang, Observation of structure resonances in the fluorescence spectra from microspheres Phys. Rev. Lett. 44, , (1980). 10. A. Ashkin and J.M. Dziedzic, Observation of optical resonances of dielectric spheres by light scattering Appl. Opt. 20, , (1981). 11. A.J. Campillo, J.D. Eversole and H-B. Lin, Cavity quantum electrodynamic enhancement of stimulated emission in microdroplets Phys. Rev. Lett. 67, , (1991). 12. K. Sasaki, H. Fujiwara and H. Masuhara, Photon tunneling from an optically manipulated microsphere to a surface by lasing spectral analysis Appl. Phys. Lett. 70, , (1997). 13. R.K. Chang and A.J. Campillo, Optical Processes on Microcavities, (World Scientific, Singapore, 1996). 14. B. Jia, X. Gan and M. Gu, Height/width aspect ratio controllable two-dimensional sub-micron arrays fabricated with two-photon photopolymerization Optik, in press. (2004). Proc. of SPIE Vol
Morphology-dependent resonance induced by two-photon excitation in a micro-sphere trapped by a femtosecond pulsed laser
Morphology-dependent resonance induced by two-photon excitation in a micro-sphere trapped by a femtosecond pulsed laser Dru Morrish, Xiaosong Gan and Min Gu Centre for Micro-Photonics, School of Biophysical
More informationExact radiation trapping force calculation based on vectorial diffraction theory
Exact radiation trapping force calculation based on vectorial diffraction theory Djenan Ganic, Xiaosong Gan, and Min Gu Centre for Micro-Photonics, School of Biophysical Sciences and Electrical Engineering
More informationFemtosecond laser microfabrication in. Prof. Dr. Cleber R. Mendonca
Femtosecond laser microfabrication in polymers Prof. Dr. Cleber R. Mendonca laser microfabrication focus laser beam on material s surface laser microfabrication laser microfabrication laser microfabrication
More informationFundamentals of nanoscience
Fundamentals of nanoscience Spectroscopy of nano-objects Mika Pettersson 1. Non-spatially resolved spectroscopy Traditionally, in spectroscopy, one is interested in obtaining information on the energy
More informationSingle Emitter Detection with Fluorescence and Extinction Spectroscopy
Single Emitter Detection with Fluorescence and Extinction Spectroscopy Michael Krall Elements of Nanophotonics Associated Seminar Recent Progress in Nanooptics & Photonics May 07, 2009 Outline Single molecule
More informationSingle-beam optical fiber trap
Journal of Physics: Conference Series Single-beam optical fiber trap To cite this article: K Taguchi and N Watanabe 2007 J. Phys.: Conf. Ser. 61 1137 View the article online for updates and enhancements.
More informationPhotonic nanojet enhancement of backscattering of light by nanoparticles: a potential novel visible-light ultramicroscopy technique
Photonic nanojet enhancement of backscattering of light by nanoparticles: a potential novel visible-light ultramicroscopy technique Zhigang Chen and Allen Taflove Department of Electrical and Computer
More informationExperimental biophysics: Optical tweezer lab Supervisor: Stefan Holm,
Experimental biophysics: Optical tweezer lab :, stefan.holm@ftf.lth.se Written by Jason Beech & Henrik Persson, March 2009. Modified 2014 Karl Adolfsson, 2016 Experimental Biophysics: FAF010F, FYST23,
More informationFemtosecond laser applied to biophotonics. Prof. Cleber R. Mendonca
Femtosecond laser applied to biophotonics Prof. Cleber R. Mendonca introduction short pulse duration ö high intensity (even at low energy) introduction how short is a femtosecond pulse? 1fs= 10-15 s introduction
More informationDual-Wavelength Lasing from Organic Dye Encapsulated Metal-Organic Framework Microcrystals
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2019 Electronic Supplementary Information Dual-Wavelength Lasing from Organic Dye Encapsulated Metal-Organic
More informationOptics and Spectroscopy
Introduction to Optics and Spectroscopy beyond the diffraction limit Chi Chen 陳祺 Research Center for Applied Science, Academia Sinica 2015Apr09 1 Light and Optics 2 Light as Wave Application 3 Electromagnetic
More informationPolarization control of defect modes in threedimensional woodpile photonic crystals
Polarization control of defect modes in threedimensional woodpile photonic crystals Michael James Ventura and Min Gu* Centre for Micro-Photonics and Centre for Ultrahigh-bandwidth Devices for Optical Systems,
More informationWinter College on Optics and Energy February Optical nonlinearities in organic materials
2132-41 Winter College on Optics and Energy 8-19 February 2010 Optical nonlinearities in organic materials C.R. Mendonca University of Sao Paulo Brazil Optical nonlinearities in organic materials Prof.
More informationControlled Assembly of Organic Whispering Gallery Mode Microlasers as Highly Sensitive Chemical Sensors
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2017 Electronic Supplementary Information Controlled Assembly of Organic Whispering Gallery Mode Microlasers
More informationBackscattering enhancement of light by nanoparticles positioned in localized optical intensity peaks
Backscattering enhancement of light by nanoparticles positioned in localized optical intensity peaks Zhigang Chen, Xu Li, Allen Taflove, and Vadim Backman We report what we believe to be a novel backscattering
More informationMicrofabricação em materiais poliméricos usando laser de femtossegundos
Microfabricação em materiais poliméricos usando laser de femtossegundos Prof. Cleber R. Mendonça http://www.fotonica.ifsc.usp.br University of Sao Paulo - Brazil students 77.000 52.000 undergrad. 25.000
More informationOptical Tweezers. The Useful Micro-Manipulation Tool in Research
Optical Tweezers The Useful Micro-Manipulation Tool in Research Student: Nikki Barron Class: Modern Physics/ Biophysics laboratory Advisor: Grant Allen Instructor: David Kleinfeld Date: June 15, 2012 Introduction
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/NPHOTON.2013.97 Supplementary Information Far-field Imaging of Non-fluorescent Species with Sub-diffraction Resolution Pu Wang et al. 1. Theory of saturated transient absorption microscopy
More informationOPTICAL BISTABILITY AND UPCONVERSION PROCESSES IN ERBIUM DOPED MICROSPHERES
OPTICAL BISTABILITY AND UPCONVERSION PROCESSES IN ERBIUM DOPED MICROSPHERES J. Ward, D. O Shea, B. Shortt, S. Nic Chormaic Dept of Applied Physics and Instrumentation, Cork Institute of Technology,Cork,
More informationAdvanced techniques Local probes, SNOM
Advanced techniques Local probes, SNOM Principle Probe the near field electromagnetic field with a local probe near field probe propagating field evanescent Advanced techniques Local probes, SNOM Principle
More informationEnergy transport in metal nanoparticle plasmon waveguides
Energy transport in metal nanoparticle plasmon waveguides Stefan A. Maier, Pieter G. Kik, and Harry A. Atwater California Institute of Technology Thomas J. Watson Laboratory of Applied Physics, Pasadena,
More informationIntroduction to Nonlinear Optics
Introduction to Nonlinear Optics Prof. Cleber R. Mendonca http://www.fotonica.ifsc.usp.br Outline Linear optics Introduction to nonlinear optics Second order nonlinearities Third order nonlinearities Two-photon
More informationEnhancement mechanisms for optical forces in integrated optics
Enhancement mechanisms for optical forces in integrated optics M. L. Povinelli (a),m.lončar (b),e.j.smythe (b),m.ibanescu (c), S. G. Johnson (d), F. Capasso (b), and J. D. Joannopoulos (c) (a) Ginzton
More informationSUPPLEMENTARY INFORMATION
doi:10.1038/nature12036 We provide in the following additional experimental data and details on our demonstration of an electrically pumped exciton-polariton laser by supplementing optical and electrical
More informationMicrofibres for Quantum Optics. Dr Síle Nic Chormaic Quantum Optics Group
Microfibres for Quantum Optics Dr Síle Nic Chormaic Quantum Optics Group Motivation Strong need to engineer atoms and photons for the development of new technologies quantum technologies Future advances
More informationA microring multimode laser using hollow polymer optical fibre
PRAMANA c Indian Academy of Sciences Vol. 75, No. 5 journal of November 2010 physics pp. 923 927 A microring multimode laser using hollow polymer optical fibre M KAILASNATH, V P N NAMPOORI and P RADHAKRISHNAN
More informationSUPPLEMENTARY INFORMATION
Supplementary Information Speckle-free laser imaging using random laser illumination Brandon Redding 1*, Michael A. Choma 2,3*, Hui Cao 1,4* 1 Department of Applied Physics, Yale University, New Haven,
More informationNear Field Observation of a Refractive Index Grating and a Topographical Grating by an Optically Trapped Gold Particle
O-4R47 OPTICAL REVIEW Vol. 11, No. 6 (24) 1 Near Field Observation of a Refractive Index Grating and a Topographical Grating by an Optically Trapped Gold Particle Hiroo UKITA, Hirotaka UEMI y and Atsuhiro
More informationChapter 2 Physical Principle of Optical Tweezers
Chapter 2 Physical Principle of Optical Tweezers The radiation pressure of light was first deduced theoretically by James C. Maxwell in 1873 based on his electromagnetic theory [1, 2], and measured experimentally
More informationχ (3) Microscopic Techniques
χ (3) Microscopic Techniques Quan Wang Optical Science and Engineering University of New Mexico Albuquerque, NM 87131 Microscopic techniques that utilize the third order non-linearality (χ (3) ) of the
More informationSTM: Scanning Tunneling Microscope
STM: Scanning Tunneling Microscope Basic idea STM working principle Schematic representation of the sample-tip tunnel barrier Assume tip and sample described by two infinite plate electrodes Φ t +Φ s =
More informationLASER TRAPPING MICRO-PROBE FOR NANO-CMM
LASER TRAPPING MICRO-PROBE FOR NANO-CMM T. Miyoshi, Y. Takaya and S. Takahashi Division of Production and Measurement System Engineering Department of Mechanical Engineering and Systems Osaka University,
More informationAdministrative details:
Administrative details: Anything from your side? www.photonics.ethz.ch 1 Where do we stand? Optical imaging: Focusing by a lens Angular spectrum Paraxial approximation Gaussian beams Method of stationary
More informationScanning Tunneling Microscopy
Scanning Tunneling Microscopy References: 1. G. Binnig, H. Rohrer, C. Gerber, and Weibel, Phys. Rev. Lett. 49, 57 (1982); and ibid 50, 120 (1983). 2. J. Chen, Introduction to Scanning Tunneling Microscopy,
More informationLaser Tweezers and Other Advanced Physical Methods
Phys 6715 - Biomedical Physics Laser Tweezers and Other Advanced Physical Methods Yong-qing Li, PhD Department of Physics, East Carolina University Greenville, NC 27858, USA Email: liy@ecu.edu 1 Optical
More information2.1 Morphology dependent resonance
Literature Review 2.1 Morphology dependent resonance Optical cavities of two or more mirrors are utilised in all branches of modern linear and nonlinear optics. Practical usage of such cavities is technically
More informationTwo-Photon Fabrication of Three-Dimensional Metallic Nanostructures for Plasmonic Metamaterials
Two-Photon Fabrication of Three-Dimensional Metallic Nanostructures for Plasmonic Metamaterials Atsushi ISHIKAWA 1 and Takuo TANAKA 1,2 1- Metamaterials Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198,
More informationLaser Excitation Dynamics of Argon Metastables Generated in Atmospheric Pressure Flows by Microwave Frequency Microplasma Arrays
Physical Sciences Inc. Laser Excitation Dynamics of Argon Metastables Generated in Atmospheric Pressure Flows by Microwave Frequency Microplasma Arrays W.T. Rawlins, K.L. Galbally-Kinney, S.J. Davis Physical
More informationEnhancement of Exciton Transport in Porphyrin. Aggregate Nanostructures by Controlling. Hierarchical Self-Assembly
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2018 Supporting Information for Enhancement of Exciton Transport in Porphyrin Aggregate Nanostructures
More informationClass 1. Introduction to Nonlinear Optics
Class 1 Introduction to Nonlinear Optics Prof. Cleber R. Mendonca http://www.fotonica.ifsc.usp.br for a copy of this presentation www.fotonica.ifsc.usp.br ifsc presentations Outline Linear optics Introduction
More informationPROCEEDINGS OF SPIE. Nanoparticle sorting in silicon waveguide arrays. H. T. Zhao, Y. Zhang, L. K. Chin, P. H. Yap, K. Wang, et al.
PROCEEDINGS OF SPIE SPIEDigitalLibrary.org/conference-proceedings-of-spie Nanoparticle sorting in silicon waveguide arrays H. T. Zhao, Y. Zhang, L. K. Chin, P. H. Yap, K. Wang, et al. H. T. Zhao, Y. Zhang,
More informationLaboratory 3: Confocal Microscopy Imaging of Single Emitter Fluorescence and Hanbury Brown, and Twiss Setup for Photon Antibunching
Laboratory 3: Confocal Microscopy Imaging of Single Emitter Fluorescence and Hanbury Brown, and Twiss Setup for Photon Antibunching Jonathan Papa 1, * 1 Institute of Optics University of Rochester, Rochester,
More informationOptical tweezers. SEMINAR 1b - 1. LETNIK, II. STOPNJA. Author: Matevž Majcen Hrovat. Mentor: prof. dr. Igor Poberaj. Ljubljana, May 2013.
SEMINAR 1b - 1. LETNIK, II. STOPNJA Optical tweezers Author: Matevž Majcen Hrovat Mentor: prof. dr. Igor Poberaj Ljubljana, May 2013 Abstract The seminar introduces the physics of optical trapping and
More informationRichard Miles and Arthur Dogariu. Mechanical and Aerospace Engineering Princeton University, Princeton, NJ 08540, USA
Richard Miles and Arthur Dogariu Mechanical and Aerospace Engineering Princeton University, Princeton, NJ 08540, USA Workshop on Oxygen Plasma Kinetics Sept 20, 2016 Financial support: ONR and MetroLaser
More informationNear-field imaging and spectroscopy of electronic states in single-walled carbon nanotubes
Early View publication on www.interscience.wiley.com (issue and page numbers not yet assigned; citable using Digital Object Identifier DOI) Original phys. stat. sol. (b), 1 5 (2006) / DOI 10.1002/pssb.200669179
More informationConfocal Microscopy Imaging of Single Emitter Fluorescence and Hanbury Brown and Twiss Photon Antibunching Setup
1 Confocal Microscopy Imaging of Single Emitter Fluorescence and Hanbury Brown and Twiss Photon Antibunching Setup Abstract Jacob Begis The purpose of this lab was to prove that a source of light can be
More informationLaser trapping dynamics of 200 nm-polystyrene particles at a solution surface
Laser trapping dynamics of 200 nm-polystyrene particles at a solution surface Ken-ichi Yuyama a, Teruki Sugiyama* b, Hiroshi Masuhara* a a Department of Applied Chemistry and Institute of Molecular Science,
More informationScanning Near-Field Infrared Microscopy (SNFIM) LPC, Newport News, VA, January 17, Edward Gillman
Scanning Near-Field Infrared Microscopy (SNFIM) LPC, Newport News, VA, January 17, 00 Edward Gillman (gillman@jlab.org) Scanning Near-Field Optical Microscope (SNOM) The scanning near-field optical microscope
More informationOptical Tweezers. BGGN 266, Biophysics Lab. June, Trygve Bakken & Adam Koerner
Optical Tweezers BGGN 266, Biophysics Lab June, 2009 Trygve Bakken & Adam Koerner Background There are a wide variety of force spectroscopy techniques available to investigators of biological systems.
More informationCombining High Resolution Optical and Scanning Probe Microscopy
Combining High Resolution Optical and Scanning Probe Microscopy Fernando Vargas WITec, Ulm, Germany www.witec.de Company Background Foundation 1997 by O. Hollricher, J. Koenen, K. Weishaupt WITec = Wissenschaftliche
More informationApplications of field-enhanced near-field optical microscopy
Applications of field-enhanced near-field optical microscopy A. Bouhelier, M. R. Beversluis, and L. Novotny The Institute of Optics, University of Rochester, Rochester, NY 14627, U.S.A Abstract Metal nanostructures
More informationCoherent control of light matter interaction
UNIVERSIDADE DE SÃO PAULO Instituto de Física de São Carlos Coherent control of light matter interaction Prof. Dr. Cleber Renato Mendonça Photonic Group University of São Paulo (USP), Institute of Physics
More informationOptical Trapping Force on a Plasmonic Substrate
Yu Pan Master of Science Thesis Supervisor: Prof. Min Yan(KTH) Examiner: Prof. Min Qiu(KTH) TRITA-ICT-EX-2012: 107 Abstract Optical trapping is currently widely applied in the field of biotechnology, in
More informationSpectroscopies for Unoccupied States = Electrons
Spectroscopies for Unoccupied States = Electrons Photoemission 1 Hole Inverse Photoemission 1 Electron Tunneling Spectroscopy 1 Electron/Hole Emission 1 Hole Absorption Will be discussed with core levels
More informationPROCEEDINGS OF SPIE. Sorting and measurement of single gold nanoparticles in an optofluidic chip
PROCEEDINGS OF SPIE SPIEDigitalLibrary.org/conference-proceedings-of-spie Sorting and measurement of single gold nanoparticles in an optofluidic chip Y. Z. Shi, S. Xiong, Y. Zhang, L. K. Chin, J. H. Wu,
More informationVisualization of Xe and Sn Atoms Generated from Laser-Produced Plasma for EUV Light Source
3rd International EUVL Symposium NOVEMBER 1-4, 2004 Miyazaki, Japan Visualization of Xe and Sn Atoms Generated from Laser-Produced Plasma for EUV Light Source H. Tanaka, A. Matsumoto, K. Akinaga, A. Takahashi
More informationTorque transfer in optical tweezers due to orbital angular momentum
Torque transfer in optical tweezers due to orbital angular momentum Simon J. W. Parkin, Gregor Knöner, Timo A. Nieminen, Norman R. Heckenberg and Halina Rubinsztein-Dunlop Centre for Biophotonics and Laser
More informationPolarization control and sensing with two-dimensional coupled photonic crystal microcavity arrays. Hatice Altug * and Jelena Vučković
Polarization control and sensing with two-dimensional coupled photonic crystal microcavity arrays Hatice Altug * and Jelena Vučković Edward L. Ginzton Laboratory, Stanford University, Stanford, CA 94305-4088
More informationDept. of Physics, MIT Manipal 1
Chapter 1: Optics 1. In the phenomenon of interference, there is A Annihilation of light energy B Addition of energy C Redistribution energy D Creation of energy 2. Interference fringes are obtained using
More informationSingle Semiconductor Nanostructures for Quantum Photonics Applications: A solid-state cavity-qed system with semiconductor quantum dots
The 3 rd GCOE Symposium 2/17-19, 19, 2011 Tohoku University, Sendai, Japan Single Semiconductor Nanostructures for Quantum Photonics Applications: A solid-state cavity-qed system with semiconductor quantum
More informationCreating and probing of a perfect vortex in situ with an optically trapped particle
Creating and probing of a perfect vortex in situ with an optically trapped particle Mingzhou Chen, Michael Mazilu, Yoshihiko Arita, Ewan M. Wright, and Kishan Dholakia, SUPA, School of Physics & Astronomy,
More informationSupplementary Information
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2014 Supplementary Information Large-scale lithography-free metasurface with spectrally tunable super
More informationThis document contains the following supporting information: 1. Wide field scanning electron microscope image
Supporting information for Self-assembled nanoparticle dimer antennas for plasmonic-enhanced single-molecule fluorescence detection at micromolar concentrations Deep Punj, Raju Regmi, Alexis Devilez, Robin
More informationSet-up for ultrafast time-resolved x-ray diffraction using a femtosecond laser-plasma kev x-ray-source
Set-up for ultrafast time-resolved x-ray diffraction using a femtosecond laser-plasma kev x-ray-source C. Blome, K. Sokolowski-Tinten *, C. Dietrich, A. Tarasevitch, D. von der Linde Inst. for Laser- and
More informationAluminum for nonlinear plasmonics: Methods Section
Aluminum for nonlinear plasmonics: Methods Section Marta Castro-Lopez, Daan Brinks, Riccardo Sapienza, and Niek F. van Hulst, ICFO - Institut de Ciencies Fotoniques, and ICREA - Institució Catalana de
More informationSpectroscopic investigations of Rb- and Cs- rare gas systems
Spectroscopic investigations of Rb- and Cs- rare gas systems S. J. Davis *, W. T. Rawlins, K. L. Galbally-Kinney, and W.J. Kessler Physical Sciences Inc., 20 New England Business Center, Andover, MA 01810
More informationCHAPTER 7 SUMMARY OF THE PRESENT WORK AND SUGGESTIONS FOR FUTURE WORK
161 CHAPTER 7 SUMMARY OF THE PRESENT WORK AND SUGGESTIONS FOR FUTURE WORK 7.1 SUMMARY OF THE PRESENT WORK Nonlinear optical materials are required in a wide range of important applications, such as optical
More informationContinuous-wave biexciton lasing at room temperature using solution-processed quantum wells
CORRECTION NOTICE Continuous-wave bieciton lasing at room temperature using solution-processed quantum wells Joel Q. Grim, Sotirios Christodoulou, Francesco Di Stasio, Roman Krahne, Roberto Cingolani,
More informationFast and Slow Ligand Exchange at the Surface of Colloidal Gold Nanoparticles
Fast and Slow Ligand Exchange at the Surface of Colloidal Gold Nanoparticles Rebecca Dinkel 1, Björn Braunschweig 1,2 * and Wolfgang Peukert 1,2 1 Institute of Particle Technology (LFG), Friedrich-Alexander
More informationThis document is downloaded from DR-NTU, Nanyang Technological University Library, Singapore.
This document is downloaded from DR-NTU, Nanyang Technological University Library, Singapore. Title Optical alignment of a cylindrical object Author(s) Citation Song, Chaolong; Nguyen, Nam-Trung; Asundi,
More informationLaser. emission W FL
W Laser Laser emission W FL Supplementary Figure 1. Comparison between fluorescence and laser emission spectra. The fluorescence has broad spectrum whereas the laser has very narrow spectrum. W FL and
More informationPROCEEDINGS OF SPIE. Scaling rules for the design of a narrow-band grating filter at the focus of a free-space beam
PROCEEDINGS OF SPIE SPIEDigitalLibrary.org/conference-proceedings-of-spie Scaling rules for the design of a narrow-band grating filter at the focus of a free-space beam Eanuel Bonnet, Alain Cachard, Alexandre
More informationHYPER-RAYLEIGH SCATTERING AND SURFACE-ENHANCED RAMAN SCATTERING STUDIES OF PLATINUM NANOPARTICLE SUSPENSIONS
www.arpapress.com/volumes/vol19issue1/ijrras_19_1_06.pdf HYPER-RAYLEIGH SCATTERING AND SURFACE-ENHANCED RAMAN SCATTERING STUDIES OF PLATINUM NANOPARTICLE SUSPENSIONS M. Eslamifar Physics Department, BehbahanKhatamAl-Anbia
More informationNear-Field Nano/Atom Optics and Technology
M. Ohtsu (Ed.) Near-Field Nano/Atom Optics and Technology With 189 Figures / Springer Preface List of Contributors V VII XIII 1. Introduction 1 1.1 Near-Field Optics and Related Technologies 1 1.2 History
More informationNano fabrication and optical characterization of nanostructures
Introduction to nanooptics, Summer Term 2012, Abbe School of Photonics, FSU Jena, Prof. Thomas Pertsch Nano fabrication and optical characterization of nanostructures Lecture 12 1 Optical characterization
More informationLecture 11, May 11, 2017
Lecture 11, May 11, 2017 This week: Atomic Ions for QIP Ion Traps Vibrational modes Preparation of initial states Read-Out Single-Ion Gates Two-Ion Gates Introductory Review Articles: D. Leibfried, R.
More informationSupplementary Information for. Vibrational Spectroscopy at Electrolyte Electrode Interfaces with Graphene Gratings
Supplementary Information for Vibrational Spectroscopy at Electrolyte Electrode Interfaces with Graphene Gratings Supplementary Figure 1. Simulated from pristine graphene gratings at different Fermi energy
More informationSolution set for EXAM IN TFY4265/FY8906 Biophysical microtechniques
ENGLISH NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY DEPARTMENT OF PHYSICS Contact during exam: Magnus Borstad Lilledahl Telefon: 73591873 (office) 92851014 (mobile) Solution set for EXAM IN TFY4265/FY8906
More informationNanoscale confinement of photon and electron
Nanoscale confinement of photon and electron Photons can be confined via: Planar waveguides or microcavities (2 d) Optical fibers (1 d) Micro/nano spheres (0 d) Electrons can be confined via: Quantum well
More information5. 3P PIV Measurements
Micro PIV Last Class: 1. Data Validation 2. Vector Field Operator (Differentials & Integrals) 3. Standard Differential Scheme 4. Implementation of Differential & Integral quantities with PIV data 5. 3P
More informationExcitation Efficiency of a Morphology-Dependent Resonance by a Focused Gaussian Beam
Cleveland State University EngagedScholarship@CSU Physics Faculty Publications Physics Department 1-1-1998 Excitation Efficiency of a Morphology-Dependent Resonance by a Focused Gaussian Beam James A.
More informationSUB-NATURAL-WIDTH N-RESONANCES OBSERVED IN LARGE FREQUENCY INTERVAL
SUB-NATURAL-WIDTH N-RESONANCES OBSERVED IN LARGE FREQUENCY INTERVAL A. KRASTEVA 1, S. GATEVA 1, A. SARGSYAN 2, D. SARKISYAN 2 AND S. CARTALEVA 1 1 Institute of Electronics, Bulgarian Academy of Sciences,
More informationSTM spectroscopy (STS)
STM spectroscopy (STS) di dv 4 e ( E ev, r) ( E ) M S F T F Basic concepts of STS. With the feedback circuit open the variation of the tunneling current due to the application of a small oscillating voltage
More informationCollective effects in second-harmonic generation from plasmonic oligomers
Supporting Information Collective effects in second-harmonic generation from plasmonic oligomers Godofredo Bautista,, *, Christoph Dreser,,, Xiaorun Zang, Dieter P. Kern,, Martti Kauranen, and Monika Fleischer,,*
More informationOptics of complex micro structures
Optics of complex micro structures dielectric materials λ L disordered partially ordered ordered random multiple scattering liquid crystals quasi crystals (Fibonacci) photonic crystals Assembly of photonic
More informationUniversità degli Studi di Bari "Aldo Moro"
Università degli Studi di Bari "Aldo Moro" Table of contents 1. Introduction to Atomic Force Microscopy; 2. Introduction to Raman Spectroscopy; 3. The need for a hybrid technique Raman AFM microscopy;
More informationPhys Biomedical Physics: Yong-qing Li, PhD. Department of Physics, East Carolina University Greenville, NC 27858, USA.
Phys 6715 - Biomedical Physics: Optical Trapping in Air Yong-qing Li, PhD Department of Physics, East Carolina University Greenville, NC 27858, USA Email: liy@ecu.edu 1 Outline 1. Why optical trapping
More informationScanning Probe Microscopy. Amanda MacMillan, Emmy Gebremichael, & John Shamblin Chem 243: Instrumental Analysis Dr. Robert Corn March 10, 2010
Scanning Probe Microscopy Amanda MacMillan, Emmy Gebremichael, & John Shamblin Chem 243: Instrumental Analysis Dr. Robert Corn March 10, 2010 Scanning Probe Microscopy High-Resolution Surface Analysis
More information1. Fabrication. Lukáš Ondič a, Marian Varga a, Karel Hruška a, Jan Fait a,b and Peter Kapusta c
Supporting information to Enhanced Extraction of Silicon-Vacancy Centers Light Emission Using Bottom-Up Engineered Polycrystalline Diamond Photonic Crystal Slabs Lukáš Ondič a, Marian Varga a, Karel Hruška
More informationLecture 6. Alternative storage technologies. All optical recording. Racetrack memory. Topological kink solitons. Flash memory. Holographic memory
Lecture 6 Alternative storage technologies All optical recording Racetrack memory Topological kink solitons Flash memory Holographic memory Millipede Ferroelectric memory All-optical recording It is possible
More informationLight Interaction with Small Structures
Light Interaction with Small Structures Molecules Light scattering due to harmonically driven dipole oscillator Nanoparticles Insulators Rayleigh Scattering (blue sky) Semiconductors...Resonance absorption
More informationLow-Frequency Raman Spectra of Carbon Nanotubes Measured with an Astigmatism-Free Schmidt-Czerny-Turner Spectrograph
Low-Frequency Raman Spectra of Carbon Nanotubes Measured with an Astigmatism-Free Schmidt-Czerny-Turner Spectrograph Abstract Traditional Czerny-Turner (CT) spectrographs suffer from the optical aberration
More informationStrong focusing higher-order laser modes: transverse and longitudinal optical fields
Journal of Physics: Conference Series PAPER OPEN ACCESS Strong focusing higher-order laser modes: transverse and longitudinal optical fields To cite this article: A V Kharitonov and S S Kharintsev 015
More informationPEEM and XPEEM: methodology and applications for dynamic processes
PEEM and XPEEM: methodology and applications for dynamic processes PEEM methods and General considerations Chemical imaging Magnetic imaging XMCD/XMLD Examples Dynamic studies PEEM and XPEEM methods 1
More informationObservation of spectral enhancement in a soliton fiber laser with fiber Bragg grating
Observation of spectral enhancement in a soliton fiber laser with fiber Bragg grating L. M. Zhao 1*, C. Lu 1, H. Y. Tam 2, D. Y. Tang 3, L. Xia 3, and P. Shum 3 1 Department of Electronic and Information
More informationFinal Report for AOARD grant FA Measurement of the third-order nonlinear susceptibility of graphene and its derivatives
Final Report for AOARD grant FA2386-12-1-4095 Measurement of the third-order nonlinear susceptibility of graphene and its derivatives Principal investigator: A/Prof. Tang Dingyuan Division of Microelectronics
More information3D SUPER-RESOLUTION FLUORESCENCE MICROSC- OPY USING CYLINDRICAL VECTOR BEAMS
Progress In Electromagnetics Research Letters, Vol. 43, 73 81, 2013 3D SUPER-RESOLUTION FLUORESCENCE MICROSC- OPY USING CYLINDRICAL VECTOR BEAMS Taikei Suyama 1 and Yaoju Zhang 2, * 1 Department of Electrical
More informationResonance Raman measurements utilizing a deep UV source
Resonance Raman measurements utilizing a deep UV source Adam Willitsford a, C. Todd Chadwick b, Hans Hallen b, and C. Russell Philbrick a a The Pennsylvania State University, Department of Electrical Engineering,
More informationExperimental Optics. Optical Tweezers. Contact: Dr. Robert Kammel, Last edition: Dr. Robert Kammel, February 2016
Experimental Optics Contact: Dr. Robert Kammel, e-mail: Robert.Kammel@uni-jena.de Last edition: Dr. Robert Kammel, February 2016 Optical Tweezers Contents 1 Overview 2 2 Safety Issues 2 3 Theoretical background
More informationHow to measure packaging-induced strain in high-brightness diode lasers?
How to measure packaging-induced strain in high-brightness diode lasers? Jens W. Tomm Max-Born-Institut für Nichtlineare Optik und Kurzzeitspektroskopie Berlin Max-Born-Str. 2 A, D-12489 Berlin, Germany
More information