Self Cleaning Ion Source and other Innovations in Mass Spectrometry. Kirk Lokits (GCMS Applications Chemist) Blacksburg, VA February 2 nd, 2017

Transcription

1 Self Cleaning Ion Source and other Innovations in Mass Spectrometry Kirk Lokits (GCMS Applications Chemist) Blacksburg, VA February 2 nd,

2 Jet Clean! Outcome JetClean self-cleaning ion source technology Reduces cleaning up to 80%. Run more samples! 2

3 Main features Carefully controlled H 2 flow introduction to the MS at very low flow rates Simple, integrated software Set-up as simple as a pressure pulsed injection. Now only in MassHunter. Mass Flow Controller (MFC) Parameters saved in the method, included log file, readily transportable

4 Rhodamine 6G Treated Lens: Before & After Cleaning MW: 477 Artificial contamination After JetClean After He Bakeout The heavy contamination was removed where lens was exposed to H 2. The remaining dark inked areas are consistent with the masking by the ion volume step Same treatment using only He (bake out) did not clean the surface

5 Modes of Operation: General Applications Clean only (Batch or offline mode) Analysis Helium Validated Applications Acquire and Clean (Concurrent or online mode) Cleaning Helium Hydrogen Helium Analysis Hydrogen EI/CI mode EI only mode Potential interaction No Interaction

6 Successful Applications (so far) Acquire and Clean On-line Operation Available configuration *PAHs TQ and SQ 400μl H2, river water/sediment, (also in edible oil) Cleaning frequency monthly prior, DID NOT CLEAN since SCIS installation (2years+) *Pyrolysis (SQ) Polymers 200μl H2 Cleaning frequency monthly prior, DID NOT CLEAN since SCIS installation (1+ year) Phthalates (SQ) in alcoholic beverages 300ul H2 (LFS/China ATS) Arsine/Phosphine fg level (SQ HES) pure ethylene and propylene (120m, 8u film is needed for separation!) Pesticides Environmental, 7000C, PCB, PBDE, OCP, 200μl H2, Water/sediment DID NOT CLEAN since SCIS installation (3.5 months) in Clean Only Off-line or Batch Mode Operation Promoted configuration Forensic Toxicology, SQ Cleaning frequency reduced to 1/10 (~ 1 / year) Common toxicology screen QC maintained *Food analysis, TQ Cleaning frequency reduced to 1/6 (~2 / year) 3h stabilization needed after cleaning 160 pesticides in botanicals, ppb SANCO QC maintained *Pesticide Residue in honey 7010 (LFS) MDLs reduced by 50%, data collection ongoing

7 The Most Powerful EI GC/MS Source Ever Made. More intense electron beam NEW! 5977B High Efficiency Source X Longer path length for electron beam/effluent interaction = Up to 30x More Ions Produced 5977A Extractor Source 5977B High Efficiency Source with Magnet Removed 7

8 The Most Powerful EI GC/MS Source Ever Made. How the HES works High emission filaments for more electron current Powerful cylindrical magnet collimates electrons Long path length for ionization 8

9 The Most Powerful EI GC/MS Source Ever Made. These values must be met at instrument install Conventional EI source on 7000C and 5977A 4 fg OFN IDL 7000C <10 fg OFN IDL 5977A High Efficiency Source on Agilent 7010 and 5977B 0.5 fg OFN IDL fg OFN IDL 5977B 9

10 In a ultra low noise environment A small noise variation creates a big SNR variation 8 consecutive injections of 1 μl, 100fg/μl OFN. The peaks virtually overlap; the signal area and height values are very consistent. The baseline noise is very low for every plot, but the automatically selected RMS noise values vary in a wide range, yielding the values below for the 8 OFN injections: 8 OFN runs Area Noise Minimum (for each value) Maximum (for each value) Average (for each value) SNR (Height/noise) % RSD The area values of the response of the injections would not indicate a factor of 100 fold difference in SNR. But a small difference in the ultra low noise baseline can result in extreme difference in the calculated SNR. 10

11 Detection Limit as Defined by Basic Statistics DL = t * SD DL where is the detection limit t is the student t value, given for the desired confidence level (99%) and degree of freedom (# of repeated measurements -1) SD SD is the standard deviation of the value measured Amount measured should be only 5-10X higher than the DL This can be applied in any discipline, not just analytical chemistry 11

12 Calculating IDL (Instrument Detection Level) on a 5977A t α, n-1 value for 99% confidence level and 8 injection is SD is determined from the standard deviation of the area count of 8 consecutive injections of 1 µl of 100fg/µl OFN std. - This would give us IDL based on area. Much more useful if we express this value based on concentration. IDL (area) = 2.988* SD (area) %RSD Average area count represents 100fg injected amount IDL 100fg IDL 12

13 Reimagine What Can Be Done with Single Quad GC/MS Extremely Low Limits of Detection Rock Solid Precision Exceptional Science -- Analyze and Identify Trace Compounds Previously Unseen with Single Quad GC/MS 10 fg OFN injections LOD = 0.57 fg calculated from 8 consecutive injections Overlay of 8 injections of 2pg of pyrene: Do more with Less Sample Achieve today s analytical sensitivity using a fraction of the sample Minimized Environmental Impact 13

14 Benefits That Touch Your Entire Operation Decreased Shipping Costs Less Frequent Liner Maintenance Decreased Storage Costs Longer Column Life 1 Liter 100 ml Decreased Disposal Costs Faster Sample Prep Push past the analytical frontier do amazing science in new ways Ship, store and dispose of a fraction of your current, valuable sample volume Less organic solvent to buy, store, and dispose of A cleaner system means less downtime for cleaning and maintenance Less Sample Injected = Cleaner System 14

15 The Agilent 7010B Triple Quadrupole GC/MS System More Ions Than Ever Before Features a completely new EI source design produces 20-30X more ions Instrument Detection Limit: 0.5 fg OFN 8X better than 7000D Joins the 7000D, the best selling GC/MS/MS system ever 15

16 The 7010B doesn t just look different 16

17 Benefits of new design shorter dwell times! Ultra-short Dwell Times More transitions per compound Weaker transitions can be used for better selectivity 17

18 Benefits of new design more transitions to choose from! Use of Alternate Transitions Better Selectivity Against Interferences Quant transition shows interference 2 nd most abundant transition measured on: C + MRM ( > 107.0) 0pt5ppb_PU_ _04.D Smooth x min Acquisition Time (min) + MRM ( > 137.0) 0pt5ppb_PU_ _04.D Smooth x min Acquisition Time (min) 0.5 ppb in Plum 1.1% Area MRM ( > 137.0) FLAG_Inst1_PU_0pt5ppb_70 x min Acquisition Time (min) 0.6% Area

19 Benefits That Touch Your Entire Operation Decreased Shipping Costs Less Frequent Liner Maintenance Decreased Storage Costs Longer Column Life 1 Liter 100 ml Decreased Disposal Costs Faster Sample Prep Push past the analytical frontier do amazing science in new ways Ship, store and dispose of a fraction of your current, valuable sample volume Less organic solvent to buy, store, and dispose of A cleaner system means less downtime for cleaning and maintenance Less Sample Injected = Cleaner System 19

20 The 7010B doesn t just look different Cost Breakdown and Cost Savings for Sample Preparation with QuEChERS and Mini-QuEChERS Technique Sample Preparation Cost/Sample Centrifuge Tube ACN Salts Internal Standards: Captan-d6, Folpet-d4 dspe General F&V or Universal Total Cost/ Sample Cost Savings QuEChERS $0.43 $1.50 $2.96 $0.30 $1.32/$1.96 $6.51/$ Mini-QuEChERS $0.42 $0.20 $0.80 $0.04 $1.32/$1.96 $2.78/$ % 48% LOQs for the majority of challenging pesticides in foods are 1 ng/g despite injecting less sample from a smaller aliquot in order to improve method robustness. Cost savings are realized through both scaled down sample prep and decreased instrument maintenance. This was made possible by using a miniaturized QuEChERS method and a High Efficiency Source for GC/MS/MS analysis. 20

21 HES Conclusions High Efficiency Source (HES) 5977B Improves performance with high sensitivity Extractor Ion Source 1.5 fg OFN EI specification More ecological reduce sample aliquot size to reduce consumption of solvents, sample prep materials, disposal costs, etc. 7000D RoHS (Restriction of Hazardous Substances) compliance Dynamic MRM SIM/Scan mode 7010B Improves performance with high efficiency source 20 30% x increase in ion flux 0.5 fg OFN EI specification Allows use of less abundance transitions 21

22 New Features added to the 7000 Series Instruments Dynamic MRM (dmrm) Retention time Compound 1 Compound 2 Or Compound 1 Compound Retention window Compound 3 2 Compound 3 5 min. Time Segment (TS) 6 min. 5 min. dmrm 6 min. Create and edit methods more easily no need to constantly change time segment boundaries Obtain greater measurement efficiency measure fewer compounds simultaneously; dwell times automatically increase whenever possible Continue to use existing TS-based methods, or convert them to dmrm at your own pace 22

23 New Features added to the 7000 Series Instruments Dynamic MRM (dmrm) Easily find and fix highly congested spots by decreasing retention windows Agilent RT locking makes it possible Select desired data rate here dwell times automatically adjust here 23

24 New Features added to the 7000 Series Instruments SIM/Scan: It s Like Having Another Single Quad Whenever You Need One! It s this easy SQ SIM table automatically recreated, so you measure the same compounds at the same time, with the same dwell times as on your SQ Works for scan methods, too Simultaneous SIM/scan capability, of course! 24

25 What is High Mass Accuracy and why is it useful? High mass accuracy means that you can measure a mass out to the 4 th decimal place (e.g., ) is useful for: Deriving a molecular formula and dramatically reducing the number of candidates (unknown ID) Providing higher confidence for targets Elucidating fragmentation patterns (esp. with MS/MS)

26 What is High Resolution..and why is it useful? High resolution means that it can resolve mass peaks that are of the same nominal mass is useful for: Distinguishing co-eluting compounds (with equal nominal mass) Selectivity in complex matrix Improved mass accuracy (narrow mass peaks)

27 What is High Resolution and Accurate Mass? Low Resolution/Mass Accuracy High Resolution/Mass Accuracy m/z = 614 W 1/2 = Da 1 Da Resolution = (m/z)/w 1/2 Mass accuracy Theoretical Theoretical - Experimental 10 PFTBA (C 12 F 24 N) = R = 614/0.68 = 903 = (( )/ )x10 6 = ppm R = 614/ = 14,522 = (( )/ )x10 6 = ppm

28 High Res can Distinguish Co-eluting Peaks These 2 compounds appears as single m/z 240 peak for any unit mass resolution MS Flurenol methyl ester m/z = Mass error = 1.7 ppm Δ m/z = Dimetilan m/z = Mass error = 0.4 ppm ~13,500 resolution FWHM No Internal Reference Mass (IRM) corrections applied

29 Q-TOF can Eliminate Matrix Interference Okra QuEChERS Extract +/- 0.5 amu (~ 300ppm) b-tocopherol Indoxacarb Low Resolution Analyte Indoxacarb ion (100 pg) at m/z (fragment ion) Matrix interference ion (b-tocopherol) at m/z High Resolution Matrix (b-tocopherol) interference eliminated in MS mode +/- 5 ppm Indoxacarb MS/MS can be used for even more selectivity

30 Improved Mass Accuracy and Stability Average mass error: 0.95 ppm for 50 consecutive injections -1.2 ppm -0.3 ppm -1.5 ppm -1.3 ppm 2.3 ppm 0.9 ppm The 7200B GC/Q-TOF exhibits excellent mass accuracy Average mass error for ~ 50 injections of OFN over a course of 5 hours is 0.95 ppm

31 7200B Specifications

32 Agilent s Programmable Inlet MultiMode Inlet (MMI) 7890 standard pneumatics 7890 turn-top Uses 7890 S/SL liners, septa and o-rings 7890 standard capillary fitting 32

33 MMI Inlet Features and Operation Split\Splitless and Multimode Similarities Turn Top Insert Weldment Use the same liners/o-rings Use Standard Column Nuts Pneumatic Controls are identical Unique Multimode Features No Gold Seal Rapid Heating up to 900 degrees C/minute Liquid CO 2 or N 2 for Inlet cooling Solvent Vent Mode for Large Volume Injection Cool/Temperature Programmed Split or Splitless Modes Heater/Thermocouple welded to inlet body 150 Watt Heater Translator Board for Thermocouple

34 MMI Inlet Large Volume Injection Solvent Vent Calculators Solvent Vent Calculator Instrument Utilities Solvent Elimination Calculator Wizard

35 MMI Inlet Hardware Inlet Body Heater and Thermocouple: Integral part of the Inlet Body For very rapid Heating Thermocouple Connection 150 Watt Heater The wall of the inlet body is only thick for rapid heat transfer

36 MMI Inlet for LN 2 Cooling Cryo Feed Tube 1/4 Flex Tubing for LN2 Carrier Tube Split Vent Tube Septum Purge Tube

37 Multimode Inlet Features Hardware Temperature range of -160C to 450C 15C/sec (900C/min) Injection Modes: Hot S/SL, Cold S/SL, all in pulsed mode, solvent vent mode, residue removal mode 37

38 Backflush to remove non-volatile residues 38

39 POST RUN Back flush Capillary Column 1 Capillary Column 2

40 Concurrent Back flush Column m x 0.18 Pre-Column Column 2 LTM Analytical Column

41 Back flush Coated Pre-column.

42 PCBs in Fish Oil. GC Analysis with ECD - Residual Sample Matrix Affects RTs N or m Runs without Backflushing Retention times shift ~4-5 sec during 10 runs m in N o r m Runs with Backflushing m in

43 The Value of G1701FA Software All Inclusive G1701FA Software Package MassHunter GC/MS Acquisition MassHunter Quantitative Analysis MassHunter Qualitative Analysis MSD ChemStation Data Analysis 21CFR11 Compliance tools (Acquisition and MH Quantitative Analysis) GC/MS and LC/MS Translators MassHunter Qual MSD ChemStation DA Collated User Information Workflow guides, Video Tutorials, all in one location MassHunter Quant Support 1yr Upgrade, Updates, and Phone Support Training and Familiarization options 43

44 Migrate MSD ChemStation Data to MassHunter Data MassHunter Quant MSD ChemStation (.D) Data MassHunter Qual WorkStation (.SMS) Data Generic (.CDF) 44

45 Migrate MSD ChemStation Quantitative Methods to MassHunter MassHunter Quant Method MSD ChemStation (QDB.MTH) Method WorkStation (.mth) 45

46 MassHunter A Software Suite MassHunter software is modular: Different modules that focus on these specific tasks: Acquisition (Instrument Specific) Quantitative Analysis (Quant) Qualitative Analysis (Qual) A common reporting engine Suite of accessory applications: Unknown Analysis: batch versions of deconvolution and library searching Library editor Method development assistants Data Translators

47 MassHunter Data Analysis Software MassHunter s main data analysis modules have different functions: Quantitative (Quant): (Target Analysis - Quantitation) Review Batch results Visualize quality outliers Powerful quantitation engine Compounds-at-a-Glance Library Editor Unknowns Analysis Qualitative (Qual): (Discovery of non-target compounds or features, & Method Development) Finds compounds Identifies compounds Uses chromatogram and spectra extraction tools, i.e. deconvolution Searches El Libraries

48 MassHunter WorkStation Qualitative Analysis Data Navigator Chromatogram Results Method Explorer Method Editor Spectrum Results

49 Find Compound by Chromatogram Deconvolution Agilent deconvolution algorithm, similar to AMDIS, integrated into MassHunter Creates Compounds with chromatographic and spectral components

50 Quantitative Analysis Screen The Quant Batch screen looks like this. Results Information for the batch can be displayed. Visual guides highlight specific data that fall outside specific, predefined conditions. Compound Information displays graphical representation of the peak, qualifier information, spectral information, and the ISTD. Visual guides help identify associated data problems. Batch Information provides easy visualization and customization of relevant desired data. Compound Information Results Information Batch Information (i.e., Calibration Curve)

51 Overview of Unknowns Analysis Quant addresses target compound list What else is present in the samples? Quantitation Ion Extraction Deconvolution and Unknowns Analysis Library Search

52 Quant & Unknowns Analysis Workflow Chromatogram MS Scan data Chromatogram Integrate Integrate Quantitation 1 Extract 2 Deconvolute Spectra Target Match 3 Library Match 4 5

53 Unknowns Analysis Workflow Process batch of samples in Quantitative Analysis Run Unknowns Analysis Analyze Perform deconvolution to create components Perform library matching on components, assign compound ID Link components to Quant target compounds from Assign estimated concentrations to non target components Review data View component and library spectra View molecular structure vs. spectrum View ion peak shapes vs. component peak shape vs. TIC Edit components (change compound ID, delete) Run queries for custom review Print Report

54 Unknowns Analysis

55 Chromatogram Chromatogram displays selected component(s) in Blue All non-selected ions (EIC) displayed in Green TIC displayed in Black Clicking in the Chromatogram display selects the nearest peak and selects it in Components table

56 Spectrum & Ion Peaks Default number ions displayed in Ions Peaks is 5, limit is 10 Click on ion in Spectrum to make it add / remove in Ion Peaks Click on label in Spectrum or Ion Peaks to remove Quant Method Qualifier Target

57 Training Resource - Familiarization Guide Familiarization Guide in English Japanese Chinese Familiarization guide covers basic operations Examples use example data on media 57 February 3,

58 Questions Thank you for your attention