Introduction to the New Agilent 7900

Transcription

1 Introduction to the New Agilent 7900 Redefining ICP-MS Performance Spectroscopy Webinar February 2014 Presenter: Ed McCurdy ICP-MS Product Marketing, Agilent Technologies

2 Agilent 7900 ICP-MS Introduction - Agenda ICP-MS Market and Agilent Technologies ICP-MS Introduction to the new Agilent Development - Key Performance Illustrations - Unique new Hardware Features - New MassHunter 4.1 software - Support, Maintenance and User Training Q&A

3 Agilent 7900 ICP-MS Introduction - Agenda Or: What it is Where it comes from What it does How it works What it s like to use

4 Agilent s History of Innovation in ICP-MS The first 25 years to 2012 Enabling a new era in ICP-MS analysis 8800 ICP-QQQ Enabling high sensitivity metal analysis PMS series Enabling routine robust ICP-MS analysis 4500 Enabling a new level of interference management 7500 First effective He mode collision/reaction cell Enabling a new level of ease of use in ICP-MS 7700 First high-matrix (HMI) ICP-MS World s First ICP-QQQ First benchtop ICP-MS First computer-controlled ICP-MS February

5 ICP-MS Market Summary ICP-MS now a mainstream analytical technique in many regions Worldwide ICP-MS market was estimated at ~$275M in 2013 (~1700 instruments) ICP-MS has moved beyond the research lab and been adopted for routine trace metals analysis in high throughput labs For many of these labs, the key analytical requirements are: - Low detection limits (requires high sensitivity and low background) - Robustness (tolerance of complex or difficult samples) - Accuracy (freedom from interferences) - Dynamic range (ability to measure high & low concentrations in 1 run) - Productivity (sample run time; fewer sample reruns) - Ease of use (quick training of new/occasional users) - Flexibility (ability to handle a wide range of sample types)

6 So How are ICP-MS Manufacturers Responding? Current Technology to Address Routine Labs Performance Needs - Low Detection Limits Limits (requires (requires high sensitivity high sensitivity and low background) and low background) - Robustness (tolerance of complex or difficult samples) (tolerance of complex or difficult samples) - Accuracy (freedom from interferences) - Dynamic Accuracy range (freedom (ability to from measure interferences) high & low concentrations in 1 run) - Productivity Dynamic range (sample(ability run time; to fewer measure sample high reruns) & low concentrations in 1 run) - Ease Productivity of use (quick (sample training run of new/occasional time; fewer sample users) reruns) - Flexibility (ability to handle a wide range of sample types) Ease of use (quick training of new/occasional users) - Flexibility (ability to handle a wide range of sample types) Existing instruments (Agilent 7700) already addressed many of these requirements: Low Detection Limits Sufficient for ppt level analysis Accuracy He mode provides simple removal of most polyatomic overlaps in most sample types Flexibility interfaces for organic solvents, aggressive acids, HF, small samples volumes; easy coupling to alternative sample intro devices (LC, GC, FFF, Laser Ablation, etc.)

7 Agilent s ICP-MS Product Development Focus Recent ICP-MS Launches 7700 Series quadrupole ICP-MS launched 2009 Unrivalled He mode performance for multi-element interference removal Unmatched matrix tolerance with HMI (up to 2% TDS) 9 Orders dynamic range at the detector 8800 triple quadrupole ICP-MS launched 2012 Unique QQQ configuration allows operation in MS/MS mode MS/MS mode provides the only reliable way to remove interferences in complex or variable samples using reactive cell gases 8800 ICP-QQQ Won Four Industry Awards Within a Year of Launch Divergence between: High-throughput, more routine analysis (quadrupole ICP-MS with He mode) Ultra-flexible, ultra-high performance for advanced applications, semicon, materials, ultra-low DLs (ICP-QQQ in reaction mode) Let s see what else we ve been working on since the 7700 was launched

8 Why Introduce another new ICP-MS? Building on the success of the Agilent 7700 Series: 7700 had: Better matrix tolerance than any other ICP-MS More robust plasma (lower CeO/Ce ratio) than any other system under standard tuning conditions, plus HMI for routine analysis of % level dissolved solids Best interference removal with Helium cell gas eliminates need for reaction gases in all common applications 7700 ORS 3 improvements - removes all polyatomics in He mode, giving accurate results in complex or variable sample types impossible on ICP-MS systems that use reactive cell gases or mixtures Wider dynamic range than any other quadrupole ICP-MS Full 9 orders dynamic range at the detector linear to 500ppm without changing conditions or hardware

9 Areas Where Even Better Performance was Needed Focus for next generation ICP-MS after 7700 Low Detection Limits (requires high sensitivity and low background) Robustness (tolerance of complex or difficult samples) Accuracy (freedom from interferences) Dynamic range (ability to measure high & low concentrations in 1 run) Productivity (sample run time; fewer sample reruns) Ease of use (quick training of new/occasional users) Flexibility (ability to handle a wide range of sample types) Requirements to improve on existing performance and extend ICP-MS scope into new applications & sample types: Robustness Nominal limit of 0.2% (2000ppm) total dissolved solids (2% with HMI) not sufficient for routine direct analysis of some sample types Dynamic range sub-ppt DLs adequate at the low end, but over-range at a few 100ppm not sufficient for majors and traces in same run Productivity Faster analysis needed: 60 seconds per sample or less, with optimum cell gas mode for all elements Ease of use Simpler user interface, intelligent method setup and user training

10 Presenting Agilent s Game-Changing 7900 ICP-MS We took the world s bestselling, highest performing quadrupole ICP-MS, and made it 10x better!

11 Agilent 7900 ICP-MS Key Performance Gains 10x better performance than the Agilent 7700 ICP-MS 10x higher matrix tolerance handles even tougher samples than the 7700/HMI - Patented HMI is still unique to Agilent. On the Agilent 7900, the optional ultra-hmi (UHMI) extends capability to matrix levels of up to 25% TDS 10x wider dynamic range increases upper measurement limit had 9 orders (class-leading) extends this by at least an order of magnitude (up to 11 orders measurement range), allowing % levels to be quantified - a first for ICP-MS 10x better signal to noise lower Detection Limits - Higher ion transmission lens and interface, with orthogonal detector for low background Improved productivity faster analysis even when switching cell gases - New ultra fast ORS 4 with less than 3 seconds switching time between modes - New ISIS-3 for fast unattended start-up, autotune and sample delivery; full EPA-6020 analysis with 2 gas modes for optimum measurement in <1 minute per sample 30x faster detector faster transient signal measurement (TRA) - 0.1ms integration time means more flexibility in single nanoparticle analysis Easier to use new software user interface, plus advanced usability tools - Method Wizard and remote monitor/control from tablet or Smartphone

12 New 7900 ICP-MS; New Technology

13 How Much is Really New?? Easier to say what did we keep from the 7700? Components retained from 7700 Series: RF generator Sampling cone and retaining ring Quadrupole (still the only hyperbolic profile quad in ICP-MS) Agilent Mass Flow Controller (AMFC) gas control module Turbo pump That s it!! Everything else is new or re-engineered for the 7900! You may think (or be told!) that Agilent 7900 as just a facelift In fact it s almost completely new!

14 New 7900 ICP-MS Performance Highlights February

15 Illustrations of Performance Improvements Robustness (matrix tolerance) Dynamic Range Productivity - Faster discrete sampling - Faster cell gas switching Ease of Use - New software UI and features - User training; routine maintenance

16 Introducing the Ground-Breaking Agilent UHMI UHMI gas port UHMI much more than just a simple T-piece UHMI uses optimized gas mixing geometry and sophisticated plasma/gas-flow tuning algorithm to set reproducible conditions for predictable aerosol dilution rate

17 NEW 7900 Ultra High Matrix Introduction (UHMI) What s different compared to HMI on 7700 and 8800? Increased dilution range to x100 even higher matrix capability Less matrix loading to interface, so better long-term stability Maintain high carrier gas flow through spray chamber, so faster gas replacement and washout 7700 HMI New UHMI HMI-4 (HMI-L) 0.6 L/min 0.8 L/min HMI-8 (HMI-M) HMI-25 (HMI-H) HMI-50 N.A. 0.4 HMI-100 N.A UHMI Dilution Gas Port

18 Test of Real-World Matrix Tolerance with UHMI 7900 with UHMI autotuned as normal Argon gas humidifier used normal for high salt matrices Multi-element calibration in simple aqueous standards Variable NaCl matrices were then run, each spiked with multielement QC spike - Check recovery of spike level in each NaCl matrix

19 Demonstration of UHMI Performance NaCl matrix analysis with calibration against simple aqueous standards 50 Spike Recovery at 0% NaCl (first point is true spike amount) 45 0g / 100ml = 0% spike 0% 75 As [ 25 ppb ] 114 Cd [ 50 ppb ] 208 Pb [ 50 ppb ] 201 Hg [ 1 ppb ] Data supplied by Wim Proper, Eurofins Analytico, NL

20 Big Four Toxic Elements in Variable NaCl Matrices 25% is 125 times the recommended maximum for typical (non-hmi) ICP-MS 50 Spike Recovery at 0, 0.5, 1, 1.5, 2, 5, 10 and 25% NaCl g / 100ml = 25% spike 0% 0.5% 1% 1.5% 2% 5% 10% 25% 75 As [ 25 ppb ] 114 Cd [ 50 ppb ] 208 Pb [ 50 ppb ] 201 Hg [ 1 ppb ] Data supplied by Wim Proper, Eurofins Analytico, NL

21 Big Four Spiked into Different Salt Matrices NaCl Amount 75 As [ 25 ppb ] 114 Cd [ 50 ppb ] 208 Pb [ 50 ppb ] 201 Hg [ 1 ppb ] 0% % % % % % % % Average % Recovery 100% 97% 100% 93% % RSD 5% 4% 1% 6% 0g / 100ml = 0% 0.5g / 100ml = 0.5% 1g / 100ml = 1% 1.5g / 100ml = 1.5% 2g / 100ml = 2% 5g / 100ml = 5% 10g / 100ml = 10% 25g / 100ml = 25% Data supplied by Wim Proper, Eurofins Analytico, NL

22 Interfered Elements in Variable NaCl Matrices V-51 (ClO), Cr-52 (ClOH), Ni-60 (NaCl) and Cu-63 (ArNa) Spike Recovery at 0% NaCl 70 0g / 100ml = 0% spike 0% 51 V [ 50 ppb] 52 Cr [ 50 ppb ] 60 Ni [ 50 ppb ] 63 Cu [ 50 ppb ] Data supplied by Wim Proper, Eurofins Analytico, NL

23 Interfered Elements in Variable NaCl Matrices 25% is 125 times the recommended maximum for typical (non-hmi) ICP-MS Spike Recovery at 0, 0.5, 1, 1.5, 2, 5, 10 and 25% NaCl g / 100ml = 25% spike 0% 0.5% 1% 1.5% 2% 5% 10% 25% 51 V [ 50 ppb] 52 Cr [ 50 ppb ] 60 Ni [ 50 ppb ] 63 Cu [ 50 ppb ] Data supplied by Wim Proper, Eurofins Analytico, NL

24 Interfered Elements Spiked into Different Salt Matrices NaCl Amount 51 V [ 50 ppb ] 52 Cr [ 50 ppb ] 60 Ni [ 50 ppb ] 63 Cu [ 50 ppb ] 0% % % % % % % % Average % Recovery 98% 99% 98% 98% % RSD 2% 1% 3% 2% 0g / 100ml = 0% 0.5g / 100ml = 0.5% 1g / 100ml = 1% 1.5g / 100ml = 1.5% 2g / 100ml = 2% 5g / 100ml = 5% 10g / 100ml = 10% 25g / 100ml = 25% Data supplied by Wim Proper, Eurofins Analytico, NL

25 Performance Highlights Robustness (matrix tolerance) Dynamic Range Productivity - Faster discrete sampling - Faster cell gas switching Ease of Use - New software UI and features - User training; routine maintenance

26 Far Wider Measurement Range Than Any Other ICP-MS 11 orders - low and high level calibrations in a single run Cd (1ppt - 1ppb) and Na (100ppb - 10,000ppm (1%)) in the same run Both calibrations are linear. Total concentration range covered from Cd blank (BEC of <0.1ppt) to Na top standard (1%) is 11 orders Concentration range (11 orders) and upper measurement limit (>1%) are at least 10x better than any other ICP-MS

27 NEW 7900 Orthogonal Detector System Improved signal to noise Higher sensitivity High sensitivity EM (increase secondary electron generation by higher voltage at the 1st dynode) Lower background Off-axis from Q-pole to Detector Improved S/N (average 10x better than 7700 ICP-MS) Reduced noise on pulse signal New advanced discriminator system to identify and separate noise

28 Agilent 7900 Capable of Very High Signal/Noise High sensitivity combined with low background (and noise) Tuned like typical ICP-MS CeO/Ce <2.5% Uranium calibration in No Gas mode: Ultra-high sensitivity 1.38 GHz/ppm Ultra-low background (1cps): DL: 1.3ppq; BEC: 0.48ppq Importance of background in ultra-trace level measurements Note in most normal samples the background is limited by contamination Signal (Mcps/ppm) Background (cps) SBR Example Example Example

29 NEW 7900 Orthogonal Detector System Faster TRA measurement of fast transient signals Minimum dwell time for TRA acquisition is shortened to 0.1 msec on the 7900 to allow faster sampling of transient signals. This enables the measurement of the single NP peak signal arriving on the detector. 0.1 msec 30 nm Au Nanoparticle February

30 Performance Highlights Robustness (matrix tolerance) Dynamic Range Productivity - Faster discrete sampling - Faster cell gas switching Ease of Use - New software UI and features - User training; routine maintenance

31 New Integrated Sample Introduction System (ISIS 3) Fully compliant multi-mode EPA 6020 analysis* in <1 minute 7 port valve (incl. online ISTD port) Piston pump 3-way valve New features in ISIS 3 Close-coupled valve very short tube length so minimal stabilization/rinse delay Piston pump for faster sample uptake 3-way valve to switch between on-line ISTD or tune solution ISIS is now compatible with Startup auto-optimization functions and full autotune * EPA 6020 includes 23 analytes, plus 8 recommended ISTDs, so up to ~40 analytes (2 gas modes gives optimum data in terms of sensitivity and interference removal)

32 New Integrated Sample Introduction System (ISIS 3) Increase sample throughput: 30% faster 26+ elements analysis in soils, etc. (e.g. EPA 6020): 7700+ISIS 2: ~75 sec ISIS 3: <60 sec. February

33 Fast Cell Gas Switching ORS H 2 : ml/min 2 sec H 2 : ml/min 10 sec. Users confirm they can set 0 seconds stabilization time for switch between nogas, He and HE He modes

34 Performance Highlights Robustness (matrix tolerance) Dynamic Range Productivity - Faster discrete sampling - Faster cell gas switching Ease of Use - New software UI and features - User training; routine maintenance

35 MassHunter 4.1: Simpler and More Powerful Software Dashboard with Gadgets replaces old mixed UI concept Gadget icons are live ; change appearance depending on current status Also have short pull-down menus for quick access to most common functions Instrument status monitor (right) can be displayed on top of DA window, to show status and access top level functions without the need to open entire Top Level Application.

36 Software so Powerful it Can Write Your Methods! A new era in simple method setup and ease of use Method Wizard Develops a complete method in three steps! 1. Select pre-set method template and choose matrix level 2. Confirm analytes and internal standards

37 Software so Powerful it Can Write Your Methods! A new era in simple method setup and ease of use Method Wizard Develops a complete method in three steps! 3. Choose whether to optimize method for speed or DLs Select Speed or Low DL and click Optimize

38 The new batch is ready to run It can be edited or save as a template for future use Acquisition time Speed: 2.4 min Low DL: 5.7 min DL BEC unit Speed Low DL Speed Low DL Be ppt V ppt Cr ppt Co ppt Cu ppt As ppt Mo ppt Ag ppt Cd ppt Sb ppt Ba ppt Pb ppt U ppt February

39 Remote Monitor App (ios and Android devices) View instrument status and perform basic system control An Industry First! View instrument, queue and error status Ignite/extinguish plasma Pause/resume Queue

40 Performance Highlights Robustness (matrix tolerance) Dynamic Range Productivity - Faster discrete sampling - Faster cell gas switching Ease of Use - New software UI and features - User training; routine maintenance

41 Improving Installation & Familiarization Installation Intuitive Installation Checkout Site Prep tool for Software Installation Plus: Remote Advisor Now available for ICP-MS! Familiarization tools Familiarization Tutorials - Familiarization Guide - Familiarization Video - Familiarization Slide Set

42 Familiarization Tutorial Over 20 Video Clips Videos show key theory and detailed operation

43 New Maintenance Intervals Redefined based on actual 7700 user maintenance periods Item Current (7700) New (7900) Ar gas filter Replace 6 months Replace as needed Foreline Pump Oil Replace 3 months Replace 6 months Shield Plate Clean 1 month Replace as needed Extraction Lens, Omega Lens Clean 3 ~ 6 months Clean as needed Cell Entrance Lens Clean 6 months Clean as needed Plate Bias Lens Clean 6 months Clean as needed Octopole Replace 12 months Replace as needed Deflect Lens Clean 6 months Clean as needed EM Replace as needed Replace as needed Reviewed and agreed by R&D, Support, and Marketing Simpler maintenance Less downtime and cost 43

44 Summary New Agilent 7900 ICP-MS Better customer experience Better Analytical performance experience Ultra high matrix tolerance Superior sensitivity and lower background noise Wider dynamic range New Productivity Option (ISIS 3) Ultra fast scan speed for Single Nanoparticle analysis Better Software experience ICP-MS MassHunter 4.1 Method Wizard Mobile device support Better Support experience Familiarization Tutorials/Videos Remote Advisor support

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46 Q&A Questions Unanswered at Live Event Question Why is HMI any better than an online auto-dilution system? You said that triple quad was the only reliable way to measure interfered elements when using reactive cell gases. Why? How often do you calibrate the detectors? Li 6 as internal std is giving us some problems what else can we use for the low mass calibration? How long does it take for the vacuum pressure to be back up after a power interruption? Do you have any data that indicates how fast the system rinses out between two different sample types? Do you need to clean the sample interface between different sample types? Response HMI is in fact a dilution technique. The main difference is that it dilutes the sample aerosol, not the bulk liquid. This has several advantages, but the main ones are that there are no sample handling steps prior to analysis, so no contamination or dilution errors. And compared to an automatic on-line dilution system, the main benefits of HMI are the simplicity (no tubing connectors to leak or need maintenance), and the flexibility. With HMI you can run the sample once and using different tune steps you can measure it at several different dilutions, all from a single visit to the sample. it make method development really quick and easy. QQQ allows operation in MS/MS mode, which is where the first quadrupole (the one before the cell) operates as a unit mass filter. This means that only the analyte mass and any on-mass interferences enter the cell, which means the reaction chemistry is really well controlled. In practice this means that you don't get any cell-formed reaction product ions giving you new interferences on other analytes, and any analyte that you measure as a product ion itself (such as As-O at m/z 91) is not going to suffer any interferences from another analyte already at the product ion mass (such as Zr91) There are 2 sides to this question. The first is how often do you need to calibrate the detector (to ensure linearity between the pulse-count and analog ranges, so-called P/A Factor calibration). The answer to that would be about once a week if you are routinely working across both detector ranges (ie calibrating and measuring across the two ranges). But the second part is how often do users typically do the calibration in practice, and that might be much more frequently because P/A factor calibration can be set to run automatically as part of the system Startup process every time the plasma is lit. That way you can be sure that it is always calibrated and you don't need to remember to run it as a regular tuning action. The choice of a really low mass ISTD element can be quite limited because most of the elements in that mass region are either matrix elements or required analytes. However, if you optimize the instrument to give really robust operating conditions, the mass bias and ionization effects should be quite limited, and so a higher mass ISTD such as Co or Ge will still correct reasonably well for low mass analytes. The available ISTD elements will depend on your samples and your method. On the Agilent ICP-MS systems the vacuum automatically starts up again after the power is restored, so you just have to wait the normal warmup period of about 10 minutes. Even if the power is off for a long time, the vacuum in the high vacuum region only takes a few minutes to pump down. It's only a little longer (maybe 20 minutes) if the vacuum system has been opened for maintenance. The required wash out time will depend on two things 1) how different the two sample types are and 2) how low you need to measure the analytes in the second sample type. There are some obvious "worst-case" scenarios such as a lab that measures pure Co and pure Ni and needs to measure each of the matrix elements as a trace contaminant in the other. In those cases, it would take a very long time to wash out from 1000's ppm level to ppt level, and it's probably more practical to keep two sets of sample introduction and interface parts, and reserve each set for its own matrix. For other sample types, such as wastewater and clean drinking water, or clinical labs that run whole blood, urine and plasma, an extended rinse (maybe 10 minutes) followed by a few repeats of the blank for the new matrix will work fine. by any chance, do you have further data illustrating performance Hg should be a good indicator for that. hard to analyze for and the recovery at 25% NaCl is phenomenal at lower spike levels? Maybe 1ppb or lower? What IS was used for the Na study? The ISTD elements used for the NaCl tests were Li6, Sc, Ge, Rh, In and Ir. ISTD assignment was just based on mass. What solid sampling accessories are available and how do they By far the most common accessory used for solid sampling into an ICP-MS is laser ablation. This is well-established and routine (used for geochemical compare to solution operation? dating in prospecting studies, for example), and there are several well-established suppliers of laser ablation systems. It's difficult to compare solution and laser analysis because there are pros and cons for each. Sample preparation is often much simpler for direct solid samples, but sample homogeneity can be an issue. Laser gives elemental distribution information on a micron scale, but it can be much more difficult to find or make suitable standards for the analysis. Other approaches can be used for solid sampling, including ETV introduction of powders, or even slurry nebulization, but these are much less widely applicable. Will the new hardware features (ISIS 3, UHMI, new detector, There are no upgrade paths for those hardware parts for the 8800 at the moment, but of course we are always working on future product development new collision/reaction cell, ion transmission) also be available on across all our ICP-MS platforms. the 8800? How the polyatomic ions are removed in ORS in no gas mode? In no gas mode, there is not a significant reduction in polyatomic ions in the cell, although a little bit of energy discrimination can still take place even when the cell is unpressurized. But on the Agilent ICP-MS systems, we always focus a lot on making sure the plasma is tuned for very robust conditions, which means the molecular ions are being decomposed effectively in the plasma. This is monitored using the strongly-bound Ce-O molecule, so reducing the CeO level indicates that the plasma is working well to dissociate other interfering species (such as CaO, SO, SiO, etc)

47 Q&A Questions Unanswered at Live Event Question Do you have a method wizard for drinking water, can't use the ORS. Is this new software Mas Hunter 4.1. compatible with ICP-MS 7700 r s d meaning With all the capabilities of the 7900, it sounds like you don't need ICP-AES any more! Response We have pre-set methods for drinking water with He cell mode and without (so the EPA pre-set method uses only no gas mode, for example). But the Method Wizard can build a method even without an appropriate pre-set method. All you need to do is define the analyte and internal standard masses and the method optimization will still work fine. The pre-set method just saves some time by giving you a template with many parameters already predefined, but you can still edit the method to suit your specific requirements. And then you can save the modified method as a new template to be used as the basis for future methods. yes it is relative standard deviation There's certainly more overlap in capabilities now, especially for high matrix samples and high concentration analyte measurements. But it's wrong to say that there's no need for ICP-OES anymore. Two of the most important criteria in selecting an analytical instrument are often budget and fitness for purpose. Many organizations and individuals are aiming to purchase the minimum compliant solution; in other words the lowest cost instrument that is fit for purpose,. In cases where your analytical method doesn't need particularly low limits of detection, the lower cost of ICP-OES means it is often the system of choice, even if ICP-MS could also do the analysis. Many standard methods still reference ICP-OES as well, so ICP-MS cannot be used for those methods. In many laboratories, the switch from ICP-OES to ICP-MS is actually driven by the fact that the ICP-MS can do the measurements that ICP-OES can't, such as trace analytes that are currently run using GFAAS, or hydride/atomic Fluorescence for As, Se, Hg, etc). Since ICP-MS can do all those trace elements in addition to the traditional ICP-OES workload, it often makes sense to consolidate all the analyses onto a single instrument. Finally, ICP-OES precision is generally better than ICP-MS, so for high-precision major element assays, OES is still superior. Any experience (e.g. application notes) about the direct Yes, DMSO can be measured directly on the 7900 and this (and other) solvents are commonly used for sample prep for some pharmaceutical materials, detection of metal contaminants in pharmaceutical low molecular APIs etc. We have a White Paper on Pharmaceutical Analysis by ICP-MS which references some of these methods. weight compounds directly out of DMSO without further sample preparation? can the new version of Mass Hunter be applied backward to the Yes it can. There is an upgrade available which can also include the new Win 7 64bit PC, if your 7700 is currently running an earlier 32bit version of 7700X software. For single element, single particle nanoparticle studies, can you When you measure single nanoparticles, you have to monitor only a single mass, otherwise you could miss the signal for a particle being monitored at run with no quadrupole settling time? mass a while you were measuring at mass b. In this single ion monitoring time resolved analysis mode, there is no wait time or settling time between measurements. What are the possible matrix levels with out the ultra HMI option? does the new UHMI still require the argon humidifier? High energy mode - is it equal to current Normal plasma? What about Cool plasma? Thanks Does the UHMI come standard when ordering the 7900 or is it an add on? How is sensitivity affected by use of the HMI system? On the 7900, the plasma is very robust anyway (<1% CeO), and it can easily tolerate routine analysis of 0.2% total dissolved solids (TDS), even for materials that are particularly prone to deposit on the interface (so oxides of Al, Si, Ca, etc.). For relatively simple matrices, you can run level sup to 0.5%, possible a little higher if you tune for extra robustness (so low sample flow rate, lower carrier gas flow, etc.). The Ar humidifier is more related to the sample nebulization than the UHMI dilution step. At really high salt levels (and 25% NaCl is a saturated solution), when the sample reaches the nebulizer tip, the pressure drop causes the salts to crystallize out of solution. Using the Ar humidifier reduces this effect, so you don't get salt crystals building up on the nebulizer tip or devitrification of the glass. The humidifier for the 7900/UHMI is a different design from the older one, though. It is now two channel, and it uses gas permeation rather than bubbling, so the internal volume is lower and gas changes don't require such a long stabilization time. The mode we refer to as "High Energy He mode" or HE He) is a cell gas mode rather than a plasma setting. HE mode uses a higher flow of He cell gas combined with a higher cell voltage to increase the ion energies in the cell. This helps to reduce some interferences by collisional dissociation. For the plasma modes, we still have normal plasma (at various levels of robustness or CeO ratios) and cool plasma. Cool plasma requires specific sample introduction hardware and uses a different ion lens design, which can be added to the 7900 as an optional kit. UHMI is optional for the That's really only because many labs run relatively clean samples and wouldn't appreciate having to purchase the hardware that they will never need to use. HMI is a dilution system, so broadly speaking the sensitivity decreases by the UHMI dilution factor you set (100x lower sensitivity at UHMI 100x dilution, for example). Actually it's not quite as simple as this because, in a matrix, the use of UHMI reduces signal suppression, so the net signal loss is not as great as the nominal dilution factor. Also, because UHMI increases plasma robustness, it reduces suppression of the poorly ionized elements by an even greater degree, so when measured in a matrix, the relative signal drop for elements like As, Se, Cd, Hg, etc. is much less than the nominal UHMI dilution factor.

48 Q&A Questions Unanswered at Live Event Question What is the typical effect on LOD/BEC when running in UHMI mode? How much does it cost to run the instrument per sample if at full capacity for an 8 hour day? (gas cost, maintenance, etc.) How does the 7900 compare with the 8800 from the standpoint of sensitivity alone. Our most difficult matrix is CaSO4, rather than NaCl. We experience severe salt deposition on the nebulizer, with no notable improvement with the use of an argon humidifier. Has any work been done on this or other non-nacl matrices? One question: Your talked about 7900, what is 8800 set for? what is the expectations for 8800? Response For clean samples, the BEC and method DL (i.e. calculated back to the original undiluted sample) will degrade by approximately the same factor as the UHMI dilution factor (so 100x poorer for UHMI 100). However, this doesn t apply to high matrix samples, where the use of UHMI makes the plasma much more robust so signals are not suppressed as much as they are without UHMI. With UHMI, the decomposition of polyatomics is also more effective (lower CeO/Ce ratio), so some matrix interferences are lower even before the cell processes have their effect. This means that the net effect on BEC and DL is not as great as the nominal UHMI dilution factor.. The main running cost is the Argon gas, and an ICP-MS uses approximately one standard (K/L size) cylinder of Ar (10,000 liters of gas) per 8 hours of operation. Depending on the sample type and the sample introduction you are using, you should expect to get one solution analyzed (uptake, stabilization, measurement and washout) every 1 to 4 minutes, so in an 8 hour day you d get between about 100 and 400 samples measured, assuming around a 15% QC overhead. If you use the instrument a lot (or have multiple ICP instruments) the argon costs less when purchased as liquid Ar, so many labs use cryo containers or bulk liquid argon tanks. There is the cost of electricity to run the instrument (it draws around 3.5KW when the plasma is on, plus about 1.3KW for the water recirculator), but maintenance costs on a daily basis are negligible as all the main consumables last many weeks or months or more (much more in the case of costly items like the EM detector). The sensitivity of the 7900 is almost the same as the 8800, but the 8800 probably has slightly lower background. I ve only come across CaSO4 as a matrix component following its use as an extractant for soil sample analysis, but at those concentrations (I think 0.01M) it could be run routinely. Of course handling high matrix samples requires careful method development to ensure the method is compatible with the instrument hardware and operating conditions being used. Often there s a balance between modifying the instrument conditions to allow it to handle an extreme matrix (by using HMI, for example) and modifying the sample to allow it to be measured under more typical operating conditions. In the case of HMI and UHMI, we have run several other high matrix sample types, including complex salt mixes and digested metals (1% Cu solution, for example), but I don't remember seeing data on CaSO4. If you make an enquiry through your local Agilent applications person, we can investigate it. Yes, this webinar was focused on our new 7900 quadrupole ICP-MS system only, but we did several equivalent presentations when we launched the 8800 in The 8800 works well for all standard applications. However, it sets itself apart when it comes to really problematic analytes in really difficult sample types. The unique capability of the 8800 is with its MS/MS capabilities, which control the ions that enter the reaction cell, so reactive cell gases can be used selectively to remove interferences. This is different from normal quadrupole ICP-MS in reaction mode, where all the ions and matrix elements enter the cell, so the reaction processes can change completely from one matrix (or combination of analytes) to another. The 8800 can therefore use reaction chemistry to remove interferences much more effectively and reliably, which allows it to measure elements such as S and P at much lower levels than quadrupole ICP-MS, and measure interfered elements accurately at the ultra-trace levels required in semiconductor or high performance materials analysis. And unlike quadrupole ICP-MS, the 8800 can also give reliable and accurate data in reaction mode, even when the sample matrix is complex or variable. There are many examples, but if you search on the Agilent website you should be able to find a link to the 8800 Applications Handbook (publication number EN). Could you describe the unattended startup with the ISIS-3 in During the Startup process for Agilent ICP-MS systems, a series of user-selectable optimization steps can be performed automatically. So things like more detail? torch alignment, EM detector cross-calibration, lens tuning, and generating a standard system performance report can all be run automatically every time the plasma is ignited. Previously these Startup functions couldn't be used with ISIS in discrete sampling (DS) mode, because DS gives a relatively shortlived transient signal pulse from the loop injection, so the signal wasn't stable for long enough for the auto-optimization processes to be completed. The new ISIS includes a T-connector so that the tune solution can be added to the carrier continuously, in place of the ISTD solution that is normally added online. This means that Startup has a steady-state tune signal to work with so the Startup tasks can be completed just like for normal (non-ds) sample intro. Can the ECM server handle a 7700 and a 7900 simultaneously? Yes, An ECM server can handle an almost unlimited number of instruments and can even manage electronic records from non-agilent instruments. What's the long-term analyte stability (4 hours) running 25% dissolved solids using the new aerosol dilution system? We didn't have time to show that data, but it will be included in an upcoming application note, For most elements, the stability at the same spike levels we showed (50ppb for most trace elements) was between 2 and 5% RSD over the 4 hour sequence (alternating 25% NaCl and 25% NaCl plus spike).

49 Q&A Questions Unanswered at Live Event Question Does the method wizard include TRA for chromatographic analyses? Response Not at the moment, mainly because the instrument doesn t have a database of the information about all the possible sample introduction types and signal characteristics (which might be anything from monitoring a steady-state signal to a very rapidly changing transient signal). TRA acquisitions are much more difficult to define in a way that a software algorithm can work with, because of the additional variable of the signal change with time. We don t have pre-set methods for speciation analysis as yet either, although they are on the software development plan. what sample introduction and hardware has to be separated none. there is no need to switch replace or change any HW items from Sample intro. going from high matrix method to low matrix method ie. 300g/LZn matrix to low water matrix is there any changes / improvements to Semi Quant methods Do standards need to be matrix-matched when using HMI system? does the ISIS for the 7700 involve 7port valves and piston pump as well How much was the IS('s) suppressed during the Na study? Semiquant generally works very well on the 7700 already, especially in He mode, but we didn t make any specific changes to semiquant calibration in the latest revision., How that compares to what you have now will depend on which revision you are working with at the moment. No, they don't; that is one of the main benefits of the improved robustness that UHMI provides. The NaCl matrix spike recoveries (up to 25% NaCl matrix) that we showed were all measured against a calibration in simple aqueous standards (no NaCl matrix matching). No, ISIS 3 (the close-coupled-valve version with the piston pump) is currently only available for the Around 50% signal loss in 25% NaCl, and reasonably uniform suppression across the mass range. There is an App Note coming that looks into this data in a bit more detail.