Solid State NMR News. Sebastian Wegner, Bruker BioSpin GmbH, Rheinstetten, Germany 40. Deutsches NMR Meeting
|
|
|
- Blanche Adams
- 6 years ago
- Views:
Transcription
1 Solid State NMR News Sebastian Wegner, Bruker BioSpin GmbH, Rheinstetten, Germany 40. Deutsches NMR Meeting
2 Solid State NMR News Solid State DNP NMR 111 khz MAS NMR Adaptor kit for Varian and Chemagnetics probes MAS III firmware upgrades Application notes Training courses
3 Solid State DNP NMR Many applications in solid state NMR suffer from insufficient sensitivity Solid state DNP NMR today is the method of choice to solve this Biological systems (membrane proteins, fibrils etc.) Pharmaceuticals ( 13 C and 15 N 2D correlations at natural abundance Materials (frameworks, MOFs)
4 DNP of MOFs DNP-enhanced 15 N{ 1 H} CPMAS NMR spectra of UiO-66-NH 2 (a), UiO-66- NH 2 exposed to KCl (b), and Pt 2+ - loaded samples The NH 2 Pt peak grows linearlywith Pt loading of the MOF. Estimates of the ratio of free NH 2 vs. NH 2 Pt can be made based on the relative peak intensities in the NMR spectra.
5 Solid-State DNP NMR Spectrometer Gyrotron and Klystron Options DNP with Gyrotron Work in Progress: 263 GHz Klystron Offer a lower-price more compact microwave source at 263 GHz Design compatible with possible upgrade later to gyrotron for full power performance Power output ~ 5 W DNP with Klystron (EIK) Available 2017
6 111kHz - 0.7mm MAS Probe Highest available MAS rate High RF excitation in the 0.5MHz regime MAS stability of better than 0.25 at 111kHz (±28Hz max) 500nl Volume (350nl active volume) Available for SB/WB in HX / HXY / HCN configuration MAS 3 is mandatory!
7 MAS 3 High Accuracy MAS regulation for 0.7 mm Standard deviation less than 4Hz Long term stability for more than 7 hours tested
8 Tools
9 Solution State / Solid State MAS NMR
10 BL 0.7 mm first results hnh 2D 111kHz Coherence Lifetimes & Linewidths (Gb1) Data courtesy of Guido Pintacuda, ENS Lyon
11 Structure Elucidation of a capsid protein GB1 proved possibilities In 2016 unknown structures are targeted Picture shows a e.g.capsid Data on the following slides courtesy of Guido Pintacuda, ENS Lyon PNAS (33) E4757-E4758
12 1. Backbone Resonance Assignment
13 2. Elucidating >100kHz MAS 15 N-Linked Hα Detected Walk 13 CO Linked Hα Detected Walk -> linking over prolines
14 15 N-Linked Hα Detected Walk
15 3. Rapid Side Chain Assignment
16 4. Proximities Between Protons in Side-Chain
17 5. Automatic Structure Calculation with UNIO (T. Herrmann)
18
19
20 Paramagnetic Metalloproteins: Metal Ion Coordination
21 Paramagnetic Solids Large Offsets and Large Shift Anisotropies pnmr Network
22 Lanthanide luminescent complexes
23 Solid State NMR News Solid State DNP NMR 111 khz MAS NMR Adaptor kit for Varian and Chemagnetics probes MAS III firmware upgrades Application notes Training courses
24 Support of Chemagnetics, Varian and Agilent MAS probes Bruker provides an adaptor kit for solid state NMR probes from Easy integration of these probes into the Bruker workflow Full integration of the VT operation Full integration of the MAS operation Scope of the kit Probe adaptor plate VT adaptor for Johnston coupling Pneumatic adaptors and electrical interface for MAS controller
25 Support of Chemagnetics, Varian and Agilent MAS probes Probe probe adaptor plate for WB system Probe adaptor plate mounted to the probe transport frame
26 Support of Chemagnetics, Varian and Agilent MAS probes Probe with adaptor plate mounted to the Bruker Shim coil
27 Support of Chemagnetics, Varian and Agilent MAS probes Top: VT stack of a WB system with a Bruker Johnson VT adaptor for experiments above room temperature Right: poking out VT stack of WB magnet. Includes black adaptor flange on top of Bruker shim stack
28 New MAS III Firmware Different to former MAS units, MAS III firmware updates are straightforward: 1. open the Swiss FTP server ftp://ftp.bruker.ch in an internet browser 2. go to: /NMR/download/servtools/firmware/mas/ 3. download the latest firmware (e.g. to your desktop or home directory) 28
29 New MAS III Firmware Different to former MAS units, MAS III firmware updates are straightforward: 4. open TopSpin and the MAS Display ( masdisp ) 5. open the tab Configuration in the MAS display 6. Click on MAS firmware update 29
30 New MAS III Firmware Different to former MAS units, MAS III firmware updates are straightforward: 7. in the MAS webpage browse to the just downloaded firmware file 8. click on Update mas3_ _1638.bin 30
31 Application Notes 1 H-Detection for Protein Samples full set of ten 1 H-detected experiments combined with (ultra-) fast MAS for protein backbone & side chain assignment available with at least TopSpin 3.2.7/ (or on demand) 3 Application Notes explain theoretical and practical aspects: Introduction to 1 H-detection Experiments explained in more detail Straightforward optimization protocol 31
32 Application Notes BSH CP for Protein Samples Band-Selective Homonuclear Cross Polarization homonuclear C-C transfer in NCαCx and NCOCx experiments is essential for protein backbone assignment BSH CP represents a highly robust & efficient dipolar-based transfer method efficiently suppresses: unwanted sequential & long-range C-C transfers by dipolar truncation undesirable transfer to Cβ resonances five different experiments implemented from 1D up to 4D tested for protonated & deuterated samples available with at least TopSpin / or on demand 32
33 Application Notes Where to find them? Home. Products. Magnetic Resonance. NMR. Probes. Solids. Very Fast MAS. 0.7 mm. Learn More 33
34 Application Notes Where to find them? application note 34
35 New TopSolids2 Where to find Topsolids2
36 Solid State NMR Training Courses Other courses available upon request: 13 C-detected solid state NMR on protein samples 1 H-detected solid state NMR on protein samples TopSolids CRAMPS We are always open for further suggestions: solids@bruker.com 36
37 Acknowledgements core application people solid-state NMR: Gerhard Althoff (DE) Venita Decker (DE) Sebastian Wegner (DE) Barbara Perrone (CH) Jochem Struppe (US) Fabien Aussenac (F) for DNP + all the other application people world wide! important s: solids@bruker.com
38 Copyright Innovation 2015 Bruker Bruker with Corporation. Integrity Corporation. All rights reserved. All rights reserved.
Tips & Tricks around temperature
Tips & Tricks around temperature 40. NMR Benutzertagung 2016 Karlsruhe Frank Schumann Bruker BioSpin AG, Schweiz Innovation with Integrity Temperature in NMR NMR experiments are sensitive to small changes
It is possible to choose the temperature for each experiment by setting a temperature under the Temp pane (under the Standard panel).
1 2 The study queue gives a lot of flexibility for lining up experiments: they can be run at different temperatures or at different times. You must respect the instrument limits: do not submit experiments
NMR Thermometer. Variable Temperature Control Using the 2H Lock System of AVANCE III HD Spectrometers User Manual Version 002
NMR Thermometer Variable Temperature Control Using the 2H Lock System of AVANCE III HD Spectrometers User Manual Version 002 Innovation with Integrity NMR Copyright by Bruker Corporation All rights reserved.
Dipole Recoupling at High Spinning Frequencies. and. High Magnetic Fields
Dipole Recoupling at High Spinning Frequencies and High Magnetic Fields Stowe, Vermont January 2010 Francis Bitter Magnet Laboratory and Department of Chemistry Massachusetts Institute of Technology Outline
NMR FACILITY NEWSLETTER
NMR Newsletter NMR FACILITY NEWSLETTER Department of Chemistry and Biochemistry Matt Revington-Facility Coordinator mrevingt@uwindsor.ca Ext 3997 500 MHz NMR upgraded The 500 MHz NMR has received a $250,000
Pulsar. Delivering NMR to your benchtop
Pulsar NMR Delivering NMR to your benchtop Pulsar TM NMR for your laboratory The Pulsar TM NMR spectrometer from Oxford Instruments delivers affordable, high performance NMR spectroscopy into the laboratory
Supplementary Material
Supplementary Material 4D APSY-HBCB(CG)CDHD experiment for automated assignment of aromatic amino acid side chains in proteins Barbara Krähenbühl 1 Sebastian Hiller 2 Gerhard Wider 1 1 Institute of Molecular
NMR Solutions for drug discovery
NMR Solutions for drug discovery Dr. Matteo Pennestri London, UK Bruker Users Meeting Innovation with Integrity The principle of Fragment Based Screening from efficient fragments to Drug candidates Fragment
Undergraduate education with benchtop NMR
Undergraduate education with benchtop NMR Spinsolve is the perfect way to provide NMR as part of an undergraduate teaching program. The high sensitivity and advanced capability means it is fast and informative,
Intelligent NMR productivity tools
Intelligent NMR productivity tools Till Kühn VP Applications Development Pittsburgh April 2016 Innovation with Integrity A week in the life of Brian Brian Works in a hypothetical pharma company / university
Probes and Applications News. Dr. Amy Freund SMASH Torey Pines Hilton, La Jolla, CA Sunday, September 11, 2016
Probes and Applications News Dr. Amy Freund SMASH Torey Pines Hilton, La Jolla, CA Sunday, September 11, 2016 What s New & What s Cool Let s Start with Probes! CryoProbe Portfolio He cooled CryoProbes
Band-Selective Homonuclear 2D Correlation Experiments
Band-Selective Homonuclear 2D Correlation Experiments Application Note Authors Péter Sándor Agilent Technologies GmbH D76337 Waldbronn Germany Abstract This application note demonstrates the utility of
Supplementary Information Access to side- chain carbon information in deuterated solids under ultra- fast MAS through non- rotor- synchronized mixing
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry Supplementary Information Access to side- chain carbon information in deuterated solids under ultra-
Determining Chemical Structures with NMR Spectroscopy the ADEQUATEAD Experiment
Determining Chemical Structures with NMR Spectroscopy the ADEQUATEAD Experiment Application Note Author Paul A. Keifer, Ph.D. NMR Applications Scientist Agilent Technologies, Inc. Santa Clara, CA USA Abstract
Homonuclear Broadband Decoupling via PSYCHE Element Benjamin Görling, Aitor Moreno, Wolfgang Bermel
Homonuclear Broadband Decoupling via PSYCHE Element Benjamin Görling, Aitor Moreno, Wolfgang Bermel Bruker BioSpin GmbH Homonuclear broadband decoupling of proton spectra is a challenging task since standard
To Deuterate Your Proteins or Not to Deuterate? That is the Question. by Bibhuti Das, Paul Ellis, and F. David Doty
To Deuterate Your Proteins or Not to Deuterate? That is the Question. by Bibhuti Das, Paul Ellis, and F. David Doty High-resolution inverse 1 H detection in solid-state (ss) NMR spectroscopy is rapidly
BASIC NMR HANDBOOK Written by M. A. Eastman Copyright 1997, 2001, 2013, 2015, 2018
BASIC NMR HANDBOOK Written by M. A. Eastman Copyright 1997, 2001, 2013, 2015, 2018 Basic NMR Handbook Table of Contents: Preface ii viii PART 1 Chapter 1: Introduction to NMR 1 Why Study NMR? 1 The Magnetic
Protein Dynamics Relaxation techniques
Protein Dynamics Relaxation techniques Daniel Mathieu Bruker Users Meeting 2016, Karlsruhe Innovation with Integrity Proteins aren t exactly rock solid 10.11.2016 Users meeting 2016 2 Characterizing Dynamic
APPENDIX 1 Version of 8/24/05 11:01 AM Simulation of H-NMR without a structure*:
WARNING NOTICE: The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals.
Magic-Angle Spinning (MAS) drive bearing
Magic-Angle Spinning (MAS) magic-angle spinning is done pneumatically spinning frequency can be stabilized within a few Hz Magic-Angle Spinning (MAS) drive bearing Magic-Angle Spinning (MAS) Maximum spinning
Fragment-based drug discovery
Fragment-based drug discovery Dr. Till Kühn VP Applications Development MRS, Bruker BioSpion User s meeting, Brussels, November 2016 Innovation with Integrity The principle of Fragment Based Screening
Solid-state NMR and proteins : basic concepts (a pictorial introduction) Barth van Rossum,
Solid-state NMR and proteins : basic concepts (a pictorial introduction) Barth van Rossum, 16.02.2009 Solid-state and solution NMR spectroscopy have many things in common Several concepts have been/will
Tools for Structure Elucidation
Innovation with Integrity Tools for Structure Elucidation Sandra Groscurth Workflow in Natural Product Research Organism Natural Products Extraction Activity Assay Characterization of Unknown Structures
StepNOESY Overcoming Spectral Overlap in NOESY1D
StepNOESY Overcoming Spectral Overlap in NOESY1D Application Note Author David Russell NMR Applications Scientist Research Products Group Santa Clara, CA Abstract VnmrJ 3 software provides easy-to-use,
Numerical Methods for Pulse Sequence Optimisation
Numerical Methods for Pulse Sequence Optimisation Lyndon Emsley, Laboratoire de Chimie, Ecole Normale Supérieure de Lyon, & Institut Universitaire de France ECOLE NORMALE SUPERIEURE DE LYON IUF Dances
Solid-State NMR Structural Studies of Proteins Using Paramagnetic Probes
Solid-State NMR Structural Studies of Proteins Using Paramagnetic Probes Christopher Jaroniec Department of Chemistry & Biochemistry The Ohio State University Protein Structure by MAS Solid-State NMR D
Supporting information for. Towards automatic protein backbone assignment using proton-detected 4D solid-state NMR data
Supporting information for Towards automatic protein backbone assignment using proton-detected 4D solid-state NMR data Shengqi Xiang 1, Veniamin Chevelkov 1,2, Stefan Becker 1, Adam Lange 1,2,3 * 1 Max
Chemistry Department
Chemistry Department NMR/Instrumentation Facility Users Guide - VNMRJ Prepared by Leila Maurmann The following procedures should be used to acquire one-dimensional proton and carbon NMR data on the 400MHz
4 Spin-echo, Spin-echo Double Resonance (SEDOR) and Rotational-echo Double Resonance (REDOR) applied on polymer blends
4 Spin-echo, Spin-echo ouble Resonance (SEOR and Rotational-echo ouble Resonance (REOR applied on polymer blends The next logical step after analyzing and concluding upon the results of proton transversal
Overview. Magnetism. Electron paramagnetic resonance (EPR) 28/02/2014. Electron Paramagnetic Resonance and Dynamic Nuclear Polarisation AS:MIT CH916
Electron Paramagnetic Resonance and Dynamic Nuclear Polarisation AS:MIT CH916 Overview What it is Why it s useful Gavin W Morley, Department of Physics, University of Warwick Dynamic nuclear polarization
Everyday NMR. Innovation with Integrity. Why infer when you can be sure? NMR
Everyday NMR Why infer when you can be sure? Innovation with Integrity NMR Only NMR gives you definitive answers, on your terms. Over the past half-century, scientists have used nuclear magnetic resonance
National Ultrahigh-Field NMR Facility for Solids
National Ultrahigh-Field NMR Facility for Solids www.nmr900.ca Second Annual Solid-State NMR Workshop May 26, 2007, Delta Winnipeg, Albert Room Session 1 Chair I. Moudrakovski (Steacie Institute for Molecular
Date: Summer Stem Section:
Page 1 of 7 Name: Date: Summer Stem Section: Summer assignment: Build a Molecule Computer Simulation Learning Goals: 1. Students can describe the difference between a molecule name and chemical formula.
Application Note 12: Fully Automated Compound Screening and Verification Using Spinsolve and MestReNova
Application Note : Fully Automated Compound Screening and Verification Using Spinsolve and MestReNova Paul Bowyer, Magritek, Inc. and Mark Dixon, Mestrelab Sample screening to verify the identity or integrity
Christopher Pavlik Bioanalytical Chemistry March 2, 2011
Nuclear Magnetic Resonance of Proteins Christopher Pavlik Bioanalytical Chemistry March 2, 2011 Nuclear Magnetic Resonance NMR Application of a magnetic field causes absorption of EM energy that induces
NMR Spectroscopy. Guangjin Hou
NMR Spectroscopy Guangjin Hou 22-04-2009 NMR History 1 H NMR spectra of water H NMR spectra of water (First NMR Spectra on Water, 1946) 1 H NMR spectra ethanol (First bservation of the Chemical Shift,
PROTEIN NMR SPECTROSCOPY
List of Figures List of Tables xvii xxvi 1. NMR SPECTROSCOPY 1 1.1 Introduction to NMR Spectroscopy 2 1.2 One Dimensional NMR Spectroscopy 3 1.2.1 Classical Description of NMR Spectroscopy 3 1.2.2 Nuclear
NMR Spectroscopy: A Quantum Phenomena
NMR Spectroscopy: A Quantum Phenomena Pascale Legault Département de Biochimie Université de Montréal Outline 1) Energy Diagrams and Vector Diagrams 2) Simple 1D Spectra 3) Beyond Simple 1D Spectra 4)
Magnetic Resonance Spectroscopy EPR and NMR
Magnetic Resonance Spectroscopy EPR and NMR A brief review of the relevant bits of quantum mechanics 1. Electrons have spin, - rotation of the charge about its axis generates a magnetic field at each electron.
microtof-q III Innovation with Integrity The bench-mark in accurate mass LC-MS/MS ESI-Qq-TOF
microtof-q III The bench-mark in accurate mass LC-MS/MS Innovation with Integrity ESI-Qq-TOF The Advantage of Confidence in Routine The microtof-q range is widely acknowledged in setting standards in performance
Experience the most powerful benchtop NMR spectrometer
Experience the most powerful benchtop NMR spectrometer Outstanding Features Highest field strength (80 MHz) Largest chemical shift spread Highest sensitivity Superb Resolution (0.5 Hz/20 Hz) Multi-nuclear
Natural Products. Innovation with Integrity. High Performance NMR Solutions for Analysis NMR
Natural Products High Performance NMR Solutions for Analysis Innovation with Integrity NMR NMR Spectroscopy Continuous advancement in Bruker s NMR technology allows researchers to push the boundaries for
The fastest compact NMR spectrometer. spectrometer has superb sensitivity and the performance to provide useful results immediately.
Spins lvetm www..com www..com/spinsolve The fastest compact NMR spectrometer Introducing the Magritek Spinsolve NMR Spectrometer, a revolutionary analytical instrument. For the first time chemists can
NMR of large protein systems: Solid state and dynamic nuclear polarization. Sascha Lange, Leibniz-Institut für Molekulare Pharmakologie (FMP)
NMR of large protein systems: Solid state and dynamic nuclear polarization Sascha Lange, Leibniz-Institut für Molekulare Pharmakologie (FMP) The Aim of the Game solution NMR other methods solid state NMR
Interpreting and evaluating biological NMR in the literature. Worksheet 1
Interpreting and evaluating biological NMR in the literature Worksheet 1 1D NMR spectra Application of RF pulses of specified lengths and frequencies can make certain nuclei detectable We can selectively
Produktivitätswerkzeuge für die NMR
Produktivitätswerkzeuge für die NMR Till Kühn VP Applications Development Benutzertagung Karlsruhe November 2016 Innovation with Integrity A week in the life of Brian Brian Works in a hypothetical pharma
Electron Spin Resonance, Basic principle of NMR, Application of NMR in the study of Biomolecules, NMR imaging and in vivo NMR spectromicroscopy
Electron Spin Resonance, Basic principle of NMR, Application of NMR in the study of Biomolecules, NMR imaging and in vivo NMR spectromicroscopy Mitesh Shrestha Electron Spin Resonance Electron paramagnetic
Optimum levels of exchangeable protons in perdeuterated proteins for proton detection in MAS solid-state NMR spectroscopy
J Biomol NMR (2010) 46:67 73 DOI 10.1007/s10858-009-9369-0 PERSPECTIVE Optimum levels of exchangeable protons in perdeuterated proteins for proton detection in MAS solid-state NMR spectroscopy Ümit Akbey
Protein NMR. Part III. (let s start by reviewing some of the things we have learned already)
Protein NMR Part III (let s start by reviewing some of the things we have learned already) 1. Magnetization Transfer Magnetization transfer through space > NOE Magnetization transfer through bonds > J-coupling
ELECTRON PARAMAGNETIC RESONANCE
ELECTRON PARAMAGNETIC RESONANCE = MAGNETIC RESONANCE TECHNIQUE FOR STUDYING PARAMAGNETIC SYSTEMS i.e. SYSTEMS WITH AT LEAST ONE UNPAIRED ELECTRON Examples of paramagnetic systems Transition-metal complexes
Host research institute: Laboratory of G. Bodenhausen, EPFL, Lausanne (Switzerland)
Scientific report on the surface characterization of functionalized nanoparticles using DNP/NMR carried out in EPFL (Lausanne) from 29 th of April to 10 th of May 2013 Visiting scientist: Aany Sofia Lilly
ProMass Deconvolution User Training. Novatia LLC January, 2013
ProMass Deconvolution User Training Novatia LLC January, 2013 Overview General info about ProMass Features Basics of how ProMass Deconvolution works Example Spectra Manual Deconvolution with ProMass Deconvolution
e 2m e c I, (7.1) = g e β B I(I +1), (7.2) = erg/gauss. (7.3)
Chemistry 126 Molecular Spectra & Molecular Structure Week # 7 Electron Spin Resonance Spectroscopy, Supplement Like the hydrogen nucleus, an unpaired electron in a sample has a spin of I=1/2. The magnetic
Sample Shuttling Bio DNP in Liquids
EU Design Study Bio-DNP Sample Shuttling Bio DNP in Liquids Marcel Reese NMR based Structural Biology Max-Planck-Institute for Biophysical Chemistry, Göttingen Joint NMR-Life and Extend-NMR Meeting 2008,
Physical Background Of Nuclear Magnetic Resonance Spectroscopy
Physical Background Of Nuclear Magnetic Resonance Spectroscopy Michael McClellan Spring 2009 Department of Physics and Physical Oceanography University of North Carolina Wilmington What is Spectroscopy?
Quantification of Dynamics in the Solid-State
Bernd Reif Quantification of Dynamics in the Solid-State Technische Universität München Helmholtz-Zentrum München Biomolecular Solid-State NMR Winter School Stowe, VT January 0-5, 206 Motivation. Solid
Natural abundance solid-state 95 Mo MAS NMR of MoS 2 reveals precise 95 Mo anisotropic parameters from its central and satellite transitions
Electronic Supplementary Information for: Natural abundance solid-state 95 Mo MAS NMR of MoS 2 reveals precise 95 Mo anisotropic parameters from its central and satellite transitions Hans J. Jakobsen,*
NMR NEWS June To find tutorials, links and more, visit our website
Department of Chemistry NMR Facilities Director: Dr. Carlos A. Steren NMR NEWS June 2014 To find tutorials, links and more, visit our website www.chem.utk.edu/facilities/nmr Computers and software updates
Agilent s new solution for obtaining routinely quantitative results from NMR measurements. Magnetic Resonance Systems
Agilent s new solution for obtaining routinely quantitative results from NMR measurements. 1 Magnetic Resonance Systems The Scope of Analytical Chemistry Analytical Chemistry is the study of the separation,
Bruker Daltonics. EASY-nLC. Tailored HPLC for nano-lc-ms Proteomics. Nano-HPLC. think forward
Bruker Daltonics EASY-nLC Tailored HPLC for nano-lc-ms Proteomics think forward Nano-HPLC World-Class Performance with a Small Footprint Bruker Daltonics presents a nano-lc system, perfectly integrated
Quantitative Solid-State NMR Study on Ligand Surface Interaction in
Supporting Information: Quantitative Solid-State NMR Study on Ligand Surface Interaction in Cysteine-capped CdSe Magic-Sized Clusters Takuya Kurihara, Yasuto Noda,* and K. Takegoshi Division of Chemistry,
How to perform 2D NMR Experiments on the Varian/Agilent VNMRS 500 when using the Chempack interface
How to perform 2D NMR Experiments on the Varian/Agilent VNMRS 500 when using the Chempack interface 1 June 3, 2014 To start: 1. Insert your sample 2. If running any 2D spectrum or even just a 1D 13 C spectrum
NMR Predictor. Introduction
NMR Predictor This manual gives a walk-through on how to use the NMR Predictor: Introduction NMR Predictor QuickHelp NMR Predictor Overview Chemical features GUI features Usage Menu system File menu Edit
MM 2. Innovation with Integrity. The new Generation of Mobile Mass Spectrometers. Defence CBRNE
MM 2 The new Generation of Mobile Mass Spectrometers Innovation with Integrity Defence CBRNE Picture by: MOWAG GmbH Mobile CWA Detection and Identification Low weight and high performance detector Introducing
DNP enhanced frequency-selective TEDOR experiments in bacteriorhodopsin
DNP enhanced frequency-selective TEDOR experiments in bacteriorhodopsin Journal of Magnetic Resonance 202 (2010) 9-13 Bajaj S. V., Mak-Jurkauskus, A. L., Belenky, M., Herzfeld, J. and Griffin, R. MR Seminar
High-resolution probes for 19 F applications. Aitor Moreno Bruker BioSpin, Switzerland BeNeLux NMR User Meeting 2016, Brussels 25 th of November 2016
High-resolution probes for F applications Aitor Moreno Bruker BioSpin, Switzerland BeNeLux NMR User Meeting 206, Brussels 25 th of November 206 Fluorite (CaF 2 ), lat. fluores Innovation with Integrity
DIFFUSION IMAGING OF LIPID MEMBRANES: DEVELOPING MULTI-PULSE NMR TO IMAGE STATIC MEMBRANE SAMPLES. Kevin Thomas Bivona
DIFFUSION IMAGING OF LIPID MEMBRANES: DEVELOPING MULTI-PULSE NMR TO IMAGE STATIC MEMBRANE SAMPLES by Kevin Thomas Bivona Bachelor of Science, University of Tennessee at Chattanooga, 2009 submitted to the
SUPPORTING INFORMATION. for. Investigations of dynamic amyloid-like structures of the Wnt signalling pathway by solid-state NMR
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2018 SUPPORTING INFORMATION for Investigations of dynamic amyloid-like structures of the Wnt signalling
Polarised Nucleon Targets for Europe, 2nd meeting, Bochum 2005
Polarised Nucleon Targets for Europe, nd meeting, Bochum Temperature dependence of nuclear spin-lattice relaxations in liquid ethanol with dissolved TEMPO radicals H. Štěpánková, J. Englich, J. Kohout,
Principios Básicos de RMN en sólidos destinado a usuarios. Gustavo Monti. Fa.M.A.F. Universidad Nacional de Córdoba Argentina
Principios Básicos de RMN en sólidos destinado a usuarios Gustavo Monti Fa.M.A.F. Universidad Nacional de Córdoba Argentina CONTENIDOS MODULO 2: Alta resolución en sólidos para espines 1/2 Introducción
NMR NEWS October 2009
Department of Chemistry NMR Facilities Director: Dr. Carlos A. Steren NMR NEWS October 2009 To check on the instrumental status and reservation system, and find tutorials, links and more, visit our website
ESI for. A rotaxane host system containing integrated triazole C H hydrogen bond donors for anion recognition. Nicholas G. White & Paul D.
ESI for A rotaxane host system containing integrated triazole C H hydrogen bond donors for anion recognition Nicholas G. White & Paul D. Beer* Contents 1 Details of instrumentation 2 NMR Spectra of new
North Carolina State University Department of Chemistry Varian NMR Training Manual
North Carolina State University Department of Chemistry Varian NMR Training Manual by J.B. Clark IV & Dr. S. Sankar 1 st Edition 05/15/2009 Section 3: Glide Program Operations for Advanced 1D & 2D Spectra
Supporting information for
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2016 Supporting information for Backbone assignment for minimal protein amounts of low structural homogeneity
NMR parameters intensity chemical shift coupling constants 1D 1 H spectra of nucleic acids and proteins
Lecture #2 M230 NMR parameters intensity chemical shift coupling constants Juli Feigon 1D 1 H spectra of nucleic acids and proteins NMR Parameters A. Intensity (area) 1D NMR spectrum: integrated intensity
Magnetic Resonance Spectroscopy
INTRODUCTION TO Magnetic Resonance Spectroscopy ESR, NMR, NQR D. N. SATHYANARAYANA Formerly, Chairman Department of Inorganic and Physical Chemistry Indian Institute of Science, Bangalore % I.K. International
Magnetic Resonance Lectures for Chem 341 James Aramini, PhD. CABM 014A
Magnetic Resonance Lectures for Chem 341 James Aramini, PhD. CABM 014A jma@cabm.rutgers.edu " J.A. 12/11/13 Dec. 4 Dec. 9 Dec. 11" " Outline" " 1. Introduction / Spectroscopy Overview 2. NMR Spectroscopy
Magnetic Resonance in Quantum Information
Magnetic Resonance in Quantum Information Christian Degen Spin Physics and Imaging group Laboratory for Solid State Physics www.spin.ethz.ch Content Features of (nuclear) magnetic resonance Brief History
Esri Defense Mapping: Cartographic Production. Bo King
Esri Defense Mapping: Cartographic Production Bo King Agenda Esri Defense Mapping: Cartographic Production Solution Overview Desktop Cartographic Production Workflow - Create Rapid Graphic Product On Demand
Concepts on protein triple resonance experiments
2005 NMR User Training Course National Program for Genomic Medicine igh-field NMR Core Facility, The Genomic Research Center, Academia Sinica 03/30/2005 Course andout Concepts on protein triple resonance
CHEM / BCMB 4190/6190/8189. Introductory NMR. Lecture 10
CHEM / BCMB 490/690/889 Introductory NMR Lecture 0 - - CHEM 490/690 Spin-Echo The spin-echo pulse sequence: 90 - τ - 80 - τ(echo) Spins echoes are widely used as part of larger pulse sequence to refocus
Welcome to: Bruker User s Meeting at PANIC
Welcome to: Bruker User s Meeting at PANIC Kim Colson March 4, 2018 La Jolla, CA, USA March 6, 2018 Expanding NMR s Presence 2 NEWS: Opening Bruker s New Center of Excellence, February 2017 in Beijing,
13/02/2017. Overview. Magnetism. Electron paramagnetic resonance (EPR) Electron Paramagnetic Resonance and Dynamic Nuclear Polarisation CH916
Electron Paramagnetic Resonance and Dynamic Nuclear Polarisation CH916 Overview What it is Why it s useful Gavin W Morley, Department of Physics, University of Warwick Dynamic nuclear polarization Why
Chapter 7. Nuclear Magnetic Resonance Spectroscopy
Chapter 7 Nuclear Magnetic Resonance Spectroscopy I. Introduction 1924, W. Pauli proposed that certain atomic nuclei have spin and magnetic moment and exposure to magnetic field would lead to energy level
Triple Resonance Experiments For Proteins
Triple Resonance Experiments For Proteins Limitations of homonuclear ( 1 H) experiments for proteins -the utility of homonuclear methods drops quickly with mass (~10 kda) -severe spectral degeneracy -decreased
Biochemistry 530 NMR Theory and Practice
Biochemistry 530 NMR Theory and Practice Gabriele Varani Department of Biochemistry and Department of Chemistry University of Washington Lecturer: Gabriele Varani Biochemistry and Chemistry Room J479 and
10.4 Continuous Wave NMR Instrumentation
10.4 Continuous Wave NMR Instrumentation coherent detection bulk magnetization the rotating frame, and effective magnetic field generating a rotating frame, and precession in the laboratory frame spin-lattice
NMR FACILITY User's Guide
NMR FACILITY User's Guide Texas A&M University Department of Chemistry August 19, 2010 Table of Contents I. Introduction...5 II. Experiments Available...5 A. One Dimensional NMR...5 1. 'Normal' spectra...5
Laserunterstützte magnetische Resonanz
Laserunterstützte magnetische Resonanz http://e3.physik.uni-dortmund.de Dieter Suter Magnetische Resonanz Prinzip Die MR mißt Übergänge zwischen unterschiedlichen Spin-Zuständen. Diese werden durch ein
- Basic understandings: - Mapping interactions:
NMR-lecture April 6th, 2009, FMP Berlin Outline: Christian Freund - Basic understandings: Relaxation Chemical exchange - Mapping interactions: -Chemical shift mapping (fast exchange) Linewidth analysis
Lecture 02 Nuclear Magnetic Resonance Spectroscopy Principle and Application in Structure Elucidation
Application of Spectroscopic Methods in Molecular Structure Determination Prof. S. Sankararaman Department of Chemistry Indian Institution of Technology Madras Lecture 02 Nuclear Magnetic Resonance Spectroscopy
Simulation of Second Order Spectra Using SpinWorks. CHEM/BCMB 8190 Biomolecular NMR UGA, Spring, 2005
Simulation of Second Order Spectra Using SpinWorks CHEM/BCMB 8190 Biomolecular NMR UGA, Spring, 2005 Introduction Although we frequently assume that scalar couplings are small compared to the differences
DRUG DISCOVERY TODAY ELN ELN. Chemistry. Biology. Known ligands. DBs. Generate chemistry ideas. Check chemical feasibility In-house.
DRUG DISCOVERY TODAY Known ligands Chemistry ELN DBs Knowledge survey Therapeutic target Generate chemistry ideas Check chemical feasibility In-house Analyze SAR Synthesize or buy Report Test Journals
Spectrum simulation using the Bruker program Simfonia
Spectrum simulation using the Bruker program Simfonia This is the start screen: To load an experimental spectrum, click on : and it will enable simulation of fluid solution (i.e. isotropic) spectra.
Biochemistry 530 NMR Theory and Practice. Gabriele Varani Department of Biochemistry and Department of Chemistry University of Washington
Biochemistry 530 NMR Theory and Practice Gabriele Varani Department of Biochemistry and Department of Chemistry University of Washington 1D spectra contain structural information.. but is hard to extract:
16.1 Introduction to NMR Spectroscopy. Spectroscopy. Spectroscopy. Spectroscopy. Spectroscopy. Spectroscopy 4/11/2013
What is spectroscopy? NUCLEAR MAGNETIC RESONANCE (NMR) spectroscopy may be the most powerful method of gaining structural information about organic compounds. NMR involves an interaction between electromagnetic
Laboration 8a. Relaxation, T 1 -measurement with inversion recovery
, T 1 -measurement with inversion recovery KR Theory The way the magnetizations returns to equilibrium, relaxation, is a very important concept in NMR, for example, due to the fact that the rate of relaxation
Electronic Supporting information for:
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 204 Electronic Supporting information for: Dynamic nuclear polarization NMR of nanosized zirconium phosphate
Advanced Quadrupolar NMR. Sharon Ashbrook School of Chemistry, University of St Andrews
Advanced Quadrupolar NMR Sharon Ashbrook School of Chemistry, University of St Andrews Quadrupolar nuclei: revision single crystal powder ST 500 khz ST ω 0 MAS 1 khz 5 khz second-order broadening Example:
Department of Physics (857) University of California, Santa Barbara, Santa Barbara, CA
Christopher Blake Wilson Curriculum vitae Department of Physics (857) 209-4798 University of California bwil@physics.ucsb.edu Santa Barbara, CA 93106 Education University of California, Santa Barbara,