Identification of Stomatopod Larvae In Banten Bay, Indonesia Using DNA Barcoding

Size: px
Start display at page:

Download "Identification of Stomatopod Larvae In Banten Bay, Indonesia Using DNA Barcoding"

Transcription

1 Identification of Stomatopod Larvae In Banten Bay, Indonesia Using DNA Barcoding Mariana D. Bayu Intan, M.Si. Dr. Abinawanto Drs. Wisnu Wardhana, M.Si. International Seminar on Marine and Fisheries Product Processing and Biotechnology September 26, 2014

2 Introduction Praying mantis-like raptorial claws Lobster-like crustaceans

3 Introduction Distribution Map of Stomatopod 115 species (23,95%)

4 Introduction Economic Value Oratosquilla oratoria Hong kong Kuala Tungkal, Jambi Harpiosquilla raphidea Harpiosquilla harpax Taiwan Tang 2009; Wardiatno & Mashar 2011; Mulyono dkk. 2013

5 Introduction Ecological Value One of meroplankton community Indicator of climate-induced changes in temperature Kirby dkk Abundance of pelagic fish (Skipjack tuna, grouper, and snapper fish) Bullard dkk. 1999; Ahyong & Harling 2000; Kirby dkk. 2008; Tang dkk. 2010

6 Introduction Indonesia P. Jawa Diversity of meroplankton Incl. Stomatopod larvae 2% 4% 2% 91% Ikan Demersal karang and dan pelagic pelagisfish Udang Shrimp Kepiting Crap Kerang Shellfish Cumi-cumi Squid Maintaining the sum of pelagic fish

7 Introduction 115 species of stomatopod Hutomo & Moosa spesies 72,17% 32 spesies 27,82% Spesies sequences found in NCBI Alima pacifica Gonodactylus smithii Chorisquilla excavata Haptosquilla glyptocercus Chorisquilla hystrix Haptosquilla pulchella Echinosquilla guerinii Haptosquilla stoliura Erugosquilla woodmasoni Haptosquilla trispinosa Fallosquilla fallax Harpiosquilla harpax Gonodactylaceus falcatus Harpiosquilla raphidea Gonodactylaceus glabrous Hoplosquilla said Gonodactylellus affinis Lysiosquillina maculata Gonodactylellus annularis Miyakea nepa Gonodactylellus caldwelli Odontodactylus latirostris Gonodactylellus erdmanni Odontodactylus scyllarus Gonodactylellus espinosus Pseudosquilla ciliata Gonodactylinus viridis Pseudosquillana richeri Gonodactylus childi Raoulserenea oxyrhyncha Gonodactylus platysoma Taku spinosocarinatus Source: National Center for Biotechnology Information (NCBI) 2013

8 Introduction DNA barcoding New method in taxonomic research which aimed to identify organisms in species-level by comparing the sequences divergence within taxa. Hajibabaei dkk. 2007; Tang dkk DNA barcoding Cytochrome c oxidase subunit 1 (COI) Animals spesies Plants 520 spesies Protist spesies Fungy 410 spesies Research of DNA barcoding in Stomatopod larvae Barber & Boyce 2006 Stomatopod larvae Lui dkk Oratosquilla oratoria adults Tang dkk Stomatopod larvae

9 Aim Identify and give more understanding about the diversity of stomatopod larvae in Banten Bay, Indonesia using DNA barcoding. Assess the effectiveness of DNA barcoding application in identifying stomatopod larvae in Banten Bay. Complete database of COI sequence in Gene Bank.

10 Material and Methods

11 Material and Methods Research Location Banten Bay Samples collection October-November 2013 Morphotype identification and molecular analysis Lab. Genetic, Departement of Biologi, FMIPA, UI

12 Material and Methods Samples collection 10 minutes Kec. 1-2 knot (1,87-3,74 km/jam) ❷ ❶ Samples collection Formalin 4% (10 drops) ❶ ❷ Samples stored in cool box

13 Material and Methods Morphotype identification 40x magnification Carapace Median spine Ventrolateral spine

14 Material and Methods Molecular analysis Extracted with kit DNA extraction COI amplification Reagent Volume (µl) Profil PCR: Destiled water PCR Ready mix LCO 1490 HCO 2198 DNA template 18,5 25 1,25 1,25 8 Total C 95 C 03:00 00:35 45 C 00:30 35x 72 C 00:50 72 C 07:00 4 C Sequencing

15 Material and Methods Molecular analysis Software ❶ Chromas Lite & BioEdit v7.0.9 Evaluating and editing electropherogram ❷ MEGA 6 Multi-sequence alignments and construct phylogeny tree

16 Result and Discussion

17 Result and Discussion Microscopic morphotype identification Morphotype n Morphotype n Morphotype n Morphotype n Sto 1 2 Sto 4 3 Sto 7 4 Sto 10 7 Sto 2 3 Sto 5 1 Sto 8 2 Sto 3 3 Sto 6 8 Sto 9 4 n Total 37

18 Percentage (%) Result and Discussion Effectiveness of DNA barcoding COI Percentation of identified stomatpod larvae MOTU MOTU 1 MOTU 2 MOTU 3 MOTU 4 MOTU 5 *Molecular operational taxonomic units ,95% Species level Tingkat spesies 54,05 % Family level Tingkat famili Species Anchisquilla fasciata Famili Squillidae Stomatopoda sp. 1 RWKT-2009 Famili Squillidae Stomatopoda sp. PHB-2006 morphotype E

19 Protosquillidae Squillidae

20 Protosquillidae Squillidae

21 Result and Discussion Effectiveness of DNA barcoding COI Species range of minimum K2P variation among clades was less than 3% Barber & Boyce 2006 Morphotype MOTU Maximum divergence within clades (%) Minimum divergence among clades (%) Closest sister group Sto 1 Sto 2 MOTU 1 1,1 0,4 Anchisquilla fasciata Sto 3 Sto 4 MOTU 2 0,9 8,9 Clorida decorata Sto 5 MOTU 3 n.a 0,5 Stomatopoda sp. 1 RWKT-2009 Sto 6 Sto 7 Sto 8 Sto 9 Sto 10 MOTU 4 0,7 5,2 Stomatopoda sp. 2 RWKT-2009 MOTU 5 2,4 1,5 Stomatopoda sp. PHB-2006 morphotype E

22 Result and Discussion Effectiveness of DNA barcoding COI Relative percentage (%) Effectiveness of barcoding gap between K2P intra- and DNA barcoding interspesific variation COI Meyer & Paulay 2005 Effectiveness of DNA barcoding Cytochrome c oxidase sub-unit 1 (COI) barcoding gap Intraspesies Interspesies Sequence variation (%) Intraspecies Interspecies Frequency distributions of genetic variability (Kimura 2-Parameter distances) (37 individuals in 5 MOTU)

23 Result and Discussion Diversity of Stomatopod in Banten Bay No. Species Family 1. Anchisquilla fasciata Squillidae 2. Unidentified sp. 1 BTN-2013 Squillidae 3. Stomatopoda sp. 1 RWKT-2009 Squillidae 4. Unidentified sp. 2 BTN-2013 Squillidae 5. Stomatopoda sp. PHB-2006 morphotype E Squillidae Mulyono Harpiosquilla harpax Squillidae 2. Harpiosquilla raphidea Squillidae 3. Miyakea nepa Squillidae

24 Conclusion ❶ DNA barcoding COI effective to identified stomatopod larvae in Banten Bay with variation of variation of Kimura-2 parameter distance ranged between 0,7% to 2,4%. ❷ There were three species of stomatopod larvae (Anchisquilla fasciata, Stomatopoda sp. 1 RWKT-2009 and Stomatopoda sp. PHB-2006 morphotype E) and 2 unidentified Squillidea species of stomatopod larvae (MOTU 2 and 4) were found in this study.

25 Thank You

LEVERS AND LINKAGES: MECHANICAL TRADE-OFFS IN A POWER-AMPLIFIED SYSTEM

LEVERS AND LINKAGES: MECHANICAL TRADE-OFFS IN A POWER-AMPLIFIED SYSTEM ORIGINAL ARTICLE doi:10.1111/evo.12407 LEVERS AND LINKAGES: MECHANICAL TRADE-OFFS IN A POWER-AMPLIFIED SYSTEM Philip S. L. Anderson, 1,2 Thomas Claverie, 3,4 and S. N. Patek 1 1 Department of Biology,

More information

Sudiarsa. AACL Bioflux, 2018, Volume 11, Issue

Sudiarsa. AACL Bioflux, 2018, Volume 11, Issue Morphometric and genetic diversity of mantis shrimp Harpiosquilla raphidea from Karimata strait and Java Sea waters, Indonesia 1 Mugi Mulyono, 2 Abinawanto, 1 Mardiyono, 1 Muhamad Y. Syam, 3 I N. Sudiarsa

More information

DNA Barcoding Fishery Resources:

DNA Barcoding Fishery Resources: DNA Barcoding Fishery Resources: A case study in Shandong Costal Water Shufang Liu Laboratory of Molecular ecology of fishery resources Yellow Sea Fisheries Research Institute (YSFRI) Chinese Academy of

More information

LUSITA MEILANA 1 *, Yusli Wardiatno 1, Nurlisa A Butet 1, Majariana Krisanti 1, Hefni Effendi 2

LUSITA MEILANA 1 *, Yusli Wardiatno 1, Nurlisa A Butet 1, Majariana Krisanti 1, Hefni Effendi 2 Assessment of The Phylogenetic Relationship among Indonesian Horseshoe Crabs Collected from Java Island Using mtdna COI Gene in Combination with Morphological Characters LUSITA MEILANA 1 *, Yusli Wardiatno

More information

Amy Driskell. Laboratories of Analytical Biology National Museum of Natural History Smithsonian Institution, Wash. DC

Amy Driskell. Laboratories of Analytical Biology National Museum of Natural History Smithsonian Institution, Wash. DC DNA Barcoding Amy Driskell Laboratories of Analytical Biology National Museum of Natural History Smithsonian Institution, Wash. DC 1 Outline 1. Barcoding in general 2. Uses & Examples 3. Barcoding Bocas

More information

A reprint from American Scientist

A reprint from American Scientist A reprint from American Scientist the magazine of Sigma Xi, The Scientific Research Society This reprint is provided for personal and noncommercial use. For any other use, please send a request to Permissions,

More information

DNA Barcoding and taxonomy of Glossina

DNA Barcoding and taxonomy of Glossina DNA Barcoding and taxonomy of Glossina Dan Masiga Molecular Biology and Biotechnology Department, icipe & Johnson Ouma Trypanosomiasis Research Centre, KARI The taxonomic problem Following ~250 years of

More information

Censusing the Sea in the 21 st Century

Censusing the Sea in the 21 st Century Censusing the Sea in the 21 st Century Nancy Knowlton & Matthieu Leray Photo: Ove Hoegh-Guldberg Smithsonian s National Museum of Natural History Estimates of Marine/Reef Species Numbers (Millions) Marine

More information

RESEARCH ARTICLE Comparative spring mechanics in mantis shrimp

RESEARCH ARTICLE Comparative spring mechanics in mantis shrimp 1317 The Journal of Experimental Biology 216, 1317-1329 213. Published by The Company of Biologists Ltd doi:1.1242/jeb.78998 RESEARCH ARTICLE Comparative spring mechanics in mantis shrimp S. N. Patek 1,

More information

Stomatopod Crustacea Collected by the Galathea Expedition, , with a List of Stomatopoda Known from Depths below 400 Meters

Stomatopod Crustacea Collected by the Galathea Expedition, , with a List of Stomatopoda Known from Depths below 400 Meters Stomatopod Crustacea Collected by the Galathea Expedition, 1950-1952, with a List of Stomatopoda Known from Depths below 400 Meters RAYMOND B. MANNING I W 9\ SMITHSONIAN CONTRIBUTIONS TO ZOOLOGY NUMBER

More information

Evolutionary variation in the expression of phenotypically. plastic color vision in Caribbean mantis shrimps, genus Neogonodactylus.

Evolutionary variation in the expression of phenotypically. plastic color vision in Caribbean mantis shrimps, genus Neogonodactylus. Evolutionary variation in the expression of phenotypically plastic color vision in Caribbean mantis shrimps, genus Neogonodactylus. ALEXANDER G. CHEROSKE 1 *, PAUL H. BARBER 2, & THOMAS W. CRONIN 1 1 Department

More information

Diversity and Functions of Protozoa in Soils

Diversity and Functions of Protozoa in Soils Diversity and Functions of Protozoa in Soils EcoFINDERS University of Cologne - AG Bonkowski 05 October 2011 Outlook Interaction studies Identification Diversity of Protozoa in Soils Single-celled, heterotrophic

More information

Species Identification and Barcoding. Brendan Reid Wildlife Conservation Genetics February 9th, 2010

Species Identification and Barcoding. Brendan Reid Wildlife Conservation Genetics February 9th, 2010 Species Identification and Barcoding Brendan Reid Wildlife Conservation Genetics February 9th, 2010 Why do we need a genetic method of species identification? Black-knobbed Map Turtle (Graptemys nigrinoda)

More information

DNA metabarcoding reveals diverse but selective diet of three-spined stickleback in a coastal ecosystem

DNA metabarcoding reveals diverse but selective diet of three-spined stickleback in a coastal ecosystem DNA metabarcoding reveals diverse but selective diet of three-spined stickleback in a coastal ecosystem Jakubavičiūtė E* 1, Bergström U 2, Haenel Q 3, Bourlat SJ 4, Eklöf J 5 1 Nature Research Centre,

More information

SCIENTIFIC EVIDENCE TO SUPPORT THE THEORY OF EVOLUTION. Using Anatomy, Embryology, Biochemistry, and Paleontology

SCIENTIFIC EVIDENCE TO SUPPORT THE THEORY OF EVOLUTION. Using Anatomy, Embryology, Biochemistry, and Paleontology SCIENTIFIC EVIDENCE TO SUPPORT THE THEORY OF EVOLUTION Using Anatomy, Embryology, Biochemistry, and Paleontology Scientific Fields Different fields of science have contributed evidence for the theory of

More information

Microbiome: 16S rrna Sequencing 3/30/2018

Microbiome: 16S rrna Sequencing 3/30/2018 Microbiome: 16S rrna Sequencing 3/30/2018 Skills from Previous Lectures Central Dogma of Biology Lecture 3: Genetics and Genomics Lecture 4: Microarrays Lecture 12: ChIP-Seq Phylogenetics Lecture 13: Phylogenetics

More information

Plant Names and Classification

Plant Names and Classification Plant Names and Classification Science of Taxonomy Identification (necessary!!) Classification (order out of chaos!) Nomenclature (why not use common names?) Reasons NOT to use common names Theophrastus

More information

BINF6201/8201. Molecular phylogenetic methods

BINF6201/8201. Molecular phylogenetic methods BINF60/80 Molecular phylogenetic methods 0-7-06 Phylogenetics Ø According to the evolutionary theory, all life forms on this planet are related to one another by descent. Ø Traditionally, phylogenetics

More information

BIOLOGY IB DIPLOMA PROGRAM PARENTS ORIENTATION DAY

BIOLOGY IB DIPLOMA PROGRAM PARENTS ORIENTATION DAY BIOLOGY IB DIPLOMA PROGRAM PARENTS ORIENTATION DAY 2018-2019 ADITYA NUGRAHA WARDHANA EDUCATION BACKGROUND: Bachelor of Science, Biology major University of Indonesia, Depok WORK EXPERIENCE: Laboratory

More information

Investigation 3: Comparing DNA Sequences to Understand Evolutionary Relationships with BLAST

Investigation 3: Comparing DNA Sequences to Understand Evolutionary Relationships with BLAST Investigation 3: Comparing DNA Sequences to Understand Evolutionary Relationships with BLAST Introduction Bioinformatics is a powerful tool which can be used to determine evolutionary relationships and

More information

STOMATOPOD CRUSTACEA FROM ANAMBAS AND NATUNA ISLANDS, SOUTH CHINA SEA, INDONESIA

STOMATOPOD CRUSTACEA FROM ANAMBAS AND NATUNA ISLANDS, SOUTH CHINA SEA, INDONESIA THE RAFFLES BULLETIN OF ZOOLOGY 2004 THE RAFFLES BULLETIN OF ZOOLOGY 2004 Supplement No. 11: 61-66 STOMATOPOD CRUSTACEA FROM ANAMBAS AND NATUNA ISLANDS, SOUTH CHINA SEA, INDONESIA Shane. T. Ahyong Department

More information

DNA BARCODING FRESHWATER ROTIFERA OF MEXICO

DNA BARCODING FRESHWATER ROTIFERA OF MEXICO DNA BARCODING FRESHWATER ROTIFERA OF MEXICO Alma Estrella García Morales Manuel Elías Gutiérrez Zooplankton Laboratory El Colegio de la Frontera Sur, Chetumal, Mexico INTRODUCTION Systematics in Rotifera

More information

Cladistics and Bioinformatics Questions 2013

Cladistics and Bioinformatics Questions 2013 AP Biology Name Cladistics and Bioinformatics Questions 2013 1. The following table shows the percentage similarity in sequences of nucleotides from a homologous gene derived from five different species

More information

University of Bristol - Explore Bristol Research. Peer reviewed version. Link to published version (if available): /jeb.

University of Bristol - Explore Bristol Research. Peer reviewed version. Link to published version (if available): /jeb. How, M. J., Porter, M., Radford, A. N., Feller, K., Temple, S. E., R.L., C.,... Roberts, N. W. (2014). Out of the blue: the evolution of horizontally polarized signals in Haptosquilla (Crustacea, Stomatopoda,

More information

Eric D. Stein Biology Department

Eric D. Stein Biology Department Eric D. Stein Biology Department The Promise of Molecular Methods Faster answers Weeks vs. months Less expensive Better data Recognizing misidentifications Improving taxonomic keys Helping with difficult

More information

BIOL 101 Introduction to Biological Research Techniques I

BIOL 101 Introduction to Biological Research Techniques I BIOL 101 Introduction to Biological Research Techniques I 1. Develop a research plan including hypothesis, controls and procedures. 2. Conduct a primary literature review relating to their research project.

More information

Microbial Diversity and Assessment (II) Spring, 2007 Guangyi Wang, Ph.D. POST103B

Microbial Diversity and Assessment (II) Spring, 2007 Guangyi Wang, Ph.D. POST103B Microbial Diversity and Assessment (II) Spring, 007 Guangyi Wang, Ph.D. POST03B guangyi@hawaii.edu http://www.soest.hawaii.edu/marinefungi/ocn403webpage.htm General introduction and overview Taxonomy [Greek

More information

SPECIATION. REPRODUCTIVE BARRIERS PREZYGOTIC: Barriers that prevent fertilization. Habitat isolation Populations can t get together

SPECIATION. REPRODUCTIVE BARRIERS PREZYGOTIC: Barriers that prevent fertilization. Habitat isolation Populations can t get together SPECIATION Origin of new species=speciation -Process by which one species splits into two or more species, accounts for both the unity and diversity of life SPECIES BIOLOGICAL CONCEPT Population or groups

More information

OCR (A) Biology A-level

OCR (A) Biology A-level OCR (A) Biology A-level Topic 4.2: Biodiversity Notes Biodiversity is the variety of living organisms, over time the variety of life on Earth has become more extensive but now it is being threatened by

More information

Students in AP Biology meet 5 days a week for 36 weeks. Periods are 56 minutes long.

Students in AP Biology meet 5 days a week for 36 weeks. Periods are 56 minutes long. Course Overview Students in AP Biology meet 5 days a week for 36 weeks. Periods are 56 minutes long. In general, the course is divided into three teaching components: laboratory, lecture, and discussion.

More information

Building the Tree of Life

Building the Tree of Life Building the Tree of Life THINK ABOUT IT The process of identifying and naming all known organisms, living and extinct, is a huge first step toward the goal of systematics. Yet naming organisms is only

More information

DRMREEF: DNA taxonomy and Recruitment Monitoring of the Coral Reef Marine Organisms

DRMREEF: DNA taxonomy and Recruitment Monitoring of the Coral Reef Marine Organisms DRMREEF: DNA taxonomy and Recruitment Monitoring of the Coral Reef Marine Organisms 2015. 05. 13 Youn-Ho Lee Korea Institute of Ocean Science and Technology I. Objectives To enhance national and regional

More information

Phylogenetic diversity and conservation

Phylogenetic diversity and conservation Phylogenetic diversity and conservation Dan Faith The Australian Museum Applied ecology and human dimensions in biological conservation Biota Program/ FAPESP Nov. 9-10, 2009 BioGENESIS Providing an evolutionary

More information

Genus Harpiosquilla r (Crustacea, Stomatopoda),

Genus Harpiosquilla r (Crustacea, Stomatopoda), RAYMOND B. MANNING A Review of the Genus Harpiosquilla r (Crustacea, Stomatopoda), r with Descriptions of Three Mew Species SMITHSONIAN CONTRIBUTIONS TO ZOOLOGY 1969 NUMBER 36 SERIAL PUBLICATIONS OF THE

More information

Chapter 19: Taxonomy, Systematics, and Phylogeny

Chapter 19: Taxonomy, Systematics, and Phylogeny Chapter 19: Taxonomy, Systematics, and Phylogeny AP Curriculum Alignment Chapter 19 expands on the topics of phylogenies and cladograms, which are important to Big Idea 1. In order for students to understand

More information

Modern Evolutionary Classification. Section 18-2 pgs

Modern Evolutionary Classification. Section 18-2 pgs Modern Evolutionary Classification Section 18-2 pgs 451-455 Modern Evolutionary Classification In a sense, organisms determine who belongs to their species by choosing with whom they will mate. Taxonomic

More information

AP Biology. Read college-level text for understanding and be able to summarize main concepts

AP Biology. Read college-level text for understanding and be able to summarize main concepts St. Mary's College AP Biology Continuity and Change Consider how specific changes to an ecosystem (geological, climatic, introduction of new organisms, etc.) can affect the organisms that live within it.

More information

ASEAN Trans-Boundary Marine Debris and Consortium for Indonesia Oceanic Research

ASEAN Trans-Boundary Marine Debris and Consortium for Indonesia Oceanic Research ASEAN Trans-Boundary Marine Debris and Consortium for Indonesia Oceanic Research Dr. Mutiara Rachmat Putri Research Group of Oceanography Institut Teknologi Bandung, INDONESIA Regional Workshop in support

More information

Connectivity using DNA the basics

Connectivity using DNA the basics Connectivity using DNA the basics Greg Rouse Scripps Institution of Oceanography WORKSHOP ON THE DESIGN OF IMPACT REFERENCE ZONES AND PRESERVATION REFERENCE ZONES IN DEEP-SEA MINING CONTRACT AREAS Sept

More information

thebiotutor.com AS Biology Unit 2 Classification, Adaptation & Biodiversity

thebiotutor.com AS Biology Unit 2 Classification, Adaptation & Biodiversity thebiotutor.com AS Biology Unit 2 Classification, Adaptation & Biodiversity 1 Classification and taxonomy Classification Phylogeny Taxonomy The process of sorting living things into groups. The study of

More information

OMICS Journals are welcoming Submissions

OMICS Journals are welcoming Submissions OMICS Journals are welcoming Submissions OMICS International welcomes submissions that are original and technically so as to serve both the developing world and developed countries in the best possible

More information

DNA barcoding is a technique for characterizing species of organisms using a short DNA sequence from a standard and agreed-upon position in the

DNA barcoding is a technique for characterizing species of organisms using a short DNA sequence from a standard and agreed-upon position in the DNA barcoding is a technique for characterizing species of organisms using a short DNA sequence from a standard and agreed-upon position in the genome. DNA barcode sequences are very short relative to

More information

Post-doc fellowships to non-eu researchers FINAL REPORT. Home Institute: Centro de Investigaciones Marinas, Universidad de La Habana, CUBA

Post-doc fellowships to non-eu researchers FINAL REPORT. Home Institute: Centro de Investigaciones Marinas, Universidad de La Habana, CUBA Recipient: Maickel Armenteros Almanza. Post-doc fellowships to non-eu researchers FINAL REPORT Home Institute: Centro de Investigaciones Marinas, Universidad de La Habana, CUBA Promoter: Prof. Dr. Wilfrida

More information

CREATING PHYLOGENETIC TREES FROM DNA SEQUENCES

CREATING PHYLOGENETIC TREES FROM DNA SEQUENCES INTRODUCTION CREATING PHYLOGENETIC TREES FROM DNA SEQUENCES This worksheet complements the Click and Learn developed in conjunction with the 2011 Holiday Lectures on Science, Bones, Stones, and Genes:

More information

Phylogeny and systematics. Why are these disciplines important in evolutionary biology and how are they related to each other?

Phylogeny and systematics. Why are these disciplines important in evolutionary biology and how are they related to each other? Phylogeny and systematics Why are these disciplines important in evolutionary biology and how are they related to each other? Phylogeny and systematics Phylogeny: the evolutionary history of a species

More information

DNA Sequencing as a Method for Larval Identification in Odonates

DNA Sequencing as a Method for Larval Identification in Odonates DNA Sequencing as a Method for Larval Identification in Odonates Adeline Harris 121 North St Apt 3 Farmington, ME 04938 Adeline.harris@maine.edu Christopher Stevens 147 Main St Apt 1 Farmington, ME 04938

More information

Big Idea 1: Does the process of evolution drive the diversity and unit of life?

Big Idea 1: Does the process of evolution drive the diversity and unit of life? AP Biology Syllabus 2016-2017 Course Overview: AP Biology is equivalent to an introductory college level biology program in order to develop student led inquiry into science. The class is designed to go

More information

Jisha Krishnan and Sebastian, IJALS, Volume (8) Issue (2) May RESEARCH ARTICLE

Jisha Krishnan and Sebastian, IJALS, Volume (8) Issue (2) May RESEARCH ARTICLE Analysis of evolutionary divergence of Neurothemis tullia (Odonata:Libellulidae) using cytochrome oxidase subunit I gene E.K. Jisha Krishnan and C.D. Sebastian Molecular Biology Laboratory, Department

More information

THE DISTRIBUTION OF THE PARASITIC GASTROPOD CALEDONIELLA MONTROUZIERI SOUVERBIE, 1869 (CALEDONIELLIDAE), ON GONODACTYLID STOMATOPOD CRUSTACEANS

THE DISTRIBUTION OF THE PARASITIC GASTROPOD CALEDONIELLA MONTROUZIERI SOUVERBIE, 1869 (CALEDONIELLIDAE), ON GONODACTYLID STOMATOPOD CRUSTACEANS THE DISTRIBUTION OF THE PARASITIC GASTROPOD CALEDONIELLA MONTROUZIERI SOUVERBIE, 1869 (CALEDONIELLIDAE), ON GONODACTYLID STOMATOPOD CRUSTACEANS ABSTRACT. - The gastropod mollusc Caledoniella montrouzieri

More information

Bacteria, Friends or Foes?

Bacteria, Friends or Foes? Bacteria, Friends or Foes? This unit integrates molecular biology techniques with the role of bacteria in our environment, specifically in the marine environment. The unit starts with introductory activities

More information

Phylogenetic Analysis of Reticulitermes speratus using the Mitochondrial Cytochrome C Oxidase Subunit I Gene* 1

Phylogenetic Analysis of Reticulitermes speratus using the Mitochondrial Cytochrome C Oxidase Subunit I Gene* 1 Mokchae Konghak 38(2) : 135~139, 2010 Phylogenetic Analysis of Reticulitermes speratus using the Mitochondrial Cytochrome C Oxidase Subunit I Gene* 1 Moon Jung Cho* 2, Keum Shin* 2, Young-Kyoon Kim* 2,

More information

Draft document version 0.6; ClustalX version 2.1(PC), (Mac); NJplot version 2.3; 3/26/2012

Draft document version 0.6; ClustalX version 2.1(PC), (Mac); NJplot version 2.3; 3/26/2012 Comparing DNA Sequences to Determine Evolutionary Relationships of Molluscs This activity serves as a supplement to the online activity Biodiversity and Evolutionary Trees: An Activity on Biological Classification

More information

Deep sequencing to understand the marine environment. Jan Pawlowski Department of Genetics and Evolution University of Geneva

Deep sequencing to understand the marine environment. Jan Pawlowski Department of Genetics and Evolution University of Geneva Deep sequencing to understand the marine environment Jan Pawlowski Department of Genetics and Evolution University of Geneva Known 1/10 gap In the Ocean the number of predicted species is about 10 times

More information

CHAPTERS 24-25: Evidence for Evolution and Phylogeny

CHAPTERS 24-25: Evidence for Evolution and Phylogeny CHAPTERS 24-25: Evidence for Evolution and Phylogeny 1. For each of the following, indicate how it is used as evidence of evolution by natural selection or shown as an evolutionary trend: a. Paleontology

More information

Bayesian species identification under the multispecies coalescent provides significant improvements to DNA barcoding analyses

Bayesian species identification under the multispecies coalescent provides significant improvements to DNA barcoding analyses Molecular Ecology (2017 26, 3028 3036 doi: 10.1111/mec.14093 Bayesian species identification under the multispecies coalescent provides significant improvements to DNA barcoding analyses ZIHENG YANG* and

More information

Microbial DNA qpcr Multi-Assay Kit Clostridium perfringens Pathogenicity

Microbial DNA qpcr Multi-Assay Kit Clostridium perfringens Pathogenicity Microbial DNA qpcr Multi-Assay Kit Clostridium perfringens Pathogenicity Cat. no. 330043 BBID-1507ZR-3 For real-time PCR-based, application-specific microbial identification or profiling The Clostridium

More information

Identification of COI partial sequences in two closely related frog species, Rana dalmatina and Rana temporaria

Identification of COI partial sequences in two closely related frog species, Rana dalmatina and Rana temporaria HERPETOLOGICA ROMANICA Vol. 4, 2010, pp.1-6 ISSN: 1842-9203 Article No. 041101 Identification of COI partial sequences in two closely related frog species, Rana dalmatina and Rana temporaria Béla MAROSI

More information

Warm-Up- Review Natural Selection and Reproduction for quiz today!!!! Notes on Evidence of Evolution Work on Vocabulary and Lab

Warm-Up- Review Natural Selection and Reproduction for quiz today!!!! Notes on Evidence of Evolution Work on Vocabulary and Lab Date: Agenda Warm-Up- Review Natural Selection and Reproduction for quiz today!!!! Notes on Evidence of Evolution Work on Vocabulary and Lab Ask questions based on 5.1 and 5.2 Quiz on 5.1 and 5.2 How

More information

Recent Harmful Algal blooms (HABs) Events in Indonesia

Recent Harmful Algal blooms (HABs) Events in Indonesia Recent Harmful Algal blooms (HABs) Events in Indonesia By Hikmah Thoha Research Center for Oceanography, Indonesian Institute of Science Indonesia What We Know, and What We Do Not Know on HABs Outline

More information

Outline. Classification of Living Things

Outline. Classification of Living Things Outline Classification of Living Things Chapter 20 Mader: Biology 8th Ed. Taxonomy Binomial System Species Identification Classification Categories Phylogenetic Trees Tracing Phylogeny Cladistic Systematics

More information

PHYLOGENY AND SYSTEMATICS

PHYLOGENY AND SYSTEMATICS AP BIOLOGY EVOLUTION/HEREDITY UNIT Unit 1 Part 11 Chapter 26 Activity #15 NAME DATE PERIOD PHYLOGENY AND SYSTEMATICS PHYLOGENY Evolutionary history of species or group of related species SYSTEMATICS Study

More information

Macroevolution Part I: Phylogenies

Macroevolution Part I: Phylogenies Macroevolution Part I: Phylogenies Taxonomy Classification originated with Carolus Linnaeus in the 18 th century. Based on structural (outward and inward) similarities Hierarchal scheme, the largest most

More information

PLEASE SCROLL DOWN FOR ARTICLE. Full terms and conditions of use:

PLEASE SCROLL DOWN FOR ARTICLE. Full terms and conditions of use: This article was downloaded by: [University of Queensland] On: 2 February 2010 Access details: Access Details: [subscription number 731609550] Publisher Taylor & Francis Informa Ltd Registered in England

More information

Chapter 26 Phylogeny and the Tree of Life

Chapter 26 Phylogeny and the Tree of Life Chapter 26 Phylogeny and the Tree of Life Biologists estimate that there are about 5 to 100 million species of organisms living on Earth today. Evidence from morphological, biochemical, and gene sequence

More information

A Study of the Moss Parasite Eocronartium muscicola By: Alicia Knudson Advisor: Dr. Elizabeth Frieders

A Study of the Moss Parasite Eocronartium muscicola By: Alicia Knudson Advisor: Dr. Elizabeth Frieders A Study of the Moss Parasite Eocronartium muscicola By: Alicia Knudson Advisor: Dr. Elizabeth Frieders Abstract The genus Eocronartium contains a single described species of parasitic fungus on moss plants

More information

Prereq: Concurrent 3 CH

Prereq: Concurrent 3 CH 0201107 0201101 General Biology (1) General Biology (1) is an introductory course which covers the basics of cell biology in a traditional order, from the structure and function of molecules to the structure

More information

ANALYSIS OF PHYLOGENETIC RELATIONSHIP AMONG CARANGOIDES SPECIES USING MEGA 6

ANALYSIS OF PHYLOGENETIC RELATIONSHIP AMONG CARANGOIDES SPECIES USING MEGA 6 ANALYSIS OF PHYLOGENETIC RELATIONSHIP AMONG CARANGOIDES SPECIES USING MEGA 6 NIVETHA SARAH EBENEZER 1, JOYCE PRIYAKUMARI C 2* 1Research Assistant, Bioinformatics Infrastructure Facility (BIF), BTIS Net

More information

Bioinformatics. Dept. of Computational Biology & Bioinformatics

Bioinformatics. Dept. of Computational Biology & Bioinformatics Bioinformatics Dept. of Computational Biology & Bioinformatics 3 Bioinformatics - play with sequences & structures Dept. of Computational Biology & Bioinformatics 4 ORGANIZATION OF LIFE ROLE OF BIOINFORMATICS

More information

PHYLOGENY WHAT IS EVOLUTION? 1/22/2018. Change must occur in a population via allele

PHYLOGENY WHAT IS EVOLUTION? 1/22/2018. Change must occur in a population via allele PHYLOGENY EXERCISE 1 AND 2 WHAT IS EVOLUTION? The theory that all living organisms on earth are related and have a common ancestor. These organism have changed over time and are continuing to change. Changes

More information

DNA Barcoding Analysis of matk Gene of Some Syzygium Species

DNA Barcoding Analysis of matk Gene of Some Syzygium Species DNA Barcoding Analysis of matk Gene of Some Syzygium Species Trina E. Tallei*, Presticilla D. Irawan, Beivy J. Kolondam Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi

More information

Missouri Educator Gateway Assessments

Missouri Educator Gateway Assessments Missouri Educator Gateway Assessments June 2014 Content Domain Range of Competencies Approximate Percentage of Test Score I. Science and Engineering Practices 0001 0003 21% II. Biochemistry and Cell Biology

More information

Building the Tree of Life

Building the Tree of Life 18.3 Building the Tree of Life Changing Ideas About Kingdoms This diagram shows some of the ways in which organisms have been classified into kingdoms since the 1700s. Three Domains Genetic analysis has

More information

RECOMMENDED STUDY PLAN 2018

RECOMMENDED STUDY PLAN 2018 RECOMMENDED STUDY PLAN 2018 NAME DEGREE PROGRAM Master of Science STUDENT NUMBER MAJOR Trop Bio & Con-TSV (MSC-TBI) Level 5: Year 1 SP3 SP1 SP6/7 SP2 SP10/SP11 Major Core: BZ5215 Major Opt Skill Conservation

More information

Conservation Genetics. Outline

Conservation Genetics. Outline Conservation Genetics The basis for an evolutionary conservation Outline Introduction to conservation genetics Genetic diversity and measurement Genetic consequences of small population size and extinction.

More information

DNA Barcoding: A New Tool for Identifying Biological Specimens and Managing Species Diversity

DNA Barcoding: A New Tool for Identifying Biological Specimens and Managing Species Diversity DNA Barcoding: A New Tool for Identifying Biological Specimens and Managing Species Diversity DNA barcoding has inspired a global initiative dedicated to: Creating a library of new knowledge about species

More information

Microbial Diversity. Yuzhen Ye I609 Bioinformatics Seminar I (Spring 2010) School of Informatics and Computing Indiana University

Microbial Diversity. Yuzhen Ye I609 Bioinformatics Seminar I (Spring 2010) School of Informatics and Computing Indiana University Microbial Diversity Yuzhen Ye (yye@indiana.edu) I609 Bioinformatics Seminar I (Spring 2010) School of Informatics and Computing Indiana University Contents Microbial diversity Morphological, structural,

More information

PHYLOGENY & THE TREE OF LIFE

PHYLOGENY & THE TREE OF LIFE PHYLOGENY & THE TREE OF LIFE PREFACE In this powerpoint we learn how biologists distinguish and categorize the millions of species on earth. Early we looked at the process of evolution here we look at

More information

A. Correct! Taxonomy is the science of classification. B. Incorrect! Taxonomy is the science of classification.

A. Correct! Taxonomy is the science of classification. B. Incorrect! Taxonomy is the science of classification. DAT - Problem Drill 07: Diversity of Life Question No. 1 of 10 Instructions: (1) Read the problem and answer choices carefully, (2) Work the problems on paper as 1. What is taxonomy? Question #01 (A) Taxonomy

More information

8/23/2014. Introduction to Animal Diversity

8/23/2014. Introduction to Animal Diversity Introduction to Animal Diversity Chapter 32 Objectives List the characteristics that combine to define animals Summarize key events of the Paleozoic, Mesozoic, and Cenozoic eras Distinguish between the

More information

ABSTRACT: 390 GENETIC VARIABILITY OF MTDNA FROM DIABROTICA SPECIOSA (GERMAR, 1824) (COLEOPTERA: CHYSOMELIDAE) BRAZILIAN POPULATIONS

ABSTRACT: 390 GENETIC VARIABILITY OF MTDNA FROM DIABROTICA SPECIOSA (GERMAR, 1824) (COLEOPTERA: CHYSOMELIDAE) BRAZILIAN POPULATIONS ABSTRACT: 390 GENETIC VARIABILITY OF MTDNA FROM DIABROTICA SPECIOSA (GERMAR, 1824) (COLEOPTERA: CHYSOMELIDAE) BRAZILIAN POPULATIONS YANO S A C¹, SILVA-BRANDÃO K L², AFONSO A P S³, MARTINELLI S 4 & SOSA-GÓMEZ

More information

Analysis of putative DNA barcodes for identification and distinction of native and invasive plant species

Analysis of putative DNA barcodes for identification and distinction of native and invasive plant species Babson College Digital Knowledge at Babson Babson Faculty Research Fund Working Papers Babson Faculty Research Fund 2010 Analysis of putative DNA barcodes for identification and distinction of native and

More information

Gear data report from Atlantic plankton cruises for the R/V Pathfinder, March March 1962

Gear data report from Atlantic plankton cruises for the R/V Pathfinder, March March 1962 College of William and Mary W&M ScholarWorks Reports 1962 Gear data report from Atlantic plankton cruises for the R/V Pathfinder, March 1961 - March 1962 Woodrow L. Wilson Virginia Institute of Marine

More information

fglobal phylogeography and species delimitation in the sexy shrimp Thor amboinensis Undergraduate Research Thesis

fglobal phylogeography and species delimitation in the sexy shrimp Thor amboinensis Undergraduate Research Thesis fglobal phylogeography and species delimitation in the sexy shrimp Thor amboinensis Undergraduate Research Thesis Presented in Partial Fulfillment of the Requirements for graduation with Honors Research

More information

SPECIES PARADOX By Colin leslie dean

SPECIES PARADOX By Colin leslie dean SPECIES PARADOX By Colin leslie dean B,Sc, BA, B.Litt(Hons), MA, B.Litt(Hons), MA, MA (Psychoanalytic studies), Master of Psychoanalytic studies, Grad Cert (Literary studies) Gamahucher press West Geelong

More information

Appraisal of the entire mitochondrial genome for DNA barcoding in birds

Appraisal of the entire mitochondrial genome for DNA barcoding in birds Progress in Biological Sciences Vol. 4, Number 2, Summer / Autumn 2014/167-178 Appraisal of the entire mitochondrial genome for DNA barcoding in birds Received: January 5, 2014; Accepted: March 30, 2014

More information

MiGA: The Microbial Genome Atlas

MiGA: The Microbial Genome Atlas December 12 th 2017 MiGA: The Microbial Genome Atlas Jim Cole Center for Microbial Ecology Dept. of Plant, Soil & Microbial Sciences Michigan State University East Lansing, Michigan U.S.A. Where I m From

More information

Diversity of Organisms and Classification

Diversity of Organisms and Classification Diversity of Organisms and Classification Essential Vocabulary DNA Prokaryotic: : a cell that lacks a nucleus. Eukaryotic: : a cell that has a nucleus and other complex structures Feeding Autotrophic:

More information

Using Ensembles of Hidden Markov Models for Grand Challenges in Bioinformatics

Using Ensembles of Hidden Markov Models for Grand Challenges in Bioinformatics Using Ensembles of Hidden Markov Models for Grand Challenges in Bioinformatics Tandy Warnow Founder Professor of Engineering The University of Illinois at Urbana-Champaign http://tandy.cs.illinois.edu

More information

History and meaning of the word Ecology A. Definition 1. Oikos, ology - the study of the house - the place we live

History and meaning of the word Ecology A. Definition 1. Oikos, ology - the study of the house - the place we live History and meaning of the word Ecology A. Definition 1. Oikos, ology - the study of the house - the place we live B. Etymology study of the origin and development of a word 1. Earliest - Haeckel (1869)

More information

CHEM 1101 and CHEM 1102 and CHEM 1103 or CHEM 1211 and CHEM 1212 and CHEM CHEM 1214 and CHEM 1215 and CHEM 1216

CHEM 1101 and CHEM 1102 and CHEM 1103 or CHEM 1211 and CHEM 1212 and CHEM CHEM 1214 and CHEM 1215 and CHEM 1216 Environmental Science, BS 1 Environmental Science, BS Our Bachelor of Science in Environmental Science degree is organized for students who seek a comprehensive understing of the scientific implications

More information

1 of 13 8/11/2014 10:32 AM Units: Teacher: APBiology, CORE Course: APBiology Year: 2012-13 Chemistry of Life Chapters 1-4 Big Idea 1, 2 & 4 Change in the genetic population over time is feedback mechanisms

More information

USING MORPHOLOGICAL AND MOLECULAR TECHNIQUES FOR THE IDENTIFICATION OF WHITE GRUB SPECIES (COLEOPTERA: SCARABAEIDAE)

USING MORPHOLOGICAL AND MOLECULAR TECHNIQUES FOR THE IDENTIFICATION OF WHITE GRUB SPECIES (COLEOPTERA: SCARABAEIDAE) SHORT, NON-REFEREED PAPER USING MORPHOLOGICAL AND MOLECULAR TECHNIQUES FOR THE IDENTIFICATION OF WHITE GRUB SPECIES (COLEOPTERA: SCARABAEIDAE) MGOCHEKI N 1, CONLONG DE 1,2, GANESHAN S 3 AND ADDISON P 1

More information

BIOINFORMATICS: An Introduction

BIOINFORMATICS: An Introduction BIOINFORMATICS: An Introduction What is Bioinformatics? The term was first coined in 1988 by Dr. Hwa Lim The original definition was : a collective term for data compilation, organisation, analysis and

More information

Model Based Spatial For Monitoring Surveillance Of Fisheries Ships To Fishing Area At Aru Sea, Arafura Sea And East Timor Sea In FMARI 718

Model Based Spatial For Monitoring Surveillance Of Fisheries Ships To Fishing Area At Aru Sea, Arafura Sea And East Timor Sea In FMARI 718 The International Journal of Engineering and Science (IJES) Volume 7 Issue 10 Ver.I Pages PP 01-07 2018 ISSN (e): 2319 1813 ISSN (p): 23-19 1805 Model Based Spatial For Monitoring Surveillance Of Fisheries

More information

Taxonomy and Biodiversity

Taxonomy and Biodiversity Chapter 25/26 Taxonomy and Biodiversity Evolutionary biology The major goal of evolutionary biology is to reconstruct the history of life on earth Process: a- natural selection b- mechanisms that change

More information

Oceanography Page 1 of 9 Lab: Pond Water M.Sewell rm #70

Oceanography Page 1 of 9 Lab: Pond Water M.Sewell rm #70 Oceanography Page 1 of 9 Pond Water Lab Introduction: Why Study Pond Microlife? Right, what are all those little things really good for? Well, for one thing, without bacteria no fish, no frogs, no birds,

More information

Supporting Information

Supporting Information Supporting Information Ziemert et al. 10.1073/pnas.1324161111 Fig. S1. Geographic origin and numbers of Salinispora strains used in this study. Fig. S2. Operational biosynthetic unit (OBU) phylogeny supports

More information

Biology: Life on Earth

Biology: Life on Earth Biology: Life on Earth Eighth Edition Lecture for Chapter 1 An Introduction to Life on Earth Section 1.3 Outline 1.3 What Are the Characteristics of Living Things? Living Things Are Both Complex, Organized,

More information

Biology 211 (2) Week 1 KEY!

Biology 211 (2) Week 1 KEY! Biology 211 (2) Week 1 KEY Chapter 1 KEY FIGURES: 1.2, 1.3, 1.4, 1.5, 1.6, 1.7 VOCABULARY: Adaptation: a trait that increases the fitness Cells: a developed, system bound with a thin outer layer made of

More information

Creating an e-flora for South Africa

Creating an e-flora for South Africa SANBI POLICY DOCUMENT DIVISION: Biosystematics Research and Biodiversity Collections EFFECTIVE DATE: 1 April 2014 Compiler: Marianne le Roux & Janine Victor POLICY NUMBER: LAST AMENDED: Creating an e-flora

More information