colony size color morphology haemolysis S. aureus S. epidermidis
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1 practical 2.: STAPHYLOCOCCUS 1. Prepare a heat fixed smear of the culture of S.aureus. (Gram staining, microscopy). 2. Prepare a heat fixed smear of the culture of S.aureus. and S.epidermidis (mixed smear), (Gram staining, microscopy). 3. Stain fixed smear prepared from the clinical sample (pus, Gram staining, microscopy). 4. In vitro cultivation of staphylococci - S.aureus a S.epidermidis (blood agar) colony size color morphology haemolysis S. aureus S. epidermidis
2 5. Biochemical properties of S.aureus a S.epidermidis (MANNITOL SALT agar). 6. Catalase reaction Slide coagulase test Tube coagulase test Biochemical identification of staphylococci STAPHYTEST 16 (principle, demonstration) Staphylococci - ATB susceptibility
3 Practical 3. : STREPTOCOCCI 1. Prepare a heat fixed smear of the broth culture of S.pyogenes (Gram staining, microscopy). 1. Prepare a heat fixed smear of the culture of S. pneumoniae (Gram staining, microscopy). 2. Streptococcus in vitro cultivation (blood agar). S.pyogenes S. agalactiae S. salivarius S. pneumoniae
4 colony size color morphology haemolysis S. pyogenes S. agalactiae S.salivarius S. pneumoniae 3. Bacitracin test (S. pyogenes, S. agalactiae). S.pyogenes S. agalactiae CAMP test (S. pyogenes, S. agalactiae). S.pyogenes S. agalactiae
5 Optochin test (S. salivarius, S. pneumoniae). S. salivarius S. pneumoniae Bile solubility test (S. pneumoniae )
6 7. Antistreptolysin O (principle, results interpretation) C-reactive protein ( CRP) Streptococci ATB susceptibility
7 Practical 4.: NEISSERIA, HAEMOPHILLUS 1. Stain fixed smear prepared from the clinical sample (pus, Gram staining). (N. gonorrhoeae) Prepare a heat fixed smear of the culture of H. influenzae (Gram staining) Microscopy fixed smear - clinical sample (cerebrospinal fluid, N. meningitidis).....
8 4. Cultivation - N. gonorrhoeae - Chocolate agar (CHOC) Cultivation- H. influenzae blood agar, Chocolate agar (CHOC). BA CHOC Cultivation swab of tonsils, blood agar.....
9 7. Catalase test - N. gonorrhoeae Oxidase test Growth factors - H. influenzae, H. parainfluenzae ATB susceptibility - H. influenzae and N. gonorrhoeae
10 Practical 5.: ENTEROBAKTERIACEAE 1. Prepare a heat fixed smear of the culture of E. coli (Gram staining) Prepare a heat fixed smear of the culture of Salmonella sp. (Gram staining). 3. Prepare a heat fixed smear of the culture of K. pneumoniae (Burri ink staining).
11 4. Cultivation - E. coli, K. pneumoniae, Salmonella sp., Shigella. sp. dan P. vulgaris blood agar, Endo agar (EA), Deoxycholatcitrate agar (DCA). Blood agar E.coli K. pneumoniae Shigella sp. Salmonella sp. P. vulgaris P. mirabilis colony Endo agar E.coli K. Shigella sp. Salmonella P. vulgaris P. pneumoniae sp. mirabilis Lactose ferm. color DC agar E.coli K. Shigella sp. Salmonella P. vulgaris P. pneumoniae sp. mirabilis Lactose ferm. H 2 S
12 5. Rauss phenomenon. 6. Biochemical properties - E. coli, K. pneumoniae, Salmonella sp., Shigella. sp., P. mirabilis, and P. vulgaris ( Triple Sugar Iron Agar - TSI). E.coli K. Shigella sp. Salmonella P. vulgaris P. pneumoniae sp. mirabilis dextrose, lactose, and sucrose fermentation gas hydrogen sulfide production
13 Enterobacteriaceae Biochemical activity testing (enterotest)
14 8. Serotypisation of E. coli (polyvalent and monovalent sera, principle) Widal reaction ATB susceptibility testing E. coli, K. pneumoniae, Salmonella sp., Shigella. sp., P. mirabilis, and P. vulgaris
15 Practical 6.: A. Grampositive rods. B. Pseudomonas aeruginosa 1. Prepare a heat fixed smear of the culture of Pseudomonas aeruginosa (Gram staining) Stain fixed smear of the culture of B. anthracis (Gram staining) Prepare a heat fixed smear of the culture of B.cereus (Gram staining)......
16 4. Stain fixed smear of the culture of B. anthracis (Wirtz-Conklin staining procedure) Cultivation - Pseudomonas aeruginosa (blood agar, DC agar). BA DC 6. Cultivation - B. anthracis, B.cereus (blood agar). B. anthracis B.cereus
17 7. Biochemical activity testing - Pseudomonas aeruginosa and nonfermentative G-negative rods (TSI agar, oxidase test) ATB susceptibility - Pseudomonas aeruginosa, B. anthracis
18 Practical 7.: CORYNEBACTERIUM, BORDETELLA 1. Stain fixed smear of the culture of C. diphteriae (Gram staining) Prepare a heat fixed smear of the culture of C. pseudodiphteriticum (Gram staining) Stain fixed smear of the culture of C. diphteriae (Albert staining)......
19 4. Stain fixed smear of the culture of B. pertussis (Gram staining) Cultivation - C. diphteriae and C. pseudodiphteriticum (blood agar, Tellurite agar) Cultivation - B. pertussis (Bordet - Gengou agar)......
20 7. Toxigenicity of C. diphteriae (in vitro- immunodiffusion, in vivo) ATB susceptibility - B. pertussis, C. diphteriae a C. pseudodiphteriae
21 Practical 8.: ANAEROBIC BACTERIA, SPIROCHETES. 1. Cultivation media for anaerobic bacteria Anaerobes - specimen colection and transport of biologic material Anaerobic culture methods: a. Biological (Serratia marcescens): b. Physical:
22 c. Chemical: Anaerobic bacteria : microscopy (Gram staining). Propionibacterium Clostridium difficile Clostridium perfringens Peptostreptococcus sp. acnes 5. Laboratory diagnosis of syphilis (nontreponemal and treponemal tests)
23 Practical 9.: MYCOBACTERIUM 1. Stain fixed smear of the culture of M. tuberculosis (Ziehl-Neelsen staining) M. tuberculosis in vitro cultivation (liquid an solid cultivation media) M. tuberculosis (fluorescent microscopy)......
24 Practical 10.: VIROLOGY 1. Laboratory diagnosis of viral diseases direct methods (electron microscopy, virus isolation) Laboratory diagnosis of viral diseases indirect methods (serologic diagnostic methods)
25 Practical 11.: PARASITOLOGY 1. Specimen colection, transport of biological materials Enterobius vermicularis (Pinworm) microscopy (eggs) Microscopy (biologic material stool)......
26
27 Practical 12.: FUNGI 1. Prepare a heat fixed smear of the culture of C. albicans (Gram staining) Culture yeasts and molds (Candida sp., Aspergillus, Sabouraud agar). Candida Aspergillus Culture and microscopy - C. albicans on rice agar......
28
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