BIOL 3702L: MICROBIOLOGY LABORATORY SCHEDULE, SUMMER 2015

Transcription

1 BIOL 3702L: MICROBIOLOGY LABORATORY SCHEDULE, SUMMER 2015 Week of May 18 th Introduction to the Microbiology Laboratory: Become familiar with the laboratory and its safety features Review safety rules Exercise 1: [INDIVIDUAL] Pages 4-6, Procedure for Basic Microscopy (Perform All Steps) Lab Report: Answer Questions 1-14, pages Exercise 7: [INDIVIDUAL REVIEW ONLY!!!] Pages 42-43, Smear Preparation and Simple Staining NOTE: Review this lab exercise to learn the basics of making a smear and how fix and stain such preparations. Exercise 8: [INDIVIDUAL] Pages 49-50, Procedure for Traditional Gram-Stain Technique (All Steps); and Control Procedure (All Steps) Lab Report Answer All Questions, pages Laboratory Safety Rules Quiz Materials: Prepared slides of cocci, rods, and spirilla Newspaper Scissors TSB culture of Escherichia coli (8) TSB culture of Staphylococcus aureus (8) TSB culture of Escherichia coli and Staphylococcus aureus mixture (8) Hot plate/slide warmer Page 1 of 6

2 Week of May 25 th University Holiday: Laboratory Closed Exercise 14: [INDIVIDUAL] Pages 91-92, Aseptic Technique and Procedure for Isolation of Pure Cultures and Their Maintenance (Perform All Steps for Aseptic Technique ONLY) Lab Report: Answer All Questions, pages Exercise 16: [INDIVIDUAL] Pages , Principles of the Streak Plate Technique (Perform Steps 5-7); and Principles for the Use of Selective and Differential Media (Perform Steps 5-6) Lab Report: Answer Questions 1 and 3 (page 108) and All Questions on page 109 NOTE: Students will need to return to the laboratory on Thursday or Friday to examine the results of their work. Skills Test I Microscopy Instructions available at the following URL: TSB culture of Serratia marcescens (14) TSA culture of Serratia marcescens (14) TSA streak plate of Serratia marcescens (14) TSA streak plate of Bacillus subtilis (14) TSA streak plate of Escherichia coli (14) TSB culture of Escherichia coli and Serratia marcescens mixture (16) TSB culture of Escherichia coli and Staphylococcus aureus mixture (16) TSB tubes (14) TSA slants (14) TSA deeps (14) TSA plates (16) EMB agar plate (16) MSA plate (16) Prepared slides of cocci, rods, and spirilla (Skills Test I) Page 2 of 6

3 Week of June 1 st Exercise 19: [GROUP WORK] Pages , Standard Plate Count (All Steps) Lab Report Answer Question 1, page 130 Hand Washing Exercise [GROUP WORK] Download the directions at the following URL: Skills Test II Streak Plate (begin) Skills Test III Gram Stain Instructions for both Skills Tests are available at the following URL: TSB culture of Escherichia coli (19) TSB culture of Escherichia coli and Serratia marcescens mixture (Skills Test II) TSB culture of Escherichia coli and Staphylococcus aureus mixture (Skills Test III) Sterile petri dishes (19) Saline (or phosphate buffer) blank bottles 99 ml (19) Plate count agar (molten, held at C) (19) TSA plates Hand soaps (three various types) in pump bottles Hot plate/slide warmer Complete Exercise 19 and Hand Washing Exercise Skills Test II Streak Plate (submit plates for scoring; repeat Skills Test if permitted by laboratory instructor) Skills Test III due TSB culture of Escherichia coli and Serratia marcescens mixture (Skills Test II) TSA plate (Skills Test II) Page 3 of 6

4 Week of June 8 th [ALL OF THESE EXERCISES ARE GROUP WORK] Exercise 20: Page 138, Durham Tube, First and Second Period (All Steps) Lab Report Answer All Questions, pages Exercise 21: Page 144, First and Second Period (All Steps) Lab Report Answer All Questions, pages Exercise 24: Page , First and Second Period (All Steps) Lab Report Answer All Questions, pages Exercise 25: Page , All Four Tests, First and Second Period (All Steps) Lab Report Answer All Questions, pages Exercise 27: Page , First and Second Period (All Steps EXCEPT Key Rapid Test) Lab Report Answer All Questions/Enter Data on page 179 (EXCEPT KEY Strips and Questions 1-6 on page 180 Exercise 28: Page 182, First and Second Period (All Steps) Lab Report Answer All Questions, pages ASSIGNMENTS CONTINUED ON NEXT PAGE TSB cultures of Escherichia coli (20, 21, 25, 27), Alkaligenes faecalis (20, 21), Salmonella cholera-suis [or suitable substitute] (20, 24), Proteus vulgaris (21, 24, 25, 27), Pseudomonas aeruginosa (21), Enterococcus faecalis (28), Enterobacter aeorgenes (25, 27), Micrococcus luteus (28), Klebsiella pneumoniae (24, 25), Staphylococcus aureus (28), and Shigella flexneri [or suitable substitute] (21) Sabouraud dextrose agar plate culture of Saccharomyces cerevisiae (20) Carbohydrate fermentation broths [dextrose, lactose, and sucrose] with Durham tube (20) Triple sugar iron (TSI) slants (21) Sulfide-indole-motility (SIM) deeps (24, 25) Motility test medium (with tetrazolium) deeps (24) Kovacs reagent (25) MR-VP broth tubes (25) Test tubes (25) Methyl red indicator (25) Barritt s A and B solutions (25) Simmons citrate agar slants (25) Nutrient gelatin deeps (27) TSA slants (28) 3% hydrogen peroxide (28) Page 4 of 6

5 Week of June 8 th (cont.) Exercise 29: Pages , First and Second Period (DNase Test only) Lab Report Answer Questions 1 and 2 on page 189 and Questions 2, 5, 6, and 7 on page 190 Exercise 30: Pages , First Period (All Steps) and Second Period (Oxidase Reagent only) Lab Report Answer Questions 1-2 (with relevant data only), page 147 and Questions 1-6, page 196 Exercise 31: Page 199, First and Second Period (Except Disk or Tablet Steps) Lab Report Answer Questions 1-2 (with relevant data only), page 200 and All Questions, page 201 Exercise 32: Pages , First and Second Period (Except Tablet or Strip Steps) Lab Report Answer Questions 1-3 (with relevant data only), page 206 and All Questions, page 207 Exercise 33: Pages , First Period (Steps 1-3) and Second Period (All Steps) Lab Report Answer Questions 1-3 (with relevant data only), page 211 and All Questions, page 212 Exercise 34: Pages 216, First and Second Period (All Steps) Lab Report Answer All Questions, pages TSB cultures of Escherichia coli (30, 31, 33, 34), Alkaligenes faecalis (30), Pseudomonas aeruginosa (30, 34), Klebsiella pneumoniae (31, 32), Proteus vulgaris (31, 32, 33), Salmonella cholera-suis [or suitable substitute] (31), Enterobacter aeorgenes (32), Citrobacter fruendii (32), and Staphylococcus epidermidis (34) TSA slant cultures of Staphylococcus aureus (29) and Staphylococcus epidermidis (29) DNase plates (29) TSA plates (30) Oxidase reagent (fresh) (30) Urea broth tubes or agar slants (21) Lysine decarboxylase broth tubes (32) Ornithine decarboxylase broth tubes (32) Arginine decarboxylase broth tubes (32) Decarboxylase control broth tubes (32) Lysine iron agar (LIA) slants (32) Mineral oil (32) Phenylalanine deaminase agar slants (33) 10% ferric chloride solution (33) Nitrate broths with Durham tubes (34) Soil (34) Nitrate test reagents A and B (34) Zinc powder (34) Page 5 of 6

6 Week of June 8 th (cont.) Complete All Diagnostic Tests Materials: All materials necessary to complete the assigned exercises from Monday s laboratory Week of June 15 th Skill Test IV Bacterial Unknown Instructions for both Skills Tests are available at the following URL: Materials: Numbered TSB cultures of unknown bacterial mixtures TSA slants (for student stock cultures) Continue with Skills Test IV Materials: Week of June 22 nd Continue with Skills Test IV Materials: Complete Skills Test IV Skills Test IV Report Sheets Due By End of the Laboratory Period Materials: Page 6 of 6