Development of Compact Dry SL for Salmonella detection
|
|
- Winfred Hodge
- 6 years ago
- Views:
Transcription
1 Development of Compact Dry SL for Salmonella detection S.Mizuochi 1),H.Teramura 1),S.Nirazuka 1),H.Kodaka 1),K.Inoue ) K.Tamura 3) 1)Research Dept., Nissui Pharmaceutical, ) Saitama Pref. Ranzango Hp., 3)National Institute of Infectious Diseases
2 Background Normally, Salmonella detection from food sample is processed these following steps, Pre-culture, Selective enrichment culture, Isolation and Confirmation/Identification. There are strong customer demands to improve the test procedure for more simple and rapid one, if it could have an equivalent result. We develop simple and easy medium for Salmonella detection (Compact Dry SL, hereinafter CDSL) that detects Salmonella by combination of biochemical reaction, specific enzyme reaction and detection of motility of Salmonella. CDSL is screening method for detecting Salmonella which inoculate pre-culture sample as a specimen. Herewith we report CDSL evaluation results compared with conventional culture methods using meats on the market, 1 growth and color forming used by pure culture strains recovery of Salmonella from food materials 3 comparison (sensitivity/specificity/agreement) between conventional method and CDSL
3 Package, CDSL
4 Comparison of operation between conventional method and CDSL Conventional CDSL Day 1 Specimen 5g Specimen 5g Pre-culture Pre-culture (BPW/EEM) (BPW/EEM) Day Selective Enrichment Culture CDSL (TT/RV/SBG/SC) Day 3 Isolation Presume, presence of Salmonella (MLCB/DHL/XLD/Rambach) Isolation Day Presume, presence of Salmonella Confirmation/Identification Confirmation/Identification (Biochemical/Serological Tests) (Biochemical/Serological Tests)
5 Operating procedure of CDSL (1) Pre-culture Medium (BPW,EEM) Sample 5g Pre-incubation - hours at 35 Inoculate.1mL of Pre-culture solution on CDSL (approx. 1cm far from the edge of plate) SL
6 Operating procedure of CDSL () SL Drop 1mL of sterilized water at the opposite point where specimen dropped. Confirm water diffused all over the plate and turn over the plate capped. Incubate hours at 1-3. Interpretation
7 Test Principle and Features of Compact Dry SL Base medium: DHL agar Additives: Pigment / Magnesium chloride / Novobiocin / Other additives Test principle: 1) Medium alkalization that changes medium color from blue-purple to yellow by Salmonella s lysine decarboxylase ability. ) Colony color will be greening caused by decomposition of chromogenic substrate with specific enzyme on Salmonella. 3) Salmonella produces hydrogen (not all strains) sulfide which generate black colonies. ) Detect motility of Salmonella. Features: 1) CDSL detects Salmonella one day earlier than conventional culture methods. ) Isolated colonies on the plate can be fished for further identification tests. 3) Ready to use and portable plate which no need to special preparation.
8 Chicken (, hours incubation) Positive reaction Motility, Yellow sheet, Greening colony, Black colonies are generated by hydrogen sulfide. Negative reaction No color change Red Red purple
9 CDSL reactions (1) Standard strains Table 1: Gram Positive bacteria and Yeast ( hours incubation at ) Tested strains CDSL Growth 1 Bacillus cereus ATCC Bacillus licheniformis ATCC Bacillus subtilis ATCC Corynebacterium minutissium ATCC Corynebacterium renale ATCC Corynebacterium xerosis ATCC Enterococcus avium ATCC Enterococcus durans ATCC Enterococcus faecalis ATCC Enterococcus faecalis NIH Enterococcus faecium ATCC Enterococcus faecium NIH Lactobacillus delbrueckii subsp. Lactis ATCC Micrococcus luteus ATCC Staphylococcus aureus ATCC Staphylococcus epidermidis ATCC Streptococcus thermophilus ATCC Candida albicans ATCC Saccharomyces cerevisiae NHL Color
10 CDSL reactions () Gram Negative Bacteria rather than Salmonella Tested strains Numbers Growth Color Tested strains Numbers Growth Color (NIH, ATCC, JCM) Table : Gram Negative Bacteria rather than Salmone la (NIH, ATCC, JCM) Cedecial davisae - - Klebsiella pneumoniae subsp. rhinoscleromatis -/+ - / purple Cedecia lapageri - - Klebsiella terrigena - - Citrocacter amalonaticus Kluyvera ascorbata Citrobacter diversus 3() -/+ - / yellow Kluyvera clyocrescens Citrobacter freundii 5 -/+ - Leclercia adecarboxylata - - Cirobacter koseri Morganella morganii -/+ - Edwardiella hoshinae - - Proteus mirabilis + - Edwardsiella tarda Proteus penneri - - Enterobacter aerogenes Proteus vulgaris (1) -/+ - / yellow Enterobacter amnigenus Providencia alcalifaciens Enterobacter cloacae 3 + purple Providencia rettgeri 3(1) -/+ - / pale yellow Enterobacter intermidius Providencia stuartii - - Enterobacter sakazakii / purple Pseudomonas aeruginosa 6(5) + - / yellow Escherichia coli / purple Pseudomonas putida 1(1) + yellow Escherichia coli Serotype O157 -/+ - Serratia fonticola Escherichia fergusonii Serratia liquefaciens Escherichia hermannii 3 -/+ - Serratia marcescens -/+ - Escherichia vulneris - - Serratia rubidaea -/+ - Hafnia alvei Rehnella aquatilis Klebsiella ornithinolytica -/+ - Shigella boydii Klebsiella oxytoca Shigella flexneri - - Klebsiella planticola (1) -/+ - / yellow Shigella sonnei - - Klebsiella pneumoniae subsp. Ozaenae 3 + purple Yersinia enterocolitica - - Klebsiella pneumoniae subsp. Pneumoniae / purple Yokenella regensburgei - - Color changed to yellow around sample dropping spot (no green colonies identified) Total number of strains tested 1(11)
11 CDSL reactions (3) Salmonella spp.- 1 Table 3-1: Salmonella spp. ( hours incubation at ) Tested strains Numbers Growth Color (NIH, ATCC, JCM) Inoculated point Surrounding Colonies Salmonella Agona 1 + Yellow Yellow Blue Green Salmonella Anatum + Yellow Yellow/Green Blue Green Salmonella Blockley + Yellow Yellow Blue Green Salmonella bongori 1 + Yellow Gray Blue Green Salmonella Cerro 1 + Yellow/Green Yellow Green Salmonella Chester + Yellow Yellow Green Salmonella Choleraesuis 1 + Yellow Yellow Blue Green Salmonella Cubana, HS- 1 + Yellow Yellow Green Salmonella Derby 1 + Yellow Yellow Blue Green Salmonella enterica subsp. arizonae 1 + Yellow Yellow Blue Green Salmonella enterica subsp. diarizonae 1 + Yellow Gray small colonies Salmonella enterica subsp. houtenae + Yellow Yellow Salmonella Enteritidis 9 + Yellow/Green Yellow Green Salmonella Enteritidis, HS- 5 + Yellow Yellow Green Salmonella Fayed 1 + Yellow Yellow Green Salmonella Hadar 1 + Yellow/Green Yellow Blue Green Salmonella Havana + Yellow/Green Yellow Green Salmonella Heidelberg + Yellow Yellow Blue Green Salmonella Infantis 3 + Yellow Yellow Blue Green/Green 1
12 CDSL reactions (3) Salmonella spp.- Table 3-: Salmonella spp. ( hours incubation at ) Tested strains Numbers Growth Color (NIH, ATCC, JCM) Inoculated point Surrounding Colonies Salmonella Lexington 1 + Yellow Yellow Green Salmonella Livingstone 1 + Yellow Yellow Green Salmonella Manhattan 1 + Yellow Yellow Blue Green Salmonella Mbandaka 1 + Yellow Yellow Blue Green Salmonella Montevideo 1 + Yellow/Green Yellow Blue Green Salmonella Muenchen 1 + Yellow Yellow Green Salmonella Muenster 1 + Yellow Yellow Black Salmonella Newport 1 + Yellow Yellow Blue Green Salmonella Paratyphi B 1 + Yellow Yellow Blue Green Salmonella Schleissheim 1 + Yellow Yellow Blue Green Salmonella Schwarzengrund 1 + Yellow Yellow Blue Green Salmonella Senftenberg 1 + Yellow Yellow Blue Green Salmonella Solna + Yellow/Green Yellow Blue Green Salmonella Thompson 3 + Yellow Yellow Blue Green/Green Salmonella Tshiongwe 1 + Yellow Yellow Green Salmonella Typhi 1 + Yellow Yellow Salmonella Typhimurium 17 + Yellow/Green Yellow Blue Green/Green Salmonella Virchow 1 + Yellow Yellow Green 37
13 Recovery of Salmonella from inoculated Food samples (1) Method Flow Chart Food Sample 5g + Buffer Peptone Water 5mL Food SPC 1. Ground Chicken Breast 5.7 x 1 5 CFU/mL. Dried whole Egg 6. x 1 CFU/mL 3. BPW ---- Dispense 9mL each into sterile test tube Dilute S. Enteritidis (5.1 x 1 8 CFU/mL) from 1-1 to 1-8 Dilute and inoculate 1mL each into above tube Compact Dry SL (Nissui) DIASALM (MERCK) Rappaport-Vassiliadis Broth Inoculation:.1 ml.1 ml.1 ml Incubate hours at C Confirmation of typical Salmonella colonies MLCB Rambach XLD Confirmation: TSI agar / LIM Medium Biochemical Tests: PYR / NPA Serological Test
14 Recovery of Salmonella from inoculated Food samples (1) Table : Recovery of Salmonella from inoculated Food sam ple (Ground Chicken Breast Meat: 5.7 x 1 5 CFU/m L) Inoculated amount of Salmonella (CFU/mL) 5.1 x x x x x x x None CDSL DIASALM W - RV : Potitive ++ : Whole Yellowed Positive +W : Weak Postive Table 5: Recovery of Salmonella from inoculated Food sam ple (Dried Whole Egg : 6. x 1 CFU/m L) Inoculated amount of Salmonella (CFU/mL) 5.1 x x x x x x x None CDSL DIASALM RV : Potitive ++ : Whole Yellowed Positive +W : Weak Postive Table 6: Recovery of Salmonella from inoculated Food sam ple (BPW) Inoculated amount of Salmonella (CFU/mL) 5.1 x x x x x x x None CDSL DIASALM RV : Potitive ++ : Whole Yellowed Positive +W : Weak Postive
15 Recovery of Salmonella from inoculated Food samples () (Sensitivity test for pre-cultured sample) Method Flow Chart Chicken Breast 5g (SPC 8.3 x 1 3 CFU/g) Egg Yolk 5g (SPC < 1CFU/g) Inoculate 1.mL of Pre-cultured Salmonella Typhimurium -7B in TSB (over night) to each food sample (Controlled 1 1 to 1 CFU/mL) Chicken Breast Egg Yolk EEM 5mL (Incubate hours at 35C ) BPW 5mL Inoculate to below media (incubate hours at C ) Compact Dry SL (Nissui) DIASALM (MERCK) Rappaport-Vassiliadis Broth Inoculation:.1 ml.1 ml.1 ml Confirmation of typical Salmonella colonies MLCB Rambach XLD (Incubate hours at 35C ) Confirmation: TSI agar / LIM Medium Biochemical Tests: PYR / NPA Serological Test
16 Recovery of Salmonella from inoculated Food samples () (Sensitivity test from pre-cultured sample) Table 7: Comparison of Salmonella detection from each medium (Chicken Breast meat) Inoculate CDSL DIASALM TT RV (CFU/5g) : Whole reaction are confirmed + : Partial reaction are confirmed Inoculate Table 8: Comparison of Salmonella detection from each medium (Egg Yolk) CDSL DIASALM TT RV (CFU/5g) : Whole reaction are confirmed + : Partial reaction are confirmed
17 Comparison of Salmonella detection by methods from Selective Enrichment Media by meat products (1) Method Flow Chart Food Specimen 5g + EEM Broth 5mL (Meat products, 6 samples) Pre-Enrichment culture < hours at 35C > Compact Dry SL (Nissui) DIASALM (MERCK) Rappaport-Vassiliadis Broth Tetrathionate Broth Inoculation:.1 ml.1 ml.1 ml 1.mL Incubate hours at C MLCB agar / Rambach agar / XLD agar Incubate hours at 35C Confirmation: TSI agar / LIM Medium Biochemical Tests: PYR / NPA Serological Test
18 Comparison of Salmonella detection by methods from Selective Enrichment Media by meat products () Name of Product (selected unintentionally) Number of specimen Compact Dry SL Positives DIASALM Positives RV Positives TT Positives Chicken (breast/ground) Chicken (dark/sliced) Chicken (breast) Chicken (breast white) 6 3 Chicken (leg dark) 6 3 Chicken (ground) Chicken (wing) Chicken (skin) Chicken (gizzard) Chicken (steamed) Bacon Sausage 6 Processed Ham Fried Chicken (Frozen) Japanese Style Fried Chicken (Frozen) TOTAL
19 Comparison of Salmonella detection methods from Selective Enrichment Media by meat products (3) Comparison of Sensitivity, Specificity and Agreement between Compact Dry SL and Conventional method CDSL DIASALM CDSL vs DIASALM Sensitivity 3 / 3 x 1 = 1% Specificity / 6 x 1 = 9.3% Agreement 58 / 6 x 1 = 96.7% CDSL R V CDSL vs RV Broth Sensitivity 3 / 3 x 1 = 1% Specificity / 3 x 1 = 8% Agreement 5 / 6 x 1 = 9% CDSL T T CDSL vs Tetrathionate Broth Sensitivity 3 / 3 x 1 = 1% Specificity / 8 x 1 = 85.7% Agreement 56 / 6 x 1 = 93.3%
20 Conclusion All Salmonella samples are being positive on Compact Dry SL. Several high concentration of Proteus and Pseudomonas strains showed yellowed reaction, but other Gram negatives were negative or inhibited its growth. Sensitivity and specificity of CDSL were higher than conventional methods when detected Salmonella from meat products on the market. Preparation of medium is not necessary for CDSL and the presumption of test result may receive one day earlier than conventional enrichment culture methods that will be useful for food manufacturers.
Bile Chrysoidin Glycerol Agar with MUG
INSTRUCTIONS FOR USE READY-TO-USE PLATED MEDIA PA-257430.04 Rev.: Mar 2013 Bile Chrysoidin Glycerol Agar with MUG INTENDED USE Partially selective medium for the isolation and differentiation of Enterobacteriaceae
More informationAn Inclusivity and Exclusivity Evaluation of the Invisible Sentinel Veriflow Salmonella Species Method for the Detection of Salmonella species
An Inclusivity and Exclusivity Evaluation of the Invisible Sentinel Veriflow Salmonella Species for the Detection of Salmonella species AOAC PTM Evaluation September 20, 2013 Erin S. Crowley, Patrick M.
More information3M Food Safety Technical Bulletin
3M Petrifilm Aqua Enterobacteriaceae Count Plates Performance Summary 3M Petrifi lm Aqua Enterobacteriaceae (AQEB) Count Plates are sample ready media plates used in the microbial testing of bottled water.
More information3M Food Safety Technical Bulletin
3M Petrifilm Aqua Heterotrophic Count Plate Performance Summary 3M Petrifilm Aqua Heterotrophic Count (AQHC) Plates are sample ready media plates used in the microbial testing of bottled water. Each plate
More informationEvaluation of the Modified Micro-ID System for Identification
JOURNAL OF CLINICAL MICROBIOLOGY, Oct. 1979, p. 454-458 0095-1 137/79/10-0454/05$02.00/0 Vol. 10, No. 4 Evaluation of the Modified Micro-ID System for Identification of Enterobacteriaceae WILLIAM J. BUESCHING,'
More informationBD BBL CHROMagar Salmonella* / XLD Agar (Biplate)
PA-257372.02 Page 1 of 7 INSTRUCTIONS FOR USE READY-TO-USE PLATED MEDIA PA-257372.02 Rev.: Mar 2013 BD BBL CHROMagar Salmonella* / XLD Agar (Biplate) * U.S. Patent # 5,098,832, 5,194,374 INTENDED USE BBL
More informationENTEROBACTER AEROGENES UNKNOWN BACTERIA FLOW CHART UNKNOWN LAB REPORT, MICROBIOLOGY ENTEROBACTER AEROGENES
ENTEROBACTER AEROGENES UNKNOWN BACTERIA PDF UNKNOWN LAB REPORT, MICROBIOLOGY ENTEROBACTER AEROGENES IDENTIFICATION OF AN UNKNOWN BACTERIAL SPECIES OF 1 / 5 2 / 5 3 / 5 enterobacter aerogenes unknown bacteria
More informationBIOL 3702L: MICROBIOLOGY LABORATORY SCHEDULE, SUMMER 2015
BIOL 3702L: MICROBIOLOGY LABORATORY SCHEDULE, SUMMER 2015 Week of May 18 th Introduction to the Microbiology Laboratory: Become familiar with the laboratory and its safety features Review safety rules
More informationComparison of rapid cultural methods for the detection of Salmonella species
Zurich Open Repository and Archive University of Zurich Main Library Strickhofstrasse 39 CH-8057 Zurich www.zora.uzh.ch Year: 2013 Comparison of rapid cultural methods for the detection of Salmonella species
More informationCaroline M. O Hara* Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 2006, p. 928 933 Vol. 44, No. 3 0095-1137/06/$08.00 0 doi:10.1128/jcm.44.3.928 933.2006 Evaluation of the Phoenix 100 ID/AST System and NID Panel for Identification
More informationProduct Catalogue 2015 Clinical and Industrial Microbiology
Acinetobacter baumannii ATCC BAA-747 * 89141 Actinomyces odontolyticus ATCC 17929 * 89114 Aeromonas hydrophila ATCC 7966 * 89119 Aggregatibacter aphrophilus ATCC 7901 * 89091 Aspergillus brasiliensis ATCC
More informationSSI ENTERIC PRODUCT INFORMATION. Detects all Enterobacteria. Direct identification. Rapid diagnosis. Cost saving
SSI ENTERIC M E D I U M Detects all Enterobacteria Direct identification Rapid diagnosis Cost saving SSI Diagnostica 2 Herredsvejen 3400 Hillerød Denmark PRODUCT INFORMATION Tel: +45 4829 9100 Fax: +45
More informationID Membranes for Microbial Rapid Identification
ID Membranes for Microbial Rapid Identification Chromogenic Reaction by Specific Substrates on Membranes Classical plates are still used today as first steps in modern microbiology. Smart, inexpensive
More informationProduct Catalogue 2016 Clinical and Industrial Microbiology
Acinetobacter baumannii ATCC BAA-747 * 89141 Acinetobacter baumannii ATCC 19606 * 89174 Actinomyces odontolyticus ATCC 17929 * 89114 Aeromonas hydrophila ATCC 7966 * 89119 Aeromonas hydrophila ATCC 35654
More informationNumerical Diagnostic Key for the Identification of Enterobacteriaceae
APPLIED MICROBIOLOGY, Jan. 1972, p. 108-112 Copyright 0 1972 American Society for Microbiology Vol. 23, No. 1 Printed in U.SA. Numerical Diagnostic Key for the Identification of Enterobacteriaceae HERMAN
More informationSusan Maddocks,* Tom Olma, and Sharon Chen
JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2002, p. 2999 3003 Vol. 40, No. 8 0095-1137/02/$04.00 0 DOI: 10.1128/JCM.40.8.2999 3003.2002 Copyright 2002, American Society for Microbiology. All Rights Reserved.
More informationPharmaceutical Microbiology Forum Newsletter Vol. 12 (4) Page 3 of 14 (NCIMB 8545, CIP NBRC. Salmonella enterica ssp typhimurium
Page 3 of 14 Continued from page 2 Table 2. Absence of Specified Details Media Growth Promotion Organisms for Trypticase Soy Staphylococcus aureus Escherichia coli Pseudomonas aeruginosa Salmonella Staphylococcus
More informationCERTIFICATION. Certificate No. The AOAC Research Institute hereby certifies that the performance of the test kits known as:
CERTIFICATION AOAC Performance Tested SM Certificate No. 120301 The AOAC Research Institute hereby certifies that the performance of the test kits known as: foodproof Salmonella Detection Kits (liquid
More informationD-10 VALUES OF MICRO-ORGANISMS
INFORMATION SHEET SYNERGY HEALTH D-10 VALUES OF MICRO-ORGANISMS Synergy Health Applied Sterilisation Technologies (AST) operates as a global supplier of sterilisation, specialist applications and contamination
More informationMicrobial Typing by Machine Learned DNA Melt Signatures
Microbial Typing by Machine Learned DNA Melt Signatures Nadya Andini 1, Bo Wang 2, Pornpat Athamanolap 3, Justin Hardick 4, Billie J. Masek 5, Simone Thair 1, Annie Hu 1, Gideon Avornu 5, Stephen Peterson
More informationOur product offering. The ATCC Licensed Derivative Program
Our product offering Currently MECCONTI offers three different product lines of lyophilized micro-organisms: MicroSwabs: A MicroSwab consists of a lyophilized pellet of a single micro-organism strain inside
More informationEZ-COMP EZ-COMP For Training and Proficiency Testing Product Details
EZ-COMP For Training and Proficiency Testing Mixed microorganism populations Identified by codes rather than descriptions Refrigerated storage Traceable to reference culture Product warranty Product Details
More informationEvaluation of the Quantitative Dry Culture Method Sanitakun TM SA for the Enumeration of Staphylococcus aureus
Biocontrol Science, 2015, Vol. 20, No. 4, 297 301 Note Evaluation of the Quantitative Dry Culture Method Sanitakun TM SA for the Enumeration of Staphylococcus aureus in Artificially Contaminated Food Samples
More informationEvaluation of Mast-ID 15 system for identifying
J Clin Pathol 7;4:6-73 Evaluation of Mast-ID 5 system for identifying Enterobacteriaceae, some Vibrionaceae, and Acinetobacter B HOLMES, C A DAWSON From the National Collection of Type Cultures, Central
More informationToronto General Hospital ANTIBIOGRAM Emergency Department January 1, December 31, 2016
IV (meningitis) IV (non-meningitis) (meningitis) (non-meningitis) Blood Isolates % Susceptible 644 18 36 70 78 74 59 69 75 262 100 19 64 75 100 92 54 72 78 76 68 89 86 99 Escherichia coli 153 58 30 67
More informationPATHfinder. User Manual. PATHfinder Salmonella spp. Assay. Real-Time PCR kit for Salmonella spp. detection. Cat. # PMB01A/PMB01A-50
PATHfinder PATHfinder Salmonella spp. Assay Real-Time PCR kit for Salmonella spp. detection Cat. # PMB01A/PMB01A-50 User Manual Via San Geminiano, 4 41030 San Prospero (MO)- Italy : +39 059 8637161 : +39
More informationCERTIFICATION. Certificate No. The AOAC Research Institute hereby certifies that the performance of the test kit known as: E. coli O157:H7 Assay Kit
CERTIFICATION AOAC Performance Tested SM Certificate No. 100901 The AOAC Research Institute hereby certifies that the performance of the test kit known as: E. coli O157:H7 Assay Kit manufactured by BioFire
More informationGentamicin Rationale for the EUCAST clinical breakpoints, version th February, 2009
Gentamicin Rationale for the EUCAST clinical breakpoints, version 1.2 16 th February, 2009 Introduction The aminoglycosides are a group of naturally occurring or semi-synthetic compounds with bactericidal
More informationEvaluation of a new chromogenic selective medium for isolation and enumeration of Vibrio parahaemolyticus
African Journal of Microbiology Research Vol. 5(21), pp. 3432-3436, 9 October, 2011 Available online http://www.academicjournals.org/ajmr ISSN 1996-0808 2011 Academic Journals Full Length Research Paper
More informationFood Science Immune Sera. Bacterial Serotyping Guide for Salmonella
Food Science Immune Sera Bacterial Serotyping Guide for Salmonella Minimizing Risk The monitoring of veterinary diseases and quality control of industrial products are public health issues. Microbial populations
More informationMost common dose (mg) 1g x 1 1g x 1 1g x 1 1g x 1 1g x 1 1g x 1. Maximum dose schedule (mg) 1g x 1 1g x 1 1g x 1 1g x 1 1g x 1 1g x 1
Ertapenem Rationale for the EUCAST clinical breakpoints, version 1.3 1 st June 2009 Introduction Ertapenem is a carbapenem, available only for parenteral use. Ertapenem is relevant for therapy of septicaemia,
More informationNF VALIDATION Validation of alternative analytical methods Application in food microbiology. Summary report
ACCREDITATION N 1-0144 PORTEE DISPONIBLE SUR WWW.COFRAC.FR 3M Boulevard de l Oise F-95006 CERGY PONTOISE NF VALIDATION Validation of alternative analytical methods Application in food microbiology Summary
More informationDevelopment of an improved selective and differential medium. for the isolation of Salmonella spp.
JCM Accepts, published online ahead of print on 18 July 2012 J. Clin. Microbiol. doi:10.1128/jcm.01228-12 Copyright 2012, American Society for Microbiology. All Rights Reserved. 1 2 3 4 5 6 7 8 9 10 11
More informationof the Family Enterobacteriaceae
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, Apr. 1995, p. 5261 00207713/ 95/$04.00 0 Copyright 0 1995, International Union of Microbiological Societies Vol. 45, No. 2 Heterogeneous Patterns of Acid
More informationMost common dose (mg) 1 g x 3 1 g x mg -1.0 g x 3 1 g x mg -1 g x mg -1 g x 3
Meropenem Rationale for the EUCAST clinical breakpoints, version 1.5 1 st June 2009 Introduction Meropenem is a carbapenem, available only for parenteral use. Meropenem is relevant for therapy of septicaemia,
More informationSULFIDE-INDOLE-MOTILITY (SIM) TEST
Microbiology Laboratory (BIOL 3702L) Page 1 of 5 Principle and Purpose SULFIDE-INDOLE-MOTILITY (SIM) TEST Using Sulfide-Indole-Motility (SIM) media, various Gram-negative enteric bacilli can be distinguished
More informationOverview of the major bacterial pathogens The major bacterial pathogens are presented in this table:
Practical Microbiology 30/11/2018 University of Sulaimani college of Pharmacy Year2 Lab. 5: Overview of the major bacterial pathogens The major bacterial pathogens are presented in this table: Major Bacterial
More informationMicrobial pollutants in stagnant water in an informal settlement in the Western Cape, South Africa
Microbial pollutants in stagnant water in an informal settlement in the Western Cape, South Africa Qenehelo A. Leuta, James P. Odendaal and Arnelia N. Paulse Department of Environmental and Occupational
More informationLaboratory Exercise # 7: Aseptic Technique
Laboratory Exercise # 7: Aseptic Technique Purpose: The purpose of this laboratory exercise is to acquaint the student with the procedures of aseptic transfer of microbiological cultures. ntroduction:
More informationEUCAST Expert Rules Version 3.1. Intrinsic Resistance and Exceptional Phenotypes Tables
EUCAST Expert s Version 3.1 Intrinsic Resistance and Exceptional Phenotypes Tables EUCAST Expert s version 2.0 was published on 29 October 2011(http://www.eucast.org/expert_rules). The expert rules have
More informationAOAC SMPR Standard Method Performance Requirements for Detection of Salmonella species in Romaine Lettuce and Baby Spinach
AOAC SMPR 2014.017 Standard Method Performance Requirements for Detection of Salmonella species in Romaine Lettuce and Baby Spinach Intended Use: Routine Surveillance and Monitoring by a Trained Technician
More informationNUT-TTC/EMB Code 5541
NUT-TTC/EMB Code 5541 COMING SOON! BioPaddles Colony Identification App Nutrient-TTC Agar (NUT-TTC) Eosin Methylene Blue Agar (EMB) USE: Isolation and differentiation of Gram (-) enteric bacilli. Coliform
More information_ + Discriminates aerobic organisms that produce catalase to degrade hydrogen peroxide into water and oxygen
Lab 11 Goals and Objectives: Catalase Test Exercise 39: Oxidation and Fermentation Tests (Catalase) Exercise 67: Staphylococci Identification (MSA & Coagulase) Exercise 68: Streptococci & Enterococci Identification
More informationThermo Scientific RapidFinder Salmonella species, Typhimurium, and Enteritidis PCR Kit AOAC- RI PTM Matrix Extension: Method Comparison Study
STUDY REPORT Thermo Scientific RapidFinder Salmonella species, Typhimurium, and Enteritidis PCR Kit AOAC- RI PTM Matrix Extension: Method Comparison Study Jessica Williams Thermo Fisher Scientific, Wade
More informationCATALOGUE OF TEST, CONTROL OR BIOASSAY STRAINS FROM BCCM CULTURE COLLECTIONS
CATALOGUE OF TEST, CONTROL OR BIOASSAY STRAINS FROM BCCM CULTURE COLLECTIONS LIST OF STRAINS INVOLVED IN NORMS AND STANDARDISED MICROBIOLOGICAL METHODS The Belgian Co-ordinated Collections of Micro-organisms
More informationKIYOFUMI OHKUSU* Department of Clinical Laboratory, Chiba Children s Hospital, Chiba, , Japan
JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 2000, p.4586 4592 Vol. 38, No. 12 0095-1137/00/$04.00 0 Copyright 2000, American Society for Microbiology. All Rights Reserved. Cost-Effective and Rapid Presumptive
More informationTetracycline Rationale for the EUCAST clinical breakpoints, version th November 2009
Tetracycline Rationale for the EUCAST clinical breakpoints, version 1.0 20 th November 2009 Introduction The natural tetracyclines, including tetracycline, chlortetracycline, oxytetracycline and demethylchlortetracycline
More informationSystem in Comparison with the API 20E System
JOURNAL OF CLINICAL MICROBIOLOGY, July 983, p. 2835 Vol. 8, No. 009537/83/0702808$02.00/0 Copyright C 983, American Society for Microbiology Evaluation of the Updated MS2 Bacterial Identification System
More informationRetail Catalog 39th Edition Supplemental Information Individual Microorganisms Price Microorganism Description
LYFO DISK KWIK-STIK Vial 6 Pk Pk Individual Microorganisms Price Microorganism Description ode BSL omment 049L 049K 049P 0645L 0645K 0645P Aggregatibacter aphrophilus AT 4997 D formerly Haemophilus paraphrophilus
More informationIdentification of Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the VITEK MS system
DOI 10.1007/s10096-013-1912-y ARTICLE Identification of Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the VITEK MS system S. S. Richter & L. Sercia
More informationMulticenter Evaluation of the MicroScan Rapid Gram-Negative Identification Type 3 Panel
JOURNAL OF CLINICAL MICROBIOLOGY, Oct. 1997, p. 2531 2536 Vol. 35, No. 10 0095-1137/97/$04.00 0 Copyright 1997, American Society for Microbiology Multicenter Evaluation of the MicroScan Rapid Gram-Negative
More informationClinical Laboratory Evaluation of the AutoMicrobic System
JOURNAL OF CLINICAL MICROBIOLOGY, OCt. 1981, p. 370-375 0095-1 137/81/100370-06$02.00/0 Vol. 14, No. 4 Clinical Laboratory Evaluation of the AutoMicrobic System Enterobacteriaceae Biochemical Card JAMES
More informationMulticenter Comparison of In Vitro Activities of FK-037, Cefepime, Ceftriaxone, Ceftazidime, and Cefuroxime
ANTIMICROBiAL AGENTS AND CHEMOTHERAPY, Aug. 3, p. 6-66-/93/6-5$./ Copyright 3, American Society for Microbiology Vol. 3, No. Multicenter Comparison of In Vitro Activities of,,,, and J. A. WASHINGTON,"*
More informationGram negative bacilli
Gram negative bacilli 1-Enterobacteriaceae Gram negative bacilli-rods Enterobacteriaceae Are everywhere Part of normal flora of humans and most animals They are cause of -30-35% septisemia -more than 70%
More informationTransia Plate Salmonella Gold for the detection of Salmonella spp. in foods and feeds
Transia Plate Salmonella Gold for the detection of Salmonella spp. in foods and feeds Abstract A method for the detection of Salmonella spp. in foods and feeds is presented. This method, which is based
More informationThe Attenuated Total Reflectance Infrared Microspectroscopy. of Bacterial Colonies on Agar Plates. An Honors Thesis (HONRS 499) Jennifer L.
The Attenuated Total Reflectance Infrared Microspectroscopy of Bacterial Colonies on Agar Plates An Honors Thesis (HONRS 499) By Jennifer L. Hodges Thesis Advisor Patricia L. Lang Ball State University
More informationStability. Received for publication 1 August to be fl-lactamase-producing strains.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Apr. 1978, p. 584-588 0066-4804/78/0013-0584$02.00/0 Copyright X) 1978 American Society for Microbiology Vol. 13, No. 4 Printed in U.S.A. Cefaclor: In Vitro Spectrum
More informationANTIMICROBIAL TESTING. E-Coli K-12 - E-Coli 0157:H7. Salmonella Enterica Servoar Typhimurium LT2 Enterococcus Faecalis
ANTIMICROBIAL TESTING E-Coli K-12 - E-Coli 0157:H7 Salmonella Enterica Servoar Typhimurium LT2 Enterococcus Faecalis Staphylococcus Aureus (Staph Infection MRSA) Streptococcus Pyrogenes Anti Bacteria effect
More informationEN ISO validation study of the Assurance GDS TM Salmonella method
BIOCONTROL SYSTEMS Inc. 12822 SE 32nd Street BELLEVUE, WA 98005 USA NF VALIDATION Validation study according to the EN ISO 16140 standard: Summary Report EN ISO 16140 validation study of the Assurance
More informationClassification of Enterobacteria Based on Overall
JOURNAL OF BAcTERIOLOGY, Nov., 1966 Copyright 1966 American Society for Microbiology Vol. 92, No. 5 Printed In U.S.A. Classification of Enterobacteria Based on Overall Similarity1 R. E. KRIEG AND W. R.
More informationInfluence of Food Microorganisms on Staphylococcal Growth and Enterotoxin Production in Meat
APPLIED MICROBIOLOGY, May, 1966 Copyright 1966 American Society for Microbiology Vol. 14, No. 3 Printed in U.S.A. Influence of Food Microorganisms on Staphylococcal Growth and Enterotoxin Production in
More informationANTIBACTERIAL ACTIVITY OF PLANT EXTRACTS IN FOOD PRODUCTS
ANTIBACTERIAL ACTIVITY OF PLANT EXTRACTS IN FOOD PRODUCTS Antanas Šarkinas Food institute of Kaunas University of Technology, Taikos pr. 92, LT-51180, Kaunas; direktorius@lmai.lt Spices Spices have been
More informationLibrary Stats Sheet: Gram-negative
Library Stats Sheet: Gram-negative Not for Use in Diagnostic Procedures. DiversiLab Kit: Bacterial (cat.# 270633) DiversiLab Library Name: DL Gram-negative Library Application: Strain-typing for all Gram-negative
More informationcolony size color morphology haemolysis S. aureus S. epidermidis
practical 2.: STAPHYLOCOCCUS 1. Prepare a heat fixed smear of the culture of S.aureus. (Gram staining, microscopy). 2. Prepare a heat fixed smear of the culture of S.aureus. and S.epidermidis (mixed smear),
More informationSystem with a Conventional Broth System
JOURNAL OF CLINICAL MICROBIOLOGY, Sept. 98, p. 55-5 95-7/8/955-6$./ Copyright C 98, American Society for Microbiology Vol. 6, No. Clinical Laboratory Comparison of a Slide Blood Culture System with a Conventional
More information3M Food Safety 3M Petrifilm Salmonella Express System
3M Petrifilm Salmonella Express System 2 3M Food Safety 3M Petrifilm Salmonella Express System 3M Petrifilm Salmonella Express System is a qualitative test used for the rapid detection and biochemical
More informationCHN62: REPORTING OF MICROBIOLOGY RESULTS
CHN62: 1.1 Introduction This SOP provides guidance on reporting of all the microbiological results in the Microbiology laboratory for CHAIN study. 1.2 Purpose This SOP will aid in a standard reporting
More informationEvaluation of the VITEK 2 System for Rapid Identification of Medically Relevant Gram-Negative Rods
JOURNAL OF CLINICAL MICROBIOLOGY, July 1998, p. 1948 1952 Vol. 36, No. 7 0095-1137/98/$04.00 0 Copyright 1998, American Society for Microbiology. All Rights Reserved. Evaluation of the VITEK 2 System for
More informationCERTIFICATION. Certificate No. The AOAC Research Institute hereby certifies that the performance of the test kit known as:
CERTIFICATION AOAC Performance Tested SM Certificate No. 100201 The AOAC Research Institute hereby certifies that the performance of the test kit known as: BAX System PCR Assay for Salmonella BAX System
More informationComparison of Crystal Enteric/Nonfermenter System, API 20E System, and Vitek Automicrobic System for Identification of Gram-Negative Bacilli
JOURNAL OF CLINICAL MICROBIOLOGY, Feb. 1995, p. 364 370 Vol. 33, No. 2 0095-1137/95/$04.00 0 Copyright 1995, American Society for Microbiology Comparison of Crystal Enteric/Nonfermenter System, API 20E
More informationINTERPRETATION OF THE GRAM STAIN
INTERPRETATION OF THE GRAM STAIN DISCLOSURE Relevant relationships with commercial entities none Potential for conflicts of interest within this presentation none Steps taken to review and mitigate potential
More informationDesign of an Enterobacteriaceae Pan-genome Microarray Chip
Design of an Enterobacteriaceae Pan-genome Microarray Chip Oksana Lukjancenko and David W. Ussery DTU CBS 2010 2 Background Pan-genome complete collection of variuos genes located within populations at
More informationEvaluation of the efficiency of Mxxxx as a barrier against microrganisms crossing
Evaluation of the efficiency of as a barrier against microrganisms crossing A) composition of filter The filter of has the following characteristics: 1. An outer layer, which is composed by a medical,
More informationSupporting Information
1 Supporting Information 2 3 4 5 Automated High-Throughput Identification and Characterisation of Clinically Important Bacteria and Fungi using Rapid Evaporative Ionisation Mass Spectrometry (REIMS) 6
More informationparatyphi. Second, a systematic comparison of media developed in recent years. Among investigations in which this
A COMPARISON OF COBALT AND NICKEL SALTS WITH OTHER AGENTS FOR THE DETECTION OF HY- DROGEN SULFIDE IN BACTERIAL CULTURES WILLIAM P. UTERMOHLEN, JR.1 AND CARL E. GEORGI Departments of Chemistry and Bacteriology,
More informationNitroxoline Rationale for the NAK clinical breakpoints, version th October 2013
Nitroxoline Rationale for the NAK clinical breakpoints, version 1.0 4 th October 2013 Foreword NAK The German Antimicrobial Susceptibility Testing Committee (NAK - Nationales Antibiotika-Sensitivitätstest
More informationTHE IDENTIFICATION OF TWO UNKNOWN BACTERIA AFUA WILLIAMS BIO 3302 TEST TUBE 3 PROF. N. HAQUE 5/14/18
THE IDENTIFICATION OF TWO UNKNOWN BACTERIA AFUA WILLIAMS BIO 3302 TEST TUBE 3 PROF. N. HAQUE Introduction: The identification of bacteria is important in order for us to differentiate one microorganism
More informationCERTIFICATION. Certificate No. The AOAC Research Institute hereby certifies that the performance of the test kit known as: Solus One Salmonella
CERTIFICATION AOAC Performance Tested SM Certificate No. 101801 The AOAC Research Institute hereby certifies that the performance of the test kit known as: manufactured by Solus Scientific Ltd. Unit 9
More informationCERTIFICATION. Certificate No. The AOAC Research Institute hereby certifies that the performance of the test kit known as:
CERTIFICATION AOAC Performance Tested SM Certificate No. 8121 The AOAC Research Institute hereby certifies that the performance of the test kit known as: BAX System Real Time PCR Assay for Salmonella manufactured
More informationμ gyra parc Escherichia coli Klebsiella pneumoniae Pseudomonas aeruginosa E. coli gyra E. coli parc gyra parc gyra Escherichia coli E. coli E.
gyra parc Escherichia coli Klebsiella pneumoniae Pseudomonas aeruginosa E. coli μ E. coli gyra parc gyra parc gyra parc μ μ gyra parc Key words Escherichia coli gyra parc Escherichia coli E. coli gyra
More informationNF VALIDATION Validation of alternative analytical methods Application in food microbiology. Summary report
ACCREDITATION N 1-0144 PORTEE DISPONIBLE SUR WWW.COFRAC.FR THERMO FISHER SCIENTIFIC 2130 Woodward Austin, TX 78744 USA NF VALIDATION Validation of alternative analytical methods Application in food microbiology
More informationITALY TRENDS AND SOURCES OF ZOONOSES AND ZOONOTIC AGENTS IN HUMANS, FOODSTUFFS, ANIMALS AND FEEDINGSTUFFS
ITALY The Report referred to in Article 9 of Directive 2003/99/EC TRENDS AND SOURCES OF ZOONOSES AND ZOONOTIC AGENTS IN HUMANS, FOODSTUFFS, ANIMALS AND FEEDINGSTUFFS including information on foodborne
More informationDong-Hyun Kang, Daniel Y.C. Fung
International Journal of Food Microbiology 54 (2000) 127 132 www.elsevier.nl/ locate/ ijfoodmicro Short communication Application of thin agar layer method for recovery of injured Salmonella typhimurium
More informationPhylogenetic Diversity of Coliform Isolates in USA. Phylogenetic Classification
Phylogenetic Diversity of Coliform Isolates in USA Ya Zhang and Wen Tso Liu University of Illinois at Urbana Champaign Mark LeChevallier American Water Inc. Nov 2011 Phylogenetic Classification group organisms
More informationPerformances of VITEK 2 Colorimetric Cards for Identification of Gram-Positive and Gram-Negative Bacteria
JOURNAL OF CLINICAL MICROBIOLOGY, Sept. 2005, p. 4402 4406 Vol. 43, No. 9 0095-1137/05/$08.00 0 doi:10.1128/jcm.43.9.4402 4406.2005 Copyright 2005, American Society for Microbiology. All Rights Reserved.
More informationComparison of 61 E. coli genomes
Comparison of 61 E. coli genomes Center for Biological Sequence Analysis Department of Systems Biology Dave Ussery! DTU course 27105 - Comparative Genomics Oksana s 61 E. coli genomes paper! Monday, 23
More informationQuality Control Solutions for Food Testing
Quality Control Solutions for Food Testing QUALITY CONTROL SOLUTIONS FOR FOOD TESTING ATCC offers an expanding portfolio of quality control products to ensure the accuracy and reliability of your food
More informationCell Shape coccus bacillus spirillum vibrio
wrong 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 right 56 55 54 53 52 51 50 49 48 47 46 45 44 43 42 41 40 39 38 score 100 98.2 96.4 94.6 92.9 91.1 89.3 87.5 85.7 83.9 82.1 80.4 78.6 76.8 75 73.2 71.4
More informationUpdate on the ISBT TTID study on establishment of bacterial reference strains for RBC
Update on the ISBT TTID study on establishment of bacterial reference strains for RBC Dr. Marcel Prax Paul-Ehrlich-Institut Federal Institute for Vaccines and Biomedicines Division Microbial Safety Germany
More informationApplied Veterinary Bacteriology and Mycology: Identification of aerobic and facultative anaerobic bacteria Chapter 2: The Enterobacteriaceae
Applied Veterinary Bacteriology and Mycology: Identification of aerobic and facultative anaerobic bacteria Author: Mnr J.J. Gouws Licensed under a Creative Commons Attribution license. TABLE OF CONTENTS
More informationConcentrated Milk. Wisconsin, Madison, Wisconsin data generated by this study would enable the
APPLIED MIcRosaoLOGY, Feb. 1972, p. 415-420 Vol. 23, No. 2 Copyright @ 1972 American Society for Microbiology Printed in USA. Heat Resistance of Salmonellae in Concentrated Milk C. A. DEGA, J. M. GOEPFERT,
More informationSTUDY OF FREQUENCY OF SALMONELLA STRAINS ISOLATED FROM MEAT, MEAT PRODUCTS AND ORGANS
STUDY OF FREQUENCY OF SALMONELLA STRAINS ISOLATED FROM MEAT, MEAT PRODUCTS AND ORGANS CARMEN DAVID 2, R. TRIF 1, E. TÎRZIU 1, ROXANA IRIMESCU 1, R. V. GROS 1 1 - Faculty of Veterinary Medicine, Timisoara,
More informationPREDICTIVE MICROBIOLOGICAL MODELS: WHAT ARE THEY AND HOW CAN THEY BE USED IN THE FOOD INDUSTRY?
PREDICTIVE MICROBIOLOGICAL MODELS: WHAT ARE THEY AND HOW CAN THEY BE USED IN THE FOOD INDUSTRY? WHY USE MODELS? Predictive microbiological models are tools that can be used to assess product shelf-life
More informationSEL, a Selective Enrichment Broth for Simultaneous Growth of. Listeria monocytogenes
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Aug. 2008, p. 4853 4866 Vol. 74, No. 15 0099-2240/08/$08.00 0 doi:10.1128/aem.02756-07 Copyright 2008, American Society for Microbiology. All Rights Reserved. SEL,
More informationEvaluation of a novel selective medium for isolation of Staphylococcus lugdunensis from wound specimens
JCM Accepts, published online ahead of print on 23 April 2014 J. Clin. Microbiol. doi:10.1128/jcm.00706-14 Copyright 2014, American Society for Microbiology. All Rights Reserved. 1 2 3 4 5 6 JCM00706-14
More informationCONTROL ORGANISMS ss
CONTROL ORGANISMS 032918ss CONTENTS CONTROL ORGANISMS 1 Lyophilized KWIK-STIK & LYFO DISK 29 Epower 32 Epower CRM 34 EZ-Accu Shot 36 EZ-Accu Shot Select 36 EZ-CFU 38 EZ-CFU One Step 41 EZ-Hydro Shot 40
More informationidentification system
J Clin Pathol 1988;41:910-914 Evaluation of the Microbact-24E bacterial identification system JULIA M LING, Y-W HUT, G L FRENCH Department ofmicrobiology, The Chinese University of Hong Kong, The Prince
More informationWHO Global Foodborne Infections Network (formerly WHO Global Salm-Surv)
WHO Global Foodborne Infections Network (formerly WHO Global Salm-Surv) "A WHO network building capacity to detect, control and prevent foodborne and other enteric infections from farm to table Laboratory
More informationProject Title: Estimation of the area affected by animal feces in vegetable field under overhead sprinkle irrigation system
I. Abstract. Project Title: Estimation of the area affected by animal feces in vegetable field under overhead sprinkle irrigation system Project Investigator(s): Jorge M. Fonseca 1, Sadhana Ravishankar
More informationUse of the 3M Molecular Detection System for Salmonella and Listeria spp.
Use of the 3M Molecular Detection System for Salmonella and Listeria spp. March 11, 213 Prof Steve Forsythe Pathogen Research Centre, School of Science and Technology Nottingham Trent University Clifton
More information