Analytical Ultracentrifugation Macromolecule Characterization in Solution
|
|
- Loraine Wiggins
- 6 years ago
- Views:
Transcription
1 Analytical Ultracentrifugation Macromolecule Characterization in Solution
2 AUC TECH OVERVIEW OPTIMA AUC Analytical Ultracentrifugation Beckman Coulter delivered the first AUC sample characterization tool to the scientific community powering discoveries. The tradition continues into the 21st century with the new Optima AUC system. This latest offering is the most robust technology for providing protein molecular weight in basic protein research and quantification of aggregation levels for academic and biopharma research. Analytical ultracentrifugation is the most versatile, rigorous and accurate means for determining the molecular weight, hydrodynamic and thermodynamic properties of a protein or other macromolecule. Currently, there is no other technique capable of providing the same range of information with a comparable level of precision and accuracy. AUC Applications Molecular Weight Stoichiometry Protein Aggregation Ligand Binding Conjugation efficiency Polydispersity For the first time, these advanced features enable revolutionary new multi-wavelength experiments, an entirely new class of experimental designs that can exploit the presence of multiple chromophores in complex mixtures through spectral decomposition. Borries Demeler (University Texas Health Science Center) Brilliance at Every Turn
3 Advantages Sample recovery No dilution required Matrix free Minimal buffer constraints Detection at low concentrations Low sample volume (0.1 ml minimum) High throughput No standards required The Optima AUC combines the power of a centrifuge to provide sedimentation of particles and the functionality of an optical module to detect the sedimentation over time. AUC technology provides insight into sample molecular weight, shape, conformation, and heterogeneity. It was 90 years ago that the analytical ultracentrifuge became the world s best, most natural way of characterizing proteins. Now, our newly upgraded Optima AUC offers absorbance scans in seven seconds and interference scans in less than 5 seconds. The advanced optics simplify cleaning and produce more valid, reproducible data.
4 DON T CALL IT A COMEBACK 1920s 1940s 1950s 1960s 1970s 1990s 2000s 2010s Optima AUC is introduced opening up the possibilities for future discoveries Development of flotation Sedimentation Techniques ProteomeLab XL-A and XL-I are introduced by Beckman Coulter The Optima XL-A and XL-I centrifuges introduced by Beckman Coulter β Chain α Chain Rayleigh interference optics developed in Schachman s Laboratory, giving the ability to characterize particles with low absorption in range outside of the visible UV spectrum. Beckman Instruments acquires Spinco (1954) Plasma and blood volume extender methods for transfusions developed 1925: The Analytical Ultracentrifuge is invented by Theodor Svedberg The AN 60 Rotor introduced by Beckman Coulter Heme group Discovery of semi-conservative replication of DNA by Meselson and Stahl Ability to determine size, shape, density, and electric charge β Chain α Chain Tertiary and Quaternary Structure established Original Parent Molecule Discovery of Johnston-Ogston effect and the resultant boundary anomaly Establish basis of protein science and molecular biology First Generation Daughter Molecules Second Generation Daughter Molecules Due to the large size and conformational heterogeneity of our samples, sedimentation velocity experiments in the analytical ultracentrifuge have allowed us to make many seminal findings about chromatin condensation mechanisms that would not have been possible with any other technique. The first commercially available centrifuge, the Spinco Model E, is introduced (1947) Lamm Equation is created, describing the sedimentation and diffusion of a solute under ultracentrifugation. Jeffrey C. Hansen, PhD, Colorado State University Brilliance at Every Turn
5 PRODUCT SPECIFICATION COMPARISON ProteomeLab XL-I Optima AUC** Optical Systems Fastest Data Acquisition Rate ABS: 90 sec/cell INT: 5 sec/scan ABS: <7 sec/sector* INT: <5 sec/scan Max # of Wavelengths 3 20** Wavelength Precision +/- 3 nm +/- 0.5 nm Lowest Radial Resolution 30 µm 10 µm Absorbance Flash Lamp Frequency 50 Hz 300 Hz CCD Camera Specifications 2048 x 96 pixels 2048 x 1088 pixels Interference Fringes 4 fringes/cell 10 fringes/cell Usable Concentration Ranges Hardware Operating System Remote Experimental Setup, Monitoring, and Data Export ABS: mg/ml INT: mg/ml Windows No ABS:.005 ~1-2 mg/ml Luteinizing Hormone INT: ~4-5 mg/ml BSA LINUX Remote GUI (any operating system) Yes Display 7 inches 15 inches Sample Temperature Control Accuracy +/-0.5 C +/- 0.5 C*** Sample Temperature Range 4-40 C 0-40 C**** Optical Architecture Dependent systems Open, Modular (up to 3 detection capabilities at once) *At optimal rotor speeds, at 10 µm radial resolution **Product under development specifications pending system verification ***After reaching temperature equilibrium ***Environmental temperature must be less than 25 C to reach below 4 C
6 INSTRUMENTS & COMPONENTS Ordering Information Component Part No. Description Optima AUC - Absorbance Only B86438 Hardware only with Absorbance optics Optima AUC - ABS/INT B86437 Hardware only with Absorbance + Interference optics Optima AUC ABS Bundle + An-60 C00707 Optima AUC (A) + Starter Kit (1 CB, 1 torque stand, 1 Quartz Cell + 1 accessory kit) + An-60 Optima AUC ABS/ INT Bundle + An-60 C00708 Optima AUC (A/I) + Starter Kit (1 CB, 1 torque stand, 1 Quartz Cell + 1 accessory kit) + An-60 Optima AUC ABS Bundle + An-50 C00709 Optima AUC (A) + Starter Kit (1 CB, 1 torque stand, 1 Quartz Cell + 1 accessory kit) + An-50 Optima AUC ABS/ INT Bundle + An-50 C00710 Optima AUC (A/I) + Starter Kit (1 CB, 1 torque stand, 1 Quartz Cell + 1 accessory kit) + An-50 An-60 rotor hole rotor rated to 60,000 rpm An-50 rotor hole rotor rated to 50,000 rpm 2-sector charcoal Quartz SV cell with charcoal flow-through centerpiece and quartz windows Torque stand Assembly Required to torque cells to prevent sample leaking Counterbalance Kit counterbalance with mask + weights Accessory Kit brass housing plugs Cell housing kit window assemblies + cell housing 2016 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo and the Beckman Coulter product and service names mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. For Beckman Coulter s worldwide office locations and phone numbers, please visit Contact Us at beckman.com CENT-1631SB04.16-A
THE SOLUTION CONFORMATION OF NOVEL ANTIBODY FRAGMENTS STUDIED USING THE PROTEOMELAB XL-A ANALYTICAL ULTRACENTRIFUGE
APPLICATION INFORMATION Peter J. Morgan, Olwyn D. Byron, Stephen E. Harding Department of Applied Biochemistry and Food Science University of Nottingham Sutton Bonington, U. K. Introduction One of the
More informationa-chymotrypsin: CHARACTERIZATION OF A SELF-ASSOCIATING SYSTEM IN THE ANALYTICAL ULTRACENTRIFUGE
APPLICATION INFORMATION Paul Voelker and Don McRorie Beckman Coulter Introduction a-chymotrypsin: CHARACTERIZATION OF A SELF-ASSOCIATING SYSTEM IN THE ANALYTICAL ULTRACENTRIFUGE We have studied the dimerization
More informationBy: Ashley and Christine Phy 200 Professor Newman 4/13/12
By: Ashley and Christine Phy 200 Professor Newman 4/13/12 What is it? Technique used to settle particles in solution against the barrier using centrifugal acceleration Two Types of Centrifuges Analytical
More informationExperimental Design and Data Collection
A. Checks to run to make sure the instrument is in good working condition B. Decide which experiment type is most appropriate, equilibrium or velocity C. Buffer considerations D. Speed selection and length
More informationUltraScan Workshop/Bioc5083 Hydrodynamic Methods
UltraScan Workshop/Bioc5083 Hydrodynamic Methods Borries Demeler, Ph.D. Department of Biochemistry May/June 2013 Analytical Ultracentrifugation Background What can be learned from AUC? Excellent method
More informationMeet Stunner: The one-shot protein concentration and sizing combo
TECH NOTE Meet Stunner: The one-shot protein concentration and sizing combo Introduction What if you could get a better read on the quality of your biologics and use less sample at the same time? Stunner
More informationSupporting Information
Supporting Information Band Sedimentation Experiment in Analytical Ultracentrifugation Revisited Cornelia M. Schneider, Dirk Haffke, Helmut Cölfen* Physical Chemistry, University of Konstanz, Universitätsstrasse
More information2 Analytical Ultracentrifugation, Instrumentation
2 Analytical Ultracentrifugation, Instrumentation Centrifugation is a common technical process for the separation of materials consisting of two (or more) compounds having different sizes and/or showing
More informationJoint use of SAS and AUC
Joint use of SAS and AUC Olwyn Byron School of Life Sciences College of Medical, Veterinary and Life Sciences University of Glasgow, Scotland UK Outline Principles underlying AUC How AUC experiments are
More informationEquilibrium Sedimentation & Sedimentation Velocity: Random Walks in the presence of forces.
New Posts at the Course Website: Origin Assignment 3 on Analytical Ultracentrifugation (due 3/2/12) Problem Set Data (xls file) Resource Booklet (R7) on Analytical Ultracentrifugation Equilibrium Sedimentation
More informationUltra-fast determination of base metals in geochemical samples using the 5100 SVDV ICP-OES
Ultra-fast determination of base metals in geochemical samples using the 5100 SVDV ICP-OES Application note Geochemistry, metals, mining Authors John Cauduro Agilent Technologies, Mulgrave, Australia Introduction
More informationAnalytical Ultracentrifugation in the Study of Protein Selfassociation and Heterogeneous Protein-Protein Interactions:
Analytical Ultracentrifugation in the Study of Protein Selfassociation and Heterogeneous Protein-Protein Interactions: Protocols for Velocity and Equilibrium Sedimentation Andrea Balbo and Peter Schuck
More informationThe Plus for your lab!
BioPhotometer plus NEW! analysis system The Plus for your lab! The easy, rapid and reliable BioPhotometer plus analysis system See it clearly! UVette Precision in plastics The patented* 1 UVette is the
More informationAnalytical Ultracentrifugation. by: Andrew Rouff and Andrew Gioe
Analytical Ultracentrifugation by: Andrew Rouff and Andrew Gioe Partial Specific Volume (v) Partial Specific Volume is defined as the specific volume of the solute, which is related to volume increase
More informationMeasuring S using an analytical ultracentrifuge. Moving boundary
Measuring S using an analytical ultracentrifuge Moving boundary [C] t = 0 t 1 t 2 0 top r bottom 1 dr b r b (t) r b ω 2 = S ln = ω 2 S (t-t dt r b (t o ) o ) r b = boundary position velocity = dr b dt
More informationThe power of multi-detector SEC in the analysis of antibodies
The power of multi-detector SEC in the analysis of antibodies How OMNISEC can measure antibody absolute MW independent of size and structural changes MOLECULAR SIZE MOLECULAR STRUCTURE MOLECULAR WEIGHT
More informationMake or Buy? The Economics of Gold Nanoparticle Manufacturing for Lateral Flow Assays
TECHNICAL RESOURCE Lateral Flow Immunoassays Make or Buy? The Economics of Gold Nanoparticle Manufacturing for Lateral Flow Assays Introduction Price is an important factor in the commercial success of
More informationcell, in which boundaries are formed under the stabilizing influence of
VOL. 38, 1952 CHEMISTRY: PICKELS ET AL. 943 AN ULTRACENTRIFUGE CELL FOR PRODUCING BOUNDARIES SYNTHETICALL Y B Y A LA YERING TECHNIQUE By E. G. PICKELS, W. F. HARRINGTON AND H. K. SCHACHMAN SPECIALIZED
More informationFar UV Performance of the LAMBDA 850/950 UV/Vis and UV/Vis/NIR Research Spectrophotometers
Far UV Performance of the LAMBDA 850/950 UV/Vis and UV/Vis/NIR Research Spectrophotometers UV/VIS AND UV/VIS/NIR SPECTROSCOPY A P P L I C A T I O N N O T E Summary The far UV region (generally covering
More informationMeet Our Uncle: 12 Stability Applications on One Platform
Tech Note Meet Our Uncle: 12 Stability Applications on One Platform Uncle is an all-in-one stability platform that enables twelve different applications with one instrument. Fluorescence, static light
More informationAnalytical Ultracentrifugation of Inorganic Colloids
Analytical Ultracentrifugation of Inorganic Colloids Sedimentation Velocity of Interacting and Non-Interacting Particles K.L. Planken Analytical Ultracentrifugation of Inorganic Colloids Sedimentation
More informationDetermination of Molecular Parameters by Fitting Sedimentation Data to Finite-Element Solutions of the Lamm Equation
444 Biophysical Journal Volume 74 January 1998 444 454 Determination of Molecular Parameters by Fitting Sedimentation Data to Finite-Element Solutions of the Lamm Equation Borries Demeler* and Hashim Saber
More informationINTRODUCTION TO ANALYTICAL ULTRACENTRIFUGATION
INTRODUCTION TO ANALYTICAL ULTRACENTRIFUGATION INTRODUCTION TO ANALYTICAL ULTRACENTRIFUGATION GREG RALSTON DEPARTMENT OF BIOCHEMISTRY THE UNIVERSITY OF SYDNEY SYDNEY, AUSTRALIA ii iii CONTENTS About the
More informationCONFOCHECK. Innovation with Integrity. Infrared Protein Analysis FT-IR
CONFOCHECK Infrared Protein Analysis Innovation with Integrity FT-IR CONFOCHECK: FT-IR System for Protein Analytics FT-IR Protein Analysis Infrared spectroscopy measures molecular vibrations due to the
More informationThe solution for all of your
The solution for all of your nanoparticle sizing and zeta potential needs. DelsaNano Series Blood Banking Capillary Electrophoresis Cell Analysis Centrifugation Genomics Lab Automation Lab Tools Particle
More informationUnderstanding the colloidal stability of protein therapeutics using dynamic light scattering
Understanding the colloidal stability of protein therapeutics using dynamic light scattering A Malvern Instruments' Bioscience Development Initiative Executive summary The combination of dynamic light
More informationMALVERN ADVANCED GPC/SEC DETECTORS SETTING THE STANDARD MOLECULAR WEIGHT MOLECULAR SIZE MOLECULAR STRUCTURE
MOLECULAR WEIGHT MOLECULAR SIZE MOLECULAR STRUCTURE MALVERN ADVANCED GPC/SEC DETECTORS SETTING THE STANDARD Setting the Standard WHAT CAN ADVANCED GPC/SEC DETECTION OFFER YOU? A modern research environment
More informationSelf-Assembly of Peptide Porphyrin Complexes: Towards the Development of Smart Biomaterials
Self-Assembly of Peptide Porphyrin Complexes: Towards the Development of Smart Biomaterials Brian C. Kovaric, Bashkim Kokona, Alexander D. Schwab, Margaret A. Twomey, Julio C. de Paula, and Robert Fairman
More informationDesign Considerations for a Variable Angle Absolute Reflectance Accessory For the LAMBDA 950/850/650 UV/Vis/NIR and UV/Vis Spectrophotometers
Design Considerations for a Variable Angle Absolute Reflectance Accessory For the LAMBDA 950/850/650 UV/Vis/NIR and UV/Vis Spectrophotometers UV/VIS AND UV/VIS/NIR SPECTROSCOPY A P P L I C A T I O N N
More informationSupplementary Figure 2. Negative stain EM reconstructions. 4
Supplementary Information for: EM Structure of human APC/C Cdh1 -EMI1 reveals multimodal mechanism E3 ligase shutdown Item Page Supplementary Figure 1. Analytical Ultracentrifugation of EMI1 DLZT. 2 Supplementary
More informationHow to use GPC/SEC for compositional analysis
How to use GPC/SEC for compositional analysis Determining the relative concentration of two components in a polymer sample MOLECULAR SIZE MOLECULAR STRUCTURE MOLECULAR WEIGHT Introduction Over the last
More informationIRDye 800CW Protein Labeling Kit Low MW
IRDye 800CW Protein Labeling Kit Low MW Developed for: Aerius, and Odyssey Family of Imagers Please refer to your manual to confirm that this protocol is appropriate for the applications compatible with
More informationPhysisorption of Antibodies using BioReady Bare Nanoparticles
TECHNICAL RESOURCE Lateral Flow Immunoassays Physisorption of Antibodies using BioReady Bare Nanoparticles Introduction For more than 20 years, lateral flow immunoassay diagnostic tests have provided a
More informationDiffusion-deconvoluted sedimentation coefficient distributions for the analysis of interacting and non-interacting protein mixtures
1 Chapter for Modern Analytical Ultracentrifugation: Techniques and Methods (D.J. Scott, S.E. Harding, A.J. Rowe, Editors) The Royal Society of Chemistry, Cambridge (in press) Diffusion-deconvoluted sedimentation
More informationImproved Automated Sample Preparation for the Analysis of 25-OH-Vitamin D3 by LC/MS/MS
Improved Sample Preparation for the Analysis of 25-OH-Vitamin D3 by LC/MS/MS Abstract In this work, we demonstrate an improved automated sample preparation for liquid chromatography-tandem mass spectrometry
More informationA B C SHORT COLUMN SEDIMENTATION EQUILIBRIUM ANALYSIS FOR RAPID CHARACTERIZATION OF MACROMOLECULES IN SOLUTION. Inches.
APPLICATION WHITE NOTE PAPER Thomas M. Laue University of New Hampshire Department of Biochemistry Durham, NH 03824 Introduction SHORT COLUMN SEDIMENTATION EQUILIBRIUM ANALYSIS FOR RAPID CHARACTERIZATION
More informationPREPRINT (this article appeared in Analytical Biochemistry 279, , 2000)
PREPRINT (this article appeared in Analytical Biochemistry 79, 151-163, 000) A Method for Directly Fitting the Time Derivative of Sedimentation Velocity Data and an Alternative Algorithm for Calculating
More informationAchieve confident synthesis control with the Thermo Scientific ISQ EC single quadrupole mass spectrometer
APPLICATION NOTE 72385 Achieve confident synthesis control with the Thermo Scientific ISQ EC single quadrupole mass spectrometer Authors Stephan Meding, Katherine Lovejoy, Martin Ruehl Thermo Fisher Scientific,
More informationThe Coulter Principle for Outlier Detection in Highly Concentrated Solutions
The Coulter Principle for Outlier Detection in Highly Concentrated Solutions Particle Characterization Application Note Abstract This application note will benefit chemical and material manufacturers concerned
More informationMeasurements of protein electrophoretic mobility using the Zetasizer Nano ZSP
Measurements of protein electrophoretic mobility using the Zetasizer Nano ZSP Introduction Zetasizer Nano is the market leader in dynamic and electrophoretic light scattering technology for measurements
More informationMaking Accurate Field Spectral Reflectance Measurements By Dr. Alexander F. H. Goetz, Co-founder ASD Inc., Boulder, Colorado, 80301, USA October 2012
Making Accurate Field Spectral Reflectance Measurements By Dr. Alexander F. H. Goetz, Co-founder ASD Inc., Boulder, Colorado, 80301, USA October 2012 Introduction Accurate field spectral reflectance measurements
More informationNon-Interfering Protein Assay Kit Cat. No
Visit our interactive pathways at /pathways User Protocol 488250 Rev. 5 January 2006 RFH Page 1 of 1 Non-Interfering Protein Assay Kit Cat. No. 488250 Note that this user protocol is not lot-specific and
More informationUV-Vis optical fiber assisted spectroscopy in thin films and solutions
UV-Vis optical fiber assisted spectroscopy in thin films and solutions Description UV-Visible absorption and transmission spectra provide fundamental information for all experiments related to the attenuation
More informationCOURSE MATERIAL: Unit 3 (Part 1) Polymer Science LT8501 (Click the link Detail to download)
COURSE MATERIAL: Unit 3 (Part 1) Polymer Science LT8501 (Click the link Detail to download) Dr. Debasis Samanta Senior Scientist & AcSIR Assistant Professor Polymer Science & Technology Department., CSIR-CLRI,
More informationInformation Technology Solutions Neptune Datasheet
Information Technology Solutions Neptune Datasheet Suspended Solids Analyzer Neptune is the first purpose built in-situ analyzer for measuring the suspended solids of slurries. Specifically developed for
More informationCyFlow Ploidy Analyser & CyFlow Space High-resolution DNA analysis
CyFlow Ploidy Analyser & High-resolution DNA analysis For agroscience breeding aquaculture CyFlow Ploidy Analyser www.sysmex-flowcytometry.com Dedicated solutions for ploidy analysis and determining genome
More informationProtein Quantitation using a UV-visible Spectrophotometer
UV-0003 UV-visible Spectrophotometer Introduction Generally, protein quantitation can be made using a simple UV-Visible spectrophotometer. The V-630 Bio (Figure 1) is a UV- Visible spectrophotometer designed
More informationOne-stop stability. T m & T agg. T m with SYPRO (DSF) Isothermal stability. Thermal recovery. Sizing & polydispersity. Sizing with thermal ramp B 22
One-stop stability Cracking stability using a pile of one-trick, protein-hungry tools is a ton of work. UNcle combines 3 different measurement modes fluorescence, SLS and DLS. So you can crank out all
More informationIntroduction to Fourier Transform Infrared Spectroscopy
Introduction to Fourier Transform Infrared Spectroscopy Introduction What is FTIR? FTIR stands for Fourier transform infrared, the preferred method of infrared spectroscopy. In infrared spectroscopy, IR
More informationDetection of Protein Aggregation by Enzyme Immunoassay
Detection of Protein Aggregation by Enzyme Immunoassay Protein Aggregation Detection (PAD) Technology Microsens Biotechnologies www.microsens.co.uk Protein Aggregation Detection (PAD) Technology 1. The
More informationSupporting Information
Supporting Information Characterizing the Effect of Salt and Surfactant Concentration on the Counter-ion Atmosphere around Surfactant Stabilized SWCNTs using Analytical Ultracentrifugation Stephanie Lam
More informationTL6 Ultra Micro-volume Spectrophotometer
TL6 Ultra Micro-volume Spectrophotometer TL6 is the latest Ultra Micro-volume Spectrophotometer of PERSEE ANALYTICS. It is a small portable instrument for fast analysis of nucleic acid and protein concentrations
More informationnext generation Welcome to the Dispersion options Smarter particle sizing Your sample, expertly processed and prepared Particle size
Particle size Welcome to the next generation Your sample, expertly processed and prepared Dispersion options 3000 detailed specification sheets from Getting your sample preparation right The US Pharmacopeia
More informationICP-OES Application Note Number 35
ICP-OES Application Note Number 35 Rapid measurement of major, minor and trace levels in soils using the Varian 730-ES Vincent Calderon Varian, Inc. Introduction As part of the global strategy for sustainable
More informationUse of DLS/Raman to study the thermal unfolding process of lysozyme
Use of DLS/Raman to study the thermal unfolding process of lysozyme A Malvern Instruments' Bioscience Development Initiative Executive summary The combination of dynamic light scattering (DLS) and Raman
More informationApplication Note Antibody-SOMAmer Sandwich Assay
Application Note Antibody-SOMAmer Sandwich Assay Introduction SOMAmer reagents (Slow Off-rate Modified Aptamers) are DNA-based high affinity (average Kd < 1 nm) protein binding reagents with proprietary
More informationSEC MALLs and AUC. 2. Conformation and flexibility Viscometry,
1. Molecular weight distribution analysis SEC MALLs and AUC 2. Conformation and flexibility Viscometry, AUC, Light scattering Lecture 5 Analytical Ultracentrifugation II: interactions Steve Harding Free
More informationBECKMAN COULTER QbD1200 Total Organic Carbon Analyzer Critical Measurements Made Simple
BECKMAN COULTER QbD1200 Total Organic Carbon Analyzer Critical Measurements Made Simple Reliable TOC measurements Stop throwing away your first rep. QbD1200 has virtually eliminated sample-to-sample carryover
More informationAnalytical methods to characterize aggregates and subvisible particles
Analytical methods to characterize aggregates and subvisible particles Andrea Hawe 9 th P4EU Meeting 30 th November 2015 * MPI * Martinsried 1 Background of company privately held, independent service
More informationIntroduction to Dynamic Light Scattering for Particle Size Determination
www.horiba.com/us/particle Jeffrey Bodycomb, Ph.D. Introduction to Dynamic Light Scattering for Particle Size Determination 2016 HORIBA, Ltd. All rights reserved. 1 Sizing Techniques 0.001 0.01 0.1 1 10
More informationAnalytical Ultracentrifugation
Analytical Ultracentrifugation Stephen McLaughlin Biophysics MRC Laboratory of Molecular Biology Macromolecular Dynamics D Folding N Binding K N-D K D k D agg Aggregation Crystallisation 1 Macromolecular
More informationAnalysis of domestic sludge using the Agilent 4200 MP-AES
Analysis of domestic sludge using the Agilent 4200 MP-AES Application note Environmental Authors Neli Drvodelic Agilent Technologies, Melbourne, Australia Introduction Managing the treatment and disposal
More informationISoTherMal TITraTIon Calorimetry
ISoTherMal TITraTIon Calorimetry With the Nano ITC, heat effects as small as 1 nanojoules are detectable using one nanomole or less of biopolymer. The Nano ITC uses a solid-state thermoelectric heating
More informationIRDye 680RD Protein Labeling Kit Low MW
IRDye 680RD Protein Labeling Kit Low MW Developed for: Odyssey Family of Imagers Please refer to your manual to confirm that this protocol is appropriate for the applications compatible with your Odyssey
More informationMeeting the Challenges of Soil Analysis with the Avio 200 ICP-OES
APPLICATION NOTE ICP-Optical Emission Spectroscopy Author: Nick Spivey PerkinElmer, Inc. Shelton, CT Meeting the Challenges of Soil Analysis with the Avio 200 ICP-OES Introduction Micronutrients contained
More informationModern analytical ultracentrifugation in protein science: A tutorial review
REVIEW Modern analytical ultracentrifugation in protein science: A tutorial review JACOB LEBOWITZ, MARC S. LEWIS, AND PETER SCHUCK Molecular Interactions Resource, Division of Bioengineering and Physical
More informationEppendorf UVette. Instructions for use
Instructions for use Instructions Eppendorf see Fig. Tab. p. p. UVette for use Copyright 2014 Eppendorf AG, Hamburg, Germany. All rights reserved, including graphics and images. No part of this publication
More informationNano Spectrophotometer LNS-A10
Nano Spectrophotometer LNS-A10 Nano spectrophotometer with full spectrum light covers wide wavelength range of 200 to 800nm. It requires only 0.5-2.0µl micro-volume sample for measurement which takes place
More informationThe Approach to Equilibrium in a Buoyant-Density Gradient
BIOPOLY MERS VOL. 11, 17651769 (1972) The Approach to Equilibrium in a BuoyantDensity Gradient CARL W. SCHMID and JOHN E. HEARST, Department of Chemistry, University of California Berkeley, California
More informationCenter for Cell Imaging Department of Cell Biology
Center for Cell Imaging Department of Cell Biology Contents Preparation of Colloidal Gold Conjugates Coupling the Protein A to the Gold Particles Purification of the protein A-gold. Storage Influence of
More informationQuantitative Determination of Proteins
Application Note UV-0003-E Quantitative Determination of Proteins Generally, the concentration of proteins is measured using UV-Vis spectrophotometers. The JASCO V-630 Bio (Figure 1) is a UV/Vis spectrophotometer
More informationGE Healthcare Life Sciences. Spectrophotometry. Handbook
GE Healthcare Life Sciences Spectrophotometry Handbook Contents Spectrophotometry basics 3 What is spectrophotometry? 3 Definition 3 Lambert s Law 4 Beer s Law 4 Nucleic acid applications 6 Direct UV measurement
More informationRamanStation 400: a Versatile Platform for SERS Analysis
FIELD APPLICATION REPORT Raman Spectroscopy Author: Dean H. Brown PerkinElmer, Inc. Shelton, CT USA RamanStation 400 RamanStation 400: a Versatile Platform for SERS Analysis Introduction Surface Enhanced
More informationONYX -MCE MULTI-CHANNEL OPTICAL FIBER PYROMETERS WITH ACTIVE EMISSIVITY COMPENSATION PRECISION TEMPERATURE MEASUREMENT FOR DEMANDING INDUSTRIAL
ONYX -MCE MULTI-CHANNEL OPTICAL FIBER PYROMETERS WITH ACTIVE EMISSIVITY COMPENSATION PRECISION TEMPERATURE MEASUREMENT FOR DEMANDING INDUSTRIAL APPLICATIONS Accurate, repeatable, and reliable temperature
More informationNow also in Eppendorf LoBind. Conical Competence. Eppendorf Conical Tubes 15 ml and 50 ml
Now also in Eppendorf LoBind Conical Competence Eppendorf Conical Tubes 15 ml and 50 ml 2 Eppendorf Conical Tubes The Eppendorf Tubes Best Performance for Your Samples The Eppendorf Conical Tubes 15 ml
More informationBiochrom Libra. UV-Visible Spectrophotometers. High performance instruments with added application value for academia, research and industry
New Double Beam Spectrophotometers Biochrom Libra UV-Visible Spectrophotometers High performance instruments with added application value for academia, research and industry Biochrom Libra Real Choice
More informationC101-E112. BioSpec-nano. Shimadzu Spectrophotometer for Life Science
C101-E112 BioSpec-nano Shimadzu Spectrophotometer for Life Science Power of small. BioSpec-nano BioSpec-nano Shimadzu Spectrophotometer for Life Science Quick and Simple Nucleic Acid Quantitation Drop-and-Click
More informationAutomating Gradient Method Development in Flash Chromatography For Greater Productivity and Minimizing Solvent Use
Automating Gradient in Flash For Greater Productivity and Minimizing Solvent Use Melissa Wilcox, Rakesh Bose, Scott Anderson, Kathy Lawrence Grace Davison Discovery Sciences 251 Waukegan Rd. Deerfield
More informationEVOLUTE WCX Columns for Solid Phase Extraction of Quaternary Amines and Strongly Basic Compounds from Aqueous Samples
TN143 EVOLUTE WCX Columns for Solid Phase Extraction of Quaternary Amines and Strongly Basic Compounds from Aqueous Samples This Chemistry Data Sheet provides guidelines for the extraction of quaternary
More informationLecture 5. More on UV-visible Spectrophotometry: Beer s Law and Measuring Protein Concentration
Biological Chemistry Laboratory Biology 3515/Chemistry 3515 Spring 2018 Lecture 5 More on UV-visible Spectrophotometry: Beer s Law and Measuring Protein Concentration 23 January 2018 c David P. Goldenberg
More informationCharacterization of bovine serum albumin/ chlorogenic acid solution mixtures by analytical ultracentrifugation
Progr Colloid Polym Sci (2004) 127: 83 88 DOI 10.1007/b98017 Ó Springer-Verlag 2004 A. Seifert H. M. Rawel S. E. Harding J. Kroll Characterization of bovine serum albumin/ chlorogenic acid solution mixtures
More informationBy Mohsin Al-Saleh Clinical Biochemistry Department (SQUH)
Laboratory Automation By Mohsin Al-Saleh Clinical Biochemistry Department (SQUH) Objectives: Define automation and lab automation Advantages of laboratory automation History of laboratory automation Fluidic
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION Solvent-free protein function - reversible dioxygen binding in liquid myoglobin Adam W. Perriman 1, Alex P. S. Brogan 1, Helmut Cölfen 2, Nikos Tsoureas 3, Gareth R. Owen 3 Mann
More informationFor the rapid, sensitive and accurate measurement of Urea levels in various samples.
ab83362 Urea Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Urea levels in various samples. This product is for research use only and is not intended for diagnostic
More informationUltra- centrifugation (Gradient centrifugation), Viscometry, UV-VIS absorption spectroscopy, Mass spectrophotometery.
Ultra- centrifugation (Gradient centrifugation), Viscometry, UV-VIS absorption spectroscopy, Mass spectrophotometery Mitesh Shrestha Sedimentation Sedimentation describes the motion of molecules in solutions
More informationProtein-Ligand Interactions: hydrodynamics and calorimetry
Protein-Ligand Interactions: hydrodynamics and calorimetry Approach Stephen E. Harding Babur Z. Chowdhry OXFORD UNIVERSITY PRESS , New York Oxford University Press, 2001 978-0-19-963746-1 List of protocols
More informationspecified quantity of a solvent at a given temperature. To deconvolute the value from the
S.1 Calculations of Dilution Enthalpy and Enthalpic Interaction Coefficients. When a solute is dissolved in a solvent a solution is formed. During dissolution of a solute in any solvent, heat is either
More informationSize-Distribution Analysis of Macromolecules by Sedimentation Velocity Ultracentrifugation and Lamm Equation Modeling
1606 Biophysical Journal Volume 78 March 2000 1606 1619 Size-Distribution Analysis of Macromolecules by Sedimentation Velocity Ultracentrifugation and Lamm Equation Modeling Peter Schuck Molecular Interactions
More informationSEDIMENTATION VELOCITY ANALYTICAL ULTRACENTRIFUGATION
Analytical Biochemistry, in press ON THE ANALYSIS OF PROTEIN SELF-ASSOCIATION BY SEDIMENTATION VELOCITY ANALYTICAL ULTRACENTRIFUGATION Peter Schuck Protein Biophysics Resource, Division of Bioengineering
More informationImagent for fnirs and EROS measurements
TECHNICAL NOTE Imagent for fnirs and EROS measurements 1. Brain imaging using Infrared Photons Brain imaging techniques can be broadly classified in two groups. One group includes the techniques that have
More informationISA QATAR : 90 th Seminar
ANALYTICAL INSTRUMENTATION & MAINTENANCE SYSTEMS (AIMS) ISA QATAR : 90 th Seminar UTILIZATION OF LASERS IN THE FIELD OF GAS ANALYZERS Presenter: ZAHEER JUDDY Electromagnetic Spectrum Electromagnetic Spectrum
More informationFSA TM Multi-bit Digital Processors
Laser Diagnostics FSA TM Multi-bit Digital Processors Revolutionary, State-of-the- Art Digital Signal Processing for Velocity and Size TSI is the only instrument supplier that developed two powerful, digital
More informationSedimentation Velocity Analysis of Interacting Systems using c(s) Peter Schuck
Sedimentation Velocity Analysis of Interacting Systems using c(s) Peter Schuck simulated sedimentation of rapidly self-association systems monomer/dimer monomer/trimer 5 concentration (Kd) 4 2 concentration
More informationSmart Sensing Embedded Spectroscopy Platform Botlek studiegroep 06-april-2017
Smart Sensing Embedded Spectroscopy Platform Botlek studiegroep 06-april-2017 W. Karremans Personal introduction Background: Process Analysis DSM AKZO Nobel Chemicals Aspenpharma 2016: Sales Engineer Elscolab
More informationSpectrophotometer for Life Science. BioSpec-nano C101-E112D
Spectrophotometer for Life Science BioSpec-nano C11-E112D BioSpec-nano Spectrophotometer for Life Science Drop-and-Start Analysis Automatic Optical Pathlength Setting & Automatic Wiping Low Carryover Achieved
More informationProduct Guide. Thermo Scientific Cellomics HCS Solution
Product Guide Thermo Scientific Cellomics HCS Solution Thermo Scientific Cellomics HCS Solution Your Complete HCS Solution The search for new therapies for human diseases is increasingly driven by the
More informationMeasuring Lysozyme Monomer at 0.1 mg/ml Concentration. Equipment used : Sample Preparation and Measurement :
Application Report #001 Measuring Lysozyme Monomer at 0.1 mg/ml Concentration Equipment used : ALV-NIBS / HPPS High Sensitivity Version, Lysozyme (MERCK), 0.1 molar Sodium-Acetate buffer (ph 4.25), syringe
More informationIntroduction to Dynamic Light Scattering with Applications. Onofrio Annunziata Department of Chemistry Texas Christian University Fort Worth, TX, USA
Introduction to Dynamic Light Scattering with Applications Onofrio Annunziata Department of Chemistry Texas Christian University Fort Worth, TX, USA Outline Introduction to dynamic light scattering Particle
More informationAutomated purification of high quality genomic DNA
APPLICATION NOTE No. AA267 I October 2012 Automated purification of high quality genomic DNA from various tissues using the Eppendorf MagSep Tissue gdna Kit on the Eppendorf epmotion M5073 Ulrich Wilkening,
More informationApplications Note 202 October 2008
Applications Note 202 October 2008 Technical Report Eppendorf Polypropylene Microplates Fast and secure identification of samples Natascha Weiß 1, Wolf Wente 2, Sophie Freitag 2, Daniel Wehrhahn 1 1 Eppendorf
More information