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1 Supporting Information Ultrasensitive Label-Free Resonance Rayleigh Scattering Aptasensor for Hg 2+ Using Hg 2+ -Triggered Exonuclease III-Assisted Target Recycling and Growth of G-Wires for Signal Amplification Wang Ren, a, b Ying Zhang, a, b Hong Guo Chen, a Zhong Feng Gao, a Nian Bing Li a, * and Hong Qun Luo a, * a Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing , People s Republic of China b College of Chemistry and Pharmaceutical Engineering, Sichuan Provincial Academician (Expert) Workstation, Key Laboratory of Green Catalysis of Higher Education Institutes of Sichuan, Sichuan University of Science and Engineering, Zigong , People s Republic of China * Corresponding Author, luohq@swu.edu.cn (H. Luo), linb@swu.edu.cn (N. Li) S-1
2 Supporting Tables: Tables S1 to S2 Supporting Figures: Figures S1 to S6 Supporting Information (SI) Contents Table S1 Sequence of Oligomers Table S2 Comparison of the Proposed Approach with Other Reported Methods for the Detection of Hg Figure S1 Atomic Force Microscopy... 5 Figure S2 Optimum Concentrations of Exo-Ш Catalysis 6 Figure S3 Optimum Temperature for the Exo-Ш Catalysis...7 Figure S4 Optimum Time of Exo-Ш Catalysis.8 Figure S5 Optimum Concentration of Mg 2+ for the G-Wire Formation... 9 Figure S6 Optimum Incubation Time of Mg References S-2
3 1. Table S1 Table S1 Sequence of Oligomers Oligomer Sequence (from 5 to 3 ) H 1 (c-myc) H 2 (c-myc) H 3 (c-myc) H 4 (c-myc) H 5 (c-myc) H 6 (PS2.M) H 6 (i-motif) H 7 (normally) TTTTAGGGTGGGGAGGGTGGGGCCCCACCCTTTTT CTTTAGGGTGGGGAGGGTGGGGCCCCACCCTTTAG CTTTAGGGTGGGGAGGGTGGGGCCCCACCCTTAAG TTTAGGGTGGGGAGGGTGGGGCCCCACCCTTTT CTTTAGGGTGGGGAGGGTGGGGCCCCACCCTTTTG CTTTGTGGGTAGGGCGGGTTGGCCTACCCACTTTG CTTTCCCTAACCCTAACCCTAACCCGGGTTAGGGTTTG CTTTCGAACAGC AA GCAGACTG TTGCTGTTCGTTTG c-myc: parallel-stranded G-quadruplex topology; PS2.M: antiparallel-stranded G-quadruplex topology; i-motif: i-motif topology. S-3
4 2. Table S2 Comparison of the Proposed Approach with Other Reported Methods for the Detection of Hg 2+ SERS: Surface-enhanced Raman scattering; PDDA: Poly (diallyldi-methylammonium chloride); AuNPs: gold nanoparticles; ABTS 2- : 2,2 -azino-bis(3-ethylbenzothiazo-line-6-sulfonate) disodium salt. Method Label or signal reagent Linear range (nm) LOD (pm) Ref. Electrochemistry AuNPs and methyl blue S1 Electrochemistry Ru(NH 3 ) 6 Cl S2 Electrochemistry Ferrocene S3 Electrochemistry Methylene blue S4 Electrochemistry Methylene blue S5 Luminescence Iridium(III) complex S6 Fluorescence Phosphorothioate RNA S7 SERS Cyanine S8 Fluorescence Mn:CdS/ZnS and AuNPs S9 Fluorescence Polymer Carbon Nanoribbons S10 Fluorescence Ag nanoclusters S11 Fluorescence Thioflavin T S12 Fluorescence Ag nanoclusters S13 Colorimetry PDDA S14 Colorimetry AuNPs S15 Colorimetry Ag nanowire S16 Colorimetry AuNPs S17 Colorimetry AuNPs S18 Colorimetry ABTS S19 RRS Free This work S-4
5 3. Atomic Force Microscopy Figure S1. AFM images of the resultant products in buffer solution containing (A) 10.0 µm H U Exo-Ш; (B) 10.0 µm H U Exo-Ш µm Hg 2+. S-5
6 4. Optimum Concentrations of Exo-Ш Catalysis 2500 I RRS C Exo-III / U Figure S2 Effect of different concentrations of Exo-III on the RRS intensity responding to the Exo-Ш catalysis process. (The concentration of DNA and Mg 2+ were 0.2 µm and 0.15 M, respectively. Reaction temperature and time were 30 C and 30 min, respectively; Incubation time: 2 h). The error bars represent the standard deviation of three parallel measurements (the same below). S-6
7 5. Optimum Temperature for the Exo-Ш Catalysis I RRS Without Hg nm Hg T / o C Figure S3 Effect of different reaction temperatures on the RRS intensity responding to the Exo Ш catalysis process. (The concentration of DNA and Mg 2+ were 0.2 µm, 0.15 M, respectively. Reaction time was 30 min; Incubation time: 2 h; Exo-III: 25 U) S-7
8 6. Optimum Time of Exo-Ш Catalysis I RRS t / min Figure S4 Effect of different reaction time on the RRS intensity responding to the Exo-Ш catalysis process. (The concentration of DNA and Mg 2+ were 0.2 µm, 0.15 M, respectively. Reaction temperature was 35 C; Incubation time: 2 h; Exo-III: 25 U) S-8
9 7. Optimum Concentration of Mg 2+ for the G-wire Formation 4000 I RRS Without Hg nm Hg C Mg 2+ / M Figure S5 Effect of different concentrations of Mg 2+ on the RRS intensity responding to G-wire formation process in the presence of Hg 2+ (upper line) and in the absence of Hg 2+ (under line) under the optimal conditions. (The concentration of DNA was 0.2 µm. Reaction temperature and time were 35 C and 50 min, respectively; Incubation time: 2 h; Exo-III: 25 U) S-9
10 8. Optimum Incubation Time of Mg I RRS t / min Figure S6 Effect of incubation time on the RRS intensity responding to 10.0 nm Hg 2+ in the presence of 0.2 M Mg 2+. (The concentration of DNA was 0.2 µm. Reaction temperature and time were 35 C and 50 min, respectively; Exo-III: 25 U) S-10
11 References S1) Zhang, Y.; Zeng, G. M.; Tang, L.; Chen, J.; Zhu, Y.; He, X. X.; He, Y. Anal. Chem. 2015, 87, S2) Bao, T.; Wen, W.; Zhang, X.; Xia, Q.; Wang, S. Biosens. Bioelectron. 2015, 70, S3) Zhuang, J.; Fu, L.; Tang D.; Xu, M.; Chen, G.; Yang, H. Biosens. Bioelectron. 2013, 39, S4) Xuan, F.; Luo, X.; Hsing, I. M. Anal. Chem. 2013, 85, S5) Tortolini, C.; Bollella, P.; Antonelli, M. L.; Antiochia, R.; Mazzei, F.; Favero, G. Biosens. Bioelectron. 2015, 67, S6) Ru, J.; Chen, X.; Guan, L.; Tang, X.; Wang, C.; Meng, Y.; Zhang, G.; Liu, W. Anal. Chem. 2015, 87, S7) Huang, P. J.; Wang, F.; Liu, J. Anal. Chem. 2015, 87, S8) Sun, B.; Jiang, X.; Wang, H.; Song, B.; Zhu, Y.; Wang, H.; Su, Y.; He, Y. Anal. Chem. 2015, 87, S9) Huang, D.; Niu, C.; Wang, X.; Lv, X.; Zeng, G.. Anal. Chem. 2013, 85, S10) Wang, Z.; Ding, S. Anal. Chem. 2014, 86, S11) Deng, L.; Zhou, Z.; Li, J.; Li, T.; Dong, S. Chem. Commun. 2011, 47, S12) Ge, J.; Li, X.; Jiang, J.; Yu, R. Talanta 2014, 122, S13) Wang, G.; Xu, G.; Zhu, Y.; Zhang, X. Chem. Commun. 2014, 50, S14) Zhu, Y.; Cai, Y.; Zhu, Y.; Zheng, L.; Ding, J.; Quan, Y.; Wang, L.; Qi, B. Biosens. Bioelectron.2015, 69, S15) Gao, Y.; Li,X.; Li,Y.; Li, T.; Zhao,Y.; Wu, A. Chem. Commun. 2014, 50, S16) Tang, S.; Tong, P.; Wang, M.; Chen, J.; Li, G.; Zhang, L. Chem. Commun., 2015, 51, S17) Sener, G.; Uzun, L.; Denizli, A. Anal. Chem. 2014, 86, S18) Chen, J.; Zhou, S.; Wen, J. Anal. Chem. 2014, 86, S19) Ren, W.; Zhang, Y.; Huang, W. T.; Li, N. B.; Luo, H. Q. Biosens. Bioelectron. 2015, 68, S-11
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Supporting Information Surfactant-Free Assembly of Mesoporous Carbon Hollow Spheres with Large Tunable Pore Sizes Hongwei Zhang, Owen Noonan, Xiaodan Huang, Yannan Yang, Chun Xu, Liang Zhou, and Chengzhong
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Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2015 Supporting Information Synthesis and electrochemical properties of spherical and hollow-structured
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Electronic Supplementary Material (ESI) for Journal of Materials Chemistry A. This journal is The Royal Society of Chemistry 2017 Electronic Supplementary Information Cation exchange MOF-derived nitrogen-doped
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Supporting Information A Rational Solid-state Synthesis of Supported Au-Ni Bimetallic Nanoparticles with Enhanced Activity for Gas-phase Selective Oxidation of Alcohols Wuzhong Yi, a Wentao Yuan, b Ye
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