Acoustic Wave Sensors
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1 Acoustic Wave Sensors Professor Glen McHale School of Science & Technology January 2008
2 Overview 1. The Lab 2. Basics of Acoustic Waves Sensing principles Acoustic wave modes and devices 3. Layer-Guided Acoustic Waves Love waves & acoustic plate modes Layer-guided devices: Theory of operating points and sensitivity Layer-guided devices: Experimental confirmation 4. Sensor Research Examples Atmospheric science: Particulate detection Biological sciences: Peptide binding, steroids, sperm motility Chemistry/Chemical engineering: Green solvents Basic devices: Sectional guiding layers 5. Overview of Wetting 02 September
3 The Laboratory 02 September
4 The Laboratory Themes & Expertise Acoustic waves sensors Quartz crystals and surface acoustic waves Wetting of surfaces Topography+chemistry, superhydrophobicity Materials scientists Physicists Multidisciplinary People 2 x Academics (Physicists, >2 incl. others) 3 x PhD Students (+ others joint) Physicists by first degree 5 x Research fellows Electrochemist/Physical chemist Applied physicist/acoustic waves Inorganic/protein chemist Materials synthesis (sol-gel) Engineer/Microfluidics Science Acoustic wave sensors Theory of layer guided devices Applications of QCM and SAWs Wetting & topography New superhydrophobic surfaces, superspreading Lquid marbles, electrowetting, hydrophobic soil Slip and slip boundary conditions Facilities Device and surface fabrication Lithography (<2 µm), metal deposition Inorganic/materials lab Surface characterisation SEM/TEM/Confocal microscopy Contact/non-contact profilometry Instrumentation & measurement RF Network analyzers, QCMs, laser cutting Krüss DSA, high speed camera, kv supplies, 02 September
5 Basics of Acoustic Waves 02 September
6 QCM Sensing Principles Thickness Shear Mode Vibration Quartz crystal microbalance - sharp resonance Frequency given by quartz thickness, w v s =fλ f=2v s /w w Mass Loading or Immersion Frequency reduces due to mass Resonance broadens due to polymer/liquid Sauerbrey equation f f 2 m/a Kanazawa & Gordon f (ηρ) f 3/2 Sensitivity to mass or viscosity-density product increases with frequency 02 September
7 Liquids and Penetration Depth Shear Mode Vibration Entrains liquid Liquid oscillation decays Penetration depth δ=(η/πfρ) 1/2 Liquid Sensing Sense liquid mass (via viscosity-density product) within penetration depth QCM SAW For water 5 MHz δ ~ 250 nm 500 MHz δ ~ 25 nm Penetration depth/sensing zone decreases with frequency 02 September
8 Acoustic Waves Acoustic Waves QCM versus SAW QCM frequency determined by thickness SAW frequency determined by fingers 02 September
9 Acoustic Wave Modes Delay Lines Resonators Mirror L Mirror Mirror L Mirror L R L R L T L T 02 September
10 SAW Transducer Design IDT Configurations single-single single-double double-split six-finger split-joined λ IDT λ IDT λ IDT λ IDT λ IDT Different spatial and electrical periods Harmonic operation: double-double gives higher frequency for same fabrication limits possible multi-frequency operation along same path Reflectivity: double-split and split-joined avoids reflections Possible Design Considerations Aperture: matching and beam/beam spreading Number of fingers: bandwidth End effects and guard electrodes Reflecting structures: distributed reflection Bidirectional and unidirectional transducers Wireless operation 02 September
11 Acoustic Waves - Comparisons Thickness Shear Mode Quartz crystal microbalance (QCM) Surface Acoustic Waves (SAWs) Rayleigh waves, Love waves, Surface transverse waves (STWs), Lamb waves/flexural plate waves (FPWs) Acoustic Plate Modes (APMs) Shear horizontally polarised SAWs (SH-SAWs) Surface skimming bulk waves (SSBWs) Mode Rel. Sens. Complexity Robustness Gas/Liquid QCM Low Low/Xtal Med g+l SAW High Med/metal on Xtal High g Love High Med/film+metal+Xtal High g+l STW High Med/metal on Xtal High g+l Lamb High High/membrane Low g+l APM Med Med/metal on Xtal Med g+l 02 September
12 Layer-Guided Acoustic Waves 02 September
13 Love Waves versus SH-APMs Love Wave SH-APM Layer guided SH-SAW with v l < v s Surface localised wave Increased sensitivity QCM with propagation Substrate resonance Sensing via both faces Increased sensitivity versus isolation between sensing face and transduction 02 September 2009 McHale, et al, J. Appl. Phys., 2003, 93, ; MST, 2003, 14,
14 Layer-Guided SH-APMs Theoretical Dispersion Curve Love device with thinned substrate Love waves for v<v s Shear mode in substrate-to-shear mode in layer transition P hase S pee d, v m s st mode 2 nd mode 3 rd mode APM s v=v s Love Waves SH-APMs for v>v s 1000 v=v l Mass/liquid sensitivity slope dêl l 2000 Love Wave Sensitivity 400 SH-APM Sensitivity S ensitivity,»sm» m 2 k g S ensitivity,»s m» m 2 k g dêl l dêl l 02 September 2009 McHale, et al, J. Appl. Phys.., 2002, 91, ;
15 Generalized Love Waves Operating Point P hase S pee d, v m s Operating point 1 st Mode 2 nd mode 3 rd mode dêl l (guiding layer thickness) APM s v=v s Love Waves v=v l Love wave = Shear mode in substrate-to-shear mode in layer transition Plate modes = Switch in order of resonance induced by layer Increased mass/liquid sensitivity related to slope of dispersion curve 02 September
16 Phase Speed Mass Sensitivity S m = 1 v lim m 0 m v o fo ρ v m is mass per unit area being sensed, z=df/v l is the normalized thickness "Rigid" mass Mass sensitivity is slope of dispersion curve l l d log dz e v z 0 Love Waves Layer-Guided SH-APMs S ensitivity,»sm» m 2 k g st mode 2 nd mode 3 rd mode S ensitivity,»s m» m 2 k g dêl l dêl l 02 September
17 02 September Polymer Waveguide on Polymer Substrate Complex velocity shift ( ) l l f o f o f z z e o l o f o v h h T h T dz v d v v v v v v o ρ π ωρ = 2 tan log Generalized Sauerbrey/Kanazawa & Gordon Complex slope factor from polymer waveguide ω m/a (ρηω) 1/2 ( ) ( ) 0 and tan 2 2 ωτ ωρ η h v v h j h h T h T f f o f o f o f tanx/x factor gives mass/liquid loading limits Sauerbrey/ solid limit Kanazawa & Gordon/liquid limit McHale, et al, J. Appl. Phys., 2003, 93, ; MST, 2003, 14,
18 Multiple Love Wave Modes Spectra Thick guiding layers Photoresist layers Quartz substrate (SSBW) Experimental Results Points = results for devices 110/330 and 309 MHz Lines = theory Insertion Loss/dB Later decrease Frequency/MHz Initial increase P hase Spee d dêl l 02 September 2009 Newton, et al, Euro. Phys. Lett., 2002, 58,
19 Experimental Data for Layer-Guided SH-APMs MHz surface skimming bulk wave (SSBW) Propagation orthogonal to x-axis of thinned (200 mm) ST-Q substrate IDT Face Coated Opposing Face to IDTs Coated Love wave and SH-APM SH-APM changes are both sensitive Love wave insensitive x-axis is d/λ with λ=idt period x-axis is d/λ with λ=idt period f (MHz) 45 APMs f (MHz) z v=v s Love waves McHale, et al, Appl. Phys. Lett., 2003, 82, September 2009 Newton, et al, Sens. Act., 2004, A109, F. Martin, PhD Thesis, Nottingham Trent University (2002) z
20 Love Waves and Higher Frequency Established QCM Sensor Principle Mass sensitivity Fundamental frequency Higher frequency Higher mass sensitivity Love Waves on a (Semi-) Infinite Substrate Controlling factor is guiding layer thickness x frequency z = d/λ l = df/v l S m = 1 v lim m 0 m v o fo ρ v l l d log dz e v z 0 Mass sensitivity Frequency Slope Factor Slope operating point z o d f Increasing frequency may or may not increase sensitivity 02 September
21 Love Waves and Frequency Hopping 6000 z 0 z 1 z 2 z 3 P hase S pee d, v m s a) c) b) d) dêl l Increasing frequency No Mode Change Transition a) Higher mass sensitivity Transition b) Lower mass sensitivity Mode Change Transition c) Lower mass sensitivity Transition d) Higher mass sensitivity 02 September
22 Sensor Research Examples 02 September
23 Examples 1 - Atmospheric Sciences Particulates 02 September
24 EP-SAW for Atmospheric Particulates Electrostatic Precipitation (EP) Charged particles deposited on collector Established principle for atmospherically borne microorganisms High (99-100%) efficiency System Design Air sampled via filter Particles ionised by N + 2 Particles collected onto path of biased metallised SAW i.e. Electrostatic precipitation 02 September 2009 Stanley, et al, NDT&E, 2005, 20,
25 Particulate Response with Bias Test Device Configuration LiNbO 3 - Rayleigh-SAW, λ ~ 45 µm 86 MHz & 253 MHz Test particulates via a NaCl aerosol: mono-disperse with ~ (2.0±0.1)µm Test particulates Not required for test Results Voltage of plate increased Bias induced precipitation to > 120 V in 20 V steps Phase changes Phase o V bias ~100 V Time / mins 02 September
26 Examples 2 - Biological Sciences Immune System and Vaccines 02 September
27 Peptides and T-Cells 1. Infection/virus broken into peptide fragments and presented on cell surface 2. Cytotoxic T-cells attach to peptides and read peptide sequence 3. If foreign, cell is killed by release of a cytotoxic chemical 4. Major histocompatability complex (MHC) antigens are responsible for the expression of peptides on the infected cell 5. Vaccine introduces peptide to the T-cell Aim is to find suitable peptides infected/antigen presenting cell LFA-3 ICAM-1 MHC class I CD22 B7 peptide T cell CD2 LFA-1 TCR T Cell Receptor CD8 CD45R CD28 02 September
28 Peptides and T-Cells 1. Infection/virus broken into peptide fragments and presented on cell surface 2. Cytotoxic T-cells attach to peptides and read peptide sequence 3. If foreign, cell is killed by release of a cytotoxic chemical 4. Major histocompatability complex (MHC) antigens are responsible for the expression of peptides on the infected cell 5. Vaccine introduces peptide to the T-cell Aim is to find suitable peptides Sensor Antigen Strategy LFA-3 presenting cell Make this the acoustic wave sensor Recognition layer is MHC protein Detect peptide specific binding Screen for suitable peptides (from the 1000 s that exist) with specificity and strong affinity for the MHC T cell CD2 ICAM-1 LFA-1 peptide MHC class I TCR CD8 CD22 CD45R Current State-of-Art B7 Cellular peptide-mhc assays yes/no and not real-time Sensitive, real-time and non-cellular based assay would assist vaccine development CD28 02 September
29 Flow Cell with Love Wave Screening Device Unfolded HLA A2 heavy chain α 1 α 2 α 1 α 2 Unfolded HLA A2 heavy chain β 2m protein binds to A2 and folds to create peptide specific binding cleft α 3 α 3 biotin biotin IDT streptavidin biotin biotin polystyrene layer LiTaO 3 substrate S1813 guiding layer IDT peptide peptide binding cleft α 2 α 1 α 2 α 1 binding cleft α 2 α 1 α 2 α 1 α 3 α 3 β 2 m α 3 β 2 m biotin biotin IDT streptavidin biotin biotin polystyrene layer LiTaO 3 substrate S1813 guiding layer IDT 02 September 2009 Stanley, et al, Analyst, 2006, 136,
30 Examples 2 - Biological Sciences Steroids and Sperm Motility 02 September
31 Molecularly Imprinted Polymers Target Applications (Liquid Phase) Recognition/selectivity via molecularly imprinted polymers (MIPs) Applications: monoterpenes, amino acids, topical steroids Tailor made enantioseparation materials MIP - Polymer Type Artificial Receptor Emil Fisher s Lock and Key (enzyme analogy) Functional Polymerisable Specific to the target analyte in terms of monomers derivative of their spatial Analyte and electronic environment analyte Analyte rebinding Imprinted cavity Synthesis of polymerisable template Chemical cleavage Acoustic Wave Sensor Polymerisation to fix cavity Recognition element (MIP) 02 September 2009 Percival, et al, Anal. Chem., 2001, 73, ; 2003, 75,
32 Synthesis of Nandrolone MIP Covalent Approach (Scheme 1) Phosgene, N 2 4-vinylphenol Nandrolone Nandrolone chloroformate Nandrolone 4vinyl phenyl carbonate Polymerisation Template cleavage Gives non-covalent recognition sites 02 September
33 Selectivity to Nandrolone QCM Coating Spin coated/cast layer Covalent imprinting strategy Response to Replicates One-shot screening Test data for 5 crystals Frequency Decrease / Hz Nandrolone Testosterone Epitestosterone Stanazolol f (Hz) concentration / ppm concentration (ppm) 02 September 2009 Percival, et al, Analyst, 2002, 127,
34 Sperm Motility Veterinary Artificial Insemination (VetAI) Sperm Quality Assessment & Detection Device (SQuADD) Time of flight/swim 5 MHz QCM (or use other AWS device) Frequency drop relative to reference Crystal pre-coated with sperm sticky material Experimental Sequence Stabilisation of signal in PBS Addition of sperm (arrow) Time of arrival data swim speed 02 September 2009 Newton, et al, Appl. Phys. Lett., 2007, 90, art
35 Examples 3 - Chemistry/Chemical Eng. Green Solvents 02 September
36 Ionic Liquids 7.0 Determining Physical Properties Room temperature ionic liquids (RTIL s) Green because non-volatile Millions of simple IL s, billions of binary ILs, Designer solvents Poorly characterised QCM Can measure density-viscosity product, but can also determine whether Newtonian via coupled frequency shift-bandwidth increase f=- B/2 Data Polydimethylsiloxane oil - known non-newtonian at higher molecular weights (ooo) Two ionic liquids [C 4 mim][otf] ( ) and [C4mim][NTf 2 ] ( ) Change in Frequency (khz) Change in frequency (khz) N N R O CF 3 S O N O S O CF 3 [C n mim][ntf 2 ](n=2,4,6,8,10) N (density x viscosity) 1/2 (kg m -2 s -1/2 ) N [C 4 mim][otf] O O S O CF Change in bandwidth (khz) 02 September 2009 McHale, et al, Anal. Chem., 2008, to be submitted. 36
37 Examples 4 Novel Devices Sectional guiding layers 02 September
38 Sectional Guiding Layers Guiding Layer IDT wave substrate IDT Mass Sensitivity Frequency Shift/ MHz S1813 guiding layer on quartz and LiTaO 3 with 100 MHz device AU deposited between IDTs to determine mass sensitivity S1813 Thickness/ µm Deposited Gold/ nm Sensitivity/ Hzng -1 mm Complete Sectional Guiding Layer Thickness/ µm Sensitivity/ Hzng -1 mm Guiding Layer Thickness/ µm 02 September 2009 Roach, et al, Sensors, 2007, 7, ; Roach et al. to be submitted
39 Liquid Sensing Water-glycerol mixtures Albumin adsorption 0.5 5% 0% 30% 50% 70% Glycerol 0.5 mg ml -1 BSA in PBS -2 Frequency Change/ Hz F re q u e n c y S h ift/ k H z Bare Sectional Complete Linear (Sectional) Time/ s Change (ρη ρη) 1/2 / Kg m -2 s -0.5 Frequency Change/ Hz Time/ s 02 September 2009 Roach et al. to be submitted
40 Other Research - Wetting Topography and surface chemistry 02 September
41 Selected Examples Slip and Slip Boundary Condition Nano-scale superhydrophobicity: Suppression of protein adsorption and promotion of flow-induced detachment, Lab on a chip (2008) accepted. Decoupling of the liquid response of a superhydrophobic QCM, Langmuir 23 (2007) Surface roughness and interfacial slip boundary condition for QCMs, J. Appl. Phys. 95 (2004) Contact angle-based predictive model for slip at the solid-liquid interface of a transverse-shear mode acoustic wave device, J. Appl. Phys. 94 (2003) Influence of viscoelasticity and interfacial slip on acoustic wave sensors, J. Appl. Phys., 88 (2000) Novel Applications of Superhydrophobicity Electrowetting of non-wetting liquids and liquid marbles, Langmuir 23 (2007) Implications of ideas on super-hydrophobicity for water repellent soil, Hydrol. Proc. 21 (2007) A lichen protected by a superhydrophobic and breathable structure, J. Plant Physiol. 163 (2006) Plastron properties of a super-hydrophobic surface, Appl. Phys. Lett. 89 (2006) Art Theory of Wetting Liquids shape up nicely, Nature Materials (Invited New & Views item) 6 (2007) Analysis of droplet evaporation on a super-hydrophobic surface, Langmuir 21 (2005) Contact angle hysteresis on super-hydrophobic surfaces, Langmuir 20 (2004) Materials Porous materials show superhydrophobic to superhydrophilic switching, Chem. Comm. (25) (2005) (See also Nature Highlight/News Quick change for super sponge Published on-line 20/7/05). Wetting and wetting transitions on copper-based super-hydrophobic surfaces, Langmuir 21 (2005) Topography driven spreading, Phys. Rev. Lett. 93 (2004) Art Dual-scale roughness produces unusually water repellent surfaces, Adv. Maters. 16 (2004) Intrinsically super hydrophobic organo-silica sol-gel foams, Langmuir 19 (2003) September
42 Hydrophobic grains water Optional PTFE metal contact substrate Foam heated (and cooled) prior to droplet deposition z z a b=0 b=- x L 02 September
43 Acknowledgements EPSRC (and BBSRC and EU and.) Research Group Funding of Research Doing the Work Dr Mike Newton, Dr Neil Shirtcliffe, Dr Fabrice Martin, Dr Simon Stanley, Dr Carl Evans, Dr Paul Roach, Ms Nicola Doy, Mr Shaun Atherton, Mr Steve Elliott + Mr Jeremy Simons Prof. Rees, Prof. Dodi, Dr Hughes Dr Percival Dr Gizeli and Dr Melzak Biological Sciences Atmospheric Sciences Biotechnology/Love Waves Prof. Thompson, Dr Lücklum Dr Hayward, Mr Ellis QCM and Slip Prof. Allen, Prof. Hardacre, Dr MacInnes, Ionic Liquids 02 September
44 Thank you for your attention
45 Love Waves Theoretical dispersion curve (Insertion loss is unchanged by an elastic guiding layer) Displacements for first three modes (z=1.3) P hase Spee d D isplacement o f laye r st mode 2 nd mode 3 rd mode z is dêl l 1 st mode 3 rd mode 2 nd mode x3êd 02 September
46 Substrate + Mass Guiding Layer Dispersion Curve Evolution of 1st SH-APM P hase S pee d, v m s APM s v=v s Love Waves Displacement x 3 êw 1000 v=v l dêl l Points = Anti-node moving from substrate to layer Displacement x 3 êd Solid dashed with increasing guiding 02 September
47 Dispersion and Group Velocity Guiding Layer Induces Dispersion Phase velocity v=fλ or v=ω/k Group velocity v g =dω/dk Group velocity is slope of the (ω, k) dispersion curve Example 0.25 µm polymer guiding layer on Quartz with w w n s Substrate speed Layer speed v a n d v g m s v g v k mm -1 1McHale et al, Sensitivity from group and phase., J. Appl.Phys. (2002) vol 92 2Martin et al, Experimental study of Love wave., IEEE Sensor Journal (2002) dêl l 02 September
48 Group Velocity Mass Sensitivity S m 1 1 ρld v v g = 1 ρ d l ( v v) g v g "Rigid" mass Mass sensitivity is fractional deviation of the phase velocity from the group velocity divided by mass per unit area due to the guiding layer Define a Group Velocity Sensitivity S g m = fo ρ v l l d log e dz v g z= z o»sm» a n d»sm g» m 2 k g Group sensitivity Phase sensitivity dêl l 02 September
49 Insertion Loss for Polymer Waveguide Cases considered 1. Wave-guide layer is viscoelastic 2. Mass layer deposited from liquid or from vacuum 3. Mass may be omitted (i.e. liquid phase sensitivity) Mass/liquid sensitivity can be derived for phase velocity & insertion loss Love Wave Insertion Loss Love Wave IL Sensitivity I nsertion L os s, I L d B m st mode 2 nd mode z I L S ensitivit y, Sm v d B m k g IL/10 5 for ωτ=10 IL for ωτ= z
50 Group Velocity Data - Solids Phase and Group Velocity 36 o XY LiTaO 3 Hardbaked photoresist Dotted curves are fits v m s Group speed Phase speed Mass Sensitivity S b 1 = v m ρ ld 1 v»s a m» a n d» S b m» m 2 k g g S a m 1 d log = ρ dx dêl l l e v x= d k -1» S g Sm m b» m 2 g dêl l S g m = 1 d log ρ l dx x= d »S a m» dêl m 2 kg -1 l e v g 02 September
51 Group Velocity Data - Liquids Deposition of Guiding Layer ST-Quartz + photoresist Poly (ethylene glycol) solutions = Phase velocity = Grp Velocity = Insertion loss Dotted = water Velocities (ms-1) Insertion loss (db) Sensitivity at Operating Points Phase and group velocities d/ λ ldt -65 v p/v p (ρ PEG η PEG ) (ρ w η w ) (kg.s -1.m -2 ) vg/vg (ρ PEGη PEG) (ρ wη w) (kg.s -1.m -2 ) 02 September
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