ABOUT OMICS GROUP CONFERENCES
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1 ABOUT OMICS GROUP O M I C S G R O U P I N T E R N A T I O N A L T H R O U G H I T S O P E N A C C E S S I N I T I A T I V E I S C O M M I T T E D T O M A K E G E N U I N E A N D R E L I A B L E C O N T R I B U T I O N S T O T H E S C I E N T I F I C C O M M U N I T Y. O M I C S G R O U P H O S T S O V E R L E A D I N G - E D G E P E E R R E V I E W E D O P E N A C C E S S J O U R N A L S A N D O R G A N I Z E O V E R I N T E R N A T I O N A L C O N F E R E N C E S A N N U A L L Y A L L O V E R T H E W O R L D. O M I C S P U B L I S H I N G G R O U P J O U R N A L S H A V E O V E R 3 M I L L I O N R E A D E R S A N D T H E F A M E A N D S U C C E S S O F T H E S A M E C A N B E A T T R I B U T E D T O T H E S T R O N G E D I T O R I A L B O A R D W H I C H C O N T A I N S O V E R E M I N E N T P E R S O N A L I T I E S T H A T E N S U R E A R A P I D, Q U A L I T Y A N D Q U I C K R E V I E W P R O C E S S.
2 ABOUT OMICS GROUP CONFERENCES OMICS Group signed an agreement with more than 1000 International Societies to make healthcare information Open Access. OMICS Group Conferences make the perfect platform for global networking as it brings together renowned speakers and scientists across the globe to a most exciting and memorable scientific event filled with much enlightening interactive sessions, world class exhibitions and poster presentations Omics group has organised 500 conferences, workshops and national symposium across the major cities including SanFrancisco,Omaha,Orlado,Rayleigh,SantaClara,Chicago, Philadelphia,Unitedkingdom,Baltimore,SanAntanio,Dubai,Hy derabad,bangaluru and Mumbai.
3 ULTRAFAST DYNAMICS EXPLAINING PH PROBE MECHANISM IN BORONDIPYRROMETHENE BENZIMIDAZOLE COMPOUND G Ö K H A N S E V I N Ç, B E T Ü L K Ü Ç Ü K Ö Z, H A L I L Y ı L M A Z, G Ö K H A N Ş I R I K Ç I, H. G U L Y A G L I O G L U, M U S T A F A H A Y V A L ı, A Y H A N E L M A L I
4 OUTLINE Motivation Optical Chemical Sensors Investigated Material Synthesis Experiments Results and Discussion
5 MOTIVATION PH Sensors Applications Chemical Processing Pulp and Paper Food and Beverage Pharmaceutical Biomedical Industrial Requirements for Biomedical and Industrial Applications real time measurement continuous monitoring A perfect solution Optical Chemical ph Sensors 1 1 Anal. Chem. 2014, 86, 15 29
6 OPTICAL CHEMICAL SENSORS SENSES OF ELECTRONICS 1 EYES AND EARS CAPABLE OF SEEING AND HEARING BEYOND THE HUMAN PERCEPTION 1 Optical sensors are based on reagents that change their optical properties on interaction with the analyte of interest. 2 The most commonly measured optical properties Absorption, Fluorescence intensity Decay time Reflectance Refractive index Light scattering Light polarization Fluorescent ph indicators offer better selectivity and sensitivity than absorption-based ph indicators. 2 1 Orellana, G. In Optical Chemical Sensors. Proceedings of the NATO Advanced Study Institute on Optical Chemical Sensors; Baldini, F., Chester, A. N., Homola, J., Martellucci, S., Eds.; Springer: Netherlands, 2006; pp Dorota Wencel, Tobias Abel, and Colette McDonag, Anal. Chem. 2014, 86, 15 29
7 MATERIAL Properties of Borondipyrromethene (BODIPY) dyes High fluorescence quantum yield 1 High extinction coefficient Narrow absorption and emission bands 2 Fine-tuned spectroscopic and physical properties Applications Optical engineering Fluorescence imaging Fluorescent chemo sensors (such as ph probe) BODIPY scaffold 1 L. Gai, et al, Sens. Actuators B 182 (2013) F.L.P. Arbeloa, et al, Int. Rev. Phys. Chem. 24 (2005) Y. Ando, et al, Sens. Actuators B 121 (2007) W. Qin, et al, ChemPhysChem 6 (2005) T.Werner, et al, Fresenius J. Anal. Chem. 359 (1997)
8 MATERIAL Properties of Benzimidazole compound A heterocyclic aromatic organic compound Consists of a fusion of benzene and imidazole Usually used as ligand for transition metal complexes Protonation property due to presence of pyridinic nitrogen atom in benzimidazole Benzimidazole
9 SYNTHESIS a: 2,2-dimethylpropane-1,3-diol, p-toluen sulfonic acid, toluen, Ar, refluks, 24h, %50; b: 2,4-dimethyl-3-ethylpyrrole, trifluoroacetic acid, CH 2 Cl 2, rt, 24 h.; c: 2,3-dichloride-5,6-dicyano-1,4-benzoquinone (DDQ), 45min.; d: Diisopropylethylamine (DIPEA, 5min) BF 3.OEt 2, 2h.(%20); e: CH 2 Cl 2 :H 2 O, trifluoroacetic acid, rt, 6h.(%90); f: o-phenylenediamine, p-tsoh, DMF, 3h. Reflux (%60)
10 EXPERIMENTAL TECHNIQUES Linear absorption spectroscopy Emission spectroscopy Femtosecond transient absorption spectroscopy
11 EXPERIMENTAL RESULTS 1) 1) S0 S1 2) 2) out of plane vibrations of the aromatic skeleton 3) 3) S0 S2 (a) Absorption spectra of PROBDP in different solvents. (b) Emission spectra of PROBDP. (c) Absorption spectra of ALDBDP. (d) Emission spectra of BIMBDP. (e) Absorption spectra of BIMBDP. (f) Emission spectra of BIMBDP.
12 EXPERIMENTAL RESULTS Absorption and emission spectra of BIMBDP in various ph intervals (dye concentration was 2x10-5 molar in 5:1 methanol-water mixture at excitation wavelength of 525 nm).
13 FEMTOSECOND TRANSIENT ABSORPTION SPECTROSCOPY EXPERIMENT Intensity (au) Pump pulse Intensity (au) Wavelength (nm) spectrometer Wavelength (nm) Probe pump pulse comes after pump Intensity (au) Nonlinear absorption Intensity (au) sample Linear absorption Wavelength (nm) Wavelength (nm)
14 FEMTOSECOND TRANSIENT ABSORPTION SPECTROSCOPY EXPERIMENT Pump pulse 500Hz Intensity (au) Wavelength (nm) spectrometer sample Probe pulse Intensity (au) beam dump 1000Hz Wavelength (nm)
15 Pump pulse 500Hz Intensity (au) Intensity(au) Wavelength (nm) spectrometer Wavelength (nm) Intensit ty(au) Wavelength (nm) Probe pulse comes after the pump pulse 1000Hz Intensity (au) Wavelength (nm) sample beam dump Intensity(au) Wavelength (nm)
16 FEMTOSECOND TRANSIENT ABSORPTION SPECTROSCOPY EXPERIMENT Example Transient Absorption Data SA SA ESA ESA SA SA
17 EXPERIMENTAL RESULTS Femtosecond transient absorption spectra as a function of time for (a)unprotonated and (b)protonated compound of BIMBDP
18 EXPERIMENTAL RESULTS 532 nm probe wavelength Femtosecond transient absorption spectra of BIMBDP compound in (5:1 MeOH-H 2 O) with different ph value. The Fig. inset indicates fast decay of transient band corresponding to LUMO energy level of benzimidazole.
19 DENSITY FUNCTIONAL THEORY CALCULATION Energy levels and frontier molecular orbital distributions of the novel BODIPY compound in protonated and neutral states respectively
20 PROPOSED MECHANISM Proposed mechanism for fluorescence quenching of BIMBDP in acidic media attributed by d-pet a) fluorescence mechanism b) quenching mechanism
21 RESULT AND DISCUSSION We have designed and synthesized new BODIPY compounds Absorption and emission properties were investigated as a function of H + concentration Drastic fluorescence quenching was observed upon protonation Photo-induced electron transfer was proposed as an underlying mechanism Underlying mechanism was proved by DFT calculation and ultrafast transient absorption experiments
22 THANK YOU FOR YOUR PATIENCE
23 LET US MEET AGAIN We welcome all to our future group conferences of Omics group international Please visit:
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