General Linear Model (Chapter 4)
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1 General Linear Model (Chapter 4) Outcome variable is considered continuous Simple linear regression Scatterplots OLS is BLUE under basic assumptions MSE estimates residual variance testing regression coefficients and confidence intervals Centering and standardizing variables Regression coefficients with continuous versus categorical predictors
2 Cholesterol predicting blood pressure A toy example: Suppose we randomly select ten patients from a clinic and measure their blood pressure and cholesterol levels at their visit. Investigators are interested in the relationship between total blood cholesterol and blood pressure (the ratio of systolic/diastolic). 10 BP ratios: (1.51, 1.63, 1.52, 1.43, 1.58, 1.5, 1.66, 1.55, 1.6, 1.49) 10 cholesterol levels: (190, 230, 175, 200, 245, 195, 300, 210, 235, 290) Scatterplot of BP ratios versus cholesterol levels. What can we see?
3 Simple Linear Regression Estimate the Expected value (mean value) of Blood Pressure ratio given a particular value of cholesterol. Assume linear model for the mean: Assume the error is additive with mean zero: So, we have in general matrix form:
4 Ordinary Least Squares (OLS): Estimation
5 Hypothesis test
6 Hypothesis test (cont.)
7 SAS output proc reg data=bp; model bp = chol; run; Explanation of the outputs: Model: MODEL1 Dependent Variable: bp Number of Observations Read 10 Number of Observations Used 10 Analysis of Variance Sum of Mean Source DF Squares Square F Value Pr > F Model Error Corrected Total Root MSE R-Square Dependent Mean Adj R-Sq Coeff Var Parameter Estimates Parameter Standard Variable DF Estimate Error t Value Pr > t Intercept <.0001 chol
8 Stata output. reg bp chol Source SS df MS Number of obs = F( 1, 8) = 2.67 Model Prob > F = Residual R-squared = Adj R-squared = Total Root MSE = bp Coef. Std. Err. t P> t [95% Conf. Interval] chol _cons Explanation of the outputs:
9 Interpretation of output Typically main target of interest is whether regression coefficients are significant. What is conclusion? Is testing the intercept in the current model interesting? How do you interpret estimate for chol. How to estimate the expected increase in BP for a 10 unit increase in cholesterol? How to create 95% confidence interval for parameter estimates? Know how to do it by hand. Question: which t-value to use: a , b. qt(.975,8)=2.306, c. qt(.975,9)=2.262, d. qt(.975,1)=12.706, e. 1.96? In SAS add options to the model statement after /: model bp = chol / clb alpha =.05; What are the different parts of the ANOVA? F-test, MSE What is the standard deviation of BPratio? What would the Pearson correlation coefficient be between chol and BP? What would its p-value be?
10 Standard Deviation vs. Standard Error Suppose n samples with sample mean x Standard deviation: x 2 i x SD n 1 tells us the distribution of individual values around the mean. (If we draw another sample from the same population, it will likely have a value within x ± 3SD) Standard error of the mean: SD SE n tells us the distribution of the means, i.e., it is the standard deviation of sampling distribution of the means. (If we draw another set of samples from the same population, the mean of the new samples will likely be within x ± 3SE) Standard error of the regression: estimate of the standard deviation of the underlying errors. Recall the estimated standard error in OLS ˆ 2 MSE
11 Sum of Squares Sum of Squares: n Total Sum of Squares (TSS): TSS y 2 -- total variability of the i y outcome Model Sum of Squares (MSS): -- variability explained MSS by the model yˆ 2 i y Residual Sum of Squares (RSS): n -- variability not explained by the model RSS y yˆ TSS = MSS + RSS Estimate of variance of ε: RSS/(n-p) (Mean Square Error, MSE) Coefficient of determination, R 2 = MSS/TSS Interpretation: the proportion of the total variability of the outcome (TSS) that is accounted for by the model (MSS). statistically significant predictor does not necessarily suggest large R 2 Adjusted R 2, 1-(n-1)(1- R 2 )/(n-p), adjust for the number of predictors in a model i1 n i1 i1 2 i i
12 Fitted regression line bp chol
13 Fitted regression line with confidence interval We can also obtain a confidence interval for the fitted means. bp chol Stata: twoway lfitci SAS: Proc sgscatter; plot / reg=(clm); run;
14 Fitted Mean Fitted mean: where For given covariates X=x 0 : 95% CI: 1 ˆ ˆ T T Y X X X X X Y T 1 X X X X T is called the hat matrix (projection matrix). Y~N Xβ, σ 2 H ˆ ˆ 2 T 1 Var Y x Var x x X X x x ˆ t x X X x 2 0 ˆ /2, n p 0 0 T 1 Interpretation of CI: if we repeat the study for a large number of times using the same values of X, 95% of time the observed CIs would bracket the true mean response, E(Y x 0 ).
15 Predicted Mean For a future observation (not included in the model fitting): Y X * ˆ Y ~N Xβ, σ 2 I + H For given covariates X=x 0 : 95% CI: * ˆ 2 T 1 Var Y x Var x x X X x x ˆ t ˆ 1 x X X x 2 T 1 0 /2, n p 0 0 The CI for predicted mean is wider than that for fitted mean. 1
16 Centering and standardizing variables What happens if center the X variable, that is, create X i = X i X and redo the OLS regression this time of Y on X i? How do the estimates and their standard errors change. How do the elements of the ANOVA change? What about the R 2? What is interpretation of confidence interval for β 0. What about if we standardize the X variable, i.e. X i = X i X /sd(x)? How to interpret? What about if we standardize both the X and Y variables? How to interpret?
17 Centering predictor 1. The predictor is centered: Root MSE R-Square Parameter Standard Variable DF Estimate Error t Value Pr > t Intercept <.0001 chol_c bp 1.55 bp chol chol (centered)
18 Standardizing predictor 2. The predictor is standardized: Root MSE R-Square Parameter Standard Variable DF Estimate Error t Value Pr > t Intercept <.0001 chol_std bp 1.55 bp chol chol (standardized)
19 Standardizing both outcome and predictor 3. Both the outcome and predictor are standardized:: Root MSE R-Square Parameter Standard Variable DF Estimate Error t Value Pr > t Intercept E chol_std bp bp (standardized) chol chol (standardized)
20 Continuous vs. categorical predictor continuous predictor X: β 1 interpreted as slope of line β 0 is the intercept, which corresponds to the mean outcome when X = 0. categorical predictor X: create dummy(0/1) variables β 1 interpreted as mean difference in outcome comparing a specific group to the reference group β 0 is interpreted as mean of outcome in reference group
21 Categorical predictor For our BP ratio-chol example, suppose we also have Gender information. Create a 0-1 variable where 1 indicates Male and 0 indicates Female. Regress BP ratio on Gender:
22 SAS output: proc reg data=bp; model bp = gender; Run; Analysis of Variance Sum of Mean Source DF Squares Square F Value Pr > F Model Error Corrected Total Root MSE R-Square Dependent Mean Adj R-Sq Coeff Var Parameter Estimates Parameter Standard Variable DF Estimate Error t Value Pr > t Intercept <.0001 gender What is 1.54? What is ?
23 Stata output: Source SS df MS Number of obs = F( 1, 8) = 0.13 Model Prob > F = Residual R-squared = Adj R-squared = Total Root MSE = bp Coef. Std. Err. t P> t [95% Conf. Interval] gender _cons
24 Testing gender using a 2 sample t-test gender N Mean Std Dev Std Err Minimum Maximum Diff (1-2) gender Method Mean 95% CL Mean Std Dev 95% CL Std Dev Diff (1-2) Pooled Diff (1-2) Satterthwaite Method Variances DF t Value Pr > t Pooled Equal Satterthwaite Unequal Equality of Variances Method Num DF Den DF F Value Pr > F Folded F How do these results match up with those from the regression?
25 Technically we are still estimating a line What does the intercept represent? What does the slope represent?
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