12V 20 W. 2 1~8 Narrow Band Interference Filters Additional Aspirate Read. Contrast 5. Header. Work area. Message bar.

Size: px

Start display at page:

Download "12V 20 W. 2 1~8 Narrow Band Interference Filters Additional Aspirate Read. Contrast 5. Header. Work area. Message bar."

Cornelia Weaver
5 years ago
Views:

1 12V 20 W ~8 Narrow Band Interference Filters Additional Shift Leds Shift + Reset 0.5 Delete 1.5 ml Flow Cell Aspirate Read Rinse Led Exit Enter Reset Contrast 5 Header Work area Message bar Function keys Run Test Screen

2 6 Start Up Help Home Back Main Menu Soft Ware Basics 6 0~9 Enter 0.5 SEC Calib 700 ~340 Pri wavelen Calibration Screen 7 On 8 Soft Ware RX Monza Enter Flow Cell Main Menu

3 Start up and perform daily checks Perform gain and pump calibration Select test from the test menu, edit assay parameters if required and press save Enter The Calibration Detail S In The Calibration Menu And Press Save Prepare samples and reagents in test tubes Incubate reaction at the required temperature Place aspiration tube into the reaction mixture Press aspiration switch to begin measurement The absorbance value will be calculated according to the test parameters, and will calculate results based on the stored calibration Retrieve results of samples, calibration and QC data ي جد 192 بزنامج مصنفة في ستة صفحات جشء من ىذه البزامج مبزمج من قبل شزكة راند كس الجشء األخز مفت ح لبزمجتو من قبل مستثمز الجياس 9

4 None 12 1 Point 2 Point PT-S Blank Rate A Absorbance Assay Mode ~ ~40 SEC 999 ~ Delay Time 999 ~15 Read Time Unit

Lim 100 ~ 0 3.0000 ~ -0.25 Min Rx ABS 3.

R3 VOL 999999 ~ -999999 Slope A 999999 ~

5 Format.9 1~99 Replicate: 10MM Flow Cell Cuvette 0~ Ref- Low 0~ Ref- High 0~ Min Lin Lim 0~ Max Lin Lim 100 ~ ~ Min Rx ABS ~ Max Rx ABS ~ Min Rgt ABS ~ Max Rgt ABS 9999 ~ 200 µl ASP VOL 999 ~ 0.1 µl Sample VOL 6500 ~ 1 µl R1 R1 VOL 6500 ~ 0 µl R2 R2 VOL 6500 ~ 0 µl R3 R3 VOL ~ Slope A ~ Intercept B Type Of 2 RX Monza Programming Calibration Parameters Parameters Main menu K-Factor Linear Repl 192 Curve

6 Page Flag Param S0~S11 STD Factor ABS Conc S0 ~S1 ± Fat Dev% Repl Lim 0 ~ 'R' 0 ~ ~100 Curve Param Print Save Page Down Up Print Save Run Calib Enter the concentrations of the calibrators in the S0 and S1 fields

7 3 3 ID ~ Mean ~ SD ~ C2 C2 Mean ~ C2 C2 2SD ~ Mean ~ SD.6 QC

$C2\\ Mthly\Daily Calib Data\$

8 ,C2 And QC Daily Graph QC-Daily Data QC-Monthly Graph QC-Monthly Data Accuracy Of Analysis Precision Of Analysis QC- Daily Graph- QC ±3SD SD ±2SD QC N Mean Value X Stanard Drviation SD CV% R Print C2 C2\\ Mthly\Daily Calib Data\ Graph QC-Daily Data

$Print C2 C2\\ Mthly\Daily Verify Exclude\$

9 SD Mean 10 Stanard Drviation Mean Value N X SD CV% R SR.NO. Analysis Time Control Results Remark Flag Print C2 C2\\ Mthly\Daily Verify Exclude\ Include Save QC-Monthly Graph

$CV% R Print C2 C2\\ Mthly\Daily$

10 QC 31 QC ±3SD SD ±2SD QC N Mean Value X Stanard Drviation SD CV% R Print C2 C2\\ Mthly\Daily Calib Data\ Graph QC-Monthly Data

$Print C2 C2\\$ $Mthly\Daily Verify$ : Run Test Screen

11 QC SR.NO. Analysis Date Control Results Remark Flag Print C2 C2\\ Mthly\Daily Verify Exclude\ Include Save : Run Test Screen Actual temperature attained after flow cell/cuvette blanking Sample Information Sample Information

Assay Calibration Sample Analysis Quality

12 Assay Calibration Sample Analysis Quality Control Check Calibration Pre Wavelen Sample VOL Factor Replicates SEC ABS Net Sample ABS Parameter Assay Mode ABS-S0 ASP VOL PRI ABS R2 VOL Sample Number Sample ABS SEC Wavelen Programmed R1VOL Real-Time ABS Sample Blank ABS Calculated % Linearty Sample Result With Flag Water Blank 1 Absorbance Assay Mode Rerun Water Blank Ok 2 Water Blank Fail Sample Aspiration 3

13 Aspirate Replicate M Of N ASP Of Sample No ##### SKIP Rerun Real Time Absorbance RX curve 4 Skip Print Rerun RX Data Reaction Data Real Time Primary Absorbance 5 Time Real Time Secondary Absorbance Real Time Absorbance

14 Page Down RX Data PAGE UP Print Rerun Rerun RE-Running Samples Aspirate Replicate 2 Of 3 Of Sample No 10 Skip Skipping Sample Analysis Skip Calibration 6

15 Full Run Test Calib RX Curve End Of Rerun Curve Cal Data Calibration Rerun RX Curve Reject Accept Curve\Data Sample Analysis 7

16 Flow-Cell Mode Run Test Screen Enter Aspirate Replicate M Of N Of Sample Number ######### Cuvette Mode Press Read Key For Replicate M Of N Of Sample Number ####### 6 Manual Entery Of Sample Number Aspirate Running Sample In Flow Cell Mode Read Running Sample In Cuvette Mode Read Do You Want To Add R2 Reagent? If Yes Press 1, If No Press 0 Read QC Flag RX CURVE\ RX Data Rerun Skip QC Analysis 8

$RX Curve\$ Flags QC

17 RX Curve\ Skip Flags QC QC, Run QC Rerun RX Data Using The Flow Cell

Brooklyn College Department of Chemistry

Brooklyn College Department of Chemistry Instrumental Analysis (Chem 42/790) Atomic Absorption Spectroscopy An atomic absorption spectrometer is used in this experiment to analyze a copper-base alloy for