RA-520N/RA-500N/RA-410
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1 Refractometer/Brix Meter RA-520N/RA-500N/RA-410 High-performance built-in thermostatic feature Compatible with PC card (RA-520N only) Interface to Barcode reader for easier input of sample ID CE marked for product safety ISO 9001 for quality assurance GLP/GMP conformed ISO environmental management systems
2 Refractometer RA-520N/RA-500N Built-in temperature control High performance wide range Introduction The RA-520N/RA-500N determines the refractive index of a liquid sample by detecting critical angle of optical deflection from the sample and prism. Refractometry is now commonly used for quality control in foods and beverage industries. Measurement of refractive index by RA-520N ranges from 1.32 to 1.58 with resolution while RA-500N from 1.32 to 1.70 with resolution. Both models reproduce instrumental precision check and its record by printout to GLP compliance. They are designed and manufactured in full conformance to ISO standard and CE marking both EMI and LVD requirements. Features of RA-520N 1) Refractive index with high precision can be obtained by resolution of nd and Brix. 0.01%. 2) Measurement results are highly reliable by border temperature between sample and prism detected with ±0.05¼C precision thanks to the built-in thermostatic control. 3) The PC card multiplies memory space so that more measurement data or userõs conversion tables can be stored. 4) Concentration conversion table of isomerized sugar is available by use of optional PC card. 5) Measurement results can be obtained by easy operation in a short time (about 4 seconds) for refractive index, Brix or concentration. 6) Rigid LED light source eliminates replacement of conventional light bulb. 7) The prism is made of sapphire which is chemically resistant, rigid and of high thermal conductivity. 8) Up to 10 operatorõs name can be registered. 9) Data storage and printout are fully GLP conformed by additional functions including check measurement and recording history of calibration. 10) The built-in thermal control shortens time to reach selected temperature, thus improving setup time and workload. 11) More variety of applications is offered by a full line of peripherals as well as by the combined measurement with Density/Specific gravity meter (DA-520/DA-510/DA-505/DA- 500) so that density and refractive index can be determined simultaneously. 12) Data capture software SOFT-CAPE, when installed in userõs personal computer, can download the data into Microsoft Excel or store in CSV format. 13) Interface to Barcode reader for easier input of sample ID, etc. Features of RA-500N 1) Wide range of refractive index from nd 1.32 to 1.70 makes measurement of samples with high RI possible. 2) The built-in thermal control from 15 to 70¼C can determine refractive index at high temperature. 3) Measurement results can be obtained by simple operation in a short time (about 2 seconds) for refractive index, Brix or concentration. 4) The LED light source eliminates replacement of conventional light bulb. 5) The prism is made of sapphire which is chemically resistant, rigid and of high thermal conductivity. 6) Up to 10 operatorõs name can be registered. 7) Data storage and printout are fully GLP conformed by additional functions including check measurement and recording history of calibration. 8) The built-in thermal control shortens time to reach selected temperature, thus improving setup time and workload. 9) More variety of applications is offered by a full line of peripherals as well as by the combined measurement with Density/Specific gravity meter (DA-520/DA-510/DA-505/DA- 500) so that density and refractive index can be determined simultaneously. 10) Data capture software SOFT-CAPE, when installed in userõs personal computer, can download the data into Microsoft Excel or store in CSV format. 11) Interface to Barcode reader for easier input of sample ID, etc. 1
3 RA-520N/RA-500N/RA-410 Application of Refractometer RA-520N, RA-500N 1) Petroleum products Measurement of refractive index is applied in quality control of light oil, kerosene, gasoline, etc., and also applied to quality check in chemical process of cyclohexene, styrene, benzene, toluene or xylene. 2) Fats and oil Edible oils like soybean oil, cotton oil, sesame oil, olive oil, palm oil, coconut oil, etc. are quality controlled by measuring refractive index at 25¼C 3) Perfume and pharmaceutical products Perfume and material for pharmaceutical products are expensive by weight, and purity of those materials is critical to their cost. Thus, the purity is determined by measuring refractive index, which requires a small amount of sample, being advantageous feature of Refractometer. 4) Fruits The most important factor in quality determination of fruits other than shape, color or taste is the degree of fructose in the apple, orange, grape, pear, melon, etc. Refractometry is applied not only in quality control but determines their harvest season. 5) Honey and jam Production control or quality determination of honey, jam, marmalade, etc. is performed by Refractometer or Brix meter. Refractometry is also applied to quality check of raw materials for starch syrup and liquid sugar in foods industry. 6) Beverages Soft drinks including soda and fruits juice contain not only sucrose but other substances like flavor, citric acid, pectin, etc. All of these ingredients are collectively called "Solid", and its quality or production control is determined by Brix. 7) Cooking sauce Quality of liquid sauce like steak sauce, soy sauce, ketchup or dressings depends on inspecting its concentration of raw material as well as proportion of ingredients in process. Key factor for these checks is the Solid portion determined by Refractometer or by Brix meter. 8) Quenching oil, roll oil, cutting oil, etc. To check concentration of these cooling oils is critical for improving work efficiency and quality of mechanically processed products. Quality and production control are perfectly managed by input of relation of refractive index and concentration of these oils. 9) Anti-freeze fluid Anti-freeze fluid used in the automobile or other combustion engines consists mainly of ethylene glycol or propylene glycol. The higher concentration of the fluid, the less likely the engine is frozen in winter time. Needless to say, to control the concentration of such fluid is very important in freezing cold area. 10) Production of electronic parts Chemical liquids used in production line for electronic parts need severe quality check by refractometry of their concentration, which leads to the quality of finished products. 11) Other applications Decontaminating chemical like ethyl alcohol or disinfectant like hydrogen peroxide, etc. Especially solution used in food processing requires control of concentration to a constant level, which is easily and precisely performed by Refractometer with its built-in conversion processor. Concentration conversion Calibration curve is pre-determined and plotted, using a plural number of liquid of which concentration is known. When refractive index of a sample of which concentration is unknown is measured, the results are converted to unit like % or ppm. Measurement refractive index Concentration Calibration curve % ppm mg/g mg/ml refractive index 2
4 RA-520N Principle of Measurement of Refractive index Sample Any visible light changes its direction when it passes a material with higher refractive index (RI) to that with lower refractive index. As the incident ray b increases, the refractive angle a will increase in accordance with the Snell's Law, and when the refractive angle a reaches the critical angle (=90û), total refraction will occur at the boundary between the prism and the sample. In actual measurements by our Refractometers, the light source, the prism (nd=1.768) and the line sensor CCD are placed as shown in the right figure, and refractive index can be determined from the CCD address of the boundary (Critical angle) between the ÔLightÕ and ÔDarkÕ area on the line sensor by detecting the intensity of the reflected light with the CCD sensor. Prism Light source LED Line sensor CCD Examples of Measurement with the Refractometer CCD address Intensity of reflected light 1) Refractive index of Chemical products 2) Brix measurement on Soft drinks 3) Concentration of chemical products 3
5 Technical tip on thermostatic control RA-520N/RA-500N/RA-410 Detection unit (CCD) Sample stage Receiving convex lens Prism Emitting convex lens Polarization filter Interference filter L E diode Since refractive index largely depends on temperature, it is important to maintain a constant degree in the measurement. Temperature control in RA-520N/RA-500N is made by the thermal control unit on both sides of prism so that the temperature at the border of sample and prim can be maintained to the preset degree. Thus, the measurement of refractive index by RA series is most reliable and assured at all times. Temperature control unit Cooling fin Specification of Refractometer Type and Model name Measuring method RA-520N Refractometer RA-500N Refractometer Detection of critical angle of refractive Na-D line (589.3nm) nd: Ð nd: Ð Measuring range Brix:0.00 Ð 85.00% (ICUMSA conformed) Ð % (out of ICUMSA) * Soluble solid converted at 20 C based on conversion table at the 16th session of ICUMSA 1974 Brix: 0.00 Ð 85.00% (ICUMSA conformed) Ð % (out of ICUMSA) * Soluble solid converted at 20 C based on conversion table at the 16th session of ICUMSA 1974 Accuracy Repeatability Resolution Temperature control Temperature range Temperature precision Temp. resolution Minimum sample Measuring time Display Sampling Method Temperature compensation Conversion of refractive index to concentration Brix conversion Solid conversion Statistical computation Recalculation Printer PC card Interface Connection to KEMÕs density meter Liquid contact material Ambient condition Power source Power consumption Dimension Weight nd: ± within 1.32 Ð 1.40 nd: ± ± within 1.40 Ð 1.58 Brix: ±0.03% (0.00 Ð 85.00%) Brix: ±0.1% (0.0 Ð 85.0%) nd: ± nd: ± Brix: ±0.01% Brix: ±0.1% nd: nd: Brix: 0.01% Brix: 0.1% Temperature at refractive border controlled by Pertier semi-conductor 15 Ð 50 C 15 Ð 70 C ±0.05 C ±0.1 C 0.01 C 0.1 C Approx. 0.2mL 4 seconds (depends on sample) 2 seconds (depends on sample) dot LCD with backlight Displays nd, Brix(%), concentration(%, ppm, mg/g, mg/ml, mol/l, etc.) 1) Manual by dripping pipette 2) Combination of auto sampling unit and flow cell (option) 10 methods can be stored with individual measurement conditions in file Brix: automatic correction by stored conversion table Sample in general: input coefficient for correction Automatic by input of related formula of concentration and refractive index Or, by input of the table of concentration vs. refractive index Standard By optional PC card Not available Automatic output of mean value, standard deviation (SD) and relative SD Yes IDP-100 ink ribbon impact dot printer (option) 1) Stores data or parameters 2) Upgrade version by PC card RS232C for personal computer and printer at the same time and Serial interface for Multiple Sample Changer for full automation RA series can be connected to KEM density/specific gravity meter for combined measurement for both refractive index and density. Sapphire prism, SUS-316 stainless steel, PTFE for FCU-520 flow cell Temperature: 5 Ð 35 C, Humidity: less than 85% RH (no condensation) AC100V Ð 240V, 50/60Hz 60W 288(W) 468(D) 247(H) mm 9kg approx. 1) not available 2) upgrade version by PC card 4
6 Systems Standard system IDP-100 Printer # Connecting cable IBM PC/AT Series RA-520N Refractometer # Density/Refractometer connecting kit F DCU-550N Auto clean and sampling unit # Density/Refractometer connecting kit F CHD-501N Multi-sample changer RA-500N Refractometer # Connecting kit for ASU ASU-510 Auto samplimg unit # Density/Refractometer connecting kit C DA-520/DA-510 DA-505/DA-500 Density/specific gravity meter 5
7 RA-520N/RA-500N/RA-410 Optional data capture software for RA-520N/RA-500N Application Measurement data of Refractometer can be downloaded to Microsoft Excel in userõs personal computer or directly filed in CSV format. Note: User's PC must have Windows Excel installed. Specifications on the Multiple Sample Changer and the Auto Clean and Sampling Unit Type and model: Number of vials Minimum sample required Sample bottle Sample position CHD-501N Multiple Sample Changer Up to 30 (option: 48) 10mL (5mL for the special vial) 20mL vial (8mL for 48-vial system) Turntable system DCU-550N Auto Clean and Sampling Unit Only one sample 20mL vial (Option: 8mL vial) Fixed Sampling system Drain system Pressurized sampling with the built-in pump (option: Suction system) Pressurized draining (Option) Suction system (Option) Sample-returning (to vial) system Auto sequential events Auto calibration Program sequence Control Sampling, drain, rinse (two kinds of solvent), purge dry With air and water or with two kinds of reference materials Sequential events can be programmed on the measuring unit. a) Auto : Start operations on the measuring unit b) Manual : Manually operate by key entry Display Ext. communication Connectable unit 20 digits by 2 lines LCD with a backlight Simplified serial interface via the connecting cable (# ) Density meters: DA-520, DA-510, DA-505, DA-500 Refractometers: RA-520N, RA-500N General-purpose concentration meters: WBA-505, WBA-505B Main supplied parts Ambient conditions Vial (20mL): 30 pcs, Packing: 100 sheets, Cap: 30 pcs, Nozzle packing: 6 pcs 1) Temperature: 5 to 35ûC, 2) Humidity: less than 85%RH (No condensation) Power source Power consumption VAC/ VAC, Approx. 40W 50/60Hz Dimension Weight 582(W) x 402(D) x 392(H)mm Approx. 17kg 239(W) x 310(D) x 342(H)mm Approx. 9.5kg 6
8 RA-520N/RA-500N/RA-410 Brix Meter RA-410 Specification of Brix meter Introduction The RA-410 Brix Meter measures refractive index of liquid sample and converts it to Brix value or isomerized sugar concentration. Hard copies of measurement data can be output by the optional printer, and such data can even be downloaded to userõs personal computer for further data processing. Feature 1) The instrument is fabricated into drip-proof structure. 2) Reproducibility of measurement results is Brix ±0.01%. 3) Measurement temperature is automatically compensated. 4) Measurement results can be obtained by easy operation in a short time. 5) Light source is LED, thus eliminating replacement of conventional light bulb. 6) The prism is made of sapphire which is chemically resistant, rigid and of high thermal conductivity. 7) Highly reliable measurement is made possible by temperature detection between the sample and prism. Type and Model name Measuring method Measuring range RA-410 Brix Meter Measuring method Detection of critical angle of Na-Dline ( 589.3nm ) 1) Brix: 0.00 to 85.00% (ICUMSA conformed) to 100% (out of ICUMSA) Soluble solid converted at 20ûC based on conversion table at the 16th session of ICUMSA ) HFCS42: 0.00 to 76.00% (ICUMSA conformed) to 100% (out of ICUMSA) 3) HFCS55: 0.00 to 80.00% (ICUMSA conformed) to 100% (out of ICUMSA) HFCS42 shows 42% fructose within isomerized sugar. Concentration of isomerized sugar adopts conversion table of Com Product Inc. Temperature compensation range Minimum sample Measuring time Display Sampling Temperature compensation Statistical computation. External connection 1) Brix : 10 to 40ûC 2) HFCS42/55 : 20 to 60ûC 0.2mL 4 seconds ( depends on sample ) 1) LC display 2) Contents: Brix and Isomerized sugar concentration Manual by dripping pipette Brix and Isomerized sugar concentration Mean value, standard deviation, relative standard deviation. Automatic computation by the built-in processor. 1) Personal computer: RS-232C serial interface 2) External printer: RS-232C serial interface Accuracy Repeatability Resolution 1) Brix : ±0.03% ( 0.00 to 85.00% ) 2) HFCS42 : ±0.03% ( 0.00 to 76.00% ) 3) HFCS55 : ±0.03% ( 0.00 to 80.00% ) ±0.01% 0.01% Ambient condition Power source Power consumption Dimension Weight Temperature: 5 ~ 35ûC Humidity: less than 85%RH (under no condensation) AC100 ~ 240V, 50/60Hz 20W 288(W) 468(D) 247(H)mm 6 kg approx Distributed by Overseas Division : 8-3 Niban-cho Chiyoda-ku TOKYO , JAPAN Fax : , Phone : URL : Specifications and design subject to change for improvements without notice. Printed in Japan. 211TD53C
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