IC Section. Starter Kits IC SPE Standards EZ-LUTE Buffers Other Accessories

Transcription

1 IC Section IC Columns Introduction Allsep Anion Columns Novosep Anion Columns Other Anion Columns Universal Cation Columns Cation & Organic Acid Columns Transition Metal Columns Surfactant Columns Guard Cartridges IC Accessories Starter Kits IC SPE Standards EZ-LUTE Buffers Other Accessories IC Instruments Suppressors Conductivity Detector Column Heater IC Detection Systems

2 I C C O L U M N S IC Columns Introduction Ion Chromatography Alltech has a complete line of products for Ion Chromatography Accessories Keep your system running smoothly with Alltech s IC accessories, including certified standards, sample prep devices, and metal-free tubing and fittings. Columns We manufacture a full line of high quality anion, cation, and specialty IC columns. Instruments Easily add IC detection to your existing HPLC equipment with Alltech s patented Suppressors and our rugged, temperature controlled Conductivity Detector. What is the difference between suppressed and non-suppressed detection? Suppressor-based detection is the best method for anion analysis. Some anion exchange mobile phases are highly conductive, and can interfere with conductivity detection they introduce too much background to visualize the low-level anions in the sample. Mobile phase conductivity needs to be suppressed to efficiently detect sample anions at low concentrations. Suppressors allow the ppb-level sensitivity required for many EPA methods. Suppressors also let you use gradient elution for complex samples. Non-suppressed detection is the best method for cation analysis. It is sometimes called Single Column Ion Chromatography (SCIC). It is also useful for anion exchange applications that do not require ppblevel sensitivity Applications See pages for IC applications. Tips for IC Column Selection Particle Size - For common anion and cation separations, smaller particle size resins will result in better peak shapes and higher resolution than larger particle size resins. Larger particle sizes may offer better flow rates for some sample types. Polymer vs. Silica - Polymer-based columns have higher capacities and broader ph stability. Silica-based columns give sharper peaks and overall better efficiency, but have a limited ph range and are not compatible with suppressor-based methods. Suppressed vs. Non-Suppressed Detection - Most anion separations require suppression, while most cation separations do not. Consider whether a column has been optimized for suppressor-based detection, or is better suited to non-suppressed (single column) detection. Column Length - For the same packing material, shorter columns provide faster run times, while longer columns provide better resolution. Always pick the packing that has the best selectivity for your sample, and then pick the column length based on the tradeoff between resolution and speed, taking into consideration the complexity of your sample and your desired run time. Column i.d. - Narrower columns provide better mass sensitivity, accommodate smaller sample sizes, and significantly reduce solvent usage. Wider columns allow for larger sample sizes and minimize the negative effects of the system s dead volume

3 IC Columns Introduction I C C O L U M N S IC Column Specifications COLUMN COMPOSITION PH RANGE APPLICATIONS Anion Exchange Columns Allsep Allsep A-2 Novosep A-1 Novosep A-2 Anion/S Anion/R PRP -X100 AN1 Cation Exchange Columns Universal Cation Universal Cation HR PRP -X200 Organic Acid Columns PRP -X300 Prevail Organic Acid OA and IOA Anion Exclusion Specialty Columns Transition Metal Surfactant/R Surfactant C8 Surfactant C18 polymer-based anion exchanger, 7µm polymer-based anion exchanger, 7µm polymer-based anion exchanger, 7µm polymer-based anion exchanger, 5µm silica-based anion exchanger, 10µm polymer-based anion exchanger, 10µm polymer-based anion exchanger, 10µm polymer-based anion exchanger, 9µm silica-based cation exchanger, 7µm silica-based cation exchanger, 3µm polymer-based cation exchanger, 10µm polymer-based sulfonated cation exchanger silica-based reversed phase, 3µm and 5µm polymer-based cation exchanger, 6.5µm, 8µm, and 9µm polymer-based cation exchanger, 10µm silica-based reversed phase, 3µm and 7µm polymer-based reversed phase, 7µm silica-based reversed phase, 5µm silica-based reversed phase, 5µm ph 2-10 ph 2-11 ph 2-11 ph 3-12 inorganic anions, weak and strong acid ions, metal complexes, organic acids inorganic anions, organic acids, suitable for both weak and strong anions in a single run inorganic anions, weak and strong acid anions; ideal for separation of hydrophobic anions inorganic anions and oxyhalides; ideal for separation of 7 common anions plus 3 oxyhalide anions in one run EPA METHODS 300.0, Part A 300.0, Part A OPTIMIZED FOR SUPPRESSED OR NON-SUPPRESSED CONDUCTIVITY PAGE both 307 both suppressed , 300.1, 317.0, suppressed 308 ph inorganic anions non-suppressed 309 ph 2-12 ph 1-13 ph 2-13 ph 2-7 ph 2-7 ph 1-13 ph 1-13 inorganic anions, weak and strong acid ions inorganic anions, weak and strong acid ions inorganic anions, weak and strong acid ions, organic acids groups I and II cations, amines, divalent transition metals groups I and II cations, amines, divalent transition metals; smaller particle size for improved peak resolution groups I and II cations (separate runs), amines, lanthanides simple aliphatic carboxylic acids and alcohols non-suppressed 309 non-suppressed 310 both both both 311 non-suppressed 312 non-suppressed 312 ph 1-9 organic acids N/A 313 ph 1-14 organic acids, aromatic acids, sugars/ organic acids/alcohols in one run; multiple particle sizes available non-suppressed 313 ph 1-13 organic acids, weak acid anions non-suppressed 314 ph 2-7 ph 1-14 high capacity, separates divalent transition metals and metal-cyano complexes more selectively than a cation exchange column anionic and cationic surfactants; separate short and long chain surfactants in one gradient run non-suppressed 314 both 315 ph 2-7 short chain anionic surfactants non-suppressed 315 ph 2-7 long chain or aromatic anionic surfactants non-suppressed

4 I C C O L U M N S Anions Alltech Anion Columns Allsep Anion IC Columns Resolves fluoride away from the water dip Meets requirements for U.S. EPA Method part A The Allsep Anion Column is compatible with common IC mobile phases: carbonate, bicarbonate, p-hydroxybenzoic acid, phthalic acid, succinic acid, and sodium octane sulfonate. Use this column for performing EPA Method Part A Determination of Inorganic Anions in Water per U.S. EPA guidelines. Allsep A-2 Anion IC Columns Separates both weak and strong acid anions in one run Resolves formate away from fluoride and chloride The Allsep A-2 Anion Column separates both weakly retained organic and strongly retained inorganic anions in one run. The high capacity Allsep A-2 Anion Column is also suitable for the separation of chlorite and chlorate. Resolves Fluoride Away from the Water Dip Organic and Inorganic Anions 1. Fluoride 2. Chloride 3. Nitrite 4. Bromide 5. Nitrate 6. Phosphate 7. Sulfate Fluoride (1.0ppm) 2. Acetate (1.0ppm) 3. Formate (1.0ppm) 4. Chlorite (1.0ppm) Chloride (2.0ppm) 6. Nitrite (2.0ppm) 7. Bromide (2.0ppm) 8. Chlorate (5.0ppm) 9. Nitrate (2.0ppm) 10. Phosphate (3.0ppm) 11. Sulfate (3.0ppm) Column: Allsep Anion, 7µm, 4.6 x 100mm (Part No ) Mobile Phase: 0.85mM NaHCO3/0.9mM Na2CO3 Flow Rate: 1.2mL/min Detector: Suppressed Conductivity Column: Allsep A-2 Anion, 7µm, 4.6 x 100mm (Part No ) Mobile Phase: 2.1mM NaHCO3/1.6mM Na2CO3 Flow Rate: 2.0mL/min Detector: Suppressed Conductivity Allsep Anion Specifications Composition: Methacrylate based w/quaternary ammonium functional groups Particle Size: 7µm Mobile Phase Limits: ph 2-10, 0-100% organic modifier Allsep A-2 Anion Specifications Composition: Methacrylate based w/quaternary alkanol amine functional groups Particle Size: 7µm Mobile Phase Limits: ph 2-11, 0-100% organic modifier ALLSEP ANION COLUMNS ALLSEP A-2 ANION COLUMNS PACKING I.D. X LENGTH PEEK SS Allsep Anion 4.6mm x mm x mm x mm x PACKING I.D. X LENGTH PEEK SS Allsep A-2 4.6mm x mm x Need an All-Guard holder? Save money with IC All-Guard Kits on page 316. GUARDS FOR ALLSEP AND ALLSEP A-2 COLUMNS DESCRIPTION QTY. GA-1 Anion Cartridges All-Guard Cartridge Holder* ea *Direct-Connect Column Coupler included. 307

5 Anions I C C O L U M N S Alltech Anion Columns Novosep A-1 Anion IC Columns Separates common anions and strongly retained hydrophobic anions Meets the requirements of U.S. EPA Method The Novosep A-1 Column is your best choice for routine anion analysis and for determining other strongly retained hydrophobic anions that previously required specialty columns. The Novosep A-1 Column can simultaneously separate weakly and strongly retained anions by gradient elution. Carbonate/bicarbonate gradients offer a powerful tool for separating anions that have a wide range of affinities for the column. Novosep A-2 Anion IC Columns Separates seven inorganic anions and three oxyhalide anions in a single run Meets the requirements of U.S. EPA Methods 300.0, 300.1, 317.0, and The Novosep A-2 Anion Column is ideal for the separation of 10 inorganic anions by suppressor-based ion chromatography using U.S. EPA Method The column can also be used to determine inorganic anions in drinking water per U.S. EPA Method and trace bromate in drinking water using U.S. EPA Methods and Weakly and Strongly Retained Anions 9299 Anions in High Ionic Strength Water by EPA Method Acetate (5ppm) 2. Chloride (1ppm) Min. Column: Novosep A-1, 7µm, 4.6 x 100mm (Part No ) Mobile Phase: A: Water B: 10mM Sodium Bicarbonate C: 10mM Sodium Carbonate with 1mM Cyanophenol Gradient: Time: %B: %C: Flow Rate: 1.5mL/min Detector: Suppressed Conductivity 7 3. Phosphate (5ppm) 4. Sulfate (1ppm) 5. Oxalate (5ppm) 6. Perchlorate (5ppm) 7. Citrate (5ppm) 1. Fluoride 2. Chlorite Column: Novosep A-2 Anion, 5µm, 4.0 x 250mm (Part No ) Mobile Phase: 3.6mM NaHCO3 Flow Rate: 0.8mL/min Detector: Suppressed Conductivity 3. Bromate 4. Chloride 5. Nitrite-N 6. Bromide 7. Chlorate 8. Dichloroacetate 9. Nitrate-N 10. Phosphate-P 11. Sulfate Novosep A-1 Anion Specifications Composition: Methacrylate based w/quaternary ammonium functional groups Particle Size: 7µm Mobile Phase Limits: ph 2-11, 0-100% organic modifier (methanol, acetonitrile, acetone) Novosep A-2 Anion Specifications Composition: Polyvinyl alcohol based w/quaternary ammonium functional groups Particle Size: 5µm Mobile Phase Limits: ph 3-12, 0-100% organic modifier, 10% modifier is practical upper limit PACKING NOVOSEP A-1 ANION COLUMNS I.D. X LENGTH PEEK SS Novosep A-1 4.6mm x mm x mm x mm x PACKING NOVOSEP A-2 ANION COLUMN I.D. X LENGTH PEEK Novosep A-2 4.0mm x GUARDS FOR NOVOSEP AND NOVOSEP A-2 ANION COLUMNS DESCRIPTION QTY. GA-1 Anion Cartridges All-Guard Cartridge Holder* ea *Direct-Connect Column Coupler included. 308

6 I C C O L U M N S Anions Alltech Anion Columns Anion/S IC Columns Silica-based for symmetrical peak shapes Separates inorganic and organic anions The Anion/S Columns are best suited for routine separations of chloride, bromide, nitrate, and sulfate. Not suitable for fluoride analysis. EDTA and Other Anions 8284 Anion/R IC Columns Polymer-based for broad ph stability Separates seven common inorganic anions Because of their high ph stability, these column are ideal for the separation of anions using high ph mobile phases such as p-hydroxybenzoate, sodium hydroxide, and sodium carbonate/bicarbonate. The larger dimension columns (150mm and 250mm) are suitable for a high resolution separation of the seven common inorganic anions. Weak Acid Anions Nitrite 2. EDTA 3. Nitrate 4. Bromide 1. Borate (5ppm) 2. Silicate (10ppm) 3. Formate (10ppm) 4. Sulfide (10ppm) 5. Chloride (20ppm) 6. Cyanide (20ppm) Column: Anion/S, 10µm, 4.6 x 100mm (Part No ) Mobile Phase: 1.5mM Sulfuric Acid Flow Rate: 1.0mL/min Detector: UV at 210nm Column: Anion/R, 10µm, 4.1 x 250mm (Part No ) Mobile Phase: 4mM Sodium Hydroxide/ 0.1mM Sodium Benzoate Flow Rate: 1.5mL/min Detector: Conductivity ANION/S SPECIFICATIONS Composition: Silica w/quaternary ammonium ion exchanger Particle Size: 10µm Exchange Capacity: 0.25meq/g Mobile Phase Limits: ph 2-5.5, 0-100% organic modifier ANION/R SPECIFICATIONS Composition: Poly(styrene-divinylbenzene) Trimethylammonium Particle Size: 10µm Exchange Capacity: 0.19 ± 0.02meq/g Mobile Phase Limits: ph 2-12, 0-100% organic modifier ANION/S COLUMNS ANION/R COLUMNS PACKING I.D. X LENGTH PEEK SS Anion/S 4.6mm x mm x PACKING I.D. X LENGTH PEEK SS Anion/R 4.1mm x mm x mm x mm x Need an All-Guard holder? Save money with IC All-Guard Kits on page 316. GUARDS FOR ANION/S AND ANION/R COLUMNS DESCRIPTION QTY. GA-1 Anion Cartridges All-Guard Cartridge Holder* ea *Direct-Connect Column Coupler included. 309

7 Anions I C C O L U M N S Anion Columns IC Sep AN1 IC Column Separates anions with carbonate/bicarbonate and hydroxide mobile phases The IC Sep AN1 Column is compatible with both suppressor-based and non-suppressed detection. A variety of mobile phases including sodium carbonate/ bicarbonate, sodium hydroxide, and borate/gluconate may be used. Separation of Sulfite and Sulfate Hamilton PRP -X100 IC Columns High ph stability makes this column ideal for the separation of weak anions. PRP -X100 provides excellent resolution for the seven most frequently analyzed inorganic anions. For non-suppressed analyses only. EDTA in Eye Drops 1. Fluoride 2. Chloride 3. Nitrite 4. Bromide 5. Nitrate 6. Phosphate 7. Sulfite 8. Sulfate CuEDTA 8068 Column: IC Sep AN1, 9µm, 4.6 x 250mm (Part No ) Mobile Phase: 1.8mM NaHCO3, 1.8mM Na2CO3 Flow Rate: 1.0mL/min Detector: Suppressed Conductivity Column: PRP -X100, 10µm, 4.1 x 150mm (Part No ) Mobile Phase: 0.003N Sulfuric Acid Flow Rate: 2mL/min Detector: UV at 254nm IC Sep AN1 Specifications Composition: Poly(styrene-divinylbenzene) alkyl dimethyl ethanol ammonium functional group Particle Size: 9µm Exchange Capacity: 0.05meq/g Mobile Phase Limits: ph 2-13, No organic solvents IC SEP AN1 COLUMNS PACKING I.D. X LENGTH AN1 4.6mm x Choose a guard column or integral guard discs to protect your IC Sep Column. Hamilton PRP -X100 Specifications Composition: Trimethylammonium psdvb copolymer Particle Size: 10µm, spherical Exchange Capacity: 0.19meq/g Mobile Phase Limits: ph 1-13, 0-100% organic modifier HAMILTON PRP -X100 PACKING I.D. X LENGTH PRP X mm x mm x mm x mm x mm x IC SEP GUARDS DESCRIPTION QTY Guard Column, 50 x 4.6mm ea Guard Column Guard-Disc HAMILTON GUARD KITS AND CARTRIDGES DESCRIPTION QTY Guard Column Starter Kits PRP -X100, 1 Holder, 2 Cartridges ea Replacement Guard Cartridges(Requires Hamilton Guard Holder) PRP -X100, 25 x 2.3mm

8 I C C O L U M N S Cations Alltech Cation Columns Universal Cation and Universal Cation HR IC Columns Separate Groups I and II cations in one isocratic run Separate transition metals without post-column reaction New Universal Cation HR 3µm particle size for improved efficiency Compatible with a variety of mobile phases including complexing acids (citric acid, tartaric acid, oxalic acid), mineral acids (nitric acid, hydrochloric acid, sulfuric acid) and non-complexing organic acids (methanesulfonic acid). 1. Lithium (0.12ppm) 2. Sodium (0.90ppm) 3. Ammonium (0.90ppm) Cesium and Strontium Potassium (1.5ppm) 5. Cesium (0.8ppm) 6. Magnesium (1.2ppm) 7. Calcium (1.2ppm) 8. Strontium (1.6ppm) Metal Ions 1. Lithium (0.2ppm) 2. Sodium (1.5ppm) 3. Ammonium (1.5ppm) 4. Potassium (2.5ppm) Nickel (2.5ppm) 6. Zinc (2.5ppm) 7. Cobalt (2.5ppm) 8. Magnesium (2.0ppm) 9. Calcium (2.0ppm) Column: Universal Cation, 7µm, 4.6 x 100mm (Part No ) Mobile Phase: 3mM Methanesulfonic Acid Flow Rate: 1.0mL/min Detector: Conductivity Rapid Analysis of Monovalent and Divalent Cations Column: Universal Cation, 7µm, 4.6 x 100mm (Part No ) Mobile Phase: 2mM Tartaric Acid/1mM Oxalic Acid Flow Rate: 1.0mL/min Detector: Conductivity Lithium 2. Sodium 3. Ammonium 4. Potassium 5. Magnesium 6. Calcium Universal Cation Specifications Composition: Silica coated with polybutadiene/maleic acid copolymer Particle Size: 3µm and 7µm, spherical Mobile Phase Limits: ph 2-7, 0-100% organic modifier Min. Column: Universal Cation HR, 3µm, 7.0 x 53mm (Part No ) Mobile Phase: 3mM Methanesulfonic Acid Flow Rate: 2.5mL/min Detector: Conductivity PACKING UNIVERSAL CATION COLUMNS PEEK I.D. X LENGTH SS 7µm 4.6mm x 100mm µm HR 4.6 x 100mm x 53mm GUARDS FOR UNIVERSAL CATION COLUMNS DESCRIPTION QTY. GC-2 Universal Cation GC-3 Universal Cation HR All-Guard Cartridge Holder* ea *Direct-Connect Column Coupler Included. Need an All-Guard holder? Save money with IC All-Guard Kits on page

9 Cations and Organic Acids I C C O L U M N S Hamilton Cation & Organic Acid Columns Hamilton PRP -X200 Cation Analysis Columns For separation of the alkali metal and alkaline earth cations in separate runs. Hamilton PRP -X300 Organic Acid Columns For separation of simple aliphatic carboxylic acids and alcohols. Inorganic anions are not retained. Alkali Metals Endothall Lithium 2. Sodium 3. Ammonium 4. Potassium 5. Cesium 1. Maleic Acid (1160ppm) 2. Malonic Acid (2000ppm) 3. Succinic Acid (210ppm) 4. Fumaric Acid (12.3ppm) 5. Endothall (9080ppm) Column: PRP -X200, 10µm, 4.1 x 150mm (Part No ) Mobile Phase: 0.004N HNO3 in 30% MeOH Flow Rate: 2mL/min Detector: Conductivity Column: PRP -X300, 7µm, 4.1 x 150mm Mobile Phase: 1.5mN Sulfuric Acid: Acetonitrile (92:8) Flow Rate: 0.5mL/min Detector: UV at 210nm Hamilton PRP -X200 Specifications Composition: Sulfonated psdvb copolymer Particle Size: 10µm, spherical Exchange Capacity: 35 umeq/g Mobile Phase Limits: ph 1-13, 0-100% organic modifier Hamilton PRP -X300 Specifications Composition: Sulfonated psdvb in H+ form Particle Size: 3µm and 7µm Mobile Phase Limits: ph 1-13, 0-100% organic modifier HAMILTON PRP -X200 COLUMNS PARTICLE SIZE I.D. X LENGTH PRP -X x 150mm x 250mm x 150mm x 250mm HAMILTON PRP -X300 COLUMNS PARTICLE SIZE I.D. X LENGTH PRP -X300, 3um 4.1 x 150mm PRP -X300, 7um 4.1 x 150mm x 250mm x 150mm HAMILTON PRP -X200 GUARD KITS AND CARTRIDGES DESCRIPTION QTY Guard Column Starter Kit PRP -X200, 1 Holder, 2 Cartridges Replacement Guard Cartridges (Requires Hamilton Guard Holder) PRP -X200, 25 x 2.3mm ea HAMILTON PRP -X300 GUARD KITS AND CARTRIDGES DESCRIPTION QTY Guard Column Starter Kit PRP -X300, 1 Holder, 2 Cartridges Replacement Guard Cartridges (Requires Hamilton Guard Holder) PRP -X300, 25 x 2.3mm ea

10 I C C O L U M N S Organic Acids Alltech Organic Acid Columns Alltech OA and IOA Analyze organic acids and alcohols in a single run Polymer-based for broad ph stability OA: selective for aliphatic and aromatic acids Use OA-1000 for inorganic anions and most organic acids. Use OA-2000 for low pka organic acids, low molecular weight straight chain acids, and aromatic acids. IOA: separate short chain organic acids from fructose and glucose Use IOA-1000 for TCA or Krebs cycle acids, carbohydrates, and alcohols. Use IOA-2000 for fast separation of acids and some alcohols. Prevail OA Modified silica for higher flow rates and broader ph stability Acid-stable for long column lifetimes Does not require a column heater Prevail OA (Organic Acid) Columns separate organic acids with high efficiency, sharp peaks, and short run times. Use ph adjustments to adjust the selectivity for di-and tri-carboxylic acids. Wine Analysis by High Resolution Ion Exclusion Column: IOA-1000, 9µm, 7.8 x 300mm (Part No. 9646) Mobile Phase: 0.005N Sulfuric Acid Flow Rate: 0.3mL/min Column Temp: 60 C Detector: RI 1. Citric Acid 2. Tartaric Acid 3. Glucose 4. Malic Acid 5. Fructose 6. Acetic Acid 7. Glycerol 8. Lactic Acid 9. Methanol 10. Ethanol 5411 Organic Acids Column: Prevail Organic Acid, 5µm, 4.6 x 150mm (Part No ) Mobile Phase: 25mM KH2PO4, ph2.5 Flow Rate: 1.5mL/min Detector: UV at 210nm Oxalic Acid 5. Lactic Acid 2. Tartaric Acid 6. Acetic Acid 3. Formic Acid 7. Diglycollic Acid 4. Malic Acid 8. Maleic Acid 9. Citric Acid 10. Succinic Acid 11. Fumaric Acid OA and IOA Specifications Composition: Highly sulfonated poly (styrenedivinylbenzene) cation exchanger Particle Size: 6.5µm, 8µm, and 9µm Mobile Phase Limits: ph 1-14, isocratic aqueous mobile phases only; no organics Prevail OA Specifications Composition: Silica base, with specially modified reversed-phase functional groups Particle Size: 3µm and 5µm Mobile Phase Limits: ph 1-9, 0-100% organic modifier ALLTECH ORGANIC ACID COLUMNS PACKING I.D. X LENGTH OA-1000, 9µm 6.5 x 300mm 9046 OA-2000, 6.5µm 6.5 x 100mm 9048 IOA-1000, 9µm 7.8 x 300mm 9646 IOA-2000, 8µm 6.5 x 150mm 9648 PREVAIL ORGANIC ACID COLUMNS PACKING I.D. X LENGTH Prevail OA, 3µm 4.6 x 100mm x 150mm Prevail OA, 5µm 4.6 x 150mm x 250mm GUARDS FOR OA AND IOA COLUMNS DESCRIPTION QTY. Organic Acid Guard Cartridge Kit Includes: 1 Holder, 2 Cartridges ea Organic Acid Cartridge, 24 x 4.0mm PREVAIL ORGANIC ACID ALL-GUARD CARTRIDGES DESCRIPTION QTY. Prevail OA (holder required) All-Guard Cartridge Holder* ea *Direct-Connect Column Coupler included. 313

11 Transition Metals I C C O L U M N S Anion Exclusion Alltech Anion Exclusion Columns Separate organic acids and weak acid anions Polymer-based for broad ph stability Economical choice for small inorganics Anion Exclusion Columns separate organic acids and weakly ionized anions by an anion exclusion mechanism. Dilute mineral acids are the typical mobile phases for the separation. Acetonitrile may be used as an organic modifier to decrease the retention of hydrophobic compounds. Transition Metal Alltech Transition Metal Columns Separate divalent transition metals and metal-cyano complexes more selectively than a cation exchange column To separate divalent transition metals, use conductivity detection for concentrations as low as 1ppm, or UV detection with post-column reaction for ppb-level concentrations To separate metal-cyano complexes, use Alltech s low UV IPC A (Part No ) as the ion-pair reagent, and methanol or acetonitrile as the organic modifier. Organic Acid Standards Metal-Cyano Complexes Oxalic Acid 2. Maleic Acid 3. Malic Acid 4. Succinic Acid 5. Formic Acid 6. Acetic Acid Min. 1. Fe(CN) Ag(CN)2-3. Fe(CN) Ni(CN) Au(CN)2 - Column: Anion Exclusion, 10µm, 300 x 7.5mm (Part No ) Mobile Phase: 1.5mM Sulfuric Acid Flow rate: 0.4mL/min Detector: Conductivity Anion Exclusion Specifications Composition: Highly sulfonated poly (styrenedivinylbenzene) cation exchanger Particle Size: 10µm Mobile Phase Limits: <10% acetonitrile, no methanol <5% isopropyl or ethyl alcohols Column: Transition Metal C18, 7µm, 250 x 4.6mm (Part No ) Mobile Phase: 0.005M Tetrabutylammonium Sulfate (Preformulated Alltech Low UV IPC A, Part No ) in 30% Acetonitrile Flow rate: 0.7mL/min Detector: UV at 214nm Transition Metal Specifications Composition: Silica-based with C18 bonded phase (20% Carbon) Particle Size: 3µm and 7µm, spherical Mobile Phase Limits: ph 2-7 PACKING ANION EXCLUSION COLUMNS PEEK I.D. X LENGTH SS Anion Exclusion, 10µm 7.5 x 100mm x 300mm x 100mm x 300mm TRANSITION METAL COLUMNS PACKING I.D. X LENGTH PEEK C18, 3µm 4.6 x 100mm C18, 7µm 4.6 x 250mm ANION EXCLUSION ALL-GUARD CARTRIDGES DESCRIPTION QTY. Anion Exclusion GIE All-Guard Cartridge Holder* ea *Direct-Connect Column Coupler included. TRANSITION METAL ALL-GUARD CARTRIDGES DESCRIPTION QTY. GRP-2 Revised Phase All-Guard Cartridge Holder* ea *Direct-Connect Column Coupler included. 314 Need an All-Guard holder? Save money with IC All-Guard Kits on page 316.

12 I C C O L U M N S Surfactants Alltech Surfactant Columns Surfactant/R IC Columns Separate anionic and cationic surfactants Separate short and long chain surfactants in one gradient run The Surfactant/R Column separates anionic and cationic surfactants by ion-pair chromatography along with suppressed conductivity detection. This column can also be used for other reversed-phase applications. Surfactant/C IC Columns For non-suppressed analyses Silica-based for symmetrical peak shapes Choose Surfactant C8 for short chain anionic surfactants such as alkyl sulfates and alkyl sulfonates. Choose Surfactant C18 for long chain or aromatic surfactants such as xylene sulfonate and dodecyl benzene sulfonate. Short and Long Chain Cationic Surfactants Alkyl Sulfates 1. Tetramethyl Ammonium (20ppm) 2. Tetraethyl Ammonium (20ppm) 3. Tetrapropyl Ammonium (20ppm) 4. Tetrabutyl Ammonium (30ppm) 5. Dodecyltrimethyl Ammonium (30ppm) 6. Cetyltrimethyl Ammonium (40ppm) 7. Myristyltrimethyl Ammonium (50ppm) Octyl Sulfate 2. Decyl Sulfate 3. Dodecyl Sulfate 5998 Column: Surfactant/R Column, 7µm, 4.6 x 150mm (Part No ) Mobile Phase: A: 2mM Nonafluoropentanoic Acid B: 100% Acetonitrile Gradient: Time: %B: Flow rate: 1.0mL/min Detector: Suppressed Conductivity Column: Mobile Phase: Flow rate: Detector: Surfactant C8, 5µm, 4.6 x 250mm (Part No ) 0.02g/L Ammonium Acetate in 40% Methanol 1.2mL/min Conductivity Surfactant/R Specifications Composition: Polydivinylbenzene (DVB) based resin Particle Size: 7µm Mobile Phase Limits: ph 1-14, 0-100% organic modifier Surfactant/C Specifications Composition: Silica-based C8 or C18 Particle Size: 5µm Mobile Phase Limits: ph 2-7, 0-100% organic modifier PACKING I.D. X LENGTH SURFACTANT/R COLUMNS PEEK SS Surfactant/R, 7µm 4.6 x 150mm PACKING SURFACTANT/C COLUMNS PEEK I.D. X LENGTH SS C8, 5µm 4.6 x 250mm C18, 5µm 4.6 x 250mm GUARDS FOR SURFACTANT/R COLUMNS DESCRIPTION QTY. GRP-1 Revised Phase All-Guard Cartridge Holder* ea *Direct-Connect Column Coupler included. GUARDS FOR SURFACTANT/C COLUMNS DESCRIPTION QTY. GRP-2 Revised Phase All-Guard Cartridge Holder* ea *Direct-Connect Column Coupler included. Need an All-Guard holder? Save money with IC All-Guard Kits on page

13 Guard Cartridges I C C O L U M N S IC Guard Cartridges Convenient - change cartridges in seconds without tools No connecting tubing required All-Guard Holders work with other manufacturer s columns Inert, metal-free PEEK fluid path Protect your IC columns from contaminants with All-Guard Cartridges. The All-Guard System s unique holder is open on the side for fast and easy installation of cartridges. Save money with IC Guard Cartridge Starter Kits, including an All-Guard Holder and three guard cartridges. Connect the All-Guard Holder directly to your analytical column using Alltech s patented Direct- Connect Column Coupler, included with each holder Guard Reference Table COMPANY COLUMN TYPE CARTRIDGE TYPE ALL-GUARD KIT Alltech Allsep, Allsep A-2 GA Novosep A-1 GA Novosep A-2 GA Anion/R, Anion/S GA Universal Cation GC Universal Cation HR GC Anion Exclusion GIE Transition Metal C18 GRP Surfactant/R GRP Surfactant C8, C18 GRP Dionex IonPac AS4, AS4A, AS4A-SC, GA AS7, AS9, AS10, AS11, AS12, AS14 IonPac CS3, CS10, CS11 GC IonPac CS12, CS14, CS15 GC IonPac NS1 GRP IonPac AS1, AS5 GIE Waters IC-Pak -A GA IC-Pak -M/D GC Ion Exclusion GIE µbondapak C18 GRP Radial-Pak C18 GRP Nova-Pak C18 GRP Hamilton PRP -X100 GA PRP -X200 GC PRP -X300 GIE PRP -1 GRP Misc. Sarasep ANIONSEP AN1 GA Bio-Rad, Aminex, HPX-87 GIE Interaction ION-120 GA Interaction ION-210 GC Interaction ORH-801 GIE Metrohm Metrosep GA Metrohm SUPER SEP GC ALL-GUARD KITS DESCRIPTION QTY. All-Guard Kits (Includes 3 PEEK Cartridges, 1 Holder, and 1 Direct-Connect Column Coupler) GA-1 Anion ea GC-1 Cation ea GC-2 Universal Cation ea GC-3 Universal Cation HR ea GIE-1 Anion Exclusion ea GRP-1 Resin Reversed-Phase ea GRP-2 Silica Reversed-Phase ea Guard Holder (Includes Direct-Connect Column Coupler) All-Guard Cartridge Holder ea Replacement Column Coupler ea REPLACEMENT CARTRIDGES DESCRIPTION QTY. All-Guard Replacement Cartridges, PEEK GA-1 Anion GC-1 Cation GC-2 Universal Cation GC-3 Universal Cation HR GIE-1 Anion Exclusion GRP-1 Resin Reversed-Phase GRP-2 Silica Reversed-Phase

14 I C A C C E S S O R I E S Starter Kits IC Starter Kits Alltech offers several IC kits to help you set up and maintain your IC system quickly and economically Non-suppressed IC Anion Kit For the analysis of anions by non-suppressed IC methods NON-SUPPRESSED IC ANION KIT DESCRIPTION Non-suppressed IC Anion Kit Includes: Part No : Allsep Anion Column, 4.6 x 100mm Part No : All-Guard GA-1 Guard Cartridge Kit Part No : 12 x 25mL EZ-LUTE 4mM phba Buffer Concentrates Part No : Anion Standards Kit U.S. EPA Methods Anion Kit U.S. EPA Methods 300.0, 300.1, 317.0, and Mono- and Divalent Cation Kit For the simultaneous analysis of monovalent and divalent cations using the Universal Cation Column U.S. EPA METHODS ANION KIT DESCRIPTION U.S. EPA Methods Anion Kit Includes: Part No : Novosep A-2 Anion Column, 4.0 x 250mm Part No : All-Guard GA-1 Guard Cartridge Kit Part No : 100mL EZ-LUTE 3.6mM Carbonate Buffer Concentrate Part No : Anion and Oxyhalide Standards Kit MONO- AND DIVALENT CATION KIT DESCRIPTION Mono- and Divalent Cation Kit Includes: Part No : Universal Cation Column, 4.6 x 100mm Part No : All-Guard GC-2 Guard Cartridge Kit Part No : 12 x 25mL EZ-LUTE 3mM Methanesulfonic Acid Buffer Concentrates Part No : Cation Standards Kit Suppressor-based IC Anion Kit For the analysis of anions by suppressor-based IC methods SUPPRESSOR-BASED IC ANION KIT DESCRIPTION Suppressor-based IC Anion Kit Includes: Part No : Novosep A-1 Anion Column, 4.6 x 150mm Part No : All-Guard GA-1 Guard Cartridge Kit Part No : 100mL EZ-LUTE 1.7mM Bicarbonate/1.8mM Carbonate Buffer Concentrate Part No : Anion Standards Kit To Purchase Kit Components Separately: Guard Cartridges...page 316 Allsep Columns...page 307 Novosep Columns...page 308 Universal Cation Columns...page 311 Standards...page 319 EZ-LUTE Buffers...page 320 IC Accessory Kit A must for new and experienced IC users IC ACCESSORY KIT DESCRIPTION IC Accessory Kit Includes: Part No : 1/16" x 0.007" i.d. PEEK Tubing, 10ft Part No : 1/16" x 0.010" i.d. PEEK Tubing, 10ft Part No : One-Piece Fitting, 10/pk Part No : PEEK 1/16" Union Part No : #22Ga Needle with CTFE Luer-Hub, 6/pk Part No : 1mL Plastic Syringe for Injection, 12/pk Part No : 20mL Plastic Syringe for Priming Pump, 2/pk Part No. 3206: Plastic Tubing Cutter Part No. 1998: Open-End Wrench Part No : Metal-Free Solvent-Inlet Filter Part No : Metal-Free In-Line Filter 317

15 SPE I C A C C E S S O R I E S Maxi-Clean IC Cartridges Improve your chromatography seven resin types remove interfering ions from your sample Protect your investment remove metals, salts, and surfactants that can irreversibly damage expensive IC columns and instruments Metal-free construction Unextracted IC-Ag Removes Chloride Nitrate in Brine 1. Fluoride 2. Nitrate 3. Sulfate Extracted Min Min. Maxi-Clean IC Cartridges are easy to process by syringe 6225 Column: Novosep A-1, 7µm, 4.6 x 150mm (Part No ) Mobile Phase: 1.7mM NaHCO3, 1.8mM Na2CO3 Flow Rate: 1.2mL/min Detector: Suppressed Conductivity IC-Ag removes high levels of chloride, revealing the other ions in the sample. MAXI-CLEAN IC CARTRIDGES RESIN TYPE REMOVES 0.5mL Bed Volume, 50/pk IC-RP Hydrophobic components IC-OH Anions; increases ph IC-H Cations; decreases ph IC-Ag Chloride, iodide, bromide IC-Ba Sulfate IC-Na Cations IC-Chelate Transition metals mL Bed Volume, 25/pk IC-RP Hydrophobic components IC-OH Anions; increases ph IC-H Cations; decreases ph IC-Ag Chloride, iodide, bromide IC-Ba Sulfate IC-Na Cations IC-Chelate Transition metals IC-Chelate Protects Anion Columns Unextracted Tap Water Sodium 2. Potassium 3. Magnesium 4. Calcium 1 Extracted Min. Column: Universal Cation, 7µm, 4.6 x 100mm (Part No ) Mobile Phase: 3mM Methanesulfonic Acid Flow Rate: 1.0mL/min Detector: Suppressed Conductivity IC-Chelate protects anion columns by removing polyvalent metals.

16 I C A C C E S S O R I E S Standards The Largest Selection of IC Standards High purity for sensitive applications Prepared from NIST standard reference materials Certified by two independent methods Certified Organic Anion Standards ORGANIC ANION STANDARDS (125ML) A detailed Certificate of Analysis included 3493 DESCRIPTION 1000PPM Acetate Citrate Formate Glycolate Lactate Malate Maleate Methanesulfonate Nitrilotriacetate (NTA) Oxalate Propionate Succinate Tartrate Certified Anion and Cation Standards STANDARD ANION STANDARDS (125ML) 1000PPM 200PPM Bromate Bromide Chlorate Chloride Chlorite Chromate Fluoride Iodide Nitrate Nitrate-N Nitrite Nitrite-N Perchlorate Phosphate Phosphate-P Sulfate Thiocyanate STANDARD CATION STANDARDS (125ML) 1000PPM 200PPM Ammonium Ammonium-N Calcium Lithium Magnesium Potassium Sodium Certified Organic Acid Standards DESCRIPTION ORGANIC ACID STANDARDS (125ML) 1000PPM Oxalic Acid Maleic Acid Malic Acid Succinic Acid Formic Acid Acetic Acid Citric Acid Tartaric Acid Lactic Acid Abietic Acid Methanesulfonic Acid Certified Amine Standards DESCRIPTION AMINE STANDARDS (125ML) 1000PPM Ethanolamine Diethanolamine Triethanolamine Monomethylamine Dimethylamine Trimethylamine

17 Standards and Buffers I C A C C E S S O R I E S IC Standards Certified Multi-Standard Kits Cost-effective ways to purchase multiple single-ion standards MULTI-STANDARD KITS DESCRIPTION Anion Kits 200ppm Kit: Contains 125mL ea of 200ppm Certified Cl, F, Br, NO3, NO2, PO4, & SO4 1000ppm Kit: Contains 125mL ea of 1000ppm Certified Cl, F, Br, NO3, NO2, PO4, & SO4 Anion and Oxyhalide Kit: (EPA 300.1Part B) Contains 125mL ea of 200ppm certified Cl, F, Br, NO3, NO2, PO4, & SO4, ClO2, ClO3, BrO3 Cation Kits 200ppm Kit: Contains 125mL ea of 200ppm Certified Li, Na, NH4, K, Mg, & Ca 1000ppm Kit: Contains 125mL ea of 1000ppm Certified Li, Na, NH4, K, Mg, & Ca EZ-LUTE Buffer Concentrates Simplify mobile phase preparation For anion, cation, and organic acid analyses Dilution instructions included Certified Multi-Component Mixtures Multiple ions in a single mixture EZ-LUTE Buffers for accurate concentrations every time 6561 MULTI-COMPONENT MIXTURES (125ML) DESCRIPTION Anion Mixtures Mix A: 125mL Mixture Contains: F(10ppm), Cl(20ppm), O2(20ppm), Br(20ppm), NO3(20ppm), PO4(30ppm), SO4(30ppm) Mix 1: 125mL Mixture Contains: F(1ppm), Cl(5ppm), NO2(5ppm), Br(5ppm), NO3(5ppm), PO4(5ppm), SO4(5ppm) Mix 2: 125mL Mixture Contains: F(1ppm), Cl(10ppm), NO2(10ppm), Br(10ppm), NO3(10ppm), PO4 (10ppm), SO4(10ppm) Mix 3: 125mL Mixture Contains: F(10ppm), Cl(20ppm), NO2(20ppm), Br(20ppm), NO3(20ppm), PO4(20ppm), SO4(20ppm) Mix 4: 125mL Mixture Contains: F(20ppm), Cl(40ppm), NO2(40ppm), Br(40ppm), NO3(40ppm), PO4(40ppm), SO4(40ppm) Mix 5: 125mL Mixture Contains: F(25ppm), Cl(50ppm), NO2(50ppm), Br(50ppm), NO3(50ppm), PO4(50ppm), SO4(50ppm) Mix 6: 125mL Mixture Contains: Cl(1000ppm), Br(1000ppm), NO3(1000ppm), PO4(1000ppm), SO4(1000ppm) Mix 7: 125mL Mixture Contains: Cl(15ppm), Br(15ppm), NO3(15ppm), PO4(15ppm), SO4(5ppm) Mix 8: 125mL Mixture Contains: F(25ppm), Cl(50ppm), SO4(100ppm) Cation Mixtures Mix A: 125mL Mixture Contains: Li(0.5ppm), Na(3ppm), NH4(3ppm), K(6ppm) Mix B: 125mL Mixture Contains: Li(0.2ppm), Na(1.5ppm),NH4(1.5ppm), K(2.5ppm), Mg(2.0ppm), Ca(2.0ppm) EZ-LUTE BUFFER CONCENTRATES* DESCRIPTION Non-suppressed Buffers, 12 x 25mL 4mM Phthalic Acid mM Phthalic Acid, ph mM p-hydroxybenzoic Acid mM p-hydroxybenzoic Acid mM p-hydroxybenzoic Acid mM LiOH/Benzoate mM Methanesulfonic Acid Suppressor-based Buffers, 1 x 100mL 1.7mM Bicarbonate/1.8mM Carbonate mM Bicarbonate/2.2mM Carbonate mM Bicarbonate/0.72mM Carbonate mM Bicarbonate/1.6mM Carbonate mM Bicarbonate/1.08mM Carbonate mM Bicarbonate/0.9mM Carbonate mM Bicarbonate/1.2mM Carbonate mM Carbonate mM Bicarbonate mM Carbonate *Concentration after dilution. Custom Standards Available If you need it, we can make it, whether a single ion or a complex mixture. Request a quote for part number C2690, in any volume from 125mL to 1-liter. Fast delivery! 320

18 I C A C C E S S O R I E S IC Accessories PEEK Tubing High-pressure, color-coded, inert COLOR DIMENSIONS O.D. X I.D COLOR-CODED PEEK TUBING PRES. RATE (PSIG) QTY. STRIPED SOLID Red 1/16" x 0.005" 7,000 10ft Yellow 1/16" x 0.007" 7,000 10ft Blue 1/16" x 0.010" 5,000 10ft Orange 1/16" x 0.020" 4,000 10ft Green 1/16" x 0.030" 4,000 10ft For our full selection of PEEK tubing, see page Metal-Free Mobile Phase Inlet Filters Easy connection to fittings METAL-FREE INLET FILTERS DESCRIPTION POROSITY QTY. for 1/8" o.d. 20µm ea for 1/16" o.d. 20µm ea Replacement Polyethylene Filter Elements 20µm For our full selection of Mobile Phase Filters, see pages Polypropylene Vials Metal-free vials for IC samples 4334 Plastic Syringes Economical, disposable polypropylene syringes 4675 POLYPROPYLENE VIALS AND CAPS DESCRIPTION 12x32mm Standard Mouth Screw Thread Vials, 100/pk 100µL Polypropylene Vial with 8/425 Thread /425 Black Cap with TFE/Silicone Liner x32mm Wide Mouth Screw Thread Vials, 100/pk 750µL Polypropylene Vial with 10/425 Thread µL Polypropylene Vial with 10/425 Thread /425 Black Cap with TFE/Butyl Liner /425 Black Cap with TFE/Silicone Liner x32mm Crimp Style Vials, Use 11mm Seal, 100/pk 750µL Polypropylene Vial µL Polypropylene Vial µL Polypropylene Vial µL Polypropylene Vial µL Polypropylene Vial mm Aluminum Seal with TFE/Butyl Liner mm Aluminum Seal with TFE/Silicone Liner mm Poly Crimp Seal with TFE/Butyl Liner mm Poly Crimp Seal with TFE/Silicone Liner DISPOSABLE PLASTIC SYRINGES DESCRIPTION QTY. 1mL Plastic Syringe mL Plastic Syringe mL Plastic Syringe CTFE Luer-Hub Needle for Rheodyne, Valco (VISF-2), and other common injection valves For our full selection of Syringes, see page For our full selection of Vials, see pages SofGrip Fittings High-pressure, ergonomic fittings are stronger than PEEK and handtighten to 5000 psig. For our full selection of SofGrip fittings, see page

19 Suppressors I N S T R U M E N T S Model 640 and 641 High Performance Suppressors Continuous regeneration for automated runs Stand-alone units are easy to add to any IC or HPLC system Patented technology improves sensitivity, eliminates the water dip and improves baseline stability Meets requirements of EPA Methods and Suppressors reduce background conductance of the mobile phase while simultaneously increasing the analyte signal, for ppb-level anion detection. Alltech s patented* regeneration and degassing features make our suppressors the most reliable, flexible suppressor systems available. You won t find these critical features on any other suppressor. Reagent-free regeneration no down-time, no chemicals, no extra equipment Alltech s suppressor cell continuously regenerates and is always ready for the next run. You need no extra reagents or regeneration steps, and never have to interrupt a run to regenerate or change cells. Alltech s suppressors make it easy to automate your procedures. Automatic degassing reduce or eliminate water dips and baseline shifts Carbonate/bicarbonate mobile phases are powerful separation tools, but produce carbon dioxide during suppression, which can interfere with conductivity detection. Alltech s suppressors automatically degas the effluent, leaving your sample in pure, non-conductive water for maximum sensitivity. This lets you enjoy all the advantages of carbonate mobile phases (even gradients with the Model 641) without the disadvantages. Add IC detection to any HPLC system With Alltech s suppressors and a conductivity detector, you can add high-performance IC capability to your favorite HPLC system. Our suppressors are simple to operate - say goodbye to long equilibration times and multiple operating modes! *U.S. patent number 6,444,475 6,468,804. Other patents pending. Model 640 Suppressor for isocratic separations. Model 641 Suppressor for gradient separations. Two models to choose from Choose the Model 640 for isocratic applications up to 20mM sodium hydroxide or 10mM sodium carbonate. Choose the Model 641 for gradient applications up to 40mM sodium hydroxide or 20mM sodium carbonate. Simplify methods by using fewer columns and mobile phases Alltech s suppressors streamline your IC methods. Use powerful, selective carbonate/bicarbonate mobile phases (even gradients with the Model 641) and just one or two column chemistries for almost any anion separation. With carbonate/bicarbonate, the separating power is in the mobile phase, so fewer column types are needed to perform most separations. Specifications Display: LED Indicators Fluid path: PEEK and PTFE Power: 120/240 VAC, Hz Outputs: Contact Closure (NO or NC) on error Dimensions: 5.06" H x 10.33" W x 19" D, (12.9cm H x 26.2cm W x 48.3cm D) Weight: 19.5lbs (8.8kg) Capacity: Model 640: 20mM sodium hydroxide, 10mM sodium carbonate Model 641: 40mM sodium hydroxide, 20mM sodium carbonate Warranty: 1 year parts and labor, 6 months on suppressor cell

20 I N S T R U M E N T S Suppressors Trace Anions in Morpholine Fluoride (0.6ppb) 2. Chloride (5ppb) 3. Nitrate (3ppb) 4. Sulfate (2ppb) Min. With Alltech Suppressors Min. With Other Suppressors Column: IonPac AS9-HC, 4.0 x 250mm Mobile Phase: 9.0mM Na2CO3 Flow Rate: 1.0mL/min Detector: Suppressed Conductivity Alltech s patented degassing feature improves sensitivity, baseline stability, and resolving power. Inorganic Anions and Oxyhalides EPA Method 300.1, Part B Fluoride (1 ppm) 2. Chlorite (0.5ppm) 3. Bromate (0.05ppm) 4. Chloride (50ppm) 5. Nitrite (0.1ppm) 6. Bromide (0.05ppm) 7. Chlorate (0.05ppm) 8. Nitrate (10ppm) 9. Phosphate (0.1ppm) 10. Sulfate (50ppm) Column: IonPac AS9-HC, 4.0 x 250mm Mobile Phase: 9.0mM Na2CO Min Min. Flow Rate: 1.0mL/min Detector: Suppressed Conductivity With Alltech Suppressors With Other Suppressors 10 Alltech suppressors dramatically reduce and often eliminate the water dip that interferes with early eluting peaks. Need An Affordable System? With these new, low cost suppressors, adding a complete IC detection system to your lab is easy and affordable! See page 326 for details. ALLTECH SUPPRESSORS DESCRIPTION Model 640 Suppressor (Isocratic) 120V V Model 641 Suppressor (Gradient) 120V V

21 Suppressors I N S T R U M E N T S Model 335 Suppressor Meets U.S. EPA Method requirements Convenient disposable cartridges Low Level Anions per US EPA Method Fluoride 2. Chloride 3. Nitrite 4. Bromide 5. Nitrate 6. Phosphate 7. Sulfate 8542 Installs easily in any IC or HPLC system 2329 Column: Allsep Anion, 7µm, 4.6 x 100mm (Part No ) Mobile Phase: 0.85mM NaHCO3/0.9mM Na2CO3 Flow Rate: 2.0mL/min Detector: Suppressed Conductivity Add the Model 335 Ion Suppressor to any IC or LC system that has a conductivity detector. Explore new separations or run existing suppressor-based methods, including EPA Method part A and B. This suppressor uses two disposable suppressor cartridges. While one cartridge provides suppression, the other is equilibrated or replaced. Changing the disposable cartridge is fast and easy. Tap Water Analysis Fluoride 2. Chloride 3. Nitrite 4. Nitrate 5. Sulfate Model 335 Suppressor The Model 335 Suppressor is ideal for educational labs and labs that do not frequently use Ion Chromatography. For fully unattended, continuously regenerated suppression, see our Model 640 and Model 641 Suppressors on pages Column: Allsep Anion, 7µm, 4.6 x 100mm (Part No ) Mobile Phase: 0.85mM NaHCO3/0.9mM Na2CO3 Flow Rate: 1.2mL/min Detector: Suppressed Conductivity 2 For more information on the 335 Suppressor, request instrument support sheet #019. Also available on our website. MODEL 335 SUPPRESSOR MODULE DESCRIPTION Model 335 Suppressor Module (Includes 25 Anion Cartridges) Anion SPCS Cartridges, 25/pk

22 I N S T R U M E N T S Conductivity Detector Model 650 Conductivity Detector and Model 630 Column Heater Precise temperature control gives stable, drift-free baselines Detects charged species at ppb levels Compatible with suppressor-based and nonsuppressed methods Model 650 Conductivity Detector Specifications Detection: Alternating current, synchronous detection, 3KHz frequency Range: µS in 12 steps Display: Graphical LCD Autozero: Offsets up to 10,000µS Time Constant: 0.1, 0.5, 1, 5, 10sec, software selectable Method Storage: 0-9, user defined Output: 10mV, 100mV, 1V Full Scale Computer Interface: RS-232 Serial Communications Fluid Path: PTFE Dimensions: 10.5" W x 19" L x 5.5" H (26.7cm x 48cm x 14cm) Cell Compartment Size: 2.25" W x 6.25" L x 2.25" H (5.7cm x 15.9cm x 5.7cm) Insulation: Minimum 1" (2.54cm) all sides Heater: 25W DC heating element vulcanized to aluminum block Preheater: PTFE tubing knitted and encased in high thermal mass housing Cell Temperature: Factory set at 35 C, User adjustable ambient to 60 C Temp. Precision: ±0.05 C Cell Type: 2 electrode, gold-plated SS electrodes Cell Volume: 0.5µL Pressure Rating: 400psig Fluid Path: PTFE Use the conductivity detector alone, or in conjunction with its dedicated column heater for the best control and reproducibility of your results. Unlike other conductivity detectors, the Model 650 Conductivity Detector can discriminate between random noise and the analyte signal, improving sensitivity. State-of-the-art signal processing and precise temperature control of the detection cell give stable, low-noise baselines. You can easily program and store up to 10 methods through the front panel controls. You can further improve your results by using the Model 630 Column Heater to eliminate problems caused by fluctuating column temperatures. The Model 630 Column Heater cannot be used as a stand-alone column heater; it is programmed and controlled by the Model 650 Conductivity Detector. Not sure you need a column heater? You can always add it to your system later Model 630 Column Heater Specifications Cavity Dimensions: 1.8" H x 5.0" W x 16.5" D (4.5 H x 12.7 W x 41.9 D cm) Preheater: 2' x 0.01" i.d. PEEK coil cast in tin alloy block Temperature Control: Ambient to 60 C Temperature Accuracy: ±2.5 C Temperature Stability: ±1.25 C Operating Voltage: 115V, 230V Fluid Path Construction: PEEK Dimensions: 5.0" H x 10.3" W x 19.5" D (13cm H x 26cm W x 50cm D) MODEL 650 CONDUCTIVITY DETECTOR & COLUMN HEATER DESCRIPTION Model 650 Conductivity Detector 120V V Model 630 Column Heater 120V V See page 326 for special pricing on detection systems. Do you need a stand-alone column heater? See page 298 to learn about the Model 631 Column Heater. 325

23 Systems I N S T R U M E N T S IC Detection System Packages Save 20% off individual module price Compatible with suppressor-based and non-suppressed methods Modular-design is easy to change and upgrade Choose the Model 650 Conductivity Detector in combination with other components to easily and economically add IC capability to your existing HPLC equipment. You can quickly switch between HPLC and IC applications. For Cation Analysis Model 650 conductivity detector Model 630 column heater For Cation and Anion Analysis Model 650 conductivity detector Model 630 column heater Choice of model 640 or 641 suppressors CATION DETECTION SYSTEMS DESCRIPTION Cation Detection System Includes: Conductivity Detector and Column Heater 110V V For Anion Analysis Model 650 conductivity detector Choice of model 640 or 641 suppressors CATION AND ANION DETECTION SYSTEMS DESCRIPTION Cation and Anion Isocratic Detection System Includes: Conductivity Detector, Column Heater, & Model 640 Suppressor 110V V Cation and Anion Gradient Detection System Includes: Conductivity Detector, Column Heater, & Model 641 Suppressor 110V V ANION DETECTION SYSTEMS DESCRIPTION Anion Isocratic Detection System Includes: Conductivity Detector and Model 640 Suppressor 110V V Anion Gradient Detection System Includes: Conductivity Detector and Model 641 Suppressor 110V V For added savings and convenience, choose an IC starter kit from Page 317! Alltech s anion and cation starter kits contain the columns, guard columns, and mobile phases to perform the most common anion and cation analyses. Each kit includes an Alltech IC column, matched guard column, EZ-Lute mobile phase buffer concentrate, and IC standards. 326

24 I N S T R U M E N T S Systems Ion Chromatography System Components Buy Only the Components You Need or Build a Complete System Now you can easily and affordably add IC detection to your existing HPLC equipment with Alltech s conductivity detector and new low-cost, high-performance suppressors. Need a whole system? Alltech has all the components you need to build a complete system! On-Line Degassing Systems Most advanced degassing technology Choose from two families for basic or advanced features Choose from three flow rates, up to 10mL/min Upgrade the number of channels as your needs change 6488 See pages to learn more about Alltech s On-Line degassing Systems. Pumps Pumps to fit all needs and budgets Available in stainless steel or PEEK High-pressure gradients (up to quaternary) See pages to learn more about Alltech s complete line of HPLC pumps Autosamplers Improve laboratory efficiency Basic or advanced capabilities Available in stainless steel or PEEK See page 293 to learn more about Alltech s autosamplers UV Detectors Use for specialized IC applications Choose from fixed, variable, and programmable dual wavelength detectors Interchangeable flowcells and lamps See pages to learn more about Alltech s UV detectors EZStart Data Acquisition Software PC-based data handling and system control Optional control of instruments from many vendors Easy to use See page 301 and to learn more about EZStart Data Acquisition Software. EZStart02a 327