Dionex IonPac AS28-Fast-4µm column
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1 CHROMATOGRAPHY Thermo Scientific Dionex IonPac AS-Fast-4µm Columns Product Specifications The Thermo Scientific Dionex IonPac AS-Fast-4µm column is a high-capacity, hydroxide-selective anionexchange column designed for the determination of trace-level concentrations of inorganic anions and low molecular weight organic acids, including fluoride, glycolate, acetate, formate, chloride, nitrite, carbonate, sulfate, oxalate, bromide, nitrate, and phosphate in highpurity water matrices encountered in the semiconductor and power generation industries. The Dionex IonPac AS-Fast-4µm column offers excellent retention of fluoride from the water dip and uses a simple hydroxide eluent easily supplied by the Eluent Generator (EG)..0 Gradient Separation of Trace Organic Acids and Inorganic Anions on a Dionex IonPac AS-Fast-4µm (4 0 mm) Column 4 0 Peaks: µg/l (ppb). Fluoride.0. Acetate.0. Glycolate.0 4. Formate.0. Chloride.. Nitrite.0. Carbonate.0. Sulfate.0. Oxalate.0 0. Bromide 0.0. Nitrate 0.0. Phosphate.0 The Dionex IonPac AS-Fast-4µm column is available in formats from 0.4 to 4 mm, allowing use of capillary to analytical flow rates, and supporting advanced IC IC applications Superior Chromatographic Performance Universal column for trace-level concentrations of inorganic anions and low molecular weight organic acids in high-purity water matrices. The Dionex IonPac AS-Fast-4µm column offers improved peak shapes, efficiencies, and resolution compared to the Dionex IonPac AS column and is the recommended replacement for the Dionex IonPac AS column. Easily transfer applications from the Dionex IonPac AS column to the Dionex IonPac AS-Fast-4µm column due to the similar selectivity. The Dionex IonPac AS-Fast-4µm Capillary column (0.4 0 mm) offers reduced eluent consumption, reduced operating costs, and excellent reproducibility. Column selectivity optimized for a 0 C operating temperature to ensure reproducible retention times. Large-loop injections for ppb-level analysis. Superior retention and resolution of early-eluting anions, including fluoride, glycolate, acetate, and formate. Simplified operation is provided by the EG, which requires only a deionized water source to produce potassium hydroxide eluent. Hydroxide eluent suppresses to a very low background for trace-level determinations. Compatible with organic solvents to enhance analyte solubility, modify column selectivity, and facilitate effective column cleanup. Direct transfer of 4 mm applications to the mm and 0.4 mm column formats for more economical operation, reduced eluent consumption, and increased mass sensitivity.
2 High-Efficiency Particle Structure The Dionex IonPac AS-Fast-4µm packing is a unique structure composed of a highly crosslinked core and a MicroBead anion-exchange layer attached to the surface, as shown in Figure. The Dionex IonPac AS-Fast-4µm column substrate is composed of a supermacroporous 4 µm diameter resin bead consisting of ethylvinylbenzene crosslinked with % divinylbenzene. The anion-exchange layer is functionalized with alkanol quaternary ammonium groups and has a controlled thickness, which results in excellent mass transfer characteristics and consequently, highly efficient peaks. Versatile Capability The Dionex IonPac AS-Fast-4µm column is ideal for the isocratic separation of fluoride, chloride, carbonate, nitrite, sulfate, bromide, nitrate, and phosphate in high purity water samples using a simple hydroxide eluent and suppressed conductivity detection. Figure illustrates the excellent separation of chloride from carbonate, with highly retained analytes, such as phosphate, eluting in less than minutes. 4µm Capillary Format The Dionex IonPac AS-Fast-4µm Capillary column (0.4 0 mm) is packed with the same material as the equivalent standard bore version (producing the same performance as a 4 mm column), but requires only /00th the eluent flow rate. The capillary format offers the advantage of less eluent consumption providing reduced operating and disposal costs. Figure illustrates the effect of flow rate on run time using an isocratic eluent and the Dionex IonPac AS-Fast-4µm (0.4 0 mm) capillary column. By increasing the flow rate, common anions can be analyzed in under minutes with no change in selectivity. Excellent retention time reproducibility can be achieved with the capillary format. Figure. Structure of a Dionex IonPac AS-Fast-4µm packing particle Eluent: 0 mm KOH Eluent: mm KOH Figure. Effect of eluent concentration on the isocratic separation of common anions using the Dionex IonPac AS-Fast-4µm ( 0 mm) column Flow Rate: µl/min Flow Rate: 0 µl/min Flow Rate: µl/min AS-Fast-4µm ( 0 mm) Eluent: See Chromatogram Eluent Source: Dionex EGC 00 KOH Flow Rate: 0. ml/min Inj. Volume:. µl Temperature: 0 C Dionex AERS 00 ( mm) Peaks: mg/l. Fluoride.0. Chloride.0. Carbonate 4. Nitrite 0.0. Sulfate 0.0. Bromide 0.0. Nitrate 0.0. Phosphate 0.0 AS-Fast-4µm (0.4 0 mm) Eluent: mm KOH Eluent Source: Dionex EGC 00 KOH Flow Rate: See Chromatogram Inj. Volume: 0.4 µl Temperature: 0 C Dionex ACES 00 Peaks: mg/l. Fluoride 0.. Chloride.. Carbonate 4. Nitrite.. Sulfate.. Bromide.0. Nitrate.0. Phosphate. Figure. Isocratic separation of common anions using the Dionex IonPac AS-Fast-4µm (0.4 0 mm) capillary column at various flow rates.
3 Large Loop Injections for μg/l (ppb) Level Analysis The selectivity and high capacity of the Dionex IonPac AS-Fast-4µm column allows the determination of trace levels of inorganic anions and low molecular weight organic acids in high-purity water samples using a large-loop injection. Figure 4 illustrates the separation of inorganic anions and low molecular weight organic acids in a high-purity water sample using this technique. Separation was achieved using a potassium hydroxide gradient with suppressed conductivity detection at a temperature of 0 C. Low μg/l (ppb) levels of these analytes can easily be quantified using a ml injection loop with a 4 mm column, a 00 µl injection loop with a mm column, or a 0 µl injection loop with a 0.4 mm capillary column. The conductivity of the hydroxide eluent can be suppressed to a very low background, facilitating trace-level analysis. Extended Application Capabilities The unique selectivity and high capacity of the Dionex IonPac AS-Fast-4µm column make it an ideal column for methods development of specialized anion applications. For example, anionic additives in personal care products can easily be determined. Figure illustrates the excellent separation of fluoride, chloride, monofluorophosphate, sulfate, and phosphate found in dental care products. Trace Analysis is Easier with an Eluent Generator Combining the Dionex IonPac AS-Fast- 4µm column with an EG makes trace anion analysis even easier. An EG makes potassium hydroxide eluent electrolytically from water. The use of this ultrapure hydroxide as an eluent results in a low background and stable baseline.gradients can be programmed with ease on an EG, facilitating method optimization. Figure illustrates the optimization of a hydroxide gradient and flow rate to reduce analysis time of low molecular weight organic acids and inorganic anions commonly encountered in the chemical, semiconductor, and power generation industries Eluent: mm KOH from 0 to 4 minutes, -0 mm KOH from 4 to minutes, 0- mm KOH from to0 minutes, mm KOH from 0 to 0 minutes Flow Rate: µl/min Eluent: mm KOH from 0 to minutes, Flow Rate: µl/min -0 mm KOH from to minutes, 0- mm KOH from to minutes, 0 mm KOH from to minutes 4 Eluent: -0 mm 0- mm KOH from to minutes, mm KOH from to minutes Standard Toothpaste Sample A Toothpaste Sample B AS-Fast-4µm ( 0 mm) Eluent: mm KOH from 0 to mins, -0 mm KOH from to min, 0- mm KOH from to min, mm KOH from to min Eluent Source: Dionex EGC 00 KOH Flow Rate: 0. ml/min Inj. Volume: 00 µl Temperature: 0 C Dionex AERS 00 ( mm) Suppressor, AutoSuppression, Peaks: µg/l (ppb). Fluoride.0. Carbonate.0. Acetate.0. Sulfate.0. Glycolate.0. Oxalate.0 4. Formate.0 0. Bromide 0.0. Chloride.. Nitrate 0.0. Nitrite.0. Phosphate.0 Figure 4. Determination of trace organic acids and inorganic anions using a hydroxide gradient, large loop injection, and the Dionex IonPac AS-Fast-4µm ( 0 mm) column. AS-Fast-4µm ( 0 mm) Eluent: mm KOH from 0 to mins, -0 mm KOH from to mins, 0- mm KOH from to mins, mm KOH from to mins Eluent Source: Dionex EGC 00 KOH Flow Rate: 0. ml/min Inj. Volume:. µl Temperature: 0 C Dionex AERS 00 ( mm) Peaks: mg/l (ppm). Fluoride.0. Acetate 0.. Glycolate Formate 0.. Chloride 0.. Carbonate. Monofluorophosphate.0. Sulfate.0. Phosphate.0 Figure. Determination of anionic additives in toothpaste using the Dionex IonPac AS-Fast-4µm ( x 0 mm) column. AS-Fast-4µm (0.4 0 mm) Eluent: See Chromatogram Eluent Source: Dionex EGC 00 KOH Flow Rate: See Chromatogram Inj. Volume: 0.4 µl Temperature: 0 C Dionex ACES 00 Suppressor, AutoSuppression, Peaks: mg/l (ppm). Fluoride 0.. Carbonate.. Acetate.. Sulfate.. Glycolate.. Oxalate. 4. Formate. 0. Bromide.0. Chloride 0.. Nitrate.0. Nitrite.. Phosphate. Figure. Optimization of hydroxide gradient and flow rate to reduce analysis time with the Dionex IonPac AS-Fast-4µm (0.4 0 mm) capillary column.
4 4 Trace Analysis of Inorganic Anions in High Ionic Strength Matrices High capacity columns allow large loop injections while maintaining the peak shapes of trace components, even in the presence of high concentrations of other anions (e.g., carbonate) during purity analysis of salts such as sodium bicarbonate and sodium carbonate. Figure shows the use of a large loop injection on the Dionex IonPac AS-Fast- 4µm ( 0 mm) column for trace analysis of chloride and sulfate in samples of sodium bicarbonate. Using an isocratic potassium hydroxide eluent, the bicarbonate peak is well resolved from chloride and sulfate and does not interfere with quantification of these trace anions. Figure shows the purity analysis of sodium carbonate using a large loop injection on the Dionex IonPac AS-Fast-4µm ( 0 mm) column. Determination of Inorganic Anions and Low Molecular Weight Organic Acids Low molecular weight organic acids and mono- and divalent inorganic anions commonly encountered in industrial process solutions and chemicals can be determined in a single run using the Dionex IonPac AS-Fast-4µm column. Figure illustrates the separation of weakly retained anions such as acetate, butyrate, and formate in a simulated industrial chemical sample using a hydroxide gradient at a controlled temperature of 0 C. Vendor B Vendor A AS-Fast-4µm ( 0 mm) Eluent: 0 mm KOH Eluent Source: Dionex EGC 00 KOH Flow Rate: 0. ml/min Inj. Volume: 00 µl Temperature: 0 C Dionex AERS 00 ( mm) Sample 000 ppm sodium bicarbonate Peaks:. Chloride. Bicarbonate. Sulfate Figure. Purity analysis of sodium bicarbonate using a large loop injection on the Dionex IonPac AS-Fast-4µm ( 0 mm) column. AS-Fast-4µm ( 0 mm) Eluent: 0 mm KOH Eluent Source: Dionex EGC 00 KOH Flow Rate: 0. ml/min Inj. Volume: 00 µl Temperature: 0 C Dionex AERS 00 ( mm) Sample Peaks: ppm sodium carbonate. Chloride. Carbonate. Sulfate Figure. Purity analysis of sodium carbonate using a large loop injection on the Dionex IonPac AS-Fast-4µm ( 0 mm) column Dionex IonPac AS-Fast-4µm (4 0 mm) Eluent: mm KOH from 0 to minutes, -0 mm KOH from to min, 0- mm KOH from to min, mm KOH from to min Eluent Source: Dionex EGC 00 KOH Flow Rate:. ml/min Inj. Volume: 0 µl Temperature: 0 C Detection: Dionex AERS 00 (4 mm) Suppressor AutoSuppression, recycle mode Peaks: mg/l (ppm). Fluoride.0 4. Sulfate.0. Acetate.0. Oxalate.0. Iodate.0. Benzoate Butyrate.0. Azide 0.0. Formate.0. Bromide 0.0. Valariate.0. Nitrate 0.0. Methylsulfonate.0 0. Chlorate 0.0. Chlorite.0. Phosphate 0.0. Bromate.0. Arsenate Chloride.. Citrate 0.0. Nitrite.0 4. Chromate 0.0. Carbonate.0. Thiosulfate 0.0. Monofluorophosphate 0.0 Figure. Separation of various environmental anions using a hydroxide gradient and the Dionex IonPac AS-Fast-4µm (4 0 mm) column.
5 System Recommendations The Dionex IonPac AS-Fast-4µm column is recommended for use with Thermo Scientific Dionex ICS-4000, Thermo Scientific Dionex ICS-000 +, Thermo Scientific Dionex ICS-000, or Thermo Scientific Dionex Integrion HPIC systems equipped with an EG. The EG is used to automatically produce potassium hydroxide gradients from deionized water. For all systems, the use of Thermo Scientific Dionex IC PEEK Viper fittings is recommended to achieve consistent low dead volume connections and ensure optimum chromatographic performance. Suppressor Recommendations For optimum ease of use and economy, use the Dionex IonPac AS-Fast-4µm column with the Thermo Scientific Dionex ADRS 00 Anion Dynamically Regenerated Suppressor or Thermo Scientific Dionex ACES 00 Anion Capillary Electrolytic Suppressor. Operate the Dionex IonPac AS-Fast-4µm column at 0 C to ensure reproducible retention times. Anion Trap Columns When using the EG for eluent delivery, a Thermo Scientific Dionex CR-ATC 00 or CR-ATC 00 Continuously Regenerated Anion Trap Column column should be installed between the EG cartridge and the EG degas module. Alternately, 4 mm and mm systems can use the Thermo Scientific Dionex IonPac ATC-HC 00 Anion Trap Column, installed between the pump outlet and inlet of the Dionex EluGen cartridge in the Dionex EG module. When performing sodium hydroxide gradient applications on the Dionex IonPac AS- Fast-4µm column using manually prepared eluents, the ATC 00 should be installed between the gradient pump and the injection valve to remove anionic contaminants from the eluent. Concentrator Columns For concentrator work, when a single piston pump such as the AXP pump (pulse damper required) is used for sample delivery, use the Dionex IonPac AG-Fast-4µm Guard column, Thermo Scientific Dionex IonPac UTAC- Series Ultra Trace Anion Concentrator Columns (UTAC-LP, UTAC-ULP, or UTAC-XLP), or the Thermo Scientific Dionex IonPac TAC- Trace Anion Concentrator Columns. Use the IonPac UTAC LP, UTAC-LP or the TAC-LP when the sample is delivered with a syringe or with an autosampler. For concentrator work with a 0.4 mm capillary column, use the Dionex IonPac AG-Fast-4µm Capillary Guard column or the Thermo Scientific Dionex IonSwift MAC-00 Concentrator column. Specifications Dimensions Dionex IonPac AS-Fast-4µm Capillary Column mm Dionex IonPac AG-Fast-4µm Capillary Guard Column 0.4 mm Dionex IonPac AS-Fast-4µm Analytical Columns 0 and 4 0 mm Dionex IonPac AG-Fast-4µm Guard Columns 0 and 4 0 mm Maximum Operating Pressure 000 psi (Standard, Microbore, and Capillary) Mobile Phase Compatibility ph 0 4; 0 00% acetonitrile, methanol, and isopropanol Substrate Characteristics Bead Diameter: 4 µm (Analytical/Capillary column)* µm (Guard/Capillary Guard column)** *Analytical/Capillary Column resin composition: supermacroporous ethylvinylbenzene polymer cross-linked with divinylbenzene **Guard/Capillary Guard Column resin composition: microporous ethylvinylbenzene polymer cross-linked with divinylbenzene Crosslinking (%DVB): % Ion-Exchange Group Surface-functionalized with alkanol quaternary ammonium ion Functional Group Characteristic Medium high hydrophobicity Capacity 0 µeq/column (4 0 mm). µeq/column ( 0 mm). µeq/column (0.4 0 mm) 0 µeq/column (4 0 mm) µeq/column ( 0 mm) 0. µeq/column (0.4 mm)
6 Ordering Information In the U.S., call or contact the Thermo Fisher Scientific Regional office nearest you. Outside the U.S., order through your local Thermo Fisher Scientific office or distributor. Refer to the following part numbers. Capillary, Analytical, and Guard Columns Part Number Dionex IonPac AS-Fast-4µm Capillary Column mm 0 Dionex IonPac AG-Fast-4µm Capillary Guard Column 0.4 mm 0 Dionex IonPac AS-Fast-4µm Analytical Column 4 0 mm 04 Dionex IonPac AG-Fast-4µm Guard Column 4 0 mm 04 Dionex IonPac AS-Fast-4µm Analytical Column 0 mm 04 Dionex IonPac AG-Fast-4µm Guard Column 0 mm 00 Dionex IC PEEK Viper Fittings Kits Dionex IC PEEK Viper Fittings Kit for Dionex Integrion HPIC systems with conductivity detectors 0 Dionex IC PEEK Viper Fittings Kit for Dionex ICS /ICS-000 systems with conductivity detectors 00 Dionex IC PEEK Viper Fittings Kit for Dionex ICS /ICS-000 capillary systems with conductivity detectors 00 Dionex IC PEEK Viper Fittings Kit for Dionex ICS-4000 capillary systems with conductivity detectors 0 Anion Trap Columns Dionex CR-ATC 00 Continuously Regenerated Anion Trap Column, for use with Dionex Integrion HPIC systems equipped with an eluent generator 0 Dionex CR-ATC 00 Continuously Regenerated Anion Trap Column, for use with Dionex ICS systems equipped with an eluent generator 00 Dionex CR-ATC Continuously Regenerated Anion Trap Column (Capillary), for use with capillary HPIC systems 00 Dionex ATC-00 Anion Trap Column ( 4 mm), for use with manually prepared eluents and 4 mm columns) 0 Dionex ATC-00 Anion Trap Column (4 mm), for use with manually prepared eluents and mm columns) 00 Dionex ATC-HC 00 Anion Trap Column ( mm) 0 Trace Anion Concentrator Columns Dionex IonSwift MAC-00 Monolith Anion Concentrator Column (0. 0 mm) 040 Dionex IonPac TAC-LP Trace Anion Concentrator (4 mm) 040 Dionex IonPac UTAC-LP Ultra Trace Anion Concentrator Low Pressure (4 mm) 00 Dionex IonPac UTAC-LP Ultra Trace Anion Concentrator Low Pressure (4 mm) 0 Dionex IonPac UTAC-ULP Ultra Trace Anion Concentrator Ultra Low Pressure ( mm) 0 Dionex IonPac UTAC-XLP Ultra Trace Anion Concentrator Extremely Low Pressure ( mm) 0 Product Specifications Find out more at thermofisher.com/ic 0 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries. This information is presented as an example of the capabilities of Thermo Fisher Scientific products. It is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representatives for details. PS04-EN 0S
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