Thermo Scientific Dionex IonPac AS11 and AS11-HC Anion-Exchange Column

Transcription

1 chromatography Separate a wide variety of inorganic anions and organic acids using the Dionex IonPac AS and AS-HC Anion-Exchange Columns with potassium hydroxide gradients. Product Specifications Thermo Scientific Dionex IonPac AS and AS-HC Anion-Exchange Column. Quinate. mg/l. Methylsulfonate.. Flouride.. Pyruvate.. Lactate.. Valerate.. Acetate.. Monochloroacetate.. Propionate.. Bromate.. Formate.. Chloride.. Butyrate.. Nitrite.. Triflouroacetate.. Bromide.. Nitrate.. Carbonate. Malonate.. Maleate.. Sulfate.. Oxalate.. Tungstate.. Phosphate.. Pthalate.. Citrate.. Chromate.. cis-aconitate. trans-aconitate. Column: Dionex IonPac AG-HC/AS-HC (. mm) Eluent Source: Thermo Scientific Dionex EGC-KOH (Capillary) Eluent: Potassium hydroxide (EGC) mm from to min, mm to mm from to min, mm to mm from to min, mm to mm from to min Flow Rate:. ml/min Inj. Volume:. µl Temperature: C Thermo Scientific Dionex ACES, AutoSuppression recycle mode The Dionex IonPac AS and AS-HC anion-exchange columns are designed for the separation of organic acids and inorganic anions in various matrices. Simpler matices can quickly be profiled using the AS while the AS-HC is best for more complex, higher ionic strength matrices. The Dionex IonPac AS anionexchange column provides fast profiling of inorganic anions and organic acid anions using sodium hydroxide or potassium hydroxide gradient elution. The AS column is available in mm and mm formats. The Dionex IonPac AS-HC is a high-capacity anion-exchange column with selectivity similar to the AS column. This high-capacity column is designed to resolve a large number of organic acids and inorganic anions in complex matrices, using potassium hydroxide gradient elution. The Dionex IonPac AS-HC is ideal for the determination of trace components and for profiling organic acids in uncharacterized samples, and is available in mm, mm, and. mm formats. Part of Thermo Fisher Scientific

2 Dionex IonPac AS Anion-Exchange Column Highlights Separation of a wide variety of organic acids and inorganic anions in various matrices Faster profiling of simple matrices using the Dionex IonPac AS Injection of more concentrated samples without overloading and peak broadening using the high-capacity Dionex IonPac AS-HC column Dionex IonPac AS Features Using a hydroxide gradient of. to mm sodium hydroxide, the AS column separates a large number of inorganic anions and organic acids in approximately min, as illustrated in Figures A and B. Peak broadening and overloading can occur when higher concentrations of analytes are injected onto the AS column, as shown in Figure A. These problems can be eliminated by diluting the sample before injection, as shown in Figure B. Conversely, the high-capacity AS-HC column allows more concentrated samples to be directly injected without overloading the column or affecting peak shape, as illustrated in Figure C. The AS also provides rapid elution of strongly retained ions such as iodide, thiocyanate, and thiosulfate in complex matrices. Dionex IonPac AS-HC Features The high-capacity AS-HC column allows the injection of more concentrated samples without overloading and peak broadening. The AS HC provides improved separation over the AS column for monovalent carboxylic acids, including quinate, lactate, acetate, propionate, formate, and butyrate. A wide range of organic acids and inorganic anions can be separated in approximately min using a sodium hydroxide gradient at a controlled temperature of C, as illustrated in Figure C. Columns A & B: Dionex IonPac AG, AS, mm Column C: Dionex IonPac AG-HC, AS-HC, mm Eluent: Sodium hydroxide:. mm from to min,. mm to mm from to min, mm to mm from Eluent: Sodium hydroxide: mm from to min, mm to mm from to min, mm to mm from to min to min Flow Rate:. ml/min Flow Rate:. ml/min Temperature: C Temperature: C Inj. Volume: µl Inj. Volume: µl Dionex ASRS -ULTRA mm, Thermo Scientific Dionex ASRS-ULTRA mm, AutoSuppression recycle mode AutoSuppression recycle mode A B C B A & C. Quinate. mg/l (ppm). Fluoride.. Lactate.. Acetate.. Propionate.. Formate.. Butyrate.. Methylsulfonate.. Pyruvate.. Chlorite.. Valerate.. Monochloroacetate.. Bromate.. Chloride.. Nitrite.. Trifluoroacetate.. Bromide.. Nitrate.. Carbonate. Malonate.. Maleate.. Sulfate.. Oxalate.. Ketomalonate. Tungstate. Phosphate. Phthalate. Citrate. Chromate. cis-aconitate. trans-aconitate Figure. Comparison of the Dionex IonPac AS and AS-HC columns for the separation of a wide range of organic acids and inorganic anions using sodium hydroxide gradient elution. The high capacity AS-HC provides excellent peak shape for early-eluting anions.

3 Key Applications The Dionex IonPac AS and AS-HC columns are solvent-compatible, which allows for anion-exchange selectivity control and easy column cleanup after the analysis of complex matrices. Typical applications include the determination of organic acids and inorganic anions in diverse sample matrices including: Fruit juices and wines Foods and beverages Fermentation and process solutions Chemical additives Chemical process solutions Wastewater Brines Power plant waters Economical Capillary Operation The Dionex IonPac AS-HC column is also available in the. mm for mat for capillary operation to offer the advantage of reduced operating costs. Ideal for limited sample volumes due to higher mass sensitivity. One hundred fold reduction in eluent consumption and waste disposal. mm applications can be directly transferred to the. mm format by reducing flow rate by one hundred fold. Figure is an example of the separation of inorganic anions and organic acids using the Dionex IonPac AS-HC capillary column. Column: Dionex IonPac AG-HC/AS-HC (. mm) Eluent Source: Dionex EGC-KOH (Capillary) Eluent: Potassium hydroxide mm from to min, mm to mm from to min, mm to mm from to min, mm to mm from to min Flow Rate:. ml/min Inj. Volume:. µl Temperature: C Dionex ACES, AutoSuppression recycle mode. Quinate. mg/l. Flouride.. Lactate.. Acetate.. Propionate.. Formate.. Butyrate.. Methylsulfonate.. Pyruvate.. Valerate.. Monochloroacetate.. Bromate.. Chloride.. Nitrite.. Triflouroacetate.. Bromide.. Nitrate.. Carbonate. Malonate.. Maleate.. Sulfate.. Oxalate.. Tungstate.. Phosphate.. Pthalate.. Citrate.. Chromate.. cis-aconitate. trans-aconitate. Figure. Gradient separation of inorganic anions and organic acids using the Dionex IonPac AS-HC capillary column. Anion-Exchange Latex Coating. m Highly Crosslinked Core nm Diameter MicroBeads High-Efficiency Particle Structure The Dionex IonPac AS and AS-HC column packings have unique structures composed of a highly crosslinked core and a MicroBead anion-exchange layer attached to the surface, as shown in Figures and. The substrate for the Dionex IonPac AS is a µm diameter microporous resin bead. The Dionex IonPac AS-HC column substrate is a µm diameter macroporous resin bead. Both columns are composed of ethylvinylbenzene crosslinked with % divinylbenzene. The anion-exchange layer is functionalized with quaternary ammon ium groups. This anion-exchange layer has a controlled thickness, which results in excellent masstransfer characteristics and, consequently, very high efficiency peaks. Ethylvinylbenzene-Divinylbenzene Core Figure. Structure of a Dionex IonPac AS packing particle. Anion-Exchange Latex Coating Highly Crosslinked Core -nm Diameter MicroBeads Ethylvinylbenzene-Divinylbenzene Core Figure. Structure of an Dionex IonPac AS-HC packing particle.

4 Dionex IonPac AS Anion-Exchange Column Solvent-Compatible Packing Because the Dionex IonPac AS and AS-HC columns are % HPLC solvent compatible, organic solvents can be used for efficient column cleanup or to enhance sample solubility. Time and expense can be saved through the elimination of time-consuming sample preparation steps. This feature allows complex matrices to be analyzed with minimal sample preparation and extends the utility of the column to new applications requiring solvents. Adding organic solvents to the eluent modifies column selectivity and enables the elution of nonpolar analytes or contaminants from the column. Dionex IonPac AS Column for Fast Gradient Profile of Organic Acid Anions The Dionex IonPac AS column provides fast profiling of organic acids and inorganic anions using sodium or potassium hydroxide gradient elution. The column is ideal for the determination of organic acids and inorganic anions at similar concentrations or organic acids in well-characterized samples. Mono-, di-, and trivalent organic acids as well as inorganic anions can be separated in approximately min using a sodium or potassium hydroxide gradient. Determination of Short-Chain Organic Acids Weakly retained organic acids and inorganic anions commonly encountered in the food, beverage, biopharmaceutical, chemical, and power industries can be determined in a single run using the Dionex IonPac AS-HC column. Resolution of monovalent organic acids and improved peak shape are two benefits of the high capacity of the Dionex IonPac AS-HC. Column A: Dionex IonPac AG, AS, mm Column B: Dionex IonPac AG-HC, AS-HC, mm Eluent: Sodium hydroxide:. mm from to min,. mm to mm from to min, mm to mm from to min; Methanol: % from to min, % to % from to min, % from to min, % to % Eluent: Sodium hydroxide: mm from to min, mm to mm from to min, mm to mm from to min Methanol: % from to min, % to % from to min, % from to min, % to % from to min from to min Flow Rate:. ml/min Flow Rate:. ml/min Temperature: C Temperature: C Inj. Volume: µl Inj. Volume: µl Dionex ASRS-ULTRA mm, Dionex ASRS-ULTRA mm, A,, B,, Figure. Effect of solvent on Dionex IonPac AS and AS-HC selectivity. Increased Flexibility for Methods Development The solvent compatibility of the Dionex IonPac AS and AS-HC columns permits the use of HPLC organic solvents in the eluent to modify ion- exchange selectivity. Both hydroxide concentration gradients and organic solvent gradients combined with elevated temperature can be used to achieve optimum resolution of closely eluting analyte pairs. The retention of the B A & C. Quinate. mg/l (ppm). Fluoride.. Lactate.. Acetate.. Glycolate.. Propionate.. Formate.. Butyrate.. Pyruvate.. Valerate.. Monochloroacetate.. Galacturonate.. Bromate.. Chloride.. Nitrite.. Sorbate.. Trifluoroacetate.. Bromide.. Nitrate.. Glutarate.. Succinate.. Maleate.. Malonate.. Carbonate. Tartrate.. Maleate.. Fumarate.. Sulfate.. Oxalate.. Ketomalonate. Tungstate. Phosphate. Citrate. cis-aconitate. trans-aconitate more hydrophobic member of the unresolved pair is decreased more by the addition of solvent, which improves resolution. Figure illustrates the effect of solvent on selectivity of monovalent and divalent organic acids on the Dionex IonPac AS and AS-HC columns. The columns can be operated at ambient or elevated temperatures. The use of a controlled column temperature ensures reproducible results.

5 Dionex IonPac AS-HC for Monovalent and Divalent Organic Anions The Dionex IonPac AS-HC column provides improved separation of monovalent and divalent organic acids and inorganic anions using a sodium or potassium hydroxide gradient at a controlled temperature of ºC. Because higher eluent concentrations can be used to separate monovalent anions, the higher capacity allows injection of more concentrated samples without overloading and provides improved peak shapes for monovalent organic acids in complex matrices, as illustrated in Figure. Column: Dionex IonPac AG-HC, AS-HC, mm Eluent: Sodium hydroxide: mm from to min, mm to mm from to min, mm to mm from to min, mm to mm from to min, mm to mm from to min, mm to mm from to min Flow Rate:. ml/min Temperature: C Inj. Volume:. ml Dionex ASRS-ULTRA mm,. Fluoride. µg/l (ppb). Acetate.. Formate.. Chloride.. Nitrite.. Bromide.. Nitrate.. Carbonate. Sulfate.. Oxalate.. Phosphate. IonPac AS-HC for Trace Inorganic and Organic Acid Anions Using a Large-Loop Injection The high-capacity Dionex IonPac AS-HC allows large injections of high ionic strength samples without loss of peak efficiencies and eliminates the need for sample dilution. High capacity allows for the determination of trace inorganic anions and organic acids using a large- loop injection. Figure illustrates the sepa ration of inorganic anions and organic acids using a large-loop injection with a sodium hydroxide gradient at a controlled temperature of ºC. Low ppb levels of these analytes can easily be determined using a. ml injection loop on a mm microbore system. Determination of Carboxylic Acids in Complex Matrices The Dionex IonPac AS-HC column can be used to monitor the organic acid and inorganic anion content in the quality control of foods, wines, and beverages. The high capacity of the column makes it ideal for the analysis of organic acids and inorganic anions in complex matrices. Samples high in ionic strength can be injected directly onto the Dionex IonPac AS-HC and do not cause overloading problems or poor peak shapes for monovalent organic acids (e.g., lactate, acetate, propionate, formate, and butyrate). Figure compares the Dionex IonPac AS and the AS-HC columns for the analysis of beer. The Dionex IonPac AS-HC column shows improved peak shapes for the monovalent organic acids. Butyrate is an important indicator of deterioration of foods and beverages and can be easily separated from formate on the Dionex IonPac AS-HC column with a sodium hydroxide gradient/ methanol gradient step change. Figure. Separation of inorganic anions and organic acids using a large-loop injection on the microbore ( mm) Dionex IonPac AS-HC column. Column A: Eluent: Dionex IonPac AG, AS, mm Column B: Sodium hydroxide:. mm from Eluent: to min,. mm to mm from to min, mm to mm from to min; Methanol: % from. to min. ml/min Flow Rate: Flow Rate: Temperature: C Sample Prep: Diluted :, treated with Thermo Scientific Dionex OnGuard-RP Inj. Volume: µl Detection: A Suppressed conductivity, Dionex ASRS-ULTRA mm, Dionex IonPac AG-HC, AS-HC, mm Sodium hydroxide: mm from to min, mm to mm from to min, mm to mm from to min, mm to mm from to min; Methanol: % from to min. ml/min Temperature: C Sample Prep: Diluted :, treated with Dionex OnGuard-RP Inj. Volume: µl Detection: B Suppressed conductivity, Dionex ASRS-ULTRA mm,. Lactate. Acetate. Formate. Butyrate ( ppm spike). Pyruvate. Chloride. Bromide. Nitrate. Succinate. Malate. Carbonate. Fumarate. Sulfate. Oxalate. Phosphate. Citrate. Isocitrate. cis-aconitate. trans-aconitate Figure. Comparison of the Dionex IonPac AS and AS-HC columns for the analysis of beer spiked with mg/l (ppm) butyrate.

6 Dionex IonPac AS Anion-Exchange Column Figures and illustrate the separation of organic acids and inorganic anions in red wine and apple juice using the Dionex IonPac AS-HC column. This column provides improved resolution over the AS column for components with widely varying concentration ratios. The Dionex IonPac AS-HC column resolves succinate and malate, even at very different concentrations, as shown in Figure. Dionex IonPac AS-HC for Organic Acids and Inorganic Anions in Fermentation Broths Monitoring fermentation broths is necessary for process control, because many ingredients resulting from metabolic processes affect yield or quality of the desired fermentation products. Inorganic anions maintain osmolarity and provide co-factors for biosynthesis. Certain organic acids are waste products, and their accumulation inhibits cell growth or product yields. The high capacity of the Dionex IonPac AS-HC column allows for the determination of a number of inorganic anions and organic acids in fermentation broths. Figure illustrates the separation of common fermentation broth anions using the Dionex IonPac AS-HC column with a sodium hydroxide gradient at a controlled temperature of C. Column: Dionex IonPac AG-HC, AS-HC, mm Eluent: Sodium hydroxide: mm from to min, mm to mm from to min, mm to mm from to min, mm to mm from to min; Methanol: % from to min Flow Rate:. ml/min Temperature: C Sample Prep: Diluted :, treated with Dionex OnGuard -RP Inj. Volume: µl Figure. Analysis of red wine using the Dionex IonPac AS-HC column. Unk Column: Dionex IonPac AG-HC, AS-HC, mm Eluent: Sodium hydroxide: mm from to min, mm to mm from to min, mm to mm from to min, mm to mm from to min; Methanol: % from to min Flow Rate:. ml/min Temperature: C Sample Prep: Diluted :, treated with OnGuard-RP Inj. Volume: µl Dionex ASRS-ULTRA mm,. Lactate. Acetate. Formate. Pyruvate. Galacturonate. Chloride. Nitrate. Succinate. Malate. Tartrate. Quinate. Lactate. Acetate. Formate. Galacturonate. Chloride. Succinate. Fumarate. Sulfate. Oxalate. Phosphate. Citrate. Isocitrate. cis-aconitate. trans-aconitate Unk = Unknown Dionex ASRS-ULTRA mm,. Malate. Sulfate. Oxalate. Phosphate. Citrate. Isocitrate. cis-aconitate Figure. Analysis of apple juice using the Dionex IonPac AS-HC column. Column: Dionex IonPac AG-HC, AS-HC, mm Eluent:. mm Sodium hydroxide gradient Flow Rate:. ml/min Inj. Volume: µl Temperature C Sample: mg/l each analyte Dionex ASRS-ULTRA mm, AutoSuppression recycle mode,,,. Lactate. Acetate. Propionate. Formate. -Keto-D-Gluconate. Pyruvate. Valerate. Monochloroacetate. Bromate. Chloride. Phenylacetate. Bromide. Nitrate. Malate. Methylmalonate. Carbonate. Malonate. Maleate. Sulfate. Oxalate. Phosphate. Citrate. Isocitrate. Pyrophosphate. Trichloroacetate A Figure. Separation of common fermentation broth anions using the Dionex IonPac AS-HC column.

7 Gradient Separations as Simple as Isocratic Runs with Eluent Generation The Eluent Generator produces high-purity potassium hydroxide eluent electrolytically, eliminating the need for eluent preparation. Only a source of deionized water is required. The potassium hydroxide eluent produced is free of carbonate contamination. The use of carbonate-free hydroxide eluents minimizes baseline shifts during hydroxide gradients which provides greater retention time reproducibility, lower background conductivity, and lower detection limits for target analytes. Figure illustrates the gradient separation of inorganic anions and organic acids in grape juice using a Dionex IonPac AS-HC using a potassium hydroxide gradient delivered by an Eluent Generator. Figure illustrates the use of the Dionex IonPac AS column with the Eluent Generator for an application of interest to the food industry, determination of sorbate, a sweetener used in cake frosting. In this example, a potassium hydroxide gradient is used to elute sorbate in less than min and also resolve other inorganic anions and organic acids present in cake frosting. Column: Eluent A: Eluent B: Gradient IonPac AG-HC, AS-HC, mm Potassium hydroxide Sodium hydroxide mm from to min, to mmat min, to mm at min; Methanol: % from min. ml/min Flow Rate: Inj. Volume: µl Temperature: C Sample Prep: Diluted :, treated with Dionex OnGuard-RP Inj. Volume: µl A EG Gradient B Conventional Gradient Dionex ASRS-ULTRA mm,. Quinate. mg/l (ppm). Malate.. Fluoride.. Malonate/. Lactate. Tartrate.. Galacturonate.. Maleate.. Chloride.. Sulfate.. Nitrate.. Oxalate.. Glutarate.. Phosphate.. Succinate.. Citrate.. Iso-Citrate. Figure. Analysis of grape juice on a Dionex IonPac AS-HC column using a potassium hydroxide gradient delivered by an Eluent Generator. Column: Dionex IonPac AG, AS, mm Eluent: Potassium hydroxide,. mm isocratic for. min, to mm at min, to mmat min Eluent Source: EG Flow Rate:. ml/min Inj. Volume: µl Dionex ASRS-ULTRA mm, AutoSuppression recycle mode Sample Prep: Diluted : with deionized water. Sorbate. mg/l (ppm) Figure. Analysis of sorbate in cake frosting using a Dionex IonPac AS column using a potassium hydroxide gradient delivered by an Eluent Generator.

8 Dionex IonPac AS Anion-Exchange Column Dionex IonPac AS for Anions in Hydrogen Peroxide Fabricators of electronic components use hydrogen peroxide during the manufacturing process. Monitoring chemical reagents for anionic impurities is important because the anionic contaminants can cause corrosion when present in manufacturing solutions. A method has been developed using the Dionex IonPac AS column to determine inorganic anions in stabilized hydrogen peroxide. Figure illustrates the separation of the anionic contaminants in hydrogen peroxide using a large-loop injection with a sodium hydroxide gradient. Dionex AS-HC for Trace Anions in Methanesulfonic Acid Monitoring anionic contaminants in chemical reagents is an important quality control function for specialty chemical manufacturers. The high-capacity Dionex IonPac AS-HC column is ideal for determining trace anions in concentrated acids. Figure illustrates the separation of trace chloride and sulfate in concentrated methanesulfonic acid using the Dionex IonPac AS-HC column. Chloride and sulfate can easily be determined down to the low ppb level with good peak shape even in the presence of high concentrations of methanesulfonic acid. Dionex AS Column for Polarizable Anions The Dionex AS column separates polarizable anions in complex matrices. As shown in Figure, for highly retained hydrophobic anions such as iodide, perchlorate, thiosulfate, and thiocyanate, retention times can be significantly decreased and peak efficiencies improved by using eluents containing organic solvents. This approach is ideal for the determination of iodide, perchlorate, thiocyanate, and thiosulfate. The Dionex IonPac AS column is recommended for this separation without the use of solvents in the eluent. S *Concentrations are of undiluted sample Column: Dionex IonPac AS, mm Concentrator Column: Dionex IonPac AG, mm Eluent:. mm Sodium hydroxide, min. mm Sodium hydroxide gradient, min Flow Rate:. ml/min Inj. Volume: µl Dionex ASRS-ULTRA mm, AutoSuppression recycle mode Sample: % Hydrogen peroxide (reagent grade, C), diluted : Peaks*:. Chloride. mg/l (ppm)*. Nitrate.. Sulfate.. Phosphate.. Pyrophosphate. Figure. Determination of anions in % stabilized hydrogen peroxide using the Dionex IonPac AS column. Column: Dionex IonPac AG-HC, AS-HC, mm Eluent: Sodium hydroxide: mm from to min, mm to mm from to min Flow Rate:. ml/min Sample Prep: Diluted : Inj. Volume: µl Dionex ASRS-ULTRA mm, AutoSuppression recycle mode. Fluoride µg/l (ppb). Unknown. Methanesulfonic acid. Chloride. Sulfate Figure. Dionex IonPac AS-HC for trace anions in concentrated methanesulfonic acid. Column: Dionex IonPac AS, mm Eluent: mm Sodium hydroxidein % Methanol Flow Rate:. ml/min Inj. Volume: µl Dionex ASRS-ULTRA mm,. Fluoride mg/l (ppm). Chloride. Nitrate. Sulfate. Phosphate. Iodide. Thiocyanate. Thiosulfate. Perchlorate Figure. Separation of common inorganic anions, including iodide, perchlorate, thiocyanate, and thiosulfate, using the Dionex IonPac AS column.

9 Dionex IonPac AS Column for Highly Charged Anions The determination of highly charged anions such as polyphosphates or polycarboxylates is possible using gradient elution on a mm or mm Dionex IonPac AS column. As shown in Figure, the high hydroxide selectivity on the Dionex IonPac AS resin permits elution of these ions at lower hydroxide concentrations than are possible on the Thermo Fisher Scientific OmniPac PAX-, permitting the use of either mm or mm columns. The Dionex IonPac AS column is ideal for the determination of polyphosphates, polycarboxylates, and polysulfonates. Column: Dionex IonPac AS, mm Eluent: Sodium hydroxide mm to mm in min Flow Rate:. ml/min ASRS-ULTRA mm, Dionex AutoSuppression recycle mode A Polyphosphate Standard. Chloride mg/l (ppm). Carbonate. Sulfate. Phosphate. Pyrophosphate. Trimetaphosphate. Tripolyphosphate. Tetrapolyphosphate. Tetrametaphosphate Unk = Unknown B Detergent Sample mg/l (ppm) Unk - Figure. Separation of polyphosphates in detergent. Table. Column selection table. Analyte Fast profiling of mono-, di-, and trivalent organic acids and inorganic anions in well-characterized samples High resolution of mono- and divalent organic acids Monovalent organic acids (lactate, acetate, propionate, formate, butyrate) Trace components in complex matrices using large loop injection Organic acids and inorganic anions in high ionic strength matrices Organic acids in high ionic strength matrices (inorganic anions elute in void) Aromatic acids with conductivity detection Polarizable anions (iodide, thiocyanate, thiosulfate, perchlorate) Polyphosphates, polycarboxylates, and polysulfonates Preservatives (benzoate, citrate, sorbate) Recommended Column AS AS-HC AS-HC AS-HC AS-HC ICE-AS or ICE-AS AS AS or AS AS or AS AS-HC

10 Dionex IonPac AS Anion-Exchange Column Dimensions Maximum Operating Pressure MPa ( psi) Mobile Phase Compatibility SPECIFICATIONS Dionex IonPac AS Analytical: mm and mm Dionex IonPac AG Guard: mm and mm Dionex IonPac AS-HC Analytical: x mm and x mm Dionex IonPac AG-HC Guard: x mm and x mm Dionex IonPac AS-HC Capillary:. x mm Dionex IonPac AS-HC Capillary Guard:. x mm ph ; % HPLC solvents Substrate Characteristics Dionex IonPac AS Bead diameter: µm Dionex IonPac AS-HC Bead diameter:. µm Dionex IonPac AS Pore size: Microporous, < Å Dionex IonPac AS-HC Pore size: Å Crosslinking: (%DVB): % Crosslinking: (%DVB): % Latex Characteristics Dionex IonPac AS Functional Group: Dionex IonPac AS-HC Functional Group: Alkanol quaternary ammonium ion Alkanol quaternary ammonium ion Latex Crosslinking: % Latex Crosslinking: % Latex Diameter: nm Latex Diameter: nm Hydrophobicity: Very Low Hydrophobicity: Medium Low Capacity Dionex IonPac AS Dionex IonPac AS-HC µeq ( mm Analytical). µeq ( mm Analytical). µeq ( mm Guard). µeq ( mm Guard) Column Construction Dionex IonPac AS Dionex IonPac AS-HC µeq ( mm Analytical) µeq ( mm Analytical) µeq ( mm Guard) µeq ( mm Guard). μeq (. mm Capillary Column). μeq (. mm Capillary Guard) PEEK with threaded ferrule-style end fittings. All components are nonmetallic. System Requirements The Dionex IonPac AS-HC Capillary Column is recommended for use with the Thermo Scientific Dionex ICS- or ICS- Capillary HPIC system. The Dionex IonPac AS and AS-HC Analytical Columns are recommended for use with the Dionex ICS- or ICS- RFIC system equipped with an eluent generator. The Dionex IonPac AS and AS-HC Analytical Columns can also be used with older Dionex IC systems equipped with an eluent generator or a Thermo Scientific Dionex RFC- Reagent-Free Controller. The eluent generator is used to automatically produce potassium hydroxide gradients from deionized water. Suppressor Recommendations For optimum ease of use and performance, the Dionex IonPac AS and AS-HC columns should be used with the Dionex ASRS Anion Self-Regenerating Suppressor or the Dionex ACES Anion Capillary Electrolytic Suppressor. Anion Trap Columns When using the eluent generator for eluent delivery, a Thermo Scientific Dionex CR-ATC Continuously Regenerated Anion Trap Column should be installed between the eluent generator cartridge (EGC) and the degas module. As an alternative for mm and mm systems, a Dionex IonPac ATC-HC column can be installed between the pump outlet and the EGC inlet. Alternatively, when using a manually-prepared sodium hydroxide gradient with the Dionex IonPac AS and AS-HC, the Dionex IonPac ATC- Anion Trap Column should be installed between the gradient pump and the injection valve to remove anionic contaminants from the eluent. Concentrator Columns For concentrator work with a mm or mm Dionex IonPac AS or AS-HC column, use the: Dionex IonPac AS or AS-HC guard column; Ultratrace Anion Concentrator Columns (Dionex IonPac UTAC-ULP, UTAC-XLP, UTAC-ULP, or UTAC-XLP ) or Trace Anion Concentrator Column (Dionex IonPac TAC-ULP) when a single piston pump such as the Thermo Scientific Dionex AXP pump (pulse damper required) is used for sample delivery. In addition to the concentrator columns listed above, use the Dionex IonPac UTAC-LP, UTAC-LP or TAC-LP when the sample is delivered using a syringe or a low-pressure autosampler, e.g., the Thermo Scientific Dionex AS-DV. For concentrator work with a. mm capillary column, use the Dionex IonPac AG-HC Capillary Guard Column or the Thermo Scientific Dionex IonSwift MAC- concentrator column.

11 Product Specifications ORDERING INFORMATION To order in the U.S., call ---, or contact the Thermo Fisher Scientific office nearest you. Outside the U.S., order through your local Thermo Fisher Scientific office or distributor. Refer to the following part numbers. Dionex IonPac AS Columns Part Number Dionex IonPac AS Analytical Column ( mm) Dionex IonPac AG Guard Column ( mm) Dionex IonPac AS Analytical Column ( mm) Dionex IonPac AG Guard Column ( mm) Dionex IonPac AS-HC Columns Part Number Dionex IonPac AS-HC Analytical Column ( mm) Dionex IonPac AG-HC Guard Column ( mm) Dionex IonPac AS-HC Analytical Column ( mm) Dionex IonPac AG-HC Guard Column ( mm) Dionex IonPac AS-HC Capillary Column (. mm) Dionex IonPac AG-HC Capillary Guard Column (. mm) Anion Trap Columns Part Number Dionex CR-ATC Continuously Regenerated Anion Trap Column (for use with systems equipped with an eluent generator or RFC- Reagent-Free Control) Dionex CR-ATC Continuously Regenerated Anion Trap Column (for use with capillary anion columns) Dionex IonPac ATC- Anion Trap Column ( mm) Dionex IonPac ATC- mm ( mm) Anion Trap Column (for use with mm columns) Dionex IonPac ATC- mm ( mm) Anion Trap Column (for use with mm columns) Dionex IonPac ATC-HC ( mm) Anion Trap Column (for use with the Dionex EG Eluent Generator) Trace Anion Concentrator Columns Part Number Dionex IonPac TAC- Trace Anion Concentrator ( mm) Dionex IonPac TAC-LP Trace Anion Concentrator ( mm) Dionex IonSwift MAC- Monolith Anion Concentrator (. mm) (for use with capillary IC) Dionex IonPac TAC-LP Trace Anion Concentrator ( mm) Dionex IonPac TAC-ULP Trace Anion Concentrator ( mm) Dionex IonPac UTAC-LP Ultra Trace Anion Concentrator Low Pressure ( mm Dionex IonPac UTAC-ULP Ultra Trace Anion Concentrator Ultra Low Pressure ( mm) Dionex IonPac UTAC-XLP Ultra Trace Anion Concentrator Extremely Low Pressure ( mm) Dionex IonPac UTAC-LP Ultra Trace Anion Concentrator Low Pressure ( mm) Dionex IonPac UTAC-ULP Ultra Trace Anion Concentrator Ultra Low Pressure ( mm) Dionex IonPac UTAC-XLP Ultra Trace Anion Concentrator Extremely Low Pressure ( mm) Thermo Fisher Scientific Inc. All rights reserved. PEEK is a trademark of Victrex PLC. All other trademarks are the property of Thermo Fisher Scientific Thermo Scientific Dionex products are Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales designed, developed, and manufactured representative for details. under an ISO Quality System. PS_E /S Dionex Products: Titan Way, PO Box, Sunnyvale, CA -, () - North America: U.S./Canada () - South America: Brazil () Europe: Austria (), Benelux () (), Denmark (), France (), Germany (), Ireland (), Italy (), Sweden (), Switzerland (), United Kingdom () Asia Pacific: Australia (), China (), India (), Japan (), Korea (), Singapore (), Taiwan ()