Size Exclusion Chromatography
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1 Springer Laboratory Size Exclusion Chromatography Bearbeitet von Sadao Mori, Howard G Barth 1. Auflage Buch. xiv, 234 S. Hardcover ISBN Format (B x L): 15,5 x 23,5 cm Gewicht: 1170 g Weitere Fachgebiete > Chemie, Biowissenschaften, Agrarwissenschaften > Analytische Chemie > Instrumentelle Chemische Analytik, Chromatographie schnell und portofrei erhältlich bei Die Online-Fachbuchhandlung beck-shop.de ist spezialisiert auf Fachbücher, insbesondere Recht, Steuern und Wirtschaft. Im Sortiment finden Sie alle Medien (Bücher, Zeitschriften, CDs, ebooks, etc.) aller Verlage. Ergänzt wird das Programm durch Services wie Neuerscheinungsdienst oder Zusammenstellungen von Büchern zu Sonderpreisen. Der Shop führt mehr als 8 Millionen Produkte.
2 Table of Contents 1 Introduction Molecular Weight Measurements Brief Description of Size Exclusion Chromatography Brief Historical Perspective Applications General References References Fundamental Concepts Separation Mechanism of SEC Geometric Models Thermodynamic Model Hydrodynamic Volume Concept SEC Parameters and Definitions Column and Calibration Curve Characteristics Peak Broadening Column Resolution References Instrumentation SEC Systems Used at Room Temperature High-Temperature SEC Systems Pumps and Injectors Pumps Injectors Detectors Introduction Concentration-Based Detectors Structure-Selective Detectors MW-Sensitive Detectors Columns Introduction Types of Packing Materials
3 VIII Table of Contents 4.3 Non-aqueous SEC Columns Aqueous SEC Columns Care and Handling SEC Method Development Sample Preparation Polymer Solubility Sample Solutions Sample Concentration Effects of Concentration Influence of Concentration Dependence on MW Calculation Calibration Curves Calibration of Columns Shape of the Calibration Curve Column Selection Non-aqueous SEC Columns Aqueous SEC Columns Mobile Phase Selection Non-aqueous SEC Aqueous SEC Preparation of the Mobile Phase Operating Conditions Injection Volume Flow Rate Column Temperature References Molecular Weight Averages and Distributions Calculation ofmw Averages MW Equations Calculation Procedure Reproducibility of Results Calculation ofmw Distribution Equations Calculation Procedure Automated Data Handling Band Broadening Corrections Band Broadening Equations Deconvolution Approaches Correction of MW Averages References
4 Table of Contents IX 7 Approaches to Molecular Weight Calibration Calibration with Monodisperse Standards Calibration with Polydisperse Standards Assumption of a Calibration Curve or MWD Use of Primary and Secondary Standards MW vs. Molecular Size Terminology of Molecular Size Parameters for Molecular Size Comparison of Size Parameters Extended Chain Length Extended Chain Structure Q-Factor Universal Calibration Intrinsic Viscosity Universal Calibration Curve Transformation of the Universal Calibration Curve Direct Calculation of MW with Universal Calibration Use of Secondary Standard(s) with Universal Calibration References Molecular-Weight-Sensitive Detectors Light Scattering Theory Instrumentation Methodology Problems of Refractive Index Viscometry Theory Instrumentation Methodology Combination with a Concentration Detector Methodology Selected Applications References Synthetic Polymers Separation of Oligomers Refractive Index and MW Retention Volume and MW Epoxy Resins Phenol-Formaldehyde Resins
5 X Table of Contents Melamine Resins Miscellaneous Polymers with Commonly Used Solvents Polymer/Solvent Combinations Poly(vinyl chloride) Polyorganophosphazene Cellulose Derivatives Polymers Requiring Special Solvents Polyamides Poly(ethylene terephthalate) Other Polymers Selected Topics Combinations of K and a Calculation of Detector Lag Time Comparison of Chromatograms References High-Temperature Size Exclusion Chromatography General Procedures for Preparation of Sample Solutions Selected Applications Polyethylene Branched Polyethylene Polypropylene Copolymers of Polyethylene High-Temperature-Resistant Polymers References Aqueous Size Exclusion Chromatography Non-Size Exclusion Effects Thermodynamics Types of Non-Size Exclusion Effects Elution Behavior of Water-Soluble Polymers Mobile Phase and Packing Selection Nonionic Polymers Anionic Polymers Cationic Polymers Proteins and Peptides Selected Applications Synthetic Polymers Biopolymers Polysaccharides References
6 Table of Contents XI 12 Special Applications Copolymer Analysis Introduction Calculation of MW Chemical Heterogeneity Preparative and Recycle SEC Preparative SEC Recycle SEC Separation of Small Molecules Selection of Experimental Conditions Additives in Polymers Application as a Cleanup Tool for Residue Analysis References Appendix I Appendix II Appendix III Appendix IV Subject Index
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