AN HPLC COLUMN InertSustain C1 AN HPLC COLUMN InertSustain C1 Inertsil continues to evolve to InertSustain Physical Properties
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1 AN HPLC COLUMN TM InertSustain C8 AN HPLC COLUMN InertSustain TM C8 Inertsil continues to evolve to InertSustain Physical Properties Silica : Newly Developed Silica Gel Particle Size : m, m, m Surface Area : 0 m /g Pore Size : 00 Å Pore Volume : 0.8 ml/g Bonded Phase : Octadecyl Groups End-capping : Complete Carbon Loading : % USP Code : L ph Range : 0
2 Inertness and Durability combined in a new HPLC column TM InertSustain C8 Generally, silica based columns are mechanically stable and provide high efficiencies, however, they cannot be used under alkaline conditions and their residual silanol groups tend to adsorb organic bases. InertSustain C8 employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise control of the silica properties. InertSustain C8 inherits the advantages of all the current Inertsil HPLC columns (e.g., extremely low operating back pressure, superior inertness to typically any analytes, high efficiency and compatibility with a wide range of solvents), but now can be used for wide ph analysis with consistent performance from column to column and lot to lot. st Stage [ Synthesi sis of Evolved Surface Silica] InertSustain C8 features Unmatched Inertness and Durability nd Stage Radically New Silica Gel It is not possible to end-cap 00 % of residual silanols using traditional chemical modification procedures. GL Sciences studied the possibility of developing a radically new type of silica, a silica that would provide both high inertness (base deactivation) and durability at a wide range of ph. st Stage [Synthesis of Evolved Surface Silica ] As the adsorption sites and silanol distribution are strictly controlled from the synthesis procedure of the Evolved Surface Silica, InertSustain C8 delivers unmatched inertness to virtually any type of analyte with high durability to acidic, basic mobile phase conditions. nd Stage [Chemical Bonding of ODS group] The introduction of Evolved Surface Silica and our cutting-edge chemical bonding technology make InertSustain C8 compatible with 00 % aqueous mobile phases, while maintaining strong non-polar retentivity. rd Stage [Complete End-capping] GL Sciences complete end-capping technology allows InertSustain C8 to provide high efficiency and superior peak shape even to those well-known strong adsorptive analytes.
3 TM InertSustain tain C8 Surface of Evolved Surface Silica st Stage Evolved Surface Silica InertSustain C8 employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise control of the silica properties and adsorption sites and silanol distribution are strictly controlled. rd Stage [ Complete Endd-capping] [ Chemi mical Bondi ding of ODS group] BENEFITS Introducing a Radically New Type of Silica The introduction of Evolved Surface Silica leads to efficient method development at a wide range of ph, creating the possibility of unique selectivity changes. Superior Peak Shape with Better Resolution As the adsorption sites and silanol distribution are strictly controlled on the Evolved Surface Silica, InertSustain C8 delivers highly stable chromatograms for qualitative and quantitative analysis for all compounds. Wide ph Compatibility and High Durability InertSustain C8 is highly durable to acidic and basic mobile phases and can endure from ph to 0. Some samples require high ph for dissolution or to maintain stability. InertSustain C8 would be the first choice column for such applications. Strict Quality Control Program results in High Reproducibility Since the entire manufacturing process is under the control of our factory in Japan,reliable and consistent performance from column to column and lot to lot can be stably supplied. Unmatched Operating Back Pressure Due to the low operating back pressure that InertSustain C8 offers, it can still deliver low pressure even using high-viscosity solvents. InertSustain C8 Other Brands C8
4 Quality and Performance of TM InertSustain Tailing of peaks or adsorption of peaks can be experienced when a column has residual adsorption sites. Such phenomenon would not be observed on InertSustain as the silanols are completely end-capped, having a neutral silica surface. To maintain precise product reproducibility, strict chromatographic tests for inertness, durability, theoretical plates and reproducibility of retention time are employed. Basic Compound Berberine chloride and Dextromethorphan is a strong basic compound. Severe tailing can be confirmed when the packing material contains residual silanol groups. As shown on the right, InertSustain delivers highly stable chromatograms for qualitative and quantitative analysis. Column Size : m 0. mm I.D. Eluent : CHCN / mm KHPO (ph 7.0, KHPO) = 0 / 70, v / v Flow Rate : 0. ml / min Sample: Detection : UV 0 nm.uracil.pyridine.phenol.berberine chloride.dextromethorphan InertSustain C8 Berberine chloride Dextromethorphan L-column ODS Luna C8 () XSelect CSH C8 CAPCELL PAK C8 MGlll.No Peak Acidic Compound Tailing will be observed on Brilliant Blue FCF and Salicylic acid when the surface of the packing material is basic. InertSustain provides superior peak shape as it has a neutral silica surface. Column Size : m 0. mm I.D. Eluent : CHCN / 0. %HPO = / 7, v / v Flow Rate : 0. ml / min Detection : UV nm Sample:.Brilliant Blue FCF.Phenol.Salicylic acid InertSustain C8 Brilliant Blue FCF L-column ODS Luna C8 () XSelect CSH C8 CAPCELL PAK C8 MGlll.No Peak.No Peak
5 TM InertSustain tain C8 Wide ph compatibility with Long Column Lifetime As shown in the experiment on the right, due to the introduction of Evolved Surface Silica, InertSustain C8 maintained high efficiency and peak shape for 00 hours while other wide ph column brands failed. Purging Column size : m, 0.6 mm I.D. Eluent : 0 mm Triethylamine (ph0.0) /CHOH = 70 / 0, v / v Flow Rate :.0 ml / min Col. Temp. : 0 C Analytical Eluent : CHCN / HO = 6 /, v / v Flow Rate :.0 ml / min Detection : UV nm Sample : Naphthalene Reliable Reproducibility, Performance and Quality Rigorous quality control of physical properties and strict chromatographic tests for inertness and selectivity, contribute to the production of InertSustain C8 with an outstanding reproducibility and long column lifetime. % Initial N Hours of Purging (h) Longer Column Lifetime! InertSustain C8 XTerra MS C8 (Waters) Gemini-NX C8 (Phenomenex) Selectivity Test 6 7 Strong Basic Compound Test Superior peak shape with highly reproducible chromatograms can be obtained even for those strong basic compounds Column : InertSustain C8 ( m 0.6 mm I.D.) Eluent : CHOH / HO=80 / 0, v / v Sample: Flow Rate : 0. ml / min. Uracil. Caffeine. Phenol. Butylbenzene. o-terphenyl Detection : UV nm 6. Amylbenzene 7. Triphenylene Column : InertSustain C8 ( m, 0.6 mm I.D.) Eluent : CHCN / mm KHPO (ph 7.0, KHPO ) = 0 / 60, v / v Flow Rate :.0 ml / min Detection : UV 0 nm Sample :. Dextromethorphan Extremely Low Operating Back Pressure Due to the introduction of Evolved Surface Silica, InertSustain C8 offers a significantly lower operating back pressure without sacrificing efficiency. As shown on the right, InertSustain C8 produces the lowest back pressure than other brands columns in the industry. Pressure (MPa) InertSustain C8 Zorbax Eclipse plus C8 Luna C8 () Hypersil GOLD C8 SunFire C8 Symmetry C8 Atlantis T C8 YMC Pro C8 Column size : m, 0.6 mm I.D. Eluent : CHOH / HO = 80 / 0, v / v Flow Rate :.0 ml / min
6 Applications Organic Acids Compatible with 00 % aqueous mobile phases Basic Drugs Wide ph Compatibility Intensity, cps 7.0e 6.0e.0e.0e.0e.0e.0e Intensity, cps 7.0e 6.0e.0e.0e.0e.0e.0e System : GL-700 HPLC system Column : InertSustain C8 ( m, 0.6 mm I.D.) Eluent : 0 mm NHHPO (ph.6, HPO) Flow Rate :.0 ml / min Detection : UV 0 nm Injection Vol. : 0 L Sample:.Oxalic acid ( 00 mg / L).Tartaric acid (000 mg / L).Glycolic acid (000 mg / L).Malonic acid (000 mg / L).Lactic acid (000 mg / L) 6.Acetic acid (000 mg / L) 7.Fumalic acid ( 0 mg / L) 8.Succinic acid (000 mg / L) 9.Acrylic acid ( 00 mg / L) ) 0. % HCOOH in HO(pH.) ) 0. % Ammonia solution in HO(pH 9.) System : LC800 HPLC system 000 Q TRAP Column : InertSustain C8 ( m, 7. mm I.D.) Eluent : ) CHCN / 0. % HCOOH in HO(pH.) = 0 / 90, v / v : ) CHCN / 0. % Ammonia solution(8 %) in HO(pH 9.) = 0 / 70, v / v Flow Rate : 0. ml / min Sample: Detection : LC / MS / MS (ESI, Positive, MRM). Ranitidine (00 ng / ml) Q:. Q :.0 Injection Vol. : L. Sulpiride (00 ng / ml) Q:. Q :. Antidepressants Effective for High Sensitivity analysis by LC/MS/MS Intensity, cps 9.00e 8.00e 7.00e 6.00e.00e 8.00e.00e 6.00e e Time, min System : LC800 HPLC system 000 Q TRAP Column : InertSustain C8 ( m, 0. mm I.D.) Eluent : A) 0. % HCOOH in CHCN : B) 0. % HCOOH in HO : A / B = / min - 0 / min - 0 / 60,v / v Flow Rate : 0. ml / min Detection : LC / MS / MS (ESI, Positive, MRM) Injection Vol. : L Sample : (Q / Q) (Q / Q). Sulpilide ( / ) 9. Nortriptyline (6 / ). Milnacipran (7 / 0) 0. Maprotiline (78 / 0). Trazodone (7 / 76). Amitriptyline (78 / ). Amoxapine ( / 7). Trimmipramine (9 / 00). Doxepin (80 / 07). Fluoxetine (0 / ) 6. Desipramine (67 / 7). Sertraline (06 / 7) 7. Fluvoxamine (9 / 7). Clomipramine ( / 86) 8. Imipramine (8 / 86) (each 00 ng / ml) Glycyrrhizins Effective for Herbal Medicine samples Anthocyanins in Bilberry Effective for Natural Plant Pigments , System : GL-700 HPLC System Column : InertSustain C8 ( m, 0.6 mm I.D.) Eluent : A) CHCN B). % CHCOOH A / B = 0 / 60, v / v Flow Rate : ml / min Detection : UV nm Injection Vol. : 0 L Sample :. Glycyrrhizic acid dipotassium salt (0 mg / L). Propyl p-hydroxybenzoate (0 mg / L) System : LC800 HPLC system Column : InertSustain C8 ( m, 0.6 mm I.D.) Eluent : A) HO / HCOOH =90 / 0 B) HO / CHCN / CHOH / HCOOH = 0 /. /. / 0 A / B = 9 / 7 - min - 7 / -0 min - / 6 - min - 0 / 00 - min - 0 / 00,v / v Flow Rate :.0 ml / min Col. Temp. : 0 C Detection : VIS nm Injection Vol. : 0 L Sample : Bilberry powder (. mg/ml). Delphinidin--O-galactoside 9. Peonidin--O-galactoside. Delphinidin--O-glucoside 0. Petunidin--O-arabinoside. Cyanidin--O-galactoside. Peonidin--O-glucoside. Delphinidin--O-arabinoside. Malvidin--O-galactoside. Cyanidin--O-glucoside. Peonidin--O-arabinoside 6. Petunidin--O-galactoside. Malvidin--O-glucoside 7. Cyanidin--O-grabinoside. Malvidin--O-arabinoside 8. Petunidin--O-glucoside
7 TM InertSustain tain C8 Analytical Columns Particle Size: m Max. Operating Pressure: 80 MPa (800 Bar) Particle Size: m Max. Operating Pressure: 0 MPa (00 Bar) HP Series Particle Size: m Max. Operating Pressure: 0 MPa (00 Bar) Particle Size: m Max. Operating Pressure: 0 MPa (00 Bar) I.D. (mm)..0 Length (mm) Cat.No. Cat.No I.D. (mm).0. Length (mm) Cat.No. Cat.No I.D. (mm) Length (mm) Cat.No. Cat.No. Cat.No. Cat.No I.D. (mm)..0.6 Length (mm) Cat.No. Cat.No. Cat.No I.D. (mm).0. Length (mm) Cat.No. Cat.No I.D. (mm) Length (mm) Cat.No. Cat.No. Cat.No. Cat.No
8 Conventional Guard Columns, Cartridge Guard Columns Diameter of the Analytical Column Applicable (mm) Conventional Guard Column Length (mm) I.D. (mm) Replacement Cartridge E Guard Column ( EA.) Particle Size Cartridge E Holder / Cartridge Set Cartridge E Guard Columns and Holder Particle Size , , , , , Preparative Columns Particle Size: m I.D. (mm) Length (mm) Cat.No. Cat.No. Cat.No. Cat.No Preparative Guard Columns Particle Size: m I.D. (mm) x Length (mm) Cat.No * End-fittings are /6 Waters compatible. * Other column sizes available upon request. Worldwide Ordering Information To find your local distributor, please visit our website at Simply select your country from the list and your local distributor information will be displayed. * All trademarks are the property of their respective owners. We reserve the right to change specifications to make improvements without notice. Distributor: - Nishishinjuku 6-chome, Shinjuku-ku, Tokyo 6-0. Japan Tel Fax world@gls.co.jp 000ABT
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