CLIPEUS - A Small Shield for a Big Job
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- Tamsyn Farmer
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1 CLIPEUS - A Small Shield Virgil s Aeneid gave us a sanguine account of the problems encountered when working without a Clipeus a small shield popularized by the Romans. While arguably a less painful experience, chromatographers 2,000 years later still struggle when they work with inadequately shielded HPLC columns that are the by product of flawed end-capping technology. Higgins Analytical places a great deal of emphasis on effective silanol end-capping techniques. The bulky size and secondary chemical functionality of the end-capping moiety on TARGA (a large shield) HPLC columns and cartridges make them popular choices for researchers developing powerful LC/MS techniques or chromatographers doing routine analysis of difficult polar compounds. CLIPEUS - A Small Shield for a Big Job Phases C8, C18, Cyano, Phenyl Particle Sizes 4.5µm Pore Size 120Å Pore Volume 0.8mL/gm Surface Area 330m 2 /gm %Carbon C18 = 18% (w/w) Phase type Extremely well end-capped monofunctional phases Silica Class Type B Hardware Stainless steel and PEEK columns and cartridges: Nanobore columns Page 6 Microbore columns Page 6 Analytical and Narrowbore columns Page 7 Semi-Prep and Prep Columns Page 7 Cartridge columns - Analytical Page 8 Cartridge Columns - LC/MS Page 8 High Speed 10mm Semi-Prep cartridges Page 9 Prep Guard Cartridges Page 9 Analytical Guard Cartridges Page 9 Narrowbore guards and LC/MS Cartridges Page 9 Our CLIPEUS columns are based on the same ultra-pure, Type B silica gel we use for our TARGA products, but the end capping is done with the more traditional methodology employing TMS, a small molecule; hence CLIPEUS - a small shield. CLIPEUS columns have Higgins Analytical s characteristically high lot-to-lot reproducibility because our carefully controlled reaction of monofunctional silanes can be driven to completion in a reproducible manner. This unbuffered Pyridine and Phenol chromatogram illustrates the absence of any undesirable solute/silanol interaction on a CLIPEUS C18 HPLC Column. Applications The high purity base silica and thoroughly end capped bonded phases insure that CLIPEUS columns and cartridges will perform well in demanding situations where many other column brands fail. Even polar compounds elute as sharp and symmetrical peaks permitting surprisingly short columns to be used for high speed analysis. In addition to C8 and C18 phases, CLIPEUS columns are available with phenyl or cyano functionality. CLIPEUS C18 5µm 150x4.6mm 40% MeOH/water (unbuffered) 1mL/min, 25ºC, 254nm P/N HC185-CS1546
2 CLIPEUS CLIPEUS Offers a Wide Range of Hydrophobicity The highly pure silica backbone and the thorough end-capping process that are characteristics of CLIPEUS HPLC columns enable a wide choice of column selectivity without compromising chromatographic performance. Not only do the columns with relatively small cyano and phenyl functional groups perform as efficiently as do the longer chain C8 and C18 versions of CLIPEUS, they also produce symmetrical peaks for acidic and basic analytes. This is usually not the case for these types of columns from most HPLC column manufacturers. The four CLIPEUS chemistry offerings and the wide variety of column and cartridge formats, with internal diameters ranging more than two orders of magnitude, present a powerful selection to a chromatographer desiring to optimize a difficult separation or to maximize the speed of a high throughput application. Comparative Selectivity of CLIPEUS HPLC Columns Neutrals Test Mix NIST Test Mix CLIPEUS C18 150x4.6mm P/N HC185-CS1546 CLIPEUS C8 150x4.6mm P/N HC085-CS CLIPEUS Phenyl 150x4.6mm P/N HPHN5-CS CLIPEUS Cyano 150x4.6mm P/N HCNP5-CS1546 Carbamates and Urea Pesticides EPA 632A Industrial and Municipal Waste Water Column P/N HC185-CS Monuron 2. Propoxur 3. Carbaryl 4. Propham 5.Diuron 6. Linuron 7. Methiocarb 8. Mexacarbate 9. Chlorpropham 10. Barban 11. Neburon 50% MeCN/water, 1.5mL/min, 205nm 80% MeOH/Buffer, 1.5mL/min, 1. Thiourea 2. Phenol 3. Acetophenone 4. Nitrobenzene 5. Toluene 6. Napthalene No Metal Activity nor Residual Silanols The data in the chromatograms above clearly illustrate our purity and endcapping claims for the CLIPEUS 254nm Buffer: 20mM potassium phosphate 1. Uracil 2. Toluene 3. Ethylbenzene 4. Quinizarin 5. Amitriptyline family of HPLC columns. Quinizarin, a strong metal chelator, and amitriptyline, a very sensitive solute for residual silanols, both elute as very symmetrical peaks on all four column types making CLIPEUS a reliable choice for even difficult analytes.
3 Nanobore Columns CLIPEUS C8 5µm 150µm I.D. HC085-CN0315 $300 HC085-CN0515 $300 HC085-CN1015 $325 CLIPEUS C18 5µm 150µm I.D. HC185-CN0315 $300 HC185-CN0515 $300 HC185-CN1015 $325 CLIPEUS Cyano 5µm150µm I.D. HCNP5-CN0315 $315 HCNP5-CN0515 $315 HCNP5-CN1015 $365 CLIPEUS Phenyl 5µm150µm I.D. HPHN5-CN0315 $315 HPHN5-CN0515 $315 HPHN5-CN1015 $365 Microbore Columns CLIPEUS C8 5µm 1.0mm - 0.5mm - 0.3mm I.D. HC085-CS0301 $290 HC085-CS0501 $300 HC085-CS1001 $325 HC085-CS1501 $350 HC085-CS2501 $380 HC085-CS03M5 $290 HC085-CS05M5 $300 HC085-CS10M5 $325 HC085-CS15M5 $350 HC085-CS03M3 $290 HC085-CS05M3 $300 HC085-CS10M3 $325 CLIPEUS C18 5µm 1.0mm - 0.5mm - 0.3mm I.D. HC185-CS0301 $290 HC185-CS0501 $300 HC185-CS1001 $325 HC185-CS1501 $350 HC185-CS2501 $380 HC185-CS03M5 $290 HC185-CS05M5 $300 HC185-CS10M5 $325 HC185-CS15M5 $350 HC185-CS03M3 $290 HC185-CS05M3 $300 HC185-CS10M3 $325 CLIPEUS Cyano 5µm 1.0mm - 0.5mm - 0.3mm I.D. HCNP5-CS0301 $305 HCNP5-CS0501 $315 HCNP5-CS1001 $340 HCNP5-CS1501 $365 HCNP5-CS2501 $395 HCNP5-CS03M5$305 HCNP5-CS05M5$315 HCNP5-CS10M5 $340 HCNP5-CS15M5 $365 HCNP5-CS03M3$305 HCNP5-CS05M3$315 HCNP5-CS10M3 $340 CLIPEUS Phenyl 5µm 1.0mm - 0.5mm - 0.3mm I.D. HPHN5-CS0301 $305 HPHN5-CS0501 $315 HPHN5-CS1001 $340 HPHN5-CS1501 $365 HPHN5-CS2501 $395 HPHN5-CS03M5$305 HPHN5-CS05M5$315 HPHN5-CS10M5 $340 HPHN5-CS15M5 $365 HPHN5-CS03M3$305 HPHN5-CS05M3$315 HPHN5-CS10M3 $340
4 CLIPEUS Ordering Information Analytical Columns - Stainless Steel CLIPEUS C8 5µm 4.6, 3.0, and 2.1mm I.D. s.s. columns HC085-CS0346 $250 HC085-CS0546 $260 HC085-CS1046 $270 HC085-CS1546 $305 HC085-CS2546 $325 HC085-CS0330 $250 HC085-CS0530 $260 HC085-CS1030 $270 HC085-CS1530 $305 HC085-CS2530 $325 HC085-CS0321 $250 HC085-CS0521 $260 HC085-CS1021 $270 HC085-CS1521 $305 HC085-CS2521 $325 CLIPEUS C18 10µm 4.6mm I.D. s.s. columns HC181-CS0346 $230 HC181-CS0546 $240 HC181-CS1046 $250 HC181-CS1546 $275 HC181-CS2546 $300 CLIPEUS C18 5µm 4.6, 3.0, and 2.1mm I.D. s.s. columns HC185-CS0346 $250 HC185-CS0546 $260 HC185-CS1046 $270 HC185-CS1546 $305 HC185-CS2546 $325 HC185-CS0330 $250 HC185-CS0530 $260 HC185-CS1030 $270 HC185-CS1530 $305 HC185-CS2530 $325 HC185-CS0321 $250 HC185-CS0521 $260 HC185-CS1021 $270 HC185-CS1521 $305 HC185-CS2521 $325 CLIPEUS Cyano 5µm 4.6, 3.0, and 2.1mm I.D. s.s. columns HCNP5-CS0346 $260 HCNP5-CS0546 $270 HCNP5-CS1046 $280 HCNP5-CS1546 $305 HCNP5-CS2546 $325 HCNP5-CS0330 $260 HCNP5-CS0530 $270 HCNP5-CS1030 $280 HCNP5-CS1530 $305 HCNP5-CS2530 $325 HCNP5-CS0321 $260 HCNP5-CS0521 $270 HCNP5-CS1021 $280 HCNP5-CS1521 $305 HCNP5-CS2521 $325 CLIPEUS Phenyl 5µm 4.6, 3.0, and 2.1mm I.D. s.s. columns HPHN5-CS0346 $260 HPHN5-CS0546 $270 HPHN5-CS1046 $280 HPHN5-CS1546 $320 HPHN5-CS2546 $340 HPHN5-CS0330 $260 HPHN5-CS0530 $270 HPHN5-CS1030 $280 HPHN5-CS1530 $320 HPHN5-CS2530 $340 HPHN5-CS0321 $260 HPHN5-CS0521 $270 HPHN5-CS1021 $280 HPHN5-CS1521 $320 HPHN5-CS2521 $340 Semi-Prep and Prep Columns CLIPEUS C8 5µm CLIPEUS C18 10µm CLIPEUS C18 5µm 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 150x10mm 250x10mm HC085-CS0510 $440 HC085-CS1010 $550 HC085-CS1510 $800 HC085-CS2510$ x20mm 100x20mm 150x20mm 250x20mm HC085-CS0520$1120 HC085-CS1020 $1400 HC085-CS1520$1850 HC085-CS2520$ and 20mm I.D. s.s. columns 50x10mm 100x10mm 150x10mm 250x10mm HC181-CS0510 $300 HC181-CS1010 $370 HC181-CS1510 $530 HC181-CS2510 $670 50x20mm 100x20mm 150x20mm 250x20mm HC181-CS0520 $755 HC181-CS1020 $945 HC181-CS1520$1230 HC181-CS2520$ and 20mm I.D. s.s. columns 50x10mm 100x10mm 150x10mm 250x10mm HC185-CS0510 $440 HC185-CS1010 $550 HC185-CS1510 $800 HC185-CS2510$ x20mm 100x20mm 150x20mm 250x20mm HC185-CS0520$1120 HC185-CS1020 $1400 HC185-CS1520$1850 HC185-CS2520$2400
5 See Pages for information about holders required for these cartridge columns. HAICART - Stainless Steel Analytical Cartridge Columns CLIPEUS C8 5µm 4.6 and 3.2mm I.D. s.s. Analytical Cartridges HC085-CF0446 $270 HC085-CF0546 $285 HC085-CF1046 $270 HC085-CF0432 $270 HC085-CF0532 $285 HC085-CF1032 $270 CLIPEUS C18 5µm 4.6 and 3.2mm I.D. s.s. Analytical Cartridges HC185-CF0446 $270 HC185-CF0546 $285 HC185-CF1046 $270 HC185-CF0432 $270 HC185-CF0532 $285 HC185-CF1032 $270 CLIPEUS Cyano 5µm 4.6 and 3.2mm I.D. s.s. Analytical Cartridges HCNP5-CF0446 $285 HCNP5-CF0546 $300 HCNP5-CF1046 $295 HCNP5-CF0432$2850 HCNP5-CF0532 $300 HCNP5-CF1032 $295 CLIPEUS Phenyl 5µm 4.6 and 3.2mm I.D. s.s. Analytical Cartridges HPHN5-CF0446 $285 HPHN5-CF0546 $300 HPHN5-CF1046 $295 HPHN5-CF0432 $285 HPHN5-CF0532 $300 HPHN5-CF1032 $295 HAIPEEK - PEEK Analytical Cartridge Columns CLIPEUS C8 5µm CLIPEUS C18 5µm CLIPEUS Cyano 5µm CLIPEUS Phenyl 5µm HC085-CK0421 $250 HC085-CK0521 $255 HC185-CK0421 $250 HC185-CK0521 $255 HCNP5 -CK0421$265 HCNP5-CK0521 $270 HPHN5-CK0421 $265 HPHN5-CK0521 $270
6 CLIPEUS Ordering Information HAISpeed10-10mm I.D. Stainless Steel Cartridge Columns CLIPEUS C8 5µm 10mm I.D. s.s. Analytical / Semi-Prep Cartridges HC085-CF0310 $250 HC085-CF0510 $295 HC085-CF1010 $370 CLIPEUS C18 10µm 10mm I.D. s.s. Analytical / Semi-Prep Cartridges HC181-CF0310 $170 HC181-CF0510 $200 HC181-CF1010 $250 CLIPEUS C18 5µm 10mm I.D. s.s. Analytical / Semi-Prep Cartridges HC185-CF0310 $250 HC185-CF0510 $295 HC185-CF1010 $370 CLIPEUS Cyano 5µm 10mm I.D. s.s. Analytical / Semi-Prep Cartridges HCNP5-CF0310 $265 HCNP5-CF0510 $310 HCNP5-CF1010 $385 CLIPEUS Phenyl 5µm 10mm I.D. s.s. Analytical / Semi-Prep Cartridges HPHN5-CF0310 $265 HPHN5-CF0510 $310 HPHN5-CF1010$385 HAISpeed20-20mm I.D. Prep Guard Cartridges CLIPEUS C18 10µm 20mm I.D. s.s. Prep Guard Cartridges 25x20mm 2/pkg HC181-CF0220 $275 HAICART GUARDS - Stainless Steel Guard Cartridges CLIPEUS C8 5µm 3.2mm I.D. s.s. Guard Cartridges HC108 $160 CLIPEUS C18 5µm CLIPEUS Cyano 5µm CLIPEUS Phenyl 5µm 3.2mm I.D. s.s. Guard Cartridges HC118 $ mm I.D. s.s. Guard Cartridges HC1CN $ mm I.D. s.s. Guard Cartridges HC1PH $170 HAIPEEK GUARDS - PEEK Guard Cartridges CLIPEUS C8 5µm 2.1mm I.D. PEEK Guard Cartridges HC085-CK0221 $165 CLIPEUS C18 5µm 2.1mm I.D. PEEK Guard Cartridges HC185-CK0221 $165 CLIPEUS Cyano 5µm 2.1mm I.D. PEEK Guard Cartridges HCNP5 -CK0221$175 CLIPEUS Phenyl 5µm 2.1mm I.D. PEEK Guard Cartridges HPHN5-CK0221 $175 Methylxanthines 1. Theobromine 2. Theophylline 3. Caffeine 4. Phenol CLIPEUS C18 150x4.6mm P/N CS1546-HC185 25% MeOH/water (unbuffered) 1mL/min, 30ºC, 254nm
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