Eppendorf Consumables it s Your sample. Peak of Perfection. Eppendorf Liquid Handling Consumables

Transcription

1 Eppendorf Consumables it s Your sample Peak of Perfection Eppendorf Liquid Handling Consumables

2 Never Change A Winning Team! Since 50 years consumables perfection for your liquid handling instruments Mastertip for the Eppendorf Biomaster First Combitips for new dispenser Multipette Devices for easy storage and attachment of pipette tips Varitip P and Maxitip for Eppendorf Varipette 4710 Worldwide innovation: Eurotips (US: Envirotips) plus Eurobox (Envirobox) with sliding cover New Comfortips packed in trays for significantly easier handling and perfect fit Start of manufacturing plastic consumables by Eppendorf production

3 New Generation: Patented Combitips plus, providing volume size recognition for Multipette / Repeater plus ept.i.p.s. Motion pipette tips for epmotion, also with filter Best performing ept.i.p.s. LoRetention pipette tips Combitips advanced ept.i.p.s. pipette tips with new storage system Worldwide innovation: ep Dualfilter T.I.P.S. the double layer filter tips ept.i.p.s. Motion pipette tips range with sterile tips

4 Our Investment in Quality and Purity... Absolute Certainty! 50 years experience in manufacturing Liquid Handling laboratory consumables are the basis for the outstanding quality, purity and performance of our products. Eppendorf consumables for the highest quality and purity Manufactured from the carefully selected and purest raw materials which comply with international purity criteria (FDA 21 CFR Olefin Polymers, FDA 21 CFR Antioxidants and Stabilizers for Polymers ) pure to the maximum Fully automatic production in clean room conditions according to VDI 2083 class 6 and to U.S. Fed. Stand. 209D class continuous bioburden and particle monitoring of the plant for impeccable production environment SOP present for storage and every production step risk of human contamination eliminated Frequent checks of production tools guarantee of consistent quality Regular in process quality and functional checks for absolute reproducibility Full traceability for each product implemented full control, each product can be traced back to the material lot Continuous quality assurance throughout the entire production process from the initial material to the finished product. With the strictest control criteria, internally and externally monitored, we guarantee the consistently high quality of our products from lot to lot. In addition to our strict internal process controls, Eppendorf Biopur, PCR clean and Sterile as well as PCR clean purity grades are tested by an independent, external lab lot by lot. This allows us to ensure consistently meet of our customer s high demands when it comes to purity. All certificates may be downloaded on Purity table: see page 19 4

5 Page 1 of 2... Is Your Benefit Certificates - Your safety first! The number of standards, guidelines and regulations that apply to biological, diagnostic and industrial laboratories are constantly increasing and thus the need to provide proof of compliance with these requirements. There are three categories of certificates for Liquid Handling consumables: Lot-specific purity certificates, documenting lot-specific testing by an independent lab for products with purity grades Eppendorf Biopur PCR clean PCR clean and Sterile By entering the respective lot number, lot-specific certificates can be downloaded. General quality certificates as e.g. General Certificates of Purity for PCR clean products Product-specific certificates For values of trace metal For absence of surface active additives With special focus, e.g. filter efficiency for epdualfilter T.I.P.S. Certificates verifying Quality Management/ ISO conformity All certificates may be downloaded on EG-Konformitätserklärung EC Conformity Declaration Das bezeichnete Produkt entspricht den einschlägigen grundlegenden Anforderungen der aufgeführten EG-Richtlinien und Normen. Bei einer nicht mit uns abgestimmten Änderung des Produktes oder einer nicht bestimmungsgemäßen Anwendung verliert diese Erklärung ihre Gültigkeit. The product named below fulfills the relevant fundamental requirements of the EC directives and standards listed. In the case of unauthorized modifications to the product or an unintended use this declaration becomes invalid. Produktbezeichnung, Product name: ept.i.p.s. als Singles, Racks, Reloads, Box, Set as Singles, Racks, Reloads, Box, Set Produkttyp, Product type: Pipetten Spitzen / pipette tips Certificate of Quality ept.i.p.s. ept.i.p.s. pipette tips - Typical values for trace metal Methods). The values in the table indicate typical values of trace metal concentrations which are obtained after incubating ept.i.p.s. pipette tips with conc. nitric acid for 1 hour. (see: Materials and As the indicated values were determined in a one-time measurement, they cannot be guaranteed for every lot of ept.i.p.s. pipette tips. Rather, they give the customer an idea to what extent trace elements can be eluted from ept.i.p.s. pipette tips. Einschlägige EG-Richtlinien/Normen, Relevant EC directives/standards: 98/79/EG, EN 14971, EN 980, EN 556-1*, * EN 8655 (In Kombination mit Eppendorf Pipetten / in combination with Eppendorf pipettes) Vorstand, Board of Management: Projektmanagement, Project Management: Hamburg, Date: * als steril gekennzeichnete Produkte / Products marked as sterile Eppendorf AG Barkhausenweg Hamburg Germany

6 Premium Quality Is Our Standard Perfect fit! Each of your valuable samples deserves the best treatment. See for yourself how Eppendorf pipette tips will save time and reduce costs. With respect to material, fit, design and operating forces our pipette tips set new standards. The close environment of each sample should be adapted to its specific quality and purity needs. This can involve a specific purity level or the absence of certain substances, but also stability, reliability or geometry. The ept.i.p.s. pipette tips from Eppendorf are designed to cover all of the specific needs of your samples. Our ept.i.p.s. Eppendorf Totally Integrated Pipetting System have been developed to work in perfect combination with our pipettes. The results are reduced-force tip attachment and ejection with complete sealing. Additionally, the universal nose cone design allows the tips to be used with pipettes from other manufacturers. In the Eppendorf owned production facilities in the north of Germany, we can maintain the highest standards in the selection and processing of plastic materials. Ideal wetting properties, high transparency and special certified purity levels are the visible expression of this production philosophy. Every tip is manufactured to match specifically to its respective Eppendorf pipettes, thus ensuring the maximum precision and reliability you have come to expect and to rely on. How can you recognize original eppendorf pipette tips? The unmistakable feature of our tips is the ring of drop-shaped relief elements at the edge of the tip crown. Additionally, every tip has the Eppendorf name embossed on the edge. * * for ept.i.p.s. pipette tips excluding ept.i.p.s. standard, ep Dualfilter T.I.P.S. and ept.i.p.s. LoRetention. Further information on 6

7 ept.i.p.s. Standard standard Original, high-quality Eppendorf pipette tips packaged in resealable bags Available in sizes from 10 μl to 10 ml 200 μl, 300 μl and 1,000 μl tips are also available colorcoded yellow and blue ept.i.p.s. Box/epT.I.P.S. Set box Contamination-free transfer of trays to the working box System optimized for use with multichannel pipettes Color-coded trays for simple identification of tips and matching Eppendorf pipette Tips can be attached to the pipette from the refill trays Refill trays and ept.i.p.s. boxes are entirely autoclavable for future use ept.i.p.s. Reloads reloads All components are 100 % recyclable Reduced waste as compared with disposable racks The refill system, depending on tip size, is packaged as either dual-sided or in stack form Reloads are available in two purity levels: Eppendorf Quality and PCR clean ept.i.p.s. Racks racks Eppendorf Biopur pipette tips provide maximum biological purity. Guaranteed PCR clean, sterile, pyrogen-free, ATP-free, bacterial DNA-free, they meet the most stringent requirements of the medical, pharmaceutical and foodstuffs industry as well as those of molecular biology and cell technology Continuous control of each batch by an independent laboratory Batch-related certificates available on request, or go to Packaged in racks of 96, 48 or 24 tips ept.i.p.s. Singles singles Individually wrapped pipette tips in Eppendorf Biopur: guaranteed sterile, RNase-, DNA-, ATP- and pyrogen-free Batch number and expiration date printed on each blisterpack Continuous quality control of each batch by an independent laboratory Batch-specific purity certificates available upon request, or go to Ordering information: see page

8 Aerosols Don t Stand a Chance! ep Dualfilter T.I.P.S. Two filter layers are better than one. Eppendorf ep Dualfilter T.I.P.S. are the first filter tips with a two-phase filter for contamination protection. The unmistakable blue and white filter layers are made of flexible, hydrophobic material to fit perfectly in the tip cone and retain practically 100 % of all aerosols* and bio molecules (see p. 9). This unique filtering effect is achieved using various, well-defined pore sizes in the two filter layers. The white layer that faces the sample retains drops, splashes and aerosols (see p. 3). The blue layer above it forms a highly-effective double barrier that reliably binds biomolecules. The air flow rate through the filter is the same as with single-layer filters it simply makes your rapid pipetting tasks much safer. The ep Dualfilter T.I.P.S. hydrophobic filter surface also enables easier and complete recovery of samples. Product features und benefits Two filter layers provide dual protection Dual protection for pipette and sample Dual protection from aerosols and biomolecules Free of PCR inhibitor additives Sterile, Eppendorf PCR clean and pyrogen-free Batch certified * Aerosol: Aerosol particle and the air or dispersion of solid or liquid particles surrounding it in a gas, usually air. Pipette Dual protection against contamination right from the start. ep Dualfilter T.I.P.S. are manufactured to the highest possible quality from pure, non-recycled materials under cleanroom conditions. They are characterized by defined flow dynamics, low wettability and high thermal stability. Pipette cone Pipette tip Filter ep Dualfilter T.I.P.S. are sterilized by electron beams and certified pyrogen-free and PCR clean (free from human DNA, DNase, RNase and PCRinhibiting substances). Individual batch certification can be downloaded from Without a filter, the pipette is exposed to contamination by samples and aerosols. Conventional single-layer filters do not fully block particles and molecules. Only ep Dualfilter T.I.P.S. provide reliable protection even against the finest impurities. 8

9 Scientifically Proven No filter protects better than ep Dualfilter T.I.P.S. Filter tips from different manufacturers were tested in a blind trial at the Fraunhofer-Institut für Toxikologie und Experimentelle Medizin (Fraunhofer Institute for Toxicology and Experimental Medicine ITEM) in Hannover. Testing focused on the prevention of pipette cone contamination by aerosols. Filter effect with regard to salt aerosols and biomolecules (DNA fragments) was examined. Quantitative determination of DNA fragments was performed using real-time PCR. With regard to particle permeability, ep Dualfilter T.I.P.S. filter tips are 55 to 677 times more effective than the filter tips tested from other manufacturers. ep Dualfilter T.I.P.S. filter tips were 21 to 600 times more effective when protection against DNA fragments was examined for the first time. The results indicate that ep Dualfilter T.I.P.S. filter tips are much better at protecting against aerosols than other commercially available filter tips. Aerosol/particle penetration of various filter tips Particle permeability DNA permeability ep Dualfilter T.I.P.S. Filter tips Manufacturer A Filter tips Manufacturer B Filter tips Manufacturer C Filter tips Manufacturer D Filter tips Manufacturer E 0.13% ±0.11% 0.02% ±0.01% 15.00% ±5.40% 0.43% ±0.03% 88.00% ±7.00% 12.00% ±5.20% 9.70% ±2.60% 0.87% ±0.40% 15.00% ±4.00% 0.96% ±0.11% 7.20% ±1.70% 1.40% ±0.39% Ordering information: see page 24 9

10 Best Possible Results ept.i.p.s. LoRetention Increased sensitivity with maximum reproducibility Do you depend on your precious samples? Then don t leave any valuable residues in your tips when pipetting solutions that contain detergents! Based on a unique and innovative method of material modification, the new Eppendorf ept.i.p.s. LoRetention pipette tips allow you to transfer almost 100 % of the liquid picked up ( pearl effect ) for maximum recovery with unbeaten precision. The pipetting of liquids containing detergents is ubiquitous in modern laboratory processes. The lower surface tension involved has a marked influence on the flow and dosing properties of samples, thus making it noticeably more difficult to perform reproducible work with these liquids. The Pearl Effect The ultrahydrophobic, extremely homogenous surface of the ept.i.p.s. LoRetention pipette tips is achieved through an innovative treatment at the molecular level the Pearl Effect Technology. The tips are uncoated, free from additives, and do not bleed into the sample. Liquids roll off completely, so that only a tiny drop remains in the tip. In comparison, the adhesive layer of liquid in standard tips retains considerably more sample material. For users of the new ept.i.p.s. LoRetention pipette tips, this unique material property guarantees maximum sample recovery, improved reproducibility and higher sensitivity. Available in purity grade of PCR clean and Eppendorf Quality, also as ep Dualfilter T.I.P.S. Product features and benefits Ultrahomogeneous surface for maximum reproducibility Minimum sample loss thanks to ultrahydrophobic surface Significantly reduced build up of foam when pipetting Chemical resistant and autoclavable The reusable seal supplied with the reloads can also be used to label existing ept.i.p.s. boxes. Simple differentiation of racks and reloads through a new, clear lid with a white seal. When open, the writing LoRetention can be seen from the inside. 2 1 The Pearl Effect a comparison of the amount of residual liquid when pipetting solutions that contain detergents: Maximum recovery with ept.i.p.s. LoRetention Significant loss of sample using standard tips. 10

11 The Facts Speak for Themselves Maximum reproducibility with ept.i.p.s. LoRetention Reduce costs, increase reproducibility In particular with sensitive PCR and real-time PCR applications, low-retention surfaces can increase reproducibility and notably reduce the loss of expensive reagents depending on the reagent solution, this can be by up to 5 %! Versatile application ept.i.p.s. LoRetention pipette tips are ideally suited for all applications with samples that contain detergents: PCR and real-time PCR preparation of enzyme solutions and master mixes Isolation, purification and denaturation of proteins Restriction digestions and ligations Pipetting DNA ladders during gel electrophoresis Quality and purity à la Eppendorf It goes without saying that the new ept.i.p.s. LoRetention pipette tips have all the valued properties of the proven Eppendorf ept.i.p.s. They are produced in high-precision manufacturing processes, can be autoclaved and are extremely resistant to chemicals. This Eppendorf quality is also available in the PCR clean (free of human DNA, DNase, RNase, and PCR inhibitors) purity grade. For maximum contamination protection, ept.i.p.s. LoRetention pipette tips are also available as ep Dualfilter T.I.P.S. ept.i.p.s. LoRetention perform considerably better in terms of precision and sample recovery than low-retention tips from other manufacturers.* ep T.I.P.S. LoRetention 6,0 Low-Retention Competitor A 5,0 Low-Retention Competitor M Residual liquid [µl] 6,0 5,0 4,0 3,0 2,0 0,1% Triton X-100 PCR-Mastermix Residual liquid [µl] 4,0 3,0 2,0 1,0 0,0 Untreated Acetonitrile Acetone Isopropanol Autoclaved 1,0 0,0 ept.i.p.s. ept.i.p.s LoRetention Comparison of residual liquid from ept.i.p.s. LoRetention with standard ept.i.p.s. pipette tips and low-retention tips of other manufacturers. 200 µl tips were used to pipette a commercial PCR master mix and the detergent 0.1 % Triton X-100. Once the liquid had been dispensed, the residual liquid in the tips was determined. The measurements were repeated several times and the standard deviation determined. ept.i.p.s. LoRetention pipette tips resulted in the lowest residual liquid, as well as the smallest standard deviation. Competitor A Low-Retention Competitor M Low-Retention Competitor S Low-Retention Comparison of resistance of the low-retention property when subjected to solvents or autoclaving. 200 µl ept.i.p.s. LoRetention pipette tips and low-retention tips of other manufacturers were autoclaved or treated with the solvent specified. An enzymatic buffer containing detergents was then pipetted and the residual moisture was determined. ept.i.p.s. LoRetention pipette tips resulted in reproducible low levels of residual liquid with minimum standard deviation. Low-retention tips from other manufacturers are less resistant to chemicals or autoclaving: this resulted in an increase in residual liquid and standard deviation. * Read our Application Notes 218 and 192 at for more information Ordering information: see page 20 and 24 11

12 Long-Distance Tips! Xtra long for best pipetting results! With the ept.i.p.s. L pipette tips you will always receive best results while pipetting from and into conical tubes, high reagent bottles, narrow deep vessels and especially Eppendorf Deepwell Plates. The long and slim design of these elongated pipette tips give you free access to your sample with reduced risk of touching the sides of tubes or wells. Cross-contamination during pipetting can be avoided to a minimum. Even smallest volumes in depwell plates of 96 and 384 wells can be almost completely recovered from the bottom. Product features and benefits Highest protection for pipette and sample with ep Dualfilter T.I.P.S. L Available in the purity standards Eppendorf Quality, PCR clean, PCR clean/sterile and Eppendorf Biopur Safe access to Eppendorf Deepwell Plates or other deep and narrow vessels ept.i.p.s. L are available in volumes of: μl L 46 mm long, μl L 103 mm long and 1 10 ml L 243 mm long Ordering information: see page

13 Loading and Filling! Eppendorf Microloader Eppendorf GELoader These unique tips are ideally suited to fill microcapillaries used for microinjection. The extremely long, fine and flexible tip provides also the ideal solution for all kind of applications in which additional reach is needed while pipetting smallest volumes. Eppendorf s GELoader Tips have been designed to simplify the loading of samples onto polyacrylamide gels. These flexible, long and narrow tips prevent the gels from being damaged whilst allowing optimal handling of smallest volumes. Product features and benefits Rack package can be sterilized Ideal for recovering surplus solution from the capillary Product features and benefits Special tip for gel electrophoresis GELoader Tips and rack autoclavable (121 C, 20 min.) Highest precision and accuracy when used with Eppendorf pipettes for 0.5 to 10 μl (gray control button) Rear filling of Microcapillary using Microloader and Research plus pipette Ordering information: see page 25 13

14 Reliability for Unattended Automation ept.i.p.s. Motion Eppendorf Totally Integrated Pipetting System for Automation Eppendorf has a long history in automated liquid handling. For routine pipetting with the best precision in the industry it is important to have a perfect system of tips and automate. The ept.i.p.s. Motion have been developed to work in perfect combination with our epmotion platform. Product features and benefits More safety Each tip is checked for straightness before packaging Color-coded trays for direct volume identification Double O-ring on dispensing tools assures an optimal fit each time Optical sensor automatically identified tip type Flexible handling Three volume ranges available with and w/o filter Available in multiple purity grades Easily exchangeable, no additional labware files required Also available as reload system Eco-friendly Reload System Easy conversion with TipHolder Adapter TipHolder Adapter is autoclavable Reduces waste by up to 40 % The ept.i.p.s. Motion racks consist of two options for simple integration into the epmotion liquid handling workstation: A single use box with color coded trays for easy volume identification and a TipHolder Adapter to use the trays as a Reload-System. With color coded trays you can quickly and easily see the volume range of the tip and assure an even higher level of sample safety. The new Reload trays also offer a more environmentally friendly option and will be delivered in a PET blister packaging with a sealed cap assuring the same well established quality as all Eppendorf tips. The optional TipHolder Adapter (autoclavable) replaces the normal tip box component and reduces waste by up to 40 %. In the Eppendorf owned production facilities in the north of Germany, we can maintain the highest standards a perfect match for the most demanding user. Ordering information: see page 27 14

15 Positive Displacement Tips for Eppendorf Biomaster Eppendorf Mastertips Enjoy contamination-free pipetting with the Positive Displacement Pipette, an adjustable pipette for the volume range from 1 μl to 20 μl. With the Positive Displacement Tip, the Eppendorf Mastertip, the pipette functions according to the positive-displacement principle; thus, the formation of aerosols a common cause of contamination is eliminated. Product features and benefits Ready-for-use piston integrated in tip for contamination-free pipetting in positive displacement mode Ideal for liquids that are viscous (e.g. glycerol) or have high vapour pressure (e.g. ethano, acetone) 96 positive displacement tips per rack Eppendorf Biomaster Eppendorf Varitips* for Eppendorf Varipette * The pipette tips for the Varipette are tailored to different vessels. For example, the Varitip P is designed for aspirating 1 ml to 10 ml from beakers, and it pipettes according to the positive displacement principle. The Varitip S for 2.5 ml to 10 ml forms a system with the Varipette, and it is used for aspirating liquids from tall, narrow-neck vessels. Product features and benefits Eppendorf Varipette * Ordering information: see page Varitip P for (fig. 1) for aspirating 1 ml to 10mL from beakers using a positive displacement technique Varitip S (fig. 2): The dosing part, in combination with the Maxitip (fig. 3) forms a system for aspiration of between 2.5 ml and 10 ml liquid from high, narrow-mouth vessels air displacement principle No carryover as Maxitips are exchanged Valve for Maxitip ensures drip-free dispensing of liquids with a high vapour pressure Maxitip is graduated for dispensing accurate volumes of nonaqueous liquids * US product names: Maxipettor, Maxitip P, Maxitip S 15

16 Time for a new Original! Eppendorf Combitips advanced The invention of the Eppendorf hand dispenser Multipette /Repeater and the Eppendorf Combitips marks a milestone in the field of Liquid Handling! Executing long pipetting respectively dispensing series is remarkably simpler and faster. With the innovative sensor technology for automatic Combitip recognition, this dispensing system has become an indispensable tool for every laboratory. The increasingly high demands of modern laboratory work have also heightened the requirements for a highprecision dispensing tip. Our experts have thoroughly optimized the Combtips using sophisticated engineering. The result: A revolutionary 360 evolution! Setting a completely new standard in dispensing tips. The Combitips principle Positive displacement principle (comparable to a syringe) High-precision-dispensing regardless of the density and viscosity of the liquid Prevents aerosol contamination with hermetically sealed piston for secure dispensing and provides protection from radioactive and toxic substances Quick dispensing of long series with precise, repeated dispensing of identical volumes (in combination with the Multipette/Repeater) The Third Dimension of Tip Technology Unmistakably Eppendorf Also the Combitips advanced feature the characteristic Eppendorf 3D design on the upper rim of the Combitip, allowing easily and confidently recognizing the Eppendorf original! Ergonomic Design Unique funnel geometry prevents damage to gloves and ensures comfortable handling Individually color coded Quick identification of the desired Combitips facilitates the workflow Elongated tips The complete emptying of all common tubes prevents sample loss Variety and selection With 9 volume sizes (0.1 ml to 50 ml) and several purity levels you will always find the perfect Combitip for your application. The tips in Eppendorf Biopur are individually blister-wrapped and feature an access tab which makes them easier to open, even with gloves Intelligent packaging With the redesigned dispenser box with extricable chute, Combitips advanced are always within reach. Portioning in 4 bags (25 pcs. each) ensures dust-free storage. Always easy to reach The new Combitips Rack features trouble-free and single-handed operation during dispensing work. The rack holds up to 8 Combitips advanced in sizes ranging from 0.1 ml to 10 ml. The transparent tray allows clearly Combitips size identification using color coding. Ordering information: see page 26 16

17 Eppendorf Combitips advanced for Multipette plus, Multipette stream and Multipette Xstream Technical specifications Combitips advanced Min./max. volume Increment/ step size for Multipette plus and Combitips advanced system Max. dispensing volume Volume Inaccuracy* 1 Imprecision* ml 1 µl 1 µl 20 µl 2 µl ±1.6% ±3.0% 20 µl 20 µl ±1.0% ±2.0% 0.2 ml 2 µl 2 µl 40 µl 4 µl ±1.3% ±2.0% 40 µl 40 µl ±0.8% ±1.5% 0.5 ml 5 µl 5 µl 100 µl 10 µl ±0.9% ±1.5% 100 µl 100 µl ±0.8% ±0.6% 1 ml 10 µl 10 µl 200 µl 20 µl ±0.9% ±0.9% 200 µl 200 µl ±0.6% ±0.4% 2.5 ml 25 µl 25 µl 500 µl 50 µl ±0.8% ±0.8% 500 µl 500 µl ±0.5% ±0.3% 5 ml 50 µl 50 µl 1,000 µl 100 µl ±0.6% ±0.6% 1,000 µl 1,000 µl ±0.5% ±0.25% 10 ml 100 µl 100 µl 2,000 µl 200 µl ±0.5% ±0.6% 2,000 µl 2,000 µl ±0.5% ±0.25% 25 ml 250 µl 250 µl 5,000 µl 500 µl ±0.4% ±0.6% 5,000 µl 5,000 µl ±0.3% ±0.25% 50 ml 500 µl 500 µl 10,000 µl 1,000 µl ±0.3% ±0.5% 10,000 µl 10,000 µl ±0.3% ±0.3% for Multipette stream / Multipette Xstream and Combitips advanced system 0.1 ml 1 µl 0.1 µl 0.1 ml 10 µl ±1.6% ±2.5% 50 µl ±1.0% ±1.5% 100 µl 100 µl ±1.0% ±0.5% 0.2 ml 2 µl 0.2 µl 0.2 ml 20 µl ±1.3% ±1.5% 100 µl ±1.0% ±1.0% 200 µl 200 µl ±1.0% ±0.5% 0.5 ml 5 µl 0.5 µl 0.5 ml 50 µl ±0.9% ±0.8% 250 µl ±0.9% ±0.5% 500 µl 500 µl ±0.9% ±0.3% 1 ml 10 µl 1 µl 1 ml 100 µl ±0.9% ±0.55% 500 µl ±0.6% ±0.3% 1 ml 1,000 µl ±0.6% ±0.2% 2.5 ml 25 µl 2.5 µl 2.5 ml 250 µl ±0.8% ±0.45% 1,250 µl ±0.5% ±0.3% 2.5 ml 2,500 µl ±0.5% ±0.15% 5 ml 50 µl 5 µl 5 ml 500 µl ±0.8% ±0.35% 2,500 µl ±0.5% ±0.25% 5 ml 5,000 µl ±0.5% ±0.15% 10 ml 100 µl 10 µl 10 ml 1,000 µl ±0.5% ±0.25% 5,000 µl ±0.4% ±0.25% 10 ml 10,000 µl ±0.4% ±0.15% 25 ml 250 µl 25 µl 25 ml 2,500 µl ±0.3% ±0.35% 12,500 µl ±0.3% ±0.25% 25 ml 25,000 µl ±0.3% ±0.15% 50 ml 500 µl 50 µl 50 ml 5,000 µl ±0.3% ±0.5% 25,000 µl ±0.3% ±0.2% 50 ml 50,000 µl ±0.3% ±0.15% * 1 The data for imprecision (random error) and inaccuracy (systematic error) according to EN ISO 8655 only apply when using original Eppendorf Combitips advanced. 17

18 Volume table: Combitips advanced /Multipette plus Volume Table for Using Combitips advanced with Multipette plus Position of the volume selection dial: 0.5 1/ / / / / Maximum number of dispensing volumes: / / / / / Combitips advanced with the following volumes: 0.1 ml 1 μl 2 μl 3 μl 4 μl 5 μl 6 μl 7 μl 8 μl 9 μl 10 μl 11 μl 12 μl 13 μl 14 μl 15 μl 16 μl 17 μl 18 μl 19 μl 20 μl 0.2 ml 2 μl 4 μl 6 μl 8 μl 10 μl 12 μl 14 μl 16 μl 18 μl 20 μl 22 μl 24 μl 26 μl 28 μl 30 μl 32 μl 34 μl 36 μl 38 μl 40 μl 0.5 ml 5 μl 10 μl 15 μl 20 μl 25 μl 30 μl 35 μl 40 μl 45 μl 50 μl 55 μl 60 μl 65 μl 70 μl 75 μl 80 μl 85 μl 90 μl 95 μl 100 μl 1.0 ml 10 μl 20 μl 30 μl 40 μl 50 μl 60 μl 70 μl 80 μl 90 μl 100 μl 110 μl 120 μl 130 μl 140 μl 150 μl 160 μl 170 μl 180 μl 190 μl 200 μl 2.5 ml 25 μl 50 μl 75 μl 100 μl 125 μl 150 μl 175 μl 200 μl 225 μl 250 μl 275 μl 300 μl 325 μl 350 μl 375 μl 400 μl 425 μl 450 μl 475 μl 500 μl 5 ml 50 μl 100 μl 150 μl 200 μl 250 μl 300 μl 350 μl 400 μl 450 μl 500 μl 550 μl 600 μl 650 μl 700 μl 750 μl 800 μl 850 μl 900 μl 950 μl 1.0 ml 10 ml 100 μl 200 μl 300 μl 400 μl 500 μl 600 μl 700 μl 800 μl 900 μl 1.0 ml 1.1 ml 1.2 ml 1.3 ml 1.4 ml 1.5 ml 1.6 ml 1.7 ml 1.8 ml 1.9 ml 2.0 ml 25 ml* 250 μl 500 μl 750 μl 1.0 ml 1.25 ml 1.5 ml 1.75 ml 2.0 ml 2.25 ml 2.5 ml 2.75 ml 3.0 ml 3.25 ml 3.5 ml 3.75 ml 4.0 ml 4.25 ml 4.5 ml 4.75 ml 5.0 ml 50 ml* 500 μl 1.0 ml 1.5 ml 2.0 ml 2.5 ml 3.0 ml 3.5 ml 4.0 ml 4.5 ml 5.0 ml 5.5 ml 6.0 ml 6.5 ml 7.0 ml 7.5 ml 8.0 ml 8.5 ml 9.0 ml 9.5 ml 10 ml * 25 ml and 50 ml Combitips plus must be used with the supplied adapter. Ordering information: see page 26 18

19 Eppendorf Purity Levels for Liquid Handling Consumables For more information go to and on page 4 Continuous quality control for the following relevant criteria: Function, tightness, precision Low wetting High chemical resistance High thermal resistance High resistance to centrifugation forces* 1 High transparency Precisely shaped Lot testing* 2 (certified) for the following purity criteria: Human DNA-free DNA-free (Human- + bacteria DNA) DNase-free NEW! RNase-free PCR-inhibitor-free NEW! ATP-free Pyrogen-free (endotoxin-free) NEW! Sterile (Ph.Eur./USP) Eppendorf products Tips ept.i.p.s. Standard + Box + Set ept.i.p.s. Reloads ept.i.p.s. Racks ept.i.p.s. Singles * 3 ep Dualfilter T.I.P.S. Combitips advanced * 6 * 7 * 3 ept.i.p.s. LoRetention ept.i.p.s. Motion ept.i.p.s. Motion filtertips Tubes Eppendorf Safe-Lock Tubes * X DNA + Protein LoBind Tubes PCR Tubes (PCR + real-time PCR) Cap Strips (PCR + real-time PCR) Plates and accessories Deepwell Plates + Microplates Protein LoBind Deepwell Plates DNA LoBind Deepwell Plates twin.tec PCR Plates (PCR and real-time PCR) twin.tec microbiology PCR Plates Heat Sealing Film and Foil PCR, Storage Films + Foils Plate Lid UVette * 4 * 1 For accurate details regarding resistance to centrifugation, please refer to the respective product pages. * 2 Batch-specific certificate can be downloaded from * 3 Individually packaged. * 4 Additionally sterile. * 5 Additonally free of protein. * 6 Only available in North America * 7 Not available in North America Technical specifications subject to change. 19

20 TIPS Volume range/ept.i.p.s. ept.i.p.s. LoRetention (all tips shown are actual size) Standard Reloads Reloads Eppendorf Quality Eppendorf Quality PCR clean µl, 34 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips* 1 10 trays of 96 tips = 960 tips* LoRetention LoRetention µl, 40 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips 10 trays of 96 tips = 960 tips µl L, 46 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips 10 trays of 96 tips = 960 tips LoRetention LoRetention µl, 53 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips* 1 10 trays of 96 tips = 960 tips* yellow LoRetention LoRetention µl, 55 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips 10 trays of 96 tips = 960 tips yellow ,000 µl, 71 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips 10 trays of 96 tips = 960 tips blue LoRetention LoRetention 20

21 Box Set Singles Racks Eppendorf Quality Eppendorf Quality Eppendorf Biopur (sterile) Eppendorf Biopur (sterile) 1 reusable box incl. 96 tips 1 reusable box incl. 5 trays of 96 tips LoRetention 1 reusable box incl. 96 tips 1 reusable box incl. 5 trays of 96 tips 100 tips, individually wrapped 5 racks of 96 tips = 480 tips reusable box incl. 96 tips 1 reusable box incl. 5 trays of 96 tips LoRetention 1 reusable box incl. 96 tips 1 reusable box incl. 5 trays of 96 tips LoRetention 100 tips, individually wrapped 5 racks of 96 tips = 480 tips reusable box incl. 96 tips 1 reusable box incl. 5 trays of 96 tips racks of 96 tips = 480 tips 1 reusable box incl. 96 tips 1 reusable box incl. 5 trays of 96 tips LoRetention 100 tips, individually wrapped 5 racks of 96 tips = 480 tips

22 TIPS Volume range/pipette tip (all tips shown are actual size) Standard Reloads Reloads Eppendorf Quality Eppendorf Quality PCR clean 50 1,250 µl, 76 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips 10 trays of 96 tips = 960 tips ,250 µl L, 103 mm 2 bags of 500 tips = 1,000 tips 10 trays of 96 tips = 960 tips 10 trays of 96 tips = 960 tips ,500 µl, 115 mm 5 bags of 100 tips = 500 tips 10 trays of 48 tips = 480 tips 10 trays of 48 tips = 480 tips ,000 µl, 120 mm 5 bags of 100 tips = 500 tips ml, 165 mm 2 bags of 100 tips = 200 tips ml L, 243 mm (Image reduced in size) 2 bags of 100 tips = 200 tips Fits in deep and narrow test tubes, cell culture bottles and measuring pistons

23 Box Set Singles Racks Eppendorf Quality Eppendorf Quality Eppendorf Biopur (sterile) Eppendorf Biopur (sterile) 1 reusable box incl. 96 tips 1 reusable box incl. 5 trays of 96 tips racks of 96 tips = 480 tips 1 reusable box incl. 96 tips 5 racks of 96 tips = 480 tips reusable box incl. 48 tips 1 reusable box incl. 5 trays of 48 tips racks of 48 tips = 240 tips 1 reusable box incl. 24 tips racks of 24 tips = 120 tips

24 Volume range/ep Dualfilter T.I.P.S. ep Dualfilter T.I.P.S. LoRetention (all tips shown are actual size) Racks PCR clean, sterile incl. pyrogen-free µl S, 34 mm 10 racks of 96 tips = 960 tips LoRetention µl M, 40 mm 10 racks of 96 tips = 960 tips µl L, 46 mm 10 racks of 96 tips = 960 tips LoRetention 2 20 µl, 53 mm 10 racks of 96 tips = 960 tips µl, 53 mm 10 racks of 96 tips = 960 tips LoRetention µl, 55 mm 10 racks of 96 tips = 960 tips µl, 55 mm 10 racks of 96 tips = 960 tips LoRetention 50 1,000 µl, 76 mm 10 racks of 96 tips = 960 tips LoRetention 24

25 Volume range/ep Dualfilter T.I.P.S. (most tips shown are actual size) Racks PCR clean, sterile incl. pyrogen-free 50 1,250 µl L, 103 mm 5 Racks of 96 Tips = 480 Tips ,000 µl, 120 mm 5 racks of 24 tips = 120 tips ml L, 243 mm 100 tips, individually wrapped (Image reduced in size) Special tips TIPS Volume range/pipette tip GELoader μl, 62 mm 2 racks of 96 tips = 192 tips Microloader μl, 100 mm 2 racks of 96 tips = 192 tips Mastertip 20 μl, 52 mm 5 racks of 96 tips = 480 tips (Includes ready-for-use piston)

26 Combitips advanced and accessories Ordering information Combitips advanced Color coding Eppendorf Quality PCR clean Eppendorf Biopur 0.1 ml* White ml* Light blue ml* Purple ml* Yellow ml* Green ml* Blue ml* Orange ml* Red ml* Light gray * set of 100 Accessories Color coding Eppendorf Quality Eppendorf Biopur 25 ml adapter (1 pc.) Red ml adapter (1 pc.) Light gray ml adapter (7 pc.) Red ml adapter (7 pc.) Light gray Combitip Rack (for 8 Combitips advanced, 0.1 ml 10 ml) Varitip P and Varitip S for Varipette Ordering information Description Varitip P Starter Kit (100 Maxitips, 10 dispensing parts, 10 valves) Order no. for aspirating from smaller vessels Varitips P (set of 100) Varitip S consisting of: Barrels with pistons (for Varitip S, set of 30) for aspirating from Narrow-neck vessels and volumetric flasks Maxitip (for Varitip S, set of 200) Valves (for Varitip S, set of 100)

27 TIPS epmotion automated pipetting system Ordering information Description Order no. ept.i.p.s. Motion pipette tips Automatic pipette tips in individual racks for use on the epmotion. The tip type and size is automatically recognized on the device. 96 ept.i.p.s./rack, 10 racks per set. The refill racks from the reload products can be placed in an autoclavable tip holder adapter. Three purity levels: Eppendorf Quality, PCR clean and sterile. Pipette tips available with or without a filter. Testing of production batches (certified). Without filter 50 μl, sterile, free of pyrogens, volume range 1 50 μl, tips in racks μl, sterile, free of pyrogens, volume range μl, tips in racks ,000 μl, sterile, free of pyrogens, volume range 40 1,000 μl, tips in racks μl, Eppendorf Quality, volume range 1 50 μl, tips in racks μl, Eppendorf Quality, volume range μl, tips in racks ,000 μl, Eppendorf Quality, volume range 40 1,000 μl, tips in racks μl, Eppendorf Quality, Reloads, volume range 1-50 μl, tips μl, Eppendorf Quality, Reloads, volume range μl, tips ,000 μl, Eppendorf Quality, Reloads, volume range 40-1,000 μl, tips With filter 50 μl, PCR clean, sterile, free of pyrogens, volume range 1 50 μl, tips in racks μl, PCR clean, sterile, free of pyrogens, volume range μl, tips in racks ,000 μl, PCR clean, sterile, free of pyrogens, volume range 40 1,000 μl, tips in racks μl, PCR clean, volume range 1-50 μl, tips in Racks μl, PCR clean, volume range μl, tips in Racks μl, PCR clean, volume range 40-1,000 μl, tips in Racks μl, PCR clean, Reloads, volume range 1 50 μl, tips μl, PCR clean, Reloads, volume range μl, tips ,000 μl, PCR clean, Reloads, volume range 40 1,000 μl, tips Reservoir rack modules Are inserted in a Reservoir rack. They can be temperature controlled with a thermal module for heating and cooling. PCR 0.2 ml, for ml PCR tubes PCR 0.5 ml, for ml PCR tubes Eppendorf Safe-Lock, for 4 0.5/1.5/2 ml tubes Tubes Ø12 mm, for 4 Ø 12 mm tubes Tubes Ø16 mm, for 4 Ø 16 mm tubes ml conical tubes, for 4 Ø 17 mm tubes ml conical tubes, for 2 Ø 29 mm tubes Reservoir adapter 30 ml, for 1 epmotion reservoir 30 ml Reservoir adapter 100 ml, for 1 epmotion reservoir 100 ml ml reservoir, also for use in the epmotion VAC, 10 pcs./set, made of PP Falcon is a registered trademark of Becton Dickinson. 27

28 Eppendorf pipette/ept.i.p.s. combinations ept.i.p.s. (μl) L (L = Long) ept.i.p.s. LoRetention (μl) * L Volume marking at [μl or ml] 2.5; ; 50; ; 100; Pipette dark gray medium gray light gray yellow orange Eppendorf Research family / Eppendorf Research plus Fixed volume 10 μl, 20 μl gray* 7 10 μl, 20 μl, 25 μl, 50 μl, 200 μl yellow 200 μl, 250 μl, 500 μl, 1,000 μl blue Adjustable volume 0.1 μl 2.5 μl * μl 10 μl 2 μl 20 μl light gray* 7 2 μl 20 μl yellow 10 μl 100 μl 20 μl 200 μl 20 μl 300 μl* μl 1,000 μl 500 μl 5,000 μl 1 ml 10 ml Multi-channel 0.5 μl 10 μl 10 μl 100 μl 30 μl 300 μl Eppendorf Reference Fixed volume 1 μl 10 μl 10 μl 200 μl yellow 200 μl 1,000 μl blue 1500 μl 2,500 μl Adjustable volume 0.1 μl 2.5 μl * μl 10 μl 2 μl 20 μl gray 2 μl 20 μl yellow 10 μl 100 μl 50 μl 200 μl yellow 50 μl 250 μl blue 100 μl 1,000 μl 500 μl 2,500 μl Eppendorf Research pro / Eppendorf Xplorer Single-channel 0.5 μl 10 μl 5 μl 100 μl 15/20 μl 300 μl 50 μl 1,000 μl 0.1/0.25 μl 5 ml 0.5 ml 10 ml* 8 Multi-channel 0.5 μl 10 μl 5 μl 100 μl 15/20 μl 300 μl 50 μl 1,200 μl * 1 = limited volume; * 2 = only for the Research fi 10 μl, 20 μl; * 3 = not suitable for the Reference fi 200 μl and 250 μl; * 4 = the maximum permissible error given in the technical data for the pipette might not be met * 5 = on the 1 10 ml pipette, when the 1 10 ml tip long is used, the quoted tolerance range increases by a factor of 2 compared to when the standard tip is used * 6 = LoRetention ept.i.p.s. are intended for detergent containing solutions. The maximum permissible error given in the technical data for the pipette might not be met when dispensing aqueous solutions. * 7 = only Research plus; * 8 = only Xplorer Technical specifications subject to change

29 / L* 4 / L* 4 / L* 4 / L* 4 Allocation table for ept.i.p.s. 50 1, ,250 ml /50 1,250 ml L (L = Long) 500 2, , ml /1 10 ml L (L = Long) GELoader Microloader 50 1, ; ml: 1; 5; ml L: each ml blue green / dark green red purple turquoise Tip / L* 4 / L* 5 / L* 5 29

30 Eppendorf pipette/ep Dualfilter T.I.P.S. combinations ept.i.p.s. Dualfilter T.I.P.S. (μl) S (S = Small) M (M = Medium) L (L = Long) ep Dualfilter T.I.P.S. LoRetention (µl) S* Volume marking at [μl or ml] 2.5; ; 50; 100 Pipette dark gray medium gray light gray yellow yellow Eppendorf Research family / Eppendorf Research plus Fixed volume 10 μl gray* 7 20 μl gray* 7 10 μl, 20 μl, 25 μl, 50 μl, 200 μl yellow 200 μl, 250 μl, 500 μl, 1,000 μl blue Adjustable volume 0.1 μl 2.5 μl * μl 10 μl 2 μl 20 μl light gray* 7 * 2 2 μl 20 μl yellow 10 μl 100 μl 20 μl 200 μl 20 μl 300 μl* μl 1,000 μl 500 μl 5,000 μl 1 ml 10 ml Multi-channel 0.5 μl 10 μl 10 μl 100 μl 30 μl 300 μl Eppendorf Reference Fixed volume 1 μl 10 μl 10 μl 200 μl yellow 200 μl 1,000 μl blue 1500 μl 2,500 μl Adjustable volume 0.1 μl 2.5 μl / L* μl 10 μl 2 μl 20 μl gray 2 μl 20 μl yellow 10 μl 100 μl 50 μl 200 μl yellow 50 μl 250 μl blue 100 μl 1,000 μl 500 μl 2,500 μl Eppendorf Research pro / Eppendorf Xplorer Single-channel 0.5 μl 10 μl 5 μl 100 μl 15/20 μl 300 μl 50 μl 1,000 μl 0.1/0.25 μl 5 ml 0.5 ml 10 ml* 8 Multi-channel 0.5 μl 10 μl 5 μl 100 μl 15/20 μl 300 μl 50 μl 1,200 μl * 1 = limited volume; * 2 = only for the Research fi 10 μl, 20 μl; * 3 = not suitable for the Reference fi 200 μl and 250 μl; * 4 = the maximum permissible error given in the technical data for the pipette might not be met * 5 = on the 1 10 ml pipette, when the 1 10 ml tip long is used, the quoted tolerance range increases by a factor of 2 compared to when the standard tip is used * 6 = LoRetention ept.i.p.s. are intended for detergent containing solutions. The maximum permissible error given in the technical data for the pipette might not be met when dispensing aqueous solutions. * 7 = only Research plus; * 8 = only Xplorer Technical specifications subject to change 30

31 / L* 4 / L* 4 Allocation table for ep Dualfilter T.I.P.S , ,250 L (L = Long) 100 5, ml L (L = Long) ; 100; ; 100; ml L each ml yellow orange blue dark green purple individually wrapped / L* 4 / L* 5 * 3 / L* 4 * 4 / L* 4 / L* 5 * 1 31

32 Make Your Lab a Better Place The Eppendorf Liquid Handling Instruments Portfolio As the inventor of the microliter system, we at Eppendorf have over fifty years experience in precise manual and automatic dispensing and transferring of the smallest quantities of liquids. The standards defined by Eppendorf quality assurance (for example, precision and accuracy guidelines) clearly surpass the standards required to obtain these results, and guarantee the reproducibility that our customers have come to depend on. Our PhysioCare Concept incorporates growing ergonomic demands into all that we do. The use of our liquid handling products has been proven to reduce physical strain at the workstation to a minimum. This has been perfected by the ergonomic design of our products and flawless fit of device to consumable (e.g., measurably reduced ejecting force when pipetting). For more information visit Your local distributor: Eppendorf AG Hamburg Germany Tel: Fax: eppendorf@eppendorf.com Eppendorf North America, Inc. 102 Motor Parkway Hauppauge, N.Y USA Tel: Toll free phone: Fax: info@eppendorf.com Application Support Europe: Tel: (9 ct/min aus dem dt. Festnetz; Mobilfunkhöchstpreis 42 ct/min) support@eppendorf.com North America: Tel: techserv@eppendorf.com Asia Pacific: Tel: support_asiapacific@eppendorf.com ENVIROTIPS is a trademark of Eppendorf North America, Inc., USA. eppendorf, ept.i.p.s., Combitips advanced, Eppendorf Biomaster, Eppendorf Research, PhysioCare Concept, Eppendorf Xplorer, Eppendorf Referece, Repeater, Eppendorf Varipette, Eppendorf LoBind, epmotion T.I.P.S., Eppendorf Biopur, epdualfilter T.I.P.S., Mastertip, Eurotips, Eppendorf Biomaster, Eppendorf Varipette, Multipette, Multipette stream, Multipette Xstream, Combitips plus, twin.tec, UVette and epmotion are registered trademaks of Eppendorf AG. Eppendorf Mastertip, Eppendorf Varitip P, Eppendorf Varitip S, Maxitip, Eppendorf Safe-LockTubes, 3810x, DNA + Protein LoBind Tubes, PCR Tubes, Cap Strips, Protein LoBind Deepwell Plates, Deepwell plates, DNA LoBind Deepwell Plates, Heat sealing Film and Foil, PCR, Storage Films and Foils, Plate Lid, GELoader and Microloader are trademaks of Eppendorf AG. Order No. AQ /GB1/5T/0812/FEEL/K&R All rights reserved, including graphics and images Copyright 2012 Eppendorf AG Printed climate neutrally in Germany