Breath Analysis Investigating the Human Volatome by ATD-GCMS

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1 Breath Analysis Investigating the Human Volatome by ATD-GCMS Presented by - Renee Cataneo Mayur Mundada Contributors Michael Phillips MD(Menssana Research Inc. CEO) Mark Libardoni PhD (Southwest Research Institute Staff Scientist, Space Science &Engineering) Pete Stevens PhD (3 degrees of separation Advanced Analytical Technologies) Joshua Whiting PhD (3 degrees of separation Advanced Analytical Technologies) Xiang Zhang PhD(University of Louisville, Kentucky) Menssana Research Inc. 2

2 List of Topics Menssana Research Inc. Breath Collection Technology (BCA) Technology choices we made I. BCA - GC/MS (quadrupole) II. BCA GCXGC-TOF MS LECO Pegasus 4D 3

3 Menssana Research Inc. Founded in early 1990 s by Dr. Michael Phillips. Menssana performs multicenter international clinical studies funded mostly by grants and contracts. Different technologies being tested at Menssana include I. Lab Based GC/MS (quadrupole), GCXGC-TOF MS II. POC GC-FID, GC-SAW and e-noze array technology 4

4 Breath Collection Technology Dr. Michael Phillips patented the Breath Collection Apparatus (BCA). BCA reservoir separates alveolar breath from the ambient dead space air. Concentrates sample onto collection tube. 5

5 Breath Collection Apparatus Collect 1 L of human breath sample and 1 L of ambient air sample. A 2 min. collection for each breath and air sample. Use multiple bed sorbent tubes to collect breath and air samples. Samples can be collected and stored in the freezer for months. 6

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7 Technology Choices We Made Started with GC-MS (quadrupole) in early 1990 s Chose carbon based adsorbent material and non-polar stationary phase. 8

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9 GC-MS (quadrupole) Human Breath Sample Chromatogram 10

10 Peak Purity in GC-MS 11

11 LECO Pegasus 4D GCXGC-TOF MS Advantages I. Sensitivity II. Less spectral skewing III. Orthogonal Separation, De-convolution software - better separation. Desorption system chosen was Markes Unity-2. Advantages are diffusion lock caps, sample splitting and recollection. 12

12 Markes Unity-2 Thermal Desorber and LECO Pegasus 4D GCXGC-TOFMS 13

13 LECO Pegasus 4D GCXGC-TOF MS Generated by LECO Corporation 14

14 LN 2 Dewar Arm LECO Pegasus 4D Modulator Cold Jets Orthogonal Hot Jets Second Dimension Column Generated by LECO Corporation

15 Method Development Samples were collected onto sorbent traps using BCA. 1 L samples were collected. Samples were desorbed using Markes Unity-2 desorber. Thanks to LECO team for assistance in method development! 16

16 Method Development Two columns and dual temperature ramp rates resulted better separation of VOCs. Shorter run time (35min). Modulator helps in refocusing of the compounds. TOF MS can scan up to 500 scans/second. Onboard de-convolution helps in better identification. 17

17 BCA GCXGC-TOF MS Human Breath Sample Chromatogram 18

18 3D View GCXGC-TOF MS Data for a Breath Sample 19

19 Zoomed In View Separation of Peaks in Two Chromatographic Dimensions 20

20 Breath Air Sample Chromatogram 21

21 Contour Plot Human Breath Sample Chromatogram 22

22 Compound Classification Generated by LECO Corporation 23

23 Some of the Abundant Compounds in Human Breath Name CAS Undecane, 2,6-dimethyl Styrene Nonane, 2-methyl Octane, 4-methyl Cyclohexane Furan, 2-methyl Propene, 1-(methylthio)-, (E) ,4-Dimethyl-1-heptene Benzene, 1-methyl-2-(1-methylethyl) methyl-1,3-butadiene (isoprene) Bicyclo[3.1.1]hept-2-ene, 2,6,6-trimethyl-, (ñ) Octanol, 2-butyl Propane, 1-(methylthio) Benzene, 1-methyl-4-(1-methylethenyl) Tetradecene, (E) Octane Dodecane, 4,6-dimethyl Furan, 2,5-dimethyl Tetradecane Heptane, 2,4-dimethyl Cyclohexene Undecane, 4,8-dimethyl

24 Diseases Studied Ongoing Projects I. Lung Cancer II. Breast Cancer III. Heart Transplant Rejection IV. Tuberculosis V. Influenza VI. Metabolic disorder Diabetes & Body Odor Environmental Toxicology I. Exposure to toxic VOCs II. Radiation bio dosimeter 25

25 Advantages GC - MS Conclusion 1. When coupled with pre-concentration allows us to see many components of human breath sample. Possible Improvements 1. De-convolution of co-eluted peaks. 2. Allowed us to use NIST library. 2. Better separation & identification. 3. We see approximately 200 VOC s. 3. Less spectral skewing. LECO Pegasus 4D GCXGC-TOF MS 1. Orthogonal separation. 1. Improve data alignment capability. 2. On board de-convolution software. 3. Less spectral skewing because of TOF MS, better sensitivity and resolution. 4. Possible to see different islands chemical types compounds. (alkanes, acids, benzenes etc) 5. We see approximately 2000 VOCs in human breath sample. 6. We can use NIST library. 26

26 Menssana s Goals LECO Pegasus 4D GCXGC-TOF MS is our favored technology for lab based biomarker discovery. Develop Point of Care technology based on biomarkers discovered. 27

27 Questions?? Renee Cataneo Mayur Mundada 28