Magnetic Resonance for Quaility Control of Palm Oil

Transcription

1 Magnetic Resonance for Quaility Control of Palm Oil

2 SFC Solid Fat Content

3 SFC NMR Solid Fat Content

4 Magnetic Resonance Overview MR CW-MR Pulse-MR High Res FT-MR High Low Resolution NMR Free NMR Induction Spectrum Decay Low Res TD-MR Industrial Organic Applications: Chemistry: Molecular Tyres, Toothpaste, Structure Elucidation Soaps etc. Food Bio Applications: Chemistry: Protein Palm Oil, Structures Seeds, Chips, and Dynamics Chocolate etc. Chemical Inorganic Industry: Chemistry: Polymers, Catalysis, Christalinity organo metalic Construction Material Science: Industry: Polymer Concrete Structures, Blends etc. Textile Physics: Industry: Quantum Spin Computing, Finnish Physical Chemistry many more.

5 SFC Solid Fat Content Time domain NMR Signal F S S+L Solid 0 S S+L S Liquid L t [µs] f - factor ( ) Standard- f - factor ( ) Special-Fats linear extrapolation to t = 0 September 4, 2013

6 SFC Solid Fat Content Time domain NMR TD-NMR Measurements Groups Absolute NMR Methods Ratio NMR Methods FID Hahn-Echo T1 NMR Relaxation Time T2 NMR Relaxation Time which allows to differentiate between 0% solids(100% liquid) and almost 100% solids(0% liquid)

7 SFC NMR Solid Fat Content

8 SFC Solid Fat Content Time domain NMR September 4, 2013

9 Structure MR a well known correlation

10 Structure MR a well known correlation We need pure, clean compounds

11 The Real World is a Mixture

12 Can Magnetic Resonance help in the Real World?

13 Why Magnetic Resonance? MR is linear: double sample amount => double signal 1H MR 4000 human samples MR vs. conv. creatinine measurement (Jaffe) Correlation ~ 0.95

14 Why Magnetic Resonance? MR is time invariant: sample measured today = sample measured tomorrow - NOESYGPPR1D - automatic phasing - no baseline correction! 1.2Hz Automated preparation, transfer, measurement and processing of multiple replicate samples

15 Why Magnetic Resonance? KEY STATEMENT MR is SOFT Magnetic Radio Wave shines on sample No Ionization No destruction of sample can repeat without loss or change of signal CAN DETERMINE STABILITY OF MEASUREMENT Variations outside the measurement fluctuations must be caused by chemical differences

16 What is Palm Oil? Production Mio Met Ton

17 What is Palm Oil?

18 What is the Quality of Palm Oil? Basic Methods

19 What is the Quality of Palm Oil? Advanced methods: SFC Solid Fat Content DOBI Deterioration Of Bleachability Index IV Iodine Value FFA Free Fatty Acid Content MAG Mono-acylglycerols DAG Di-acylglycerols DAG

20 Example DOBI Deterioration Of Bleachability Index

21 Example DOBI Deterioration Of Bleachability Index

22 Example IV Iodine Value Degree of Unsaturation

23 Example FFA Free Fatty Acid Content

24 Magnetic Resonance We know it already

25 Magnetic Resonance Imaging the molecules

26 Example IV Iodine Value

27 Example CPO - RBD Chem. Dept. June Malaysia

28 Example CPO - RBD Chem. Dept. June Malaysia

29 Example CPO - RBD Chem. Dept. June Malaysia 2 samples measured 10x

30 Example CPO - RBD Chem. Dept. June Malaysia

31 Testing with ONE Magnetic Resonance System Check it out:

32 Testing with ONE Magnetic Resonance System

33 Palm Oil From The Store measured MR 500 MHz PTT Wang Noi

34 Palm Oil From The Store measured MR 500 MHz PTT Wang Noi

35 Palm Oil From The Store measured MR 500 MHz PTT Wang Noi

36 Determination of geographical origin of olive oils by MR

37 Olive Oil LDA with Prediction Italy versus Greek Islands Cooperation Bari University

38 Regional Discrimination Italy / Greek Islands Olive Oil DAUNO = it-1 15 samples Terra di Bari = it-2 31 samples Terra di Otranto = it-3 11 samples Kefalonia = gr-1 14 samples Korfu = gr-2 10 samples Lefkada = gr-3 8 samples Zakynthos = gr-4 14 samples Mean correct prediction rate: 78.2% (±2.6% standard deviation) Only ZG: 74%, Only NOESY: 65% Experiments used: ZG: ppm, 450 buckets NOESYGPPR1DSP: ppm, 450 buckets CDCl3 excluded ( ) No Scaling = Absolute concentration Per bucket: Unit variance scaling PCA Fixed Dimension: 50 Cross Validation: 7 sets Monte Carlo: 1000 runs

39 Wastewater from Palm Oil Mills? Can we predict Wasterwater products?

40 Energy Drinks

41 Energy Drinks Wikipedia says: Energy drinks are beverages whose producers advertise that they "boost energy". These advertisements usually do not emphasize energy derived from the sugar and caffeine they contain [1] but rather increased energy release due to a variety of stimulants and vitamins. [2]

42 Energy Drinks Thailand Krating Daeng M-150 Shark Red Bull 357 Thailand number One Energy country Thai Market $500Mio

43 Energy Drinks Thailand

44 Energy Drinks Thailand

45 Energy Drinks Thailand 2 x 106 PCA, 2D: 100 NMR measurements 1.5 Shark KratingDaeng LipoPlus Chalam Lipo 0 RedBull -0.5 Ranger -1 KalabauDeang M x 10 6

46 Magnetic Resonance for Characterization, Applied Chemistry, QC

47 Conclusion MR is cool because: Soft excitation of sample Repeatability Knowledge of measurement Linear double amount > double signal Time invariant Stability Reliability MR is not cool because: Detection limit ppm rather than ppb Difficult for trace analysis Need 3 rd generation systems only new electronics (e.g. low noise shims, RF pulses etc.) can do Expensive maybe. BUT FOURIER. 47

48 Conclusion Magnetic Resonance enables innovation Competitive advantage with new approach to analyze Prevent Chinese additives in your product Clean, Nondestructive and sexy approach to QC

49 49 MR is affordable

50 50 It s here already and

51 51 It s here already Assure Software:

52 Magnetic Resonance for Characterization, Applied Chemistry, QC Thank You: Gabi Rupprecht, Bruker Switzerland Willy Schaerer, Bruker Switzerland Azhar Ariffin, Universiti Malaya, Kuala Lumpur, Malaysia Nordin Bin Mohamed, Universiti Malaya, Kuala Lumpur, Malaysia Fang Fang, Bruker Germany Supaporn Khaubunsongserm, PTT R&D, Wang Noi, Thailand 52

53 ??? Confused??? Anyway thank you for listening 53