Supporting Information. Ultrasensitive and facile detection of microrna via portable. pressure meter

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1 Supporting Information Ultrasensitive and facile detection of microrna via portable pressure meter Lu Shi a, Jing Lei a, Bei Zhang a, Baoxin Li a, Chaoyong James Yang b and Yan Jin a, * a Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi an , China b State Key Laboratory of Physical Chemistry of Solid Surfaces, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen , China * Corresponding author. Tel: ; Fax: ; jinyan@snnu.edu.cn S-1

2 Figure S1. (a) Gas pressure of MB/H 2 O 2 mixture with the prolongation of reaction time. The concentration of MBs is 0.5 mg ml -1. (b) Gas pressure of MB/H 2 O 2 mixture with the increasing of the concentration of MBs. S-2

3 Figure S2. (a and b) TEM images of PtNPs. (c) Pressure-change with different concentration of H2O2 at different reaction time. (d) Linear relationship between pressure value and different concentrations of PtNPs. S-3

4 Figure S3. Reproducibility of pressure measurements. The concentration of H 2 O 2 and PtNPs are 10 M and 8 nm. S-4

5 Figure S4 Influences of experimental parameters on the analytical performance: (a) Effect of the concentration of MBs, (b) Effect of reaction time between MBs and DNA H1, (c) Effect of buffer, (d) Effect of the concentration of SSC buffer. S-5

6 Figure S5 Influences of experimental parameters on the analytical performance: (a and b) effect of the concentration ration of H1 and H2, (c and d) effect of reaction time of SDR, (e and f) effect of reaction temperature of SDR. S-6

7 Table S1. Pressure change at different reaction time. Group Pressure after 3 h Pressure after 6 h Reduction in 3 h kpa 70.5 kpa 2.8% kpa 76.0 kpa 3.3% kpa 74.1 kpa 2.1% S-7

8 Table S2.Comparision of the analytical performance of different methods for mirna detection. Analtical Method Target Linear range Detection limit Detection media refs Fluorometric assay mir nm 100 pm Human serum 1 Fluorometric assay let-7a 100 fm-1 nm 58 fm Tris-acetate 2 Fluorometric assay mir nm 47 pm Total RNA 3 Electrochemical assay mir-182-5p pm 0.5 fm PBS buffer 4 Electrochemical assay mir pm Total RNA 5 Electrochemical assay mir fm-5 pm 10 fm Total RNA Paient samples 6 Surface-enhanced Raman scattering assay mir-21 1 pm-10 nm 1 pm PBS buffer Serum 7 nanoparticle amplification method mir pm Total RNA 8 Colorimetric assay mir pm-3 nm 70 fm Total RNA 9 PGM-based assay mir nm 190 pm Total RNA 10 Pressure-based assay mir fm-10 pm 7.6 fm Total RNA Human serum This work References (1) Zhang, J.; Zhao, Q.; Wu, Y. D.; Zhang, B.; Peng, W. P.; Piao, J. F.; Zhou, Y. R.; Gao, W. C.; Gong, X. Q.; Chang, J. The construction of a novel nucleic acids detection microplatform based on the NSET for one-step detecting TK1-DNA and microrna-21. Biosens. Bioelectron. 2017, 97, (2) Wang, L. D.; Deng, R. J.; Li J. H. Target-fueled DNA walker for highly selective mirna detection. Chem. Sci. 2015, 6, S-8

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