Why this seminar? Verhütung von Unfällen und Berufskrankheiten. The employer has to take security measures!

Transcription

1 LAB SAFETY

2 Why this seminar? Obligationenrecht Verhütung von Unfällen und Berufskrankheiten Arbeitnehmerschutz The employer has to take security measures according to best available technology adequate necessity (based on experience) The employer must consult and involve the employees OR UVG ArG

3 Who are we? Daniel Bopp 11-J Werner Boll 55-K-44a Erich Frei 55-K-34a Silvio Hemmi 32-J General Safety Chemical Safety Radiation Safety Biological Safety

4 Your Duties

5 follow directives of employer respect/obey safety rules proper use of safety installations Employee use PPE repair defects or report attend medical check-ups work in sober condition (alcohol, drugs)

6 Safety is everyone s concern and responsibility Verordnung 3 zum Arbeitsgesetz (ArGV 3) Verordnung über die Verhütung von Unfällen und Berufskrankheiten (VUV)

7 NO eating, NO drinking NO smoking NO applying of makeup

8 - keep your bench clean - dispose of garbage - test equipment (repair or discharge when defective) - correct storage of chemicals - label drawers and cupboards

9 - Handling of chemicals - Working with isotopes - Proper use of equipment - Personal protective equipment - Fire - Medical attendance

10 hazardous handling of chemicals EU-conform pictograms R or H code: risks or hazards statements S or P code: safety or precautionary statements

11 hazardous example: chemicals HCl corrosive R 34 causes etching R 35 causes severe etching R 36 irritates eyes. R 37 irritates respiratory system. S 26 when in contact with eyes thoroughly flush with water and consult medical personell. S 27 when in contact with clothes, remove soaked clothes. S 28 when in contact with skin wash with lots of water S 29 dont flush in canalisation S 30 never add water (extreme exothermic process) etc.

12 hazardous chemicals read safety sheets (should be included) label fridges or cabinet containing hazardous compounds

13 hazard symbols I - highly poisonous e.g. cyanids, mercurychlorid - poisonous e.g. formaldehyde, formamide, TCA - corrosive e.g. concentrated acids and alkali - health hazard e.g. SDS, paraformaldehyde - irritant e.g. hypochlorite, soda, diverse cleaning agents - environmental hazard e.g. lead compounds

14 Toxicity LD50 * Ethidiumbromid 1503 mg/kg KG Acrylamid mg/kg KG Natriumazid 20 mg/kg KG Formaldehyd 270 mg/kg KG (dermal) Phenol mg/kg KG (oral) Koffein 100 mg/kg KG Koffein 100 mg/kg KG Nikotin 5 mg/kg KG

15 hazard symbols II - inflammable e.g. petrol, various alcohols - highly inflammable e.g. hydrogen, acetylen - explosive e.g. hydrogenperoxid - oxidizing e.g. hydrogenperoxid

16 Storage of chemicals

17 use & disposal ethidium bromide: gloves collect solid waste in specified container pass liquid waste through extractor hydrochloric acid: gloves, glasses, lab coat store in acid-resistant cabinet under the hood dilute down the sink phenol gloves, glasses, lab coat store under the hood collect into waste containers

18 transport of chemicals 0-5 kg : Use baskets. Can be obtained in the shop Y12-D kg : Use a trolley with a catch basin. Provided by Department Sicherheit und Umwelt. > 40 kg : Hazardous material can only be transported after consultation with Department of Sicherheit und Umwelt. liquid nitrogen or solid CO2 (> 5kg) should NEVER be transported in elevators 1 kg solid CO2 produces 0.5 m 3 CO2 gas 1 liter liquid nitrogen produces 0.7 m 3 N2 gas

19 working with isotopes C-LAB 32-J-38

20 Registration and Instruction All persons intending to work with radioactive isotopes and the Seifert X-Ray equipment at our institute must register with the radiation safety expert IMLS West: Daniel Bopp deputy IMLS Ost: Werner Boll It is the responsibility of the group leaders to make sure, that only registered and instructed members of their group are working with radioactive compounds and the X-Ray equipment. Persons leaving the Institute should inform the radiation safety expert, return any dosimeters and provide a forwarding address for the record sheet if they plan to continue to work with radioactivity.

21 Access C-LAB 32-J-38 chip card IMLS West card in 11-J-70 lab, ask Daniel or Claudia IMLS East card in 55-K-44a, ask Werner Always sign in for taking and returning card

22 Dosimetry All persons working with strong beta-emitters and the X-Ray equipment will be issued with a TLD-badge for monitoring radiation. The badge must be attached to the outside of the lab coat. Badges are evaluated monthly by SUVA and the exposure is entered into your records ( Yellow leaflet, Schweizerisches persönliches Dosisdokument, column Hp resp. Hs). Please inform Bettina Cardel (55396) where you keep your badge. She will exchange the badges for evaluation once a month. In addition to the dosimetry, all persons professionally exposed to radiation have to undergo a medical entry examination. Contact Bettina for obtaining the appropriate forms

23 RULES FOR THE C-LAB Before working in the C-lab write the following into the list (on the door) your name the date the isotope 35 S, 3 H, 32 P or 14 C the estimated amount you used in mci

24 in case of an serious accident with radioactive contamination, proceed as stated below ALARM Call number 175 (Betriebsdienst Irchel) RESCUE Move injured persons out of the contaminated area. Call number 112 for emergencies. CLOSE OFF CONTAMINATED AREA Call number 175 to coordinate the measures that have to be taken

25 Important Phone numbers Jörg Frank Stab Sicherheit und Umwelt Stefan Brentari Waste disposal isotopes Daniel Bopp radiation safety expert IMLS West Werner Boll radiation safety expert IMLS Ost Bettina Cardel Dosimetry and medical check-ups 55396

26 Proper use of equipment UV-transilluminator: use glasses protective shield autoclave do not use the autoclave without being instructed

27 pipettes NEVER pipet by mouth ultrasonic-lab equipment use only with ear protection +

28 Gelelectrophoresis cover with lid danger of electric shock

29 Lasers Some research labs have high powered lasers (class 3 and 4). These labs are restricted areas for access by authorised persons only (e.g. 32-J-78a) Staff and students in these areas must be instructed before commencing work.

30 General Working Practice Use Personal Protective Equipment

31 Protective Clothing I Safety Glasses A must when handling acidic, alkalic or other irritant chemicals (labeled with S39) Must also be worn when handling radioactive material.

32 Protective Clothing II Gloves When handling hazardous chemicals wear rubber disposable gloves (a must when labeled with S 37). Remove and dispose of them before you leave the lab or answer the telephone. When handling radioactive material

33 Protective Clothing III Lab coats Cotton laboratory coats must be worn when working with corrosive chemicals. If spilled, it is easier to take off a lab coat than taking off your clothes. (And are cheaper to replace.) Also protects you from flash burns. a must when working in the C-lab

34 First aid first-aid box present in every laboratory band aid, disinfectant acid, alkali burns in the eye, flush immediately with lots of water

35 Emergency Medical Services CALL 112 intern or 144 extern

36 Fire If you discover a fire In all cases, set off the fire alarms via break glass panel or call 112. This will summon the rescue team and the fire service, and alert others to evacuate the building. Go to the control point outside of 23-H (next to the fountain) and inform the fire service about the situation in the building.

37 Fire

38 last but not least... pregnancy: try to refrain from working with irritating chemicals and from being exposed to ionizing radiation. Inform your supervisor trainees: if you supervise the lab work of a trainee (e.g. summer student), inform him/her about potential hazards and how to properly work with lab material and equipment

39 Information documentation can be found on IMLS (services)

40 Information more documentations can be found on

41 Safety is everyone s concern and responsibility