Environment Department CoSHH Assessment Form

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1 Environment Department CoSHH Assessment Form This document should be completed electronically or in handwritten block capitals and by consulting current Safety Data Sheets (SDS). Completed forms should be assessed by the Project Supervisor and reviewed by Dave Hay (Safety Officer). You should return a signed copy to Dave Hay and send a pdf. copy to a member of the Technical Staff to be added to the Dept. electronic records. LOCATION AND PERSONNEL Department: Environment Researcher: Matt Pickering Location of work (room numbers): Chromatography lab ENV/114 Research Supervisor: Title of Proposed work: General use of the high performance liquid chromatograph (HPLC) UNIVERSITY HAZARD SEVERITY LEVEL (see Appendix) Substances used/formed during the experiment Approx. quantities Haz. code Specific H-statements including text descriptions (see SDS) Methanol (HPLC grade) 2.5 L 2 H225 - Highly flammable liquid and vapour. H301, H311, H331 - Toxic if swallowed, in contact with skin or inhaled. H370 - Causes damage to organs. Acetonitrile (HPLC grade) 2.5 L 2 H225 - Highly flammable liquid and vapour. H302, H312, H332 Hazardous if swallowed, in contact with skin or inhaled. H319 - Causes serious eye irritation. Ethyl acetate (HPLC grade) 2.5 L 2 H225 - Highly flammable liquid and vapour. H319 - Causes serious eye irritation. H336 - May cause drowsiness or dizziness. Formic acid 10 ml 2 H226 - Flammable liquid and vapour. H302 - Harmful if swallowed. H331 - Toxic if inhaled. Acetic Acid 10 ml 2 H226 - Flammable liquid and vapour. Ammonia solution 10 ml 2 H225 - Highly flammable liquid and vapour. H301, H311, H331 - Toxic if swallowed, in contact with skin or inhaled. H370 - Causes damage to organs. H411 - Toxic to aquatic life with long lasting effects. Ammonium formate 5 g 1 Not a hazardous substance according to EC Ammonium acetate 5 g 1 Not a hazardous substance according to EC Phosphoric acid 10 ml 2 H290 - May be corrosive to metals. Ammonium phosphate 5 g 1 Not a hazardous substance according to EC monobasic Ammonium phosphate dibasic 5 g 1 Not a hazardous substance according to EC Page 1 of 5

2 SECTION 1 ASSESSMENT AND REVIEW GENERAL HANDLING CONTROL MEASURES Using the Precautionary (P-) Statements and additional information listed in the SDS as a guide, detail the control measures to be adopted: Wear gloves, lab spectacles and a lab coat when handling these chemicals. Perform all work in a fume hood with the exception of weighing ammonium formate, ammonium acetate or ammonium phosphate and attaching solvents to the HPLC instrument. Keep flammable solvents, methanol, acetonitrile, ethyl acetate, acetic acid, formic acid and ammonia solution away from hot surfaces, sparks, open flames and other sources of ignition. EMERGENCY HANDLING CONTROL MEASURES Using the Precautionary (P-) Statements and additional information listed in the SDS as a guide, detail the actions to be adopted in the event of an emergency (e.g. spillage, exposure to personnel): Exposure to personnel If INHALED remove to fresh air and call a doctor. In case of CONTACT WITH SKIN wash affected area with cold water. In case of CONTACT WITH EYES, irrigate with large volumes of water. Remove contact lenses if present and easy to do so and continue rinsing. Call a doctor. Spillage SMALL SPILL - clean up with blue roll, washing any surfaces with water afterwards, and dispose of cleaning materials in the yellow Tiger bags provided in the lab. LARGE CONTAINED SPILL (such as in a fume cupboard or solvent cabinet) clean up with the chemical spill kit provided in the lab and dispose of all cleaning materials in the yellow plastic waste sack. Avoid breathing fumes/vapours. LARGE UNCONTAINED SPILL of a flammable or hazardous substance, activate the fire alarm and immediately evacuate the building. Once at the fire assemble point, report the incident to the Dave Hay or one of the lab technicians. ADDITIONAL INFORMATION Any other information not covered by COSHH e.g. flammability, heat, cold, noise, pressure: SECTION 2 ASSESSMENT AND REVIEW Page 2 of 5

3 PROCEDURE Give details of the proposed procedure: Preparation of solvents for use with the HPLC Pure solvents Pure solvents will be decanted from 2.5 L Winchester bottles into Duran-style bottles. Buffer solutions Ammonium acetate, ammonium formate or ammonium phosphate will be weighed out, transferred to a 1 L volumetric flask and made up to the mark with water (HPLC grade) to produce solutions with a final concentration of approximately 50 mm or below. The ph of the solution may be adjusted by addition of small amounts of acetic acid, formic acid, phosphoric acid or ammonia solution as appropriate. Solutions will be filtered through glass fibre filters before transferring to a Duran-style bottle. Solutions of formic acid 1% (v/v) solutions of formic acid will be prepared from addition of concentrated formic acid to water (HPLC grade), acetonitrile or methanol. Attaching solvents to the HPLC Lab spectacles should be worn when connecting/disconnecting solvent reservoir bottles on the HPLC. DISPOSAL AND STORAGE PROCEDURES Give details of the proposed disposal and storage procedures, including storage conditions and approximate expiry times of samples/reagents prepared: Storage - Store flammable solvents, methanol, acetonitrile and ethyl acetate, in yellow steel flammable cabinets. The only exception is for solvent bottles connected directly to the HPLC. Store pure formic acid and acetic acid in a corrosives cabinet with other organic acids and separately from inorganic acids and bases. Solutions of formic acid should be stored according to the nature of the solvent. Ammonia solution should be stored in a corrosive cabinet and separately from acids. Solutions of ammonium formate or ammonium acetate should be stored according to the nature of the solvent. Waste - Flammable solvent and mixed HPLC eluent waste should be disposed of as flammable waste in glass or plastic bottles. Dilute solutions of ammonium acetate, ammonium formate or ammonium phosphate in water may be disposed of down the sink accompanied by plenty of water. SECTION 3 ASSESSMENT AND REVIEW Page 3 of 5

4 Appendix 1 Hazard code 1: Chemicals Non-hazardous University Chemical Hazard Codes This category is allocated to a chemical substances classified as non-hazardous by the chemical supplier. The label or safety data sheet (SDS) contains no hazard warning words, symbols or phases. There is no action or restriction when ordering via the York e Purchase (YEP) System. Hazard code 2: Chemicals - Hazardous A chemical substance that is classed as hazardous but is not a high hazard chemical (see Hazard code 3). The label or SDS includes hazardous words, symbols or phases but no high hazard words (see Appendix 2). There is no action or restriction when ordering via YEP unless a chemical substance is of a higher hazardous classification. Hazard code 3: Chemicals - Hazardous (High) A chemical substance that capable of causing serious harm if exposure occurred. The label or SDS includes one or more high hazard words, symbols or phases (see Appendix 2). There are management restrictions which require approval/authorisation when ordering via YEP. Hazard code 4: Chemicals - Hazardous (legally controlled) A chemical substance that is legally controlled and requires approval/authorisation before ordering. The label or SDS includes one or more high hazard words, symbols or phases (see Appendix 2). Legally controlled substances include: Drugs of abuse Drugs precursors schedule 1 Explosives requiring license Schedules 1, 2 (chemical weapons and precursors) Schedule 3 (precursors) There are management restrictions which require an approval/authorisation by the license holder and Head of Department when ordering via YEP. Page 4 of 5

5 Appendix 2 High hazard words, symbols and phases Examples of chemical substance characteristics that are allocated to this group may include, but are not limited to: H200 H201 Hazard statement Symbol Signal word Unstable explosive Explosive; mass explosion hazard H202 H203 H240 Explosive; severe projection hazard Explosive; fire, blast or projection hazard Heating may cause an explosion H250 Catches fire spontaneously if exposed to air H300 Fatal if swallowed H310 Fatal in contact with skin H330 Fatal if inhaled H340 May cause genetic defects H350 May cause cancer H360 May damage fertility or the unborn child Page 5 of 5

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