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6 4. Influence sarde myibrils (p As shown There 7.7 SDS-PAGE 2 days. KC1 plus (B) protes irrespective ly prepari. higher. ordary 35 Ž than The as ptern Sarde 50mm ordary phosphe prote ( ) buffer myibrils were ordary ry low any Their he 7 immediemyibrils, varied markedly acidic 6.7, ctrol ( 7), subjected ( ) n SDS-PAGE p regi though still much immediely decreased The myos ma pomyos de- acmyos. prote On clearly rise b even The also probably such came th from h, It. s exhibited b or m. dissocied observed cha prepari. Act which 35 Ž than 6 show prote maximum lower heavy rement. cha subsequent pterns cubi a phographs electrophoretic 6. The ordary. 30m, 68% -20 Ž. 85% high electrophoretic from shown respec- any decreased In 83%, s maximum srage change prote 0 Ž 0.5M 79% myibrils cud sarde frozen apparent pendg (A) ordary showed solubilized both myibrillar ptern 5, days no When 40% 2 myibrils ptern srage phographs). tively, were prote myibrils hardly a mild f. tro- aggreged myos orda- heavy prote

7 5. Influence sarde Refer acidic ordary (A, ƒ ) legend solublity 4 myibrils, SDS-PAGE (B, ) furr especially Mg2+- sarde durg en fibrils srage might teracti myos At absence ice loss srage, due by moiety. 0 Ž, After frozen hardly g Ca2+. ed Evidence an modifici th durg frozen both seen were discussi hydrogen not might tributable myibrillar protes decreased medium act-myos groups observed result affity by hydrogen tween Ohnishi acmyos bds et al.10) durg cmial frozen, creased tween ag- as found by myoss. This would act un emphasiz- resulted structural which clearly must probably myibrillar protes rabbit trout it an teracti gregi Buttkus10) en neutral Untune-, however, bd srage, system. It should, acidic assay any showed alkale pre- 2 days. myibrils hydrophobic myibrils alkale thiol well -20 Ž has Ca-sensitivity oxidi have myo- prote phenomena thus st speculive. sarde 9 both present myibrils remag around especially srage, remag dersod, values both th maximum elsewhere7) various alkale presented durg exhibited demstred. sence ordary maximum ly, srage any Mg2+- Discussi Changes details. regi. ptern myibrils result myos carp a as ordary srage. As teracti a

8 6. Influence s arde Refer myos ree Mg2+ assay ordary legend proporti ly high argued th 4 furr It both due ca- a comparive- reacti might is dissocii due regulory cubi by presence or absence Ca2+ a mild he trement could due cmial occurrg regulory protes troptropomyos complex. It would seem n, least case sarde, th Ca2+ - et prote 35 Ž 30m durg. durg general srage higher Secdly, proment various acmyos 35 Ž myos 7 could th act compared. ordary s At fishes myibrillar 35 those first, or made - values reported both can Mg2+ than those crease fish observis quite agreed acmyos. trement. myibrillar found flyg tem- acmyos skeletal tween as frog th cubi more ccerng well range teracti rmal Four 30 Ž reported 50m due functi rabbit al.22) - not physiological et durg al.21) Ž Taguchi does above Fuchs The crease Mg2+- loss Ca-sensitivity both myibrils eir system perures Mg2+ protes where readily occurs moderely high net negive charge macromolecules.20) is ptern myibrils details. reduced. myibrils SDS-PAGE an alkale reversible (B, œ) neutral However, polymerizi partially might acidic ale. decreased by myos labile (A, ) act th systems an creased lyzed likely ordary. This.23-25) -20 Ž ice were srage.

9 Thirdly, low 5.5 sarde ordary myibrils revealed direct functi. Fally, it should noted th most proment differences exist dependency tween remag Ca2+-3) Mg2+- activities both sarde ordary myibrils when sred -20 Ž. The maximum remag Ca2+- observed a level around 7.5, while extremely low Mg2+- this regi irrespective type. Theree, it can ccluded th myibrillar Mg2+- is a sensitive parameter know myibrillar denuri durg frozen srage neutral regi. Unlike Mg2+-, re no marked change electrophoretic ptern any both myibrils sred eir 0 or -20 Ž. Although ice zen srage quite high throughout examed, it is probable th might decrease extended srage period as found by Seki et al.7) carp myibrils durg ice srage Oguni et al.26) carp acmyos durg frozen srage. The ordary myibrils decreased low 40% any durg he trement 35 Ž 30 m. The result csistent SDS-PAGE ptern which showed no myos heavy cha prote throughout range examed. Some types aggreged acmyos which is not salt- would rar active Mg2+-. The myibrils also csiderably decreased but remaed much higher weakly acidic neutral ranges than alkale range. This well correspded SDS-PAGE pterns prote where less amount myos heavy cha observed above 7.5. Acknowledgments We are greful staff Kanagawa Prefectural Fisheries Experimental Sti ir help collectg sarde specimens. References 1) S. Wa, K. Kanna, T. Suzuki: Nipp Suisan Gakkaishi, 43, 1353 (1977). 2) K. Hashimo, S. Wa, M. Ko, K. Shiro: Nipp Suisan Gakkaishi, 45, (1979). 3) S. Wa, M. Kamal, K. Hashimo: Nipp Suisan Gakkaishi, 55, (1989). 4) M. Burke, E. Reisler, W. F. Harrgn: Proc. N. Acad. Sci., 70, (1973). 5) E. Reisler, M. Burke, W. F. Harrgn: Biochemistry, 13, (1974). 6) S. Ebashi M. Endo: Prog. Biophys. Mol. Biol., 18, 123 (1968). 7) N. Seki, M. Ikeda, N. Narita: Nipp Suisan Gakkaishi, 45, (1979). 8) N. Seki, Y. Oogane, T. Wana: Nipp Suisan Gakkaishi, 46, (1980). 9) S. Noguchi J. J. Msumo: Nipp Suisa n Gakkaishi, 45, (1970). 10) H. Buttkus: J. Food Sci., 35, (1970). 11) K. Arai R. Takashi: Nipp Suisan Gakkaishi, 39, (1973). 12) Y. Fukuda, Z. Tarakita, M. Kawamura, K. Kakeha, K. Arai: Nipp Suisan Gakkaishi, 48, (1982). 13) Y. Fukuda, Z. Tarakita, K. Arai: Nipp Suisan Gakkaishi, 50, (1984). 14) T. Suzuki S. Wa: Food Rev. Intern., 2, ( ). 15) S. V. Perry T. C. Grey: Biochem. J., 64, (1956). 16) C. H. Fiske Y. SubbaRow: J. Biol. Chem., 66, (1925). 17) A. G. Gornall, C. J. Bardawill, M. M. David: J. Biol. Chem., 177, (1949). 18) U. K. Laemmli: Nure, 227, (1970).19) M. Ohnishi, T. Tsuchiya, J. J. Msumo: Nipp Suisan Gakkaishi, 44, (1978). 20) D. W. Frederiksen A. Holtzer: Biochemistry, 7, (1968). 21) F. Fuchs, D. J. Hartshorne, E. M. Barns: Comp. Biochem. Physiol., 51B, (1974). 22) T. Taguchi, M. Tanaka, Y. Nagashima, K. Amano: J. Food Sci., 51, (1986). 23) S. Wa, J. Maruyama, K. Hashimo: Nipp Suisan Gakkaishi, 49, 655 (1983). 24) I. A. Johnsn, N. Frears, G. Goldspk: Experientia, 28, (1972). 25) I. A. Johnsn B. Tota: Comp. Biochem. Physiol., 49B, (1974). 26) M. Oguni, T. Kubo, J. J. Msumo: Nipp Suisan Gakkaishi, 41, (1975). Nipp Suisan Gakkaishi: Formerly Bull. Japan. Soc. Sci. Fish.