Voltammetric Study Of Sildenafil Citrate Using Glassy Carbon Electrode
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1 ITERATIAL JURAL F TECHLGY EHACEMETS AD EMERGIG EGIEERIG RESEARCH, VL 2, ISSUE Voltmmtri Stuy f Silnfil Citrt Using Glssy Cron Eltro Tkly Mzg, Assf Srgwi Collg of nturl n omputtionl sins, prtmnt of hmistry, Aigrt Univrsity, Aigrt, Ethiopi, P..Box Collg of nturl n omputtionl sins, prtmnt of hmistry, Ais A Inistitut Thnology Univrsity, Ais A, Ethiopi Emil: kirostkly@gmil.om, sfsrg@yhoo.om Astrt: Th voltmmtri stuy of silnfil itrt hs n stui on glssy ron ltro using yli voltmmtry n iffrntil puls voltmmtry mtho. Silnfil itrt givs singl irrvrsil oxition wv ovr th wi ph rng stui. Using iffrntil puls voltmmtry n yli voltmmtry, silnfil itrt yil wll fin voltmmtri rspons in Britton-Roinson uffr solution, ph 3.26 t mv vrss Ag/AgCl. Ky wors: Vigr, GCE, CV 1. Introution 1.1. Gnrl kgroun Silnfil itrt (Vigr) is rug usful for trting ml rtil ysfuntion (ED). 1- It is lso fftiv for trtmnt of pulmonry rtril hyprtnsion (PAH). 6 Th rls of this rug hv vry lrg impt. This ws u to th ft tht th rug ws rkthrough for mn suffring from ED. Thy rprsnt signifint prt of th ml popultion : it is stimt tht % of mn suffr from rtil ysfuntion, n s muh s 2% for mn twn 4 n 7 yrs ol. 7 In 199, th rug Vigr ws introu s n fftiv wy to trt ml rtil ysfuntion. It gos y th gnri nm of silnfil itrt. It hs quikly om on of th most populr n most prsri rugs in th worl. Silnfil itrt ws synthsiz y group of phrmutil hmists working t Pfizr s rsrh fility in Engln. It ws isovr y int whil ttmpting to prou rug to trt oronry hrt iss. It m th first orl trtmnt pt y th Foo n Drug Aministrtion (FDA) to trt MED. Silnfil itrt is signt hmilly s 1-[[3-(6,7-ihyro-1-mthyl- 7-oxo-3-propyl-1H-pyrzolo[4,3-] pyrimiin--yl)-4- thoxyphnyl] sulfonyl]-4-mthylpiprzin itrt n hs th following struturl 9, formul. H 3 C H - H H S H Chmil formul: C 2 H S Molulr wight: g mol H CH 3 CH 3 CH 2 CH 2 CH 3 Figur 1.1. Th hmil strutur of silnfil itrt 1.2. jtivs of th stuy Gnrl jtiv Th gnrl ojtiv of this thsis is to stuy th voltmmtri hvior of silnfil itrt. Spifi jtiv To hk ph pnn of th oxition proprty of silnfil itrt To stuy th oxition proprty of silnfil itrt t iffrnt sn rt To xmin th oxition proprty of silnfil itrt using CV n DPV 2. Exprimntl prt 2.1. Chmils n Rgnts All rgnts r of nlytil gr n th solutions wr prpr using istill wtr. Th following hmils wr us throughout th stuy; Mthnol (Blulux) Silnfil itrt (Vigr) Ati i (Blulux), ori i (Blulux) n - phosphori i (Blulux) Soium hyroxi (Blulux) Th working ltro ws prtrt y polishing it with luminium oxi powr n rins in istill wtr Apprtus Th voltmmtri xprimnts wr prform using th BAS B, ltrohmil nlyzr [Bionlytil systms (BAS), USA], whih ws onnt to omputr systm. Th ph of th uffr solution ws msur with Jnwy instrumnts igitl ph mtr with omintion glss ltro. Mss of soli rgnts ws msur using Dnvr instrumnt ln. All th p otntils r trmin with rspt to Ag/AgCl rfrn ltro Eltrohmil lls n Eltros This work ws prform using thr ltro systm with on-omprtmnt glss voltmmtri ll, Ag/AgCl ltro s rfrn ltro n pltinum wir s n uxiliry ltro. Th working ltro is glssy ron ltro (GCE). Copyright 14 IJTEEE.
2 ITERATIAL JURAL F TECHLGY EHACEMETS AD EMERGIG EGIEERIG RESEARCH, VL 2, ISSUE Prour Figur 4.1. Complt voltmmtri ll stn Prprtion of stnr solution n Buffr solution For oth yli n iffrntil puls voltmmtri stuy, M of silnfil itrt stok solution ws prpr y issolving.1grm of silnfil itrt in ml of mthnol..4 M of Briton-Roinson uffr ws prpr y mixing th rquir mount of Ati i, Bori i n -phosphori i n th ph ws just y ing rops of soium hyroxi. Sril ilution of th stok solution ws m with quous uffr solution to otin th working solutions from M to M for CV nlysis n M to M for DPV nlysis. 3. Rsult n Disussion In this ppr th ltrohmil oxition of silnfil itrt hs n stui using yli voltmmtry n iffrntil puls voltmmtry. Th optimum ph n to stuy th ltrohmil hvior of this ompoun using th ov mntion ltronlytil thniqus ws ph Eltrohmil Bhvior of silnfil itrt in Cyli Voltmmtry Th Cyli Voltmmogrm of silnfil itrt Th ltrohmil hvior of silnfil itrt ws stui using yli voltmmtry t trt GCE. It shows on irrvrsil oxition pk t out 1.23 V s shown in Figur 3.1 low. Th otin pk orrspons to th oxition of piprzin ring.. Efft of ph Th influn of ph on pk urrnt of silnfil itrt hs n stui in th ph rng of Whn th ph of th supporting ltrolyt is inrss, th pk urrnt of th voltmmogrms is shift to mor ngtiv potntil. Th pk urrnt otin in uffr of.1 is muh lss thn tht otin for th uffr solution of ph Figur 3.2 shows Cyli voltmmogrm of M silnfil itrt t iffrnt ph rng in.4 M Briton-Roinson uffr. Th mximum pk urrnt ws osrv t ph Th pk urrnt is low t high ph rngs n strts inrsing s th ph rss n rhs mximum vlu t ph As shown in Figur 3.3 th pk potntil ws lso vri s th ph of uffr vris. Pk urrnt(µa) P k urrn t (µ A ) ph Pk potntil(v) Figur 3.2. Cyli voltmmogrm of M Silnfil itrt t iffrnt ph rng (. 1.1,. 3.26,. 4.1,..1) in.4 M Briton-Roinson uffr t sn rt of mv/s Inst: Plot of pk urrnt vrsus ph Pk potntil (V) Figur 3.1 Cyli voltmmogrm of M silnfil itrt t GCE in.4 M Briton-Roinson uffr (ph = 3.26) t sn rt of mv/s Figur 3.3. Plot of th pnn of pk potntil on ph for th voltmmogrm shown in Figur 3.2 ph Copyright 14 IJTEEE.
3 ITERATIAL JURAL F TECHLGY EHACEMETS AD EMERGIG EGIEERIG RESEARCH, VL 2, ISSUE 14. Efft of Conntrtion Th fft of onntrtion ws stui using yli voltmmtry. Th rsults r shown in Figur 3.4. Whn th onntrtion of silnfil itrt inrss th pk urrnt inrss sussivly Conntrtion (mm) 6 4 Pk urrnt (µ A) Squr root of sn rt ((mv/s) 1/2 ) Figur 3.4. Cyli voltmmogrm of silnfil itrt t iffrnt onntrtions ( M, M, M, M, M ) in.4 M Britton- Roinson uffr (ph = 3.26) t sn rt of mv/s Inst: Plot of pk urrnt s funtion of onntrtion Th linr pnn of pk urrnt on silnfil itrt onntrtion is shown in th inst of Figur 3.4 With R =.99 n slop of (.19). Th linr fit follows th qution: i p = [SC], whr SC is silnfil itrt. Efft of sn rt Th yli voltmmogrm of silnfil itrt solution ws run t iffrnt sn rts (Figur 3.). As th sn rt hngs from to 2 mv /s, thr is n inrs in th vlu of noi pk urrnt with onstnt noi pk potntil Figur 3.. Cyli voltmmogrm of M silnfil itrt t iffrnt sn rts.,.,. 1,.,. 2 mv/s Inst: Plot of squr root of sn rt vrsus pk urrnt Th plot of pk urrnt vrsus squr root of sn rt shows linr rltionship with R =.993. Th rsult suggst tht its ltro rtion is iffusion ontroll Eltrohmil hvior of silnfil itrt in Diffrntil Puls Voltmmtry Th iffrntil puls voltmmogrm of silnfil itrt Th ltrohmil hvior of silnfil itrt ws stui using iffrntil Puls Voltmmtry t trt GCE. It shows on irrvrsil oxition pk t out 1.23 V s shown in Figur 3.6 low. Th otin pk orrspons to th oxition of piprzin ring. Copyright 14 IJTEEE.
4 ITERATIAL JURAL F TECHLGY EHACEMETS AD EMERGIG EGIEERIG RESEARCH, VL 2, ISSUE Inst: Plot of pk urrnt s funtion of onntrtion Th linr pnn of pk urrnt on silnfil itrt onntrtion is shown in th inst of Figur.7 with R =.997 n slop of (.72). Th linr fit follows th qution: 1 i p =.4333x [SC]. Th Efft of Puls Amplitu Th fft of puls mplitu ws vri from - mv. Th Pk urrnt is inrs with n inrsing in puls mplitu. Thr ws pk roning whn th mplitu ws lss thn 4 mv n th symmtry of voltmmogrm ws not goo yon 4 mv s shown in Figur 3., s rsult, n mplitu of 4 mv ws hoosn. Th fft of puls mplitu on pk urrnt is shown in th inst of Figur Figur 3.6. Diffrntil puls voltmmogrm of.66 - M silnfil itrt t GCE in.4 M Briton-Roinson uffr (ph = 3.26) t sn rt of mv/s. Efft of Conntrtion Th fft of onntrtion n shown y roring th DPV t h onntrtion ( M). Th rsulting iffrntil puls voltmmogrm onsists of urrnt pks, th hight of whih is irtly proportionl to th silnfil itrt onntrtion s shown in th Figur Conntrtion (mm) Figur 3.7. Diffrntil puls voltmmogrm of silnfil itrt t iffrnt onntrtions ( M, M, M, M, M ) in.4 M Britton-Roinson uffr (ph = 3.26) t sn rt of mv/s Puls mplitu (mv) Figur 3.. Diffrntil puls voltmmogrm of 1.21 x -4 M silnfil itrt t iffrnt puls mplitu Inst: Plot of pk urrnt s funtion of iffrntil puls mplitu 4. Conlusion n Rommntion 4.1. Conlusion Th ltrohmil oxition of silnfil itrt in.4 M Britton-Roinson mi ws sussfully stui y ltronlytil thniqus, nmly, CV n DPV t glssy ron ltro. Svrl voltmmtri prmtrs hv n optimiz n thir influn in pk urrnt n pk potntil ws stui. Th ph fft of ltrolyt solution hs n stui in CV. As th ph inrss th noi pk potntil rstilly shift to th ngtiv potntil. Copyright 14 IJTEEE.
5 ITERATIAL JURAL F TECHLGY EHACEMETS AD EMERGIG EGIEERIG RESEARCH, VL 2, ISSUE Rommntion Th ltrohmil hvior of Soli silnfil itrt hs n trmin using CV n DPV thniqus. Th mthos n us for th qulittiv n quntittiv trmintion of silnfil itrt in givn phrmutil smpls.. Rfrns [1] Boolll, M.; Gpi-Att, S.; Gingll, J. C.; Alln, M.J. Silnfil, novl fftiv orl thrpy for ml rtil ysfuntion. Br. J. Urol. 1996, 7, [2] Fishr, H.; Romr, A.; Ulrih, Arns, H. Plnt M. 19, 39 [3] Gu, Z.; Zhng, D.; Yng, X.; Httori, M.; m, T. Chm. Phrm. Bull. 199, 3, 249 [4] Bur, A. W.; Kiry, W. M.; Shrris, J. C.; Truk, M. Am. J. Clin. Pthol. 1996, 4, 493 [] Klyonuoglu,.; Rolls, S.; Sur-Altinr, D.; Ygnoglu.Y.; Ang,. Phrmzi.1992, 47,796 [6] Mhmoo, K.T. n Khn, B. Pkistn J. Si., 62 [7] Flmn, H. A.; Golstin, I.; Htzihristou, D. G.; krn, R. J.; Mkinly, J. B. J. Urol. 1994, 4,11. [] Kling, J.; "From hyprtnsion to ngin to Vigr". Mo Drug Disov. 199, 1, [9] [] Dinsh,. D.; grju, P.; M Gou,. M.; Rngpp; Tlnt. 2, 7, [11] El-Giny, A. E.; Shokry, E. M.; Frouk, L.; A El- Aziz, J. J. Biom Si n Rs., 2, [] Brtt, M.A. Eltrohmistry Prinipls Mthos n Applitions, 1 st.; xfor Univrsity Prss, w York, 1993, 1-3 Copyright 14 IJTEEE.
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