Research Progress on the A ssay of D eterm ina tion of Am inoglycoside Residues in An ima l - der ived Food

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1 45 1, 2, 2, 2, 1, 3, 2, 2, 2 (1., ; 2., ; 3., ) [ ] [ ] A [ ] (2008) [ ] S [ ],, [ ] ; ; Research Progress on the A ssay of D eterm ina tion of Am inoglycoside Residues in An ima l - der ived Food AN Dong 1, 2, WANG Guo - m in 2, L I Xian - liang 2, ZHANG J in - zhong 1, 3, L I Ying - guo 2, ZHANG Lei 2, ZHOU Q i - m ing 2 ( 1. College of Resources and Environm ent, Southwest U niversity, Chongqing ; 2. Chongqing Entry - Exit Inspection and Quarantine B ureau, Chongqing ; 3. Chongqing Key Laboratory of Agricultural Resources and Environm ent, Chongqing ; China) Abstract:Am inoglycosides is a kind of w idely used antibiotics in stockbreeding. The residues in anim al - derived food m ay be severely harm ful for hum an health. In this paper, the m ain detection methods for am inoglycoside resi2 dues in anim al - derived food were reviewed, and the development trend was forecasted. Key words: am inoglycosides; residue detection; anim al - derived food (Am inoglycosides), [ 1 ] [ 2 ] (MRL ) 200 g/kg; MRL s 0. 6 mg/kg [ 3 ] ; 1997, MRL s 0. 2 : (2006BAK02A08-6) : (1983 2),,, :, E - mail: jzhzhang@ swu. edu. cn

2 , 42 (10) : /, mg/kg; ( FDA) MRL mg/kg; FAO (WHO ) ( CAC) MRL s 0. 5 mg/kg 0. 2 mg/kg [ 4 ], 1,,, 1. 1 ( EL ISA ) - J in [ 5 ],, 0. 5 g/l, 112% Loomans [ 6 ], EL ISA, g/l Xiao [ 7 ], 2. 7 g/kg Baxter [ 8 ], 106%,, 5 m in, 4. 1 g/l 2005, 14 EL ISA 4, [ 9 ], J in, ; Baxter, 1. 2 (R IA ),,, [ 10 ] EL ISA R IA EL ISA, 0. 5 g/l g/l, mg/kg; R IA, 25 g/l 500 g/l, 0. 2 mg/kg MRL [ 11 ], -,, 0 50 g/l 0 25 g/l EL ISA, R IA,,,,, 2, ( SN ), 0. 5 mg/kg, 83. 7% 97. 0% [ 12 ] 10%,,, 3 ( mg/kg) 97. 6% [ 13 ],, mg/kg TTC ( Tripheye tetrazollium chloride), (S. therm ophilus) 2, 3, 5 - ( TTC),, TTC [ 14 ] TTC, mg/kg TTC,

3 47,,,,,,, ( GC),,, GC, Preu [ 15 ] ( TMSI) (OPA) 9 - ( FMOC - Cl) 1, (NQS), [ 16 ] NaOH NQS, ( 263 nm 447 nm), 87. 9% 93. 8%, [ 17 ] HClO 4, C 18,,, mg/kg [ 18 ],, Edder [ 19 ] HClO 4, C 18 ( SPE), NaOH, HPLC - FD ( 260 nm, [ 20 ], mg/kg Fennell [21 ] KH 2 PO 4 - Na 2 SO 4 (ph 8. 8), HPLC - UVD ( 330 nm ), 0. 6 g/kg 1 g/kg [ 22 ] H 3 PO 4,, mg/kg [ 23 ], ODS - C 18, NQS, HPLC - UVD (252 nm ), 120 g/kg 123 g/kg 218 g/kg Posyniak [ 24 ] (HFB IO),, HPLC - FD ( 260 nm,, nm), mg/kg ( GC - ECD ),, g NaOH,,, 3. 2 (HPLC) [ 25 ],,, HPLC, 5 g/kg V inas [ 26 ] NQS,, ( ) 5%,,,,, [ 27 ],, mg/kg 50. 3% 76. 9% B raganoski [28 ] HPLC,, g/ml HPLC, [ 29 ], ( ELSD) [ 30 ] HPLC, ph, HClO 4 ;,,, SPE C 18, ; 435 nm ), mg/kg,,

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