The Era of Mass Surveillance

Transcription

1 The Era of Mass Surveillance Identify Suspects, Confirm Targets and Explore Unknowns with GC/Q-TOF Nathan Eno GC/Q-TOF Product Manager Kai Chen, Ph.D. GC/MS Application Scientist 1

2 Panoramic Technology for Full Spectrum Surveillance 2

3 High Resolution and Mass Accuracy Reproducible Spectral Performance Simultaneous High Resolution and Wide Dynamic Range Flexible Agilent 7890B GC Sensitive Low Energy EI 3

4 MassHunter Acquisition MassHunter Qualitative Analysis MassHunter Quantitative Analysis MassHunter PCDL Manager Online Resources MassHunter Qualitative Analysis Molecular Structure Correlator Actionable Results MassHunter Unknowns Analysis MassHunter Profinder / Unknowns Analysis Mass Profiler Professional 4

5 Target Quantitation Linearity and Detection Limit Performance for Targeted Assays 5

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7 Responses 6-Hydroxychrysene - 9 Levels, 9 Levels Used, 10 Points, 10 Points Used, 0 QCs x10 6 y = * x Urine R^2 = Type:Linear, Origin:Ignore, Weight:None Responses OH 6-Hydroxychrysene (TMS derivatized) LOD = 0.14 ng/ml Concentration (ng/ml) 6-Hydroxychrysene - 9 Levels, 9 Levels Used, 9 Points, 9 Points Used, 0 QCs x10 6 y = * x R^2 = Plasma 4 Type:Linear, Origin:Ignore, Weight:None LOD = 0.16 ng/ml Concentration (ng/ml) LOD (ng/ml) per matrix Compound name Urine Plasma Naphthalene Acenaphthylene Acenaphthene Hydroxynaphthalene Hydroxynaphthalene Fluorene Phenanthrene Anthracene ,6-Dihydroxynaphthalene ,7-Dihydroxynaphthalene Hydroxyfluorene hydroxyfluorene Hydroxyphenanthrene Fluoranthene Hydroxyphenanthrene Hydroxyphenanthrene Hydroxyphenanthrene Pyrene Benzo(a)anthracene Chrysene Hydroxychrysene Benzo(b)fluoranthene Benzo(k)fluoranthene Benz(a)pyrene Indeno(1,2,3 -cd)pyrene Dibenz(a,h)anthracene Hydroxybenzo(a)pyrene Benzo(ghi)perylene ,8-Hydroxybenzo(a)pyrene

8 SureMass Signal Processing Ridge Feature RT m/z Conventional Deconvolution (Nominal Mass) SureMass Signal Processing (Profile Accurate Mass) 8

9 Suspect Screening Analytical Performance Factors for Confident Compound Detection Fingerprint image adapted from The Photographer - Own work, CC BY-SA 3.0, 9

10 Suspect Surveillance Now with even broader scope GC/Q-TOF Pesticides & Environmental Pollutants PCDL now with compounds: High Resolution Spectra Expert, human curation Better compound alignment with US EPA 8270 targets and Agilent GC/TQ MRM database PAHs Organophosphates Amines Phthalates Nitroanilines Chloronitrobenzenes 10

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12 solvent Clostebol Metabolite (TMS-derivatized) 0.29 ppm 0.21 ppm 0.69 ppm 0.51 ppm urine 5 ng/ml (MRPL) 0.56 ppm ppm 0.25 ppm 0.08 ppm 12

13 0.3 ppm 13

14 Unknowns Identification Extensive Functionality and Tools for Identification and Elucidation 14

15 70 ev ev Candidate: Escitalopram 1512 ev ev ev ev 15

16 High Strength Axial Magnet Centered Filament Optimized Lens Design Proprietary Low Energy EI Source 16

17 70 ev 17 ev 15 ev Clenbuterol (TMS derivatized) Clenbuterol ( ), MRPL = 0.2 ng/ml 70 ev 17 ev 15 ev Stanozolol (TMS derivatized) 4-OH-Stanozolol ( ), MRPL = 5 ng/ml 17

18 TIC 12eV 70eV diaromatics C 14 H 12 O 3.3 ppm 0.65 mda Library search: 4-(1-methylethyl)-1,1'-Biphenyl (C 15 H 16 ) C 15 H ppm 0.56 mda June 2018 The Era of Mass Surveillance Agilent Public For Research Use Only. Not for use in diagnostic procedures.

19 - CH 3 MS/MS Precursor: 196 CID 10eV C 14 H 12 O 0.34 ppm 0.07 mda C 15 H ppm 0.25 mda C 13 H 9 O 0.21 ppm 1.15 mda C 14 H ppm 0.48 mda MS/MS Precursor: 196 CID 10eV C 14 H 12 O 0.85 ppm 0.17 mda - CH 3 C 15 H ppm 0.05 mda C 13 H 9 O 0.21 ppm 1.15 mda C 14 H ppm 0.12 mda June 2018 The Era of Mass Surveillance Agilent Public For Research Use Only. Not for use in diagnostic procedures.

20 Compound ID =??? 70 ev matrix: packaging extract 20

21 70 ev matrix: packaging extract 10 ev matrix: packaging extract M + candidates 21

22 70 ev matrix: packaging extract Candidate #1 Candidate # X N/A

23 70 ev matrix: packaging extract Low Energy EI (10 ev) Formula: C 15 H 24 O Mass : -1.5 ppm M CID@5eV M+1 M+2 23

24 0.7 ppm 0.6 ppm 1.0 ppm 2.4 ppm 0.4 ppm Candidate: Benzenemethanol derivative 24

25 Case Study: Food Safety Full Spectrum Accurate Mass Data for Targets, Suspects and Unknowns 25

26 Safeguarding food supply is challenging pesticides in use or remain in environment Other environmental pollutants are also of concern Thousands of possible contaminants/food pairs High sensitivity and selectivity needed to meet Default MRLs in dirty extracts Growing interests in broadest scope and even untargeted screening for risk assessment 26 August 22, 2018 Agilent Technologies, Inc.

27 Challenges for our customers How much of each calibrated target compound is present? Are other target residues present? Is there anything else in my sample? Dozens of Compounds e.g., 100~200 Targets targets 100 s of Compounds e.g., 800 Targets 1000 s of Compounds 27

28 Workflow Strategy Acquire full-spectrum data Yes Targeted Method Calibrate? No Targeted Quantitative Screening Suspect Screening Untargeted Screening Deconvolution followed by matching public libraries 28

29 Configuration Optimized with Backflushing PUU: Purged Ultimate Union Mid-column back flushing system Q-TOF Reduced run times Longer column lifetime Less ion source contamination 29 August 22, 2018 Agilent Technologies, Inc.

30 7250 GC/Q-TOF Method Parameters Agilent 7890 GC Parameter Inert flow path configuration Columns Inlet Value Injection volume 1~2 µl Injection mode Mid-column backflush Agilent HP-5ms UI, 15 m, 0.25 mm id, 0.25 μm film (two each) MMI, 4 mm UI liner single taper w wool Cold Splitless 60 C for 0.2 minutes 600 C/min to 300 C, hold 330 C, post run Agilent 7250 Q-TOF Parameter Source temperature Value Quad temperatures 150 C Collison cell gas flows Electron energy Acquisition mass range Spectral acquisition rate 280 C (70 ev), 250 ( C) 1 ml/min N 2 4 ml/min He 70 ev (Standard EI), 15 ev (Low energy EI) m/z 5 spectra/sec Inlet flow (column 1) PUU flow (column 2) Oven temperature program 1.0 ml/min (Chlorpyrifos-methyl locked at min) column 1 flow ml/min 60 C (hold 1 min) then 40 C/min to 170 C, then 10 C/min to 310 C (hold 3 min) Run time min Transfer line 280 C Midcolumn Backflush Timing 5 min duration during post-run Oven temperature 310 C Aux EPC pressure ~50 psi Inlet pressure ~2 psi

31 Method Evaluation Targeted Quantitative Screening 31

32 Sample Preparation Matrix Extraction dspe Cleanup QuEChERS (EN) EMR-Lipid QuEChERS (EN) Pigment matrix QuEChERS (EN) Fruit/Vegetable QuEChERS (EN) Fruit/Vegetable Blank food matrices spiked with pesticides for method development and calibration A mixture of 140+ pesticides represent OCs, OPs, carbamates, triazoles and pyrethroids, etc. 6 replicates 32 August 22, 2018 Agilent Technologies, Inc.

33 Matrix Complexity Increases Replicate Precision Targeted Quantitative Screening x x x ng/ml, n = x Counts vs. Acquisition Time (min) 33 August 22, 2018 Agilent Technologies, Inc.

34 Matrix Matched Calibration Targeted Quantitative Screening ng/ml α-bhc (Organochlorine) +30% α-bhc 5 ng/ml Carbofuran 5 ng/ml Carbofuran (Carbamate) +30% Lib Lib -30% -30% RSD: 3.7% RSD: 3.8% +30% Malathion (Organophosphorus) Malathion 5 ng/ml Deltamethrin 5 ng/ml Deltamethrin (Pyrethroid) +30% Lib Lib -30% -30% RSD: 7.2% RSD: 2.7% 34 August 22, 2018 Agilent Technologies, Inc.

35 Confidence Enhanced by Mass Accuracy Targeted Quantitative Screening 5 ng/ml 35 August 22, 2018 Agilent Technologies, Inc.

36 Identify Suspect and Confirm Target Targeted Quantitative Screening Suspect Screening 36

37 Guideline to Identify Compounds EU SANTE/11813/2017 Guidelines 37 8/22/2018

38 How to Confirm Identification Suspect Screening RT diff Mass accuracy S/N EIC Alignment Fragment Ratio 38 8/22/2018

39 Targeted Screening Real samples from the Supermarket Targeted Quantitative Screening Suspect Screening Sample Compound name RT diff Score Mass diff Amount (min) Coelution Frag ratio (ppm) (ng/ml) Sample Compound name RT diff Score Mass diff Amount (min) Coelution Frag ratio (ppm) (ng/ml) 2,6-Dimethylphathalene ID only 1,2,3,5-tetrachlorobenzene ID only Azobenzene ID only 1-aminonaphthalene ID only Benzylbenzoate ID only Anthraquinone ID only Biphenyl ID only Azobenzene ID only Boscalid ID only Azoxystrobin DPA / Diphenylamine ID only Benzyl benzoate ID only Eugenol ID only Boscalid ID only Fludioxonil ID only Cyfluthrin Flutriafol ID only Cyhalothrin Phenanthrene ID only Chlorthal-dimethyl ID only Pyraclostrobin ID only Dimethomorph (E & Z) Pyrimethanil ID only Fludioxonil ID only Thiabendazole ID only Metalaxyl ID only 2,6-Dimethylphathalene ID only p,p'-dde Anthraquinone ID only Pentachlorobenzonitrile ID only Imazalil ID only Permethrin Propiconazole ID only Phenathrene ID only Pyrimethanil ID only Pyraclostrobin ID only Thiabendazole ID only Thiabendazole ID only Thiamethoxam Thiamethoxam Tri(2-chloroethyl)phosphate ID only Triphenylmethane ID only 39 August 22, 2018 Agilent Technologies, Inc.

40 Explore Unknown Untargeted Screening 40

41 Counts Untargeted Screening (NIST) + TIC Scan Broccoli non-org 70eV8uA280C 02.D x Untargeted Screening Acquisition Time (min) C15H12O ppm C10H5Cl3-106 ppm NIST Search Score: 89.8 Methyl-2-benzoylbenzoate NIST Search Score: ,3,7-Trichloronaphthalene 41 August 22, 2018 Agilent Technologies, Inc.

42 Elucidate structures.. low energy EI, MS/MS and accurate mass Untargeted Screening Step 1: Confirm M + Step 2: Confirm fragment ions Full spectrum acquisition Step 3: Structure elucidation on candidate MS/MS CID@25 ev Low energy EI (15 ev) C8-H-Cl3-N2 turns out to be 2,4,5-Trichloroisophthalonitrile. That is not NIST. It is in fact a degradation product of Chlorothalonil 42 August 22, 2018 Agilent Technologies, Inc.

43 7250 GC/Q-TOF Mass Accuracy Isotopic Fidelity MS/MS Performance Low Energy EI Capability MassHunter Software Identify. Quantify. Simplify Image Credit: Surveillance by Nick Youngson CC BY-SA 3.0 Alpha Stock Images 43

44 Thank You! MP 238 Analysis of polycyclic aromatic hydrocarbons (PAH) and hydroxylated PAH metabolites in plasma and urine MP 284 Screening of contaminants in food and natural products TP 180 Low Energy EI and High Resolving Power Instrumentation for the Analysis of Arson Samples TP 213 Targeted and Untargeted Analysis of Pesticides and Other Contaminants in Fruits and Vegetables WP 280 Analysis of doping and forensic drugs in urine ThP 286 Analysis of halogenated polycyclic aromatic hydrocarbons in atmosphere ThP 295 Combination of chemical ionization (CI) and low electron energy ionization capabilities 44